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7. A scalable serology solution for profiling humoral immune responses to SARS‐CoV‐2 infection and vaccination

Author

Colwill, Karen, primary, Galipeau, Yannick, additional, Stuible, Matthew, additional, Gervais, Christian, additional, Arnold, Corey, additional, Rathod, Bhavisha, additional, Abe, Kento T, additional, Wang, Jenny H, additional, Pasculescu, Adrian, additional, Maltseva, Mariam, additional, Rocheleau, Lynda, additional, Pelchat, Martin, additional, Fazel‐Zarandi, Mahya, additional, Iskilova, Mariam, additional, Barrios‐Rodiles, Miriam, additional, Bennett, Linda, additional, Yau, Kevin, additional, Cholette, François, additional, Mesa, Christine, additional, Li, Angel X, additional, Paterson, Aimee, additional, Hladunewich, Michelle A, additional, Goodwin, Pamela J, additional, Wrana, Jeffrey L, additional, Drews, Steven J, additional, Mubareka, Samira, additional, McGeer, Allison J, additional, Kim, John, additional, Langlois, Marc‐André, additional, Gingras, Anne‐Claude, additional, and Durocher, Yves, additional

Published
2022
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8. Characterization of Systemic and Mucosal Humoral Immune Responses to an Adjuvanted Intranasal SARS-CoV-2 Protein Subunit Vaccine Candidate in Mice.

Author

Maltseva, Mariam, Galipeau, Yannick, Renner, Tyler M., Deschatelets, Lise, Durocher, Yves, Akache, Bassel, and Langlois, Marc-André

Subjects
SARS-CoV-2 Delta variant, SARS-CoV-2 Omicron variant, BREAKTHROUGH infections, SARS-CoV-2, CHOLERA toxin
Abstract

Continuous viral evolution of SARS-CoV-2 has resulted in variants capable of immune evasion, vaccine breakthrough infections and increased transmissibility. New vaccines that invoke mucosal immunity may provide a solution to reducing virus transmission. Here, we evaluated the immunogenicity of intranasally administered subunit protein vaccines composed of a stabilized SARS-CoV-2 spike trimer or the receptor binding domain (RBD) adjuvanted with either cholera toxin (CT) or an archaeal lipid mucosal adjuvant (AMVAD). We show robust induction of immunoglobulin (Ig) G and IgA responses in plasma, nasal wash and bronchoalveolar lavage in mice only when adjuvant is used in the vaccine formulation. While the AMVAD adjuvant was more effective at inducing systemic antibodies against the RBD antigen than CT, CT was generally more effective at inducing overall higher IgA and IgG titers against the spike antigen in both systemic and mucosal compartments. Furthermore, vaccination with adjuvanted spike led to superior mucosal IgA responses than with the RBD antigen and produced broadly targeting neutralizing plasma antibodies against ancestral, Delta and Omicron variants in vitro; whereas adjuvanted RBD elicited a narrower antibody response with neutralizing activity only against ancestral and Delta variants. Our study demonstrates that intranasal administration of an adjuvanted protein subunit vaccine in immunologically naïve mice induced both systemic and mucosal neutralizing antibody responses that were most effective at neutralizing SARS-CoV-2 variants when the trimeric spike was used as an antigen compared to RBD. [ABSTRACT FROM AUTHOR]

Published
2023
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10. A scalable serology solution for profiling humoral immune responses to SARS-CoV-2 infection and vaccination

Author

Colwill, Karen, primary, Galipeau, Yannick, additional, Stuible, Matthew, additional, Gervais, Christian, additional, Arnold, Corey, additional, Rathod, Bhavisha, additional, Abe, Kento T, additional, Wang, Jenny H, additional, Pasculescu, Adrian, additional, Maltseva, Mariam, additional, Rocheleau, Lynda, additional, Pelchat, Martin, additional, Fazel-Zarandi, Mahya, additional, Iskilova, Mariam, additional, Barrios-Rodiles, Miriam, additional, Bennett, Linda, additional, Yau, Kevin, additional, Cholette, François, additional, Mesa, Christine, additional, Li, Angel X, additional, Paterson, Aimee, additional, Hladunewich, Michelle A, additional, Goodwin, Pamela J, additional, Wrana, Jeffrey L, additional, Drews, Steven J, additional, Mubareka, Samira, additional, McGeer, Allison J, additional, Kim, John, additional, Langlois, Marc-André, additional, Gingras, Anne-Claude, additional, and Durocher, Yves, additional

Published
2021
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12. Analysis of the Antigenic Composition and Differential Incorporation of Host Membrane Proteins into Murine Leukemia Virus by Flow Virometry

Author

Maltseva, Mariam

Subjects
Flow Virometry, viruses, Virology, Retroviruses, HIV, MLV, Flow Cytometry
Abstract

Traditionally, viral particles have been primarily analyzed as a whole population according to their biochemical, genetic, and biophysical properties. Here, we describe single particle phenotypic analysis using surface markers found on Murine Leukemia Virus (MLV) by flow virometry. We used this technology to show differential incorporation of host surface markers between wild type MLV and glycosylated Gag (glycogag) deficient MLV. Moreover, we analyzed differential uptake efficiency of host proteins between two cell lines and primary lymphocytes. We hypothesize that the phenotypic profiling and quantification of antigens on the surface of individual viral particles will provide crucial information on the identity of the infected parental cells. Furthermore, we demonstrate that the MLV accessory protein glycogag is associated with the upregulation of surface antigen incorporation during assembly and release. Aside from possible evolutionary implications of glycogag, we demonstrate presence and varying antigenic composition on the surface of MLV viral particles reflective of the cell phenotype that they were released from.

Published
2020
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