24 results on '"Mandrile L"'
Search Results
2. Albumin and fibronectin adsorption on treated titanium surfaces for osseointegration: An advanced investigation
- Author
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Barberi, J., primary, Mandrile, L., additional, Napione, L., additional, Giovannozzi, A.M., additional, Rossi, A.M., additional, Vitale, A., additional, Yamaguchi, S., additional, and Spriano, S., additional
- Published
- 2022
- Full Text
- View/download PDF
3. Vibrational spectroscopy
- Author
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Mino, L., Mandrile, L., Iannarelli, L., Portesi, C., Martra, G., and Rossi, A. M.
- Subjects
Raman microscopy ,IR spectroscopy ,Lewis acidity ,Probe molecule ,Surface hydration ,TiO2 ,nanoparticles - Published
- 2020
4. Near-infrared spectroscopy as a new method for post-harvest monitoring of white truffles
- Author
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Mandrile L., Mello A., Vizzini A., Balestrini R., and Rossi A.M.
- Subjects
Tuber magnatum ,Near-infrared spectroscopy ,Maturation ,NIR imaging ,Chemometrics ,Tuber bochii - Abstract
The quality of truffles is related to their maturation stage, and good maturation degree is important in order to have a truffle with valuable organoleptic properties. An innovative, rapid and reliable technique to determine the maturation degree of truffles and monitor their post-harvest ageing process is proposed. Near-infrared (NIR) spectroscopy was used for the first time to monitor the post-harvest ripening of white truffles of two different species (Tuber magnatum and T. borchii). Optical microscopy was flanked by NIR spectroscopy imaging to monitor fruiting bodies at different degrees of maturation. The NIR profile of truffles provided statistical differentiation between the biochemical composition of different tissues (vegetative hyphae and ascospores). The optical evaluation of the degree of maturation was correlated to spectral information in order to obtain a non-operator dependent, rapid, simple and cost-effective method to evaluate the degree of maturation of white truffles. Partial least square regression (PLS) was used for multivariate calibration of NIR spectra against maturation degree, and ripening curves were obtained (with correlation coefficients R2 = 0.95 for T. magnatum and R2 = 0.76 for T. borchii). Moreover, a classification method based on PLS discriminant analysis of truffle in three maturity stages was developed, achieving a total of correct classification rate of 83%.
- Published
- 2020
5. Chemometric handling of Raman spectra for live systems monitoring and susceptibility tests
- Author
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Mandrile L., Noris E., Barzan G., Sacco A., Giovannozzi A., Rotunno S., Miozzi L., Vaira A.M., and Rossi A.
- Subjects
no key words - Abstract
no abstract
- Published
- 2019
6. Combining Raman Spetroscopy and Dielectrophoresis for rapid determination of bacterial antibiotic susceptibility
- Author
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Barzan, G., Sacco, A., Mandrile, L., Giovannozzi, A. M., and Rossi, C. Portesi and A. M.
- Published
- 2019
7. Combining Raman Spectroscopy and Dielectrophoresis for rapid determination of bacterial susceptibility to antibiotics
- Author
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Barzan, G., Sacco, A., Mandrile, L., Giovannozzi, A. M., Brown, J., Portesi, C., Hardie, K., Alexander, M., Williams, P., and Andrea Mario Rossi
- Published
- 2019
8. Non-destructive Raman spectroscopy for early monitoring of virus infection in plants
- Author
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Mandrile L. Rotunno S., Miozzi L., Rossi A.M., and Noris E.
- Subjects
fungi ,food and beverages ,no key words - Abstract
Plant viruses are obligate biotrophic pathogens infecting a wide variety of plant species. To support their propagation and proliferation, they reprogram the plant cell metabolism, causing morphological and physiological alterations in the infected hosts, eventually leading to reduced crop yield and poor product quality. Among the wide variety of symptoms induced by plant viruses, the most common is leaf chlorosis, associated to depleted photosynthetic activity. Early virus detection can contribute to limit virus spread and economic impact. However, currently used diagnostic procedures, such as immunological or nucleic acid-based techniques are time-consuming, destructive and expensive. Raman spectroscopy (RS) is a non-destructive procedure that generates a chemical fingerprinting of a sample, at low operating costs. Here, we used RS combined to chemometric analysis to evaluate the metabolic changes occurring during the infection of an economically relevant crop, tomato, by two dangerous viral pathogens, Tomato yellow leaf curl Sardinia virus (TYLCSV) and Tomato spotted wilt virus (TSWV). Coherent modifications of the entire spectral profile were detected in regions associated with chlorophylls as well as carotenoids, carbohydrates, and phenolic compounds, allowing to discriminate healthy plants from specimens infected by both viruses, in a time frame of 4 weeks after virus inoculum. RS could not only detect metabolic changes occurring at early and still asymptomatic stages of infection (with >70% accuracy at 14 days after inoculation for TYLCSV and >85% at 8 days for TSWV), but allowed also to differentiate spectral variations induced by the two viruses. This investigation provides interesting clues to test the efficacy of RS to monitor the response of plants to biotic or abiotic stimuli, with particular attention to the photosynthetic process.
- Published
- 2019
9. Direct detection and quantification of molecular surface contaminants by infrared and Raman spectroscopy
- Author
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Mandrile, L., primary, Giovannozzi, A. M., additional, Pennecchi, F., additional, Saverino, A., additional, Lobascio, C., additional, and Rossi, A. M., additional
- Published
- 2015
- Full Text
- View/download PDF
10. Rapid and sensitive detection of pyrimethanil residues on pome fruits by Surface Enhanced Raman Scattering.
- Author
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Giovannozzi, A.M., Durbiano, F., Rossi, A.M., Mandrile, L., and Martra, G.
- Subjects
- *
APPLES , *PYRIMETHANIL , *SERS spectroscopy , *RAMAN spectroscopy technique , *GOLD nanoparticles , *PARTIAL least squares regression - Abstract
Surface Enhanced Raman Scattering (SERS) supported by gold nanoparticles (AuNPs) was applied to detect and quantify residues of pyrimethanil on pome fruits, a widely used fungicide in horticultural species. Spheroidal AuNPs with different size were fabricated and compared in this study. The analytical procedure was set up on a silicon dioxide flat substrate to standardize SERS methodology. A Raman mapping strategy was exploited to increase signal reproducibility and to minimize bias due to different local surface morphologies. Univariate and multivariate regressions were compared for calibration. Multivariate PLS approach demonstrated acceptable repeatability and method stability (RMSECV = 4.79 ppm; RMSEP = 4.31 ppm) in the range 0–40 mg kg −1 , providing higher accuracy and intra-day repeatability with a mean percentage error of 18.7% and 32.8% for PLS and univariate calibration, respectively. The method here proposed can be reliably applied for PMT detection on pome fruits within the European law limits. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
11. Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses.
- Author
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Mandrile L, D'Errico C, Nuzzo F, Barzan G, Matić S, Giovannozzi AM, Rossi AM, Gambino G, and Noris E
- Abstract
Grapevine is one of the most cultivated fruit plant among economically relevant species in the world. It is vegetatively propagated and can be attacked by more than 80 viruses with possible detrimental effects on crop yield and wine quality. Preventive measures relying on extensive and robust diagnosis are fundamental to guarantee the use of virus-free grapevine plants and to manage its diseases. New phenotyping techniques for non-invasive identification of biochemical changes occurring during virus infection can be used for rapid diagnostic purposes. Here, we have investigated the potential of Raman spectroscopy (RS) to identify the presence of two different viruses, grapevine fan leaf virus (GFLV) and grapevine rupestris stem pitting-associated virus (GRSPaV) in Vitis vinifera cv. Chardonnay. We showed that RS can discriminate healthy plants from those infected by each of the two viruses, even in the absence of visible symptoms, with accuracy up to 100% and 80% for GFLV and GRSPaV, respectively. Chemometric analyses of the Raman spectra followed by chemical measurements showed that RS could probe a decrease in the carotenoid content in infected leaves, more profoundly altered by GFLV infection. Transcriptional analysis of genes involved in the carotenoid pathway confirmed that this biosynthetic process is altered during infection. These results indicate that RS is a cutting-edge alternative for a real-time dynamic monitoring of pathogens in grapevine plants and can be useful for studying the metabolic changes ensuing from plant stresses., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Mandrile, D’Errico, Nuzzo, Barzan, Matić, Giovannozzi, Rossi, Gambino and Noris.)
- Published
- 2022
- Full Text
- View/download PDF
12. Efficacy, biocompatibility and degradability of carbon nanoparticles for photothermal therapy of lung cancer.
- Author
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Kokalari I, Keshavan S, Rahman M, Gazzano E, Barzan G, Mandrile L, Giovannozzi A, Ponti J, Antonello G, Monopoli M, Perrone G, Bergamaschi E, Riganti C, Fadeel B, and Fenoglio I
- Subjects
- A549 Cells, Carbon, Cell Line, Tumor, Humans, Photothermal Therapy, Lung Neoplasms therapy, Nanoparticles
- Abstract
Aim: To investigate near infrared-induced phototoxicity toward lung cancer cells, and the biodegradability and effect on immune cells of glucose-derived carbon nanoparticles (CNPs). Methods: The human A549 lung adenocarcinoma cell line was used as a model to study the phototoxicity of CNPs. The biodegradability and the effect on immune cells was demonstrated in primary human neutrophils and macrophages. Results: Near infrared-activated CNPs elicited rapid cell death, characterized by the elevation of heat shock proteins and the induction of DNA damage. CNPs were found to be noncytotoxic toward primary human macrophages and were susceptible to biodegradation when cocultured with human neutrophils. Conclusions: Our results identify CNPs as promising platforms for photothermal therapy of lung cancer.
- Published
- 2021
- Full Text
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13. Clinical characteristics and evolution of hematological patients and COVID-19 in Argentina: a report from the Argentine Society of Hematology.
- Author
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Basquiera AL, García MJ, Martinez Rolón J, Olmedo J, Laviano J, Burgos R, Caeiro G, Remaggi G, Raña P, Paoletti M, González CM, Fernández I, Pavlovsky A, Perusini MA, Rodriguez A, Guanchiale L, Carvani A, Mandrile L, Figueroa F, Vicente Reparaz A, Fragapane Mathus PN, Garate G, Fauque ME, Kantor G, Cruset S, Gonzalez Lorch JS, Szelagowski M, Giarini MP, Oliveira N, García MC, Ventriglia MV, Pereyra PH, Gutierrez DR, Kusminsky G, Troccoli J, Freitas MJ, Cranco S, Del V Sanchez N, Rey I, Funes ME, Jarchum S, Freue J, Miroli A, Guerrero O, López Ares L, Campestri R, Bove V, Salinas GN, Cabrejo M, Milone JH, Zabaljauregui S, Gotta D, Dupont JC, and Stemmelin G
- Subjects
- Argentina epidemiology, COVID-19 Testing, Humans, Middle Aged, SARS-CoV-2, COVID-19, Hematology
- Abstract
Individuals with malignancies and COVID-19 have a lower survival compared with the general population. However, the information about the impact of COVID-19 on the whole hematological population is scarce. We aimed to describe the 30th day overall survival (OS) after COVID-19 infection in patients with a hematological disease in Argentina. A completely anonymous survey from the Argentine Society of Hematology was delivered to all the hematologists in Argentina; it started in April 2020. A cut-off to analyze the data was performed in December 2020 and, finally, 419 patients were reported and suitable for the analysis (average age: 58 years, 90% with malignant diseases). After the COVID-19 diagnosis, the 30-day OS for the whole population was 80.2%. From the entire group (419), 101 (24.1%) individuals required intensive care unit admission, where the 30-day OS was 46.6%. Among allogeneic stem cell transplant recipients, the 30-day OS was 70.3%. Factors associated with a low OS were two or more comorbidities, an active hematological disease and history of chemotherapy. In individuals with the three factors, the 30-day OS was 49.6% while the 30-day OS in those without those factors was 100%. Patients with hematological diseases have a higher mortality than the general population. This group represents a challenge and requires careful decision-making of the treatment in order not to compromise the chances of cure.
- Published
- 2021
14. Migration study of organotin compounds from food packaging by surface-enhanced Raman scattering.
- Author
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Mandrile L, Vona M, Giovannozzi AM, Salafranca J, Martra G, and Rossi AM
- Subjects
- Food Packaging, Silver, Spectrum Analysis, Raman, Metal Nanoparticles, Organotin Compounds
- Abstract
The potential of surface-enhanced Raman scattering (SERS) has been investigated for the rapid analysis of two representative organotin compounds (OTCs): dibutyltin maleate (DTM) and tributyltin chloride (TBT), after migration tests from polyvinyl chloride (PVC), as a model food packaging material in aqueous food simulant (acetic acid 3% w/v). OTCs, often used as heat stabilizers additives for PVC, are classified as endocrine disrupting chemicals (EDCs) and their migration potential has to be controlled in compliance with the normative prescriptions for food contact materials. In this study, colloidal silver nanoparticles (AgNPs) were applied as liquid SERS substrate for direct-in-liquid analysis of food simulant after standardized migration tests of PVC samples spiked with OTCs. Promising results were obtained, reaching detection limits below the permitted limits for the considered OTCs (i.e. 0.15 mg/l): DTM and TBT were detected down to 0.01 mg/l and 0.08 mg/l, respectively. Calibration curves were calculated for standard solutions of DTM and TBT in the dynamic range between 0 and 1 mg/l (reduced χ
2 = 0.8), and 0.5-5 mg/l (reduced χ2 = 0.2), respectively. Migrated TBT and DTM were detected in the food simulant, specifically identified and quantified by SERS, with a measurement uncertainty around 10% in all cases. In particular, it was found that TBT can migrate in higher amount compared to DTM when the PVC film is in contact with a slightly acidic matrix. These results were further confirmed by inductively coupled plasma-mass spectrometry and UV-Vis spectroscopy. In the present study, direct-in-liquid SERS approach showed to be very promising because it provides a fast response and it allows to overcome most of the common drawbacks of solid SERS substrates due to inhomogeneity problems and low repeatability., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
15. Direct quantification of sulfur dioxide in wine by Surface Enhanced Raman Spectroscopy.
- Author
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Mandrile L, Cagnasso I, Berta L, Giovannozzi AM, Petrozziello M, Pellegrino F, Asproudi A, Durbiano F, and Rossi AM
- Subjects
- Colloids chemistry, Limit of Detection, Metal Nanoparticles chemistry, Silver chemistry, Solid Phase Extraction, Spectrum Analysis, Raman, Sulfur Dioxide analysis, Wine analysis
- Abstract
A rapid Surface Enhanced Raman Spectroscopy (SERS) method to detect SO
2 in wine is presented, exploiting the preferential binding of silver nanoparticles (AgNPs) with sulfur-containing species. This interaction promotes the agglomeration of the AgNPs and inducing the formation of SERS "hot spots" responsible for SO2 signals enhancement. For increasing SO2 concentrations from 0 to100 mg/l in wine simulant, SERS intensity showed an increasing trend, following a Langmuir absorption function (R2 = 0.94). Due to the wine matrix variability, a standard additions method was then employed for quantitative analysis in red and white wines. This method does not require the SO2 separation but only a matrix pre-cleaning by solid phase extraction. The limit of detection (LOD) was defined for each wine tested, ranging from 0.6 mg/l to 9.6 mg/l. The results obtained were validated by comparison with the International Organization of Vine and Wine method (OIV-MA-AS323-04A)., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
16. In situ seed-growth synthesis of silver nanoplates on glass for the detection of food contaminants by surface enhanced Raman scattering.
- Author
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D'Agostino A, Giovannozzi AM, Mandrile L, Sacco A, Rossi AM, and Taglietti A
- Subjects
- Calibration, Glass chemistry, Malus chemistry, Silver chemistry, Spectrum Analysis, Raman, Surface Properties, Food Contamination analysis, Fungicides, Industrial analysis, Metal Nanoparticles chemistry, Seeds growth & development, Silver metabolism, Thiram analysis
- Abstract
Seed-growth synthesis is a common strategy to prepare silver nanoplates, whose peculiar plasmonic features can be exploited for surface enhanced Raman scattering (SERS) applications. Here we describe the fabrication and characterization of SERS chips using a peculiar in situ seed growth method, yielding a dense layer of nano-objects directly on a glass slide. In this way, geometric features (i.e. shape and dimensions) of the nano-objects can be tuned by controlling the growth time, obtaining a high concentration of hot spots on the surface. In particular, the SERS response of four kinds of chips were investigated to define the best SERS configuration in terms of size of the silver nano-objects, excitation wavelength and homogeneity of the SERS response. Silver nano-plates with a seeded growth time of 60 min demonstrated remarkable results both in terms of plasmonic enhancement, with an enhancement factor (EF) of 2 × 10
5 using a 532 nm laser excitation, and good homogeneity of the SERS response with intra- and inter-maps RSD of 10% and 5%, respectively. In order to demonstrate application of these chips for real sample analysis, an analytical procedure for the detection of a model pesticide, i.e. thiram fungicide, was developed and applied to its detection on green apples peels. SERS measurements on 60 min seeded growth silver nano-plates chip coupled with a multivariate PLS approach demonstrated high accuracy and repeatability for thiram detection in food matrix within the European law limits., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
17. Flexible and Transparent Substrates Based on Gold Nanoparticles and TiO 2 for in Situ Bioanalysis by Surface-Enhanced Raman Spectroscopy.
- Author
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Mandrile L, Giovannozzi AM, Sacco A, Martra G, and Rossi AM
- Subjects
- Humans, Spectrum Analysis, Raman, Surface Properties, Biosensing Techniques, Gold chemistry, Metal Nanoparticles chemistry, Pesticides analysis, Pyrimidines analysis, Titanium chemistry
- Abstract
Flexible and transparent substrates are emerging as low cost and easy-to-operate support for surface-enhanced Raman spectroscopy (SERS). In particular, in situ SERS detection approach for surface characterization in transmission modality can be efficiently employed for non-invasive analysis of non-planar surfaces. Here we propose a new methodology to fabricate a homogenous, transparent, and flexible SERS membrane by the assistance of a thin TiO
2 porous layer deposited on the PDMS surface, which supports the uniform loading of gold nanoparticles over large area. The substrate was first characterized for homogeneity, sensitivity and repeatability using a model molecule for SERS, i.e., 7-mercapto-4-methylcoumarin. Satisfactory intra-substrate uniformity and inter-substrates repeatability was achieved, showing an RSD of 10%, and an analytical sensitivity down to 10 nM was determined with an EF of 3.4 × 105 ± 0.4 × 105 . Furthermore, SERS detection of pyrimethanil (PMT), a commonly employed pesticide in crops for human consumption, was performed in situ, exploiting the optical transparency of the device, using both model surfaces and non-flat bio-samples. PMT contamination at the phytochemical concentration levels corresponding to commonly used infield doses was successfully detected on the surface of the yellow Ficus benjiamina leaves, supporting the use of this substrate for food safety in-field application.- Published
- 2019
- Full Text
- View/download PDF
18. Authentication of cocoa bean shells by near- and mid-infrared spectroscopy and inductively coupled plasma-optical emission spectroscopy.
- Author
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Mandrile L, Barbosa-Pereira L, Sorensen KM, Giovannozzi AM, Zeppa G, Engelsen SB, and Rossi AM
- Subjects
- Africa, Central, Discriminant Analysis, Ecuador, Least-Squares Analysis, Principal Component Analysis, Spectroscopy, Fourier Transform Infrared statistics & numerical data, Cacao chemistry, Food Analysis methods, Spectroscopy, Fourier Transform Infrared methods, Spectroscopy, Near-Infrared methods
- Abstract
The aim of this study was to evaluate the efficacy of a multi-analytical approach for origin authentication of cocoa bean shells (CBS). The overall chemical profiles of CBS from different origins were characterized using diffuse reflectance near-infrared spectroscopy (NIRS) and attenuated total reflectance mid-infrared spectroscopy (ATR-FT-IR) for molecular composition identification, as well as inductively coupled plasma-optical emission spectroscopy (ICP-OES) for elemental composition identification. Exploratory chemometric techniques based on Principal Component Analysis (PCA) were applied to each single technique for the identification of systematic patterns related to the geographical origin of samples. A combination of the three techniques proved to be the most promising approach to establish classification models. Partial Least Squares-Discriminant Analysis modelling of fused PCA scores of three independent models was used and compared with single technique models. Improved classification of CBS samples was obtained using the fused model. Satisfactory classification rates were obtained for Central African samples with an accuracy of 0.84., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
19. Nondestructive Raman Spectroscopy as a Tool for Early Detection and Discrimination of the Infection of Tomato Plants by Two Economically Important Viruses.
- Author
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Mandrile L, Rotunno S, Miozzi L, Vaira AM, Giovannozzi AM, Rossi AM, and Noris E
- Subjects
- Begomovirus metabolism, Solanum lycopersicum virology, Spectrum Analysis, Raman methods, Tospovirus metabolism, Solanum lycopersicum metabolism, Plant Diseases virology
- Abstract
Global population forecasts dictate a rapid adoption of multifaceted approaches to fulfill increasing food requirements, ameliorate food dietary value and security using sustainable and economically feasible agricultural processes. Plant pathogens induce up to 25% losses in vegetable crops and their early detection would contribute to limit their spread and economic impact. As an alternative to time-consuming, destructive, and expensive diagnostic procedures, such as immunological assays and nucleic acid-based techniques, Raman spectroscopy (RS) is a nondestructive rapid technique that generates a chemical fingerprinting of a sample, at low operating costs. Here, we assessed the suitability of RS combined to chemometric analysis to monitor the infection of an important vegetable crop plant, tomato, by two dangerous and peculiarly different viral pathogens, Tomato yellow leaf curl Sardinia virus (TYLCSV) and Tomato spotted wilt virus (TSWV). Experimentally inoculated plants were monitored over 28 days for symptom occurrence and subjected to RS analysis, alongside with measuring the virus amount by quantitative real-time PCR. RS allowed to discriminate mock inoculated (healthy) from virus-infected specimens, reaching an accuracy of >70% after only 14 days after inoculation for TYLCSV and >85% only after 8 days for TSWV, demonstrating its suitability for early detection of virus infection. Importantly, RS also highlighted spectral differences induced by the two viruses, providing specific information on the infecting agent.
- Published
- 2019
- Full Text
- View/download PDF
20. Comprehensive study on the degradation of ochratoxin A in water by spectroscopic techniques and DFT calculations.
- Author
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Cagnasso I, Tonachini G, Berto S, Giacomino A, Mandrile L, Maranzana A, and Durbiano F
- Abstract
Ochratoxin A (OTA) is one of the most important dietary risk factors and is classified as a possible carcinogen to humans. Assessing the conditions to remove it from foodstuffs in a simple and effective way is of the utmost importance. OTA behaviour in water in the pH range 1.0-12.5 was elucidated to investigate the conditions for irreversible toxicity inactivation of OTA. The results indicate that four forms, from neutral to trianionic, intervene depending on the pH. p K
a1,2 were rigorously established by independent spectroscopic techniques to overcome the scarcity of literature. Then, Density Functional Theory (DFT) calculations were used to determine the most probable degradation mechanism and this was confirmed by fluorescence spectroscopy. At pH 12.5, hydrolyzation of the lactone ring starts in less than one hour, but only after two hours does the degradation process lead to fragmentation. After one week this process is not yet completed. The reaction products occurring upon re-acidification were also investigated. OTA degradation is still reversible if acidic conditions are promptly restored, yielding again a hazardous molecule. However, degradation becomes irreversible after fragmentation. This finding suggests proceeding with due caution if a base is exploited to remove the toxin., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)- Published
- 2019
- Full Text
- View/download PDF
21. Detection of insect's meal in compound feed by Near Infrared spectral imaging.
- Author
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Mandrile L, Fusaro I, Amato G, Marchis D, Martra G, and Rossi AM
- Subjects
- Animals, Automation, Discriminant Analysis, Fourier Analysis, Insecta, Animal Feed analysis, Spectroscopy, Near-Infrared
- Abstract
Insects have recently emerged as a new protein source for both food and feed. Some studies have already demonstrated that insects' meal can be successfully added to animal feed without threaten animals' growth indices. However, effective and validated tests to individuate insects' meal in feed are strongly needed to meet traceability and safety concerns and to support the European legislation under development. Spectroscopic techniques represent valuable rapid and non-destructive methods that can be applied for in-situ analysis in feed production plants or in farms. In this work a Fourier Transform Near Infrared spectroscopy imaging (FT NIR) as a potential screening method for the detection and quantification of insects' meal in feed is presented. Discriminant analysis was used for the automatic recognition of insects' meal fragments into the feed matrix. Moreover, the possibility to quantify insect's meal in feed sample was successfully tested. The proposed method is a rapid and green strategy for feed contamination screening analysis., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
22. Influence of the long-range ordering of gold-coated Si nanowires on SERS.
- Author
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Cara E, Mandrile L, Ferrarese Lupi F, Giovannozzi AM, Dialameh M, Portesi C, Sparnacci K, De Leo N, Rossi AM, and Boarino L
- Abstract
Controlling the location and the distribution of hot spots is a crucial aspect in the fabrication of surface-enhanced Raman spectroscopy (SERS) substrates for bio-analytical applications. The choice of a suitable method to tailor the dimensions and the position of plasmonic nanostructures becomes fundamental to provide SERS substrates with significant signal enhancement, homogeneity and reproducibility. In the present work, we studied the influence of the long-range ordering of different flexible gold-coated Si nanowires arrays on the SERS activity. The substrates are made by nanosphere lithography and metal-assisted chemical etching. The degree of order is quantitatively evaluated through the correlation length (ξ) as a function of the nanosphere spin-coating speed. Our findings showed a linear increase of the SERS signal for increasing values of ξ, coherently with a more ordered and dense distribution of hot spots on the surface. The substrate with the largest ξ of 1100 nm showed an enhancement factor of 2.6 · 10
3 and remarkable homogeneity over square-millimetres area. The variability of the signal across the substrate was also investigated by means of a 2D chemical imaging approach and a standard methodology for its practical calculation is proposed for a coherent comparison among the data reported in literature.- Published
- 2018
- Full Text
- View/download PDF
23. Species-specific detection of processed animal proteins in feed by Raman spectroscopy.
- Author
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Mandrile L, Amato G, Marchis D, Martra G, and Rossi AM
- Subjects
- Animals, Cattle, Species Specificity, Animal Feed analysis, Food Contamination analysis, Spectrum Analysis, Raman methods
- Abstract
The existing European Regulation (EC n° 51/2013) prohibits the use of animals meals in feedstuffs in order to prevent Bovine Spongiform Encephalopathy infection and diffusion, however the legislation is rapidly moving towards a partial lifting of the "feed ban" and the competent control organisms are urged to develop suitable analytical methods able to avoid food safety incidents related to animal origin products. The limitations of the official methods (i.e. light microscopy and Polymerase Chain Reaction) suggest exploring new analytic ways to get reliable results in a short time. The combination of spectroscopic techniques with optical microscopy allows the development of an individual particle method able to meet both selectivity and sensitivity requirements (0.1%w/w). A spectroscopic method based on Fourier Transform micro-Raman spectroscopy coupled with Discriminant Analysis is here presented. This approach could be very useful for in-situ applications, such as customs inspections, since it drastically reduces time and costs of analysis., (Copyright © 2017. Published by Elsevier Ltd.)
- Published
- 2017
- Full Text
- View/download PDF
24. Controlling protected designation of origin of wine by Raman spectroscopy.
- Author
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Mandrile L, Zeppa G, Giovannozzi AM, and Rossi AM
- Subjects
- Discriminant Analysis, Food Storage standards, Geography, Italy, Magnetic Resonance Spectroscopy methods, Mass Spectrometry methods, Spectrum Analysis, Raman methods, Vitis chemistry, Wine analysis
- Abstract
In this paper, a Fourier Transform Raman spectroscopy method, to authenticate the provenience of wine, for food traceability applications was developed. In particular, due to the specific chemical fingerprint of the Raman spectrum, it was possible to discriminate different wines produced in the Piedmont area (North West Italy) in accordance with i) grape varieties, ii) production area and iii) ageing time. In order to create a consistent training set, more than 300 samples from tens of different producers were analyzed, and a chemometric treatment of raw spectra was applied. A discriminant analysis method was employed in the classification procedures, providing a classification capability (percentage of correct answers) of 90% for validation of grape analysis and geographical area provenance, and a classification capability of 84% for ageing time classification. The present methodology was applied successfully to raw materials without any preliminary treatment of the sample, providing a response in a very short time., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
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