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Permanent lateral incisors have been considered as major missing teeth in the mouth. The main aim ofthe present study is to conduct a comparative study of maxillary and mandibular arch widths, overjet andoverbite for a sample with missing lateral incisors and normal class I occlusion.The sample of the study comprises sixty pairs of study models of both genders, which were separated intotwo groups. The first group consisted of thirty casts of bilateral congenital missing lateral incisors, while thesecond group consisted of thirty casts of the class I occlusion. The intercanine width, interpremolar width,intermolar width, overjet and overbite were measured for the sixty samples.The results of this study demonstrate a significant reduction in maxillary and mandibular arch widthin bilateral missing lateral Incisors as compared with normal class I group. The results also revealed asignificant reduction in overjet and overbite in bilateral missing lateral incisors as compared with normalclass I group. A significant difference was also reported between the two groups based on gender differencein the maxillary arch width. Conversely, non-significant difference was found in the mandibular arch width.The present study also shows non-significant difference based on gender difference in overjet and overbitein the two groups.