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376 results on '"Makvandi, Manoochehr"'

1. Comprehensive assessment of COVID-19 case fatality rate and influential factors in Khuzestan Province, Iran: a two-year study

Author

Bastani, Mohammad-Navid, Makvandi, Manoochehr, Moradi, Maryam, Haghighi, Somayeh Biparva, Rostami, Maryam, Nasimzadeh, Sepideh, Amiri, Homayoun, Alavi, Seyed Mohammad, Rashno, Mohammad, Mohtadi, Ahmadreza, Yousefi, Farid, Fayezi, Abbas, Mirkarimi, Mohammadreza, Shoushtari, Maryam Haddadzadeh, Zadkarami, Masoud, Balar, Negin, Sameii, Siamak Mirab, and Torabizadeh, Mehdi

Published
2024
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6. Molecular detection of Epstein-Barr virus in paraffin-embedded tissue samples of patients suffering gastric cancer in Ahvaz, Iran: a case-control study.

Author

Gharibzadeh, Mehdi, Jalilian, Shahram, Makvandi, Manoochehr, Seyedian, Seyed Saeid, and Azaran, Azarakhsh

Subjects
EPSTEIN-Barr virus diseases, DIETARY patterns, STOMACH cancer, STOMACH ulcers, IRANIANS
Abstract

Background and Objectives: Gastric cancer (GC) is the third most common cause of cancer-related mortality. Epstein-Barr virus (EBV) is associated with several human tumors. The present research was performed to investigate the prevalence of EBV-associated gastric cancer (EBVaGC) among Iranian patients. Materials and Methods: Seventy cases of gastric cancer and 30 cases of gastric ulcer, all preserved in formalin-fixed paraffin-embedded (FFPE), were examined in a case-control study conducted between 2011 and 2018. The specimens underwent analysis to detect the presence of the EBV genome using a Nested-PCR method targeting EBNA1. Subsequently, samples testing positive for the EBNA1 underwent further testing for the presence of the EBER gene using PCR. Finally, Positive samples were subjected to sequencing. Results: Five out of 70 cases (7%) were found to be positive for EBV based on EBNA1 testing, while all EBNA1 positive samples were negative for EBER. Notably, EBV was not detected in patients with gastric ulcer. The mean age of EBV-positive gastric carcinomas pateints was 64.5 years. Within this group, 60% were male and 40% were female. A higher prevalence of EBV association was observed in diffuse-type cases, with 60% (3 out of 24) testing positive, compared to intestinal-type cases where 40% (2 out of 46) were EBV-positive. Most cases of EBVaGC belonged to grade I. Conclusion: This research demonstrates a low prevalence of EBVaGC in Iran. Discrepancies in EBVaGC occurrence among countries could be attributed to epidemiological variables and dietary practices. A comprehensive studies will provide significant contributions to understanding of its etiology. [ABSTRACT FROM AUTHOR]

Published
2024

7. Prevalence of JC polyomavirus among rheumatoid arthritis and systemic lupus erythematosus patients and its correlation with vitamin D levels.

Author

Mokhtari, Golnaz Irani, Azaran, Azarakhsh, Rajahi, Elham, Hesam, Saeed, Ghahfarokhi, Azam Dehghani, and Makvandi, Manoochehr

Subjects
JOHN Cunningham virus, SYSTEMIC lupus erythematosus, VITAMIN D deficiency, DNA viruses, LUPUS erythematosus
Abstract

Background and Objectives: Vitamin D deficiency in viral infection associated with autoimmune diseases is well documented. This study assessed the prevalence of JC virus in patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), and its correlation with vitamin D level. Materials and Methods: Serum and urine samples were collected from 50 patients with RA and SLE. DNA was extracted and subjected to PCR test. Positive PCR products were sequenced, phylogenetic tree was constructed to determine the JC virus genotype. The patient's vitamin D level was evaluated. Results: Of 50 patients, 19 (38%) were diagnosed as RA, and 31 (62%) were identified as SLE. JC virus DNA was detected in 17 (34%) patients' urine samples including 5 (26.3%) RA and 12 (38.7%) SLE cases. JC virus DNA was detected 2 (4%) in patients' serum samples (one RA. and one SLE). JC virus genotype 3A was dominant. Interestingly, the SLE patients with positive JC virus showed lowered vitamin D compared to patients with negative JC virus (P<0.005). Conclusion: Given the high rate of JC virus, DNA detection and susceptibility of patients for PML development, it is recommended that detection of JC virus DNA and vitamin D level should be implemented for patients with RA/SLE prior to treatment. [ABSTRACT FROM AUTHOR]

Published
2024

34. Developing oncolytic Herpes simplex virus type 1 through UL39 knockout by CRISPR-Cas9

Author

Ebrahimi, Saeedeh, Makvandi, Manoochehr, Abbasi, Samaneh, Azadmanesh, Kayhan, and Teimoori, Ali

Subjects
crispr-cas9, viruses, lcsh:R, lcsh:Medicine, Original Article, herpes simplex virus type 1, ribonucleotide reductase, ul39, oncolytic virus
Abstract

Objective(s): Oncolytic Herpes simplex virus type 1 (HSV-1) has emerged as a promising strategy for cancer therapy. However, development of novel oncolytic mutants has remained a major challenge owing to low efficiency of conventional genome editing methods. Recently, CRISPR-Cas9 has revolutionized genome editing.Materials and Methods: In this study, we aimed to evaluate the capability of CRISPR-Cas9 to manipulate the UL39 gene to create oncolytic HSV-1. Herein, three sgRNAs were designed against the UL39 gene and transfected into HEK-293 cell line followed by infection with HSV-1 KOS.Results: After three rounds of plaque purification, several HSV-1 mutants were identified by PCR analysis and sequencing. One of these mutations in which 55 nucleotides were deleted resulted in a frameshift mutation that in turn produced a truncated protein with only 167 amino acids from 1137 amino acids. Functional analysis in Vero and primary fibroblast cells revealed that viral replication was significantly lower and plaque size was smaller in the HSV-1 mutant compared with HSV-1 KOS. Moreover, the relative amount of viral genome present in the supernatants of infected cells (Vero and primary fibroblast cells) with HSV-1 mutant was significantly decreased compared with those of HSV-1 KOS.Conclusion: Our data revealed that targeting UL39 with CRISPR-Cas9 could develop oncolytic HSV-1.

Published
2020

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