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4. Fistules perilymphatiques post-traumatiques a propos de 13 cas

Author

Khamassi, K, Lahiani, R, Jlassi, N, Boulakbeche, R, Hassan, CH, Mannoubi, S, Ben Salah, M, Kaffel, N, and Ferjaoui, M

Subjects
fistule périlymphatique, traumatisme, vertige, surdité, tomodensitométrie, chirurgie
Abstract

Introduction : La fistule péri-lymphatique (fPL) post-traumatique réalise une communication anormale entre le compartiment liquidien périlymphatique et l’oreille moyenne. Son diagnostic est souvent difficile du fait du tableau clinique parfois incomplet. L’imagerie est indispensable afin d’étayer le diagnostic. Le traitement repose sur la chirurgie.Patients et méthode : : il s’agit d’une étude rétrospective à propos de 13 patients colligés sur une période de 13 ans (1996-2008). un examen ORL complet, une audiométrie et une imagerie (tomodensitométrie des rochers) ont été réalisés dans tous les cas. Tous les patients ont bénéficié d’un traitement médical et ont été opérés sous anesthésie générale. Le contrôle ultérieur était clinique, audiométrique et radiologique avec un recul moyen de 22 mois.Résultats :L’âge moyen était de 29 ans (20-47 ans) et le sex-ratio 3,33. Les signes fonctionnels étaient essentiellement représentés par l’hypoacousie dans 12 cas (92,3%), les vertiges dans 11 cas (84,6%) et les acouphènes dans 4 cas (30,8%). L’écoulement d’un liquide eau de roche par l’oreille a été rapporté par 2 patients (15,4%). L’examen clinique trouvait un nystagmus spontané battant du côté controlatéral au traumatisme chez un seul patient, et un signe de la fistule positif chez 2 patients (15,4%). A l’audiométrie, 9 patients (69,3%) avaient une surdité de perception ou mixte dont 6 cas de cophose. une surdité de transmission a été objectivée dans 3 cas. un patient avait une audition subnormale. La TdM des rochers a montré une fracture extra-labyrinthique dans 5 cas (38,5%) et une fracture trans-labyrinthique ou mixte dans 6 cas (46,2%). un pneumolabyrinthe a été constaté chez 5 patients (38,5%). une exploration chirurgicale a été indiquée chez tous les patients. En per-opératoire, une fPL a été mise en évidence dans tous les cas. Le siège de la fistule était au niveau du promontoire et/ou au niveau des fenêtres. un colmatage de la fistule a été réalisé dans tous les cas. Les matériaux utilisés étaient essentiellement de l’aponévrose temporale superficielle, du périchondre tragal et de la graisse. En post-opératoire, les vertiges se sont améliorés chez patients parmi les 11 vertigineux (81,8%). L’audition était améliorée chez 2 patients parmi les 9 ayant une surdité de perception ou mixte (18,2%).Conclusion : La fistule périlymphatique post-traumatique est de diagnostic difficile. Celui-ci est étayé par un faisceau d’arguments cliniques et paracliniques et n’est confirmé qu’en per-opératoire. L’indication d’une exploration chirurgicale et son délai dépendent essentiellement de la symptomatologie clinique et de son évolution dans le temps.Mots clés : fistule périlymphatique, traumatisme, vertige, surdité, tomodensitométrie, chirurgieObjective : Post-traumatic perilymphatic fistula (PLf) realises an abnormal communication between the perilymphatic fluid compartment and the middle ear. its diagnosis is often difficult because the clinical presentation is sometimes incomplete. imaging is essential in order to support the diagnosis. Treatment is based on surgery.Patients and methods : We carry a retrospective study about 13 patients collected over a period of 13 years (1996- 2008). A complete ENT examination, audiometry and imaging (petrous CT scan) were performed in all cases. All patients received medical treatment and were operated under general anaesthesia. Subsequent control was clinical, radiological and audiometric with a mean period of 22 months.Mean age was 29 years (20-47 years) and sex ratio 3.33. Symptoms were mainly hearing loss in 12 cases (92.3%), vertigo in 11 cases (84.6%) and tinnitus in 4 cases (30.8%). flow of liquid from the ear was reported by 2 patients (15.4%). Clinical examination showed spontaneous nystagmus beating to the contralateral side of the trauma in one patient, and a positive sign of the fistula in 2 patients (15.4%). At audiometry, 9 patients (69.3%) had sensorineural or mixed deafness, of which 6 cases of cophosis. Transmission deafness was found in 3 cases. One patient had a subnormal hearing. Petrous CT scan showed extra-labyrinthic fracture in 5 cases (38.5%) and trans-labyrinthic or mixed fracture in 6 cases (46.2%). A pneumolabyrinth was found in 5 patients (38.5%). Surgical exploration was indicated in all patients. PLf was found in all cases. The localization of the fistula was at the promontory and / or at the windows. Blockage of the fistula was achieved in all cases. Materials used were essentially superficial temporal fascia, tragal perichondrium and fat. Postoperatively, vertigo improved in 9 patients among 11 (81.8%). Hearing was improved in 2 patients among the 9 having sensorineural or mixed deafness (18.2%).Conclusion: TPost-traumatic perilymphatic fistula trauma is difficult to diagnose. This is supported by a beam of clinical and paraclinical arguments, and is confirmed intraoperatively. The indication for surgical exploration and its delay depend mainly on clinical symptomatology and its evolution over time.Keywords : perilymphatic fistula, trauma, vertigo, deafness, CT scan, surgery

Published
2015

6. Abstracts of the 40th National Congress of Medicine Tunis, 19-20 October 2017

Author

Abdallah, M., Abdelaziz, A., Abdelaziz, O., Abdelhedi, N., Abdelkbir, A., Abdelkefi, M., Abdelmoula, L., Abdennacir, S., Abdennadher, M., Abidi, H., Abir Hakiri, A., Abou El Makarim, S., Abouda, M., Achour, W., Aichaouia, C., Aissa, A., Aissa, Y., Aissi, W., Ajroudi, M., Allouche, E., Aloui, H., Aloui, D., Amdouni, F., Ammar, Y., Ammara, Y., Ammari, S., Ammous, A., Amous, A., Amri, A., Amri, M., Amri, R., Annabi, H., Antit, S., Aouadi, S., Arfaoui, A., Assadi, A., Attia, L., Attia, M., Ayadi, I., Ayadi Dahmane, I., Ayari, A., Azzabi, S., Azzouz, H., B Mefteh, N., B Salah, C., Baccar, H., Bachali, A., Bahlouli, M., Bahri, G., Baïli, H., Bani, M., Bani, W., Bani, M. A., Bassalah, E., Bawandi, R., Bayar, M., Bchir, N., Bechraoui, R., Béji, M., Beji, R., Bel Haj Yahia, D., Belakhel, S., Belfkih, H., Belgacem, O., Belgacem, N., Belhadj, A., Beltaief, N., Ben Abbes, M., Ben Abdelaziz, A., Ben Ahmed, I., Ben Aissia, N., Ben Ali, M., Ben Ammar, H., Ben Ammou, B., Ben Amor, A., Ben Amor, M., Benatta, M., Ben Ayed, N., Ben Ayoub, W., Ben Charrada, N., Ben Cheikh, M., Ben Dahmen, F., Ben Dhia, M., Ben Fadhel, S., Ben Farhat, L., Ben Fredj Ismail, F., Ben Hamida, E., Ben Hamida Nouaili, E., Ben Hammamia, M., Ben Hamouda, A., Ben Hassine, L., Ben Hassouna, A., Ben Hasssen, A., Ben Hlima, M., Ben Kaab, B., Ben Mami, N., Ben Mbarka, F., Ben Mefteh, N., Ben Kahla, N., Ben Mrad, M., Ben Mustapha, N., Ben Nacer, M., Ben Neticha, K., Ben Othmen, E., Ben Rhouma, S., Ben Rhouma, M., Ben Saadi, S., Ben Safta, A., Ben Safta, Z., Ben Salah, C., Ben Salah, N., Ben Sassi, S., Ben Sassi, J., Ben Tekaya, S., Ben Temime, R., Ben Tkhayat, A., Ben Tmim, R., Ben Yahmed, Y., Ben Youssef, S., Ben Atta, M., Ben Salah, M., Berrahal, I., Besbes, G., Bezdah, L., Bezzine, A., Bokal, Z., Borsali, R., Bouasker, I., Boubaker, J., Bouchekoua, M., Bouden, F., Boudiche, S., Boukhris, I., Bouomrani, S., Bouraoui, S., Bourgou, S., Boussabeh, E., Bouzaidi, K., Chaker, K., Chaker, L., Chaker, A., Chaker, F., Chaouech, N., Charfi, M., Charfi, M. R., Charfi, F., Chatti, L., Chebbi, F., Chebbi, W., Cheikh, R., Cheikhrouhou, S., Chekir, J., Chelbi, E., Chelly, I., Chelly, B., Chemakh, M., Chenik, S., Cheour, M., Cherif, E., Cherif, Y., Cherif, W., Cherni, R., Chetoui, A., Chihaoui, M., Chiraz Aichaouia, C., Dabousii, S., Daghfous, A., Daib, A., Daib, N., Damak, R., Daoud, N., Daoud, Z., Daoued, N., Debbabi, H., Demni, W., Denguir, R., Derbel, S., Derbel, B., Dghaies, S., Dhaouadi, S., Dhilel, I., Dimassi, K., Dougaz, A., Dougaz, W., Douik, H., Douik El Gharbi, L., Dziri, C., El Aoud, S., El Hechmi, Z., El Heni, A., Elaoud, S., Elfeleh, E., Ellini, S., Ellouz, F., Elmoez Ben, O., Ennaifer, R., Ennaifer, S., Essid, M., Fadhloun, N., Farhat, M., Fekih, M., Fourati, M., Fteriche, F., G Hali, O., Galai, S., Gara, S., Garali, G., Garbouge, W., Garbouj, W., Ghali, O., Ghali, F., Gharbi, E., Gharbi, R., Ghariani, W., Gharsalli, H., Ghaya Jmii, G., Ghédira, F., Ghédira, A., Ghédira, H., Ghériani, A., Gouta, E. L., Guemira, F., Guermazi, E., Guesmi, A., Hachem, J., Haddad, A., Hakim, K., Hakiri, A., Hamdi, S., Hamed, W., Hamrouni, S., Hamza, M., Haouet, S., Hariz, A., Hendaoui, L., Hfaidh, M., Hriz, H., Hsairi, M., Ichaoui, H., Issaoui, D., Jaafoura, H., Jazi, R., Jazia, R., Jelassi, H., Jerraya, H., Jlassi, H., Jmii, G., Jouini, M., Kâaniche, M., Kacem, M., Kadhraoui, M., Kalai, M., Kallel, K., Kammoun, O., Karoui, M., Karouia, S., Karrou, M., Kchaou, A., Kchaw, R., Kchir, N., Kchir, H., Kechaou, I., Kerrou, M., Khaled, S., Khalfallah, N., Khalfallah, M., Khalfallah, R., Khamassi, K., Kharrat, M., Khelifa, E., Khelil, M., Khelil, A., Khessairi, N., Khezami, M. A., Khouni, H., Kooli, C., Korbsi, B., Koubaa, M. A., Ksantini, R., Ksentini, A., Ksibi, I., Ksibi, J., Kwas, H., Laabidi, A., Labidi, A., Ladhari, N., Lafrem, R., Lahiani, R., Lajmi, M., Lakhal, J., Laribi, M., Lassoued, N., Lassoued, K., Letaif, F., Limaïem, F., Maalej, S., Maamouri, N., Maaoui, R., Maâtallah, H., Maazaoui, S., Maghrebi, H., Mahfoudhi, S., Mahjoubi, Y., Mahjoubi, S., Mahmoud, I., Makhlouf, T., Makni, A., Mamou, S., Mannoubi, S., Maoui, A., Marghli, A., Marrakchi, Z., Marrakchi, J., Marzougui, S., Marzouk, I., Mathlouthi, N., Mbarek, K., Mbarek, M., Meddeb, S., azza mediouni, Mechergui, N., Mejri, I., Menjour, M. B., Messaoudi, Y., Mestiri, T., Methnani, A., Mezghani, I., Meziou, O., Mezlini, A., Mhamdi, S., Mighri, M., Miled, S., Miri, I., Mlayeh, D., Moatemri, Z., Mokaddem, W., Mokni, M., Mouhli, N., Mourali, M. S., Mrabet, A., Mrad, F., Mrouki, M., Msaad, H., Msakni, A., Msolli, S., Mtimet, S., Mzabi, S., Mzoughi, Z., Naffeti, E., Najjar, S., Nakhli, A., Nechi, S., Neffati, E., Neji, H., Nouira, Y., Nouira, R., Omar, S., Ouali, S., Ouannes, Y., Ouarda, F., Ouechtati, W., Ouertani, J., Ouertani, H., Oueslati, A., Oueslati, J., Oueslati, I., Rabai, B., Rahali, H., Rbia, E., Rebai, W., Regaïeg, N., Rejeb, O., Rhaiem, W., Rhimi, H., Riahi, I., Ridha, R., Robbena, L., Rouached, L., Rouis, S., Safer, M., Saffar, K., Sahli, H., Sahraoui, G., Saidane, O., Sakka, D., Salah, H., Sallami, S., Salouage, I., Samet, A., Sammoud, K., Sassi Mahfoudh, A., Sayadi, C., Sayhi, A., Sebri, T., Sedki, Y., Sellami, A., Serghini, M., Sghaier, I., Skouri, W., Slama, I., Slimane, H., Slimani, O., Souhail, O., Souhir, S., Souissi, A., Souissi, R., Taboubi, A., Talbi, G., Tbini, M., Tborbi, A., Tekaya, R., Temessek, H., Thameur, M., Touati, A., Touinsi, H., Tounsi, A., Tounsia, H., Trabelsi, S., Triki, A., Triki, M., Turki, J., Turki, K., Twinsi, H., Walha, Y., Wali, J., Yacoub, H., Yangui, F., Yazidi, M., Youssef, I., Zaier, A., Zainine, R., Zakhama, L., Zalila, H., Zargouni, H., Zehani, A., Zeineb, Z., Zemni, I., Zghal, M., Ziadi, J., Zid, Z., Znagui, I., Zoghlami, C., Zouaoui, C., Zouari, B., Zouiten, L., and Zribi, H.

7. Complex versus simple suicides in Northern Tunisia: A 17 years autopsy-based study.

Author

Kort I, Bchir K, Belleli M, Hmandi O, Mannoubi S, and Allouche M

Subjects
Humans, Tunisia epidemiology, Male, Retrospective Studies, Female, Adult, Sex Distribution, Middle Aged, Age Distribution, Mental Disorders epidemiology, Young Adult, Aged, Accidental Falls mortality, Accidental Falls statistics & numerical data, Suicide statistics & numerical data, Drowning mortality, Suicide, Completed statistics & numerical data, Neck Injuries mortality, Neck Injuries pathology, Asphyxia mortality, Poisoning mortality
Abstract

Background: Complex suicide, whether planned or unplanned, presents significant challenges for forensic specialists and judicial authorities. Although rare, each case requires a thorough examination of both the body and the scene of death. This study aims to analyze the pattern of complex suicide victims in northern Tunisia, comparing them with simple suicide cases., Methods: This was a retrospective observational study with retrospective data collection. We included all cases of simple and complex suicides, autopsied at the Department of Legal Medicine of Tunis, from 2005 to 2021., Results: In total, 72 complex suicides (3.3 % of 2153 suicide fatalities) were identified, with 35 planned and 37 unplanned complex suicides. Males represented 77.8 % of the cases and their mean age was 42.2 ± 15 years. A history of mental illness was reported in 31.9 %. Two suicide methods were used in 93.1 % of cases. The most frequent combination was poisoning and hanging (29.2 %), followed by drowning and falling from a height (12.5 %). The most common single methods were hanging (48.6 %), poisoning (55.6 %), sharp force (31.9 %), and fall from a height (25.0 %). The latter three methods were significantly higher than simple suicides (p < 0.001)., Conclusions: Complex suicide is infrequent and presents significant investigative challenges. A detailed analysis of the victim's background, an on-site inspection of the scene, reconstruction of the events, and a complete autopsy are essential to determine the cause and manner of death., Competing Interests: Conflict of interest statement We have no conflicts of interest to disclose., (Copyright © 2025 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.)

Published
2025
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8. Case Report: Sudden cardiac death due to ventricular myocardial non-compaction.

Author

Mannoubi S, Mesrati MA, Hassen HA, Hasnaoui T, Limem H, Boussaid M, Ben Abdejlil N, and Aissaoui A

Subjects
Adult, Death, Sudden, Cardiac etiology, Echocardiography, Humans, Male, Myocardium, Young Adult, Heart Ventricles diagnostic imaging, Ventricular Dysfunction, Left
Abstract

Ventricular non-compaction (VNC) is a rare myocardium disorder, which can be both genetic and sporadic. A poor wall compaction process or an excessive trabeculae formation may be at the genesis of myocardial hypertrabeculation with multiple recesses. It is often complicated by ventricular dysfunction, arrhythmias and cardiac embolism. Herein we report a case of a 20-year-old male patient with no particular past medical history who was followed up at the cardiology department for dyspnea. Echocardiography showed reduced ejection fraction of the left ventricle with potential hypertrabeculation in the right ventricle, confirmed by cardiac MRI. The patient was not put under medication and was later lost to follow-up. He died few months later without a clear cause explaining death. A forensic autopsy was performed that attributed death to acute ventricle arrhythmia secondary to VNC, emphasizing the major role of an early and specific treatment to avoid such a fatal outcome., Competing Interests: No competing interests were disclosed., (Copyright: © 2020 Mannoubi S et al.)

Published
2020
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9. Abstracts of the 40th National Congress of Medicine Tunis, 19-20 October 2017.

Author

Abdallah M, Abdelaziz A, Abdelaziz O, Abdelhedi N, Abdelkbir A, Abdelkefi M, Abdelmoula L, Abdennacir S, Abdennadher M, Abidi H, Abir Hakiri A, Abou El Makarim S, Abouda M, Achour W, Aichaouia C, Aissa A, Aissa Y, Aissi W, Ajroudi M, Allouche E, Aloui H, Aloui D, Amdouni F, Ammar Y, Ammara Y, Ammari S, Ammous A, Amous A, Amri A, Amri M, Amri R, Annabi H, Antit S, Aouadi S, Arfaoui A, Assadi A, Attia L, Attia M, Attia L, Ayadi I, Ayadi Dahmane I, Ayari A, Azzabi S, Azzouz H, B Mefteh N, B Salah C, Baccar H, Bachali A, Bahlouli M, Bahri G, Baïli H, Bani M, Bani W, Bani MA, Bassalah E, Bawandi R, Bayar M, Bchir N, Bechraoui R, Béji M, Beji R, Bel Haj Yahia D, Belakhel S, Belfkih H, Belgacem O, Belgacem N, Belhadj A, Beltaief N, Beltaief N, Ben Abbes M, Ben Abdelaziz A, Ben Ahmed I, Ben Aissia N, Ben Ali M, Ben Ammar H, Ben Ammou B, Ben Amor A, Ben Amor M, Benatta M, Ben Ayed N, Ben Ayoub W, Ben Charrada N, Ben Cheikh M, Ben Dahmen F, Ben Dhia M, Ben Fadhel S, Ben Farhat L, Ben Fredj Ismail F, Ben Hamida E, Ben Hamida Nouaili E, Ben Hammamia M, Ben Hamouda A, Ben Hassine L, Ben Hassouna A, Ben Hasssen A, Ben Hlima M, Ben Kaab B, Ben Mami N, Ben Mbarka F, Ben Mefteh N, Ben Kahla N, Ben Mrad M, Ben Mustapha N, Ben Nacer M, Ben Neticha K, Ben Othmen E, Ben Rhouma S, Ben Rhouma M, Ben Saadi S, Ben Safta A, Ben Safta Z, Ben Salah C, Ben Salah N, Ben Sassi S, Ben Sassi J, Ben Tekaya S, Ben Temime R, Ben Tkhayat A, Ben Tmim R, Ben Yahmed Y, Ben Youssef S, Ben Ali M, Ben Atta M, Ben Safta Z, Ben Salah M, Berrahal I, Besbes G, Bezdah L, Bezzine A, Bezzine A, Bokal Z, Borsali R, Bouasker I, Boubaker J, Bouchekoua M, Bouden F, Boudiche S, Boukhris I, Bouomrani S, Bouraoui S, Bouraoui S, Bourgou S, Boussabeh E, Bouzaidi K, Chaker K, Chaker L, Chaker A, Chaker F, Chaouech N, Charfi M, Charfi MR, Charfi F, Chatti L, Chebbi F, Chebbi W, Cheikh R, Cheikhrouhou S, Chekir J, Chelbi E, Chelly I, Chelly B, Chemakh M, Chenik S, Cheour M, Cheour M, Cherif E, Cherif Y, Cherif W, Cherni R, Chetoui A, Chihaoui M, Chiraz Aichaouia C, Dabousii S, Daghfous A, Daib A, Daib N, Damak R, Daoud N, Daoud Z, Daoued N, Debbabi H, Demni W, Denguir R, Derbel S, Derbel B, Dghaies S, Dhaouadi S, Dhilel I, Dimassi K, Dougaz A, Dougaz W, Douik H, Douik El Gharbi L, Dziri C, El Aoud S, El Hechmi Z, El Heni A, ELaoud S, Elfeleh E, Ellini S, Ellouz F, Elmoez Ben O, Ennaifer R, Ennaifer S, Essid M, Fadhloun N, Farhat M, Fekih M, Fourati M, Fteriche F, G Hali O, Galai S, Gara S, Garali G, Garbouge W, Garbouj W, Ghali O, Ghali F, Gharbi E, Gharbi R, Ghariani W, Gharsalli H, Ghaya Jmii G, Ghédira F, Ghédira A, Ghédira H, Ghériani A, Gouta EL, Guemira F, Guermazi E, Guesmi A, Hachem J, Haddad A, Hakim K, Hakiri A, Hamdi S, Hamed W, Hamrouni S, Hamza M, Haouet S, Hariz A, Hendaoui L, Hfaidh M, Hriz H, Hsairi M, Ichaoui H, Issaoui D, Jaafoura H, Jazi R, Jazia R, Jelassi H, Jerraya H, Jlassi H, Jmii G, Jouini M, Kâaniche M, Kacem M, Kadhraoui M, Kalai M, Kallel K, Kammoun O, Karoui M, Karouia S, Karrou M, Kchaou A, Kchaw R, Kchir N, Kchir H, Kechaou I, Kerrou M, Khaled S, Khalfallah N, Khalfallah M, Khalfallah R, Khamassi K, Kharrat M, Khelifa E, Khelil M, Khelil A, Khessairi N, Khezami MA, Khouni H, Kooli C, Korbsi B, Koubaa MA, Ksantini R, Ksentini A, Ksibi I, Ksibi J, Kwas H, Laabidi A, Labidi A, Ladhari N, Lafrem R, Lahiani R, Lajmi M, Lakhal J, Laribi M, Lassoued N, Lassoued K, Letaif F, Limaïem F, Maalej S, Maamouri N, Maaoui R, Maâtallah H, Maazaoui S, Maghrebi H, Mahfoudhi S, Mahjoubi Y, Mahjoubi S, Mahmoud I, Makhlouf T, Makni A, Mamou S, Mannoubi S, Maoui A, Marghli A, Marrakchi Z, Marrakchi J, Marzougui S, Marzouk I, Mathlouthi N, Mbarek K, Mbarek M, Meddeb S, Mediouni A, Mechergui N, Mejri I, Menjour MB, Messaoudi Y, Mestiri T, Methnani A, Mezghani I, Meziou O, Mezlini A, Mhamdi S, Mighri M, Miled S, Miri I, Mlayeh D, Moatemri Z, Mokaddem W, Mokni M, Mouhli N, Mourali MS, Mrabet A, Mrad F, Mrouki M, Msaad H, Msakni A, Msolli S, Mtimet S, Mzabi S, Mzoughi Z, Naffeti E, Najjar S, Nakhli A, Nechi S, Neffati E, Neji H, Nouira Y, Nouira R, Omar S, Ouali S, Ouannes Y, Ouarda F, Ouechtati W, Ouertani J, Ouertani J, Ouertani H, Oueslati A, Oueslati J, Oueslati I, Oueslati A, Rabai B, Rahali H, Rbia E, Rebai W, Regaïeg N, Rejeb O, Rhaiem W, Rhimi H, Riahi I, Ridha R, Robbena L, Rouached L, Rouis S, Safer M, Saffar K, Sahli H, Sahraoui G, Saidane O, Sakka D, Salah H, Sallami S, Salouage I, Samet A, Sammoud K, Sassi Mahfoudh A, Sayadi C, Sayhi A, Sebri T, Sedki Y, Sellami A, Serghini M, Sghaier I, Skouri W, Skouri W, Slama I, Slimane H, Slimani O, Souhail O, Souhir S, Souissi A, Souissi R, Taboubi A, Talbi G, Tbini M, Tborbi A, Tekaya R, Temessek H, Thameur M, Touati A, Touinsi H, Tounsi A, Tounsia H, Trabelsi S, Trabelsi S, Triki A, Triki M, Turki J, Turki K, Twinsi H, Walha Y, Wali J, Yacoub H, Yangui F, Yazidi M, Youssef I, Zaier A, Zainine R, Zakhama L, Zalila H, Zargouni H, Zehani A, Zeineb Z, Zemni I, Zghal M, Ziadi J, Zid Z, Znagui I, Zoghlami C, Zouaoui C, Zouari B, Zouiten L, and Zribi H

Published
2017

10. Interplay between two bacterial actin homologs, MamK and MamK-Like, is required for the alignment of magnetosome organelles in Magnetospirillum magneticum AMB-1.

Author

Abreu N, Mannoubi S, Ozyamak E, Pignol D, Ginet N, and Komeili A

Subjects
Adenosine Triphosphatases metabolism, Amino Acid Sequence, Bacterial Proteins genetics, Magnetospirillum cytology, Magnetospirillum genetics, Molecular Sequence Data, Mutation, Actins chemistry, Bacterial Proteins metabolism, Gene Expression Regulation, Bacterial physiology, Magnetosomes physiology, Magnetospirillum metabolism
Abstract

Many bacterial species contain multiple actin-like proteins tasked with the execution of crucial cell biological functions. MamK, an actin-like protein found in magnetotactic bacteria, is important in organizing magnetosome organelles into chains that are used for navigation along geomagnetic fields. MamK and numerous other magnetosome formation factors are encoded by a genetic island termed the magnetosome island. Unlike most magnetotactic bacteria, Magnetospirillum magneticum AMB-1 (AMB-1) contains a second island of magnetosome-related genes that was named the magnetosome islet. A homologous copy of mamK, mamK-like, resides within this islet and encodes a protein capable of filament formation in vitro. Previous work had shown that mamK-like is expressed in vivo, but its function, if any, had remained unknown. Though MamK-like is highly similar to MamK, it contains a mutation that in MamK and other actins blocks ATPase activity in vitro and filament dynamics in vivo. Here, using genetic analysis, we demonstrate that mamK-like has an in vivo role in assisting organelle alignment. In addition, MamK-like forms filaments in vivo in a manner that is dependent on the presence of MamK and the two proteins interact in a yeast two-hybrid assay. Surprisingly, despite the ATPase active-site mutation, MamK-like is capable of ATP hydrolysis in vitro and promotes MamK filament turnover in vivo. Taken together, these experiments suggest that direct interactions between MamK and MamK-like contribute to magnetosome alignment in AMB-1., (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)

Published
2014
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