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To make a reliable diagnosis on haematological examination, it is necessary to examine further the morphology of the blood cells previously stained in the procedure for staining thin blood smears using a buffer solution with a standard pH of 6.4 to 6.8. One of the problems that may occur in the laboratory is that the buffer reagents are damaged, past the expiration date or running out, so alternative buffers are needed that are cheap, fast and easy to obtain. This study aims to evaluate the staining results from alternative buffers. Study used a quasi-experimental method, and using alternative buffers from bottled mineral water following SNI-01-0553 2006. The colour produced by some of these alternative buffers is almost equivalent to blood cells stained with phosphate buffer. The percentage of assessment results in the alternative buffer codes B, C, D, E and F compared with control (A) were 62.67%, 92.00%, 82.67%, 80.00% 88, 00%, and 68.00%. The use of alternative buffers for staining thin blood smears using the Giemsa, Wright stain, and Romanowsky method can be done with mineral water as an alternative buffer for sample codes C, D, E and F, while B and G cannot be used. This alternative buffer can be applied by laboratory personnel in urgent situations in limited equipment and material facilities.

Anak-anak merupakan penderita gizi buruk terbesar diseluruh dunia. Untuk mencapai sasaran Millenium Development Goals (MDG) yakni 15,5% angka prevalensi gizi buruk secara nasional harus diturunkan (Almawaddah,2016). Berdasarkan data Riskesda 2013, Kasus gizi buruk di Kalimantan Selatan adalah 8,2%, gizi kurang 19,2%. Angka tersebut menunjukkan kasus gizi buruk di Kalimantan Selatan masih tinggi, termasuk Kabupaten Batola. Data gizi buruk di Puskesmas Berangas Kecamatan Alalak Kabupaten Barito Kuala sampai dengan bulan Juni tahun 2016 adalah : gizi buruk 1 orang (0,045%), Balita BGM (bawah garis merah) 73 balita (3,3%) dan yang dirawat di TFC (Therapeutic Feeding Center) sebanyak 6 orang. Tujuan penelitian ini apakah ada pengaruh formula ikan haruan terhadap cita rasa dan asupan kalori serta protein pada anak penderita gizi buruk di Puskesmas Berangas Kabupaten Batola Kalsel Jenis penelitian yang dilakukan adalah penelitian eksperimen, penelitian ini menggunakan satu kelompok dengan dua kali perlakuan. Penelitian dilaksanakan di Puskesmas Berangas Kecamatan Alalak Kabupaten Barito Kuala Kalimantan Selatan. Subjek penelitian sebanyak 20 orang, dengan kriteria balita gizi buruk atau balita BGM yang 2 kali berturut-turut tidak naik timbangan berat badannya. Analisis yang menggunakan wilconxon test dengan taraf signifikansi yang digunakan adalah p

Latar Belakang: Penyebab utama kematian bayi baru lahir atau neonatal di dunia antara lain bayi lahir premature 29%, sepsis dan pneumonia 25% dan 23% merupakan bayi baru lahir dengan asfiksia dan trauma. Sepsis neonatorum merupakan salah satu penyakit infeksi yang dapat menyebabkan kematian pada bayi baru lahir, angka kejadian sepsis di Indonesia masih tinggi yaitu 8,7% sampai 30,29% dengan angka kematian 11,56% sampai 49,9%. Kejadian sepsis neonatorum di RSUD dr. H. Moch. Ansari Saleh pada tahun 2014 sebanyak 2,9%, tahun 2015 sebanyak 1,2%, dan tahun 2016 sebanyak 1,2%. Tujuan: Menganalisis faktor risiko ibu dalam meningkatkan kejadian sepsis neonatorum di RSUD Dr. H. Moch. Ansari Saleh Tahun 2017. Metode: Penelitian kuantitatif dengan rancangan case control . Populasi penelitian ini adalah semua bayi baru lahir yang tercatat di rekam medik RSUD dr. H. Moch Ansari Saleh tahun 2017 sebanyak 4516 orang. Sampel kasus diambil dengan total sampling sebanyak 55 orang sedangkan sampel kontrol diambil dengan teknik purposive sampling sebanyak 55 orang. Data dianalisis menggunakan uji chi-square. Hasil: Faktor risiko ibu yang terjadi selama kehamilan terbukti dapat meningkatkan kejadian sepsis neonatorum yaitu persalinan lama (ρ=0,032 < a=0,05), ketuban pecah dini (ρ=0,015 < a=0,05), dan usia kehamilan (ρ=0,022 < a=0,05). Dari ketiga faktor tersebut ibu yang mengalami ketuban pecah dini merupakan faktor yang paling berisiko menyebabkan sepsis neonatorum dibandingkan dengan yang tidak mengalami ketuban pecah dini (OR = 2,571) sedangkan faktor risiko ibu yang tidak berhubungan adalah infeksi antepartum dan komplikasi kehamilan. Simpulan: Dari lima faktor risiko ibu terdapat tiga faktor yang dapat meningkatkan kejadian sepsis neonatorum yaitu faktor yang paling beresiko adalah ketuban pecah dini dan faktor lainnya adalah persalinan lama dan usia kehamilan. Kata Kunci: Ketuban pecah dini, Persalinan lama, Sepsis neonatorum, Usia kehamilan Mother Risk Factors In Increasing The Incidence Of Neonatal Sepsis At Dr. H. Moch. Ansari Saleh Hospital Of Banjarmasin ABSTRACT Background: The main causes of newborn or neonatal deaths in the world include premature babies 29%, sepsis and pneumonia 25% and 23% are newborns with asphyxia and trauma. Neonatal sepsis is one of the infectious diseases that can cause death in newborns, the incidence of sepsis in Indonesia is still high at 8.7% to 30.29% with a mortality rate of 11.56% to 49.9% . The incidence of Neonatal Sepsis at Dr. H. Moch. Ansari Saleh Hospital of Banjarmasin in 2014 as much as 2.9%, in 2015 as many as 1.2%, and in 2016 as many as 1.2%. Objective: To analyzing maternal risk factors in increasing the incidence of neonatal sepsis at Dr. H. Moch. Ansari Saleh Hospital of Banjarmasin in 2017. Methods: Quantitative research with case control design. The study population was all newborns recorded in the medical record at Dr. H. Moch. Ansari Saleh Hospital of Banjarmasin in 2017 as many as 4516 people. Case samples were taken with a total sampling of 55 people while the control samples were taken with a purposive sampling technique of 55 people. Data were analyzed using the chi-square test . Results: Maternal risk factors that occur during pregnancy have been shown to increase the incidence of neonatal sepsis, pro long ed labor ( ρ =0,032 < a =0,05), premature rupture of membranes ( ρ =0,015 < a =0,05), and gestational age ( ρ =0,022 < a =0,05). Of the three factors, mothers who experienced premature rupture of membranes were the most at risk for causing neonatal sepsis compared with those who did not experience premature rupture of membranes (OR = 2,571) while unrelated maternal risk factors were antepartum infection and pregnancy complications . Conclusion: Of the five maternal risk factors there are three factors that can increase the incidence of neonatal sepsis, which is the most risky factor is premature rupture of the membranes and other factors are prolonged labor and gestational age . Key Words: G estational age , N eonatal sepsis , P remature rupture of membranes , Pro long ed labor

Context: Diarrhea characterized by a frequency increased of defecation more than 3 times/ day accompanied by changes in consistency (becoming liquid). The causes of diarrhea can be divided into 2 parts, which are direct causes and indirect causes that can facilitate or accelerate the occurrence of diarrhea, including bacteria, nutritional conditions, hygiene and sanitation, social culture such as population density, economic status, low birth weight, and immunization. Aims: The purpose of this study to examine the factors that influence the incidence of diarrhea. Methods: This research used secondary data, the prevalence of diarrhea and risk factors in Pasuruan Regency Health Center. Poisson regression approach with maximum likelihood estimator (MLE) estimation and Generalized Method Moment (GMM) used in this study. Results: The results showed that GMM estimation method in the Poisson regression model gave better performance in terms of significance parameters compared to the MLE method. Conclusions: Factors affecting the increase of diarrhea occurrences in area with an estimated MLE Percentage of non-exclusive breastfeeding and Percentage of normal nutritional status. Whereas the GMM estimation is the percentage of non-exclusive breastfeeding, the percentage of low birth weight, the percentage of population density, the percentage of smokers among family members in the house, the percentage of incomplete immunizations, the percentage of under-five years old children less than 2, the percentage of normal nutritional status, and the percentage of middle class socioeconomic status.

ACUTE (RTI) is still an important health problem because the cause of the death of infants and children under five high enough, 1 from 4 death that happens. The purpose of this research examines the factors that affect the genesis ACUTE (RTI) using poisson regression approach with estimates of the maximum likelihood estimator (MLE) and generalized method moment (GMM). This research done in the area of Health Clinic in South Kalimantan. The results of the study showed that the estimates of the GMM method on Poisson regression model gives better performance in terms of the significance of the parameters than the MLE method. The factors that affect an increasing number of the prevalence of ACUTE (RTI) a region namely persentase Breast Feeding non-exclusive (0.0279), the percentage of low birth weight (0.0569), the percentage of shelter density (0.028), the percentage of the existence of smoker family members in the house (0.0308), the percentage of immunization is not complete (0.0193). While the factors that affect a downturn in the number of the prevalence of ACUTE (RTI) in a region which is the percentage of the number of infants less than 2 (0.0364), the percentage of normal nutrition status (0.0224), the percentage of Mothers Education on high school (0.0339), and the percentage of social economy (UMP enough to top) (0.0194).