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Phragmipedium kovachii is a species of orchid endemic to the Amazonas and San Martín regions. Unfortunately, its excessive extraction has made it a critically endangered species. In this study, we performed next-generation sequencing of P. kovachii (GenBank accession number OR348669) and assembled its complete chloroplast genome. The complete chloroplast genome of P. kovachii is A + T-rich (64.3%), measuring 152,918 bp in length. This plastid genome contains a total of 124 genes (77 protein-coding genes, 39 tRNAs, and eight rRNAs) and five pseudogenes, including a pair of inverted repeats (IRs) 25,116 bp in size and separated by a large single-copy (LSC) region of 89,216 bp and a small single-copy (SSC) region of 13,470 bp. This genome has a typical quadripartite organization following the structure of other Orchidaceae plastomes. Phylogenetic analyses revealed the close relationship between P. kovachii and P. besseae. This study contributes to the understanding of the phylogenetic relationships of the monophyletic group Cypripedioideae.

Participatory Geographic Information Systems (PGIS) combine geographic information with citizen or community participation to integrate local knowledge with issues related to natural resource management, territorial planning, and environmental pollution that are fundamental to decision-making. However, the current challenge is involving and educating the population to succeed in the PGIS. For this reason, a PGIS system was implemented to identify areas with higher solid waste accumulation and to determine the accuracy of the reports made by the trained population. A participatory platform was implemented using ArcGIS (Online, Desktop, Survey123, Web App Builder, and Experience Builder) for daily reporting of areas contaminated by solid waste. The information obtained for two months was used to generate Kernel density maps, which made it possible to identify the most contaminated regions of the city. A total of 2778 areas contaminated by solid waste were reported during the evaluation period, where 53 % (1486) presented a low level of contamination (area less than 1 m2), 16 % (442) had a medium level of contamination (area equal to 1 m2) and 31 % (850) reported a high level of contamination (area greater than 1 m2). The results allowed the generation of Kernel density maps showing 24 areas classified as 'Extremely High'. Saturdays and Sundays reported higher contamination concerning the other days of the week. The accumulation of contaminants is mainly in the water bodies located in the study area.

This study evaluated the influence of Eucalyptus globulus L. plantations on the physical, chemical and biological properties of the soil in three districts: Magdalena, Tingo and San Isidro del Maino. The evaluation was carried out in six plots, three with eucalyptus and three with natural forests. In each plot with eucalyptus, 10 specimens were selected to measure their dasometric characteristics. Ten soil samples were taken at the base of each specimen to evaluate the bulk density and soil characterization, replicating the sampling in the natural forest plots. Additionally, biological sampling was carried out in each plot using the Rapid Soil Sampling Protocol, with a total of five quadrats per plot. The results showed significant differences in diameter at breast height (DBH), tree height and dry biomass among the districts evaluated. Trees in Magdalena presented the highest DBH and height, with an average biomass of 934.22 kg/tree, while those in Tingo showed the lowest values, with an average biomass of 230.00 kg/tree. Diverse flora species associated with both eucalyptus and natural forests were identified, reflecting a rich biodiversity in each district. Soils with eucalyptus in Tingo showed a predominantly clay texture, while in Magdalena and San Isidro del Maino a sandy loam texture predominated. In the natural forests, the soils in Tingo were also clayey, with more varied textures in the other districts. Bulk density was higher in forest soils in Tingo and in eucalyptus soils in San Isidro del Maino. Soils under eucalyptus had lower pH and lower electrical conductivity compared to natural forest soils. In terms of nutrients, the eucalyptus soils in Tingo and Magdalena showed higher phosphorus and potassium contents, while in San Isidro del Maino, the forest soils had higher levels. In general, eucalyptus soils showed higher levels of organic carbon, organic matter and nitrogen in Tingo and Magdalena, but lower in San Isidro del Maino. Biologically, natural forest soils exhibited a higher diversity and quantity of organisms compared to eucalyptus soils, especially in San Isidro del Maino. Detritivore indices were higher in natural forests, suggesting greater soil biodiversity. This higher diversity could be associated with better soil quality in these systems, highlighting the importance of natural forests in maintaining healthy soils.

Coffee (Coffea arabica L.) is a globally important agricultural crop with increasing quality requirements. However, the challenges posed by climate change impact cup quality. Therefore, the objective was to evaluate the influence of tree diversity and crop segmentation under agroforestry systems on the sensory characteristics of coffee. The study employed a 4A*3B factorial design, with factor A: agroforestry systems (mixed system, shade-free system, system with Erythrina edulis and system with Inga edulis) and factor B: segmented harvest (upper, middle, and lower third cherries). Fragrance/aroma, flavor, residual flavor, acidity, body, uniformity, balance, clean cup, sweetness, cupper's score, and final score were evaluated. The results suggest that coffee trees planted in mixed systems provide the best sensory characteristics of coffee in the cup since the highest averages were obtained in most of the variables evaluated. Likewise, it is concluded that the diversity of trees (mixed system and system with Erythrina edulis) has a positive influence on the sensory quality of the coffee, reaching a score of 82.86 and 82.64 for the mixed system and the system with Erythrina edulis respectively; categorizing them as specialty coffees. Agroforestry systems constitute an option for developing sustainable agriculture while improving the sensory quality of coffee in the Peruvian Amazon.

Abstract The study examined the efficacy of freeze-dried papain enzyme obtained from three Vasconcellea species (V. pubescens, V. chachapoyensis, V. heilbornii) as a natural coagulant in cheese making. Notably, the enzyme V. pubescens demonstrated the most promising results when concentrations of 2 g/L, 4 g/L, and 6 g/L were used to produce fresh cheese, while other enzyme species exhibited lower efficacy. The optimal yield of fresh cheese with minimal residual enzyme was achieved when a 2 g/L dose of papain enzyme was employed at a coagulation temperature of 30 °C, resulting in physicochemical and organoleptic characteristics comparable to those produced with commercial Hansen’s rennet. Nevertheless, an increase in the coagulation temperature (42 °C) and a higher dose of papain enzyme (4 g/L) resulted in a reduction in the yield of fresh cheese and; consequently, the residual enzyme increased. Further studies are required to determine the purity of freeze-dried papain and the most effective dosage to increase profitability for producers and consumers. Such findings could facilitate the ecological application of this alternative in producing of fresh cheese.

Introduction Expansion sphincter pharyngoplasty has been shown to be a good alternative to continuous pressure devices in patients with moderate to severe obstructive sleep apnea. On the other hand, drug-induced sleep endoscopy provides information on the pattern of collapse in obstructive sleep apnea, although it is unclear whether this information improves the surgical outcomes.

Abstract The Peruvian Andes are the natural habitat of several wild blackberry species that are little known and exploited due to the lack of technological and scientific development to support their agricultural potential. In this context, a study was conducted to understand the physicochemical composition, bioactive compounds, antimicrobial activity, and in vitro multiplication of four wild blackberry (Rubus sp.) species from the northern Peruvian highlands. The results indicate that fruits of R. floribundus presented the highest content of total soluble solids (9.58 ± 1.83°Brix) and titratable acidity (1.88 ± 0.07% citric acid). The fruits of R. weberbaueri recorded the highest total phenolic content (415.06 ± 8.69 mg GAE/100 g Ff). The antioxidant capacity determined by the DPPH assay varied significantly among species, with the highest value found in fruits of R. andicola (50.27 ± 0.11 mg TE/100 g Ff). The fruit extracts of R. weberbaueri and R. andicola showed better antimicrobial activity, with Staphylococcus aureus being the most sensitive bacterium. In the in vitro multiplication phase, the results show that BAP (6-Benzylaminopurine) has a significant effect at a dose of 1.5 mg l−1 on shoot number, leaf number, and shoot length. The results may help in the management of genetic resources.

AbstractTheobroma bicolor Bonpl. 1806 is distributed in the Neotropics from southern Mexico to the Peruvian and Brazilian Amazon. High-throughput sequencing of T. bicolor from Peru (KUELAP2926) resulted in the assembly of its complete plastid genome (GenBank accession number OQ557154). The chloroplast genome of T. bicolor is A + T-rich (62.97%), having 160,317 bp in size and containing 130 genes; including a pair of inverted repeat regions (IRs) of 25,462 bp separated by a large single copy region (LSC) of 89,221 bp and a small single copy region (SSC) of 20,172 bp. This plastid genome is similar in length, content, and organization to other members of the genus Theobroma. Phylogenetic analyses of T. bicolor support its sistership to the clade comprising T. cacao and T. grandiflorum. This study may contribute valuable information to the phylogenetic relationships within the genus Theobroma.

Wildfire represents a significant threat to ecosystems and communities in the Department of Amazonas, Peru, causing losses in biodiversity and land degradation and affecting socioeconomic security. The objective of this study was to analyze the spatial and temporal distribution of burned areas (BAs) from 1986 to 2023 to identify recurrence patterns and their impact on different types of land use and land cover (LULC). Landsat 5, 7, and 8 satellite images, processed by Google Earth Engine (GEE) using a decision tree approach, were used to map and quantify the affected areas. The results showed that the BAs were mainly concentrated in the provinces of Utcubamba, Luya, and Rodríguez de Mendoza, with a total of 1208.85 km2 burned in 38 years. The most affected land covers were pasture/grassland (38.25%), natural cover (forest, dry forest, and shrubland) (29.55%) and agricultural areas (14.74%). Fires were most frequent between June and November, with the highest peaks in September and August. This study provides crucial evidence for the implementation of sustainable management strategies, fire prevention, and restoration of degraded areas, contributing to the protection and resilience of Amazonian ecosystems against future wildfire threats.

Cattle ranching is a fundamental economic activity in northern Peru, where proper management of water resources is crucial. This study, a pioneer in the region, evaluated water quality and its suitability for human consumption, vegetable irrigation, and livestock production. It is also the first study to document the presence of metals and metalloids in vulnerable areas because they are located at the headwaters of river watersheds. The spatiotemporal evaluation of physicochemical parameters, metals, and metalloids was performed in five micro-watersheds (Cabildo, Timbambo, Pomacochas, Atuen, and Ventilla) from water samples collected in the dry season (October 2017) and wet season (March 2018). The parameters were analyzed using microwave plasma atomic emission spectrometry. The results were contrasted with international and Peruvian quality standards related to dairy cow production. The highest values of pH, total dissolved solids, and electrical conductivity were reported during the dry season, and the highest turbidity during the wet season. Of the metals evaluated, arsenic (As) was omnipresent in all the micro-watersheds, followed by lead (Pb).In contrast to World Health Organization regulations, concentrations of As, cadmium (Cd), Pb, and iron represent a risk; according to Peruvian regulations, As and Pb exceed the concentrations established for use in animal drinking water and vegetable irrigation, and according to water guidelines for dairy cattle, concentrations of As, Pb, Cd, and Al exceed the permitted limits. The high concentrations of these metals in the study area are attributable to a synergy between natural factors, such as Andean geology and livestock activity. The data reported will allow for proper water resource management, pollution prevention, and the design and adoption of mitigation measures.

The objective was to evaluate the ecology and dynamics of the native species community in the Bosque Palmeras de Ocol in the district of Molinopampa, Amazonas, Peru. Six sampling points were established, in which 1,569 trees belonging to 47 species were found. Species such as Astrocaryum sp., with a relative density of 10.7 % and an importance value index (IVI) of 23.54 %, and Prunus sp., with a relative density of 8.9 % and an IVI of 22.23 %, stood out. Myrsine coriacea subsp. 2 was identified as key with a high relative frequency and an IVI of 16.21 %. Density per hectare revealed high values for Astrocaryum sp. (560 individuals/ha), Nectandra sp. (526 individuals/ha) and Prunus sp. 2 (470 individuals/ha). The distribution of tree height and diameter showed a predominance of smaller individuals, with most species presenting diameters less than 20 cm and heights between 3 and 4 m. The range/abundance curve highlighted Astrocaryum sp. as the most abundant species. The distribution models showed a good fit to the Null and Preemption distributions, with low values of Akaike's criterion (AIC) 111.56 and 114.60, respectively. These findings provide a detailed understanding of the species community in this forest, fundamental for its conservation and sustainable management.

Currently there are problems in improving the competitiveness of the coffee industry in Amazonas, in this context the present research seeks to analyze the future behavior of the coffee industry through a structural analysis with MICMAC. For this purpose, 14 coffee guilds of Amazonas were interviewed, followed by an external and internal evaluation and a structural analysis with MICMAC. Subsequently, future events were analyzed and validated by experts. MICMAC identified in the short term the importance of local and regional governments supporting the coffee associations in order to achieve their objectives, such as increasing organic coffee production and expanding the coffee frontier; in the medium term, a coffee research center should be created; and in the long term, studies on the different microclimates should be carried out in order to achieve a high price and international demand. The motricity and dependency study contributed to the generation of eight probable events, of which five events are likely to occur by 2030 and a desirable scenario is the ideal.

Phaseolus vulgaris L. is a legume of high nutraceutical value, widely cultivated and consumed. However, common bean production faces challenges such as water stress that severely affects its growth and yield. This study evaluated the morphological and physiological response of four native P. vulgaris accessions subjected to different irrigation treatments under greenhouse conditions. A completely randomized design with factorial arrangement was used, evaluating three irrigation frequencies (100%, 50%, 25%) in combination with four accessions (PER1003541, PER1003542, PER1003543, PER1003544). The results showed that with the 25% irrigation treatment, PER1003544 showed a 54.62% decrease in leaf area, while PER1003542 and PER1003543 experienced reductions of 56.56% and 59.24%, respectively. In addition, accession PER1003544 reported a smaller reduction in the number of flowers and pods, with decreases of 40.21% and 29.9%, in contrast to PER1003543, which showed decreases of 60.66% and 52.63%, respectively. Accessions PER1003541 and PER1003544 also recorded the lowest reductions in dry biomass, with 31.85% and 35.41%, respectively. Regarding yield, PER1003544 and PER1003541 experienced reductions of 59.01% and 69.79%, respectively, unlike PER1003543, which showed a 90% decrease. In relation to stomatal density, PER1003541 recorded a reduction of 28.28%, while PER1003544 had a decrease of 37.10%, and PER1003543 experienced a reduction of 47.05%; chlorophyll content showed a similar trend. Finally, PER1003544 maintained a relatively stable stomatal index, with a reduction of 29.01%, compared to PER1003543, which reduced by 60.99%. In conclusion, accession PER1003544 stands out as a promising variety for breeding programs focused on water stress tolerance, contributing to food security and agricultural sustainability in areas affected by limited water availability. However, PER1003541 would be a suitable additional option, offering farmers flexibility in their crop selection according to the specific conditions of their environment.

The management of organic waste through anaerobic digestion is an alternative to energy recovery. This research focused on evaluating the production of biogas with different inoculums. For this purpose, two types of systems were implemented—one used a heating system controlled by an STC-1000 thermostat, while the other used a solar heating system under a polycarbonate parabolic trough. The experiment was carried out at laboratory level with 3 L PET bottle biodigesters and the biogas produced was collected with the water displacement technique in 3 L bottles, calibrated every 50 mL, over 43 days. Inoculums of the following manure concentrations were used: water (1:5, 1:2, 1:3) mixed with Caesalpinia spinosa meal and molasses. The results determined that the thermostat-controlled heating system generated 69.6 mL/day of biogas while the other system produced 610.9 mL/day. On the other hand, the T1 treatment with a manure:water ratio of 1:5 and molasses and Caesalpinia spinosa meal inoculums in both systems had a higher average biogas volume. In terms of methane (CH₄), the highest value of 76.9% was obtained through the T1 treatment under the controlled heating system. This allows the production of biogas with a high concentration of methane, which in future applications can be utilized for residential or industrial purposes, promoting economic, social and environmental development. Since the main challenge in the production of biogas is to reduce the digestion time, which is influenced by the temperature of the site, two types of inoculums with a low cost and easy access were used.

Factors such as the selection of cultivars and the planted density affect the development and yield of peanuts (Arachis hypogaea L.). This study’s objective was to evaluate peanut cultivars’ agronomic behavior under three planting densities in the northeast of Peru. The design used was randomized complete blocks (DBCAs) with a bifactorial arrangement 4A × 3B (factor A, peanut cultivars; factor B, planting densities), forming 12 treatments with three replications per block. The results revealed that T3 (Huayabamba cultivar + density of 30 × 50 cm) stood out, presenting the most favorable means in the number of pods (16 pods), number of seeds per pod (five seeds), height at 90 days (22.7 cm), and yield (1850 kg/ha). Empty pods did not show significant differences between treatments. T8 (Chivita cultivar + density of 20 × 50 cm) indicated the highest number of branches (six branches); in the weight of 100 seeds, the Rojo Tarapoto cultivar was the most encouraging, adapting optimally to the three densities. In addition, T7 (chivita cultivar + density of 10 × 50 cm) showed the shortest days at flowering and harvest, with 64 and 134 days. The study showed that T3 was the most efficient in pod and seed production, making it crucial to optimizing peanut yield.

The rapid development of digital tools for crop management offers new opportunities to mitigate the effects of climate change on agriculture. This study examines the Normalized Difference Vegetation Index (NDVI) in coffee-growing areas of the province of Rodriguez de Mendoza, southern Peru, from 2001 to 2022. The objectives were the following: (a) to analyze NDVI trends in these areas; (b) to investigate trends in climatic variables and their correlations with altitude and NDVI; and c) to develop linear models tailored to each coffee-growing area. The study identified significant differences in NDVI trends among coffee plants, with mean NDVI values ranging from about 0.6 to 0.8. These values suggest the presence of stress conditions that should be monitored to improve crop quality, particularly in Huambo. Variability in rainfall, maximum and minimum temperatures, relative humidity, and altitude was also observed, with NDVI values showing a strong negative correlation with altitude. These results are crucial for making informed strategic decisions in integrated crop management and for monitoring crop health using vegetation indices.

The thread blight disease (TBD) of cacao (Theobroma cacao) in the department of Amazonas, Peru was recently reported to be caused by Marasmius tenuissimus (sect. Neosessiles). This same species is known to be the main causal agent of TBD in West Africa. However, some morphological characteristics, such as the presence of rhizomorphs, the almost exclusively white color, and pileus sizes less than 5 mm, among others, differ to the description of M. tenuissimus. Therefore, we aimed to conduct a taxonomic revision of the cacao-TBD causal agent in Peru, by using thorough micro and macro morphological, phylogenetic, and nuclear and mitochondrial genomic approaches. We showed that the causal agent of TBD of cacao in Amazonas, Peru, belongs to a new species, Marasmius infestans sp. nov. This study enriches our knowledge of species in the sect. Neosessiles, and strongly suggests that the M. tenuissimus species complex is highly diverse.

Fourteen species of the genus Epidendrum, recently collected in the Área de Conservación Privada La Pampa del Burro (ACPPB), five of them new to science (Epidendrum echinatiantherum sp. nov., E. imazaense sp. nov., E. parvireflexilobum sp. nov., E. rosulatum sp. nov., and E. ochrostachyum sp. nov.), are described and illustrated. The other species include a new record for Peru (E. acrobatesii) and four for the department of Amazonas (E. brachyblastum, E. forcipatum, E. mavrodactylon, and E. tridens). Epidendrum enantilobum is here considered a synonym of Epidendrum brachyblastum. The type locality of Epidendrum cryptorhachis, originally stated as Ecuador, Guayabamba, is corrected to indicate that it refers to the valley of Guayabamba, Rodríguez de Mendoza, Amazonas, Peru. Our results show the need to continue conducting botanical exploration in the ACPPB as a baseline for subsequent studies, including a full inventory of the orchid diversity.

Entomopathogenic fungi represent the pinnacle of efficacy among biological control agents when combating insect pests within natural ecosystems, combating them without altering the environment. This study aimed to isolate and characterize the morphology, physiology, and pathogenicity of native isolates of Metarhizium sp. isolated from soil samples from the province of Rodriguez de Mendoza. Eighteen native isolates of Metarhizium sp. were isolated and characterized. Colony coloration varied between yellow-gray, white, brown, and olive, with feathery or wavy edges. As for radial growth, the highest averages were obtained by isolates LLM-M2 and TOR-M16, with 43.15 mm and 42.85 mm, respectively. Conidia production at 15 days was higher for isolate LLM-M2 with 9.8 × 107 conidia/mL; in the percentage of germination at 14 h, the treatment that reached 100% germination was TOR-M16. Isolate CMR-M7 reached 97.49% mycelial growth percentage at 288 h, being the best result; in the percentage of mortality in CBB adults, the treatments that reached 100% were TOR-M9, TOR-M16, and MNR-M1. In general, the results demonstrate the entomopathogenic potential of native isolates of Metarhizium sp. acting as biocontrol agents of Hypothenemus hampei, being a low-cost, easily accessible, and environmentally friendly alternative.

Coffee is a crop of global importance, and it is especially important in countries such as Peru. However, the presence of the pest Hypothenemus hampei represents a significant challenge with a notable economic impact. This study addresses this challenge using entomopathogenic fungi such as Beauveria peruviensis and Metarhizium sp. The compatibility of three strains of Beauveria peruviensis (F5, P19, and P4) and seven strains of Metarhizium sp. (MMR-M1, LLM-M2, MHR-M4, PMR-M12, MMR-M15, TOR-M16, and GOR-M18) was evaluated for approximately 2 months. A total of 14 treatments were designed, each consisting of one strain of B. peruviensis and one strain of Metarhizium sp. The Skott–Knott test (p ≤ 0.05) revealed that strain LLM-M2 (Metarhizium sp. strain) had the highest conidial production (3.75 × 107 conidia/mL). Except for T6 (MMR-M1/F5), which showed a mutual growth type interaction (type A), all other strain combinations showed a type B interaction (mutual inhibition by contact or separation between colony margins (B. peruviensis and Metarhizium sp. as a biocontrol strategy against the coffee berry borer in the Amazon department, presenting them as an alternative to traditional chemical methods.

La degradación de los hábitats de especies endémicas con rangos de distribuciones restringidas, se han acelerado en los últimos años, aún más si se encuentran en peligro de extinción. En este estudio se evaluó el estado de las áreas ambientalmente sensibles a la degradación de los hábitats de dos especies de aves endémicas, Loddigesia mirabilis y Xenoglaux loweryi al noroeste de Perú. El análisis se basó en determinar un mapa de las áreas ambientalmente sensibles a la degradación de la tierra del área de estudio, utilizando el método Desertificación del Mediterráneo y uso de la tierra (MEDALUS) y como rangos de hábitat de las dos especies se superpusieron los mapas de distribución de la IUCN. El estado de las áreas ambientalmente sensibles a la degradación del hábitat de Loddigesia mirabilis estuvo representada el 78,40 % de la superficie en riesgo moderado, seguido por un alto riesgo (13,52 %), bajo (7,77 %), y grave (0,31%). Por otro lado, el estado de las áreas ambientalmente sensibles a la degradación del hábitat de Xenoglaux loweryi, se encontró que el 87,84 % estaba en riesgo moderado, seguido por un riesgo bajo (10,81 %), alto (1,35 %) y grave (0,01 %). Los resultados pueden contribuir como información de referencia para los tomadores de decisiones para la recuperación de hábitats a través de la conservación de ambas especies.

This research aimed to evaluate the oxidative stability and rheological properties of dark chocolates with the addition of essential oils (EO) of Cymbopogon citratus, Pimpinella anisum, and Mintostachys mollis. For this purpose, before the inclusion in chocolates, the EO were chemically characterized to identify the most important volatile compounds. We added essential oils of P. anisum, C. citratus and M. mollis to dark chocolates (cocoa 70%) at doses of 10, 12 and 14 μL per 500 g, separately. These chocolates were evaluated for oxidative activity, hardness, microstructure, rheological and melting properties and antioxidant capacity. It was found that C. citratus EO (10 μL/500 g of chocolate) improve the oxidative stability of the chocolates at 90 days of storage at 25 °C (230 meq O2/kg), while higher concentrations promote lipid oxidation. The incorporation of essential oils improves the antioxidant capacity, likewise, it changes the rheological, thermal, and microstructural properties. Therefore, essential oils can improve the physicochemical characteristics of dark chocolates allowing greater stability in oxidative fat and thus increase the shelf life.

One of the world’s major agricultural crops is rice (Oryza sativa), a staple food for more than half of the global population. In this research, synthetic aperture radar (SAR) and optical images are used to analyze the monthly dynamics of this crop in the lower Utcubamba river basin, Peru. In addition, this study addresses the need to obtain accurate and timely information on the areas under cultivation in order to calculate their agricultural production. To achieve this, SAR sensor and Sentinel-2 optical remote sensing images were integrated using computer technology, and the monthly dynamics of the rice crops were analyzed through mapping and geometric calculation of the surveyed areas. An algorithm was developed on the Google Earth Engine (GEE) virtual platform for the classification of the Sentinel-1 and Sentinel-2 images and a combination of both, the result of which was improved in ArcGIS Pro software version 3.0.1 using a spatial filter to reduce the “salt and pepper” effect. A total of 168 SAR images and 96 optical images were obtained, corrected, and classified using machine learning algorithms, achieving a monthly average accuracy of 96.4% and 0.951 with respect to the overall accuracy (OA) and Kappa Index (KI), respectively, in the year 2019. For the year 2020, the monthly averages were 94.4% for the OA and 0.922 for the KI. Thus, optical and SAR data offer excellent integration to address the information gaps between them, are of great importance to obtaining more robust products, and can be applied to improving agricultural production planning and management.

We report a disjunct population of Dicrodon guttulatum Duméril & Bibron, 1839 on the eastern slope of the Cordillera Occidental in the inter-Andean Seasonally Dry Forests of the Marañón River, in the Departments of Cajamarca and Piura in northwestern Peru. We include an updated range distribution map using records from museum specimens, the Global Biodiversity Information Facility, and available photographic records on iNaturalist. In addition, we identify widespread cultivation of rice crops as the main threat to D. guttulatum in the inter-Andean Seasonally Dry Forests of the Marañón.

Apiculture plays a vital role in maintaining a genetically diverse ecosystem and is an economic activity that contributes to the development of rural communities, thereby enhancing the livelihoods of beekeepers. However, despite the presence of over forty thousand beekeepers in Peru, there is currently no cartographic information available on optimal areas for the development of apiculture. Our study focused on assessing the suitability of land for apiculture development in rural and indigenous communities within the Amazonas Department in northwest Peru. We integrated biophysical and socioeconomic criteria using the Multiple Criteria Evaluation (MCE) technique, in conjunction with state-of-the-art geoinformation and earth observation techniques, to model and validate land suitability for supporting apiculture. It was identified that suitability is influenced by biophysical criteria (65%) and socioeconomic criteria (35%), resulting in highly suitable areas covering 315.6 km2 within the territory of peasant communities, 128.4 km2 within native communities, and an additional 41.4 km2 within conserved areas. Furthermore, to validate our results, we combined the use of high-resolution satellite imagery and visits to artisanal producers. This research provides valuable insights for spatiotemporal land use planning, emphasizing apicultural activity as a driver of rural development and biodiversity conservation. Consequently, this study contributes as a management tool to promote apicultural activities as support for rural development and in local-level decision making.

Coffee (Coffea arabica) is the main commodity in Peru and is the economic support for thousands of small farmers. However, coffee production is affected by the coffee berry borer (Hypothenemus hampei). Currently, H. hampei is the most important pest in whole coffee-growing regions in Peru. This study aimed to evaluate in vitro biological activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei in two trials at different times. Conidia production, Conidia viability, and pathogenicity against H. hampei were evaluated at three concentrations (1 × 105, 1 × 107, and 1 × 109 conidia/mL−1). In addition, lethal times (LT50 and LT90) and lethal concentrations (LC50 and LC90) were calculated. There were significant differences in conidia production (P

Cocoa (Theobroma cacao) is the main raw material for the production of chocolate; it is considered the food of the gods, as it possesses a diversity of bioactive compounds beneficial to human health. The abundance of bioactive compounds, among others, is conditioned by the post-harvest processing of cocoa beans, and fermentation is a major step in this regard. Consequently, this research evaluated the changes in phenolic compounds and methylxanthines occurred in the fermentation of Criollo and CCN-51 cocoa beans, varieties of great commercial interest for the cocoa-growing areas of Peru. For this purpose, samples were taken every 12 h of cocoa beans under fermentation for 204 h in which phenols (gallic acid, caffeic acid, catechin, and epicatechin) and methylxanthines (theobromine, caffeine and theophylline) were quantified by ultra-high performance liquid chromatography (UHPLC); total polyphenols by Folin Ciocalteu; antioxidant capacity by DPPH free radical capture method; total anthocyanins; pH; titratable acidity; and fermentation rate of beans. We found that during fermentation, phenolic content, antioxidant activity, and methylxanthines of cocoa beans decreased; on the other hand, the anthocyanin content increased slightly. Indeed, at distinctly degree, fermentation influences bioactive compounds in cocoa beans, depending on the variety cultivated.

Although the coffee berry borer (CBB) is the most important coffee pest worldwide, controversies remain regarding basic aspects of its behavior, such as how this is influenced by abiotic factors of the coffee agroecosystem. In this study, we compared the level of infestation and total population per fruit under three different levels of shade (full sun, up to 40%, and >40%), for two varieties of coffee (Caturra and Catimor) and at two different altitudes (1200 to 1700 m above sea level) in Rodríguez de Mendoza, Peru. We found that the infestation percentage increases with the shade levels of the plot. The average percentages of infestation according to shade levels were 10.52% for coffee in full sun, 12.56% with up to 40% shade, and 17.99% for coffee growing in more than 40% shade; however, maximum infestation values of 68.421%, 84.127%, and 95.238% were obtained for coffee plantations in full sun, up to 40% shade, and more than 40% shade, respectively. The Catimor variety was found to be more susceptible to CBB infestation than Caturra. In addition, CBB infestation per fruit was found to decrease with increasing altitude.

It has been established that climate change has a direct impact on water availability, an essential resource for agricultural development. As a result, controlling, mitigating, and adapting to water deficit requires the advancement of research on promising wild flora species. As recent studies have shown, wild relatives of certain cultivars are tolerant to adverse factors, enabling the development of sustainable and resilient agriculture. The present study evaluated the morpho-physiology and productivity of tomato scions grafted on wild Solanaceae (Datura stramonium, Solanum sisymbriifolium, Solanum quitoense, and Cyphomandra betacea) grown under water deficit conditions (100% ETc - high level, 75% ETc - moderate level, 50% ETc - medium level, and 25% ETc - low level). The results showed that tomato plants grafted on Datura stramonium rootstocks performed better morpho-physiologically under deficit irrigation. The improved osmoregulation caused by a higher relative water content (98.49%) allowed the scion to be more tolerant to water stress. In addition, these scions showed high water potential during their phenological stages (vegetative −0.47 MPa, flowering −0.59 MPa, and production −0.64 MPa), as well as improved photosynthetic efficiency. The overall tolerance of the scion resulted in better yield (8.14 kg/plant) with higher number of commercially valuable fruits. The D. stramonium rootstock allowed better management and use of irrigation water, increasing productivity (54.95 kg/m3); that is, it is presented as a species with potential for establishing tomato production areas in scenarios of water scarcity or cultivation under deficit irrigation.

Lakes are water bodies that play an essential role as water sources for humanity, as they provide a wide range of ecosystem services. Therefore, this study aimed to evaluate Lake Pomacochas, a high Andean lake in the north of Peru. A variety of parameters were studied, including physicochemical parameters such as temperature (T°C), dissolved oxygen (DO), potential hydrogen (pH), electrical conductivity (EC), turbidity, total dissolved solids (TDS), biochemical oxygen demand (BOD), alkalinity, and chlorides hardness; the concentrations of nitrates, nitrites, sulfates, and ammonium; elements such as aluminum (Al), calcium (Ca), magnesium (Mg), sodium (Na), potassium (K), and boron (B); as well as metals and metalloids such as zinc (Zn), cadmium (Cd), copper (Cu), lead (Pb), and arsenic (As). In addition, pH, Zn, and Cu were evaluated at the sediment level. It is important to note that all parameters evaluated in the water matrix showed significant differences in the seasonal period and depth levels. In comparison, the parameters evaluated at the sediment level had no significant differences between the seasonal period and sampling points. As for the seasonal period, the variables that were higher for the dry season were electrical conductivity, total dissolved solids, and lead while that for the wet season were biochemical oxygen demand, zinc, magnesium, turbidity, calcium, dissolved oxygen, temperature, and potential hydrogen. At the depth levels, parameters such as total dissolved solids, lead, and arsenic had similar behavior for the three depths evaluated. According to national standards, latent contamination by cadmium and lead was found in the lake water from the ecological risk assessment. However, by international standards, all sampling stations showed a high level of contamination by cadmium, lead, zinc, copper, and arsenic, which represents a potential risk for the development of socioeconomic activities in the lake. At the same time, the evaluation of sediments did not present any potential risk.

Due to the growing demand for healthy food products, the industry is seeking to incorporate inputs with high nutritional potential to traditional products. The objective of this research was to evaluate the effect of incorporating Lepidium meyenii, Chenopodium pallidicaule, Amaranthus caudatus, Sesamum indicum and Salvia hispanica flours on the physical, chemical, rheological, textural and thermal characteristics, and the degree of sensory acceptance of dark chocolate bars (65% cocoa). To this end, chocolate bars were made with the incorporation of five flours in four doses (1, 2, 3 and 4%), obtaining 20 different formulations compared with a control treatment (without flour addition). It was found that as flour incorporation levels increased, viscosity, antioxidants and particle size of the chocolates increased, but hardness and pH decreased. The addition of the flours also affected the acceptability and microstructure of the chocolate bars. The incorporation of up to 4% of the flours studied improved the degree of acceptance of the chocolates. Consequently, the incorporation of grain flours with high nutritional value can enhance the characteristics of dark chocolates, becoming a technological alternative for the chocolate industry.

Spectacled bears (SB) (Tremarctos ornatus) are the only bear species native to South America. This particular bear is the single species of its genus, and it is listed as vulnerable according to the IUCN red list. A critical SB conservation habitat is in the rural territories of the Peruvian Amazon, where anthropogenic land-use changes and landscape fragmentation threaten SB habitats. The following questions arise in this context: How much has land-use changed? How to design the establishment of ecological corridors (ECs) to support the conservation of SB?. We investigated the temporal land use and land cover changes for last 30 years (1990–2020) for a better projection of the ECs and to quantify the temporal landscape metrics. Furthermore, we integrated cloud computing, machine learning models with cost-effective techniques to delineate the ECs for SB within the rural territories. Ensemble Random Forest model associated with Google Earth Engine (GEE) was used to develop four land use and land cover (LULC) maps (for the years 1990, 2000, 2010 and 2020). The least cost path (LCP) model based on Dijkstra’s shortest path algorithm was assembled based on six variables (altitude; slope; distance to roads; distance to population centers; land use map; inventory map of SB). Then, we calculated the ECs based on the multidirectional origin-destination points, we found that forest patches increased by 57% between 1990 and 2020. Results showed statistically significant agreement (R2 = 0.47; p

Cacao (Theobroma cacao L.) is the basis of the lucrative confectionery industry with “fine or flavour” cocoa attracting higher prices due to desired sensory and quality profiles. The Amazonas Region (north Peru) has a designation of origin, Fine Aroma Cacao, based on sensory quality, productivity and morphological descriptors but its genetic structure and ancestry is underexplored. We genotyped 143 Fine Aroma Cacao trees from northern Peru (Bagua, Condorcanqui, Jaén, Mariscal Cáceres, and Utcubamba; mainly Amazonas Region), using 192 single nucleotide polymorphic markers. Identity, group, principal coordinate, phylogenetic and ancestry analyses were conducted. There were nine pairs of matched trees giving 134 unique samples. The only match within 1,838 reference cacao profiles was to a putative CCN 51 by a Condorcanqui sample. The “Peru Uniques” group was closest to Nacional and Amelonado-Nacional genetic clusters based on FST analysis. The provinces of Bagua and Utcubamba were genetically identical (Dest = 0.001; P = 0.285) but differed from Condorcanqui (Dest = 0.016–0.026; P = 0.001–0.006). Sixty-five (49%) and 39 (29%) of the Peru Uniques were mixed from three and four genetic clusters, respectively. There was a common and strong Nacional background with 104 individuals having at least 30% Nacional ancestry. The fine aroma of cacao from Northern Peru is likely due to the prevalent Nacional background with some contribution from Criollo. A core set of 53 trees was identified. These findings are used to support the continuance of the fine or flavour industry in Peru.

The presence of toxic metals such as aluminum is described as a factor that could lead to a significant decrease in crop productivity, particularly for the cultivation of acidophilic plants. In the present study, in vitro cultivation of pineapple was used as a model to evaluate the role of silver nanoparticles (0.005, 0.01, 0.025, 0.05, and 0.1 g L−1 Ag NPs) against the negative effects of aluminum (0, 100, 300, and 500 μM AlCl3). The results showed that the presence of 0.025 g L−1 Ag NPs stimulated a higher concentration of photosynthetic pigments “a”, “b”, “a + b” and carotenoids in treatments with high levels of AlCl3. The application of Ag NPs allowed better shoot formation, improved chlorophyll a/b, and total/carotenoid ratios, as well as better levels of proline biosynthesis in response to stress. The synergistic interaction of Ag NPs and AlCl3 increased the availability and assimilation of mineral elements (K, Ca, Mg, Fe, Cu, Mn, and Zn) while decreasing Al translocation. In conclusion, the implementation and validation of Ag NPs in agricultural fields would be revolutionary because they represent a novel alternative for overcoming the limitations imposed by the presence of Al.

The northeastern region of Peru is one of the centers of origin of cocoa due to the great diversity of this cultivar. The objective of this study is, therefore, to search for different genetic groups of 146 ecotypes of fine-aroma native cacao from the northeastern region of Peru, based on the morphological descriptors of pods, seeds, sensory, yield, and sampling altitude. The data were analyzed using multivariate statistics; a cluster analysis was performed with the numerical and categorical variables, followed by a principal component analysis (PCA) and the DGC (Di Rienzo, Guzmán y Casanoves) mean comparison test for the numerical data. Contingency tables and the multiple correspondence analysis (MCA) were performed for the categorical data. We differentiated 5 genetic groups; helpfully, sensory characteristics of the flowers and pod, size and weight of the seeds, and pod index were in fact crucial in separating the groups. The ecotypes of the groups labeled as “Indes” and “Bagüinos” reported the best sensory characteristics with high floral and fruity notes and with a good yield expressed in pod index (13.88 and 11.88, respectively). Furthermore, these ecotypes are found at medium and high altitudes, above 500 m a.s.l., a factor that enables them to express their sensory and yield attributes. On the contrary, the ecotypes known as “Toribianos” and “Cajas” report the highest pod indices (20.77 and 16.78, respectively), resulting in low productivity. In the future, the variability of the ecotypes found will help establish genetic improvement programs that contribute to the development of cocoa farming in general.

Water contamination comes from many different sources, including, among others, factories, sewage treatment plants, mining activities (heavy metals), food-processing waste, agriculture runoff, animal waste, disposal of personal care products, and household chemicals. Therefore, the reuse of wastewater has become a fundamental strategy for sustainable water management and maintaining environmental quality. In this sense, this research presents a simple and economic alternative to solving the problems caused by greywater, resulting from laundry activities. This paper evaluates a mixed system for the treatment of greywater. The mixed system has physical filters that are composed of river stone, concrete, river sand, and coal. A bioremediation technique is also evaluated, involving two types of aquatic plants, watercress (Nasturtium officinale L.) and duckweed (Lemma minor L.). This study showed significant differences in pH reduction, from 9.56 to 7.50, total suspended solids (TSS), from 1742.00 mg/L to 298.50 mg/L, phosphates, from 1.12 mg/L PO4 3¯ to 0.31 mg/L PO4 3- , and chemical oxygen demand (COD), from 472.38 mg/L to 8.52 mg/L. Thus, the results indicate that this system is efficient for the reuse of grey water for irrigation uses. Moreover, each parameter, with the exception of dissolved oxygen and total suspended solids (TSS), meets the maximum limits set by the Environmental Quality Standards for Category 3: irrigation water for vegetables and animal beverages and the FAO irrigation water standards.

Peru is one of the main producers of fine aroma native cacao, expanding its areas considerably in recent years, which makes it necessary to seek adequate management alternatives to obtain advantageous yields. The present work had the objective of testing the influence of Trichoderma sp. endophytic strains on the agronomic characteristics of ecotypes of Theobroma cacao L. under nursery conditions, Cajaruro district, Utcubamba, Amazonas, Peru. The Trichoderma strains evaluated were Trichoderma breve; T. harzianum; T. longibrachatum; T. afrojarzianum, and Trichoderma sp. which were inoculated on cocoa seedlings of ecotypes CCN51; TCHS565; and Nativo fino de aroma. The variables evaluated were chlorophyll indices (at 30, 45, 60, and 70 days after planting), percentage of endophyte colonization, root hair development, trichomes on stems, and agronomic characteristics (plant height, number of leaves, stem diameter, root dry and fresh weight, root size, etc.). The results showed that the application of Trichoderma breve in the fine aroma native cocoa ecotype (T12) presented the highest chlorophyll index at 75 days after planting (DAP) with 43.53 ± 1.59 and 49.77 ± 2.42 for the apex and leaf base, respectively, with a percentage of colonization in the root hairs of 66.67%, and with better characteristics for the number of leaves with 12.00 ± 3.46. T12 showed positive influences for plant height, leaf number, and chlorophyll index. Treatments based on T. harzianum + CCN51 (T5) and T. afroharzianum + TSHS565 (T6) showed 100% colonization of the root hairs and trichomes on stems. Endophytic Trichoderma fungi are an alternative for organic production of fine aroma cocoa in Peru, improving the agronomic characteristics of the crop.

Cocoa cultivation is of considerable economic and social importance to the Amazonas region and is commonly associated with forest species in the region. However, the diversification level and composition of cacao agroforestry systems in Peru are poorly understood. The objective of this study is, therefore, to describe the diversity of tree species in cocoa AFS by plantation age. Accordingly, the number of species of 15 plots covering a total of 1.5 hectares was recorded. Moderately low levels of tree species diversity were reported (H´ ranged 0.89–1.45). In total 17 species were reported throughout the study area. The most abundant botanical family was represented by a single Musa sp. species. The dissimilarity indices show a moderate similarity between the age ranges evaluated (over 62%). Additionally, the IVI indicates that the most important species are used for food and timber apart from providing shade, additionally major of this species are introduced intentionally for the farmers. Based on the observations, it may be concluded that the farmer’s interest in obtaining further benefits from the plot, mostly economic benefits affect the diversification of cocoa agroforestry systems.

Lilies are one of the most important, beautiful, and economically valuable flowers in the world. Lilium is regarded as a popular floral trade cut flower, so viable protocols are needed to provide seed production, multiplication, and preservation. In vitro protocols allow for rapid large-scale production and rejuvenation of planting material, but to be a commercially viable multiplication method, the procedure must allow for rapid production of viable, true-to-type plants quickly. The objective was to evaluate the in vitro production of microbulbs of five lily varieties (Lilium “Champion Diamond,” Lilium “Yellow Diamond,” Lilium “Batavus,” Lilium “Hyde Park,” and Lilium sp.) using different concentrations of 6-benzylaminopurine (0, 0.5, 1.0, 1.5, 1.5, and 2.0 mg L−1 BAP) and to determine the commercial quality (flowering) of the plants grown from the seed obtained. Results from the micropropagation phase show Lilium “Batavus” and Lilium “Hyde Park” varieties had better in vitro responses, especially when grown with 1.0 and 1.5 mg L−1 BAP, respectively. Plants (of all varieties) grown from microbulbs showed positive growth and generally resulted in commercially viable flower production. Finally, the results of this study support the use of bulb scales as an alternative for obtaining vegetative seeds with high potential for lily cultivation.

The evaluation of suitable landfill sites is a complex process and requires various legislative, technical, social, and environmental criteria. Therefore, this study provides a management tool for identifying suitable sites for landfills through the integrated use of the analytic hierarchy process (AHP), geographic information systems (GISs), and remote sensing (RS). Accordingly, fourteen subcriteria were identified and grouped into physical (7), environmental (3), and socioeconomic (4) criteria and were weighed using pairwise comparison matrices (PCMs). The weighted linear combination (WLC) approach of maps allowed us to generate models and submodels of land suitability. From the territory of the districts of Chachapoyas and Huancas, 0.9% (1.71 km2), 71.1% (141.89 km2), 21.0% (41.86 km2), 0.0%, and 7.7% (14.21 km2) have highly suitable, moderately suitable, marginally suitable, unsuitable, and restricted conditions, respectively, for a landfill site. Twelve highly suitable sites were identified, of which three were selected based on their shape and the minimum area required for the operation of the landfill until 2040. In fact, this study proposes a management tool for decision-makers (DMs) that improve the process of selecting landfill sites, supported by engineering and its applications for territorial sustainability.

En el año 2014, la Unión Europea impuso estándares máximos de tolerancia en cadmio, para la importación de productos a base de cacao, causando preocupación en los países. Se analizó estudios globales referidos a investigaciones en Theobroma cacao, relacionados con la actividad del cadmio en la atmósfera. Se utilizaron análisis bibliométricos en los programas R y VOSviewer, para examinar 64 documentos publicados en la base de datos Scopus según palabras clave. Se identificaron 811 palabras clave en coocurrencias de términos, 5 grupos temáticos en acoplamiento bibliográfico, 20 instituciones como afiliaciones más importantes, 20 países de procedencia de autores corresponsales, 112 instituciones en red de coautoría de los cuales 5 están en documentos primarios, y dos grupos en similaridad temática en co-citación de documentos. Estados Unidos lidera la producción científica con 11 documentos, seguido de Colombia (8) y Ecuador (7). En 1996 se registró el primer artículo científico para la red, con incrementos de hasta 11 publicaciones al 2020. En conclusión, se evidencia la necesidad de fortalecer y crear más redes de investigaciones entre países, instituciones, autores y coautores. Se espera que los resultados permitan desentrañar de manera integral la trayectoria de investigaciones cadmio-cacao, al tiempo que arrojen nuevas investigaciones prospectivas.

This work reports the formation of circular cavities and Au-SiOx nanoflowers after annealing of thin Au film deposited on SiO2/Si substrates, and the transformation of nanoflowers to nanosprouts with increasing the annealing time. Two reference experiments indicate that both H2 and Si are indispensable for the above structures. The formation of cavities can be attributed to the SiO2 layer decomposition and the product, volatile SiO, provides a Si source for the formation of nanoflowers at the early stage. A model is proposed to indicate that SiO gas produced at the Si/SiO2 interface can diffuse to the surface assisted by the defects in the SiO2 layer before the decomposed cavities are exposed. Then the exposing of those cavities introduces another volatile SiO from the active oxidation of Si substrate, provoking a change in the direction of the main Si source, which in turn makes the one nanoparticle of the nanoflower split in two and finally form the nanosprout. The model about the escape of SiO further details SiO2 decomposition process, and the transformation mechanism from nanoflowers to nanosprout sheds light on a feasible nanofabrication method to design tunable size and shape of nanoparticles.

Las Áreas de Conservación Privada (ACP) son uno de los mecanismos de conservación, gestionadas por ciudadanos privados que más protagonismo han adquirido en los escenarios de conservación local en los últimos años. En este estudio evaluamos la efectividad de cuatro ACP gestionadas por comunidades locales (CC). Se aplicó el Índice de Efectividad Compuesto (IEC) para determinar la efectividad del diseño, la integridad ecológica y la gestión. Los resultados muestran sistemas de gestión con una efectividad media, tres de las cuatro ACP evaluados (Copallín, Huaylla Belén-Colcamar y Tilacancha) reportan un diseño efectivo. Los rangos altitudinales protegidos están entre 2500 y 3500 m.s.n.m., con un índice de representatividad de la superficie promedio de 4,55% con respecto al área conservada en la categoría ACP para el departamento de Amazonas. La evaluación de la integridad ecológica indica que las ACP presentan menor superficie transformada (TS) (0-10%) y mayor TS en sus áreas circundantes, especialmente en el ACP Tilacancha (13,37% de TS en un buffer de 1,5 km). La suma ponderada de los índices individuales resulta en índices de efectividad compuestos de mayor a menor para el ACP Copallín (2,22), Hierba Buena Allpayacku (1,82), Huaylla Belen Colcamar (1,81) y Tilacancha (1,56).

Introducción. Las propiedades nutricionales, capacidad antioxidante y valor comercial de la pitahaya amarilla son un atractivo para su siembra, por ello, es necesario estudiar mejoras tecnológicas para su propagación. Objetivo. Evaluar la viabilidad del establecimiento y multiplicación in vitro de pitahaya amarilla, con el uso como explantes de plántulas provenientes de la germinación in vitro y distintas fuentes hormonales. Materiales y métodos. El experimento se desarrolló en el Laboratorio de Fisiología y Biotecnología Vegetal de la Universidad Nacional Toribio Rodríguez de Mendoza, Perú, entre febrero y junio de 2020. Se evaluaron cuatro tratamientos para la germinación [control: agua estéril (3 mL), E1: MS (50 %), sacarosa (30 g L-1), agar (6 g L-1), E2: AG3 (250 mg L-1), E3: MS (100 %), sacarosa (30 g L-1), agar (6 g L-1), AG3 (250 mg L-1)] y multiplicación [control: MS (100 %), sacarosa (30 g L-1), agar (7 g L-1), M1: MS (100 %), sacarosa (30 g L-1), agar (7 g L-1), agua de coco (10 ml L-1), M2: MS (100 %), sacarosa (30 g L-1), agar (7 g L-1), agua de coco (20 ml L-1), carbón activado (2 g L-1), M3: MS (100 %), sacarosa (30 g L-1), agar (7 g L-1), BAP (0,10 mg L-1), ANA (3 mg L-1)]. Las variables evaluadas fueron: germinación (%), número y longitud de brotes y raíces, formación de callos (%), enraizamiento (%) y número de aréolas. Resultados. La mejor germinación (100 %) se obtuvo en el medio E1, con más de tres raíces y dos brotes por plántula. El medio M2 generó la mejor multiplicación, con 82,5 % de enraizamiento, brotes de 1,66 cm y 1,86 aréolas, y menor presencia de tejido calloso (60 %). Conclusión. Se logró la micropropagación de pitahaya amarilla al emplear como fuente de explante plántulas obtenidas in vitro.

Unmanned Aircraft Systems (UAS) are used in a variety of applications with the aim of mapping detailed surfaces from the air. Despite the high level of map automation achieved today, there are still challenges in the accuracy of georeferencing that can limit both the speed and the efficiency in mapping urban areas. However, the integration of topographic grade Global Navigation Satellite System (GNSS) receivers on UAS has improved this phase, leading to a reach of up to a centimeter-level accuracy. It is therefore necessary to adopt direct georeferencing (DG), real-time kinematic positioning (RTK)/post-processed kinematic (PPK) approaches in order to largely automate the photogrammetric flow. This work analyses the positional accuracy using Ground Control Points (GCP) and the repeatability and reproducibility of photogrammetric products (Digital Surface Model and ortho-mosaic) of a commercial multi-rotor system equipped with a GNSS receiver in an urban environment with a DG approach. It was demonstrated that DG is a viable solution for mapping urban areas. Indeed, PPK with at least 1 GCP considerably improves the RMSE (x: 0.039 m, y: 0.012 m, and z: 0.034 m), allowing for a reliable 1:500 scale urban mapping in less time when compared to conventional topographic surveys.

Peru is one of the world’s leading exporters of cocoa beans, which directly impacts the household economy of millions of small farmers. Currently, the expansion and modernization of the cocoa-growing area require the zoning of the territory with suitable biophysical and infrastructural conditions to facilitate optimizing productivity factors. Therefore, we analyzed land suitability for cocoa (Theobroma cacao L.) production on the Peruvian mainland as a support measure for sustainable agriculture. To this end, the climatological, edaphological, orographic, and socioeconomic criteria determining sustainable cocoa cultivation were identified and mapped. Three modeling approaches (Analytic Hierarchy Process—AHP, Maximum Entropy—MaxEnt, and AHP—MaxEnt combined) were further used to hierarchize the importance of the criteria and to model the potential territory for sustainable cocoa cultivation. In all three modeling approaches, climatological criteria stood out among the five most important criteria. Elevation (orographic criteria) is also featured in this group. On the other hand, San Martin and Amazonas emerged as the five regions with the largest area ‘Highly suitable’ for cocoa cultivation in all three modeling approaches, followed by Loreto, Ucayali, Madre de Dios, Cusco, Junín, and Puno, which alternated according to modeling approach. From most to least restrictive, the AHP, MaxEnt, and AHP–MaxEnt modeling approaches indicate that 1.5%, 5.3%, and 23.0% of the Peruvian territory is ‘Highly suitable’ for cocoa cultivation, respectively.

Amazonas is a mountain region in Peru with high cloud cover, so using optical data in the analysis of surface changes of water bodies (such as the Burlan and Pomacochas lakes in Peru) is difficult, on the other hand, SAR images are suitable for the extraction of water bodies and delineation of contours. Therefore, in this research, to determine the surface changes of Burlan and Pomacochas lakes, we used Sentinel-1 A/B products to analyse the dynamics from 2014 to 2020, in addition to evaluating the procedure we performed a photogrammetric flight and compared the shapes and geometric attributes from each lake. For this, in Google Earth Engine (GEE), we processed 517 SAR images for each lake using the following algorithms: a classification and regression tree (CART), Random Forest (RF) and support vector machine (SVM).) 2021-02-10, then; the same value was validated by comparing the area and perimeter values obtained from a photogrammetric flight, and the classification of a SAR image of the same date. During the first months of the year, there were slight increases in the area and perimeter of each lake, influenced by the increase in rainfall in the area. CART and Random Forest obtained better results for image classification, and for regression analysis, Support Vector Regression (SVR) and Random Forest Regression (RFR) were a better fit to the data (higher R2), for Burlan and Pomacochas lakes, respectively. The shape of the lakes obtained by classification was similar to that of the photogrammetric flight. For 2021-02-10, for Burlan Lake, all 3 classifiers had area values between 42.48 and 43.53, RFR 44.47 and RPAS 45.63 hectares. For Pomacohas Lake, the 3 classifiers had area values between 414.23 and 434.89, SVR 411.89 and RPAS 429.09 hectares. Ultimately, we seek to provide a rapid methodology to classify SAR images into two categories and thus obtain the shape of water bodies and analyze their changes over short periods. A methodological scheme is also provided to perform a regression analysis in GC using five methods that can be replicated in different thematic areas.

The objective of this study was to evaluate the effect of indole-3-butyric acid (IBA) on the rooting of five wild accessions of blueberry (HCHA-262, HCHA-271, HCHA-283, HCHA-286, and HCHA-290) obtained from natural populations in the province of Chachapoyas (northern Peru). Buds were collected from the middle third of the plant and treated with IBA at concentrations of 0, 1,000, 2,000, and 3,000 mg L−1. The results indicated that of the IBA doses, the 2,000-mg L−1 dose achieved the best effects on the rooting percentage and the lengths and numbers of roots and shoots of each accession studied. Accessions HCHA-262 and HCHA-283 showed greater regeneration of new seedlings than the other accessions after 45 days. The rhizogenic capacity of the cuttings varied because of the influences of both the IBA concentration and the genotype of the mother plant.

Cocoa beans are the raw material for the chocolate industry. In this study, the total fat contents and fatty acid profiles of fine-aroma cocoa beans of 30 cocoa ecotypes from northeastern Peru were evaluated. Results showed that SJJ-1 and ACJ-11 ecotypes from San Martin and Amazonas regions, respectively, presented highest percentages of total fat with an average of 30.49%. With respect to fatty acid profiles, it was found that cocoa ecotypes are composed of 10 fatty acids (C14:0, C16:0, C16:1, C18:0, C17:0, C18:1, C18:2, C18:3, C20:0, and C22:0); based on this profile, 5 clusters were determined. Cluster 5 had the highest content of C17:0 fatty acid (0.47%); however, the clusters 1, 2, 3, and 4 had the lowest content of this fatty acid (0.37%, 0.32%, 0.32%, respectively). The clusters 3 and 4 showed the highest content of C16:0 fatty acid (31.13% y 28.97%, respectively). The clusters 3 and 5 contained the highest content of the acid C18:1 (27.08% y 26.82%, respectively). The PCA found that C18:0 and C20:0 fatty acids are correlated, and are fundamentally opposite to C18:1, C16:0, and C18:3 acids. These results may be useful in identifying raw material for the development of specialty chocolates with better nutritional value than traditional cocoa.

The genus Beauveria is considered a cosmopolitan anamorphic and teleomorphic genus of soilborne necrotrophic arthropod-pathogenic fungi that includes ecologically and economically important species. Species identification in Beauveria is difficult because of its structural simplicity and the lack of distinctive phenotypic variation. Therefore, the use of multi-locus sequence data is essential to establish robust species boundaries in addition to DNA-based species delimitation methods using genetic distance, coalescent, and genealogical concordance approaches (polyphasic approaches). In this regard, our study used multilocus phylogeny and five DNA-based methods to delimit species in Beauveria using three molecular makers. These polyphasic analyses allowed for the delimitation of 20–28 species in Beauveria, confirming cryptic diversity in five species (i.e. B. amorpha, B. bassiana, B. diapheromeriphila, and B. pseudobassiana) and supporting the description of B. peruviensis as a new taxon from northeastern Peru. The other five species were not evaluated as they did not have enough data (i.e. B. araneola, B. gryllotalpidicola, B. loeiensis, B. medogensis, and B. rudraprayagi). Our results demonstrate that the congruence among different methods in a polyphasic approach (e.g. genetic distance and coalescence methods) is more likely to show reliably supported species boundaries. Among the methods applied in this study, genetic distance, coalescent approaches, and multilocus phylogeny are crucial when establishing species boundaries in Beauveria.