Search

ABSTRACT: To determine whether differences in positive experiences can influence associative learning ability, 2 tasks were conducted with 90 laying hens at the peak of the laying period. The selected hens were reared in a larger flock under the same housing conditions without perches, so they had the same rearing experiences and were moved to either enriched cages or to the floor system at 16 wk of age. They belonged to 3 breeds (Slovenian barred hen: Ba; brown hen: Br; silver hen, S), with 30 hens per breed. The predictor signal, the sound of a clicker, with a 3-second delayed reinforcer (commercial layer feed) was used to mark the desired behaviour (pecking for feed). Hens that associated feed with a clicker (85.06%) were taught 2 tasks, the colour discrimination task (CD) and the target following task (TF). In the CD, the hens had to discriminate between yellow, red and blue colors and peck at a yellow magnet only. In the TF, the desired behaviour was to follow a target, a purple ball on a stick, from 1 perch to another and peck it at the end of the perch. The main results of the hens associating the signal with feed were that the Ba hens learned faster than the S hens (P = 0.006) and required fewer clicker sounds than the Br hens in the CD (P = 0.003). Floor hens that completed CD or TF or both took less time to complete the task (CD, P = 0.03, TF, P = 0.06; both tasks, P = 0.02) or with fewer clicker sounds (CD task; P = 0.02) than cage hens. Although these results suggest that Ba hens and floor hens showed better associate learning performance, probably because they perceived their environment as more rewarding and thus potentially more positive, caution is needed in interpretation considering social experiences and that the ability to perch and the ability to move quickly on a perch can be confounded in TF and both tasks can be confounded with foraging ability.

Abstract We tested 35 Lipizzan horses older than 5 years, ridden and healthy in three behavioural tests (handling, fear-reaction, and target training test). Physiological (heart rate and heart rate variability) and anatomical measurements (120 head and body distances and angles) were collected to validate parameters that reliably inform on handling/cooperation, fear/exploration and trainability in horses. Utilizing a standard clustering methodology on the behavioural data, we identified four general types of responses and categorised an individual as intermediate, low fearful, horses with low cooperation or low trainability. We additionally analysed the head morphology following Tellington-Jones and Taylor recommendations and correlated the measurements with data from a horse personality questionnaire. Although allocation to a particular personality group was not associated with these two methods, these groups differed in six anatomical characteristics of head and body. Regardless of the group, our results indicated that shorter horses (10.5 cm) are trustworthy, less fearful and easier to handle and train. We also demonstrated that horses with stronger legs and a wider base of the head have a lower heart rate when exposed to the second trial of the handling test.

Inhibitory control describes a multitude of cognitive processes that prevents an impulsive response and enables a more appropriate behavior in a given situation. The ability to inhibit undesirable behaviors, such as aggression, is particularly important in dogs for safe and successful interspecific interaction and cooperation. The present study investigated the associations between two aspects of inhibitory control in dogs, self-control and cognitive inhibition, and the tendency to respond aggressively when provoked. Sixteen police and fourteen privately owned dogs of the same sex, breed group and similar age participated. Self-control, often described as impulsivity, was measured with an exchange paradigm themed the delay of gratification test, and cognitive inhibition with an object discrimination paradigm called the reversal learning test. Aggressive reactivity was assessed with a standardized aggression-eliciting behavior test. When comparing police and privately owned dogs, police dogs showed higher aggression levels and poorer self-control, while the two groups did not differ in cognitive inhibition. Regardless of the dog group, the main results indicated impairments in self-control in dogs with high levels of aggressive reactivity. Dogs showing biting behavior had worse self-control abilities compared to dogs with no signs of aggression. No association between cognitive inhibition and aggression was found. We conclude that self-control, measured as the ability to tolerate delayed rewards, appears to be an important aspect of inhibitory control involved in the tendency to respond aggressively, particularly in police dogs.

Damaging behaviors (DB) such as tail and ear biting are prevalent in pig production and reduce welfare and performance. Anecdotal reports suggest that health challenges increase the risk of tail-biting. The prevalence of tail damage and health problems show high correlations across batches within and between farms. There are many common risk factors for tail-biting and health problems, notably respiratory, enteric and locomotory diseases. These include suboptimal thermal climate, hygiene, stocking density and feed quality. The prevalence of tail damage and health problems also show high correlations across batches within and between farms. However, limited evidence supports two likely causal mechanisms for a direct link between DB and health problems. The first is that generalized poor health (e.g., enzootic pneumonia) on farm poses an increased risk of pigs performing DB. Recent studies indicate a possible causal link between an experimental inflammation and an increase in DB, and suggest a link between cytokines and tail-biting. The negative effects of poor health on the ingestion and processing of nutrients means that immune-stimulated pigs may develop specific nutrient deficiencies, increasing DB. The second causal mechanism involves tail-biting causing poor health. Indirectly, pathogens enter the body via the tail lesion and once infected, systemic spread of infection may occur. This occurs mainly via the venous route targeting the lungs, and to a lesser extent via cerebrospinal fluid and the lymphatic system. In carcasses with tail lesions, there is an increase in lung lesions, abscessation, arthritis and osteomyelitis. There is also evidence for the direct spread of pathogens between biters and victims. In summary, the literature supports the association between poor health and DB, particularly tail-biting. However, there is insufficient evidence to confirm causality in either direction. Nevertheless, the limited evidence is compelling enough to suggest that improvements to management and housing to enhance pig health will reduce DB. In the same way, improvements to housing and management designed to address DB, are likely to result in benefits to pig health. While most of the available literature relates to tail-biting, we suggest that similar mechanisms are responsible for links between health and other DB.

Observations of play in animals have been suggested as a promising indicator of positive emotions and thus of positive animal welfare. However, if play can follow the proposed reward cycle concept where animals estimate and value reward differently in different phases of the cycle (anticipation, consummation and post-consummation) is unclear. To investigate if a reward cycle for play exists in growing pigs, we carried out an exploratory study where pigs were tested when they were naïve to a reward cycle test (first occasion) against when they were accustomed to going through the test after having the access to an open play arena with objects. Forty undocked pigs were housed in a weaner stable with two castrated males and two females per pen. Within each litter, we randomly selected and tested one male and one female test pig, each being tested as naïve or accustomed to the testing environment. The first week the pigs (n = 20) were tested four times and regarded as naïve during the first day. After that they were regarded as accustomed, and were tested twice a week for 3 weeks. We observed the behavior of the tested pairs in three subsequent stages: (1) in a holding pen 3 min, (2) in a play arena 15 min, and (3) in their home pen 10 min. When accustomed, pigs showed more locomotor play, social interactions and standing, and a tendency of more orientation toward the play arena and exploring bars facing the play arena (i.e., reward-seeking behavior) in the holding pen than when they were naïve, suggesting an anticipation to enter the play arena. Performing high numbers of object play in all sessions, and for accustomed pigs more exploration and social interaction, but less locomotor play and walking in the play arena may suggest consumption of play and exploration. Finding more lying and sitting in accustomed pigs, but less standing and walking in the home pen is in line with the previous hypothesis of the post-consummatory behaviors. Our study showed mixed results for the existence of a reward cycle for play in pigs and generated questions for future research.

Our aim was to investigate cardiovascular activation by measuring changes in facial and body surface temperature using infrared thermography, and neuroendocrine activation using salivary cortisol (CORT) and serotonin concentration (SER) in dogs exhibiting aggressive reactivity in real time. Based on two factors, owner-reported past aggressive behaviors, and detailed behavioral observations collected during a Socially Acceptable Behavior test consisting of 16 subtests and, each individual was categorized as aggressive or non-aggressive. CORT and SER showed no difference in neuroendocrine activity between dogs, but aggressive dogs with higher levels of aggression were found to have lower SER. Aggressive dogs also had an increase in facial temperature from pre-test values. The discovery of a correlation between tail wagging and left tail wagging with aggression level and aggression-related behaviors in aggressive dogs is further evidence of the right hemisphere specialization for aggression previously reported in the literature. This study provides the first evidence that both cardiovascular and neuroendocrine systems are activated during an active act of aggression in dogs.

Abnormalities in bone development in humans and non-humans can lead to impaired physical and psychological health; however, evidence is lacking regarding the role of individual psychosocial factors in the development of poor bone conditions. Addressing this lack of knowledge, we used low-productive laying hens (n = 93) and assessed behavioral responses to an open-field test [at 17, 18, 29, 33 weeks of age (wa)], an aerial predator test (at 39 wa), and a social reinstatement test (at 42 wa). Bone condition was assessed using a palpation technique on five occasions (at 16, 29, 33, 45, 58 wa), with half of the hens experiencing damage (deviations, fractures, or both) at 29 wa and all hens by 58 wa. Corticosterone (CORT) concentration in feathers (at 16, 33, 58 wa) and body weight (at 23, 47, 58 wa) were also investigated. We hypothesized that lighter birds (at 23 wa) with higher CORT (at 16 wa) and open field-induced fear collected before the onset of lay (at 17 and 18 wa) are associated with a worse bone condition when in lay. We also hypothesized that those birds with more damage at the peak of laying (at 29 wa) would be lighter at 47 and 58 wa and more fearful by showing higher open field-induced (at 29 and 33 wa) and predator-induced fear responses, however, acting less socially toward conspecifics. These hens were also expected to have higher CORT (at 33 and 58 wa). Our results show no association between open-field fear level and fear behavior, CORT concentration, or body weight on the one hand (all measured before starting to lay) and bone damage at 29 wa on the other. When in lay, bone damage was associated with more pecking and less crossing zones when faced with an open-field situation at 29 wa and improved sociality at 42 wa. This study provides the first evidence of a relationship of bone health with fear, sociality, and stress response. When in poor bone condition, our hens had enhanced psychological stress measured by fear behavior reactivity but not physiological stress measured as feather CORT concentration.

Feather pecking is a behavior that occurs in order to cope with a constrained environment and is a serious problem in the egg production industry. This longitudinal study was conducted under commercial conditions to investigate whether the application of two repellent mixtures, previously suggested as aversive to wild birds, to the plumage of Prelux-R hybrid egg layers is a viable alternative to beak trimming as a solution to discourage feather pecking among laying hens. A total of 180 untrimmed hybrid layers was reared together in a floor pen. At 18 weeks of age they were allocated randomly to three treatments (repellent P, repellent T, control), each consisting of 6 replicated enriched cages with 10 hens in each cage. Hens were evenly sprayed once every 2 weeks for 54 weeks with solution P (dimethyl anthranilate and methyl phenylacetate), solution T (dimethyl anthranilate and geraniol), or distilled water (control). Body weight, plumage condition, behavior, feed intake, and egg quality measurements were taken at five time periods from 26 to 76 weeks of age. Egg production and mortality were recorded daily. The treatments did not affect feather pecking behavior. Hens treated with repellent T tended to perform less cage pecking than the control hens. The use of the repellents did not reduce feather pecking, the plumage was even more significantly damaged in the hens given the repellents compared to the control hens. This suggests the chemicals in the repellents worsened the plumage. No differences in feed intake and daily egg production between treatments were found. Raw and hard-boiled eggs were highly uniform in odor/flavor/taste and no offensive odor absorption related to the chemicals in the repellents was detected. In conclusion, in the present study we did not find any beneficial effect of dimethyl anthranilate-based repellents on feather pecking frequency and plumage/feather condition. Therefore, we do not encourage their use in wider commercial settings.

The study was conducted between March and September 2019 in six meat-type turkey flocks with similar management standard procedures using the transect walk method. The concept of the method is based on visual observation of the birds while slowly walking across the entire farm in predetermined transects. Each flock was evaluated at three different times during the fattening cycle: at 3 to 4, 12 to 13, and 19 to 20 weeks of age, and total number of males and females that were immobile or lame, had visible head, vent, or back wounds, were small, featherless, dirty, or sick, had pendulous crop, or showed aggression toward birds or humans were recorded. At each visit, NH3 and CO2 were measured within the facilities. In the first assessment, the most frequently observed welfare indicators were small size (0.87%) and immobility (0.08%). Males showed a significantly higher prevalence of small size (p < 0.01), sickness (p < 0.05), and dirtiness (p < 0.1) compared to females. In the second assessment, the most common findings in both sexes were dirtiness (1.65%) and poor feather condition (1.06%), followed by immobility (0.28%). Males were significantly dirtier (p < 0.001), had more immobile birds (p < 0.01) and birds with vent wounds (p < 0.1), but had fewer sick birds (p < 0.05). In the last assessment, an increase in immobile, lame, sick, and dead birds was recorded, indicating an increase in health problems. Higher CO2 (3000 and 4433 ppm) and NH3 (40 and 27.6 ppm) values were noted only at the first assessment in two facilities. Further analyses showed that slightly elevated NH3 and CO2 levels did not influence the occurrence of welfare indicators. This study is the first description of the welfare of commercial turkey flocks in Slovenia.