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3 results on '"Maddy Duhamel"'

1. The development of specific rRNA-derived oligonucleotide probes for Haemophilus ducreyi, the causative agent of chancroid

Author

Maddy Duhamel, Hugo Van Heuverswyn, Jean Luc Decourt, Rudi Rossau, and Geert Jannes

Subjects
Molecular Sequence Data, Biology, Polymerase Chain Reaction, Microbiology, Chancroid, Haemophilus ducreyi, Cistron, 23S ribosomal RNA, RNA, Ribosomal, 16S, Humans, Cloning, Molecular, Genetics, Base Sequence, Oligonucleotide, Nucleic acid sequence, Nucleic Acid Hybridization, Ribosomal RNA, biology.organism_classification, 16S ribosomal RNA, Molecular biology, RNA, Bacterial, RNA, Ribosomal, 23S, Nucleic Acid Conformation, Oligonucleotide Probes, Molecular probe, Sequence Alignment
Abstract

Summary Part of a ribosomal ribonucleic acid (rRNA) cistron of Haemophilus ducreyi was enzymically amplified using conserved primers within the rRNA molecules, cloned in a plasmid vector, and sequenced. From the nucleotide sequence, eight oligonucleotides complementary to different regions in the 16S and 23S rRNA molecules were selected, chemically synthesized, and used as hybridization probes. Hybridization experiments with at least 41 H. ducreyi strains and 13 or 14 non-H. ducreyi strains revealed that all eight oligonucleotide probes were highly reliable and completely specific for H. ducreyi strains. Comparisons of 16S rRNA sequences confirm that H. ducreyi is a member of the Pasteurellaceae though not closely related to other species in this family.

Published
1991
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2. DNA probes for Bordetella species and a colorimetric reverse hybridization assay for the detection of Bordetella pertussis

Author

Anne Michielsen, Geert Jannes, Rudi Rossau, Karel Kersters, Hugo Van Heuverswyn, and Maddy Duhamel

Subjects
DNA, Bacterial, Bordetella pertussis, Bordetella, Molecular Sequence Data, Polymerase Chain Reaction, Sensitivity and Specificity, chemistry.chemical_compound, RNA, Ribosomal, 16S, Molecular Biology, biology, Base Sequence, Hybridization probe, Nucleic Acid Hybridization, Cell Biology, Ribosomal RNA, biology.organism_classification, Molecular biology, RNA, Bacterial, RNA, Ribosomal, 23S, chemistry, Agarose gel electrophoresis, Oligomer restriction, Molecular probe, DNA Probes, DNA
Abstract

Three oligonucleotide probe sequences were inferred from the 16S ribosomal ribonucleic acid (rRNA) and the 16S–23S rRNA spacer sequences of Bordetella pertussis ATCC 10380. These probes were used in hybridization tests with deoxyribonucleic acid from Bordetella species and other relevant bacterial taxa. A probe from the spacer region hybridized exclusively to the B. pertussis strains tested and not to strains from other species. Using a combination of three probes, B. pertussis B. parapertussis/B. bronchiseptica and B. avium could be specifically identified and differentiated from other taxa. Differentiation between B. parapertussis and B. bronchiseptica was not possible with the probes used. Using the spacer probe, a colorimetric hybridization assay specific for B. pertussis was developed based on enzymatic amplification of the 16S–23S rRNA spacer and reverse hybridization in microtitre wells. As compared with results using agarose gel electrophoresis, and Southern and dot-spot hybridization with a 32P-labelled probe, this assay proved to be faster and easier to perform and was found to be at least as sensitive and specific.

Published
1992

3. Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae

Author

Maddy Duhamel, H. Van Heuverswyn, Peter Piot, Rudi Rossau, and E Van Dyck

Subjects
Microbiology (medical), Molecular Probe Techniques, Neisseria meningitidis, Biology, urologic and male genital diseases, medicine.disease_cause, Microbiology, Gonorrhea, Plasmid, Species Specificity, medicine, Humans, Hybridization probe, Ribosomal RNA, biology.organism_classification, Molecular biology, Neisseria gonorrhoeae, female genital diseases and pregnancy complications, RNA, Bacterial, Evaluation Studies as Topic, RNA, Ribosomal, Neisseriaceae, DNA Probes, Molecular probe, Oligomer restriction, Plasmids, Research Article
Abstract

The reliability of an rRNA-derived oligonucleotide probe for Neisseria gonorrhoeae was tested with 187 N. gonorrhoeae isolates, 81 Neisseria meningitidis isolates, and several strains of other bacterial species. The probe proved to be 100% specific and 100% sensitive. N. gonorrhoeae cells could also be reliably identified in contaminated cultures with the oligonucleotide probe. The 2.6-megadalton cryptic plasmid used as a probe for N. gonorrhoeae was shown to be less sensitive, detecting 179 of 181 N. gonorrhoeae isolates.

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