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Biophysical variables play a crucial role in understanding phenological stages and crop dynamics, optimizing ultimate agricultural practices, and achieving sustainable crop yields. This study examined the effectiveness of the Sentinel-2 Biophysical Processor (S2BP) in accurately estimating crop dynamics descriptors, including fractional vegetation cover (FVC), leaf area index (LAI), leaf chlorophyll a and b (LCab), and canopy water content (CWC). The evaluation was conducted using estimation quality indicators (EQIs) and comprehensive ground throughout the entire growing season at the field scale. To identify soil and vegetation pixels, the spectral unmixing technique was employed. According to the EQIs, the best retrievals were obtained for FVC in around 99.9% of the 23,976 pixels that were analyzed during the growth season. For LAI, LCab, and CWC, over 60% of the examined pixels had inputs that were out-of-range. Furthermore, in over 35% of the pixels, the output values for LCab and CWC were out-of-range. The FVC, LAI, and LCab estimates agreed well with ground measurements (R2 = 0.62–0.85), whereas a discrepancy was observed for CWC estimates when compared with ground measurements (R2 = 0.51). Furthermore, the uncertainties of FVC, LAI, LCab, and CWC estimates were 0.09, 0.81 m2/m2, 60.85 µg/cm2, and 0.02 g/cm2 through comparisons to ground FVC, LAI, Cab, and CWC measurements, respectively. Considering EQIs and uncertainty metrics, the order of the estimation accuracy of the four variables was FVC > LAI > LCab > CWC. Our analysis revealed that temporal variations of FVC, LAI, and LCab were primarily driven by field-scale events like sowing date, growing period, and harvesting time, highlighting their sensitivity to agricultural practices. The robustness of S2BP results could be enhanced by implementing a pixel identification algorithm, like embedding spectral unmixing. Overall, this study provides detailed, pixel-by-pixel insights into the performance of S2BP in estimating FVC, LAI, LCab, and CWC, which are crucial for monitoring crop dynamics in precision agriculture. [ABSTRACT FROM AUTHOR]