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The purpose of this study was to examine the involvement of early childhood pre-service students (PSSs) in children's constructive play and highlight how this involvement supported children's play skills towards advanced play behaviours as seen by previous literature. Drawing from the conceptual framework of the Zone of Proximal Development, as proposed by Vygotsky, and supporting adult involvement in children's learning, we specifically examine PSSs involvement in constructive play. Participants were four out of 26 PSSs participating in their school experience program phase III and 17 children of the ages 5-6 years. Data was collected through videos, 12 in total, of approximately 20 minutes each, during which the PSSs interacted with children while participating in constructive play. Also, data was collected through reflective journals reported after each video. Data analysis highlighted the different ways the PSSs employed to get involved in children's play, these being either using direct or indirect involvement. This study suggests that early childhood teachers' reflection can create a Zone of Proximal Action which refers to good fit direct and indirect involvement supporting children's mature constructive play behaviours.

OBJECTIVE: To evaluate the reliability, responsiveness, and validity of the Scale for the Assessment and Rating of Ataxia (SARA) in patients with lysosomal storage disorders (LSDs) who present with neurological symptoms, and quantify the threshold for a clinically meaningful change. METHODS: We analyzed data from three clinical trial cohorts (IB1001-201, IB1001-202, and IB1001-301) of patients with Niemann-Pick disease type C (NPC) and GM2 Gangliosidoses (Tay-Sachs and Sandhoff disease) comprising 122 patients and 703 visits. Reproducibility was described as re-test reliability between repeat baseline visits or baseline and post-treatment washout visits. Responsiveness was determined in relation to the Investigators, Caregivers, and Patients Clinical Global Impression of Improvement (CGI-I). The CGI-I data was also used to quantify a threshold for a clinically meaningful improvement on the SARA scale. Using a qualitative methods approach, patient/caregiver interviews from the IB1001-301 trial were further used to assess a threshold of meaningful change as well as the breadth of neurological signs and symptoms captured and evaluated by the SARA scale. RESULTS: The Inter-Class Correlation (ICC) was 0.95 or greater for all three trials, indicating a high internal consistency/reliability. The mean change in SARA between repeat baseline and post-treatment washout visit assessments in all trials was -0.05, SD 1.98, i.e., minimal, indicating no significant differences, learning effects or other systematic biases. For the CGI-I responses and change in SARA scores, Area Under the Curve (AUC) values were 0.82, 0.71, and 0.77 for the Investigators, Caregivers, and Patients CGI-I respectively, indicating strong agreement. Further qualitative analyses of the patient/caregiver interviews demonstrated a 1-point or greater change on SARA to be a clinically meaningful improvement which is directly relevant to the patients everyday functioning and quality of life. Changes captured by the SARA were also paralleled by improvement in a broad range of neurological signs and symptoms and beyond cerebellar ataxia. CONCLUSION: Qualitative and quantitative data demonstrate the reliability and responsiveness of the SARA score as a valid measure of neurological signs and symptoms in LSDs with CNS involvement, such as NPC and GM2 Gangliosidoses. A 1-point change represents a clinically meaningful transition reflecting the gain or loss of complex function.

Economical production of lignin-based jet fuel (LJF) can improve the sustainability of sustainable aviation fuels (SAFs) as well as can reduce the overall greenhouse gas emissions. However, the challenge lies in converting technical lignin polymer from biorefinery directly to jet fuel in a continuous operation. In this work, we demonstrate a simultaneous depolymerization and hydrodeoxygenation (SDHDO) process to produce lignin-based jet fuel from the alkali corn stover lignin (ACSL) using engineered Ru-HY-60-MI catalyst in a continuous flow reactor, for the first time. The maximum carbon yield of LJF of 17.9 wt% was obtained, and it comprised of 60.2 wt% monocycloalkanes, and 21.6 wt% polycycloalkanes. Catalyst characterization of Ru-HY-60-MI suggested there was no significant change in HY zeolite structure and its crystallinity after catalyst engineering. Catalyst characterizations performed post the SDHDO experiments indicate presence of carbon and K content in the catalyst. K content presence in the spent catalyst was due to K+ ion was exchanged between lignin solution and HY-60 while carbon presence validated the SDHDO chemistry on the catalyst surface. Tier α fuel property testing indicates that LJF production using SDHDO chemistry can produce SAF with high compatibility, good sealing properties, low emissions, and high energy density for aircraft.

The weight is a fundamental concept in physics. Its early handling in everyday life induces enormous modelling difficulties in learners, and these persist even after teaching. In the present study, we set out to determine the conceptions of Cameroonian pupils in the third year of general secondary education (aged 13-15) about the concept of the weight. Successive exploration of two avenues: an institutional avenue (analysis of the official textbook) and an epistemological avenue enabled us to construct a questionnaire consisting of 11 open and semi-open questions. It was administered to 95 learners in third-year classes who had been taught about the weight a year earlier. The data collected were analysed according to the modelling scheme proposed by Martinand (1996). The results showed that even after instruction, most of the learners still had an Aristotelian conception of the fall of bodies to earth. They still confuse the concepts of weight and mass and have difficulty representing the weight in a spatial reference frame. Learners' reasoning is therefore based almost entirely on phenomenography (Martinand, 1996). For a better conceptualisation of the weight concept, teachers should use the conceptions identified in this work to propose activities in which learners construct weight from the interpretation of everyday phenomena.

Salmonella spp. are one of the leading causes of gastroenteritis worldwide. Salmonella Heidelberg is an emergent pathogen associated with multidrug resistant outbreaks linked to poultry products. Its high persistence in the environment may be associated with its ability to adhere to different surfaces and to form biofilms. Owing to the increased antimicrobial resistance worldwide, researches have investigated the use of lactic acid bacteria (LAB) as biological control of pathogenic microorganisms. The aim of this study was to evaluate the ability of LABs for the control of S. Heidelberg biofilms on polystyrene surfaces. The antibiofilm activity of nine LAB, all belonging to Lactobacillus genera, in the inhibition of biofilm produced by S. Heidelberg was evaluated in vitro. Biofilm formation occurred only when LAB1 (Lactobacillus salivaris) was used. All other treatments have demonstrated antibiofilm activity. However, LAB were not capable to reduce bacterial count. Our results show that LAB is capable to avoid or to delay biofilm formation by S. Heidelberg on polystyrene surface and may be used for in vivo studies as a potential alternative to help in the control of this pathogen in the food industry.

Aims: We aimed to evaluate lesson learnt from a pilot project in Mozambique focused on point-of-care (POC) HbA1c testing for diabetes management in primary health care facilities. Methods: Over a three-year period, several health centers were equipped with POC HbA1c testing machines. The evaluation involved 12 months of data collection, interviews with patients and staff, and regular supervision visits. Results: The project screened over 22,000 individuals and provided HbA1c testing to 2362 diabetes patients. Among the analyzed results, 65.7 % had HbA1c levels below 7 %, 10.9 % between 7 % and 8.5 %, and 23.4 % above 8.5 %. POC testing showed advantages such as reduced costs and improved workload management. Conclusions: Limited access to HbA1c testing remains a challenge in African countries. The pilot project demonstrated the feasibility of POC HbA1c testing and highlighted the need for increased efforts to make it more widely available, leading to improved diabetes management and patient outcomes.