659 results on '"Maiuri P"'
Search Results
2. The importance of considering competing risks in recurrence analysis of intracranial meningioma.
- Author
-
Mirian, Christian, Jensen, Lasse, Juratli, Tareq, Maier, Andrea, Torp, Sverre, Shih, Helen, Morshed, Ramin, Young, Jacob, Magill, Stephen, Bertero, Luca, Stummer, Walter, Spille, Dorothee, Brokinkel, Benjamin, Oya, Soichi, Miyawaki, Satoru, Saito, Nobuhito, Proescholdt, Martin, Kuroi, Yasuhiro, Gousias, Konstantinos, Simon, Matthias, Moliterno, Jennifer, Prat-Acin, Ricardo, Goutagny, Stéphane, Prabhu, Vikram, Tsiang, John, Wach, Johannes, Güresir, Erdem, Yamamoto, Junkoh, Kim, Young, Lee, Joo, Koshy, Matthew, Perumal, Karthikeyan, Baskaya, Mustafa, Cannon, Donald, Shrieve, Dennis, Suh, Chang-Ok, Chang, Jong, Kamenova, Maria, Straumann, Sven, Soleman, Jehuda, Eyüpoglu, Ilker, Catalan, Tony, Lui, Austin, Wang, Fang, Guo, Fuyou, Góes, Pedro, de Paiva Neto, Manoel, Jamshidi, Aria, Komotar, Ricardo, Ivan, Michael, Luther, Evan, Souhami, Luis, Guiot, Marie-Christine, Csonka, Tamás, Endo, Toshiki, Barrett, Olivia, Jensen, Randy, Gupta, Tejpal, Patel, Akash, Klisch, Tiemo, Kim, Jun, Maiuri, Francesco, Barresi, Valeria, Tabernero, María, Skyrman, Simon, Broechner, Anders, Bach, Mathias, Law, Ian, Scheie, David, Kristensen, Bjarne, Munch, Tina, Meling, Torstein, Fugleholm, Kåre, Blanche, Paul, Mathiesen, Tiit, McDermott, Mike, and Theodosopoulos, Philip
- Subjects
Competing risk ,Meningioma ,Neuro-oncology ,Recurrence ,Humans ,Aged ,Meningioma ,Meningeal Neoplasms ,Neoplasm Recurrence ,Local ,Retrospective Studies ,Risk Assessment - Abstract
BACKGROUND: The risk of recurrence is overestimated by the Kaplan-Meier method when competing events, such as death without recurrence, are present. Such overestimation can be avoided by using the Aalen-Johansen method, which is a direct extension of Kaplan-Meier that accounts for competing events. Meningiomas commonly occur in older individuals and have slow-growing properties, thereby warranting competing risk analysis. The extent to which competing events are considered in meningioma literature is unknown, and the consequences of using incorrect methodologies in meningioma recurrence risk analysis have not been investigated. METHODS: We surveyed articles indexed on PubMed since 2020 to assess the usage of competing risk analysis in recent meningioma literature. To compare recurrence risk estimates obtained through Kaplan-Meier and Aalen-Johansen methods, we applied our international database comprising ~ 8,000 patients with a primary meningioma collected from 42 institutions. RESULTS: Of 513 articles, 169 were eligible for full-text screening. There were 6,537 eligible cases from our PERNS database. The discrepancy between the results obtained by Kaplan-Meier and Aalen-Johansen was negligible among low-grade lesions and younger individuals. The discrepancy increased substantially in the patient groups associated with higher rates of competing events (older patients with high-grade lesions). CONCLUSION: The importance of considering competing events in recurrence risk analysis is poorly recognized as only 6% of the studies we surveyed employed Aalen-Johansen analyses. Consequently, most of the previous literature has overestimated the risk of recurrence. The overestimation was negligible for studies involving low-grade lesions in younger individuals; however, overestimation might have been substantial for studies on high-grade lesions.
- Published
- 2024
3. Giant ultrafast dichroism and birefringence with active nonlocal metasurfaces
- Author
-
Giulia Crotti, Mert Akturk, Andrea Schirato, Vincent Vinel, Anton A. Trifonov, Ivan C. Buchvarov, Dragomir N. Neshev, Remo Proietti Zaccaria, Paolo Laporta, Aristide Lemaître, Giuseppe Leo, Giulio Cerullo, Margherita Maiuri, and Giuseppe Della Valle
- Subjects
Applied optics. Photonics ,TA1501-1820 ,Optics. Light ,QC350-467 - Abstract
Abstract Switching of light polarization on the sub-picosecond timescale is a crucial functionality for applications in a variety of contexts, including telecommunications, biology and chemistry. The ability to control polarization at ultrafast speed would pave the way for the development of unprecedented free-space optical links and of novel techniques for probing dynamical processes in complex systems, as chiral molecules. Such high switching speeds can only be reached with an all-optical paradigm, i.e., engineering active platforms capable of controlling light polarization via ultrashort laser pulses. Here we demonstrate giant modulation of dichroism and birefringence in an all-dielectric metasurface, achieved at low fluences of the optical control beam. This performance, which leverages the many degrees of freedom offered by all-dielectric active metasurfaces, is obtained by combining a high-quality factor nonlocal resonance with the giant third-order optical nonlinearity dictated by photogenerated hot carriers at the semiconductor band edge.
- Published
- 2024
- Full Text
- View/download PDF
4. Preclinical models of breast cancer: B6BC, a transplantable hormone receptor-positive C57BL/6 mouse cell line
- Author
-
Perez-Lanzon, Maria, Maiuri, Maria Chiara, Lopez-Otin, Carlos, and Kroemer, Guido
- Published
- 2024
- Full Text
- View/download PDF
5. Giant ultrafast dichroism and birefringence with active nonlocal metasurfaces
- Author
-
Crotti, Giulia, Akturk, Mert, Schirato, Andrea, Vinel, Vincent, Trifonov, Anton A., Buchvarov, Ivan C., Neshev, Dragomir N., Proietti Zaccaria, Remo, Laporta, Paolo, Lemaître, Aristide, Leo, Giuseppe, Cerullo, Giulio, Maiuri, Margherita, and Della Valle, Giuseppe
- Published
- 2024
- Full Text
- View/download PDF
6. Letter to the editor regarding “Predictive factors of the postoperative proptosis recovery in surgery of spheno-orbital meningiomas”
- Author
-
Corvino, Sergio and Maiuri, Francesco
- Published
- 2024
- Full Text
- View/download PDF
7. N2FXm, a method for joint nuclear and cytoplasmic volume measurements, unravels the osmo-mechanical regulation of nuclear volume in mammalian cells
- Author
-
Pennacchio, Fabrizio A., Poli, Alessandro, Pramotton, Francesca Michela, Lavore, Stefania, Rancati, Ilaria, Cinquanta, Mario, Vorselen, Daan, Prina, Elisabetta, Romano, Orso Maria, Ferrari, Aldo, Piel, Matthieu, Cosentino Lagomarsino, Marco, and Maiuri, Paolo
- Published
- 2024
- Full Text
- View/download PDF
8. Dynamic changes in LINC00458/HBL1 lncRNA expression during hiPSC differentiation to cardiomyocytes
- Author
-
Maciak, Patrycja, Suder, Agnieszka, Wadas, Jakub, Aronimo, Faith, Maiuri, Paolo, Bochenek, Michał, Pyrc, Krzysztof, Kula-Pacurar, Anna, and Pabis, Marta
- Published
- 2024
- Full Text
- View/download PDF
9. BIN1 regulates actin-membrane interactions during IRSp53-dependent filopodia formation
- Author
-
Laura Picas, Charlotte André-Arpin, Franck Comunale, Hugo Bousquet, Feng-Ching Tsai, Félix Rico, Paolo Maiuri, Julien Pernier, Stéphane Bodin, Anne-Sophie Nicot, Jocelyn Laporte, Patricia Bassereau, Bruno Goud, Cécile Gauthier-Rouvière, and Stéphanie Miserey
- Subjects
Biology (General) ,QH301-705.5 - Abstract
Abstract Amphiphysin 2 (BIN1) is a membrane and actin remodeling protein mutated in congenital and adult centronuclear myopathies. Here, we report an unexpected function of this N-BAR domain protein BIN1 in filopodia formation. We demonstrated that BIN1 expression is necessary and sufficient to induce filopodia formation. BIN1 is present at the base of forming filopodia and all along filopodia, where it colocalizes with F-actin. We identify that BIN1-mediated filopodia formation requires IRSp53, which allows its localization at negatively-curved membrane topologies. Our results show that BIN1 bundles actin in vitro. Finally, we identify that BIN1 regulates the membrane-to-cortex architecture and functions as a molecular platform to recruit actin-binding proteins, dynamin and ezrin, to promote filopodia formation.
- Published
- 2024
- Full Text
- View/download PDF
10. Inhibition of acyl-CoA binding protein (ACBP) by means of a GABAARγ2-derived peptide
- Author
-
Gerasimos Anagnostopoulos, Ester Saavedra, Flavia Lambertucci, Omar Motiño, Jordan Dimitrov, David Roiz-Valle, Victor Quesada, Karla Alvarez-Valadez, Hui Chen, Allan Sauvat, Yan Rong, Uxía Nogueira-Recalde, Sijing Li, Léa Montégut, Mojgan Djavaheri-Mergny, Maria Castedo, Carlos Lopez-Otin, Maria Chiara Maiuri, Isabelle Martins, and Guido Kroemer
- Subjects
Cytology ,QH573-671 - Abstract
Abstract Acyl-CoA binding protein (ACBP) encoded by diazepam binding inhibitor (DBI) is an extracellular inhibitor of autophagy acting on the gamma-aminobutyric acid A receptor (GABAAR) γ2 subunit (GABAARγ2). Here, we show that lipoanabolic diets cause an upregulation of GABAARγ2 protein in liver hepatocytes but not in other major organs. ACBP/DBI inhibition by systemically injected antibodies has been demonstrated to mediate anorexigenic and organ-protective, autophagy-dependent effects. Here, we set out to develop a new strategy for developing ACBP/DBI antagonists. For this, we built a molecular model of the interaction of ACBP/DBI with peptides derived from GABAARγ2. We then validated the interaction between recombinant and native ACBP/DBI protein and a GABAARγ2-derived eicosapeptide (but not its F77I mutant) by pull down experiments or surface plasmon resonance. The GABAARγ2-derived eicosapeptide inhibited the metabolic activation of hepatocytes by recombinant ACBP/DBI protein in vitro. Moreover, the GABAARγ2-derived eicosapeptide (but not its F77I-mutated control) blocked appetite stimulation by recombinant ACBP/DBI in vivo, induced autophagy in the liver, and protected mice against the hepatotoxin concanavalin A. We conclude that peptidomimetics disrupting the interaction between ACBP/DBI and GABAARγ2 might be used as ACBP/DBI antagonists. This strategy might lead to the future development of clinically relevant small molecules of the ACBP/DBI system.
- Published
- 2024
- Full Text
- View/download PDF
11. Inhibition of acyl-CoA binding protein (ACBP) by means of a GABAARγ2-derived peptide
- Author
-
Anagnostopoulos, Gerasimos, Saavedra, Ester, Lambertucci, Flavia, Motiño, Omar, Dimitrov, Jordan, Roiz-Valle, David, Quesada, Victor, Alvarez-Valadez, Karla, Chen, Hui, Sauvat, Allan, Rong, Yan, Nogueira-Recalde, Uxía, Li, Sijing, Montégut, Léa, Djavaheri-Mergny, Mojgan, Castedo, Maria, Lopez-Otin, Carlos, Maiuri, Maria Chiara, Martins, Isabelle, and Kroemer, Guido
- Published
- 2024
- Full Text
- View/download PDF
12. Collective rotational motion of freely-expanding T84 epithelial cell colonies
- Author
-
Ascione, Flora, Caserta, Sergio, Esposito, Speranza, Villella, Valeria Rachela, Maiuri, Luigi, Nejad, Mehrana R., Doostmohammadi, Amin, Yeomans, Julia M., and Guido, Stefano
- Subjects
Condensed Matter - Soft Condensed Matter - Abstract
Coordinated rotational motion is an intriguing, yet still elusive mode of collective cell migration, which is relevant in pathological and morphogenetic processes. Most of the studies on this topic have been carried out on confined epithelial cells. The driver of collective rotation in such conditions has not been clearly elucidated, although it has been speculated that spatial confinement can play an essential role in triggering cell rotation. Here, we study the growth of epithelial cell colonies freely expanding (i.e., with no physical constraints) on the surface of cell culture plates, a case which has received scarce attention in the literature. We find that coordinated cell rotation spontaneously occurs in cell clusters in the free growth regime, thus implying that cell confinement is not necessary to elicit collective rotation as previously suggested. The collective rotation was size and shape dependent: a highly coordinated disk-like rotation was found in small cell clusters with a round shape, while collective rotation was suppressed in large irregular cell clusters generated by merging of different clusters in the course of their growth. The angular motion was persistent in the same direction, although clockwise and anticlockwise rotations were equally likely to occur among different cell clusters. Radial cell velocity was low as compared to the angular velocity. A clear difference in morphology was observed between cells at the periphery and the ones in the core of the clusters, the former being more elongated and spread out as compared to the latter. Overall, our results provide the first quantitative and systematic evidence that coordinated cell rotation does not require a spatial confinement and occurs spontaneously in freely expanding epithelial cell colonies.
- Published
- 2022
- Full Text
- View/download PDF
13. Vibration isolation could boost performance of near-infrared organic LEDs
- Author
-
Maiuri, Margherita
- Published
- 2024
- Full Text
- View/download PDF
14. N2FXm, a method for joint nuclear and cytoplasmic volume measurements, unravels the osmo-mechanical regulation of nuclear volume in mammalian cells
- Author
-
Fabrizio A. Pennacchio, Alessandro Poli, Francesca Michela Pramotton, Stefania Lavore, Ilaria Rancati, Mario Cinquanta, Daan Vorselen, Elisabetta Prina, Orso Maria Romano, Aldo Ferrari, Matthieu Piel, Marco Cosentino Lagomarsino, and Paolo Maiuri
- Subjects
Science - Abstract
Abstract In eukaryotes, cytoplasmic and nuclear volumes are tightly regulated to ensure proper cell homeostasis. However, current methods to measure cytoplasmic and nuclear volumes, including confocal 3D reconstruction, have limitations, such as relying on two-dimensional projections or poor vertical resolution. Here, to overcome these limitations, we describe a method, N2FXm, to jointly measure cytoplasmic and nuclear volumes in single cultured adhering human cells, in real time, and across cell cycles. We find that this method accurately provides joint size over dynamic measurements and at different time resolutions. Moreover, by combining several experimental perturbations and analyzing a mathematical model including osmotic effects and tension, we show that N2FXm can give relevant insights on how mechanical forces exerted by the cytoskeleton on the nuclear envelope can affect the growth of nucleus volume by biasing nuclear import. Our method, by allowing for accurate joint nuclear and cytoplasmic volume dynamic measurements at different time resolutions, highlights the non-constancy of the nucleus/cytoplasm ratio along the cell cycle.
- Published
- 2024
- Full Text
- View/download PDF
15. Dynamic changes in LINC00458/HBL1 lncRNA expression during hiPSC differentiation to cardiomyocytes
- Author
-
Patrycja Maciak, Agnieszka Suder, Jakub Wadas, Faith Aronimo, Paolo Maiuri, Michał Bochenek, Krzysztof Pyrc, Anna Kula-Pacurar, and Marta Pabis
- Subjects
Medicine ,Science - Abstract
Abstract Long non-coding RNAs (lncRNAs) constitute the largest and most diverse class of non-coding RNAs. They localize to the nucleus, cytoplasm, or both compartments, and regulate gene expression through various mechanisms at multiple levels. LncRNAs tend to evolve faster and present higher tissue- and developmental stage-specific expression than protein-coding genes. Initially considered byproducts of erroneous transcription without biological function, lncRNAs are now recognized for their involvement in numerous biological processes, such as immune response, apoptosis, pluripotency, reprogramming, and differentiation. In this study, we focused on Heart Brake lncRNA 1 (HBL1), a lncRNA recently reported to modulate the process of pluripotent stem cell differentiation toward cardiomyocytes. We employed RT-qPCR and high-resolution RNA FISH to monitor the expression and localization of HBL1 during the differentiation progression. Our findings indicate a significant increase in HBL1 expression during mesodermal and cardiac mesodermal stages, preceding an anticipated decrease in differentiated cells. We detected the RNA in discrete foci in both the nucleus and in the cytoplasm. In the latter compartment, we observed colocalization of HBL1 with Y-box binding protein 1 (YB-1), which likely results from an interaction between the RNA and the protein, as the two were found to be coimmunoprecipitated in RNP-IP experiments. Finally, we provide evidence that HBL1, initially reported as an independent lncRNA gene, is part of the LINC00458 (also known as lncRNA-ES3 or ES3) gene, forming the last exon of some LINC00458 splice isoforms.
- Published
- 2024
- Full Text
- View/download PDF
16. Methods and computational tools to study eukaryotic cell migration in vitro
- Author
-
Elvira Toscano, Elena Cimmino, Fabrizio A. Pennacchio, Patrizia Riccio, Alessandro Poli, Yan-Jun Liu, Paolo Maiuri, Leandra Sepe, and Giovanni Paolella
- Subjects
cell migration ,cell tracking ,trajectory analysis ,chemotaxis ,durotaxis ,Biology (General) ,QH301-705.5 - Abstract
Cellular movement is essential for many vital biological functions where it plays a pivotal role both at the single cell level, such as during division or differentiation, and at the macroscopic level within tissues, where coordinated migration is crucial for proper morphogenesis. It also has an impact on various pathological processes, one for all, cancer spreading. Cell migration is a complex phenomenon and diverse experimental methods have been developed aimed at dissecting and analysing its distinct facets independently. In parallel, corresponding analytical procedures and tools have been devised to gain deep insight and interpret experimental results. Here we review established experimental techniques designed to investigate specific aspects of cell migration and present a broad collection of historical as well as cutting-edge computational tools used in quantitative analysis of cell motion.
- Published
- 2024
- Full Text
- View/download PDF
17. All-optical Reconfiguration of Ultrafast Dichroism in Gold Metasurfaces
- Author
-
Schirato, Andrea, Toma, Andrea, Zaccaria, Remo Proietti, Alabastri, Alessandro, Cerullo, Giulio, Della Valle, Giuseppe, and Maiuri, Margherita
- Subjects
Physics - Optics - Abstract
Optical metasurfaces have come into the spotlight as a promising platform for light manipulation at the nanoscale, including ultrafast all-optical control via excitation with femtosecond laser pulses. Recently, dichroic metasurfaces have been exploited to modulate the polarization state of light with unprecedented speed. Here, we theoretically predict and experimentally demonstrate by pump-probe spectroscopy the capability to reconfigure the ultrafast dichroic signal of a gold metasurface by simply acting on the polarization of the pump pulse, which is shown to reshape the spatio-temporal distribution of the optical perturbation. The photoinduced anisotropic response, driven by out-of-equilibrium carriers and extinguished in a sub-picosecond temporal window, is readily controlled in intensity by tuning the polarization direction of the excitation up to a full sign reversal. This work proves that nonlinear metasurfaces offer the flexibility to tailor their ultrafast optical response in a fully all-optically reconfigurable platform.
- Published
- 2022
18. Correction: Preclinical models of breast cancer: B6BC, a transplantable hormone receptor-positive C57BL/6 mouse cell line
- Author
-
Perez-Lanzon, Maria, Maiuri, Maria Chiara, Lopez-Otin, Carlos, and Kroemer, Guido
- Published
- 2024
- Full Text
- View/download PDF
19. PIP4K2B is mechanoresponsive and controls heterochromatin-driven nuclear softening through UHRF1
- Author
-
Poli, Alessandro, Pennacchio, Fabrizio A., Ghisleni, Andrea, di Gennaro, Mariagrazia, Lecacheur, Margaux, Nastały, Paulina, Crestani, Michele, Pramotton, Francesca M., Iannelli, Fabio, Beznusenko, Galina, Mironov, Alexander A., Panzetta, Valeria, Fusco, Sabato, Sheth, Bhavwanti, Poulikakos, Dimos, Ferrari, Aldo, Gauthier, Nils, Netti, Paolo A., Divecha, Nullin, and Maiuri, Paolo
- Published
- 2023
- Full Text
- View/download PDF
20. Meningiomas of the rolandic region: risk factors for motor deficit and role of intra-operative monitoring
- Author
-
Maiuri, Francesco and Corvino, Sergio
- Published
- 2023
- Full Text
- View/download PDF
21. Tissue fluidification promotes a cGAS–STING cytosolic DNA response in invasive breast cancer
- Author
-
Frittoli, Emanuela, Palamidessi, Andrea, Iannelli, Fabio, Zanardi, Federica, Villa, Stefano, Barzaghi, Leonardo, Abdo, Hind, Cancila, Valeria, Beznoussenko, Galina V., Della Chiara, Giulia, Pagani, Massimiliano, Malinverno, Chiara, Bhattacharya, Dipanjan, Pisati, Federica, Yu, Weimiao, Galimberti, Viviana, Bonizzi, Giuseppina, Martini, Emanuele, Mironov, Alexander A., Gioia, Ubaldo, Ascione, Flora, Li, Qingsen, Havas, Kristina, Magni, Serena, Lavagnino, Zeno, Pennacchio, Fabrizio Andrea, Maiuri, Paolo, Caponi, Silvia, Mattarelli, Maurizio, Martino, Sabata, d’Adda di Fagagna, Fabrizio, Rossi, Chiara, Lucioni, Marco, Tancredi, Richard, Pedrazzoli, Paolo, Vecchione, Andrea, Petrini, Cristiano, Ferrari, Francesco, Lanzuolo, Chiara, Bertalot, Giovanni, Nader, Guilherme, Foiani, Marco, Piel, Matthieu, Cerbino, Roberto, Giavazzi, Fabio, Tripodo, Claudio, and Scita, Giorgio
- Published
- 2023
- Full Text
- View/download PDF
22. In Silico Ultrafast Nonlinear Spectroscopy Meets Experiments: The Case of Perylene Bisimide Dye
- Author
-
Segatta, Francesco, Russo, Mattia, Nascimento, Daniel R, Presti, Davide, Rigodanza, Francesco, Nenov, Artur, Bonvicini, Andrea, Arcioni, Alberto, Mukamel, Shaul, Maiuri, Margherita, Muccioli, Luca, Govind, Niranjan, Cerullo, Giulio, and Garavelli, Marco
- Subjects
Bioengineering ,Theoretical and Computational Chemistry ,Biochemistry and Cell Biology ,Computer Software ,Chemical Physics - Abstract
Spectroscopy simulations are of paramount importance for the interpretation of experimental electronic spectra, the disentangling of overlapping spectral features, and the tracing of the microscopic origin of the observed signals. Linear and nonlinear simulations are based on the results drawn from electronic structure calculations that provide the necessary parameterization of the molecular systems probed by light. Here, we investigate the applicability of excited-state properties obtained from linear-response time-dependent density functional theory (TDDFT) in the description of nonlinear spectra by employing the pseudowavefunction approach and compare them with benchmarks from highly accurate RASSCF/RASPT2 calculations and with high temporal resolution experimental results. As a test case, we consider the prediction of femtosecond transient absorption and two-dimensional electronic spectroscopy of a perylene bisimide dye in solution. We find that experimental signals are well reproduced by both theoretical approaches, showing that the computationally cheaper TDDFT can be a suitable option for the simulation of nonlinear spectroscopy of molecular systems that are too large to be treated with higher-level RASSCF/RASPT2 methods.
- Published
- 2021
23. Autoimmunity affecting the biliary tract fuels the immunosurveillance of cholangiocarcinoma
- Author
-
Paillet, Juliette, Plantureux, Céleste, Lévesque, Sarah, Le Naour, Julie, Stoll, Gautier, Sauvat, Allan, Caudana, Pamela, Boari, Jimena Tosello, Bloy, Norma, Lachkar, Sylvie, Martins, Isabelle, Opolon, Paule, Checcoli, Andrea, Delaune, Agathe, Robil, Noémie, de la Grange, Pierre, Hamroune, Juliette, Letourneur, Franck, Autret, Gwennhael, Leung, Patrick SC, Gershwin, M Eric, Zhu, Jie S, Kurth, Mark J, Lekbaby, Bouchra, Augustin, Jérémy, Kim, Youra, Gujar, Shashi, Coulouarn, Cédric, Fouassier, Laura, Zitvogel, Laurence, Piaggio, Eliane, Housset, Chantal, Soussan, Patrick, Maiuri, Maria Chiara, Kroemer, Guido, and Pol, Jonathan G
- Subjects
Rare Diseases ,Autoimmune Disease ,Liver Disease ,Chronic Liver Disease and Cirrhosis ,Digestive Diseases ,Biotechnology ,Cancer ,Digestive Diseases - (Gallbladder) ,Liver Cancer ,Aetiology ,2.1 Biological and endogenous factors ,Animals ,Autoimmunity ,Bile Duct Neoplasms ,CD4-Positive T-Lymphocytes ,CD8-Positive T-Lymphocytes ,Cell Line ,Tumor ,Cholangiocarcinoma ,Cholangitis ,Cytokines ,Female ,Forkhead Transcription Factors ,Liver ,Mice ,Inbred C57BL ,Monitoring ,Immunologic ,Neoplasms ,Experimental ,Medical and Health Sciences ,Immunology - Abstract
Cholangiocarcinoma (CCA) results from the malignant transformation of cholangiocytes. Primary sclerosing cholangitis (PSC) and primary biliary cholangitis (PBC) are chronic diseases in which cholangiocytes are primarily damaged. Although PSC is an inflammatory condition predisposing to CCA, CCA is almost never found in the autoimmune context of PBC. Here, we hypothesized that PBC might favor CCA immunosurveillance. In preclinical murine models of cholangitis challenged with syngeneic CCA, PBC (but not PSC) reduced the frequency of CCA development and delayed tumor growth kinetics. This PBC-related effect appeared specific to CCA as it was not observed against other cancers, including hepatocellular carcinoma. The protective effect of PBC was relying on type 1 and type 2 T cell responses and, to a lesser extent, on B cells. Single-cell TCR/RNA sequencing revealed the existence of TCR clonotypes shared between the liver and CCA tumor of a PBC host. Altogether, these results evidence a mechanistic overlapping between autoimmunity and cancer immunosurveillance in the biliary tract.
- Published
- 2021
24. Topographic Patterns of Intracranial Meningioma Recurrences—Systematic Review with Clinical Implication
- Author
-
Sergio Corvino, Roberto Altieri, Giuseppe La Rocca, Amedeo Piazza, Giuseppe Corazzelli, Carmela Palmiero, Giuseppe Mariniello, Francesco Maiuri, Andrea Elefante, and Oreste de Divitiis
- Subjects
intracranial meningiomas ,recurrences ,topographic pattern ,classification ,risk factors ,radiation therapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: While several risk factors for recurrences have been defined, the topographic pattern of meningioma recurrences after surgical resection has been scarcely investigated. The possibility of theoretically predicting the site of recurrence not only allows us to better understand the pathogenetic bases of the disease and consequently to drive the development of new targeted therapies, but also guides the decision-making process for treatment strategies and tailored follow-ups to decrease/prevent recurrence. Methods: The authors performed a comprehensive and detailed systematic literature review of the EMBASE and MEDLINE electronic online databases regarding the topographic pattern of recurrence after surgical treatment for intracranial meningiomas. Demographics and histopathological, neuroradiological and treatment data, pertinent to the topography of recurrences, as well as time to recurrences, were extracted and analyzed. Results: Four studies, including 164 cases of recurrences according to the inclusion criteria, were identified. All studies consider the possibility of recurrence at the previous dural site; three out of four, which are the most recent, consider 1 cm outside the previous dural margin to be the main limit to distinguish recurrences closer to the previous site from those more distant. Recurrences mainly occur within or close to the surgical bed; higher values of proliferation index are associated with recurrences close to the original site rather than within it. Conclusions: Further studies, including genomic characterization of different patterns of recurrence, will better clarify the main features affecting the topography of recurrences. A comparison between topographic classifications of intracranial meningioma recurrences after surgery and after radiation treatment could provide further interesting information.
- Published
- 2024
- Full Text
- View/download PDF
25. Transient optical symmetry breaking for ultrafast broadband dichroism in plasmonic metasurfaces
- Author
-
Schirato, Andrea, Maiuri, Margherita, Toma, Andrea, Fugattini, Silvio, Zaccaria, Remo Proietti, Laporta, Paolo, Nordlander, Peter, Cerullo, Giulio, Alabastri, Alessandro, and Della Valle, Giuseppe
- Subjects
Physics - Optics - Abstract
Ultrafast nanophotonics is an emerging research field aimed at the development of nanodevices capable of light modulation with unprecedented speed. A promising approach exploits the optical nonlinearity of nanostructured materials (either metallic or dielectric) to modulate their effective permittivity via interaction with intense ultrashort laser pulses. While the ultrafast temporal dynamics of such nanostructures following photoexcitation has been studied in depth, sub-ps transient spatial inhomogeneities taking place at the nanoscale have been so far almost ignored. Here we theoretically predict and experimentally demonstrate that the inhomogeneous space-time distribution of photogenerated hot carriers induces a transient symmetry breaking in a plasmonic metasurface made of highly symmetric metaatoms. The process is fully reversible, and results in a broadband transient dichroic optical response with a recovery of the initial isotropic state in less than 1 picosecond, overcoming the speed bottleneck caused by slower relaxation processes, such as electron-phonon and phonon-phonon scattering. Our results pave the way to the development of ultrafast dichroic devices, capable of Tera bit/s modulation of light polarization.
- Published
- 2020
- Full Text
- View/download PDF
26. Mammary carcinoma: toward a realistic mouse model of incurable cancers
- Author
-
Maria Pérez-Lanzón, Maria Chiara Maiuri, Carlos Lopez-Otin, and Guido Kroemer
- Subjects
Immunologic diseases. Allergy ,RC581-607 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
ABSTRACTAs long as breast cancer (BC) stays under immunosurveillance, it can be controlled by treatments eliciting anticancer immune responses. However, once BC escapes immunosurveillance, it becomes therapeutically uncontrollable. A paper in the Journal for ImmunoTherapy of Cancer describes a new hormone receptor-positive BC cell line generating incurable tumors in C57BL/6 mice.
- Published
- 2023
- Full Text
- View/download PDF
27. Strongly coupled coherent phonons in single-layer MoS$_2$
- Author
-
Trovatello, C., Miranda, H. P. C., Molina-Sánchez, A., Varillas, R. Borrego, Manzoni, C., Moretti, L., Ganzer, L., Maiuri, M., Wang, J., Dumcenco, D., Kis, A., Wirtz, L., Marini, A., Soavi, G., Ferrari, A. C., Cerullo, G., Sangalli, D., and Conte, S. Dal
- Subjects
Condensed Matter - Materials Science ,Condensed Matter - Mesoscale and Nanoscale Physics ,Physics - Applied Physics - Abstract
We present a transient absorption setup combining broadband detection over the visible-UV range with high temporal resolution ($\sim$20fs) which is ideally suited to trigger and detect vibrational coherences in different classes of materials. We generate and detect coherent phonons (CPs) in single layer (1L) MoS$_2$, as a representative semiconducting 1L-transition metal dichalcogenide (TMD), where the confined dynamical interaction between excitons and phonons is unexplored. The coherent oscillatory motion of the out-of-plane $A'_{1}$ phonons, triggered by the ultrashort laser pulses, dynamically modulates the excitonic resonances on a timescale of few tens fs. We observe an enhancement by almost two orders of magnitude of the CP amplitude when detected in resonance with the C exciton peak, combined with a resonant enhancement of CP generation efficiency. Ab initio calculations of the change in 1L-MoS$_2$ band structure induced by the $A'_{1}$ phonon displacement confirm a strong coupling with the C exciton. The resonant behavior of the CP amplitude follows the same spectral profile of the calculated Raman susceptibility tensor. This demonstrates that CP excitation in 1L-MoS$_2$ can be described as a Raman-like scattering process. These results explain the CP generation process in 1L-TMDs, paving the way for coherent all-optical control of excitons in layered materials in the THz frequency range.
- Published
- 2019
- Full Text
- View/download PDF
28. PIP4K2B is mechanoresponsive and controls heterochromatin-driven nuclear softening through UHRF1
- Author
-
Alessandro Poli, Fabrizio A. Pennacchio, Andrea Ghisleni, Mariagrazia di Gennaro, Margaux Lecacheur, Paulina Nastały, Michele Crestani, Francesca M. Pramotton, Fabio Iannelli, Galina Beznusenko, Alexander A. Mironov, Valeria Panzetta, Sabato Fusco, Bhavwanti Sheth, Dimos Poulikakos, Aldo Ferrari, Nils Gauthier, Paolo A. Netti, Nullin Divecha, and Paolo Maiuri
- Subjects
Science - Abstract
PIP4Ks are phosphoinositide kinases often dysregulated in cancer. Here Poli and colleagues find that PIP4K2B is downregulated on soft substrates, and its depletion leads to altered nuclear mechanical properties and defects in cell spreading and motility.
- Published
- 2023
- Full Text
- View/download PDF
29. Ultrafast hot electron dynamics in plasmonic nanostructures: experiments, modelling, design
- Author
-
Schirato Andrea, Maiuri Margherita, Cerullo Giulio, and Della Valle Giuseppe
- Subjects
electron–phonon coupling ,hot electrons ,localized surface plasmon resonances ,metal nanoparticles ,ultrafast spectroscopy ,Physics ,QC1-999 - Abstract
Metallic nanostructures exhibit localized surface plasmons (LSPs), which offer unprecedented opportunities for advanced photonic materials and devices. Following resonant photoexcitation, LSPs quickly dephase, giving rise to a distribution of energetic ‘hot’ electrons in the metal. These out-of-equilibrium carriers undergo ultrafast internal relaxation processes, nowadays pivotal in a variety of applications, from photodetection and sensing to the driving of photochemical reactions and ultrafast all-optical modulation of light. Despite the intense research activity, exploitation of hot carriers for real-world nanophotonic devices remains extremely challenging. This is due to the complexity inherent to hot carrier relaxation phenomena at the nanoscale, involving short-lived out-of-equilibrium electronic states over a very broad range of energies, in interaction with thermal electronic and phononic baths. These issues call for a comprehensive understanding of ultrafast hot electron dynamics in plasmonic nanostructures. This paper aims to review our contribution to the field: starting from the fundamental physics of plasmonic nanostructures, we first describe the experimental techniques used to probe hot electrons; we then introduce a numerical model of ultrafast nanoscale relaxation processes, and present examples in which experiments and modelling are combined, with the aim of designing novel optical functionalities enabled by ultrafast hot-electron dynamics.
- Published
- 2023
- Full Text
- View/download PDF
30. Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene
- Author
-
Maiuri Paolo, Knezevich Anna, Biancotto Chiara, De Marco Alex, Vardabasso Chiara, and Marcello Alessandro
- Subjects
Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Background The human immunodeficiency virus type 1 (HIV-1) favors integration in active genes of host chromatin. It is believed that transcriptional interference of the viral promoter over the endogenous gene or vice versa might occur with implications in HIV-1 post-integrative transcriptional latency. Results In this work a cell line has been transduced with a HIV-based vector and selected for Tat-inducible expression. These cells were found to carry a single silent integration in sense orientation within the second intron of the HMBOX1 gene. The HIV-1 Tat transactivator induced the viral LTR and repressed HMBOX1 expression independently of vector integration. Instead, single-cell quantitative in situ hybridization revealed that allele-specific transcription of HMBOX1 carrying the integrated provirus was not affected by the transactivation of the viral LTR in cis. Conclusion A major observation of the work is that the HIV-1 genome has inserted in genes that are also repressed by Tat and this could be an advantage for the virus during transcriptional reactivation. In addition, it has also been observed that transcription of the provirus and of the endogenous gene in which it is integrated may coexist at the same time in the same genomic location.
- Published
- 2008
- Full Text
- View/download PDF
31. A real-time view of the TAR:Tat:P-TEFb complex at HIV-1 transcription sites
- Author
-
Knezevich Anna, Bertrand Edouard, Boireau Stéphanie, Maiuri Paolo, Molle Dorothée, Marcello Alessandro, and Basyuk Eugenia
- Subjects
Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract HIV-1 transcription is tightly regulated: silent in long-term latency and highly active in acutely-infected cells. Transcription is activated by the viral protein Tat, which recruits the elongation factor P-TEFb by binding the TAR sequence present in nascent HIV-1 RNAs. In this study, we analyzed the dynamic of the TAR:Tat:P-TEFb complex in living cells, by performing FRAP experiments at HIV-1 transcription sites. Our results indicate that a large fraction of Tat present at these sites is recruited by Cyclin T1. We found that in the presence of Tat, Cdk9 remained bound to nascent HIV-1 RNAs for 71s. In contrast, when transcription was activated by PMA/ionomycin, in the absence of Tat, Cdk9 turned-over rapidly and resided on the HIV-1 promoter for only 11s. Thus, the mechanism of trans-activation determines the residency time of P-TEFb at the HIV-1 gene, possibly explaining why Tat is such a potent transcriptional activator. In addition, we observed that Tat occupied HIV-1 transcription sites for 55s, suggesting that the TAR:Tat:P-TEFb complex dissociates from the polymerase following transcription initiation, and undergoes subsequent cycles of association/dissociation.
- Published
- 2007
- Full Text
- View/download PDF
32. A planar polarized MYO6-DOCK7-RAC1 axis promotes tissue fluidification in mammary epithelia
- Author
-
Luca Menin, Janine Weber, Stefano Villa, Emanuele Martini, Elena Maspero, Carlos A. Niño, Valeria Cancila, Alessandro Poli, Paolo Maiuri, Andrea Palamidessi, Emanuela Frittoli, Fabrizio Bianchi, Claudio Tripodo, Kylie J. Walters, Fabio Giavazzi, Giorgio Scita, and Simona Polo
- Subjects
CP: Cell biology ,Biology (General) ,QH301-705.5 - Abstract
Summary: Tissue fluidification and collective motility are pivotal in regulating embryonic morphogenesis, wound healing, and tumor metastasis. These processes frequently require that each cell constituent of a tissue coordinates its migration activity and directed motion through the oriented extension of lamellipodium cell protrusions, promoted by RAC1 activity. While the upstream RAC1 regulators in individual migratory cells or leader cells during invasion or wound healing are well characterized, how RAC1 is controlled in follower cells remains unknown. Here, we identify a MYO6-DOCK7 axis essential for spatially restricting RAC1 activity in a planar polarized fashion in model tissue monolayers. The MYO6-DOCK7 axis specifically controls the extension of cryptic lamellipodia required to drive tissue fluidification and cooperative-mode motion in otherwise solid and static carcinoma cell collectives.
- Published
- 2023
- Full Text
- View/download PDF
33. Ring currents modulate optoelectronic properties of aromatic chromophores at 25 T
- Author
-
Kudisch, Bryan, Maiuri, Margherita, Moretti, Luca, Oviedo, Maria B, Wang, Leon, Oblinsky, Daniel G, Prud’homme, Robert K, Wong, Bryan M, McGill, Stephen A, and Scholes, Gregory D
- Subjects
magnetic fields ,ultrafast spectroscopy ,aromatic ring currents - Abstract
The properties of organic molecules can be influenced by magnetic fields, and these magnetic field effects are diverse. They range from inducing nuclear Zeeman splitting for structural determination in NMR spectroscopy to polaron Zeeman splitting organic spintronics and organic magnetoresistance. A pervasive magnetic field effect on an aromatic molecule is the aromatic ring current, which can be thought of as an induction of a circular current of π-electrons upon the application of a magnetic field perpendicular to the π-system of the molecule. While in NMR spectroscopy the effects of ring currents on the chemical shifts of nearby protons are relatively well understood, and even predictable, the consequences of these modified electronic states on the spectroscopy of molecules has remained unknown. In this work, we find that photophysical properties of model phthalocyanine compounds and their aggregates display clear magnetic field dependences up to 25 T, with the aggregates showing more drastic magnetic field sensitivities depending on the intermolecular interactions with the amplification of ring currents in stacked aggregates. These observations are consistent with ring currents measured in NMR spectroscopy and simulated in time-dependent density functional theory calculations of magnetic field-dependent phthalocyanine monomer and dimer absorption spectra. We propose that ring currents in organic semiconductors, which commonly comprise aromatic moieties, may present new opportunities for the understanding and exploitation of combined optical, electronic, and magnetic properties.
- Published
- 2020
34. Luigi Mittiga (a cura di), I love Platì. Cento piccoli film intorno un paese dell’Aspromonte - Voci da Platì. Nove narratori e ventitré racconti dal 1878 a oggi
- Author
-
Arduino Maiuri
- Subjects
Anthropology ,GN1-890 ,Ethnology. Social and cultural anthropology ,GN301-674 ,Sociology (General) ,HM401-1281 - Published
- 2023
- Full Text
- View/download PDF
35. New hormone receptor-positive breast cancer mouse cell line mimicking the immune microenvironment of anti-PD-1 resistant mammary carcinoma
- Author
-
Carlos Lopez-Otin, Christophe Klein, Chantal Desdouets, Guido Kroemer, Pierre Laurent-Puig, Jonathan Pol, Sophie Mouillet-Richard, Gautier Stoll, Isabelle Martins, Maria Perez-Lanzon, Vincent Carbonnier, Pierre Cordier, Fatima Domenica Elisa De Palma, Adriana Petrazzuolo, Floriane Arbaretaz, Khady Mangane, Helene Fohrer Ting, Juliette Paillet, Delphine Le Corre, Wenjjin Xiao, Marine Sroussi, and Maria Chiara Maiuri
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background Progress in breast cancer (BC) research relies on the availability of suitable cell lines that can be implanted in immunocompetent laboratory mice. The best studied mouse strain, C57BL/6, is also the only one for which multiple genetic variants are available to facilitate the exploration of the cancer-immunity dialog. Driven by the fact that no hormone receptor-positive (HR+) C57BL/6-derived mammary carcinoma cell lines are available, we decided to establish such cell lines.Methods BC was induced in female C57BL/6 mice using a synthetic progesterone analog (medroxyprogesterone acetate, MPA) combined with a DNA damaging agent (7,12-dimethylbenz[a]anthracene, DMBA). Cell lines were established from these tumors and selected for dual (estrogen+progesterone) receptor positivity, as well as transplantability into C57BL/6 immunocompetent females.Results One cell line, which we called B6BC, fulfilled these criteria and allowed for the establishment of invasive estrogen receptor-positive (ER+) tumors with features of epithelial to mesenchymal transition that were abundantly infiltrated by myeloid immune populations but scarcely by T lymphocytes, as determined by single-nucleus RNA sequencing and high-dimensional leukocyte profiling. Such tumors failed to respond to programmed cell death-1 (PD-1) blockade, but reduced their growth on treatment with ER antagonists, as well as with anthracycline-based chemotherapy, which was not influenced by T-cell depletion. Moreover, B6BC-derived tumors reduced their growth on CD11b blockade, indicating tumor sustainment by myeloid cells. The immune environment and treatment responses recapitulated by B6BC-derived tumors diverged from those of ER+ TS/A cell-derived tumors in BALB/C mice, and of ER– E0771 cell-derived and MPA/DMBA-induced tumors in C57BL/6 mice.Conclusions B6BC is the first transplantable HR+ BC cell line derived from C57BL/6 mice and B6BC-derived tumors recapitulate the complex tumor microenvironment of locally advanced HR+ BC naturally resistant to PD-1 immunotherapy.
- Published
- 2023
- Full Text
- View/download PDF
36. Regorafenib beyond the Second Line in Relapsed Glioblastoma: A Case Report and Literature Review
- Author
-
Mario Pirozzi, Marianna Caterino, Sergio Facchini, Alessia Zotta, Gaetana Messina, Raffaele Rauso, Antonello Sica, Donato Sciano, Gaetano Facchini, Michele Orditura, Teresa Somma, Francesco Maiuri, Paolo Cappabianca, Fortunato Ciardiello, and Morena Fasano
- Subjects
glioblastoma ,regorafenib ,advanced treatment lines ,long survival ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Glioblastoma multiforme (GBM) is one of the most frequent and aggressive primary tumors in the central nervous system, representing more than 60% of all brain tumors in adults. Primary GBM remains incurable with a poor prognosis both for limited therapeutic alternatives and for a high risk of progression or recurrence. In fact, at recurrence, the few treatment options available, and often characterized by limited effectiveness, have always been an Achilles’ heel. The recent approval of second line of regorafenib, a multikinase inhibitor, has given hope after several years of darkness for new therapies in the treatment of GBM. Indeed, in the REGOMA trial, a phase 2 study, regorafenib was the first drug to show a statistically significant improvement in median overall survival compared with lomustine group, usually used in the second-line treatment after temozolomide failure. We report a case of a 43-year-old patient affected by GBM in treatment with regorafenib in third line of therapy with good disease control and long PFS.
- Published
- 2022
- Full Text
- View/download PDF
37. An obesogenic feedforward loop involving PPARγ, acyl-CoA binding protein and GABAA receptor
- Author
-
Gerasimos Anagnostopoulos, Omar Motiño, Sijing Li, Vincent Carbonnier, Hui Chen, Valentina Sica, Sylvère Durand, Mélanie Bourgin, Fanny Aprahamian, Nitharsshini Nirmalathasan, Romain Donne, Chantal Desdouets, Marcelo Simon Sola, Konstantina Kotta, Léa Montégut, Flavia Lambertucci, Didier Surdez, Grossetête Sandrine, Olivier Delattre, Maria Chiara Maiuri, José Manuel Bravo-SanPedro, Isabelle Martins, and Guido Kroemer
- Subjects
Cytology ,QH573-671 - Abstract
Abstract Acyl-coenzyme-A-binding protein (ACBP), also known as a diazepam-binding inhibitor (DBI), is a potent stimulator of appetite and lipogenesis. Bioinformatic analyses combined with systematic screens revealed that peroxisome proliferator-activated receptor gamma (PPARγ) is the transcription factor that best explains the ACBP/DBI upregulation in metabolically active organs including the liver and adipose tissue. The PPARγ agonist rosiglitazone-induced ACBP/DBI upregulation, as well as weight gain, that could be prevented by knockout of Acbp/Dbi in mice. Moreover, liver-specific knockdown of Pparg prevented the high-fat diet (HFD)-induced upregulation of circulating ACBP/DBI levels and reduced body weight gain. Conversely, knockout of Acbp/Dbi prevented the HFD-induced upregulation of PPARγ. Notably, a single amino acid substitution (F77I) in the γ2 subunit of gamma-aminobutyric acid A receptor (GABAAR), which abolishes ACBP/DBI binding to this receptor, prevented the HFD-induced weight gain, as well as the HFD-induced upregulation of ACBP/DBI, GABAAR γ2, and PPARγ. Based on these results, we postulate the existence of an obesogenic feedforward loop relying on ACBP/DBI, GABAAR, and PPARγ. Interruption of this vicious cycle, at any level, indistinguishably mitigates HFD-induced weight gain, hepatosteatosis, and hyperglycemia.
- Published
- 2022
- Full Text
- View/download PDF
38. Gliosarcoma with direct involvement of the oculomotor nerve: Case report and literature review
- Author
-
Sergio Corvino, MD, Carmela Peca, MD, Giuseppe Corazzelli, MD, and Francesco Maiuri, MD
- Subjects
Gliosarcoma ,Oculomotor nerve ,Third cranial nerve ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
Gliosarcoma is a rare malignant brain tumor, characterized by a biphasic tissue pattern with alternating areas displaying glial and mesenchymal differentiation. We first report a case of temporo-mesial gliosarcoma, extended to the crural and ambient cisterns, with direct involvement of the ipsilateral third cranial nerve and encasement of anterior choroidal, posterior communicant and posterior cerebral arteries, presenting without symptoms of peripheral neuropathy. A 61-year-old woman with 1-month history of intense bilateral frontal-temporal headache resistant to pharmacological therapy and paresis of the left lower midface underwent surgical resection, through pterional trans-sylvian approach, of a right temporo-mesial gliosarcoma which directly involved the ipsilateral oculomotor nerve. Reported cases of gliomas with direct involvement of a cranial nerve, from the third to the twelfth, are very rare, whit no cases of gliosarcoma described. Because of its rarity, sometimes this entity is not considered as diagnostic hypothesis and is misdiagnosed, both during preoperative diagnostic evaluation and during the surgery. Gliosarcoma is a strong challenge for neurosurgeons and neurooncologists because of low incidence, poor prognosis and limited reported cases on literature. This case shows unique features for localization, pattern of growth and clinical presentation.
- Published
- 2022
- Full Text
- View/download PDF
39. Malignant intraventricular meningioma: literature review and case report
- Author
-
Maiuri, Francesco, Mariniello, Giuseppe, Barbato, Marcello, Corvino, Sergio, Guadagno, Elia, Chiariotti, Lorenzo, and Del Basso De Caro, Marialaura
- Published
- 2022
- Full Text
- View/download PDF
40. The transcription factor PREP1(PKNOX1) regulates nuclear stiffness, the expression of LINC complex proteins and mechanotransduction
- Author
-
Purushothaman, Divya, Bianchi, Laura F., Penkov, Dmitry, Poli, Alessandro, Li, Qingsen, Vermezovic, Jelena, Pramotton, Francesca M., Choudhary, Ramveer, Pennacchio, Fabrizio A., Sommariva, Elena, Foiani, Marco, Gauthier, Nils, Maiuri, Paolo, and Blasi, Francesco
- Published
- 2022
- Full Text
- View/download PDF
41. High-resolution crystal structure of a 20 kDa superfluorinated gold nanocluster
- Author
-
Pigliacelli, Claudia, Acocella, Angela, Díez, Isabel, Moretti, Luca, Dichiarante, Valentina, Demitri, Nicola, Jiang, Hua, Maiuri, Margherita, Ras, Robin H. A., Bombelli, Francesca Baldelli, Cerullo, Giulio, Zerbetto, Francesco, Metrangolo, Pierangelo, and Terraneo, Giancarlo
- Published
- 2022
- Full Text
- View/download PDF
42. Ultrafast All-Optical Metasurfaces: Challenges and New Frontiers.
- Author
-
Maiuri, Margherita, Schirato, Andrea, Cerullo, Giulio, and Della Valle, Giuseppe
- Published
- 2024
- Full Text
- View/download PDF
43. Watching the coherent birth of polaron pairs in conjugated polymers
- Author
-
De Sio, Antonietta, Troiani, Filippo, Maiuri, Margherita, Réhault, Julien, Sommer, Ephraim, Lim, James, Huelga, Susana F., Plenio, Martin B., Rozzi, Carlo Andrea, Cerullo, Giulio, Molinari, Elisa, and Lienau, Christoph
- Subjects
Condensed Matter - Mesoscale and Nanoscale Physics ,Condensed Matter - Soft Condensed Matter ,Physics - Chemical Physics ,Quantum Physics - Abstract
Organic semiconductors have the remarkable property that their optical excitation not only generates charge-neutral electron-hole pairs (excitons) but also charge-separated polaron pairs with high yield. The microscopic mechanisms underlying this charge separation have been debated for many years. Here we use ultrafast two-dimensional electronic spectroscopy to study the dynamics of polaron pair formation in a prototypical polymer thin film on a sub-20-fs time scale. We observe multi-period peak oscillations persisting for up to about 1 ps as distinct signatures of vibronic quantum coherence at room temperature. The measured two-dimensional spectra show pronounced peak splittings revealing that the elementary optical excitations of this polymer are hybridized exciton-polaron-pairs, strongly coupled to a dominant underdamped vibrational mode. Coherent vibronic coupling induces ultrafast polaron pair formation, accelerates the charge separation dynamics and makes it insensitive to disorder. These findings open up new perspectives for tailoring light-to-current conversion in organic materials.
- Published
- 2016
- Full Text
- View/download PDF
44. Update on the Diagnosis and Management of Meningiomas
- Author
-
Francesco Maiuri and Marialaura Del Basso de Caro
- Subjects
n/a ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
This series of five articles (one original article and four reviews) focuses on the most recent and interesting research studies on the biomolecular and radiological diagnosis and the surgical and medical management of meningiomas [...]
- Published
- 2023
- Full Text
- View/download PDF
45. Trial Watch: combination of tyrosine kinase inhibitors (TKIs) and immunotherapy
- Author
-
Adriana Petrazzuolo, M. Chiara Maiuri, Laurence Zitvogel, Guido Kroemer, and Oliver Kepp
- Subjects
Immunotherapy ,lung cancer ,checkpoint blockade ,targeted therapy ,personalized medicine ,Immunologic diseases. Allergy ,RC581-607 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
The past decades witnessed the clinical employment of targeted therapies including but not limited to tyrosine kinase inhibitors (TKIs) that restrain a broad variety of pro-tumorigenic signals. TKIs can be categorized into (i) agents that directly target cancer cells, (ii) normalize angiogenesis or (iii) affect cells of the hematologic lineage. However, a clear distinction of TKIs based on this definition is limited by the fact that many TKIs designed to inhibit cancer cells have also effects on immune cells that are being discovered. Additionally, TKIs originally designed to target hematological cancers exhibit bioactivities on healthy cells of the same hematological lineage. TKIs have been described to improve immune recognition and cancer immunosurveillance, providing the scientific basis to combine TKIs with immunotherapy. Indeed, combination of TKIs with immunotherapy showed synergistic effects in preclinical models and clinical trials and some combinations of TKIs normalizing angiogenesis with immune checkpoint blocking antibodies have already been approved by the FDA for cancer therapy. However, the identification of appropriate drug combinations as well as optimal dosing and scheduling needs to be improved in order to obtain tangible progress in cancer care. This Trial Watch summarizes active clinical trials combining TKIs with various immunotherapeutic strategies to treat cancer patients.
- Published
- 2022
- Full Text
- View/download PDF
46. Pharmacological inhibitors of anaplastic lymphoma kinase (ALK) induce immunogenic cell death through on-target effects
- Author
-
Adriana Petrazzuolo, Maria Perez-Lanzon, Isabelle Martins, Peng Liu, Oliver Kepp, Véronique Minard-Colin, Maria Chiara Maiuri, and Guido Kroemer
- Subjects
Cytology ,QH573-671 - Abstract
Abstract Immunogenic cell death (ICD) is clinically relevant because cytotoxicants that kill malignant cells via ICD elicit anticancer immune responses that prolong the effects of chemotherapies beyond treatment discontinuation. ICD is characterized by a series of stereotyped changes that increase the immunogenicity of dying cells: exposure of calreticulin on the cell surface, release of ATP and high mobility group box 1 protein, as well as a type I interferon response. Here, we examined the possibility that inhibition of an oncogenic kinase, anaplastic lymphoma kinase (ALK), might trigger ICD in anaplastic large cell lymphoma (ALCL) in which ALK is activated due to a chromosomal translocation. Multiple lines of evidence plead in favor of specific ICD-inducing effects of crizotinib and ceritinib in ALK-dependent ALCL: (i) they induce ICD stigmata at pharmacologically relevant, low concentrations; (ii) can be mimicked in their ICD-inducing effects by ALK knockdown; (iii) lose their effects in the context of resistance-conferring ALK mutants; (iv) ICD-inducing effects are mimicked by inhibition of the signal transduction pathways operating downstream of ALK. When ceritinib-treated murine ALK-expressing ALCL cells were inoculated into the left flank of immunocompetent syngeneic mice, they induced an immune response that slowed down the growth of live ALCL cells implanted in the right flank. Although ceritinib induced a transient shrinkage of tumors in lymphoma-bearing mice, irrespective of their immunocompetence, relapses occurred more frequently in the context of immunodeficiency, reducing the effects of ceritinib on survival by approximately 50%. Complete cure only occurred in immunocompetent mice and conferred protection to rechallenge with the same ALK-expressing lymphoma but not with another unrelated lymphoma. Moreover, immunotherapy with PD-1 blockade tended to increase cure rates. Altogether, these results support the contention that specific ALK inhibition stimulates the immune system by inducing ICD in ALK-positive ALCL.
- Published
- 2021
- Full Text
- View/download PDF
47. Author Correction: Tissue fluidification promotes a cGAS–STING cytosolic DNA response in invasive breast cancer
- Author
-
Frittoli, Emanuela, Palamidessi, Andrea, Iannelli, Fabio, Zanardi, Federica, Villa, Stefano, Barzaghi, Leonardo, Abdo, Hind, Cancila, Valeria, Beznoussenko, Galina V., Della Chiara, Giulia, Pagani, Massimiliano, Malinverno, Chiara, Bhattacharya, Dipanjan, Pisati, Federica, Yu, Weimiao, Galimberti, Viviana, Bonizzi, Giuseppina, Martini, Emanuele, Mironov, Alexander A., Gioia, Ubaldo, Ascione, Flora, Li, Qingsen, Havas, Kristina, Magni, Serena, Lavagnino, Zeno, Pennacchio, Fabrizio Andrea, Maiuri, Paolo, Caponi, Silvia, Mattarelli, Maurizio, Martino, Sabata, d’Adda di Fagagna, Fabrizio, Rossi, Chiara, Lucioni, Marco, Tancredi, Richard, Pedrazzoli, Paolo, Vecchione, Andrea, Petrini, Cristiano, Ferrari, Francesco, Lanzuolo, Chiara, Bertalot, Giovanni, Nader, Guilherme, Foiani, Marco, Piel, Matthieu, Cerbino, Roberto, Giavazzi, Fabio, Tripodo, Claudio, and Scita, Giorgio
- Published
- 2023
- Full Text
- View/download PDF
48. Effects of acyl-coenzyme A binding protein (ACBP)/diazepam-binding inhibitor (DBI) on body mass index
- Author
-
Adrien Joseph, Hui Chen, Gerasimos Anagnostopoulos, Léa Montégut, Antoine Lafarge, Omar Motiño, Maria Castedo, Maria Chiara Maiuri, Karine Clément, Safae Terrisse, Anne Laure Martin, Ines Vaz-Luis, Fabrice Andre, Franziska Grundler, Françoise Wilhelmi de Toledo, Frank Madeo, Laurence Zitvogel, François Goldwasser, Benoit Blanchet, Frédéric Fumeron, Ronan Roussel, Isabelle Martins, and Guido Kroemer
- Subjects
Cytology ,QH573-671 - Abstract
Abstract In mice, the plasma concentrations of the appetite-stimulatory and autophagy-inhibitory factor acyl-coenzyme A binding protein (ACBP, also called diazepam-binding inhibitor, DBI) acutely increase in response to starvation, but also do so upon chronic overnutrition leading to obesity. Here, we show that knockout of Acbp/Dbi in adipose tissue is sufficient to prevent high-fat diet-induced weight gain in mice. We investigated ACBP/DBI plasma concentrations in several patient cohorts to discover a similar dual pattern of regulation. In relatively healthy subjects, ACBP/DBI concentrations independently correlated with body mass index (BMI) and age. The association between ACBP/DBI and BMI was lost in subjects that underwent major weight gain in the subsequent 3–9 years, as well as in advanced cancer patients. Voluntary fasting, undernutrition in the context of advanced cancer, as well as chemotherapy were associated with an increase in circulating ACBP/DBI levels. Altogether, these results support the conclusion that ACBP/DBI may play an important role in body mass homeostasis as well as in its failure.
- Published
- 2021
- Full Text
- View/download PDF
49. Correction: An obesogenic feedforward loop involving PPARγ, acyl-CoA binding protein and GABA receptor
- Author
-
Anagnostopoulos, Gerasimos, Motiño, Omar, Li, Sijing, Carbonnier, Vincent, Chen, Hui, Sica, Valentina, Durand, Sylvère, Bourgin, Mélanie, Aprahamian, Fanny, Nirmalathasan, Nitharsshini, Donne, Romain, Desdouets, Chantal, Sola, Marcelo Simon, Kotta, Konstantina, Montégut, Léa, Lambertucci, Flavia, Surdez, Didier, Sandrine, Grossetête, Delattre, Olivier, Maiuri, Maria Chiara, Bravo-San Pedro, José Manuel, Martins, Isabelle, and Kroemer, Guido
- Published
- 2022
- Full Text
- View/download PDF
50. An obesogenic feedforward loop involving PPARγ, acyl-CoA binding protein and GABAA receptor
- Author
-
Anagnostopoulos, Gerasimos, Motiño, Omar, Li, Sijing, Carbonnier, Vincent, Chen, Hui, Sica, Valentina, Durand, Sylvère, Bourgin, Mélanie, Aprahamian, Fanny, Nirmalathasan, Nitharsshini, Donne, Romain, Desdouets, Chantal, Sola, Marcelo Simon, Kotta, Konstantina, Montégut, Léa, Lambertucci, Flavia, Surdez, Didier, Sandrine, Grossetête, Delattre, Olivier, Maiuri, Maria Chiara, Bravo-San Pedro, José Manuel, Martins, Isabelle, and Kroemer, Guido
- Published
- 2022
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.