11 results on '"Mahidi Mardini"'
Search Results
2. Role of Androgens in Sex Differences in Cardiac Damage During Myocardial Infarction
- Author
-
John W. Funder, Thi Yen Loan Le, David J. Handelsman, Anastasia S. Mihailidou, Anthony W. Ashton, Mahidi Mardini, and Peter G. Stanton
- Subjects
Male ,medicine.medical_specialty ,medicine.drug_class ,Myocardial Infarction ,Down-Regulation ,Infarction ,Apoptosis ,Myocardial Reperfusion Injury ,Severity of Illness Index ,Rats, Sprague-Dawley ,Endocrinology ,Internal medicine ,medicine ,Animals ,Testosterone ,Myocardial infarction ,Sex Characteristics ,Estradiol ,business.industry ,Myocardium ,Dihydrotestosterone ,Estrogens ,Androgen ,medicine.disease ,Rats ,Androgen receptor ,Estrogen ,Androgens ,Female ,business ,Sex characteristics ,medicine.drug - Abstract
Age-specific incidence of ischemic heart disease in men is higher than in women, although women die more frequently without previous symptoms; the molecular mechanism(s) are poorly understood. Most studies focus on protection by estrogen, with less attention on androgen receptor-mediated androgen actions. Our aim was to determine the role of androgens in the sex differences in cardiac damage during myocardial infarction. Mature age-matched male and female Sprague Dawley rats, intact or surgically gonadectomized (Gx), received testosterone (T) or 17β-estradiol (E2) via subdermal SILASTIC (Dow Corning Corp.) implants; a subset of male rats received dihydrotestosterone. After 21 days, animals were anesthetized, and hearts were excised and subjected to ex vivo regional ischemia-reperfusion (I-R). Hearts from intact males had larger infarcts than those from females following I-R; Gx produced the opposite effect, confirming a role for sex steroids. In Gx males, androgens (dihydrotestosterone, T) and E2 aggravated I-R-induced cardiac damage, whereas in Gx females, T had no effect and E2 reduced infarct area. Increased circulating T levels up-regulated androgen receptor and receptor for advanced glycation end products, which resulted in enhanced apoptosis aggravating cardiac damage in both males and females. In conclusion, our study demonstrates, for the first time, that sex steroids regulate autophagy during myocardial infarction and shows that a novel mechanism of action for androgens during I-R is down-regulation of antiapoptotic protein Bcl-xL (B cell lymphoma-extra large), a key controller for cross talk between autophagy and apoptosis, shifting the balance toward apoptosis and leading to aggravated cardiac damage.
- Published
- 2014
3. Low-Dose Spironolactone Prevents Apoptosis Repressor With Caspase Recruitment Domain Degradation During Myocardial Infarction
- Author
-
Thi Yen Loan Le, Anastasia S. Mihailidou, Viive M. Howell, Anthony W. Ashton, John W. Funder, and Mahidi Mardini
- Subjects
Male ,medicine.medical_specialty ,Immunoblotting ,Myocardial Infarction ,Muscle Proteins ,Repressor ,Apoptosis ,In Vitro Techniques ,Spironolactone ,Caspase 8 ,Cell Line ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Mineralocorticoid receptor ,Internal medicine ,Internal Medicine ,medicine ,Animals ,Caspase ,Mineralocorticoid Receptor Antagonists ,Aldosterone ,Dose-Response Relationship, Drug ,biology ,Caspase 3 ,Reverse Transcriptase Polymerase Chain Reaction ,Caspase 2 ,Caspase 9 ,Eplerenone ,Rats ,Enzyme Activation ,Receptors, Mineralocorticoid ,Endocrinology ,chemistry ,Proteolysis ,biology.protein ,RNA Interference ,Apoptosis Regulatory Proteins ,medicine.drug - Abstract
Low-dose mineralocorticoid receptor antagonists reduce morbidity and mortality in patients with heart failure and myocardial infarction, despite normal plasma aldosterone levels. Since apoptosis plays an important role in heart failure and postinfarction left ventricular remodeling, we examined whether low-dose mineralocorticoid receptor antagonists modulate cardiomyocyte death by regulating the apoptosis repressor protein apoptosis repressor with caspase recruitment domain to lessen the extent of apoptosis. Hearts from adult male Sprague-Dawley rats were subjected to regional ischemia followed by reperfusion ex vivo, with mineralocorticoid receptor antagonists added to perfusates before ischemia. Low-dose spironolactone (10 nmol/L) or eplerenone (100 nmol/L) significantly reduced infarct size. Spironolactone also prevented cleavage of the apoptotic chromatin condensation inducer in the nucleus and of the inhibitor of caspase-activated DNAse induced by ischemia-reperfusion, thereby abolishing chromatin condensation and internucleosomal cleavage. Ischemia-reperfusion–induced activation of caspases 2, 3, and 9, but not caspase 8, was prevented by spironolactone, suggesting targeted regulation of the intrinsic pathway. Low-dose spironolactone and eplerenone prevented loss of the apoptosis repressor with the caspase recruitment domain and reduced myocyte death. In H9c2 cells, mineralocorticoid receptor activation by aldosterone resulted in apoptosis repressor with caspase recruitment domain degradation and enhanced apoptosis; these actions were prevented by coadministration of spironolactone. Using a triple lysine mutant we identified that aldosterone enhances posttranscriptional degradation of the apoptosis repressor with a caspase recruitment domain via the ubiquitin-proteasomal pathway. Our data demonstrate that low-dose mineralocorticoid receptor antagonists reduce infarct size and apoptosis in the reperfused myocardium by preventing the apoptosis repressor with caspase recruitment domain degradation.
- Published
- 2012
4. High-dose insulin in experimental myocardial infarction in rabbits: protection against effects of hyperglycaemia
- Author
-
Mahidi Mardini, Vincent W. Wong, Anastasia S. Mihailidou, and N. Wah Cheung
- Subjects
Blood Glucose ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Myocardial Infarction ,Ischemia ,Apoptosis ,High dose insulin ,Endocrinology ,Diabetes mellitus ,Internal medicine ,Internal Medicine ,medicine ,Animals ,Insulin ,Myocytes, Cardiac ,cardiovascular diseases ,Myocardial infarction ,Cells, Cultured ,Cardioprotection ,Dose-Response Relationship, Drug ,business.industry ,medicine.disease ,Infarct size ,Disease Models, Animal ,Cytoprotection ,Hyperglycemia ,Cardiology ,Rabbits ,business - Abstract
Hyperglycaemia at the time of acute myocardial infarction (AMI) is a predictor of survival and is associated with increased mortality and morbidity in patients with or without diabetes mellitus. On the other hand, insulin has been shown to reduce myocardial injury in experimental studies but its benefits have not been confirmed in clinical studies.The isolated perfused heart model was used to examine the direct effect of incremental doses of insulin and varying degrees of hyperglycaemia on infarct size and cardiomyocyte apoptosis in rabbit hearts. The rabbit hearts were subjected to 30-min ischaemia and 2.5-h reperfusion.Insulin, given alone just before reperfusion, dramatically reduced infarct size in a dose-dependent manner (75-300 μU/ml) during experimental myocardial infarction (46%±2% to 10.9%±3%, P.001). Acutely elevated glucose levels (33 mmol/L) induced a significantly greater infarct size and cardiomyocyte apoptosis compared to hearts subjected to normal glucose levels. On the other hand, high-dose insulin (300 μU/ml) given 5 min before reperfusion attenuated the extent of infarction and reduced apoptosis in hearts that were exposed to high glucose levels.Acutely elevated levels of glucose induced larger infarct area during ischaemia-reperfusion, and this is mediated through proapoptotic pathways. Insulin, when given just before reperfusion, confers cardioprotection in a dose-dependent manner and reverses the detrimental effect of acute hyperglycaemia. High-dose insulin as well as maintaining normoglycaemia remain important factors that improve outcomes following myocardial infarction.
- Published
- 2011
5. Glucocorticoids Activate Cardiac Mineralocorticoid Receptors During Experimental Myocardial Infarction
- Author
-
Mahidi Mardini, Thi Yen Loan Le, John W. Funder, and Anastasia S. Mihailidou
- Subjects
Male ,Agonist ,medicine.medical_specialty ,Hydrocortisone ,medicine.drug_class ,Myocardial Infarction ,Apoptosis ,Myocardial Reperfusion Injury ,Spironolactone ,Dexamethasone ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Hormone Antagonists ,Glucocorticoid receptor ,Mineralocorticoid receptor ,Internal medicine ,Internal Medicine ,medicine ,Animals ,Angina, Unstable ,Aldosterone ,Glucocorticoids ,Mineralocorticoid Receptor Antagonists ,business.industry ,Mifepristone ,Rats ,Receptors, Mineralocorticoid ,Endocrinology ,chemistry ,Mineralocorticoid ,business ,hormones, hormone substitutes, and hormone antagonists ,Glucocorticoid ,medicine.drug - Abstract
Myocardial ischemia-reperfusion leads to significant changes in redox state, decreased postischemic functional recovery, and cardiomyocyte apoptosis, with development and progression of heart failure. Ischemia-reperfusion in the isolated perfused rat heart has been used as a model of heart failure. Clinically, mineralocorticoid receptor blockade in heart failure decreases morbidity and mortality versus standard care alone. The effects of corticosteroids on infarct area and apoptosis were determined in rat hearts subjected to 30 minutes of ischemia and 2.5 hours of reperfusion. Both aldosterone and cortisol increased infarct area and apoptotic index, an effect half-maximal between 1 and 10 nM and reversed by spironolactone. Dexamethasone and mifepristone aggravated infarct area and apoptotic index, similarly reversed by spironolactone. Spironolactone alone reduced infarct area and apoptotic index below ischemia-reperfusion alone, in hearts from both intact and adrenalectomized rats. The present study shows that cardiac damage is aggravated by activation of mineralocorticoid receptors by aldosterone or cortisol or of glucocorticoid receptors by dexamethasone. Mifepristone unexpectedly acted as a glucocorticoid receptor agonist, for which there are several precedents. Spironolactone protected cardiomyocytes via inverse agonist activity at mineralocorticoid receptors, an effect near maximal at a relatively low dose (10 nM). Spironolactone acts not merely by excluding corticosteroids from mineralocorticoid receptors but as a protective inverse agonist at low concentration. Mineralocorticoid receptor antagonists may, thus, provide an additional therapeutic advantage in unstable angina and acute myocardial infarction.
- Published
- 2009
6. Hyperaldosteronemia in Rabbits Inhibits the Cardiac Sarcolemmal Na + -K + Pump
- Author
-
Peter S. Hansen, Keld Kjeldsen, Helge H. Rasmussen, Mahidi Mardini, Anastasia S. Mihailidou, and Henning Bundgaard
- Subjects
Male ,medicine.medical_specialty ,Patch-Clamp Techniques ,Physiology ,medicine.drug_class ,Sodium ,chemistry.chemical_element ,Spironolactone ,chemistry.chemical_compound ,Sarcolemma ,Mineralocorticoid receptor ,TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY ,Internal medicine ,Hyperaldosteronism ,medicine ,Animals ,Myocyte ,Patch clamp ,Hyperaldosteronemia ,Na+/K+-ATPase ,Ouabain ,Aldosterone ,Mineralocorticoid Receptor Antagonists ,4-Nitrophenylphosphatase ,Myocardium ,Osmolar Concentration ,Electric Conductivity ,Intracellular Membranes ,Papillary Muscles ,Endocrinology ,chemistry ,Mineralocorticoid ,Potassium ,Rabbits ,Sodium-Potassium-Exchanging ATPase ,Cardiology and Cardiovascular Medicine - Abstract
Abstract —Aldosterone upregulates the Na + -K + pump in kidney and colon, classical target organs for the hormone. An effect on pump function in the heart is not firmly established. Because the myocardium contains mineralocorticoid receptors, we examined whether aldosterone has an effect on Na + -K + pump function in cardiac myocytes. Myocytes were isolated from rabbits given aldosterone via osmotic minipumps and from controls. Electrogenic Na + -K + pump current, arising from the 3:2 Na + :K + exchange ratio, was measured in single myocytes using the whole-cell patch clamp technique. Treatment with aldosterone induced a decrease in pump current measured when myocytes were dialyzed with patch pipette solution containing Na + in a concentration of 10 mmol/L, whereas there was no effect measured when the solution contained 80 mmol/L Na + . Aldosterone had no effect on myocardial Na + -K + pump concentration evaluated by vanadate-facilitated [ 3 H]ouabain binding or by K + -dependent paranitrophenylphosphatase activity in crude homogenates. Aldosterone induced an increase in intracellular Na + activity. The aldosterone-induced decrease in pump current and increased intracellular Na + were prevented by cotreatment with the mineralocorticoid receptor antagonist spironolactone. Our results indicate that hyperaldosteronemia decreases the apparent Na + affinity of the Na + -K + pump, whereas it has no effect on maximal pump capacity.
- Published
- 2000
7. Spironolactone Restores Redox Balance during Myocardial Ischemia-Reperfusion
- Author
-
Thi Yen Loan Le, Anthony Ashton, Mahidi Mardini, and Anastasia Mihailidou
- Published
- 2011
8. Factors affecting diurnal variability of ventricular tachyarrhythmias detected by multiprogrammable implantable cardioverter-defibrillators
- Author
-
Astin K.Y. Lee, R. Denniss, Mahidi Mardini, and David L. Ross
- Subjects
Pulmonary and Respiratory Medicine ,Tachycardia ,medicine.medical_specialty ,Ejection fraction ,business.industry ,Ventricular Tachyarrhythmias ,Amiodarone ,Endocrinology ,Internal medicine ,cardiovascular system ,Cardiology ,Medicine ,In patient ,cardiovascular diseases ,Circadian rhythm ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Morning ,medicine.drug - Abstract
Many cardiovascular events, including ventricular arrhythmias, display diurnal variability with a morning peak, and a less pronounced afternoon peak. Since the advent of multiprogrammable implantable cardioverter-defibrillators (ICDs), it has been possible to analyse ventricular tachyarrhythmic events. This study aims to evaluate the circadian pattern of ventricular tachycardias in patients treated with ICDs and examines whether antiarrhythmic medications affect this pattern. Data recorded from 83 patients' ICDs were manually analysed and events other than ventricular arrhythmias were excluded. There was a morning peak of ventricular arrhythmias at around 9.00 a.m. This peak was maintained in patients with ejection fractions of less than 40% and those whose arrhythmias had cycle lengths of less than 230ms. Beta blockers appeared to have no effect on this morning peak but the peak appeared later with amiodarone.
- Published
- 2005
9. Rapid, nongenomic effects of aldosterone in the heart mediated by epsilon protein kinase C
- Author
-
Anastasia S. Mihailidou, Mahidi Mardini, and John W. Funder
- Subjects
Male ,medicine.medical_specialty ,Patch-Clamp Techniques ,medicine.drug_class ,Sodium-Potassium-Chloride Symporters ,medicine.medical_treatment ,Heart Ventricles ,Protein Kinase C-epsilon ,Biology ,chemistry.chemical_compound ,Endocrinology ,Mineralocorticoid receptor ,Internal medicine ,medicine ,Animals ,Ventricular Function ,Patch clamp ,Enzyme Inhibitors ,Aldosterone ,Protein kinase C ,Protein Kinase C ,Muscle Cells ,Activator (genetics) ,Electric Conductivity ,Heart ,Steroid hormone ,chemistry ,Mineralocorticoid ,Rabbits ,Sodium-Potassium-Exchanging ATPase ,Intracellular - Abstract
Aldosterone elevates Na+/K+/2Cl− cotransporter activity in rabbit cardiomyocytes within 15 min, an effect blocked by K-canrenoate and thus putatively mineralocorticoid receptor mediated. Increased cotransporter activity raises intracellular [Na+] sufficient to produce a secondary increase in Na+-K+ pump activity; when this increase in intracellular [Na+] is prevented, a rapid effect of aldosterone to lower pump activity is seen. Addition of transcription inhibitor actinomycin D did not change basal or aldosterone-induced lowered pump activity, indicating a direct, nongenomic action of aldosterone. We examined a possible role for protein kinase C (PKC) in the rapid nongenomic effects of aldosterone. Single ventricular myocytes and pipette solutions containing 10 mm intracellular [Na+] were used in patch clamp studies to measure Na+-K+ pump activity. Aldosterone lowered pump current, an effect abolished by ε PKC (εPKC) inhibition but neither αPKC nor scrambled εPKC; addition of εPKC activator peptide mimicked the rapid aldosterone effect. In rabbits chronically infused with aldosterone, the lowered pump current in cardiomyocytes was acutely (≤15 min) restored by εPKC inhibition. These studies show that rapid effects of aldosterone on Na+-K+ pump activity are nongenomic and specifically εPKC mediated; in addition, such effects may be prolonged (7 d) and long-lived (∼4 h isolated cardiomyocyte preparation time). The rapid, prolonged, long-lived effects can be rapidly (≤15 min) reversed by εPKC blockade, suggesting a hitherto unrecognized complexity of aldosterone action in the heart and perhaps by extension other tissues.
- Published
- 2003
10. Mineralocorticoid and angiotensin receptor antagonism during hyperaldosteronemia
- Author
-
Matthew Raison, Mahidi Mardini, Anastasia S. Mihailidou, and John W. Funder
- Subjects
Male ,medicine.medical_specialty ,Patch-Clamp Techniques ,medicine.drug_class ,Heart Ventricles ,Blood Pressure ,Pharmacology ,Spironolactone ,Losartan ,Membrane Potentials ,chemistry.chemical_compound ,Angiotensin Receptor Antagonists ,Mineralocorticoid receptor ,Internal medicine ,Hyperaldosteronism ,Internal Medicine ,medicine ,Animals ,Ventricular Function ,Magnesium ,Na+/K+-ATPase ,Hyperaldosteronemia ,Aldosterone ,Mineralocorticoid Receptor Antagonists ,Chemistry ,Sodium ,Eplerenone ,Endocrinology ,Mineralocorticoid ,Potassium ,Rabbits ,Sodium-Potassium-Exchanging ATPase ,medicine.drug - Abstract
Elevated aldosterone levels induce a spironolactone-inhibitable decrease in cardiac sarcolemmal Na + -K + pump function. Because pump inhibition has been shown to contribute to myocyte hypertrophy, restoration of Na + -K + pump function may represent a possible mechanism for the cardioprotective action of mineralocorticoid receptor (MR) blockade. The present study examines whether treatment with the angiotensin type 1 receptor antagonist losartan, with either spironolactone or eplerenone, has additive effects on sarcolemmal Na + -K + pump activity in hyperaldosteronemia. New Zealand White rabbits were divided into 7 different groups: controls, aldosterone alone, aldosterone plus spironolactone, aldosterone plus eplerenone, aldosterone plus losartan, aldosterone plus losartan and spironolactone, and aldosterone plus losartan and eplerenone. After 7 days, myocytes were isolated by enzymatic digestion. Electrogenic Na + -K + pump current (I p ), arising from the 3:2 Na + :K + exchange ratio, was measured by the whole-cell patch clamp technique. Elevated aldosterone levels lowered I p ; treatment with losartan reversed aldosterone-induced reduced pump function, as did MR blockade. Coadministration of spironolactone or eplerenone with losartan enhanced the losartan effect on pump function to a level similar to that measured in rabbits given losartan alone in the absence of hyperaldosteronemia. In conclusion, hyperaldosteronemia induces a decrease in I p at near physiological levels of intracellular Na + concentration. Treatment with losartan reverses this aldosterone-induced decrease in pump function, and coadministration with MR antagonists produces an additive effect on pump function, consistent with a beneficial effect of MR blockade in patients with hypertension and congestive heart failure treated with angiotensin type 1 receptor antagonists.
- Published
- 2002
11. Renin angiotension aldosterone antagonists ameliorate cyclosporine induced toxicity
- Author
-
Mahidi Mardini, Taflen Fraser, Helege Rassussen, and Anastasia S. Mihailidou
- Subjects
Pulmonary and Respiratory Medicine ,chemistry.chemical_compound ,Aldosterone ,chemistry ,business.industry ,Renin–angiotensin system ,Toxicity ,Medicine ,Pharmacology ,Cardiology and Cardiovascular Medicine ,business - Published
- 2003
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.