Exopolysaccharides (EPSs) may be regarded as extracellular metabolites of living organisms (bacteria, fungi, algae, plants and animals, etc.) associated with adaptation, survival and functionalities. Exopolysaccharides (EPSs) produced from fungi have been documented as high worth molecules for the recent few decades. A wide array of fungal exopolysaccharides is reported to exhibit numerous applications in pharmaceuticals, industries, medicine, foods and other sectors, viz. scleroglucan, botryosphaeran, pestan, pullulan, etc. Although fungal EPSs are exceptionally relevant, to date data concerning fungal biosynthesis is scant and a broad chase for new fungal species which could create novel EPSs is as yet required. As a rule, the molecular weight disparities and sugar syntheses of fungal EPSs are needy to culture medium synthesis and distinctive states of being given during maturation. An accentuation is likewise given to drilling down various parasitic strains that can deliver effective EPSs. The variable synthetic and biochemical engineering that describes an EPS preset its organic usefulness and potential biotechnological benefits. It is agreeable to hereditary, biotechnological and biochemical mobility for sought bioactivity or application during their production and extraction. A brief idea regarding fungal EPS is an insight into this chapter. The overall chapter describes the concept of fungal EPS, their production confirmation and wide applications.