1. Fimbriae and lipopolysaccharides are necessary for co-aggregation between Lactobacilli and Escherichia coli
- Author
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Yasushi Morinaga, Kouhei Mizuno, Madoka Ishiba, Hirokazu Ogihara, Yumi Usui, and Soichi Furukawa
- Subjects
Lipopolysaccharides ,Deletion mutant ,Lipopolysaccharide ,medicine.medical_treatment ,Fimbria ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Biochemistry ,Analytical Chemistry ,Microbiology ,chemistry.chemical_compound ,Lactobacillus ,Escherichia coli ,medicine ,Sodium dodecyl sulfate ,Molecular Biology ,Cell Aggregation ,Protease ,biology ,Organic Chemistry ,Sodium Dodecyl Sulfate ,food and beverages ,General Medicine ,biology.organism_classification ,Coculture Techniques ,chemistry ,Co aggregation ,Fimbriae Proteins ,Biotechnology - Abstract
Cells of Lactobacilli co-aggregated with Escherichia coli K-12 cells to form co-aggregates under mixed-culture conditions at 37 °C for 24 h. Co-aggregation was inhibited by sodium dodecyl sulfate but not by protease. E. coli deletion mutants of fimbriae formation and lipopolysaccharide (LPS) formation did not co-aggregate with Lactobacilli. These results showed that fimbriae and LPS are necessary for co-aggregation between Lactobacilli and E. coli.
- Published
- 2014
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