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10 results on '"Mant'eva VL"'

1. [Isolation and comparative characteristics of the DNA attached to the axial structures of interphase nuclei and metaphase chromosomes].

Author

Razin SV, Mant'eva VL, and Georgiev GP

Subjects
Animals, Base Composition, Base Sequence, DNA Restriction Enzymes, Interphase, L Cells, Metaphase, Micrococcal Nuclease, Molecular Weight, Cell Nucleus analysis, Chromosomes analysis, DNA analysis
Abstract

DNA fractions enriched in sequences located closely to the sites of attachment of DNA loops to the axial protein structures of interphase nuclei ad metaphase chromosomes were isolated with the aid of two techniques. The method involving treatment with EcorI endonuclease has been described earlier. The new method is based on the mild treatment of the preparations with micrococcal nuclease. The latter method allows one to obtain the fraction of attached DNA of different length and to compare their properties. The average distance between two attachment sites was measured. It is equal to about 6.10(4) base pairs in the both interphase nuclei and metaphase chromosomes. The attached DNA obtained with the aid of micrococcal nuclease treatment is not enriched in satellite but contains a high amount of middle repetitive sequences renaturing in the Cot interval from 10(-2) to 10(0). According to renaturation properties and distribution in CsCl density gradient the fractions of attached DNA of similar size from metaphase chromosomes and from interphase nuclei are virtually identical. The data obtained indicate that the DNA sequences involved in the attachment of DNA loops to the matrix of interphase nucleus are the same as in the case of metaphase chromosomes.

Published
1980

2. [Isolation of DNA fraction bound to the axial structure of metaphase chromosomes and studies of renaturation].

Author

Mant'eva VL, Razin SV, and Georgiev GP

Subjects
Animals, Base Sequence, DNA Restriction Enzymes, Kinetics, L Cells cytology, Mice, Nucleic Acid Renaturation, Cell Cycle, Chromosomes analysis, DNA isolation & purification, Metaphase
Abstract

Mitotic chromosomes of L cells (metaphase plates) were dehistonized by centrifugation through a layer of 2 M NaCl and then treated with restriction endonuclease EcoRI and HindIII. Alternatively, they were pretreated with EcoRI endonuclease. The DNA remaining attached to the axial structure of the chromosomes was isolated and investigated in renaturation experiments. It was found to be enriched in reiterated base sequences belonging to the satellite and to abundant intermediate repeats.

Published
1979

4. The structure of nuclear D-RNA. I. The nature of the conversions of newly formed D-RNA under the action of an actinomycin block.

Author

Mant'eva VL, Avakyan ER, and Georgiev GP

Subjects
Animals, Carbon Isotopes, Carcinoma, Ehrlich Tumor, Cell Fractionation, Cell Nucleus analysis, Centrifugation, Density Gradient, DNA, Hot Temperature, Liver analysis, Methods, Mice, Nucleic Acid Hybridization, Orotic Acid metabolism, RNA isolation & purification, RNA, Messenger isolation & purification, RNA, Neoplasm, Rats, Dactinomycin pharmacology, RNA metabolism, RNA, Messenger metabolism
Published
1971

7. [The effect of x-irradiation on RNA biosynthesis in rat liver and in mouse Ehrlich ascites carcinoma].

Author

Mant'eva VL, Rapoport EA, Tul'kes SG, and Zbarskiĭ IB

Subjects
Animals, Cell Nucleolus metabolism, Cell Nucleolus radiation effects, Chromosomes metabolism, Chromosomes radiation effects, Cytoplasm metabolism, Cytoplasm radiation effects, DNA biosynthesis, DNA radiation effects, DNA, Neoplasm biosynthesis, DNA, Neoplasm radiation effects, Liver radiation effects, Mice, RNA radiation effects, RNA, Neoplasm radiation effects, RNA, Transfer radiation effects, Rats, Carcinoma, Ehrlich Tumor metabolism, Liver metabolism, RNA biosynthesis, RNA, Neoplasm biosynthesis, RNA, Transfer biosynthesis, Radiation Effects
Published
1966

8. The effect of cordycepin on the synthesis of nuclear and cytoplasmic D-RNA in mouse liver cells and Ehrlich's carcinoma cells.

Author

Podobed OV, Leitin VL, Brykina EV, Mant'eva VL, and Lerman MI

Subjects
Animals, Carbon Radioisotopes, Carcinoma, Ehrlich Tumor pathology, Cell Nucleus drug effects, Cell Nucleus metabolism, Centrifugation, Density Gradient, Cytosol drug effects, Cytosol metabolism, Liver cytology, Liver drug effects, Lysosomes metabolism, Male, Mice, Mice, Inbred BALB C, RNA, Messenger biosynthesis, RNA, Ribosomal biosynthesis, Ribosomes metabolism, Spectrophotometry, Ultraviolet, Uridine, Carcinoma, Ehrlich Tumor metabolism, Deoxyadenosines pharmacology, Liver metabolism, RNA biosynthesis, RNA, Neoplasm biosynthesis
Published
1973

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