2,156 results on '"Man H"'
Search Results
2. Genome analysis and phylogenetic characterization of two deformed wing virus strains from Apis cerana in Vietnam
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Ha T. Thu, Nguyen T.K. Lien, Pham T. Lanh, Bui T.T. Duong, Nguyen T. Hoa, Man H. Phuoc, Pham H. Thai, and Dong Van Quyen
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Apis cerana ,Complete genome sequence ,Deformed wing virus ,Honeybee ,Iflavirus ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Background Deformed wing virus (DWV) is a virulent virus that causes honeybee disease. DWV can exist as a latent infection in honeybees, outbreak into epidemics, and cause serious damage to beekeeping cross the world, including Vietnam. Methods The two DWV strains circulating in Vietnamese honeybee, Apis cerana, were first isolated from adult honeybees in North Vietnam (DWV-NVN) and South Vietnam (DWV-SVN). Their complete nucleotide sequences were determined, aligned, and compared with other DWV strains. Results The two Vietnamese DWV strains comprised 10,113 bp and contained a large single open reading frame (ORF) of 2,893 amino acids, initiating at nucleotide 1,130 and terminating at nucleotide 9,812. Multiple nucleotide sequence alignment between these two DWV-VN strains and DWV strains in A. mellifera was performed. The DWV-VN strains showed a low genetic identity (from 91.4% to 92.0%) with almost of these strains, but lower identities (89.2% and 89.4%) with UK2 and (89.6%) with the China2 strain. Low identities (91.7% and 91.9%) were also observed between the China3 strain (in A. cerana) and the DWV-VN strains, respectively. The deduced amino acid sequence alignment showed high genetic similarities (97.0%–97.9%) when the USA1, Chile, Italy1, France, UK1, UK2, Japan, Korea2, China1, China2 and China3 strains were compared to the DWV-VN strains. This ratio was 96.7% and 96.8% when the Korea1 strain was compared to the DWV-SVN and DWV-NVN strains, respectively. Numerous amino acid substitutions were identified in the L, VP3, and RdRp sequences. Notably, we observed six substitutions positioned at amino acids 27 (E > I), 98 (S > T), 120 (A > V), 153 (M > T), 170 (D > F), and 174 (Y > F) in the L protein, two amino acid changes at positions 980 (S > A) and 1032 (E > T) in VP3, and one amino acid change at position 2627 (R > C) unique to the DWV-VN strains. Phylogenetic analysis based on complete genome sequences, RdRp sequences and Simplot analysis indicated that there was a significant difference between DWV-VN strains in A. cerana and DWV strains in A. mellifera. The results suggested that the genetic variations of the DWV-VN strains in A. cerana help them to adapt geographical conditions and may lead to change the viral pathogenicity of DWV-VN strains.
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- 2020
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3. A systematic review of evidence for fitness-to-drive among people with the mental health conditions of schizophrenia, stress/anxiety disorder, depression, personality disorder and obsessive compulsive disorder
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Carolyn A. Unsworth, Anne M. Baker, Man H. So, Priscilla Harries, and Desmond O’Neill
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Fitness-to-drive ,Mental health ,Systematic review ,Psychiatry ,RC435-571 - Abstract
Abstract Background Limited evidence exists regarding fitness-to-drive for people with the mental health conditions of schizophrenia, stress/anxiety disorder, depression, personality disorder and obsessive compulsive disorder (herein simply referred to as ‘mental health conditions’). The aim of this paper was to systematically search and classify all published studies regarding driving for this population, and then critically appraise papers addressing assessment of fitness-to-drive where the focus was not on the impact of medication on driving. Methods A systematic search of three databases (CINAHL, PSYCHINFO, EMBASE) was completed from inception to May 2016 to identify all articles on driving and mental health conditions. Papers meeting the eligibility criteria of including data relating to assessment of fitness-to-drive were critically appraised using the American Academy of Neurology and Centre for Evidence-Based Medicine protocols. Results A total of 58 articles met the inclusion criteria of driving among people with mental health conditions studied, and of these, 16 contained data and an explicit focus on assessment of fitness-to-drive. Assessment of fitness-to-drive was reported in three ways: 1) factors impacting on the ability to drive safely among people with mental health conditions, 2) capability and perception of health professionals assessing fitness-to-drive of people with mental health conditions, and 3) crash rates. The level of evidence of the published studies was low due to the absence of controls, and the inability to pool data from different diagnostic groups. Evidence supporting fitness-to-drive is conflicting. Conclusions There is a relatively small literature in the area of driving with mental health conditions, and the overall quality of studies examining fitness-to-drive is low. Large-scale longitudinal studies with age-matched controls are urgently needed in order to determine the effects of different conditions on fitness-to-drive.
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- 2017
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4. The Key Role of c-Fos for Immune Regulation and Bacterial Dissemination in Brucella Infected Macrophage
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Huynh T. Hop, Lauren T. Arayan, Tran X. N. Huy, Alisha W. B. Reyes, Son H. Vu, WonGi Min, Hu J. Lee, Man H. Rhee, Hong H. Chang, and Suk Kim
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Brucella abortus ,c-Fos ,MAPKs ,TLR-4 ,IL-10 ,Microbiology ,QR1-502 - Abstract
The cellular oncogene c-Fos (c-Fos) is a component of activator protein 1 (AP1), a master transcriptional regulator of cells. The suppression of c-Fos signaling by siRNA treatment resulted in significant induction of TLR4, which subsequently activates p38 and ERK1/2 mitogen-activated protein kinases (MAPKs) and enhances F-actin polymerization, leading to an increase in B. abortus phagocytosis. During B. abortus infection, c-Fos signaling is induced, which activates the downstream innate-immunity signaling cascade for bacterial clearance. The inhibition of c-Fos signaling led to increased production of interleukin 10 (IL-10), which partially suppressed lysosome-mediated killing, resulting in increased survival of B. abortus inside macrophages. We present evidence of the regulatory role played by the c-Fos pathway in proliferation during B. abortus infection; however, this was independent of the anti-Brucella effect of this pathway. Another finding is the essential contribution of c-Fos/TRAIL to infected-cell necrosis, which is a key event in bacterial dissemination. These data provide the mechanism via which c-Fos participates in host defense mechanisms against Brucella infection and in bacterial dissemination by macrophages.
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- 2018
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5. Activation of NF-kB-Mediated TNF-Induced Antimicrobial Immunity Is Required for the Efficient Brucella abortus Clearance in RAW 264.7 Cells
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Huynh T. Hop, Alisha W. B. Reyes, Tran X. N. Huy, Lauren T. Arayan, WonGi Min, Hu J. Lee, Man H. Rhee, Hong H. Chang, and Suk Kim
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B. abortus ,TNF ,TNFR-1 ,ROS ,NO ,NF-kB transcriptional factor ,Microbiology ,QR1-502 - Abstract
In this study, we explore the regulatory roles of pro-inflammatory cytokine tumor necrosis factor alpha (TNF) in the innate immunity of macrophages against B. abortus infection. We show that infection of macrophage with B. abortus induces marked expression and secretion of TNF which subsequently binds to TNF receptor 1 (TNFR-1) and activates a downstream signaling cascade of the innate immunity. Blocking of TNF signaling resulted in a notable increase of B. abortus survival which was associated with an increase of anti-inflammatory cytokine interleukin 10 (IL-10), a beneficial effector of Brucella survival, as well as remarkable decrease of reactive oxygen species (ROS) and nitric oxide (NO), antibrucella molecules. However, surprisingly, the interference of TNF did not show any influence on phagolysosome and cell death events. Furthermore, the transcriptional factor NF-kB was found to be a main mediator of TNF signaling when blocking of NF-kB pathway drastically suppressed the TNF-induced brucellacidal effect. Taken together, these findings clearly indicate that the immune cascade activated by TNF/TNFR-1 is required for the sufficient resistance to B. abortus survival in macrophages.
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- 2017
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6. Elements fondamentaux des documents de consentement a participer a la recherche sur le genome humain au Canada et de la Bibliotheque genomique humaine pancanadienne: orientation pour l'etablissement de politiques
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Longstaff, Holly, Flamenbaum, Jaime, Richer, Etienne, Egar, Jeanne, McMaster, Christopher R., and Zawati, Man H.
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Genomics -- Research ,Health - Abstract
La genomique humaine--l'etude de la totalite des genes d'une personne ou d'une population--est de plus en plus integree a la recherche et aux soins cliniques, y compris aux dossiers de [...]
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- 2022
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7. Core elements of participant consent documents for Canadian human genomics research and the National Human Genome Library: guidance for policy
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Longstaff, Holly, Flamenbaum, Jaime, Richer, Etienne, Egar, Jeanne, McMaster, Christopher R., and Zawati, Man H.
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Medical research -- Ethical aspects -- Standards ,Medicine, Experimental -- Ethical aspects -- Standards ,Genomics -- Ethical aspects -- Standards ,Genetic research -- Ethical aspects -- Standards ,Data entry -- Ethical aspects -- Standards ,Informed consent (Medical law) -- Standards ,Health - Abstract
Human genomics--the study of the entirety of a person's or population's genes--is increasingly being integrated into research and rapidly incorporated into clinical care, including into health records. (1,2) If most [...]
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- 2022
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8. An innovative seismo-petrophysical characterization of a turbidite sand in the Nam Con Son Basin, using permeability-linked adapted rock physics template (K-ARPT) with reference to gas exploration and carbon storage
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Giao, P. H., primary, Hien, D. H., additional, Trang, P. H., additional, Ngoc, P. Q., additional, Dung, B. V., additional, Man, H. Q., additional, and Phuong, B. T. N., additional
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- 2024
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9. Optimization of subcritical water pre-treatment for biogas enhancement on co-digestion of pineapple waste and cow dung using the response surface methodology
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Aili Hamzah, A.F., Hamzah, M.H., Mazlan, N.I., Che Man, H., Jamali, N.S., Siajam, S.I., and Show, P.L.
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- 2022
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10. Electronic structure of Ba(Zn0.875Mn0.125)2As2 studied by angle-resolved photoemission spectroscopy
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Zhu, Fengfeng, Jiang, W. X., Li, P., Wang, Z. Q., Man, H. Y., Li, Y. Y., Liu, Canhua, Guan, D. D., Jia, J. F., Ning, F. L., Luo, Weidong, and Qian, D.
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Condensed Matter - Materials Science - Abstract
Electronic structure of single crystalline Ba(Zn$_{0.875}$Mn$_{0.125}$)$_{2}$As$_{2}$, parent compound of the recently founded high-temperature ferromagnetic semiconductor, was studied by high-resolution photoemission spectroscopy (ARPES). Through systematically photon energy and polarization dependent measurements, the energy bands along the out-of-plane and in-plane directions were experimentally determined. Except the localized states of Mn, the measured band dispersions agree very well with the first-principle calculations of undoped BaZn$_{2}$As$_{2}$. A new feature related to Mn 3d states was identified at the binding energies of about -1.6 eV besides the previously observed feature at about -3.3 eV. We suggest that the hybridization between Mn and As orbitals strongly enhanced the density of states around -1.6 eV. Although our resolution is much better compared with previous soft X-ray photoemission experiments, no clear hybridization gap between Mn 3d states and the valence bands proposed by previous model calculations was detected.
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- 2016
11. Residual-based a posteriori error estimates for symmetric conforming mixed finite elements for linear elasticity problems
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Chen, L, Hu, J, Huang, X, and Man, H
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symmetric mixed finite element ,linear elasticity problems ,a posteriori error estimator ,adaptive method ,math.NA ,General Mathematics ,Pure Mathematics - Abstract
A posteriori error estimators for the symmetric mixed finite element methods for linear elasticity problems with Dirichlet and mixed boundary conditions are proposed. Reliability and efficiency of the estimators are proved. Finally, numerical examples are presented to verify the theoretical results.
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- 2018
12. Dynamic anaerobic membrane bioreactor (DAnMBR) with phase separation for food processing wastewater treatment at mesophilic temperature: Characterization of cake layer
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Mahat, S.B., Omar, R., Che Man, H., Md Idris, A.I., Mustapa Kamal, S.M., Abdullah, L.C., and Shreeshivadasan, C.
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- 2021
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13. Direct observation of magnon-phonon coupling in yttrium iron garnet
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Man, H, Shi, Z, Xu, G, Xu, Y, Chen, X, Sullivan, S, Zhou, J, Xia, K, Shi, J, and Dai, P
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cond-mat.str-el - Abstract
The magnetic insulator yttrium iron garnet (YIG) with a ferrimagnetic transition temperature of ∼560 K has been widely used in microwave and spintronic devices. Anomalous features in spin Seeback effect (SSE) voltages have been observed in Pt/YIG and attributed to magnon-phonon coupling. Here, we use inelastic neutron scattering to map out low-energy spin waves and acoustic phonons of YIG at 100 K as a function of increasing magnetic field. By comparing the zero and 9.1 T data, we find that instead of splitting and opening up gaps at the spin wave and acoustic phonon dispersion intersecting points, magnon-phonon coupling in YIG enhances the hybridized scattering intensity. These results are different from expectations of conventional spin-lattice coupling, calling for different paradigms to understand the scattering process of magnon-phonon interactions and the resulting magnon polarons.
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- 2017
14. Unlocking the potential of RNA-based therapeutics in the lung: current status and future directions
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Man, H. S. Jeffrey, primary, Moosa, Vaneeza A., additional, Singh, Anand, additional, Wu, Licun, additional, Granton, John T., additional, Juvet, Stephen C., additional, Hoang, Chuong D., additional, and de Perrot, Marc, additional
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- 2023
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15. Simultaneous Observation of the Inner and Outer Electron Diffusion Region in Reconnection with Large Guide Field
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Tian, Z. C., primary, Zhou, M., additional, Man, H. Y., additional, Zhong, Z. H., additional, Deng, X. H., additional, Gershman, D. J., additional, Khotyaintsev, Y. V., additional, and Russell, C. T., additional
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- 2023
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16. Angiogenic patterning by STEEL, an endothelial-enriched long noncoding RNA
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Man, H. S. Jeffrey, Sukumar, Aravin N., Lam, Gabrielle C., Turgeon, Paul J., Yan, Matthew S., Ku, Kyung Ha, Dubinsky, Michelle K., Ho, J. J. David, Wang, Jenny Jing, Das, Sunit, Mitchell, Nora, Oettgen, Peter, Sefton, Michael V., and Marsden, Philip A.
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- 2018
17. Spin-dependent Quantum Interference in Single-Wall Carbon Nanotubes with Ferromagnetic Contacts
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Man, H. T., Wever, I. J. W., and Morpurgo, A. F.
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Condensed Matter - Mesoscale and Nanoscale Physics - Abstract
We report the experimental observation of spin-induced magnetoresistance in single-wall carbon nanotubes contacted with high-transparency ferromagnetic electrodes. In the linear regime the spin-induced magnetoresistance oscillates with gate voltage in quantitative agreement with calculations based on a Landauer-Buttiker model for independent electrons. Consistent with this interpretation, we find evidence for bias-induced oscillation in the spin-induced magnetoresistance signal on the scale of the level spacing in the nanotube. At higher bias, the spin-induced magnetoresistance disappears because of a sharp decrease in the effective spin-polarization injected from the ferromagnetic electrodes., Comment: Replaced with published version
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- 2005
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18. Transport through a superconductor-interacting normal metal junction: a phenomenological description
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Man, H. T., Klapwijk, T. M., and Morpurgo, A. F.
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Condensed Matter - Superconductivity ,Condensed Matter - Mesoscale and Nanoscale Physics - Abstract
We propose a phenomenological description of electronic transport through a normal metal/superconductor interface of arbitrary transparency, which accounts for the presence of electron-electron interaction in the normal metal. The effect of interactions is included through an energy-dependent transmission probability that is inserted in the expression for the current-voltage characteristics of a non-interacting system. This results in a crossover from the Andreev to the tunneling limit as a function of the energy at which transport is probed. The proposed description reproduces qualitatively the results obtained with formally correct theories as well as experimental observations. In view of its simplicity, we expect our approach to be of use for the interpretation of future experiments.
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- 2005
19. Sample-specific and Ensemble-averaged Magnetoconductance of Individual Single-Wall Carbon Nanotubes
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Man, H. T. and Morpurgo, A. F.
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Condensed Matter - Mesoscale and Nanoscale Physics - Abstract
We discuss magnetotransport measurements on individual single-wall carbon nanotubes with low contact resistance, performed as a function of temperature and gate voltage. We find that the application of a magnetic field perpendicular to the tube axis results in a large magnetoconductance of the order of e^2/h at low temperature. We demonstrate that this magnetoconductance consists of a sample-specific and of an ensemble-averaged contribution, both of which decrease with increasing temperature. The observed behavior resembles very closely the behavior of more conventional multi-channel mesoscopic wires, exhibiting universal conductance fluctuations and weak localization. A theoretical analysis of our experiments will enable to reach a deeper understanding of phase-coherent one-dimensional electronic motion in SWNTs., Comment: Replaced with published version. Minor changes in text
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- 2004
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20. Unlocking the potential of RNA-based therapeutics in the lung: current status and future directions.
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Jeffrey Man, H. S., Moosa, Vaneeza A., Singh, Anand, Licun Wu, Granton, John T., Juvet, Stephen C., Hoang, Chuong D., and de Perrot, Marc
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LUNGS ,COVID-19 pandemic ,NON-coding RNA ,LUNG diseases ,COVID-19 vaccines ,NANOPARTICLES - Abstract
Awareness of RNA-based therapies has increased after the widespread adoption of mRNA vaccines against SARS-CoV-2 during the COVID-19 pandemic. These mRNA vaccines had a significant impact on reducing lung disease and mortality. They highlighted the potential for rapid development of RNA-based therapies and advances in nanoparticle delivery systems. Along with the rapid advancement in RNA biology, including the description of noncoding RNAs as major products of the genome, this success presents an opportunity to highlight the potential of RNA as a therapeutic modality. Here, we review the expanding compendium of RNAbased therapies, their mechanisms of action and examples of application in the lung. The airways provide a convenient conduit for drug delivery to the lungs with decreased systemic exposure. This review will also describe other delivery methods, including local delivery to the pleura and delivery vehicles that can target the lung after systemic administration, each providing access options that are advantageous for a specific application. We present clinical trials of RNA-based therapy in lung disease and potential areas for future directions. This review aims to provide an overview that will bring together researchers and clinicians to advance this burgeoning field. [ABSTRACT FROM AUTHOR]
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- 2023
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21. Mechanical Ventilation, Conventional
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Man, H. S. Jeffrey, Papadakos, Peter J., editor, and Gestring, Mark L., editor
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- 2015
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22. Prognostic Value of EMT Gene Signature in Malignant Mesothelioma
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Wu, Licun, primary, Yoshihara, Kosuke, additional, Yun, Hana, additional, Karim, Saraf, additional, Shokri, Nastaran, additional, Zaeimi, Fatemeh, additional, Man, H. S. Jeffrey, additional, Zia, Amin, additional, Felley-Bosco, Emanuela, additional, and de Perrot, Marc, additional
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- 2023
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23. Gastrointestinal, influenza-like illness and dermatological complaints following exposure to floodwater : a cross-sectional survey in The Netherlands
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DE MAN, H., GRAS, L. MUGHINI, SCHIMMER, B., FRIESEMA, I. H. M., DE RODA HUSMAN, A. M., and VAN PELT, W.
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- 2016
24. DEM parameters of slope cultivated purple soil in Southwest China and interaction mechanism between very narrow tine
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Shoutai Li, Man Hu, Yan Liu, and Yunwu Li
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DEM parameters ,Slope cultivated soil ,Purple soil ,Static-dynamic repose angle ,Very narrow tine ,Interaction mechanism ,Medicine ,Science - Abstract
Abstract The establishment of discrete element method (DEM) model for simulating the behavior of viscous soil can significantly contribute to the development of agricultural machinery, particularly in hilly areas worldwide. There is limited research on the parameter calibration of the DEM for purple soil in the Southwest China and its interaction mechanism between very narrow tine. Piling up test, rotating drum test and design-expert 12.0 software were used to design Plackett–Burman test, steepest climb test and Box–Behnken test successively. Combined with soil intrinsic parameter measurement test, soil simulation parameter calibration and optimization were completed. Based on the optimized DEM simulation parameters, the average relative errors of static repose angle and dynamic repose angle were 1.934 and 2.289%, respectively. The accuracy of parameter calibration results of DEM simulation was proved. Three kinds of very narrow tines were designed and processed. The interaction model and mechanism between soil-touch parts and soil was established. The average relative errors between the DEM simulation results of the forward resistance and disturbance area of the three soil-touch parts and the soil bin test results were 7.22% and 11.24%, respectively, which further proved that the calibration results of DEM parameters of purple soil and the interaction model with the soil-touch parts in this study were reliable. The physical and interaction parameters of slope cultivated purple soil in Southwest China were determined based on static-dynamic repose angle, providing more accurate parameters for study of soil-touch parts.
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- 2024
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25. Impact of COVID-19 on Dental Students’ Mental Health Status and Perception of SARS-CoV-2 Vaccine
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Man Hung, Nicole Hablitzel, Sharon Su, Samantha Melnitsky, and Amir Mohajeri
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COVID-19 ,vaccine ,dental student ,mental health ,Specialties of internal medicine ,RC581-951 - Abstract
The COVID-19 pandemic disrupted dental education significantly, forcing adaptations in both didactic and clinical curricula. This study evaluates the impact of COVID-19 on dental students’ mental health and perceptions of the SARS-CoV-2 vaccine. An anonymous online survey was administered to dental students at Roseman University of Health Sciences, focusing on health experiences and vaccination perceptions. Results showed 56.8% of students were concerned about their emotional health, 82.1% felt stressed, and 60.6% felt depressed. About 81.9% received the vaccine, with 75.5% believing it effective, though only 55.3% supported mandatory vaccination. The pandemic negatively impacted students’ emotional health, indicating a need for institutional mental health support. This study was conducted during the COVID-19 pandemic, and findings relate specifically to that period. Further research can focus on investigation of reasonings behind the sentiments.
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- 2024
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26. Red ginseng extract inhibits lipopolysaccharide-induced platelet–leukocyte aggregates in mice
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Yuan Yee Lee, Sung Dae Kim, Jin-Kyu Park, Won-Jae Lee, Jee Eun Han, Min-Soo Seo, Min-Goo Seo, Seulgi Bae, Dongmi Kwak, Evelyn Saba, and Man Hee Rhee
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Platelet activation ,Platelet–leukocyte aggregates ,Red ginseng extract ,Inflammation ,Thrombosis ,Botany ,QK1-989 - Abstract
Background: Platelet–leukocyte aggregates (PLAs) play important roles in cardiovascular disease and sepsis. Red ginseng extract (RGE) has been well-studied for its antiplatelet and anti-inflammatory activities. However, the potential inhibitory effects of RGE on PLA have not been investigated. Methods: Six-week-old ICR mice were given oral gavage of RGE for 7 days, followed by an intraperitoneal injection of 15 mg/kg of lipopolysaccharide. Mice were euthanized 24 h later, and blood samples were collected for further analysis. Flow cytometry was utilized to sort populations of PLAs and platelet–neutrophil aggregates (PNAs). By using confocal microscopy, PNAs were validated. Morphological changes in platelets and leukocytes were visualized with scanning electron microscopy. Expressions of tissue factor (TF) and platelet factor 4 (PF4) were investigated using enzyme-linked immunosorbent assay. Results: Populations of activated platelets, PLAs and PNAs, were significantly increased with LPS-induction. Treatment with 200 and 400 mg/kg of RGE decreased platelet activation. Moreover, the populations of PLAs and PNAs were reduced. PNAs were visible in the blood of septic mice, and this was attenuated by treatment with 400 mg/kg of RGE. Morphologically, sepsisinduced platelet activation and fibrin formation in the blood. This was reduced with RGE treatment. Sepsis-induced increase in the plasma levels of TF and PF4 was also reduced with RGE treatment. Conclusion: This study shows that RGE is a potential therapeutic that reduces the activation of platelets and targets PLA and PNA formation. Detailed inhibitory mechanisms of RGE should be studied.
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- 2024
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27. Genetic causal association between gut microbiota and sepsis: Evidence from a two-sample bidirectional Mendelian randomization analysis
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Jungen Tang and Man Huang
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Gut microbiota ,Sepsis ,Mendelian randomization ,Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
Background: Sepsis is a severe and potentially life-threatening condition characterized by a dysregulated host response and organ dysfunction. The causal relationship between intestinal microbiota and sepsis is unclear. Methods: A two-sample Mendelian randomization (MR) study was performed to proxy the causal association between gut microbiota and sepsis. The genome-wide association study (GWAS) data of sepsis and gut microbiome were collected from the Integrative Epidemiology Unit (IEU) OpenGWAS, with summary-level data obtained from the UK Biobank. Five traditional methods were used to estimate the potential causal relationships between gut microbiota and sepsis, including the inverse-variance weighted method, weighted median method, MR-Egger regression, simple mode, and weighted mode. Reverse MR analysis was performed on the bacteria that were found to be causally associated with sepsis in forward MR analysis. Cochran's Q statistic was used to quantify the heterogeneity of instrumental variables. Results: The inverse-variance weighted estimate suggested that class Lentisphaeria (odds ratio [OR]=0.86, 95% confidence interval [CI]: 0.78 to 0.94, P=0.0017, q=0.1596) and order Victivallales (OR=0.86, 95% CI: 0.78 to 0.94, P=0.0017, q=0.1596) have a protective effect on sepsis. The genus Eubacterium eligens group (OR=1.34, 95% CI: 1.11 to 1.63, P=0.0029, q=0.1881) was positively associated with the risk of sepsis. Sepsis may be a significant risk factor for genus Odoribacter (OR=1.18, 95% CI: 1.10 to 1.39, P=0.0415, q=0.9849) and Phascolarctobacterium (OR=1.21, 95% CI: 1.00 to 1.46, P=0.0471, q=0.9849), but this effect was not statistically significant after false discovery rate correction. There was a suggestive association between sepsis and Faecalibacterium (OR=0.85, 95% CI: 0.73 to 0.98, P=0.0278) and Ruminococcus 1 (OR=0.85, 95% CI: 0.73 to 1.00, P=0.0439), which were not significant after false discovery rate correction (q>0.2). Conclusions: This study found that class Lentisphaeria, order Victivallales, and genus Eubacterium eligens group may have a causal relationship with the risk of sepsis.
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- 2024
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28. Adaptation of quadtree meshes in the scaled boundary finite element method for crack propagation modelling
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Ooi, E.T., Man, H., Natarajan, S., and Song, C.
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- 2015
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29. Final 5‐Year Report of the Randomized BIO‐RESORT Trial Comparing 3 Contemporary Drug‐Eluting Stents in All‐Comers
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Ploumen, Eline H., primary, Pinxterhuis, Tineke H., additional, Buiten, Rosaly A., additional, Zocca, Paolo, additional, Danse, Peter W., additional, Schotborgh, Carl E., additional, Scholte, Martijn, additional, Gin, R. Melvyn Tjon Joe, additional, Somi, Samer, additional, van Houwelingen, K. Gert, additional, Stoel, Martin G., additional, de Man, H. A. F., additional, Hartmann, Marc, additional, Linssen, Gerard C. M., additional, van der Heijden, Liefke C., additional, Kok, Marlies M., additional, Doggen, Carine J. M., additional, and von Birgelen, Clemens, additional
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- 2022
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30. 18F-AlF-NOTA-PCP1 PET/CT Imaging Visualize Radiation-Induced PD-L1 Expression in Glioblastoma Subcutaneous Xenograft Mouse Models
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Chen, Y., primary, Liu, Z., additional, Zhang, Y., additional, Man, H., additional, and Yu, J., additional
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- 2022
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31. ODP028 Association Between Serum Steroid Profile and Metabolic Risks in Adults with Classic Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency
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Ahn, Chang H, primary, Shim, Jaeyoon, additional, Jang, Han N, additional, Lee, Young A, additional, Choi, Man H, additional, and Kim, Jung Hee, additional
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- 2022
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32. Foaming Tendency in Anaerobic Digestion of Wastewater Inoculated with Anaerobic Sludge and Cow Manure: Effect of Protein Concentration
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Syed Sabeer Ali, S N, primary, Omar, R, additional, Che Man, H, additional, Md Idris, A I, additional, and Tajuddin, H A, additional
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- 2022
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33. Combined Bacterial Meningitis and Infective Endocarditis: When Should We Search for the Other When Either One is Diagnosed?
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Béraud, Guillaume, Tubiana, Sarah, Erpelding, Marie-Line, Le Moing, Vincent, Chirouze, Catherine, Gorenne, Isabelle, Manchon, Pauline, Tattevin, Pierre, Vernet, Veronique, Varon, Emmanuelle, Hoen, Bruno, Duval, Xavier, Obadia, J., Leport, C., Poyart, C., Revest, M., Selton-Suty, C., Strady, C., Vandenesch, F., Bernard, Y., Chocron, S., Plesiat, P., Abouliatim, I., de Place, C., Donnio, P., Alla, F., Carteaux, J., Doco-Lecompte, T., Lion, C., Aissa, N., Baehrel, B., Jaussaud, R., Nazeyrollas, P., Cambau, E., Iung, B., Nataf, P., Chidiac, C., Celard, M., Delahaye, F., Aumaître, H., Frappier, J., Oziol, E., Sotto, A., Sportouch, C., Bouvet, A., Bes, M., Abassade, P., Abrial, E., Acar, C., Alexandra, J., Amireche, N., Amrein, D., Andre, P., Appriou, M., Arnould, M., Atoui, A., Aziza, F., Baille, N., Bajolle, N., Battistella, P., Baumard, S., Ben Ali, A., Bertrand, J., Bialek, S., Bois Grosse, M., Boixados, M., Borlot, F., Bouchachi, A., Bouche, O., Bouchemal, S., Bourdon, J., Brasme, L., Bruntz, J., Cailhol, J., Caplan, M.P., Carette, B., Cartry, O., Cazorla, C., Chamagne, H., Champagne, H., Chanques, G., Chevalier, B., Chometon, F., Christophe, C., Colin de Verdiere, N., Daneluzzi, V., David, L., Danchin, N., de Lentdecker, P., Delcey, V., Deleuze, P., Deroure, B., Descotes-Genon, V., Didier Petit, K., Dinh, A., Doat, V., Duchene, F., Duhoux, F., Dupont, M., Ederhy, S., Epaulard, O., Evest, M., Faucher, J., Fauveau, E., Ferry, T., Fillod, M., Floch, T., Fraisse, T., Frapier, J., Freysz, L., Fumery, B., Gachot, B., Gallien, S., Garcon, P., Gaubert, A., Genoud, J., Ghiglione, S., Godreuil, C., Gandjbakhch, I., Grentzinger, A., Groben, L., Gherissi, D., Hagege, A., Hammoudi, N., Heliot, F., Henry, P., Houriez, P., Hustache-Mathieu, L., Huttin, O., Imbert, S., Jaureguiberry, S., Kaaki, M., Konate, A., Kuhn, J., Kural Menasche, S., Lafitte, A., Lafon, B., Lanternier, F., Le Chenault, V., Lechiche, C., Lefevre Thibaut, S., Lefort, A., Lemoine, J., Lepage, L., Lepousé, C., Leroy, J., Lesprit, P., Letranchant, L., Loncar, G., Lorentz, C., Magnin-Poull, I., Makinson, A., Man, H., Mansouri, M., Marçon, O., Maroni, J., Masse, V., Maurier, F., Mechaï, F., Merceron, O., Messika-Zeitoun, D., Metref, Z., Meyssonnier, V., Mezher, C., Micheli, S., Monsigny, M., Mouly, S., Mourvillier, B., Nallet, O., Nazerollas, P., Noel, V., Payet, B., Pelletier, A., Perez, P., Petit, J., Philippart, F., Piet, E., Plainvert, C., Popovic, B., Porte, J., Pradier, P., Ramadan, R., Richemond, J., Rodermann, M., Roncato, M., Roigt, I., Ruyer, O., Saada, M., Schwartz, J., Simon, M., Simorre, B., Skalli, S., Spatz, F., Sudrial, J., Tartiere, L., Terrier de La Chaize, A., Thiercelin, M., Thomas, D., Thomas, M., Toko, L., Tournoux, F., Tristan, A., Trouillet, J., Tual, L., Verdier, F., Vernet Garnier, V., Vidal, V., Weyne, P., Wolff, M., Wynckel, A., Zannad, N., Zinzius, P., Ploy, M.-C., Caron, F., Bollaert, P.-E., Gaillot, O., Taha, M.-K., Bonacorsi, S., Lecuit, M., Gravet, A., Frachet, B., Debroucker, T., Levy-Bruhl, D., Raffi, F., Preau, M., Anguel, N., Argaud, L., Arista, S., Armand-Lefevre, L., Balavoine, S., Baraduc, R., Barnaud, G., Bernard, L., Bernars, G., Bertei, D., Bessede, E., Billard Pomares, T., Biron, C., Bland, S., Boileau, J., Boubeau, P., Bourdon, S., Bousquet, A., Boyer, S., Bozorg-Grayeli, A., Bret, L., Bretonniere, C., Bricaire, F., Brocas, E., Brun, M., Buret, J., Burucoa, C., Cabalion, J., Cabon, M., Camuset, G., Canevet, C., Carricajo, A., Castan, B., Caumes, E., Cazanave, C., Chabrol, A., Challan-Belval, T., Chanteperdrix-Marillier, V., Chaplain, C., Charlier-Woerther, C., Chaussade, H., Clair, B., Colot, J., Conil, J.-M., Cordel, H., Cormier, P., Cousson, J., Cronier, P., Cua, E., Dao-Dubremetz, A., Dargere, S., Degand, N., Dekeyser, S., Delaune, D., Denes, E., Dequin, P.-F., Descamps, D., Descloux, E., Desmaretz, J.-L., Diehl, J.-L., Dimet, J., Escaut, L., Fabe, C., Faibis, F., Flateau, C., Fonsale, N., Forestier, E., Fortineau, N., Gagneux-Brunon, A., Garandeau, C., Garcia, M., Garot, D., Gaudry, S., Goehringer, F., Gregoire-Faucher, V., Grosset, M., Gubavu, C., Gueit, I., Guelon, D., Guimard, T., Guinard, J., Hadou, T., Helene, J.-P., Henard, S., Henry, B., Hochart, A.-C., Illes, G., Jaffuel, S., Jarrin, I., Jaureguy, F., Joseph, C., Juvin, M.-E., Kayal, S., Kerneis, S., Lacassin, F., Lamaury, I., Lanotte, P., Laurens, E., Laurichesse, H., Le Brun, C., Le Turnier, P., Lecuyer, H., Ledru, S., Legrix, C., Lemaignen, A., Lemble, C., Lemee, L., Lesens, O., Levast, M., Lhommet, C., Males, S., Malpote, E., Martin-Blondel, G., Marx, M., Masson, R., Matray, O., Mbadi, A., Mellon, G., Merens, A., Meyohas, M.-C., Michon, A., Mootien Yoganaden, J., Morquin, D., Mrozek, N., Nguyen, S., Nguyen, Y., Ogielska, M., Page, B., Patrat-Delon, S., Patry, I., Pechinot, A., Picot, S., Pierrejean, D., Piroth, L., Plassart, C., Plessis, P., Portel, L., Poubeau, P., Poupard, M., Prazuck, T., Quaesaet, L., Ramanantsoa, A., Rapp, C., Raskine, L., Raymond, J., Riche, A., Robaday-Voisin, S., Robin, F., Romaszko, J.-P., Rousseau, F., Roux, A.-L., Royer, C., Salmon, D., Saroufim, C., Schmit, J.-L., Sebire, M., Segonds, C., Sivadon-Tardy, V., Soismier, N., Son, O., Sunder, S., Suy, F., Tande, D., Tankovic, J., Valin, N., van Grunderbeeck, N., Verdon, R., Vergnaud, M., Vernet-Garnier, V., Vidal, M., Vitrat, V., Vittecoq, D., Vuotto, F., Laouenan, C., Marcault, E., Mentre, F., Pasquet, B., Roy, C., Centre hospitalier universitaire de Poitiers (CHU Poitiers), Centre d'investigation Clinique [CHU Bichat] - Épidémiologie clinique (CIC 1425), AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, Centre d'investigation clinique [Nancy] (CIC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), CHU Pontchaillou [Rennes], ARN régulateurs bactériens et médecine (BRM), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Université Sorbonne Paris Nord, Centre d'investigation clinique - Epidémiologie clinique [Nancy] (CIC-EC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), CHU Montpellier, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre Hospitalier Universitaire de Reims (CHU Reims), Centre Hospitalier Intercommunal de Créteil (CHIC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and The AEPEI IE cohort was funded by a research grant from the French Ministry of Health (PHRC 2007), grants from the Société Française de Cardiologie, the European Society of Clinical Microbiology and Infectious Diseases, and Novartis France. The sponsor was Délégation à la Recherche Clinique et au Développement, Centre Hospitalier Universitaire de Besançon. The COMBAT cohort was funded by Assistance Publique—Hôpitaux de Paris, Inserm, The French Society of Infectious Diseases (SPILF), and Pfizer Laboratory. It was also supported by the Observatoire de la Resistance du Pneumocoque (ORP) and Santé Publique France. The sponsor of the study and the funding sources had no role in study design, data collection, data analysis, data interpretation or writing of the report. The corresponding author had full access to all the data in the study and had final responsibility for the decision to submit it for publication. The Rapid Service Fee was funded by the University Hospital of Poitiers, to which the corresponding author is affiliated.
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Microbiology (medical) ,Infectious Diseases ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Echocardiography ,Austrian syndrome ,Staphylococcus ,[SDV]Life Sciences [q-bio] ,Bacterial meningitis ,Streptococcus ,Infective endocarditis - Abstract
International audience; Introduction: We aimed to describe patients with coexisting infective endocarditis (IE) and bacterial meningitis (BM).Methods: We merged two large prospective cohorts, an IE cohort and a BM cohort, with only cases of definite IE and community-acquired meningitis. We compared patients who had IE and BM concurrently to patients with IE only and BM only.Results: Among the 1030 included patients, we identified 42 patients with IE-BM (4.1%). Baseline characteristics of patients with IE-BM were mostly similar to those of patients with IE, but meningitis was the predominant presentation at admission (39/42, 92.3%). Causative pathogens were predominantly Streptococcus pneumoniae (18/42, 42.9%) and Staphylococcus aureus (14/42, 33.3%). All pneumococcal IE were associated with BM (18/18). BM due to oral and group D streptococci, Streptococcus agalactiae, and S. aureus were frequently associated with IE (14/30, 46.7%). Three-month mortality was 28.6% in patients with IE-BM, 20.5% in patients with IE, and 16.6% in patients with BM.Conclusions: Patients with pneumococcal IE or altered mental status during IE must be investigated for BM. Patients with S. aureus, oral and group D streptococcal or enterococcal BM, or unfavorable outcome in pneumococcal meningitis would benefit from an echocardiography. Patients with the dual infection have the worst prognosis. Their identification is mandatory to initiate appropriate treatment.
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- 2022
34. Effect of Laser Acupuncture Therapy on Bone and Articular Cartilage under Simulated Microgravity
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Wang, Q., Guo, X., Wang, X. Y., Liu, M. Q., Zhang, M., Zheng, Y. P., Man, H. C., Magjarevic, Ratko, editor, Lim, C. T., editor, and Goh, J. C. H., editor
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- 2010
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35. Health risk assessment for splash parks that use rainwater as source water
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de Man, H., Bouwknegt, M., van Heijnsbergen, E., Leenen, E.J.T.M., van Knapen, F., and de Roda Husman, A.M.
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- 2014
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36. Human exposure to endotoxins and fecal indicators originating from water features
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de Man, H., Heederik, D.D.J., Leenen, E.J.T.M., de Roda Husman, A.M., Spithoven, J.J.G., and van Knapen, F.
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- 2014
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37. Quantitative assessment of infection risk from exposure to waterborne pathogens in urban floodwater
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de Man, H., van den Berg, H.H.J.L., Leenen, E.J.T.M., Schijven, J.F., Schets, F.M., van der Vliet, J.C., van Knapen, F., and de Roda Husman, A.M.
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- 2014
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38. Fracture of Foamed Cementitious Materials: A Combined Experimental and Numerical Study
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Meyer, D., Man, H. -K., van Mier, Jan G. M., Gladwell, G. M. L., editor, Moreau, R., editor, Zhao, Han, editor, and Fleck, N. A., editor
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- 2009
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39. Three Dimensional Interactions between Solar Wind and Magnetopause
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Darvish, Y., Far man, H., Zhuang, F. G., editor, and Li, J. C., editor
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- 2009
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40. Gender-specific alteration of steroid metabolism and its impact on viral replication in a mouse model of hepatitis B virus infection
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Eun-Sook Park, Juhee Won, Sung Hyun Ahn, Ah Ram Lee, Donghyo Lee, Ju-Yeon Moon, Man Ho Choi, and Kyun-Hwan Kim
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Hepatitis B virus (HBV) ,dihydrotestosterone ,steroid profiling ,gender-specific alteration ,androgens ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Hepatitis B virus (HBV) is a sex-specific pathogen that is more severe in males than in females. Sex disparities in HBV infection have been attributed to hormonal differences between males and females. However, whether HBV infection affects the metabolic signatures of steroid hormones and how these influences viral replication remains unclear. In this study, we investigated whether HBV infection alters steroid metabolism and its effects on HBV replication. Serum samples from male and female mice obtained after the hydrodynamic injection of replication-competent HBV plasmids were subjected to quantitative steroid profiling. Serum steroid levels in mice were analyzed using an in vitro metabolism assay with the mouse liver S9 fraction. The alteration of steroids by HBV infection was observed only in male mice, particularly with significant changes in androgens, whereas no significant hormonal changes were observed in female mice. Among the altered steroids, dehydroepiandrosterone (DHEA) levels increased the most in male mice after HBV infection. An in vitro metabolism assay revealed that androgen levels were significantly reduced in HBV-infected male mice. Furthermore, the genes involved in DHEA biosynthesis were significantly upregulated in HBV-infected male mice. Interestingly, reduced dihydrotestosterone in male mice significantly inhibits viral replication by suppressing HBV promoter activity, suggesting a viral strategy to overcome the antiviral effects of steroid hormones in males. Our data demonstrated that HBV infection can cause sex-specific changes in steroid metabolism.
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- 2024
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41. Long noncoding RNA GATA2-AS1 augments endothelial hypoxia inducible factor 1-α induction and regulates hypoxic signaling.
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Man, H. S. Jeffrey, Subramaniam, Noeline, Downs, Tiana, Sukumar, Aravin N., Saha, Aninda D., Nair, Ranju, Chen, Lucy, Teitelbaum, Daniel, Turgeon, Paul J., Kyung Ha Ku, Tran, Eileen, de Perrot, Marc, and Marsden, Philip A.
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- *
LINCRNA , *HYPOXIA-inducible factor 1 , *SINGLE nucleotide polymorphisms , *VASCULAR endothelial cells , *HYPOXEMIA , *VASCULAR endothelium , *CORONARY artery disease - Abstract
Vascular endothelial cells form the inner cellular lining of blood vessels and have myriad physiologic functions including angiogenesis and response to hypoxia. We recently identified a set of endothelial cell (EC)-enriched long noncoding RNAs (lncRNAs) in differentiated human primary cell types and described the role of the STEEL lncRNA in angiogenic patterning. We sought to further understand the role of EC-enriched lncRNAs in physiologic adaptation of the vascular endothelium. In this work, we describe an abundant, cytoplasmic, andEC-enriched lncRNA,GATA2-AS1, that is divergently transcribed from the EC-enriched developmental regulator, GATA2. While GATA2-AS1 is largely coexpressedwithGATA2in ECs,GATA2-AS1andGATA2appear to be complementary rather than synergistic as they havemostly distinct target genes. Common single nucleotide variants in GATA2-AS1 exons are associated with early-onset coronary artery disease and decreased expression of GATA2-AS1 in endothelial cell lines. In most cells, HIF1-a is central to the transcriptional response to hypoxia, while in ECs, both HIF1-a and HIF2-a are required to coordinate an acute and chronic response, respectively. In this setting, GATA2-AS1 contributes to the "HIF switch" and augments HIF1-a induction in acute hypoxia to regulate HIF1-a/HIF2-a balance. In hypoxia, GATA2-AS1 orchestrates HIF1-a-dependent induction of the glycolytic pathway and HIF1-a-independent maintenance of mitochondrial biogenesis. Similarly, GATA2-AS1 coordinates both metabolism and "tip/stalk" cell signaling to regulate angiogenesis in hypoxic ECs. Furthermore, we find that GATA2-AS1 expression patterns are perturbed in atherosclerotic disease. Together, these results define a role for GATA2-AS1 in the EC-specific response to hypoxia. [ABSTRACT FROM AUTHOR]
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- 2023
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42. In Situ Observation of Magnetic Reconnection Between an Earthward Propagating Flux Rope and the Geomagnetic Field
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Man, H. Y, Zhou, M, Deng, X. H, Fu, H. S, Zhong, Z. H, Chen, Z. Z, Russell, C. T, Strangeway, R. J, Paterson, W. R, Giles, B. L, Lindqvist, P.-A, Ergun, R. E, and Burch, J. L
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Plasma Physics - Abstract
It has been proposed that, in the near-Earth magnetotail, earthward propagating flux ropes can merge with the Earth's dipole magnetic field and dissipate its magnetic energy. However, the reconnection diffusion region related to this process has not been identified. Here we report the first in situ observation of magnetic reconnection between an earthward propagating flux rope and the closed magnetic field lines connecting to Earth. Magnetospheric Multiscale (MMS) spacecraft crossed a vertical current sheet between the leading edge of the flux rope (negative B(sub z)) and the geomagnetic field (positive B(sub z)). The subion-scale current sheet, super-Alfvénic electron outflow, Hall magnetic and electric field, conversion of magnetic energy to plasma energy (J·E > 0), and magnetic null were observed during the crossing. All the above signatures indicate that MMS detected the reconnection diffusion region. This result is also relevant to other planets with intrinsic magnetosphere.
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- 2018
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43. ODP028 Association Between Serum Steroid Profile and Metabolic Risks in Adults with Classic Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency
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Chang H Ahn, Jaeyoon Shim, Han N Jang, Young A Lee, Man H Choi, and Jung Hee Kim
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Endocrinology, Diabetes and Metabolism - Abstract
Objective In adult patients with congenital adrenal hyperplasia (CAH), long-term health outcomes, including metabolic and cardiovascular health, are a priority for the management of CAH. The enzyme 21-hydroxylase deficiency, which accounts for a majority of CAH, leads to a large perturbation in serum steroid profile under glucocorticoid replacement therapy. Serum levels of several adrenal steroids, including 17-hydroxyprogesterone and androstenedione, are associated with disease severity and sexual dysfunction of CAH patients. However, the association between the serum steroid profiles with metabolic risk in CAH patients is not elucidated yet. We aimed to investigate the serum steroid profile of adult CAH patients using liquid chromatography-mass spectrometry (LC-MS) and their association with metabolic risk. Methods Adult patients with classic CAH due to 21-hydroxylase deficiency were included in this study (36 females and 27 males). A multi-steroid panel composed of 25 steroids and their metabolites was applied to morning serum samples of study subjects using LC-MS. An unsupervised clustering algorithm was applied to the serum steroid panel to discover any unique pattern which divides CAH subjects into clusters. The association between serum steroid profiles and clinical characteristics, including age, sex, body mass index (BMI), subtypes (simple virilizing and salt wasting), glucocorticoid dose, and the presence of hypertension, diabetes mellitus (DM), and metabolic syndrome (MetS) was analyzed. The discriminatory power of each steroid or a combination of steroids was estimated using the area under the curve of receiver operating characteristics (AUROC) for metabolic diseases. Results Fifteen steroids including pregnenolone sulfate, 17α-hydroxyprogesterone, cortisol, cortisone, 21-deoxycortisol, 20α-dihydrocortisol, 20α-dihydrocortisone, α-cortolone, β-cortolone, tetrahydrocortisone, dehydroepiandrosterone sulfate, testosterone, androstenedione, 11β-hydroxytestosterone, and 11β-hydroxyandrostenedione were successfully quantified in all subjects. The median age of subjects was 28 years (interquartile range: 25-35). The prevalence of DM, hypertension, and MetS was 3.2% (2/63), 15.8% (10/63), and 28.6% (18/63), respectively. The subjects were divided into two clusters based on the pattern of serum steroid profile using unsupervised hierarchical clustering. The prevalence of MetS was significantly different between the two clusters (cluster 1, 37.8% [17/45] vs. cluster 2, 5.6% [1/18], P = 0. 011). The prevalence of HTN was numerically higher in cluster 1 than cluster 2 (20. 0% [9/45] vs. 5.6% [1/18], P = 0.257). Other clinical characteristics, including age, sex, BMI, subtypes, and glucocorticoid dose, were not different between the two clusters. Among fifteen steroids, the level of tetrahydrocortisone showed the highest discriminatory power for MetS (AUROC 0.795, 95% confidence interval: 0.675-0.914). The multivariate logistic regression model of all 15 steroids showed AUROC of 0.832 (95% CI: 0.732-0.933) for MetS. Conclusion The serum steroid profiles of CAH patients were significantly associated with the presence of MetS. This suggests that serum steroid signatures can guide the optimal management of adult CAH patients to minimize the risk of MetS. Presentation: No date and time listed
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- 2022
44. Impact of Sex on Outcome After Pulmonary Endarterectomy (PEA) for Chronic Thromboembolic Pulmonary Hypertension (CTEPH)
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Chan, J.C., primary, Man, H., additional, Asghar, U.M., additional, Zhao, Y., additional, Donahoe, L.L., additional, McRae, K., additional, Wu, L., additional, Granton, J., additional, and de Perrot, M., additional
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- 2022
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45. Distinct Endothelial Cell Populations Characterize the Pulmonary Endarterectomy Specimen in Chronic Thromboembolic Pulmonary Hypertension
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Man, H., primary, Zhao, Y.D., additional, Asghar, U., additional, Wu, L., additional, and de Perrot, M., additional
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- 2022
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46. SMAD4 depletion contributes to endocrine resistance by integrating ER and ERBB signaling in HR + HER2− breast cancer
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Kang Li, Dan Shu, Han Li, Ailin Lan, Wenjie Zhang, Zhaofu Tan, Man Huang, Maria Lauda Tomasi, Aishun Jin, Haochen Yu, Meiying Shen, and Shengchun Liu
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Cytology ,QH573-671 - Abstract
Abstract Endocrine resistance poses a significant clinical challenge for patients with hormone receptor-positive and human epithelial growth factor receptor 2-negative (HR + HER2−) breast cancer. Dysregulation of estrogen receptor (ER) and ERBB signaling pathways is implicated in resistance development; however, the integration of these pathways remains unclear. While SMAD4 is known to play diverse roles in tumorigenesis, its involvement in endocrine resistance is poorly understood. Here, we investigate the role of SMAD4 in acquired endocrine resistance in HR + HER2− breast cancer. Genome-wide CRISPR screening identifies SMAD4 as a regulator of 4-hydroxytamoxifen (OHT) sensitivity in T47D cells. Clinical data analysis reveals downregulated SMAD4 expression in breast cancer tissues, correlating with poor prognosis. Following endocrine therapy, SMAD4 expression is further suppressed. Functional studies demonstrate that SMAD4 depletion induces endocrine resistance in vitro and in vivo by enhancing ER and ERBB signaling. Concomitant inhibition of ER and ERBB signaling leads to aberrant autophagy activation. Simultaneous inhibition of ER, ERBB, and autophagy pathways synergistically impacts SMAD4-depleted cells. Our findings unveil a mechanism whereby endocrine therapy-induced SMAD4 downregulation drives acquired resistance by integrating ER and ERBB signaling and suggest a rational treatment strategy for endocrine-resistant HR + HER2− breast cancer patients.
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- 2024
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47. Somatic GNA11/GNAQ variants in a cohort of Chinese children with phakomatosis pigmentovascularis
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Bin Zhang, Rui He, Riga Wu, Zhou Yang, Man Hu, Nan Zhang, Wu Guo, Zigang Xu, and Lin Ma
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Phakomatosis pigmentovascularis ,GNA11 ,GNAQ ,Somatic variant ,Pediatrics ,RJ1-570 - Abstract
ABSTRACT Importance Postzygotic mutations in the GNAQ/GNA11 genes, which encode the G‐protein nucleotide binding protein alpha subunits, have been identified in patients with phakomatosis pigmentovascularis (PPV). However, little is known about the Chinese population. Objective To identify pathogenic mutations in pediatric patients with PPV within the Chinese population. Methods We performed whole‐exome sequencing (WES) using skin lesion tissues from pediatric patients diagnosed with PPV. Additionally, ultradeep‐targeted sequencing was conducted to validate the somatic mutations. A genotype‐phenotype correlation was analyzed by integrating data from previous reports with the findings of the present study. Results Thirteen patients were enrolled, all diagnosed with the cesioflammea type of PPV, except for one patient with an unclassifiable type. We identified somatic GNA11 c.547C>T (p.R183C) variant in seven patients and GNAQ c.548G>A (p.R183Q) in four patients, with low allelic fractions ranging from 2.1% to 8.6% through ultradeep sequencing. Besides, a GNAQ c.548G>A (p.R183Q) variant was detected through targeted sequencing in one of two patients who did not exhibit detectable variants via WES. The genotype‐phenotype correlation analysis, involving 15 patients with a GNA11 variant and 10 with a GNAQ variant, revealed that facial capillary malformation (87% vs. 50%, P = 0.075) and ocular melanocytosis (80% vs. 40%, P = 0.087) appeared to be more frequent in patients with GNA11 mutation compared to those with GNAQ mutations. All four patients diagnosed with cesiomarmorata type or overlapping cesioflammea and cesiomarmorata type PPV carried the GNA11 variant. Interpretation Our study demonstrated that the majority of PPV patients in the Chinese population carried a postzygotic variant of GNAQ/GNA11, thus further confirming the pathogenic role of GNAQ/GNA11 mosaicism in the development of PPV cesioflammea type.
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- 2024
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48. Completion rates and myelosuppression degrees of cancer patients receiving radiotherapy or chemoradiotherapy unchanged regardless of delay duration after Omicron infection
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Zhenyu Zhang, Juan Zhou, Xun Peng, Ping Li, Xue Meng, Man Hu, Miaoqing Zhao, Qinghai Lin, and Kun Ru
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Omicron ,Cancer patients ,Radiotherapy ,Chemoradiotherapy ,Completion rates ,Myelosuppression ,Medicine ,Science - Abstract
Abstract This study aimed to investigate impacts of Omicron infection on cancer patients in China. A retrospective study was conducted, including 347 cancer patients undergoing radiotherapy or chemoradiotherapy between July 2022 and March 2023. Three groups involved: 108 patients without SARS-CoV-2 infection (non-COVID-19 group), 102 patients beginning treatment 10 days after first SARS-CoV-2 infection (≥ 10 days COVID-19 group), and 137 patients beginning treatment less than 10 days after first SARS-CoV-2 infection (
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- 2024
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49. Reducing edge loading and alignment outliers with image-free robotic-assisted unicompartmental knee arthroplasty: a case controlled study
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Wai Hong Lau, Wai Kiu Thomas Liu, Kwong Yuen Chiu, Man Hong Cheung, Amy Cheung, Ping Keung Chan, Vincent Wai Kwan Chan, and Henry Fu
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Unicompartmental knee arthroplasty ,Robotic-assisted surgery ,Component positioning ,Loosening ,Early failure ,Orthopedic surgery ,RD701-811 - Abstract
Abstract Background Survivorship of medial unicompartmental knee arthroplasty (UKA) is technique-dependent. Correct femoral-tibial component positioning associates with improved survivorship. Image-free robotic-assisted unicompartmental knee arthroplasty enables preoperative and intraoperative planning of alignment and assessment of positioning prior to execution. This study aimed to compare the radiological outcomes between robotic-assisted UKA (R-UKA) and conventional UKA (C-UKA). Methods This retrospective case control study involved 140 UKA (82 C-UKA and 58 R-UKA) performed at an academic institution between March 2016 to November 2020, with a mean follow-up of 3 years. Postoperative radiographs were evaluated for mechanical axis and femoral-tibial component position. Component position was measured by two methods: (1) femoral-tibial component contact point with reference to four medial-to-lateral quadrants of the tibial tray and (2) femoral-tibial component contact point deviation from the center of the tibial tray as a percentage of the tibial tray width. Baseline demographics and complications were recorded. Results There was a higher mean component deviation in C-UKA compared with R-UKA using method 2 (17.2% vs. 12.8%; P = 0.007), but no difference in proportion of zonal outliers using method 1 (4 outliers in C-UKA, 5.1% vs. 1 outlier in R-UKA, 1.8%; P = 0.403). R-UKA showed no difference in mean mechanical alignment (C-UKA 5° vs. R-UKA 5°; P = 0.250). 2-year survivorship was 99% for C-UKA and 97% for R-UKA. Mean operative time was 18 min longer for R-UKA (P
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- 2024
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50. Cadmium-induced annulus fibrosus cell senescence contributes to intervertebral disc degeneration via the JNK/p53 signaling pathway
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Xin Liu, Wenjie Zhao, Man Hu, Yu Zhang, Jingcheng Wang, and Liang Zhang
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annulus fibrosus ,cadmium ,c-jun n-terminal kinase ,intervertebral disc-degeneration ,senescence ,tumor suppressor protein- p53 ,Medicine - Abstract
Objective(s): Investigating the impact of cadmium (Cd) on annulus fibrosus (AF) cells and its potential mechanism was the purpose of the current study.Materials and Methods: Cd was cultivated in different concentrations (0, 1, 5, 10, and 20 μM) on AF cells and the potential effects of the metal were assessed. Using the CCK-8 method, cell viability and proliferation were identified. Using transcriptome analysis, the annulus fibrosus cells were sequenced both with and without cadmium chloride. The EdU method was used to determine the rate of cell proliferation; senescence-associated β-galactosidase (SA-β-Gal) staining was used to determine the number of positive cells; and western blot, RT-PCR, and immunofluorescence were used to determine the protein and mRNA expression of senescence-associated proteins (p16, p21, and p53) and c-Jun N-terminal kinase (JNK).Results: According to the findings, Cd has the ability to increase the production of senescence-associated genes (p16 and p21) and senescence-associated secreted phenotype (SASP), which includes IL-1β and IL-6. Through the JNK/p53 signal pathway, Cd exposure simultaneously accelerated AF cell senescence and promoted SASP. Following JNK inhibitor (SP600125) treatment, the expression of p53, JNK, and senescence-associated indices were all down-regulated.Conclusion: By activating the JNK/p53 signaling pathway, Cd can induce oxidative stress damage and AF cell senescence. These findings could provide a new approach for treating and preventing intervertebral disc degeneration (IVDD) caused by Cd exposure.
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- 2024
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