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Meiotic recombination forms crossovers important for proper chromosome segregation and offspring viability. This complex process involves many proteins acting at each of the multiple steps of recombination. Recombination initiates by formation of DNA double-strand breaks (DSBs), which in the several species examined occur with high frequency at special sites (DSB hotspots). In Schizosaccharomyces pombe, DSB hotspots are bound with high specificity and strongly activated by linear element (LinE) proteins Rec25, Rec27 and Mug20, which form colocalized nuclear foci with Rec10, essential for all DSB formation and recombination. Here, we test the hypothesis that the nuclear localization signal (NLS) of Rec10 is crucial for coordinated nuclear entry after forming a complex with other LinE proteins. In NLS mutants, all LinE proteins were abundant in the cytoplasm, not the nucleus; DSB formation and recombination were much reduced but not eliminated. Nuclear entry of limited amounts of Rec10, apparently small enough for passive nuclear entry, can account for residual recombination. LinE proteins are related to synaptonemal complex proteins of other species, suggesting that they also share an NLS, not yet identified, and undergo protein complex formation before nuclear entry. This article has an associated First Person interview with Mélody Wintrebert, joint first author of the paper.

Land and property price are determined by a number of characteristics, such as house-related characteristics and neighborhood-related characteristics. Accessibility to urban services and infrastructure is considered as an attribute group with a strong influence on this value. These impacts are diverse and complex, inconsistent, depending on each case study. The paper desires to use the linear regression method to evaluate the impact of accessibility to urban services and transportation on property prices in Hanoi. Some models were set up based on our assumptions on the relationship between accessibility-related attributes and estimated for 4 zones in the south of Hanoi and use them to compare the differences among these zones. Conclusions about the relationship between property prices and accessibility to transit, car-accessibility and accessibility to public amenities will help to enhance many ideas in managing land development in new centers established from Urban Mass Rapit Transit (UMRT) interchanges under the TOD concept - compact development, mixed land use, and easy accessibility.

In an attempt to identify predictors of long-term compliance with nasal continuous positive airway pressure (CPAP), we reviewed the records of 125 patients with obstructive sleep apnea (OSA) referred to our center for nasal CPAP trials. Severity of sleep apnea, sleep staging, daytime hypersomnolence, effectiveness of nasal CPAP, previous palatal surgery, and adverse reactions were compared in compliant and noncompliant patients. Nineteen patients did not tolerate a nasal CPAP trial in the laboratory or refused home nasal CPAP therapy. Ten patients were unavailable for follow-up. Of the remaining 96 patients, 23 (24 percent) had discontinued therapy, while 73 (76 percent) were still using nasal CPAP at 14.5 +/- 10.7 months (mean +/- SD). There were no statistically significant differences between the compliant and noncompliant patients in baseline apnea plus hypopnea index (AHI), baseline sleep staging, AHI while receiving nasal CPAP, sleep staging while receiving nasal CPAP, or frequency of adverse reactions during therapy. Severe daytime sleepiness was present in 65 of the 73 compliant patients and in 12 of the 23 noncompliant patients (p less than 0.05). Ten of 43 in the compliant group had previous palatal surgery compared with ten of 23 noncompliant patients (p less than 0.05). Our data confirm earlier observations in smaller samples that compliant and noncompliant patients have equally severe sleep apnea and good initial responses to nasal CPAP. Long-term compliance with nasal CPAP may be associated with the severity of daytime hypersomnolence on presentation. Previous palatal surgery was more frequent in patients who did not tolerate long-term nasal CPAP therapy.

A SPICE model of the foils and overbraiding of an integrated wiring system (IWS) harness is created to characterize their combined impedance as a function of frequency. Comparison of predicted values of impedance to measured data for a modeled cable harness is accomplished, in which fair agreement is seen to exist. A straightforward empirical adjustment is then made to the SPICE model, which results in bringing the model-predicted values into very close agreement with the measured data. Possible reasons for initial model-predicted value disagreement with empirical data are discussed, and form the basis for future research.