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39 results on '"Maeta, Noritaka"'

1. Evaluation of Skin Wound Healing with Biosheets Containing Somatic Stem Cells in a Dog Model: A Pilot Study.

Author

Maeta, Noritaka, Iwai, Ryosuke, Takemitsu, Hiroshi, Akashi, Natsuki, Miyabe, Masahiro, Funayama-Iwai, Marina, and Nakayama, Yasuhide

Subjects
*SKIN regeneration, *SOMATIC cells, *STEM cells, *HEALING, *WOUND healing, *MESENCHYMAL stem cells, *DOGS, *BEAGLE (Dog breed)
Abstract

The administration of mesenchymal stem cells (MSCs) has a positive effect on wound healing; however, the lack of adequate MSC engraftment at the wound site is a major limiting factor in current MSC-based therapies. In this study, a biosheet prepared using in-body tissue architecture (iBTA) was used as a material to address these problems. This study aimed to assess and evaluate whether biosheets containing somatic stem cells would affect the wound healing process in dogs. Biosheets were prepared by subcutaneously embedding molds in beagles. These were then evaluated grossly and histologically, and the mRNA expression of inflammatory cytokines, interleukins, and Nanog was examined in some biosheets. Skin defects were created on the skin of the beagles to which the biosheets were applied. The wound healing processes of the biosheet and control (no biosheet application) groups were compared for 8 weeks. Nanog mRNA was expressed in the biosheets, and SSEA4/CD105 positive cells were observed histologically. Although the wound contraction rates differed significantly in the first week, the biosheet group tended to heal faster than the control group. This study revealed that biosheets containing somatic stem cells may have a positive effect on wound healing. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Expression profile of immunoregulatory factors in canine tumors

Author

Murakami, Kohei, primary, Miyatake, Saki, additional, Miyamae, Jiro, additional, Saeki, Kanna, additional, Shinya, Mizutani, additional, Akashi, Natsuki, additional, Mitsui, Ikki, additional, Kobayashi, Kosuke, additional, Saeki, Kohei, additional, Maeta, Noritaka, additional, Kanda, Teppei, additional, Okamura, Yasuhiko, additional, and Hemmi, Hiroaki, additional

Published
2022
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27. Effect of intramuscular administration of medetomidine and xylazine on tear flow measured by the Schirmer tear test I in healthy cats.

Author

Kanda, Teppei, Shimizu, Yuki, Hanazono, Chisa, Maki, Saya, Maeta, Noritaka, Itoi, Takamasa, Furumoto, Kayo, Okamura, Yasuhiko, Itoh, Yoshiki, and Furukawa, Toshinori

Abstract

Objectives: This study aimed to investigate the effects of intramuscular medetomidine and xylazine on tear flow in healthy cats. Methods: Five cats each received medetomidine 10, 20, 40 and 80 µg/kg IM; xylazine 1.0, 2.0, 4.0 and 8.0 mg/kg IM; and physiological saline (2.0 ml IM) in a randomised order separated by intervals of at least 1 week. The Schirmer tear test (STT) I was performed in both eyes before and 0.25, 0.5, 0.75, 1, 2, 3, 4, 5, 6, 7, 8 and 24 h after each dose. Results: The STT I value decreased significantly at 0.5 and 1.0 h and at 0.75 and 1.0 h in both eyes after administration of medetomidine at 10 or 40 µg/kg. After administration of medetomidine 80 µg/kg, there was a significant decrease in the STT I reading at 0.75, 2 and 3 h in the left eye and 0.75, 1, 2 and 3 h in the right eye. The STT I value decreased significantly at: 0.5, 0.75, 1 and 2 h in the left eye and 0.75 h in the right eye after administration of xylazine 1.0 mg/kg; 0.5, 0.75, 1 and 2 h in the left eye and 0.5, 0.75, 1 and 3 h in the right eye after administration of xylazine 2.0 mg/kg; 0.5, 0.75, 1 and 2 h in both eyes after administration of xylazine 4.0 mg/kg; and 0.5, 0.75, 1, 2 and 3 h in the left eye and 0.75, 1, 2, 3 and 4 h in the right eye after administration of xylazine 8.0 mg/kg. Conclusions and relevance: Both medetomidine and xylazine significantly decreased feline tear flow measured by STT I. Therefore, the ocular surface should be monitored carefully and protected appropriately in cats treated with these sedatives. [ABSTRACT FROM AUTHOR]

Published
2019
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38. Establishment of a canine lens epithelial cell line.

Author

Matsuda A, Shimizu Y, Kanda T, Ohnishi A, Maeta N, Miyabe M, Saeki K, and Itoh Y

Subjects
Animals, Cell Line, Dogs, Epithelial Cells physiology, Lens, Crystalline cytology
Abstract

Some immortalized lens epithelial cell lines have been established and are useful for molecular analysis. The establishment of additional cell lines must, however, enable a variety of in-vitro examinations. The objective of this study was to establish a new canine lens epithelial cell line by isolating CLC-1 cells from the lens tissue of a dog with cataracts. In CLC-1 cells, transforming growth factor beta (TGF-β) treatment significantly decreased gene expression of an epithelial marker and elevated that of mesenchymal markers; these characteristics are similar to those of a human lens epithelial cell line. Interestingly, CLC-1 cells exhibited lower expression of an epithelial marker and higher expression of mesenchymal markers than an anterior lens capsule. These results suggest that CLC-1 cells were derived from a cell population that was committed to epithelial-mesenchymal transition in cataract lens tissue. In conclusion, CLC-1 cells could be useful for analyzing molecular pathogenesis in canine cataracts., (Copyright and/or publishing rights held by the Canadian Veterinary Medical Association.)

Published
2021

39. Comparative analysis of canine mesenchymal stem cells and bone marrow-derived mononuclear cells.

Author

Maeta N, Tamura K, Ezuka F, and Takemitsu H

Abstract

Background and Aim: Mesenchymal stem cells (MSCs), which have multi-lineage differentiation potentials, are a promising source for regenerative medicine. However, the focus of study of MSCs is shifting from the characterization of the differentiation potential to their secretion potential for cell transplantation. Tissue regeneration and the attenuation of immune responses are thought to be affected by the secretion of multiple growth factors and cytokines by MSCs. However, the secretion potential of MSCs profiling remains incompletely characterized. In this study, we focused on the secretion ability related and protein mRNA expression of dog adipose tissue-derived MSCs (AT-MSC), bone marrow (BM)-derived MSCs, and BM-derived mononuclear cells (BM-MNC)., Materials and Methods: Real-time polymerase chain reaction analyses revealed mRNA expression of nine growth factors and seven interleukins in these types of cells and three growth factors protein expression were determined using Enzyme-linked immunosorbent assay., Results: For the BM-MNC growth factors, the mRNA expression of transforming growth factor-β (TGF-β) was the highest. For the BM-derived MSC (BM-MSC) and AT-MSC growth factors, the mRNA expression of vascular endothelial growth factor (VEGF) was highest. BM-MSCs and AT-MSCs showed similar expression profiles. In contrast, BM-MNCs showed unique expression profiles for hepatocyte growth factor and epidermal growth factor. The three types of cells showed a similar expression of TGF-β., Conclusion: We conclude that expression of cytokine proteins and mRNAs suggests involvement in tissue repair and protection., (Copyright: © Maeta, et al.)

Published
2021
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