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Automatic diagnosis techniques have evolved to identify age-related macular degeneration (AMD) by employing single modality Fundus images or optical coherence tomography (OCT). To classify ocular diseases, fundus and OCT images are the most crucial imaging modalities used in the clinical setting. Most deep learning-based techniques are established on a single imaging modality, which contemplates the ocular disorders to a specific extent and disregards other modality that comprises exhaustive information among distinct imaging modalities. This paper proposes a modality-specific multiscale color space embedding integrated with the attention mechanism based on transfer learning for classification (MCGAEc), which can efficiently extract the distinct modality information at various scales using the distinct color spaces. In this work, we first introduce the modality-specific multiscale color space encoder model, which includes diverse feature representations by integrating distinct characteristic color spaces on a multiscale into a unified framework. The extracted features from the prior encoder module are incorporated with the attention mechanism to extract the global features representation, which is integrated with the prior extracted features and transferred to the random forest classifier for the classification of AMD. To analyze the performance of the proposed MCGAEc method, a publicly available multi-modality dataset from Project Macula for AMD is utilized and compared with the existing models., Comment: 27th International Conference on Pattern Recognition (ICPR) 2024

Ultrasound is a widely used medical tool for non-invasive diagnosis, but its images often contain speckle noise which can lower their resolution and contrast-to-noise ratio. This can make it more difficult to extract, recognize, and analyze features in the images, as well as impair the accuracy of computer-assisted diagnostic techniques and the ability of doctors to interpret the images. Reducing speckle noise, therefore, is a crucial step in the preprocessing of ultrasound images. Researchers have proposed several speckle reduction methods, but no single method takes all relevant factors into account. In this paper, we compare seven such methods: Median, Gaussian, Bilateral, Average, Weiner, Anisotropic and Denoising auto-encoder without and with skip connections in terms of their ability to preserve features and edges while effectively reducing noise. In an experimental study, a convolutional noise-removing auto-encoder with skip connection, a deep learning method, was used to improve ultrasound images of breast cancer. This method involved adding speckle noise at various levels. The results of the deep learning method were compared to those of traditional image enhancement methods, and it was found that the proposed method was more effective. To assess the performance of these algorithms, we use three established evaluation metrics and present both filtered images and statistical data., Comment: Selected for presentation at 2024 IEEE South Asian Ultrasonics Symposium

Optical coherence tomography (OCT) is a medical imaging modality that allows us to probe deeper substructures of skin. The state-of-the-art wound care prediction and monitoring methods are based on visual evaluation and focus on surface information. However, research studies have shown that sub-surface information of the wound is critical for understanding the wound healing progression. This work demonstrated the use of OCT as an effective imaging tool for objective and non-invasive assessments of wound severity, the potential for healing, and healing progress by measuring the optical characteristics of skin components. We have demonstrated the efficacy of OCT in studying wound healing progress in vivo small animal models. Automated analysis of OCT datasets poses multiple challenges, such as limitations in the training dataset size, variation in data distribution induced by uncertainties in sample quality and experiment conditions. We have employed a U-Net-based model for the segmentation of skin layers based on OCT images and to study epithelial and regenerated tissue thickness wound closure dynamics and thus quantify the progression of wound healing. In the experimental evaluation of the OCT skin image datasets, we achieved the objective of skin layer segmentation with an average intersection over union (IOU) of 0.9234. The results have been corroborated using gold-standard histology images and co-validated using inputs from pathologists. Clinical Relevance: To monitor wound healing progression without disrupting the healing procedure by superficial, noninvasive means via the identification of pixel characteristics of individual layers., Comment: Accepted

Delay-and-sum (DAS) algorithms are widely used for beamforming in linear array photoacoustic imaging systems and are characterized by fast execution. However, these algorithms suffer from various drawbacks like low resolution, low contrast, high sidelobe artifacts and lack of visual coherence. More recently, adaptive weighting was introduced to improve the reconstruction image quality. Unfortunately, the existing state-of-the-art adaptive beamforming algorithms are computationally expensive and do not consider the specific noise characteristics of the acquired ultrasonic signal. In this article, we present a new adaptive weighting factor named the variational coherence factor (VCF), which takes into account the noise level variations of radio-frequency data. The proposed technique provides superior results in terms of image resolution, sidelobe reduction, signal-to-noise and contrast level improvement. The quantitative results of the numerical simulations and phantom imaging show that the proposed VCF assisted DAS method leads to 55% and 25% improvement in FWHM, 57% and 32% improvement in SNR, respectively, compared to the state-of-the-art DAS-based methods. The results demonstrate that the proposed method can effectively improve the reconstructed image quality and deliver satisfactory imaging performance even with a limited number of sensor elements. The proposed method can potentially reduce the instrumentation cost of the photoacoustic imaging system and contribute toward the clinical translation of the modality., Comment: Accepted for publications in IEEE Transactions on Instrumentation and Measurement

Objective:Optoacoustic (photoacoustic) tomography is aimed at reconstructing maps of the initial pressure rise induced by the absorption of light pulses in tissue. In practice, due to inaccurate assumptions in the forward model, noise and other experimental factors, the images are often afflicted by artifacts, occasionally manifested as negative values. The aim of the work is to develop an inversion method which reduces the occurrence of negative values and improves the quantitative performance of optoacoustic imaging. Methods: We present a novel method for optoacoustic tomography based on an entropy maximization algorithm, which uses logarithmic regularization for attaining non-negative reconstructions. The reconstruction image quality is further improved using structural prior based fluence correction. Results: We report the performance achieved by the entropy maximization scheme on numerical simulation, experimental phantoms and in-vivo samples. Conclusion: The proposed algorithm demonstrates superior reconstruction performance by delivering non-negative pixel values with no visible distortion of anatomical structures. Significance: Our method can enable quantitative optoacoustic imaging, and has the potential to improve pre-clinical and translational imaging applications., Comment: This article has been accepted for publication in IEEE Transactions on Biomedical Engineering (30 Dec 2018)

Visual observation of Cumulus Oocyte Complexes provides only limited information about its functional competence, whereas the molecular evaluations methods are cumbersome or costly. Image analysis of mammalian oocytes can provide attractive alternative to address this challenge. However, it is complex, given the huge number of oocytes under inspection and the subjective nature of the features inspected for identification. Supervised machine learning methods like random forest with annotations from expert biologists can make the analysis task standardized and reduces inter-subject variability. We present a semi-automatic framework for predicting the class an oocyte belongs to, based on multi-object parametric segmentation on the acquired microscopic image followed by a feature based classification using random forests., Comment: IEEE BHI 2016

Segmentation of biomedical images is essential for studying and characterizing anatomical structures, detection and evaluation of pathological tissues. Segmentation has been further shown to enhance the reconstruction performance in many tomographic imaging modalities by accounting for heterogeneities of the excitation field and tissue properties in the imaged region. This is particularly relevant in optoacoustic tomography, where discontinuities in the optical and acoustic tissue properties, if not properly accounted for, may result in deterioration of the imaging performance. Efficient segmentation of optoacoustic images is often hampered by the relatively low intrinsic contrast of large anatomical structures, which is further impaired by the limited angular coverage of some commonly employed tomographic imaging configurations. Herein, we analyze the performance of active contour models for boundary segmentation in cross-sectional optoacoustic tomography. The segmented mask is employed to construct a two compartment model for the acoustic and optical parameters of the imaged tissues, which is subsequently used to improve accuracy of the image reconstruction routines. The performance of the suggested segmentation and modeling approach are showcased in tissue-mimicking phantoms and small animal imaging experiments., Comment: Accepted for publication in IEEE Transactions on Medical Imaging

In this article, we present a novel scheme for segmenting the image boundary (with the background) in optoacoustic small animal in vivo imaging systems. The method utilizes a multiscale edge detection algorithm to generate a binary edge map. A scale dependent morphological operation is employed to clean spurious edges. Thereafter, an ellipse is fitted to the edge map through constrained parametric transformations and iterative goodness of fit calculations. The method delimits the tissue edges through the curve fitting model, which has shown high levels of accuracy. Thus, this method enables segmentation of optoacoutic images with minimal human intervention, by eliminating need of scale selection for multiscale processing and seed point determination for contour mapping., Comment: Engineering in Medicine and Biology Society (EMBC), 2015 37th Annual International Conference of the IEEE (Accepted version)