48 results on '"Malenčić, Djordje"'
Search Results
2. EFFECT OF SALINITY STRESS ON ANTIOXIDANT ACTIVITY AND GRAIN YIELD OF DIFFERENT WHEAT GENOTYPES
- Author
-
MATKOVİĆ STOJŠİN, Mirela, primary, PETROVİĆ, Sofija, additional, DİMİTRİJEVİĆ, Miodrag, additional, ŠUĆUR, Jovana, additional, MALENČİĆ, Djordje, additional, ZEČEVİĆ, Veselinka, additional, BANJAC, Borislav, additional, and KNEŽEVİĆ, Desimir, additional
- Published
- 2022
- Full Text
- View/download PDF
3. Posležetvene promene u sadržaju šećera i organskih kiselina u sortama trešnje pod uticajem Monilinia laxa
- Author
-
Kiprovski, Biljana, Kiprovski, Biljana, Malenčić, Djordje, Borković, Boško, Kolarov, Radenka, Veberič, Robert, Mikulic-Petkovsek, Maja, Kiprovski, Biljana, Kiprovski, Biljana, Malenčić, Djordje, Borković, Boško, Kolarov, Radenka, Veberič, Robert, and Mikulic-Petkovsek, Maja
- Abstract
Cilj rada je bio evaluacija uticaja posležetvene, veštačke inokulacije fitopatogenom gljivom Monilinia laxa na sadržaj šećera i organskih kiselina u plodovima devet sorti trešnje (Prunus avium)., The aim of the study was to evaluate impact of postharvest, artificial inoculation with Monilinia laxa pathogenic fungus on sugars and organic acids contents in fruits of nine sweet cherry (Prunus avium) cultivars: ‘Burlat’, ‘Priusadebnaja’, ‘Asenova rana’, ‘Lionska’, ‘Sue’, ‘Junska rana’, ‘Merchant’, ‘Summit’ and ‘3/VAL'. M. laxa represents one of the major pre- and postharvest pathogens of fresh fruits, causing significant economic losses. Due to different possibility of disease incidence in tested cultivars, artificial inoculation would explain how this pathogen affects quality of tested fruits. Determination of individual sugars and organic acids was performed by HPLC methods. For sugars, mobile phase was twice distilled water (0.6 mL/min); Rezex RCMmonosaccharide Ca+ (2%) column (300mmx7.8 mm, Phenomenex) operated at 65 °C, 30 min the total run time. Organic acids were analyzed as follows: 4 mM sulphuric acid in twice distilled water 30 min, Rezex ROA-organic acid H+ (8%) column operated at 65 °C, UV detector 210 nm. Contents of sugars in this study were (in g/kg dry weight): glucose 205-439 and 268-443, fructose 175-398.9 and 208.6-365.8 and sucrose 20-47.6 and 19.2-38.6, in healthy and infected fruits, respectively. Organic acids detected in sweet cherry cultivars were citric, malic, quinic, shikimic and fumaric acid, and their content varied depending on cultivar and treatment. Only ‘Burlat’ accumulated tartaric acid after the infection. ‘Junska rana’ and ‘Merchant’ had significantly decreased organic acids content after the infection, while ‘Sue’ and 3/VAL reacted to the infection by accumulation, especially of fumaric acid.
- Published
- 2022
4. Sugars and organic acids content in unripe and ripe fruits of Sambucus nigra
- Author
-
Kiprovski, Biljana, Kiprovski, Biljana, Malenčić, Djordje, Ognjanov, Vladislav, Veberič, Robert, Hudina, Metka, Mikulič-Petkovšek, Maja, Kiprovski, Biljana, Kiprovski, Biljana, Malenčić, Djordje, Ognjanov, Vladislav, Veberič, Robert, Hudina, Metka, and Mikulič-Petkovšek, Maja
- Abstract
Elderberry (Sambucus nigra L.) is a common plant used for its medicinal and nutritional properties, as for the beverages, jams, liqueurs, flavourings etc. Plant material examined were leaves and fruits of wild edible, and cultivated elderberry plants (’Haschberg’ and ’Ljubostinja’ selections). Determination of organic acids and sugars was performed using high-performance liquid chromatography. Organic acids found in the tested fruit samples were citric, tartaric, malic, quinic, shikimic and fumaric acids, out of which quinic acid content was the highest in unripe, while citric acid content was the highest in ripe fruits. Wild plants had higher contents of organic acids in unripe fruits (16.7 g 100 g-1dw) than cultivated (’Haschberg’ 6.6, and ’Ljubostinja’ 6.3 g 100 g-1dw). ’Ljubostinja’ selection had significantly higher organic acids content (10.2 g 100 g-1dw) in ripe fruits compared to wild specimen and ’Haschberg’ plants which had similar organic acids content (7.0 and 7.4 g 100 g-1dw). The major organic acid in ripe elderberry fruits was citric acid. When it comes to sugars content the presence of sucrose, glucose, fructose and sorbitol was established. Wild specimen had significantly higher sugars content (2.2 in unripe, and 8.4 g 100 g-1dw in ripe fruits).
- Published
- 2022
5. Total and individual phenolics in wild and cultivated elderberry during development
- Author
-
Kiprovski, Biljana, Kiprovski, Biljana, Malenčić, Djordje, Ljubojević, Mirjana, Ognjanov, Vladislav, Veberič, Robert, Mikulič-Petkovšek, Maja, Kiprovski, Biljana, Kiprovski, Biljana, Malenčić, Djordje, Ljubojević, Mirjana, Ognjanov, Vladislav, Veberič, Robert, and Mikulič-Petkovšek, Maja
- Abstract
Compounds which add to bioactivity of elderberry (Sambucus nigra L.) are mostly phenolic compounds. One wild edible and two cultivated genotypes (’Haschberg’ and ’Ljubostinja’ selections) have been chosen. Leaves and fruits were collected in four stages (April-September). Total phenolics (TP) content was determined from MeOH extracts by Folin-Ciocalteau method while determination of individual compounds was performed using HPLC-DAD-ESI-MSn analysis with a DAD at 280, 350 and 530 nm. TP content in leaves varied through developmental stages, being higher in II and III stage. Leaves and fruits (both unripe and ripe) of ’Ljubostinja’ plants had higher content of TP compared to wild ripe fruits (1.5-fold). Leaves had caffeic acid derivatives (0.3-0.7% in wild, 0.2-0.3% in ’Haschberg’ and 0.4-0.6% in ’Ljubostinja’ in dry weigh) and caffeoylquinic acid derivatives, while fruits had more caffeoylquinic acid derivatives, particularly unripe fruits. The major phenolic acid present in leaves, especially during the II stage of development, was chlorogenic acid (approx. 4% dw) and the same was recorded in fruits (0.03% dw in unripe fruits of wild plants). Content of phenolic acids decreased during ripening of elderberry fruits and the similar was detected in the leaves.
- Published
- 2022
6. Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum
- Author
-
Malenčić Djordje, Cvejić Jelena, Tepavčević Vesna, Bursać Mira, Kiprovski Biljana, and Rajković Miloš
- Subjects
glycine max ,isoflavone phytoalexins ,l-phenylalanine ammonia-lyase ,sclerotinia sclerotiorum ,Biology (General) ,QH301-705.5 - Published
- 2013
- Full Text
- View/download PDF
7. Anatomical characteristics and antioxidant ability of Centaurea sadleriana reveals an adaptation towards drought tolerance
- Author
-
Luković Jadranka, Malenčić Djordje, Zorić Lana, Kodranov Miroslava, Karanović Dunja, Kiprovski Biljana, and Boža Pal
- Subjects
leaf anatomy ,peduncle anatomy ,drought tolerance ,antioxidant activity ,asteraceae ,centaurea ,trichomes ,Biology (General) ,QH301-705.5 - Published
- 2013
- Full Text
- View/download PDF
8. Cholic acid changes defense response to oxidative stress in soybean induced by Aspergillus niger
- Author
-
Malenčić Djordje, Kevrešan Slavko, Popović Milan, Štajner Dubravka, Popović Boris, Kiprovski Biljana, and Djurić Simonida
- Subjects
aspergillus niger ,cholic acids ,lipid peroxidation ,oxidative stress ,reactive oxygen species ,Biology (General) ,QH301-705.5 - Published
- 2012
- Full Text
- View/download PDF
9. Anatomical characteristics and antioxidant properties of Euphorbia nicaeensis ssp. glareosa
- Author
-
Luković Jadranka, Malenčić Djordje, Zorić Lana, Kiprovski Biljana, Merkulov Ljiljana, and Boža Pal
- Subjects
anatomy ,antioxidant activity ,euphorbia ,e. nicaeensis ,Biology (General) ,QH301-705.5 - Published
- 2009
- Full Text
- View/download PDF
10. Effects of linuron and dimethenamid on antioxidant systems in weeds associated with soybean
- Author
-
Malenčić Djordje, Miladinović Jegor, and Popović Milan
- Subjects
lipid peroxidation ,oxidative stress ,reactive oxygen species ,reduced glutathione ,superoxide dismutase ,total phenols ,Biology (General) ,QH301-705.5 - Published
- 2008
- Full Text
- View/download PDF
11. Correlation between lipid peroxidation and phenolics content in leaves and roots of sugar beet infected with Rhizoctonia solani
- Author
-
Kiprovski, Biljana, Malenčić, Djordje, Popović, Milan, Stojšin, Vera, Budakov, Dragana, Ćurčić, Živko, and Danojević, Dario
- Published
- 2014
- Full Text
- View/download PDF
12. Effect of extraction solvents on the antioxidant activity of industrial hemp extracts
- Author
-
Prvulović, Dejan, Prvulović, Dejan, Gvozdenac, Sonja, Peić Tukuljac, Marijana, Malenčić, Djordje, Kiprovski, Biljana, Sikora, Vladimir, Latković, Dragana, Prvulović, Dejan, Prvulović, Dejan, Gvozdenac, Sonja, Peić Tukuljac, Marijana, Malenčić, Djordje, Kiprovski, Biljana, Sikora, Vladimir, and Latković, Dragana
- Abstract
The objective of this study was to determine the content of phenolic compounds (total phenolics, total tannins and total flavonoids) and antioxidant capacity by six different assays in industrial hemp (‘Helena’ variety) extracted by four different extraction solvents: 70% methanol, 70% ethanol, 70% acetone and water. Out of the four solvent mixtures evaluated in the current study for the extraction of phenolic compounds, the use of 70% acetone yielded to the highest total contents of phenolics and exhibited the highest antioxidant activity in all performed assays.
- Published
- 2019
13. Changes in polyphenols content in soybean and Datura stramonium after treatment with herbicides and Delfan Plus
- Author
-
Malenčić, Djordje, Malenčić, Djordje, Kiprovski, Biljana, Prvulović, Dejan, Rajković, Miloš, Malidža, Goran, Malenčić, Djordje, Malenčić, Djordje, Kiprovski, Biljana, Prvulović, Dejan, Rajković, Miloš, and Malidža, Goran
- Abstract
The most common herbicide combination for weed prevention in soybean is Pulsar 40 and Harmony 75WG, but when applied during hot days these herbicides may induce oxidative stress in plants. Delfan Plus® is a biostimulator with high amino acid content which can be used in combination with herbicides. Manufacturer advertises it as a supplement for faster protein synthesis and thus, overcoming stress conditions caused by high and low temperatures, drought, herbicides etc. In order to investigate the ability of Delfan Plus® to mitigate the effect of herbicides on soybean plants (and its weed species Datura stramonium L.), we analyzed the accumulation of polyphenolic compounds (total polyphenols, tannins, flavonoids and proanthocyanidins), as well as phytotoxicity and grain yield. In addition, we determined the antioxidant capacity of plant extracts in DPPH and NBT-tests. Soybean cultivar Sava was treated with Pulsar 40 + Harmony 75WG (1 L ha-1 + 8 g ha-1 and 2 L ha-1 + 16 g ha-1, respectively), with or without Delfan Plus® (1 L ha-1 and 2 L ha-1, respectively). Biochemical parameters analyzed showed that Delfan plus® did not decrease the level of stress in plants. Most parameters increased their values 7 days after the treatment, compared to day 1, which was expected. Specimens of D. stramonium were especially stressed after herbicide treatment. Although soybean yield was somewhat higher after the treatment with normal dose of herbicides and Delfan plus® (3.28 t ha-1), there were no statistical differences between the treatments. Hence, the question of the application of this biostimulator in agronomic practice may be raised.
- Published
- 2015
14. Rutin content in seeds of European buckwheat (Fagopyrum esculentum) cultivars
- Author
-
Biljana Kiprovski, Popović, Vera, Mikulic-Petkovsek, Maja, and Malenčić, Djordje
- Subjects
Fagopyrum esculentum Moench ,quercetin-3-rutinoside ,phenolics ,rutin ,flavonoids ,HPLC-DAD ,Buckwheat ,polyphenols - Abstract
Buckwheat (Fagopyrum esculentum Moench) is an important functional food and rich source of vitamins, essential amino acids and phenolics, responsible for many of the health benefits and antioxidant properties. This pseudocereal contains quercetin-3-rutinoside or rutin as a major phenol in seeds, approx. 90.4% of total flavonols content. Rutin is an antioxidant that has many pharmacologically useful properties, such as anti-inflammatory, anticarcinogenic, antithrombotic, cytoprotective and vasoprotective effects. The aim of this study was to investigate content of rutin in seeds of cultivars from Western, Central Europe and Balkans: Serbia (´Novosadska´), Slovenia (´Darja´, ´Prekmurska´ and ´Cebelica´), Bosnia and Herzegovina (´Bosna 1´ and ´Bosna 2´), Montenegro (´Godijevo´ and ´Lokve´), Austria (´Bamby´), Czech Republic (´Ceska´), France (´La Harpe´), Slovakia (´Spacinska 1´) grown in the Balkan area and to compare them with indigenous Balkan buckwheat cultivars. Rutin content was determined using HPLC-DAD analysis, on a Thermo Finnigan Surveyor HPLC system with a diode array detector at 350 nm. ´Bosna 1´ (114.6 mg 100 g-1 dry weight) and ´Bosna 2´ (151.4 mg 100 g-1 dry weight) were highlighted with the greatest rutin content, 13.6-34.7 and 18.0-45.9 times higher than in other cultivars, respectively. Beside these two cultivars, a scale made according to rutin content in buckwheat seeds organise investigated cultivars in the following order: ´Novosadska´ > ´La Harpe´ >´ Godijevo´ >´ Darja´ > ´Cebelica´ > ´Bamby´ > ´Ceska´ > ´Spacinska 1´ > ´Prekmurska´ > ´Lokve´. These results suggest that indigenous buckwheat cultivars contain important dietary antioxidants and could be of great interest for buckwheat breeders and an important source of functional food due to significant differences in their contents among cultivars.
- Published
- 2017
15. Rutin content in seeds of European buckwheat (Fagopyrum esculentum) cultivars
- Author
-
Kiprovski, Biljana, Kiprovski, Biljana, Popović, Vera, Mikulic-Petkovsek, Maja, Malenčić, Djordje, Kiprovski, Biljana, Kiprovski, Biljana, Popović, Vera, Mikulic-Petkovsek, Maja, and Malenčić, Djordje
- Abstract
Buckwheat (Fagopyrum esculentum Moench) is an important functional food and rich source of vitamins, essential amino acids and phenolics, responsible for many of the health benefits and antioxidant properties. This pseudocereal contains quercetin-3-rutinoside or rutin as a major phenol in seeds, approx. 90.4% of total flavonols content. Rutin is an antioxidant that has many pharmacologically useful properties, such as anti-inflammatory, anticarcinogenic, antithrombotic, cytoprotective and vasoprotective effects. The aim of this study was to investigate content of rutin in seeds of cultivars from Western, Central Europe and Balkans: Serbia (´Novosadska´), Slovenia (´Darja´, ´Prekmurska´ and ´Cebelica´), Bosnia and Herzegovina (´Bosna 1´ and ´Bosna 2´), Montenegro (´Godijevo´ and ´Lokve´), Austria (´Bamby´), Czech Republic (´Ceska´), France (´La Harpe´), Slovakia (´Spacinska 1´) grown in the Balkan area and to compare them with indigenous Balkan buckwheat cultivars. Rutin content was determined using HPLC-DAD analysis, on a Thermo Finnigan Surveyor HPLC system with a diode array detector at 350 nm. ´Bosna 1´ (114.6 mg 100 g-1 dry weight) and ´Bosna 2´ (151.4 mg 100 g-1 dry weight) were highlighted with the greatest rutin content, 13.6-34.7 and 18.0-45.9 times higher than in other cultivars, respectively. Beside these two cultivars, a scale made according to rutin content in buckwheat seeds organise investigated cultivars in the following order: ´Novosadska´ > ´La Harpe´ >´ Godijevo´ >´ Darja´ > ´Cebelica´ > ´Bamby´ > ´Ceska´ > ´Spacinska 1´ > ´Prekmurska´ > ´Lokve´. These results suggest that indigenous buckwheat cultivars contain important dietary antioxidants and could be of great interest for buckwheat breeders and an important source of functional food due to significant differences in their contents among cultivars.
- Published
- 2017
16. Correlation between phenolic content and antioxidant activity in fruits of Prunus avium infected with Monilia laxa
- Author
-
Borković, Boško, Malenčić, Djordje, Prvulović, Dejan, Biljana Kiprovski, and Barać, Goran
- Subjects
antioxidants ,food and beverages ,Monilinia laxa ,antioxidant capacity ,Prunus avium ,polyphenols - Abstract
Accumulation of phenolic polymers reinforce plant cell walls, limiting fungal penetration. Some of these compounds, such as isochlorogenic acid, are particularly active in limiting fungal extension. Two main features of phenolic compounds are that they are produced in greater quantities by resistant plant genotypes, and that they have the ability to limit plant pathogens growth. Thus, they could be considered as potential markers of resistance to Monilinia laxa in sweet cherrie breeding programs. We investigated the content of the total phenols (TP), total tannins (TT) and anthocyanins (A) in the fruits of ten sweet cherrie cultivars, before and after the infection with M. laxa, under the field conditions. In addition to this, we determined the total antioxidant capacity of the fruit extracts using the DPPH-free radical scavenging assay and FRAP method. The correlations between phenolic classes and antioxidant activity have been calculated, as well. Results showed that the TP content ranged from 3.71 to 7.52 mg gallic acid equivalent (GAE) g-1 dry weight depending on the genotype and the infection. TT content was lower and it ranged from 0.52 to 2.64 mg GAE g-1 dry weight. Anthocyanins content ranged from 0.29 to 2.71 mg cyanidin-3-glucoside g-1 dry weight. DPPH values ranged from 38.47 to 63.50 % of neutralised radicals, while FRAPvalues were 14.73-33.69 mg trolox g-1 fresh weight. No positive correlation was found for antioxidant tests and phenolic compounds investigated. Still, TP and TT were higher in infected fruits of five specimen (Junska rana, Summit, Mershant, Priusodebnaja and Burlat), and A levels were higher in the same cultivars except for the cv. Summit. These cultivars accumulate defensive compounds under the pathogen induced stress and may be considered as more tolerant genotypes with more desired traits for production, selection and breeding programmes.
- Published
- 2016
17. Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress
- Author
-
Dorić, Dušica, Malenčić, Djordje, Biljana Kiprovski, Barać, Goran, Ljubojević, Mirjana, and Ognjanov, Vladislav
- Subjects
softwood cuttings ,abiotic stress ,superoxide-dismutase ,auxins ,Prunus sp - Abstract
Oxidative metabolism of normal cells and different stress situations generate highly reactive oxygen species (ROS). The ROS have been implicated in a number of physiological disorders in plants. Such factors as certain mechanical injuries are known to induce ROS formation in most aerobic organisms. Antioxidant defence systems have co-evolved with aerobic metabolism to counteract oxidative damage from ROS. This includes antioxidant enzymes such as superoxide-dismutase (SOD; EC 1.15.1.1). SOD is of the greatest importance since it catalyzes the dismutation of the superoxide radical (O2.-) to O2 and H2O. Thus, the aim of this study was to measure the intensity of SOD in the leaves of six cherry rootstock selections in order to investigate the effect of exogenously applied auxins on the mechanical injury induced oxidative stress during the process of rooting of softwood cuttings. The experiment was carried out in the plastic house under a fogging system with 95-99% average relative humidity. The 15-20 cm long terminal cuttings were treated with the mix of auxins (IBA, 0.5% and α-NAA, 0.8%) powder prior to insertion into the white sphagnum and perlite substrate mixture. Leaves of six promising rootstock selections of Prunus cerasus L., P. mahaleb L. and P. fruticosa Pall., and one standard rootstock PHL-A were sampled on the 1st, 3rd and 7th day after inserting softwood cuttings into the rooting substrate. The SOD activity was measured by monitoring the inhibition of nitroblue-tetrazolium (NBT) reduction at 560 nm. Results showed that generation of ROS occurred in all investigated genotypes, but they greatly differed in susceptibility towards oxidative stress induces by mechanical injury. Application of auxins on standard vegetative rootstock PHL-A, as well as P. mahaleb selection M6 showed positive effect on lowering SOD activity after the 3rd and 7th collecting day compared to control. On the other hand, selections of P. cerasus OV22 and P. fruticosa SV2 expressed high SOD activity during all collecting days which was from 3.6-54.9% higher compared to control. Enhanced SOD activity in leaves of softwood cuttings indicates induction of the enzymatic antioxidant system in both auxin treated and untreated cutting. According to these results SOD activity could be used as one of the biochemical parameters in further rootstock selection for sweet and sour cherries.
- Published
- 2016
18. Lipid peroxidation intensity in soybean and maize plants inoculated with PGPR
- Author
-
Biljana Kiprovski, Koleška, Ivana, Malenčić, Djordje, Rajković, Miloš, Djuric, Simonida, Sikora, Vladimir, and Đurić, Gordana
- Subjects
plant growth-promoting rhizobacteria ,PGPR ,PGPB ,fungi ,food and beverages ,oxidative stress ,lipid peroxidation ,soybean ,maize - Abstract
The purpose of this work was to define the reaction of soybean and maize plants to inoculation with plant growth-promoting rhizobacteria (PGPR) [isolates of Azotobacter (AB), Streptomyces (S) and mixture of these (MIX)], by investigating lipid peroxidation intensity. Lipid peroxidation (LP) represents a valuable biomarker of cell degradation and oxidative stress secondary effects. It is analyzed as a response of plants exposed to various (a)biotic factors and in this work it is expressed as nmol malondialdehyde (MDA) equivalents in fresh leaves and roots of investigated plants. Seeds of soybean (cultivar Bečejka) and maize (hybrid NS 640) were inoculated with aqueous inoculums of tested PGPRs and grown under field conditions, without fertilization. Plants were harvested for biochemical analyses at three specific stages of development: 21-day-old seedlings, full bloom, seed beginning stage and, at the end of the experiments, yield was recorded. Inoculated plants had similar values of LP intensity as plants from control (35.1-98.9 nmol MDA g-1 fresh weight). There were no significant differences in LP intensity between control and treatments within the sampling stage, however the amount of MDA accumulated during the vegetation period (up to 60%), possibly because of the developmental processes in soybean and maize. As for seed yield (t ha-1), both investigated species had 5-7% higher yield when inoculated with MIX inoculum, which highlighted the coupled inoculation as possible potent biofertilizer in soybean and maize organic production.
- Published
- 2016
19. Microcystin accumulation and potential effects on antioxidant capacity of leaves and fruits of Capsicum annuum
- Author
-
Drobac, Damjana, primary, Tokodi, Nada, additional, Kiprovski, Biljana, additional, Malenčić, Djordje, additional, Važić, Tamara, additional, Nybom, Sonja, additional, Meriluoto, Jussi, additional, and Svirčev, Zorica, additional
- Published
- 2017
- Full Text
- View/download PDF
20. Activity of nitrogen assimilation enzymes in soybean seedlings infected with hemibiotrophic fungi
- Author
-
Biljana Kiprovski, Malenčić, Djordje, Uzelac, Branka, and Šiler, Branislav
- Subjects
biotic stress ,fungi ,seedlings ,food and beverages ,soybean ,hemibiotrophic fungi ,nitrogen metabolism - Abstract
The purpose of this research was to compare how soybean seedlings (Glycine max L., cultivar Bečejka) cope with different nutrition acquisition strategies of hemibiotrophic fungi: Rhizoctonia solani Kühn and Sclerotinia sclerotiorum (Lib.) de Bary. Severe changes at morphological and histological level after inoculation with both fungi were accompanied by significant changes in nitrogen assimilation enzymes activities in leaves and roots of 21-day-old soybean plants. Infected seedlings had decreased nitrate reductase (NR) (2-fold the amount of control, on average) and glutamate synthase (GS) activity (40-60%), except in leaves infected with S. sclerotiorum. Glutamate dehydrogenase (GDH) activity increased 46-75% after the pathogen infection, being highest during R. solani infection. High GDH values in infected organs (0.26-0.47 μmol NADH mg-1 protein) point to enhanced nitrogen remobilization process from infected tissue, possibly to restrict available nutrients to pathogens, among other things beneficial to plant. Differences in GS and GDH activities in the same organs depending on pathogen infection showed that plants cope differently with these fungi at this stage of development, or that time of switching from bio- to necrotrophic lifestyle differs between investigated pathogens. Due to adaptable lifestyle of hemibiotrophic fungi, mechanistic details that allow pathogen to control host metabolic pathways are unknown, and for this reason the understanding of plant nutrient acquisition could be of great importance in the development of novel disease control strategies.
- Published
- 2015
21. Fitohemijski sastav, lekoviti potencijal i senzorne karakteristike sorti vrsta Brassica oleracea L. i Brassica rapa L. (Brassicaceae) iz organskog i konvencionalnog sistema proizvodnje
- Author
-
Mimica-Dukić, Neda, Orčić, Dejan, Malenčić, Djordje, Beara, Ivana, Četojević-Simin, Dragana, Bošković, Svetlana, Mimica-Dukić, Neda, Orčić, Dejan, Malenčić, Djordje, Beara, Ivana, Četojević-Simin, Dragana, and Bošković, Svetlana
- Abstract
U ovom radu su ispitani hemijski sastav i biološka aktivnost vrsta Brassica oleracea i Brassica rapa poreklom iz organskog i konvencionalnog sistema proizvodnje. Ispitivanja hemijskog sastava su obuhvatila određivanje volatilnih komponenata primenom headspace GC-MS tehnike, kvalitativnu analizu sokova ispitivanih vrsta pomoću LC-DAD-MS/MS tehnike, kvantifikaciju odabranih fenolnih jedinjenja pomoću LC-MS/MS tehnike i spektrofotometrijsko određivanje sadržaja ukupnih glukozinolata, fenola i flavonoida i sadržaja ukupnih monomernih antocijana. Biološka aktivnost povrća je ispitana određivanjem antioksidantnog potencijal, antimikrobne i antitumorske aktivnosti sokova. Dobijeni rezultati pokazuju da su glavne volatilne komponente ispitivanih vrsta degradacioni proizvodi masnih kiselina i organosumpornih jedinjenja od kojih neke od identifikovanih komponenata predstavljaju ključne mirisne komponente. Na osnovu njihove distribucije nije bilo moguće utvrditi uticaj sistema proizvodnje na miris i ukus ispitivanih vrsta. Pomoću LC-DAD-MS/MS i LC-MS/MS tehnike je utvrđeno prisustvo primarnih metabolita, slobodnih hidroksibenzoevih i hidroksicimetnih kiselina, derivate hinske kiseline sa hidroksicimetnim kiselinama, glikozida flavonola, biflavonoida, i kumarina u sokovima spitivanih vrsta. Pored toga u svim sokovima subili prisutni kondenzacioni proizvodi degradacionih proizvoda indolnih glukozinolata sa askorbinskom kiselinom- askorbigen i metoksiaskorbigen. U sokovima crvenog kupusa su bili prisutni antocijani. Najveći sadržaj ukupnih glukozinolata je određen u brokoliju, dok je crveni kupus sadržao najveći sadržaj ukupnih fenola ukupnih flavonoida kao i hinske kiseline. Crveni kupus je bio varijetet koji je ispoljio najviši antioksidantni kapacitet u svim primenjenim testovima, brokoli i karfiol su ispoljili najjaču antimikrobnu aktivnost, dok je kelj bio varijetet sa najjačim antitumorskim delovanjem. Na osnovu analize hemijskog sastava i biološke aktivnosti vrsta B., Chemical composition and biological activity of Brassica oleracea and B. rapa species were investigated in the present work. Investigation of chemical composition encompassed determination of volatile compounds by headspace GC-MS technique, qualitative analysis of juices of investigated vegetables by LC-DAD-MS/MS technique, quantification of selected phenolic compounds by LC-MS/MS technique and spectrophotometric determination of total content of glucosinolates, phenolic compounds and flavonoids and total content of monomeric anthocyanes. Biological activity of vegetables was investigated by determination of antioxidant capacity, antimicrobial and antitumorigenic effect of juices. Obtained results showed that main volatile compounds were degradation products of fatty acids and organo-sulphur compounds among which some of the identified components were the key aroma compounds. Based on their distribution it was not possible to evaluate influence of cropping system on aroma and taste of investigated species. By LC-DAD-MS/MS and LC-MS/MS techniques, it was determined the presence of primary metabolites, freehydroxybenzoic and hydroxycinnamic acids, derivatives of quinic acid and hidroxicinnamic acids, flavonol glucosides, biflavnoids and a cumarin in juices of the investigated species. Furthermore, condensation products of degradation products of indole glucosinolates with ascorbic acid- ascorbigen and metoxiascorbigen were present in all juices. Anthocyanes were present in juices of red cabbage. The highest total content of glucosinolates was determined in broccoli, while red cabbage had the highest total phenolic content, total flavonoid content, as well as content of qunic acid. Red cabbage was a variety that showed the highest antioxidant capacity measured by different assays. Broccoli and cauliflower expressed the highest antimicrobial potential, while Savoy cabbage was a variety with the highest antitumor effect. Investigation of chemical composition and b
- Published
- 2016
22. Lipid peroxidation intensity in soybean and maize plants inoculated with PGPR
- Author
-
Kiprovski, Biljana, Kiprovski, Biljana, Koleška, Ivana, Malenčić, Djordje, Rajković, Miloš, Djuric, Simonida, Sikora, Vladimir, Kiprovski, Biljana, Kiprovski, Biljana, Koleška, Ivana, Malenčić, Djordje, Rajković, Miloš, Djuric, Simonida, and Sikora, Vladimir
- Abstract
The purpose of this work was to define the reaction of soybean and maize plants to inoculation with plant growth-promoting rhizobacteria (PGPR) [isolates of Azotobacter (AB), Streptomyces (S) and mixture of these (MIX)], by investigating lipid peroxidation intensity. Lipid peroxidation (LP) represents a valuable biomarker of cell degradation and oxidative stress secondary effects. It is analyzed as a response of plants exposed to various (a)biotic factors and in this work it is expressed as nmol malondialdehyde (MDA) equivalents in fresh leaves and roots of investigated plants. Seeds of soybean (cultivar Bečejka) and maize (hybrid NS 640) were inoculated with aqueous inoculums of tested PGPRs and grown under field conditions, without fertilization. Plants were harvested for biochemical analyses at three specific stages of development: 21-day-old seedlings, full bloom, seed beginning stage and, at the end of the experiments, yield was recorded. Inoculated plants had similar values of LP intensity as plants from control (35.1-98.9 nmol MDA g-1 fresh weight). There were no significant differences in LP intensity between control and treatments within the sampling stage, however the amount of MDA accumulated during the vegetation period (up to 60%), possibly because of the developmental processes in soybean and maize. As for seed yield (t ha-1), both investigated species had 5-7% higher yield when inoculated with MIX inoculum, which highlighted the coupled inoculation as possible potent biofertilizer in soybean and maize organic production.
- Published
- 2016
23. Effect of in vitro induced water deficit on lipid peroxidation intensity and antioxidant capacity of sugar beet
- Author
-
Nagl, Nevena, Nagl, Nevena, Kiprovski, Biljana, Taski-Ajdukovic, Ksenija, Ćurčić, Živko, Malenčić, Djordje, Nagl, Nevena, Nagl, Nevena, Kiprovski, Biljana, Taski-Ajdukovic, Ksenija, Ćurčić, Živko, and Malenčić, Djordje
- Abstract
Accompanied by oxidative stress as a signal to activate defense mechanisms, various abiotic factors induce peroxidation of membrane lipids. The aim of this research was to analyse changes in lipid peroxidation (LP) intensity and antioxidant capacity of selected sugar beet genotypes during micropropagation under the conditions of water deficit. Performed research represents a part of a more comprehensive study, the purpose of which is understanding the difference in acclimation to drought as the major abiotic factor limiting sugar beet production in Serbia. The best solution for overcoming this obstacle in successful sugar beet growing is breeding for drought tolerance. Tested sugar beet genotypes were grown and multiplied in vitro on standard nutrient medium. Axillary shoots were placed on the micropropagation media with 3% and 5% PEG for 28 days, which caused physiological drought stress. All genotypes could be divided into three groups according to the level of LP, however only two of eight genotypes had significantly higher LP intensity during drought conditions when compared to their controls (10.1-29.1%), as well as significantly reduced antioxidant activity (22.2-59.9%). A slight decrease in LP intensity and enhanced antioxidant capacity (up to 92.9%) in comparison to control were recorded in treatments of the rest of investigated genotypes. As for correlation between tested biochemical parameters and growth conditions, all genotypes differed in their response to PEG concentration. Obtained results showed that LP intensity and antioxidant capacity could be used as biochemical parameters in assessing drought tolerance of individual genotypes in further selection of sugar beet.
- Published
- 2015
24. Biohemijska i fiziološka karakterizacija klonovatopole (Populus spp.) u procesu fitoekstrakcije bakra, nikla i kadmijuma
- Author
-
Mimica-Dukić, Neda, Štajner, Dubravka, Malenčić, Djordje, Orlović, Saša, Orčić, Dejan, Kebert, Marko, Mimica-Dukić, Neda, Štajner, Dubravka, Malenčić, Djordje, Orlović, Saša, Orčić, Dejan, and Kebert, Marko
- Abstract
Predmet ovog istaživanja bio je ispitivanje uticaja jona tri teška metala (Ni2+, Cu2+ i Cd2+) u dve toksične koncentracije u zemljištu na fiziološke i biohemijske karakteristike odabranih klonova topola, M1, B229 i Pe 19/66. Ispitan je i potencijal odabranih klonova topola da vrše fitoekstrakciju-akumulaciju teških metala iz zemljišta u svoje nadzemne delove što dovodi do dugoročnog uklanjanja ovih perzistentnih polutanata iz životne sredine. Takođe, ispitan je uticaj teških metala na antioksidantni potencijal, sposobnost neutalizacije slobodnih radikala, aktivnosti antioksidantih enzima kao i na sadržaj slobodnih i konjugovanih poliamina (Put, Spm, Spd), određenih HPLC analizom, i sadržaj biljnih hormona poput indol-3-sirćetne kiseline i abscisinske kiseline, određenih GC/MS analizom, u listovima i korenovima klonova topola., The aim of this study was to estimate and compare phytoextraction capacities of three poplar clones (M1, B229 and Pe 19/66) in soil. Furthermore, the goal was to assess different biological responses among the poplar clones during exposure to different concentration of three heavy metal ions (Ni2+, Cu2+ i Cd2+). In order to track changes in poplars’mineral, physiological, biochemical and antioxidant status during the abiotic stress, quantification of physiological properties, free and conjugated polyamines, total phenolics as well as quantification of phytohormones (indol-3-acetic and abscisic acid) was done. Furthermore, assessment of antioxidant potential by tracking radical scavenger capacities (RSC) against DPPH, ABTS, OH and NO radicals and by measuring enzymes activities (SOD, GSH-Px, GPx, GR) in vitro was performed in root and leaves of poplar clones.
- Published
- 2014
25. THE INFLUENCE OF MATURITY STAGE AND EXTRACTION SOLVENTS ON PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF THREE SWEET CHERRY CULTIVARS.
- Author
-
PRVULOVIĆ, Dejan, MALENČIĆ, Djordje, LJUBOJEVIĆ, Mirjana, BARAĆ, Goran, and OGNJANOV, Vladislav
- Subjects
- *
SWEET cherry , *FRUIT varieties , *FRUIT development , *PHENOL content of fruit , *PLANT extracts , *ANTIOXIDANTS - Abstract
The effects of two extracting solvents (70% acetone and 70% ethanol) and maturity stage (semi ripe and ripe) on the phenolic content and antioxidant activity of fruits of three sweet cherry cultivars (Burlat, New Star and Peter) were investigated. Results showed that extraction solvent did not have significant effects on total phenolics (TP), tannins (TT) and flavonoids (TF) content and antioxidant activity (1,2-diphenyl-2-picryl-hydrazyl (DPPH) assay, ferricreducing antioxidant power (FRAP) assay, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay, total antioxidant activity (TAA) by phosphomolybedenum complex formation method and reducing power (RP) assay) in dried fruits of sweet cherry. The results did not showed significant changes in phenolic content and antioxidant capacity of fruits during the ripening. Among the investigated sweet cherry fruits, Peter cultivar contained the highest amounts of all groups of phenolics, followed by Burlat and New Star. TP in fruits ranged from 10.90 (ripe New Star, ethanol extract) to 28.92 (semi ripe Peter, acetone extract) mg gallic acid equivalents (GAE)/g dry weight (DW). The highest amount of TF in fruits was detected in ethanol extract of semi ripe Peter cultivar (12.97 mg quercetin equivalents (QE)/g DW), while the lowest content was found in ethanol extracts of semi ripe New Star cultivar (7.80 mg QE/g DW). The examined cultivars possess a high antioxidant capacity, and all measured phenolic groups were highly correlated with performed antioxidant assays. The antioxidant activity values obtained with DPPH in the dry fruits (ranging from 7.68 to 13.29 mg trolox equivalents (TE)/g DW) were comparable to those obtained with FRAP 3.69 to 13.28 mg TE/g DW). [ABSTRACT FROM AUTHOR]
- Published
- 2016
26. Changes in antioxidant capacity of soybean and maize seedlings as a response to Rhizoctonia solani Kühn infection
- Author
-
Kiprovski, Biljana, Kiprovski, Biljana, Malenčić, Djordje, Popović, Milan, Stojšin, Vera, Budakov, Dragana, Kiprovski, Biljana, Kiprovski, Biljana, Malenčić, Djordje, Popović, Milan, Stojšin, Vera, and Budakov, Dragana
- Abstract
Changes in enzymatic and non-enzymatic antioxidant systems in leaves and roots of soybean and maize seedlings were studied after inoculation with Rhizoctonia solani Kühn, AG 2-2 IIIB. After the infection with R. solani, increase in superoxide anion (O2 .- ) scavenging activity was recorded only in leaves and roots of soybean plants. Hydroxyl radical (.OH), the production of which was remarkably increased in infected soybean seedlings, were invariably produced in infected maize seedlings in comparison to control. Furthermore, superoxide-dismutase activity increased only in infected maize roots, which could point to enhanced O2 .-dismutation. Nevertheless, lipid peroxidation intensity was notably recorded only in soybean plants, which could lead to presumption that .OH was successfully neutralized in maize seedlings and that membrane lipids were prevented from peroxidation with .OH, potentially the most responsible for these processes. Peroxidase were significantly active in infected leaves compared to control in both species, especially in soybean. Naturally, ROS accumulation induced significantly enhanced phenylalanin ammonialyase (PAL) activity, which escalated phenolic compounds synthesis in maize seedlings. Ability to accumulate phenolics, under the condition of pathogen attack, correlated with the results for the DPPH-assay that determines these maize plants in active group1.
- Published
- 2011
27. Correlation between lipid peroxidation and phenolics content in leaves and roots of sugar beet infected with Rhizoctonia solani
- Author
-
Kiprovski, Biljana, primary, Malenčić, Djordje, additional, Popović, Milan, additional, Stojšin, Vera, additional, Budakov, Dragana, additional, Ćurčić, Živko, additional, and Danojević, Dario, additional
- Published
- 2013
- Full Text
- View/download PDF
28. Whole grain phenolics and antioxidant activity of Triticum cultivars and wild accessions.
- Author
-
MALENČIĆ, DJORDJE, KIPROVSKI, BILJANA, BURSIĆ, VOJISLAVA, VUKOVIÆ, GORICA, HRISTOV, NIKOLA, and KONDIÆ-ŠPIKA, ANKICA
- Subjects
- *
WHOLE grain foods , *PHYSIOLOGICAL effects of phenols , *ANTIOXIDANTS , *WHEAT varieties , *PLANT genomes - Abstract
The contents of soluble free phenolics (SP), PVPP-bound tannins (PVPP-P), flavonoids (TF) and proanthocyanidins (PA), as well as the DPPH- -radical scavenging capability, of the whole grain of 17 different Triticum spp. accessions were analyzed. The contents of SP ranged from 976 to 2927 μg CE g-1, of PVPP-P from 335 to 1412 μg CE g-1 and of TF from 0.16 to 1.12 μg RE g-1 of dry whole grain. The presence of PA in the whole grains was not established. The values for DPPH-radical scavenging activity were rather moderate, under 40% of neutralized radicals. A positive correlation between DPPH-activity and analyzed phenolics was recorded in twelve of the seventeen genotypes (r from 0.73 to 0.99). trans-Cinnamic acid was the major compound among acids and quercetin among flavonoids found in the wheat varieties. The data obtained should enable the selection of the accessions rich in biologically active compounds that could further be processed into functional food with possible health benefits associated with higher antioxidant properties. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
29. Polyphenol Content and Antioxidant Properties of Colored Soybean Seeds from Central Europe
- Author
-
Malenčić, Djordje, primary, Cvejić, Jelena, additional, and Miladinović, Jegor, additional
- Published
- 2012
- Full Text
- View/download PDF
30. Changes in antioxidant systems in soybean as affected by Sclerotinia sclerotiorum (Lib.) de Bary
- Author
-
Malenčić, Djordje, primary, Kiprovski, Biljana, additional, Popović, Milan, additional, Prvulović, Dejan, additional, Miladinović, Jegor, additional, and Djordjević, Vuk, additional
- Published
- 2010
- Full Text
- View/download PDF
31. Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin
- Author
-
Tepavčević, Vesna, primary, Atanacković, Milica, additional, Miladinović, Jegor, additional, Malenčić, Djordje, additional, Popović, Jovan, additional, and Cvejić, Jelena, additional
- Published
- 2010
- Full Text
- View/download PDF
32. Phenolic Content and Antioxidant Properties of Soybean (Glycine max (L.) Merr.) Seeds
- Author
-
Malenčić, Djordje, primary, Popović, Milan, additional, and Miladinović, Jegor, additional
- Published
- 2007
- Full Text
- View/download PDF
33. ALLELOPATHIC EFFECTS AND INSECTICIDAL ACTIVITY OF Salvia sclarea L.
- Author
-
ŠUĆUR, JOVANA, POPOVIĆ, ALEKSANDRA, PETROVIĆ, MILOŠ, ANAČKOV, GORAN, MALENČIĆ, DJORDJE, and PRVULOVIĆ, DEJAN
- Subjects
ALLELOPATHIC agents ,LAMIACEAE ,SALVIA ,ANIMAL health ,PEROXIDATION - Abstract
Extensive use of synthetic pesticides has negative effects on the environment and on human and animal health. Knowledge of allelopathic interactions could provide powerful tools for a better exploitation of natural resources in the management of weeds and insects without using herbicides and insecticides. Therefore effect of two concentrations (0.1% and 0.2%) of Salvia sclarea L. aqueous extract on lipid peroxidation process, as well as the activity of the antioxidant enzymes (superoxid dismutase, guaiacol peroxidase, pyrogallol peroxidase and catalase) in leaves and roots of pepper (Capsicum annum L.) and black nightshade (Solanum nigrum L.) seedlings were examined. Our results showed that lower concentration of S. sclarea aqueous extract induced lipid peroxidation in black nightshade roots. The second aim was to evaluate effectiveness of aqueous extract as contact toxicant against whitefly (Trialeurodes vaporariorum). It was observed that aqueous extract with concentration of 0.1% showed toxic effect with 56.66% mortality. [ABSTRACT FROM AUTHOR]
- Published
- 2015
34. Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum.
- Author
-
Malenčić, Djordje, Cvejić, Jelena, Tepavčević, Vesna, Bursać, Mira, Kiprovski, Biljana, and Rajković, Miloš
- Abstract
Soybean [ Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
35. Anatomical characteristics and antioxidant ability of Centaurea sadleriana reveals an adaptation towards drought tolerance.
- Author
-
Luković, Jadranka, Malenčić, Djordje, Zorić, Lana, Kodranov, Miroslava, Karanović, Dunja, Kiprovski, Biljana, and Boža, Pal
- Abstract
The lamina, main vein and peduncle anatomical properties of Centaurea sadleriana Janka plants from two populations, were examined using light and scanning electron microscopy. The indumentum was comprised of glandular and non-glandular trichomes of two types. The leaves were amphistomatic, isolateral, with strongly developed palisade tissue. Secretory ducts were observed along the phloem or sclerenchyma of large vascular bundles. Collenchyma alternated with chlorenchyma in the main vein and peduncle. Large groups of strongly lignified sclerenchyma were present along the phloem of peduncle vascular bundles. These features, together with thickened walls of epidermal cells and cuticle, numerous trichomes and thick-walled parenchyma in the perimedullar zone, were perceived as a xeromorphic peduncle structural adaptation. Non-enzymatic antioxidant compounds of phenolic origin were detected in small amounts and their respective content was higher in leaves compared to inflorescences. Compounds of phenolic orgin showed positive correlation with total potenial of antioxidant activity indicated by the DPPH assay. Greater total quantity of polyphenols and tannins was detected in leaves of plants from Zobnatica locality, while leaves of plants from Rimski Sanac were characterized by higher content of total flavonoids and proantocyanidins. Phytochemical analysis showed that dominant secondary biomolecules in inflorescences were phenolic pigments including anthocyanins and leucoanthocyanins, and free quinones in leaves. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
36. PHENOLIC CONTENT AND ANTIOXIDANT CAPACITY OF SWEET AND SOUR CHERRIES.
- Author
-
PRVULOVIĆ, DEJAN, POPOVIĆ, MILAN, MALENČIĆ, DJORDJE, LJUBOJEVIĆ, MIRJANA, BARAĆ, GORAN, and OGNJANOV, VLADISLAV
- Subjects
SWEET cherry ,SOUR cherry ,PHENOLIC acids ,PHENOLS ,BIOLOGICAL assay of antioxidants ,TANNINS ,FLAVONOIDS ,ANTHOCYANINS - Abstract
Sweet and sour cherries are a valuable natural source of somebioactive compounds important in human health preservation. Total phenolics,tannins, flavonoids and anthocyanins, and antioxidant capacity in a fruits of atwo selected sweet cherry genotypes (Szomolyai Gombolyii and Valerij Cskalov)and 2 sour cherry cultivars (Érdi bőtermő and Kántorjánosi) were investigated.Total phenolic content ranged from 76.05 up to 301.19 mg gallic acidequivalents/100 g fresh fruit weight and total tannins content ranged from32.33 to 236.61 mg gallic acid equivalents/100 g fresh fruit weight. Totalflavonoids were within the range 49.47-70.27 mg of rutin equivalents/100 gfresh fruit weight and total anthocyanins content were between 16.86 and51.16 mg cyanidin 3-glucoside equivalents/100 g fresh fruit weight. Antioxidantactivity of sweet and sour cherries is correlated with the total phenolics andtotal tannins content, and partially related with total anthocyanins, but not withthe total flavonoids. Fruits of sour cherries contains more phenolics than fruitsof sweet cherries and possess more potent antioxidant activity. [ABSTRACT FROM AUTHOR]
- Published
- 2012
37. Cholic acid changes defense response to oxidative stress in soybean induced by Aspergillus niger.
- Author
-
Malenčić, Djordje, Kevrešan, Slavko, Popović, Milan, Štajner, Dubravka, Popović, Boris, Kiprovski, Biljana, and Djurić, Simonida
- Abstract
The oxidative stress and antioxidant systems in soybean leaves and roots infected with plant pathogen Aspergillus niger were studied following treatment with different concentrations of cholic acid. Several oxidative stress parameters were analyzed: production of superoxide (O) and hydroxyl radicals (OH), lipid peroxidation (LP), and superoxide dismutase (SOD; EC 1.15.1.1) activity, as well as the content of reduced glutathione (GSH). Results showed that inoculation with A. niger led to the increase of O production and GSH quantities in leaves and OH in roots. The highest activity of SOD occured in infected plants treated with cholic acid in concentrations of 40 and 60 mg L which ultimately led to a decrease in O production. Inoculation with Aspergillus in combination with elevated cholic acid concentrations also increased OH production which is correlated with increased LP. These results may support the idea of using cholic acid as an elicitor to trigger hypersensitive response in plant cells. Use of cholic acid may also actively contribute to soybean plants defense response against pathogen attack. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
38. Anatomical characteristics and antioxidant properties of Euphorbia nicaeensis ssp. glareosa.
- Author
-
Luković, Jadranka, Malenčić, Djordje, Zorić, Lana, Kiprovski, Biljana, Merkulov, Ljiljana, and Boža, Pal
- Abstract
Anatomical analyses found that leaves of Euphorbia nicaeensis ssp. glareosa are isolateral, amphistomatous, with two layers of palisade cells on the adaxial and one on the abaxial side. Laticifers are present by vascular bundles, in palisade and spongy tissue. Stem laticifers are located in the pericyclic ring, adjacent to the phloem, in cylinder parenchyma and medullar rays. The structure of pleiochasium and dichasium peduncle is similar to the stem structure. Plants from typical steppe habitat show more xeromorphic features. Phytochemical screening of extracts showed presence of catecholes, flavonoids, tannins, saponins, free quinone derivatives and absence of anthocyanins, leucoanthocyanins, alkaloids, steroid compounds and essential oils. Our results showed that the examined taxon was partially susceptible to the action of reactive oxygen species, such as O
2 ·− and ·OH. The higher quantities of ROS thus provoked an antioxidative response from the plant, both in an enzymatic and non-enzymatic manner. Stable anatomical structure, presence and distribution of laticifers and effective antioxidant properties when exposed to ROS, make Euphorbia nicaeensis subsp. glareosa potentially interesting for further pharmaceutical and phytochemical examinations. [ABSTRACT FROM AUTHOR]- Published
- 2009
- Full Text
- View/download PDF
39. Polyphenol contents and antioxidant activity of soybean seed extracts
- Author
-
Malenčić, Djordje, Maksimović, Zoran, Popović, Milan, and Miladinović, Jegor
- Subjects
- *
SOYBEAN , *ANTIOXIDANTS , *POLYPHENOLS , *SEEDS , *TANNINS , *ANTHOCYANIDINS , *FLAVONOIDS - Abstract
The antioxidant activity and contents of various polyphenol classes in the seeds of 20 soybean hybrids were evaluated. Total polyphenols, tannins and proanthocyanidins were determined after extraction of plant material with 70% aqueous acetone. In addition, flavonoid content was determined. Antioxidant activity of seed extracts was evaluated by DPPH free radical scavenging activity assay. A positive linear correlation between antioxidant activity and contents of total phenols, tannins and proanthocyanidins was established. The highest antioxidant activity was observed in the extracts of hybrids which have higher levels of all polyphenol classes examined. The most of the single-cross hybrids were poor in tannins which recommend them as good source for ensiled livestock feed. Results suggested that polyphenol content should be considered as an important feature of the soybean seed. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
40. Effects of linuron and dimethenamid on antioxidant systems in weeds associated with soybean.
- Author
-
Malenčić, Djordje, Miladinović, Jegor, and Popović, Milan
- Abstract
Changes in antioxidant systems in soybean and associated weeds ( Ambrosia artemisiifolia L., Chenopodium album L., Convolvulus arvensis L and Sinapis arvensis L.) were studied in relation to treatment with herbicides linuron and dimethenamid in the field experiment. Differences in the total superoxide dismutase (SOD) and catalase (Cat) activities were observed in plants after application of herbicide formulation. Quantities of superoxide (O
2 .-) and hydroxyl (·OH) radicals and malonyldialdehyde (MDA), reduced glutathione (GSH) and total polyphenols content were also determined. In addition to this, potential antioxidant activity of the plant ethanolic extracts were assessed based on the scavenging activity of stable DPPH free radicals. Results obtained suggest that plants investigated 1) expressed different antioxidant systems in response to herbicide treatment; 2) enzymatic and non-enzymatic protective mechanisms were complementary; 3) some weed species showed distinctive and combined activity of several biochemical parameters, such as Ambrosia artemisiifolia. [ABSTRACT FROM AUTHOR]- Published
- 2008
- Full Text
- View/download PDF
41. Effect of in vitro induced water deficit on lipid peroxidation intensity and antioxidant capacity of sugar beet
- Author
-
Nagl, Nevena, Biljana Kiprovski, Taski-Ajdukovic, Ksenija, Ćurčić, Živko, Malenčić, Djordje, Uzelac, Branka, and Šiler, Branislav
- Subjects
biochemical markers ,micropropagation ,abiotic stress ,fungi ,drought stress ,food and beverages ,antioxidant activity ,sugar beet - Abstract
Accompanied by oxidative stress as a signal to activate defense mechanisms, various abiotic factors induce peroxidation of membrane lipids. The aim of this research was to analyse changes in lipid peroxidation (LP) intensity and antioxidant capacity of selected sugar beet genotypes during micropropagation under the conditions of water deficit. Performed research represents a part of a more comprehensive study, the purpose of which is understanding the difference in acclimation to drought as the major abiotic factor limiting sugar beet production in Serbia. The best solution for overcoming this obstacle in successful sugar beet growing is breeding for drought tolerance. Tested sugar beet genotypes were grown and multiplied in vitro on standard nutrient medium. Axillary shoots were placed on the micropropagation media with 3% and 5% PEG for 28 days, which caused physiological drought stress. All genotypes could be divided into three groups according to the level of LP, however only two of eight genotypes had significantly higher LP intensity during drought conditions when compared to their controls (10.1-29.1%), as well as significantly reduced antioxidant activity (22.2-59.9%). A slight decrease in LP intensity and enhanced antioxidant capacity (up to 92.9%) in comparison to control were recorded in treatments of the rest of investigated genotypes. As for correlation between tested biochemical parameters and growth conditions, all genotypes differed in their response to PEG concentration. Obtained results showed that LP intensity and antioxidant capacity could be used as biochemical parameters in assessing drought tolerance of individual genotypes in further selection of sugar beet.
42. Total and individual phenolics in wild and cultivated elderberry during development
- Author
-
Biljana Kiprovski, Malenčić, Djordje, Ljubojević, Mirjana, Ognjanov, Vladislav, Veberič, Robert, and Mikulič-Petkovšek, Maja
- Subjects
unripe and ripe fruits ,zova ,Sambucus nigra ,total phenolics ,individual phenolics ,elderberry ,fruits ,leaves ,cultivated elderberry - Abstract
Compounds which add to bioactivity of elderberry (Sambucus nigra L.) are mostly phenolic compounds. One wild edible and two cultivated genotypes (’Haschberg’ and ’Ljubostinja’ selections) have been chosen. Leaves and fruits were collected in four stages (April-September). Total phenolics (TP) content was determined from MeOH extracts by Folin-Ciocalteau method while determination of individual compounds was performed using HPLC-DAD-ESI-MSn analysis with a DAD at 280, 350 and 530 nm. TP content in leaves varied through developmental stages, being higher in II and III stage. Leaves and fruits (both unripe and ripe) of ’Ljubostinja’ plants had higher content of TP compared to wild ripe fruits (1.5-fold). Leaves had caffeic acid derivatives (0.3-0.7% in wild, 0.2-0.3% in ’Haschberg’ and 0.4-0.6% in ’Ljubostinja’ in dry weigh) and caffeoylquinic acid derivatives, while fruits had more caffeoylquinic acid derivatives, particularly unripe fruits. The major phenolic acid present in leaves, especially during the II stage of development, was chlorogenic acid (approx. 4% dw) and the same was recorded in fruits (0.03% dw in unripe fruits of wild plants). Content of phenolic acids decreased during ripening of elderberry fruits and the similar was detected in the leaves.
43. Effect of extraction solvents on the antioxidant activity of industrial hemp extracts
- Author
-
Prvulović, Dejan, Gvozdenac, Sonja, Peić Tukuljac, Marijana, Malenčić, Djordje, Biljana Kiprovski, Sikora, Vladimir, and Latković, Dragana
- Subjects
phenolics ,flavonoids ,antioxidant capacity ,Cannabis sativa ,hemp ,polyphenols - Abstract
The objective of this study was to determine the content of phenolic compounds (total phenolics, total tannins and total flavonoids) and antioxidant capacity by six different assays in industrial hemp (‘Helena’ variety) extracted by four different extraction solvents: 70% methanol, 70% ethanol, 70% acetone and water. Out of the four solvent mixtures evaluated in the current study for the extraction of phenolic compounds, the use of 70% acetone yielded to the highest total contents of phenolics and exhibited the highest antioxidant activity in all performed assays.
44. Changes in polyphenols content in soybean and Datura stramonium after treatment with herbicides and Delfan Plus
- Author
-
Malenčić, Djordje, Biljana Kiprovski, Prvulović, Dejan, Rajković, Miloš, Malidža, Goran, Uzelac, Branka, and Šiler, Branislav
- Subjects
abiotic stress ,herbicides ,antioxidants ,phenolics ,fungi ,biostimulators ,food and beverages ,antioxidant capacity ,soybean ,polyphenols - Abstract
The most common herbicide combination for weed prevention in soybean is Pulsar 40 and Harmony 75WG, but when applied during hot days these herbicides may induce oxidative stress in plants. Delfan Plus® is a biostimulator with high amino acid content which can be used in combination with herbicides. Manufacturer advertises it as a supplement for faster protein synthesis and thus, overcoming stress conditions caused by high and low temperatures, drought, herbicides etc. In order to investigate the ability of Delfan Plus® to mitigate the effect of herbicides on soybean plants (and its weed species Datura stramonium L.), we analyzed the accumulation of polyphenolic compounds (total polyphenols, tannins, flavonoids and proanthocyanidins), as well as phytotoxicity and grain yield. In addition, we determined the antioxidant capacity of plant extracts in DPPH and NBT-tests. Soybean cultivar Sava was treated with Pulsar 40 + Harmony 75WG (1 L ha-1 + 8 g ha-1 and 2 L ha-1 + 16 g ha-1, respectively), with or without Delfan Plus® (1 L ha-1 and 2 L ha-1, respectively). Biochemical parameters analyzed showed that Delfan plus® did not decrease the level of stress in plants. Most parameters increased their values 7 days after the treatment, compared to day 1, which was expected. Specimens of D. stramonium were especially stressed after herbicide treatment. Although soybean yield was somewhat higher after the treatment with normal dose of herbicides and Delfan plus® (3.28 t ha-1), there were no statistical differences between the treatments. Hence, the question of the application of this biostimulator in agronomic practice may be raised.
45. Changes in antioxidant capacity of soybean and maize seedlings as a response to Rhizoctonia solani Kühn infection
- Author
-
Biljana Kiprovski, Malenčić, Djordje, Popović, Milan, Stojšin, Vera, and Budakov, Dragana
- Subjects
antioxidants ,phenolics ,fungi ,seedlings ,enzymatic activity ,food and beverages ,oxidative stress ,lipid peroxidation ,soybean ,maize - Abstract
Changes in enzymatic and non-enzymatic antioxidant systems in leaves and roots of soybean and maize seedlings were studied after inoculation with Rhizoctonia solani Kühn, AG 2-2 IIIB. After the infection with R. solani, increase in superoxide anion (O2 .- ) scavenging activity was recorded only in leaves and roots of soybean plants. Hydroxyl radical (.OH), the production of which was remarkably increased in infected soybean seedlings, were invariably produced in infected maize seedlings in comparison to control. Furthermore, superoxide-dismutase activity increased only in infected maize roots, which could point to enhanced O2 .-dismutation. Nevertheless, lipid peroxidation intensity was notably recorded only in soybean plants, which could lead to presumption that .OH was successfully neutralized in maize seedlings and that membrane lipids were prevented from peroxidation with .OH, potentially the most responsible for these processes. Peroxidase were significantly active in infected leaves compared to control in both species, especially in soybean. Naturally, ROS accumulation induced significantly enhanced phenylalanin ammonialyase (PAL) activity, which escalated phenolic compounds synthesis in maize seedlings. Ability to accumulate phenolics, under the condition of pathogen attack, correlated with the results for the DPPH-assay that determines these maize plants in active group1.
46. Sugars and organic acids content in unripe and ripe fruits of Sambucus nigra
- Author
-
Biljana Kiprovski, Malenčić, Djordje, Ognjanov, Vladislav, Veberič, Robert, Hudina, Metka, and Mikulič-Petkovšek, Maja
- Subjects
unripe and ripe fruits ,sugars ,Sambucus nigra ,organic acids ,elderberry ,cultivated elderberry - Abstract
Elderberry (Sambucus nigra L.) is a common plant used for its medicinal and nutritional properties, as for the beverages, jams, liqueurs, flavourings etc. Plant material examined were leaves and fruits of wild edible, and cultivated elderberry plants (’Haschberg’ and ’Ljubostinja’ selections). Determination of organic acids and sugars was performed using high-performance liquid chromatography. Organic acids found in the tested fruit samples were citric, tartaric, malic, quinic, shikimic and fumaric acids, out of which quinic acid content was the highest in unripe, while citric acid content was the highest in ripe fruits. Wild plants had higher contents of organic acids in unripe fruits (16.7 g 100 g-1dw) than cultivated (’Haschberg’ 6.6, and ’Ljubostinja’ 6.3 g 100 g-1dw). ’Ljubostinja’ selection had significantly higher organic acids content (10.2 g 100 g-1dw) in ripe fruits compared to wild specimen and ’Haschberg’ plants which had similar organic acids content (7.0 and 7.4 g 100 g-1dw). The major organic acid in ripe elderberry fruits was citric acid. When it comes to sugars content the presence of sucrose, glucose, fructose and sorbitol was established. Wild specimen had significantly higher sugars content (2.2 in unripe, and 8.4 g 100 g-1dw in ripe fruits).
47. Fitohemijski sastav, lekoviti potencijal i senzorne karakteristike sorti vrsta Brassica oleracea L. i Brassica rapa L. (Brassicaceae) iz organskog i konvencionalnog sistema proizvodnje
- Author
-
Bošković, Svetlana, Mimica-Dukić, Neda, Orčić, Dejan, Malenčić, Djordje, Beara, Ivana, and Četojević-Simin, Dragana
- Subjects
antioksidanti ,organic ,Brassica oleracea, Brassica rapa, broccoli, cauliflower, kohlrabi, Savoy cabbage, red cabbage, white cabbage, Nappa cabbage, organic, convenventional, antioxidants, antimicrobial activity, cytotoxicity ,antimikrobna aktivnost ,organski ,B. rapa ,convenventional ,konvencionalni ,citotoksičnost ,Nappa cabbage ,karfiol ,brokoli ,broccoli ,Savoy cabbage ,antimicrobial activity ,red cabbage ,kelj ,cauliflower ,Brassica rapa ,kohlrabi ,kineski kupus ,white cabbage ,B. oleracea ,antioxidants ,beli kupus ,cytotoxicity ,Brassica oleracea ,crveni kupus ,B. oleracea, B. rapa, brokoli, karfiol, keleraba, kelj, crveni kupus, beli kupus, kineski kupus, organski, konvencionalni, antioksidanti, antimikrobna aktivnost, citotoksičnost ,keleraba - Abstract
U ovom radu su ispitani hemijski sastav i biološka aktivnost vrsta Brassica oleracea i Brassica rapa poreklom iz organskog i konvencionalnog sistema proizvodnje. Ispitivanja hemijskog sastava su obuhvatila određivanje volatilnih komponenata primenom headspace GC-MS tehnike, kvalitativnu analizu sokova ispitivanih vrsta pomoću LC-DAD-MS/MS tehnike, kvantifikaciju odabranih fenolnih jedinjenjapomoću LC-MS/MS tehnike i spektrofotometrijsko određivanje sadržaja ukupnih glukozinolata, fenola i flavonoida i sadržaja ukupnih monomernih antocijana. Biološka aktivnost povrća je ispitana određivanjem antioksidantnog potencijal, antimikrobne i antitumorske aktivnosti sokova. Dobijeni rezultati pokazuju da su glavne volatilne komponente ispitivanih vrsta degradacioni proizvodi masnih kiselina i organosumpornih jedinjenja od kojih neke od identifikovanih komponenata predstavljaju ključne mirisne komponente. Na osnovu njihove distribucije nije bilo moguće utvrditi uticaj sistema proizvodnje na miris i ukus ispitivanih vrsta. Pomoću LC-DAD-MS/MS i LC-MS/MS tehnike je utvrđeno prisustvo primarnih metabolita, slobodnih hidroksibenzoevih i hidroksicimetnih kiselina, derivate hinske kiselinesa hidroksicimetnim kiselinama, glikozida flavonola, biflavonoida, i kumarina u sokovima spitivanih vrsta. Pored toga u svim sokovima subili prisutni kondenzacioni proizvodi degradacionih proizvoda indolnih glukozinolata sa askorbinskom kiselinom- askorbigen i metoksiaskorbigen. U sokovima crvenog kupusa su bili prisutni antocijani. Najveći sadržaj ukupnih glukozinolata je određen u brokoliju, dok je crveni kupus sadržao najveći sadržaj ukupnih fenola ukupnih flavonoida kao i hinske kiseline. Crveni kupus je bio varijetet koji je ispoljio najviši antioksidantni kapacitet u svim primenjenim testovima, brokoli i karfiol su ispoljili najjaču antimikrobnu aktivnost, dok je kelj bio varijetet sa najjačim antitumorskim delovanjem. Na osnovu analize hemijskog sastava i biološke aktivnosti vrsta B. oleracea i B. rapa može se zaključiti da u zavisnosti od primenjenog tipaproizvodnje mogu biti poboljšane neke od karakteristika ispitivanih vrsta i da je uticaj sistema proizvodnje značajan za pojedine varijetete, a nikako za celu vrstu. U konačnom obliku može se zaključiti da bi potencijalna kombinacija sokova crvenog kupusa, brokolija, karfiola i kelja mogla ostvariti značajno hemopreventivno i hemoterapeutsko delovanje, ispoljavajući istovremeno antioksidantnu, antimikrobnu i antitumorsku aktivnost., Chemical composition and biological activity of Brassica oleracea and B. rapa species were investigated in the present work. Investigation of chemical composition encompassed determination of volatile compounds by headspace GC-MS technique, qualitative analysis of juices of investigated vegetables by LC-DAD-MS/MS technique, quantification of selected phenolic compounds by LC-MS/MS technique and spectrophotometric determination of total content of glucosinolates, phenolic compounds and flavonoids and total content of monomeric anthocyanes. Biological activity of vegetables was investigated by determination of antioxidant capacity, antimicrobial and antitumorigenic effect of juices. Obtained results showed that main volatile compounds were degradation products of fatty acids and organo-sulphur compounds among which some of the identified components were the key aroma compounds. Based on their distribution it was not possible to evaluate influence of cropping system on aroma and taste of investigated species. By LC-DAD-MS/MS and LC-MS/MS techniques, it was determined the presence of primary metabolites, freehydroxybenzoic and hydroxycinnamic acids, derivatives of quinic acid and hidroxicinnamic acids, flavonol glucosides, biflavnoids and a cumarin in juices of the investigated species. Furthermore, condensation products of degradation products of indole glucosinolates with ascorbic acid- ascorbigen and metoxiascorbigen were present in all juices. Anthocyanes were present in juices of red cabbage. The highest total content of glucosinolates was determined in broccoli, while red cabbage had the highest total phenolic content, total flavonoid content, as well as content of qunic acid. Red cabbage was a variety that showed the highest antioxidant capacity measured by different assays. Broccoli and cauliflower expressed the highest antimicrobial potential, while Savoy cabbage was a variety with the highest antitumor effect. Investigation of chemical composition and biological activity showed that applied cropping conditions may be useful for improving some of the characteristics of investigated species and significance of cropping system only for particular varieties, but not for the whole species in general. As the conclusion, potential combination of juices of red cabbage, broccoli, cauliflower and Savoy cabbage could be potent chemopreventive and chemotherapeutic agent, expressing simultaneously antioxidant, antimicrobial and antitumor activity.
- Published
- 2016
48. Biohemijska i fiziološka karakterizacija klonovatopole (Populus spp.) u procesu fitoekstrakcije bakra, nikla i kadmijuma
- Author
-
Kebert, Marko, Mimica-Dukić, Neda, Štajner, Dubravka, Malenčić, Djordje, Orlović, Saša, and Orčić, Dejan
- Subjects
reactive oxygen species ,Poplars, phytoextraction, heavy metals, reactive oxygen species, abscisic acid (ABA), indol-3 acetic acid (IAA), antioxidant activity, polyamines, AAS, HPLC, GC-MS ,polyamines ,antioksidantna aktivnost ,antioxidant activity ,poliamini ,AAS ,phytoextraction ,indol-3 sirćetna kiselina (IAA) ,GC/MSlizom ,Poplars ,reaktivne kiseonične vrste, abscisinska kiselina (ABA), indol-3 sirćetna kiselina (IAA), antioksidantna aktivnost, poliamini, AAS, HPLC, GC/MSlizom, u listovima i korenovima klonova topola ,u listovima i korenovima klonova topola ,indol-3 acetic acid (IAA) ,abscisic acid (ABA) ,reaktivne kiseonične vrste ,HPLC ,GC-MS ,abscisinska kiselina (ABA) ,heavy metals - Abstract
Predmet ovog istaživanja bio je ispitivanjeuticaja jona tri teška metala (Ni2+, Cu2+i Cd2+)u dve toksične koncentracije u zemljištu nafiziološke i biohemijske karakteristikeodabranih klonova topola, M1, B229 i Pe 19/66.Ispitan je i potencijal odabranih klonova topolada vrše fitoekstrakciju-akumulaciju teškihmetala iz zemljišta u svoje nadzemne delove štodovodi do dugoročnog uklanjanja ovihperzistentnih polutanata iz životne sredine.Takođe, ispitan je uticaj teških metala naantioksidantni potencijal, sposobnostneutalizacije slobodnih radikala, aktivnostiantioksidantih enzima kao i na sadržajslobodnih i konjugovanih poliamina (Put, Spm,Spd), određenih HPLC analizom, i sadržajbiljnih hormona poput indol-3-sirćetne kiselinei abscisinske kiseline, određenih GC/MSanalizom, u listovima i korenovima klonova topola. , The aim of this study was to estimate and compare phytoextraction capacities of threepoplar clones (M1, B229 and Pe 19/66) in soil. Furthermore, the goal was to assess different biological responses among the poplar clones during exposure to different concentration of three heavy metal ions (Ni2+, Cu2+ i Cd2+). In order to track changes in poplars’mineral, physiological, biochemical and antioxidant status during the abiotic stress, quantification of physiological properties, free and conjugated polyamines, total phenolics as well as quantification of phytohormones (indol-3-acetic and abscisic acid) was done. Furthermore, assessment of antioxidant potential by tracking radical scavenger capacities (RSC) against DPPH, ABTS, OH and NO radicals and by measuring enzymes activities (SOD, GSH-Px, GPx, GR) in vitro was performed in root and leaves of poplar clones.
- Published
- 2014
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.