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Your search keyword '"Makker, S.P."' showing total 12 results
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12 results on '"Makker, S.P."'

1. Intravenous clusterin administration reduces myocardial infarct size in rats

Author

van Dijk, A., Vermond, R.A., Krijnen, P.A.J., Juffermans, L.J.M., Hahn, N.E., Makker, S.P., Aarden, L.A., Hack, C.E., Spreeuwenberg, M.D., van Rossum, A.C., Meischl, C., Paulus, W.J., van Milligen, F.J., Niessen, H.W.M., Pathology, Cardiology, Clinical chemistry, Epidemiology and Data Science, Physiology, and ICaR - Heartfailure and pulmonary arterial hypertension

Published
2010
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2. Mechanism-dependent selection of immunoglobulin gene library for obtaining covalent biocatalysis

Author

Reshetnyak, A.V., Armentano, M.F., Morse, H.C., Friboulet, A., Makker, S.P., Tramontano, A., Knorre, V.D., Gabibov, A.G., Ponomarenko, N.A., Laboratory of Biocatalysis, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry - RAS, Department of Pediatrics, School of Medicine, University of California [Davis] (UC Davis), University of California-University of California, Laboratory of Immunopathology - National Institute of Allergy and Infectious Diseases, National Institute of Health, Génie Enzymatique et Cellulaire (GEC), and Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)

Published
2007

3. Routes to covalent catalysis by reactive selection for nascent protein nucleophiles

Author

Reshetnyak, A.V., Armentano, M.F., Ponomarenko, N.A., Vizzuso, D., Durova, O.M., Ziganshin, R., Serebryakova, M., Govorun, V., Gololobov, G., Morse, H.C., Friboulet, A., Makker, S.P., Gabibov, A.G., Tramontano, A., Laboratory of Biocatalysis, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry - RAS, Department of Pediatrics, School of Medicine, University of California [Davis] (UC Davis), University of California-University of California, Laboratory of Immunopathology - National Institute of Allergy and Infectious Diseases, National Institute of Health, Génie Enzymatique et Cellulaire (GEC), and Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)

Abstract

International audience; Reactivity-based selection strategies have been used to enrich combinatorial libraries for encoded biocatalysts having revised substrate specificity or altered catalytic activity. This approach can also assist in artificial evolution of enzyme catalysis from protein templates without bias for predefined catalytic sites. The prevalence of covalent intermediates in enzymatic mechanisms suggests the universal utility of the covalent complex as the basis for selection. Covalent selection by phosphonate ester exchange was applied to a phage display library of antibody variable fragments (scFv) to sample the scope and mechanism of chemical reactivity in a naive molecular library. Selected scFv segregated into structurally related covalent and noncovalent binders. Clones that reacted covalently utilized tyrosine residues exclusively as the nucleophile. Two motifs were identified by structural analysis, recruiting distinct Tyr residues of the light chain. Most clones employed Tyr32 in CDR-L1, whereas a unique clone (A.17) reacted at Tyr36 in FR-L2. Enhanced phosphonylation kinetics and modest amidase activity of A.17 suggested a primitive catalytic site. Covalent selection may thus provide access to protein molecules that approximate an early apparatus for covalent catalysis.

Published
2007

7. Enhancement of in situ immune complex formation in isolated perfused kidneys in Heymann's nephritis.

Author

Makker, S.P., Kirson, I.J., and Moorthy, B.

Subjects
*KIDNEY diseases, *IMMUNE complexes, *ANTIGENS, *IMMUNOGLOBULINS, *CHROMATOGRAPHIC analysis, *IMMUNOELECTROPHORESIS
Abstract

A preparation of the putative antibody of HN (Heymann's nephritis) lacking preformed immune complexes, obtained from the acid eluates of kidneys with HN, purified through ion exchange and gel-filtration chromatography, characterized by immunoelectrophoresis, sucrose density-gradient ultracentrifugation, 125I-Clq assay for immune complexes, and specific for the brush border of rat proximal renal tubule was injected intravenously, intra-arterially directly into the left renal artery of a kidney with intact normal circulation, and into the isolated butter-perfused kidney, respectively, in three groups of rats. Controls were injected with non-antibody IgG, and antibody absorbed with the specific antigen. Localization of antibody was studied by direct immunofluorescence. Prompt localization of antibody and formation of in situ immune complexes occurred in the isolated perfused kidney. No localization of antibody was seen following the intrarenal arterial injection in the unperfused kidney with intact circulation, and a delay of 5 days was noted for the localization to develop upon intravenous injection. These results show that, in the homologous antibody-induced HN, the preparation of isolated perfused kidney considerably enhances the formation of in situ immune complexes in glomeruli as compared to the unperfused kidney with intact circulation in which the antibody is injected either directly into the renal artery or systemically through a peripheral vein. [ABSTRACT FROM AUTHOR]

Published
1982

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