1. Genome-wide gene expression profiling suggests distinct radiation susceptibilities in sporadic and post-Chernobyl papillary thyroid cancers.
- Author
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Detours, V, Delys, L, Libert, F, Weiss Solís, D, Bogdanova, T, Dumont, JE, Franc, B, Thomas, G, and Maenhaut, C
- Subjects
Lymphocytes ,Humans ,Carcinoma ,Papillary ,Thyroid Neoplasms ,Radiation Injuries ,Genetic Predisposition to Disease ,Hydrogen Peroxide ,Microarray Analysis ,Gene Expression Profiling ,Transcription ,Genetic ,Gene Expression Regulation ,Neoplastic ,Gamma Rays ,Chernobyl Nuclear Accident ,Adolescent ,Adult ,Aged ,Middle Aged ,Child ,Child ,Preschool ,Infant ,Tissue Banks ,France ,Female ,Male ,Carcinoma ,Papillary ,Preschool ,Gene Expression Regulation ,Neoplastic ,Transcription ,Genetic ,Oncology and Carcinogenesis ,Public Health and Health Services ,Oncology & Carcinogenesis - Abstract
Papillary thyroid cancers (PTCs) incidence dramatically increased in the vicinity of Chernobyl. The cancer-initiating role of radiation elsewhere is debated. Therefore, we searched for a signature distinguishing radio-induced from sporadic cancers. Using microarrays, we compared the expression profiles of PTCs from the Chernobyl Tissue Bank (CTB, n=12) and from French patients with no history of exposure to ionising radiations (n=14). We also compared the transcriptional responses of human lymphocytes to the presumed aetiological agents initiating these tumours, gamma-radiation and H(2)O(2). On a global scale, the transcriptomes of CTB and French tumours are indistinguishable, and the transcriptional responses to gamma-radiation and H(2)O(2) are similar. On a finer scale, a 118 genes signature discriminated the gamma-radiation and H(2)O(2) responses. This signature could be used to classify the tumours as CTB or French with an error of 15-27%. Similar results were obtained with an independent signature of 13 genes involved in homologous recombination. Although sporadic and radio-induced PTCs represent the same disease, they are distinguishable with molecular signatures reflecting specific responses to gamma-radiation and H(2)O(2). These signatures in PTCs could reflect the susceptibility profiles of the patients, suggesting the feasibility of a radiation susceptibility test.
- Published
- 2007