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6. Tailoring polishing steps for effective removal of polysorbate‐degrading host cell proteins in antibody purification.

Author

Maier, Melanie, Schneider, Stefan, Weiss, Linus, Fischer, Simon, Lakatos, Daniel, Studts, Joey, and Franzreb, Matthias

Abstract

Ensuring the quality and safety of biopharmaceutical products requires the effective separation of monoclonal antibodies (mAbs) from host cell proteins (HCPs). A major challenge in this field is the enzymatic hydrolysis of polysorbates (PS) in drug products. This study addresses this issue by investigating the removal of polysorbate‐degrading HCPs during the polishing steps of downstream purification, an area where knowledge about individual HCP behavior is still limited. We investigated the separation of different mAb formats from four individual polysorbate degrading hydrolases (CES1F, CES2C, LPLA2, and PAF‐AH) using cation exchange (CEX) and mixed‐mode chromatography (MMC) polishing steps. Our research identified a key challenge: The similar elution behavior of mAbs and HCPs during chromatographic separation. To investigate this phenomenon, we performed high‐throughput binding screenings for recombinant polysorbate degrading hydrolases and representative mAb candidates on CEX and MMC chromatography resins. We then employed a three‐step strategy that also served as a scale‐up process, optimizing separation conditions and leading to the successful removal of specific HCPs while maintaining high mAb recovery rates (>96%). This strategy involved the use of surface response models and miniature columns for screening, followed by validation on larger columns using a chromatography system. Our results highlight the critical role of the inherent properties of mAbs for successful separation from HCPs. These results underscore the need to tailor the purification process to leverage the slight differences in binding behavior and elution profiles between mAbs and specific HCPs. This approach lays the foundation for developing more effective strategies for overcoming the challenge of enzymatic polysorbate degradation, paving the way for improved quality and safety in biopharmaceutical products. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Respiratory Syncytial Virus in Adult Patients at a Tertiary Care Hospital in Germany: Clinical Features and Molecular Epidemiology of the Fusion Protein in the Severe Respiratory Season of 2022/2023

Author

Hönemann, Mario, primary, Maier, Melanie, additional, Frille, Armin, additional, Thiem, Stephanie, additional, Bergs, Sandra, additional, Williams, Thomas C., additional, Mas, Vicente, additional, Lübbert, Christoph, additional, and Pietsch, Corinna, additional

Published
2024
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13. Epidemiological and clinical insights into the enterovirus D68 upsurge in Europe 2021/22 and the emergence of novel B3-derived lineages, ENPEN multicentre study

Author

Simoes, Margarida Pires, primary, Hodcroft, Emma B, additional, Simmonds, Peter, additional, Albert, Jan, additional, Alidjinou, Enagon K, additional, Ambert-Balay, Katia, additional, Andrés, Cristina, additional, Antón, Andrés, additional, Auvray, Christelle, additional, Bailly, Jean-Luc, additional, Baldanti, Fausto, additional, Bastings, Capser, additional, Beard, Stuart, additional, Pereira, Carla Berengua, additional, Berginc, Natasa, additional, Bloemen, Mandy, additional, Blomqvist, Soile, additional, Bosma, Froukje, additional, Böttcher, Sindy, additional, Bubba, Laura, additional, Buderus, Stafan, additional, Cabrerizo, Maria, additional, Calvo, Christina, additional, Celma, Christina, additional, Ceriotti, Ferruccio, additional, Clark, Gemma, additional, Costa, Inës, additional, Coste-Burel, Marianne, additional, Couderé, Karen, additional, Cremer, Jeroen, additional, del Cuerpo Casas, Margarita, additional, Daehne, Theo, additional, de Beer, Jessica, additional, de Ceano-Vivas, Maria, additional, De Gascun, Cillian, additional, de Rougemont, Alexis, additional, Dean, Johnathan, additional, Dembinski, Jennifer L, additional, Diedrich, Sabine, additional, Diez-Domingo, Javier, additional, Dillner, Lena, additional, Dorenberg, Dagny H, additional, Ducancelle, Alexandra, additional, Dudman, Susanne, additional, Dyrdak, Robert, additional, Eis-Huebinger, Anna-Maria, additional, Falces-Romero, Iker, additional, Farkas, Agnes, additional, Feeney, Susan, additional, Fernandez-Garcia, Maria D, additional, Flipse, Jacky, additional, Franck, Kristina T, additional, Galli, Cristina, additional, Garrigue, Isabelle, additional, Geeraedts, Felix, additional, Georgieva, Irina, additional, Giardina, Federica, additional, Guiomar, Raquel, additional, Hauzenberger, Elenor, additional, Heikens, Esther, additional, Henquell, Cécille, additional, Hober, Didier, additional, Hönemann, Amrio, additional, Howson-Wells, Hannah, additional, Hruškar, Željka, additional, Ikonen, Niina, additional, Imbert, Berthemarie, additional, Jansz, Arjan R, additional, Jeannoël, Marion, additional, Jiřincová, Helena, additional, Josset, Laurence, additional, Keeren, Kathrin, additional, Kramer-Lindhout, Naomie, additional, Krokstad, Sidsel, additional, Lazrek, Mouna, additional, Le Guillou-Guillemette, Hélène, additional, Lefeuvre, Caroline, additional, Lind, Andreas, additional, Lunar, Maja M, additional, Maier, Melanie, additional, Marque-Juillet, Stéphanie, additional, McClure, C Patrick, additional, McKenna, James, additional, Meijer, Adam, additional, Menasalvas Ruiz, Ana, additional, Mengual-Chuliá, Beatriz, additional, Midgley, Sofie, additional, Mirand, Audrey, additional, Molenkamp, Richard, additional, Montes, Milagrosa, additional, Moreno-Docón, Antonio, additional, Morley, Ursula, additional, Murk, Jean-Luc, additional, Navascués-Ortega, Ana, additional, Nijhuis, Roel, additional, Nikolaeva-Glomb, Lubomira, additional, Nordbø, Svein A, additional, Numanovic, Sanela, additional, Oggioni, Massimo, additional, Oñate Vergara, Eider, additional, Pacaud, Jordi, additional, Pacreau, Marie L, additional, Panning, Marcus, additional, Pariani, Elena, additional, Pekova, Lili, additional, Pellegrinelli, Laura, additional, Petrovec, Miroslav, additional, Pietsch, Corinna, additional, Pilorge, Léa, additional, Piñeiro, Luis, additional, Piralla, Antonio, additional, Poljak, Mario, additional, Prochazka, Birgit, additional, Rabella, Nuria, additional, Rahamat-Langendoen, Janette C, additional, Rainetova, petra, additional, Reynders, Marijke, additional, Riezebos-Brilman, Annelies, additional, Roorda, Lieuwe, additional, Savolainen-Kopra, Carita, additional, Schuffenecker, Isabelle, additional, Smeets, Leo C, additional, Stoyanova, Ayse, additional, Stefic, Karl, additional, Swanink, Caroline, additional, Tabain, Irena, additional, Tjhie, Jeroen, additional, Thouault, Luc, additional, Tumiotto, Camille, additional, Uceda Renteria, Sara, additional, Uršič, Tina, additional, Vallet, Sophie, additional, Van Ranst, Marc, additional, Van Wunnik, Peter, additional, Verweij, Jaco J, additional, Vila, Jorgina, additional, Wintermans, Bas, additional, Wollants, Elke, additional, Wolthers, Katja C, additional, Xavier López-Labrado, F, additional, Fischer, Thea Kolsen, additional, Harvala, Heli, additional, and Benschop, Kimberley S M, additional

Published
2024
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14. Smart hospital: achieving interoperability and raw data collection from medical devices in clinical routine

Author

Martens, Eimo, primary, Haase, Hans-Ulrich, additional, Mastella, Giulio, additional, Henkel, Andreas, additional, Spinner, Christoph, additional, Hahn, Franziska, additional, Zou, Congyu, additional, Fava Sanches, Augusto, additional, Allescher, Julia, additional, Heid, Daniel, additional, Strauss, Elena, additional, Maier, Melanie-Maria, additional, Lachmann, Mark, additional, Schmidt, Georg, additional, Westphal, Dominik, additional, Haufe, Tobias, additional, Federle, David, additional, Rueckert, Daniel, additional, Boeker, Martin, additional, Becker, Matthias, additional, Laugwitz, Karl-Ludwig, additional, Steger, Alexander, additional, and Müller, Alexander, additional

Published
2024
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15. COVID-19 severity correlates with airway epithelium–immune cell interactions identified by single-cell analysis

Author

Chua, Robert Lorenz, Lukassen, Soeren, Trump, Saskia, Hennig, Bianca P., Wendisch, Daniel, Pott, Fabian, Debnath, Olivia, Thürmann, Loreen, Kurth, Florian, Völker, Maria Theresa, Kazmierski, Julia, Timmermann, Bernd, Twardziok, Sven, Schneider, Stefan, Machleidt, Felix, Müller-Redetzky, Holger, Maier, Melanie, Krannich, Alexander, Schmidt, Sein, Balzer, Felix, Liebig, Johannes, Loske, Jennifer, Suttorp, Norbert, Eils, Jürgen, Ishaque, Naveed, Liebert, Uwe Gerd, von Kalle, Christof, Hocke, Andreas, Witzenrath, Martin, Goffinet, Christine, Drosten, Christian, Laudi, Sven, Lehmann, Irina, Conrad, Christian, Sander, Leif-Erik, and Eils, Roland

Published
2020
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16. Recommendations for enterovirus diagnostics and characterisation within and beyond Europe

Author

Harvala, Heli, Broberg, Eeva, Benschop, Kimberley, Berginc, Natasa, Ladhani, Shamez, Susi, Petri, Christiansen, Claus, McKenna, James, Allen, David, Makiello, Phoebe, McAllister, Georgina, Carmen, Mirabelli, Zakikhany, Katherina, Dyrdak, Robert, Nielsen, Xiaohui, Madsen, Tina, Paul, Joel, Moore, Catherine, von Eije, Karin, Piralla, Antonio, Carlier, Mieke, Vanoverschelde, Laura, Poelman, Randy, Anton, Andrés, López-Labrador, F. Xavier, Pellegrinelli, Laura, Keeren, Kathrin, Maier, Melanie, Cassidy, Hayley, Derdas, Stavros, Savolainen-Kopra, Carita, Diedrich, Sabine, Nordbø, Svein, Buesa, Javier, Bailly, Jean-Luc, Baldanti, Fausto, MacAdam, Andrew, Mirand, Audrey, Dudman, Susanne, Schuffenecker, Isabelle, Kadambari, Seilesh, Neyts, Johan, Griffiths, Michael J., Richter, Jan, Margaretto, Cristina, Govind, Sheila, Morley, Ursula, Adams, Ortwin, Krokstad, Sidsel, Dean, Jonathan, Pons-Salort, Margarita, Prochazka, Birgit, Cabrerizo, Maria, Majumdar, Manasi, Nebbia, Gaia, Wiewel, Maryse, Cottrell, Simon, Coyle, Peter, Martin, Javier, Moore, Catrin, Midgley, Sofie, Horby, Peter, Wolthers, Katja, Simmonds, Peter, Niesters, Hubert, and Fischer, Thea K.

Published
2018
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18. A Specific Pattern of Routine Cerebrospinal Fluid Parameters Might Help to Identify Cases of West Nile Virus Neuroinvasive Disease.

Author

Pelz, Johann Otto, Mühlberg, Christoph, Friedrich, Isabel, Weidhase, Lorenz, Zimmermann, Silke, Maier, Melanie, and Pietsch, Corinna

Subjects
WEST Nile fever, CEREBROSPINAL fluid, CEREBROSPINAL fluid examination, WEST Nile virus, IMMUNOGLOBULIN M, VIRUS diseases
Abstract

Background: Viral meningitis/encephalitis (ME) is a rare but potentially harmful disease. The prompt identification of the respective virus is important to guide not only treatment but also potential public health countermeasures. However, in about 40% of cases, no virus is identified despite an extensive diagnostic workup. The aim of the present study was to analyze demographic, seasonal, and routine cerebrospinal fluid (CSF) parameters in cases of viral ME and assess their utility for the prediction of the causative virus. Methods: Demographic data, season, and routine CSF parameters (total leucocytes, CSF cell differentiation, age-adjusted CSF/serum albumin ratio, and total immunoglobulin ratios) were retrospectively assessed in cases of viral ME. Results: In total, 156 cases of acute viral ME (74 female, median age 40.0 years) were treated at a tertiary-care hospital in Germany. Specific viral infections were detected in 93 (59.6%) cases. Of these, 14 (9.0%) cases were caused by herpes simplex virus (HSV), 36 (23.1%) by varicella-zoster virus (VZV), 27 (17.3%) by enteroviruses, 9 (5.8%) by West Nile virus (WNV), and 7 (4.5%) by other specific viruses. Additionally, 64 (41.0%) cases of ME of unknown viral etiology were diagnosed. Cases of WNV ME were older, predominantly male, showed a severe disruption of the blood–CSF–barrier, a high proportion of neutrophils in CSF, and an intrathecal total immunoglobulin M synthesis in the first CSF sample. In a multinominal logistic regression analysis, the accuracy of these CSF parameters together with age and seasonality was best for the prediction of WNV (87.5%), followed by unknown viral etiology (66.7%), VZV (61.8%), and enteroviruses (51.9%). Conclusions: Cases with WNV ME showed a specific pattern of routine CSF parameters and demographic data that allowed for their identification with good accuracy. These findings might help to guide the diagnostic workup in cases with viral ME, in particular allowing the timely identification of cases with ME due to WNV. [ABSTRACT FROM AUTHOR]

Published
2024
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19. In-Depth Analysis of the Re-Emergence of Respiratory Syncytial Virus at a Tertiary Care Hospital in Germany in the Summer of 2021 after the Alleviation of Non-Pharmaceutical Interventions Due to the SARS-CoV-2 Pandemic

Author

Hönemann, Mario, primary, Thiem, Stephanie, additional, Bergs, Sandra, additional, Berthold, Tom, additional, Propach, Christian, additional, Siekmeyer, Manuela, additional, Frille, Armin, additional, Wallborn, Tillmann, additional, Maier, Melanie, additional, and Pietsch, Corinna, additional

Published
2023
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28. Efficacy and Safety of Bulevirtide plus Tenofovir Disoproxil Fumarate in Real-World Patients with Chronic Hepatitis B and D Co-Infection

Author

Herta, Toni, Hahn, Magdalena, Maier, Melanie, Fischer, Janett, Niemeyer, Johannes, Hönemann, Mario, Böhlig, Albrecht, Gerhardt, Florian, Schindler, Aaron, Schumacher, Jonas, Berg, Thomas, Wiegand, Johannes, van Bömmel, Florian, Herta, Toni, Hahn, Magdalena, Maier, Melanie, Fischer, Janett, Niemeyer, Johannes, Hönemann, Mario, Böhlig, Albrecht, Gerhardt, Florian, Schindler, Aaron, Schumacher, Jonas, Berg, Thomas, Wiegand, Johannes, and van Bömmel, Florian

Abstract

Background: The hepatitis B and D virus (HBV/HDV) hepatocyte entry inhibitor bulevirtide (BLV) has been available in Europe since July 2020, after the registrational trial MYR202. Real-life data on the efficacy and safety of BLV are sparse. Methods: We have analysed the course of treatment with BLV (2 mg/day) plus tenofovir disoproxil fumarate (TDF) (245 mg/day) in patients with chronic hepatitis delta (CHD). Virologic (≥2 log reduction in HDV RNA or suppression of HDV RNA below the lower limit of detection) and biochemical (normalisation of serum ALT) treatment responses after 24 weeks were defined according to the MYR202 trial. Results: Seven patients were recruited (four with liver cirrhosis Child–Pugh A). After 24 weeks, a virologic response was observed in five of seven and a biochemical response was seen in three of six patients with elevated serum ALT at baseline. Extended treatment data > 48 weeks were available in three cases: two presented with continuous virologic and biochemical responses and in one individual an HDV-RNA breakthrough was observed. Adverse effects were not recorded. Conclusions: The first real-life data of the approved dosage of 2 mg of BLV in combination with TDF confirm the safety, tolerability, and efficacy of the registrational trial MYR202 for a treatment period of 24 weeks and beyond.

Published
2022

29. Flow-Cytometry Intracellular Detection and Quantification of HIV1 p24 Antigen and Immunocheckpoint Molecules in T Cells among HIV/AIDS Patients

Author

Tessema,Belay, Boldt,Andreas, König,Brigitte, Maier,Melanie, Sack,Ulrich, Tessema,Belay, Boldt,Andreas, König,Brigitte, Maier,Melanie, and Sack,Ulrich

Abstract

Belay Tessema,1– 3 Andreas Boldt,2 Brigitte König,3 Melanie Maier,4 Ulrich Sack2 1Department of Medical Microbiology, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia; 2Institute of Clinical Immunology, Faculty of Medicine, University of Leipzig, Leipzig, Germany; 3Institute of Medical Microbiology and Virology, Faculty of Medicine, University of Leipzig, Leipzig, Germany; 4Department of Virology, Institute of Medical Microbiology and Virology, Faculty of Medicine, University of Leipzig, Leipzig, GermanyCorrespondence: Belay Tessema, Department of Medical Microbiology, College of Medicine and Health Sciences, University of Gondar, 196, Gondar, Ethiopia, Tel +251-91-930-6918, Email bt1488@yahoo.comIntroduction: HIV p24 antigen-positive T cells measured by flow cytometry (FCM) correlate directly with HIV viral load, inversely with CD4+ T cells, and decrease with antiretroviral therapy (ART). However, the sensitivity of FCM assays depends on the protocol of intracellular staining. Therefore, this study aimed to evaluate the diagnostic performance of our FCM protocol for detection of HIV p24-positive T cells and measure the level of immunocheckpoint molecules (PD1 and TIM3) in T cells.Methods: The study was conducted at the University of Leipzig hospital between January 2020 and November 2020. Viremic and ART-suppressed HIV-positive patients and negative controls were included in this study. HIV1 p24 KC57-, p24 28B7-, PD1-, and TIM3-positive CD4 and CD3 T cells were analyzed from whole blood using a BD FACS Canto II flow cytometer equipped with FACSDiva software. HIV1 p24 antigen FCM results were compared with HIV1 RNA viral load results measured by Alinity M assays on the fully automated random-access platform. We analyzed the data using SPSS 20.Results: The absolute CD4+ and CD4+:CD8+ T-cells ratio showed a significant inverse correlation with HIV1 viral load. Moreover, the absolute CD4+ T-cells count showed a significant inverse corr

Published
2022

30. Efficacy and Safety of Bulevirtide plus Tenofovir Disoproxil Fumarate in Real-World Patients with Chronic Hepatitis B and D Co-Infection

Author

Herta, Toni, primary, Hahn, Magdalena, additional, Maier, Melanie, additional, Fischer, Janett, additional, Niemeyer, Johannes, additional, Hönemann, Mario, additional, Böhlig, Albrecht, additional, Gerhardt, Florian, additional, Schindler, Aaron, additional, Schumacher, Jonas, additional, Berg, Thomas, additional, Wiegand, Johannes, additional, and van Bömmel, Florian, additional

Published
2022
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33. Re-emergence of enterovirus D68 in Europe after easing the COVID-19 lockdown, September 2021

Author

Benschop, Kimberley Sm, Albert, Jan, Anton, Andres, Andrés, Cristina, Aranzamendi, Maitane, Armannsdóttir, Brynja, Bailly, Jean-Luc, Baldanti, Fausto, Baldvinsdóttir, Guðrún Erna, Beard, Stuart, Berginc, Natasa, Böttcher, Sindy, Blomqvist, Soile, Bubba, Laura, Calvo, Cristina, Cabrerizo, Maria, Cavallero, Annalisa, Celma, Cristina, Ceriotti, Ferruccio, Costa, Inês, Cottrell, Simon, Del Cuerpo, Margarita, Dean, Jonathan, Dembinski, Jennifer L., Diedrich, Sabine, Diez-Domingo, Javier, Dorenberg, Dagnyhaug, Duizer, Erwin, Dyrdak, Robert, Fanti, Diana, Farkas, Agnes, Feeney, Susan, Flipse, Jacky, Gascun, Cillian, Galli, Cristina, Georgieva, Irina, Gifford, Laura, Guiomar, Raquel, Hönemann, Mario, Ikonen, Niina, Jeannoël, Marion, Josset, Laurence, Keeren, Kathrin, López-Labrador, F. Xavier, Maier, Melanie, Mckenna, James, Meijer, Adam, Mengual-Chuliá, Beatriz, Midgley, Sofie E., Mirand, Audrey, Montes, Milagrosa, Moore, Catherine, Morley, Ursula, Murk, Jean-Luc, Nikolaeva-Glomb, Lubomira, Numanovic, Sanela, Oggioni, Massimo, Palminha, Paula, Pariani, Elena, Pellegrinelli, Laura, Piralla, Antonio, Pietsch, Corinna, Piñeiro, Luis, Rabella, Núria, Rainetova, Petra, Sara Colonia UCEDA RENTERIA, Romero, María P., Reynders, Marijke, Roorda, Lieuwe, Savolainen-Kopra, Carita, Schuffenecker, Isabelle, Soynova, Aysa, Swanink, Caroline Ma, Ursic, Tina, Verweij, Jaco J., Vila, Jorgina, Vuorinen, Tytti, Simmonds, Peter, Fischer, Thea K., and Harvala, Heli

Subjects
enterovirus, respiratory infection, surveillance, typing, acute flaccid myelitis, enterovirus D68
Published
2021

36. Re-emergence of enterovirus D68 in Europe after easing the COVID-19 lockdown, September 2021

Author

Benschop, Kimberley SM, primary, Albert, Jan, additional, Anton, Andres, additional, Andrés, Cristina, additional, Aranzamendi, Maitane, additional, Armannsdóttir, Brynja, additional, Bailly, Jean-Luc, additional, Baldanti, Fausto, additional, Baldvinsdóttir, Guðrún Erna, additional, Beard, Stuart, additional, Berginc, Natasa, additional, Böttcher, Sindy, additional, Blomqvist, Soile, additional, Bubba, Laura, additional, Calvo, Cristina, additional, Cabrerizo, Maria, additional, Cavallero, Annalisa, additional, Celma, Cristina, additional, Ceriotti, Ferruccio, additional, Costa, Inês, additional, Cottrell, Simon, additional, del Cuerpo, Margarita, additional, Dean, Jonathan, additional, Dembinski, Jennifer L, additional, Diedrich, Sabine, additional, Diez-Domingo, Javier, additional, Dorenberg, DagnyHaug, additional, Duizer, Erwin, additional, Dyrdak, Robert, additional, Fanti, Diana, additional, Farkas, Agnes, additional, Feeney, Susan, additional, Flipse, Jacky, additional, De Gascun, Cillian, additional, Galli, Cristina, additional, Georgieva, Irina, additional, Gifford, Laura, additional, Guiomar, Raquel, additional, Hönemann, Mario, additional, Ikonen, Niina, additional, Jeannoël, Marion, additional, Josset, Laurence, additional, Keeren, Kathrin, additional, López-Labrador, F Xavier, additional, Maier, Melanie, additional, McKenna, James, additional, Meijer, Adam, additional, Mengual-Chuliá, Beatriz, additional, Midgley, Sofie E, additional, Mirand, Audrey, additional, Montes, Milagrosa, additional, Moore, Catherine, additional, Morley, Ursula, additional, Murk, Jean-Luc, additional, Nikolaeva-Glomb, Lubomira, additional, Numanovic, Sanela, additional, Oggioni, Massimo, additional, Palminha, Paula, additional, Pariani, Elena, additional, Pellegrinelli, Laura, additional, Piralla, Antonio, additional, Pietsch, Corinna, additional, Piñeiro, Luis, additional, Rabella, Núria, additional, Rainetova, Petra, additional, Uceda Renteria, Sara Colonia, additional, Romero, María P, additional, Reynders, Marijke, additional, Roorda, Lieuwe, additional, Savolainen-Kopra, Carita, additional, Schuffenecker, Isabelle, additional, Soynova, Aysa, additional, Swanink, Caroline MA, additional, Ursic, Tina, additional, Verweij, Jaco J, additional, Vila, Jorgina, additional, Vuorinen, Tytti, additional, Simmonds, Peter, additional, Fischer, Thea K, additional, and Harvala, Heli, additional

Published
2021
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38. Enteroviruses in Respiratory Samples from Paediatric Patients of a Tertiary Care Hospital in Germany

Author

Baertl, Susanne, Pietsch, Corinna, Maier, Melanie, Hönemann, Mario, Bergs, Sandra, Liebert, Uwe G., Baertl, Susanne, Pietsch, Corinna, Maier, Melanie, Hönemann, Mario, Bergs, Sandra, and Liebert, Uwe G.

Abstract

Enteroviruses are associated with various diseases accompanied by rare but severe complications. In recent years, outbreaks of enterovirus D68 and enterovirus A71 associated with severe respiratory infections and neurological complications have been reported worldwide. Since information on molecular epidemiology in respiratory samples is still limited, the genetic diversity of enteroviruses was retrospectively analysed over a 4-year period (2013–2016) in respiratory samples from paediatric patients. Partial viral major capsid protein gene (VP1) sequences were determined for genotyping. Enteroviruses were detected in 255 (6.1%) of 4187 specimens. Phylogenetic analyses of 233 (91.4%) strains revealed 25 different genotypes distributed to Enterovirus A (39.1%), Enterovirus B (34.3%), and Enterovirus D (26.6%). The most frequently detected genotypes were enterovirus D68 (26.6%), coxsackievirus A6 (15.9%), and enterovirus A71 (7.3%). Enterovirus D68 detections were associated with lower respiratory tract infections and increased oxygen demand. Meningitis/encephalitis and other neurological symptoms were related to enterovirus A71, while coxsackievirus A6 was associated with upper respiratory diseases. Prematurity turned out as a potential risk factor for increased oxygen demand during enterovirus infections. The detailed analysis of epidemiological and clinical data contributes to the non-polio enterovirus surveillance in Europe and showed high and rapidly changing genetic diversity of circulating enteroviruses, including different enterovirus D68 variants.

Published
2021

40. Bewertungskriterien der Medienwahl im Coaching: Entwicklung und Einsatz eines Instrumentes zur Analyse von Akzeptanzkriterien für die Umsetzbarkeit zentraler Coach-Aktivitäten in ausgewählten Coachingmedien des Online- und Präsenz-Coachings aus der Sicht von Coaches

Author

Maier, Melanie

Subjects
Online-Coaching, Medienwahl, 300 Sozialwissenschaften, Soziologie, Coachingformate, Coaching, Präsenz-Coaching
Abstract

Coaching wird für unterschiedlichste Zielsetzungen und inhaltliche Schwerpunkte angeboten, um Menschen in der Bewältigung von Konflikten und bei ihrer Weiterentwicklung zu unterstützen. Auch die Vielfalt der angebotenen Formate, mit denen Coaching umgesetzt wird, nimmt beständig zu. Über das klassisch-bewährte Präsenz-Coaching hinaus werden zahlreiche Varianten von Online-Coaching, d.h. Coaching mit technischer Unterstützung, und auch Kombinationen aus Präsenz- und Online-Coaching offeriert. Empirisch unerforscht ist bisher allerdings, welche Beweggründe dafür verantwortlich sind, dass Coaches sich für das Angebot bestimmter Coachingformate entscheiden. Die vorliegende Arbeit beleuchtet daher die Bewertungskriterien von Coaches bei der Wahl von Coachingformaten und stellt drei Coachingformate in den Mittelpunkt: Präsenz-Coaching, Coaching über Online-Plattformen und Coaching per E-Mail. Das Forschungsdesign der Arbeit umfasst schwerpunktmäßig qualitative Ansätze, während diese um ausgewählte quantitative Erhebungen und Analysen ergänzt werden. Für die Befragung von 20 Coaches wurde das Instrument „Assessment Explorer für Coachingmedien“ (ASECOM) entwickelt, welches sich einerseits durch eine zielorientierte und forschungsökonomische Art der Erhebung und Auswertung der subjektiven Theorien von Befragten auszeichnet und darüber hinaus einen Reflexions- und Kalibrierungskreislauf zur Unterstützung der Meinungsbildung der Befragten beinhaltet. Um die Bewertungsprinzipien der Medienwahl fokussiert und in enger Verbindung mit der praktischen Arbeit von Coaches erkunden zu können, wurden in einer vorgelagerten Befragung von 32 Coaches drei zentrale Coach-Aktivitäten identifiziert, die den potenziell höchsten Zielerreichungsgrad im Coaching aufweisen. Anhand dieser drei zentralen Coach-Aktivitäten wurden anschließend die Umsetzbarkeitsbewertungen und dazugehörige gewichtete Bewertungskriterien der Coaches mithilfe des ASECOM in den drei ausgewählten Coachingformaten ermittelt. Hierbei zeigte sich, dass die Umsetzbarkeitsbewertung der zentralen Coach-Aktivitäten durch die Coaches in Abhängigkeit vom Coachingformat getroffen werden, was die Vermutung einer Wirkdimension der Medien im Coaching begründet. Weiterhin wurde festgestellt, dass die Prinzipien, die zur Bewertung von Coachingformaten herangezogen werden, nicht einheitlich eingesetzt, sondern von Coaches ebenfalls abhängig vom Coachingformat berücksichtigt werden.

Published
2020
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42. Suitcase Lab for Rapid Detection of SARS-CoV-2 Based on Recombinase Polymerase Amplification Assay

Author

El Wahed, Ahmed Abd, primary, Patel, Pranav, additional, Maier, Melanie, additional, Pietsch, Corinna, additional, Rüster, Dana, additional, Böhlken-Fascher, Susanne, additional, Kissenkötter, Jonas, additional, Behrmann, Ole, additional, Frimpong, Michael, additional, Diagne, Moussa Moïse, additional, Faye, Martin, additional, Dia, Ndongo, additional, Shalaby, Mohamed A., additional, Amer, Haitham, additional, Elgamal, Mahmoud, additional, Zaki, Ali, additional, Ismail, Ghada, additional, Kaiser, Marco, additional, Corman, Victor M., additional, Niedrig, Matthias, additional, Landt, Olfert, additional, Faye, Ousmane, additional, Sall, Amadou A., additional, Hufert, Frank T., additional, Truyen, Uwe, additional, Liebert, Uwe G., additional, and Weidmann, Manfred, additional

Published
2021
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46. Korrelation von okulomotorischen Veränderungen und mikrostrukturellen Läsionen bei Patienten mit M. Parkinson und anderen neurodegenerativen Parkinson-Syndromen

Author

Maier, Melanie, Kassubek, Jan, and Beer, Meinrad

Subjects
Steele-Richardson-Olszewski-Syndrom, Multisystematrophie, Parkinson disease, Eye movements, Electrooculography, Magnetic resonance imaging, Diffusionsgewichtete Magnetresonanztomografie, Progressive supranukleäre Blickparese, Parkinson-Krankheit, ddc:610, DDC 610 / Medicine & health, Videookulographie, Supranuclear palsy, Progressive
Abstract

Ein Merkmal neurodegenerativer Parkinson-Syndrome einschließlich des idiopathischen Parkinson-Syndroms (IPS), der Multisystematrophie (MSA) und der progressiven supranukleären Blickparese (PSP) sind charakteristisch beeinträchtigte Augenbewegungen. Mikrostrukturelle regionale Veränderungen, die mit der diffusionsgewichteten Sequenz des MRTs erfasst werden können, wurden bei Patienten mit Parkinsonismus bereits häufig beschrieben. Ziel der Studie war es, den Zusammenhang zwischen okulomotorischen Veränderungen und mikrostrukturellen Läsionen bei Patienten mit neurodegenerativen Parkinson-Syndromen zu untersuchen. Hierfür wurden bei 36 IPS-Patienten, 18 MSA-Patienten, 30 PSP-Patienten und 23 Kontrollpersonen eine videookulographische Untersuchung mit Überprüfung der glatten Folgebewegung (GFB) und visuell getriggerter Reaktiver Sakkaden (VGRS), sowie eine diffusionsgewichtete MRT-Sequenz zur Erstellung von Maps der fraktionellen Anisotropie (FA) durchgeführt und analysiert. Bei IPS-Patienten zeigten sich hypometrische Sakkaden und eine im Vergleich zu Kontrollen signifikant erhöhte Rate an sakkadischen Intrusionen (p

Published
2018
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