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A objeção de consciência (OC) no contexto dos cuidados de saúde surge quando um profissional de saúde (PDS) se recusa a participar num certo procedimento, por este não ser compatível com os seus princípios éticos ou morais. A recusa de tratamento no contexto dos cuidados de saúde inclui, para além da OC, outros fatores que podem levar a que um PDS a não querer participar num determinado procedimento. Sendo assim, podemos dizer que a OC é uma forma de recusa de tratamento baseada na consciência. A morte antecipada tem se vindo a tornar uma realidade cada vez mais comum, tratando-se de um procedimento no qual nem todos os profissionais de saúde estão dispostos a participar. Há vários fatores que podem motivar a recusa de tratamento por parte dos PDS neste cenário. Com o objetivo de identificar esses fatores, realizamos uma revisão sistemática, seguindo as guidelines PRISMA. No dia 1 de outubro de 2022, fizemos uma pesquisa na Pubmed, Web of Science e na Scopus. De uma amostra de 693 artigos, 12 foram incluídos na análise final. Identificámos várias motivações que condicionam a recusa ao tratamento, entre as quais motivações legais, técnicas, sociais e a OC. Identificámos ainda 3 motivações principais para a OC, incluindo motivações religiosas, morais/seculares e emocionais/psicológicas. Devemos ter um comportamento compreensivo, e respeitar a posição de cada profissional de saúde, evitando posições de julgamento e discriminatórias. No entanto, devemos garantir que os pacientes tenham acesso aos cuidados. A identificação destas motivações permiterá encontrar soluções que, para além de protegerem a posição dos PDS, possam atenuar potenciais problemas no acesso dos doentes a este tipo de procedimentos. Conscientious objection (CO) in the context of health care arises when a health care professional (HCP) refuses to participate in a certain procedure because it is not compatible with their ethical or moral principles. Refusal to treat in health care includes, in addition to CO, other factors that may lead the HCP not to want to participate in a certain procedure. Therefore, we can say that CO is a form of refusal of treatment based on conscience. Hastened death has become an increasingly reality around the world, being a procedure in which not all HCP are willing to participate. There are several factors that can condition the HCP' refusal to treat in this scenario. With aim of identifying these factors, we performed a systematic review, following de PRISMA guidelines. On 1 October 2022, we searched for relevant articles on Pubmed, Web of Science and Scopus databases. From an initial search of 693 articles, 12 were included in the final analysis. Several motivations that condition refusal to treat were identified, including legal, technical, social, and CO. Three main motivations for CO were also identified, namely religious, moral/secular, and emotional/psychological motivations. We must have an understanding behaviour, respecting the position of each HCP, and avoiding judgmental and discriminatory positions, although, we must ensure that patients have access to care. The identification of these motivations may permit to find solutions that, while protecting HCP' position, may also mitigate the potential problems for patients' access to this type of procedure.

© James Lees, Lucrezia Gorini, Stian Torjussen, Margarida Oliveira, Paula Pinto, Maria Potes Barbas, Madalena Martins, Melanie S. Jones, Victoria Sheppard, Ana Petronilho and Margarida Trindade. Published by Emerald Publishing Limited. This article is published under the Creative Commons Attribution (CC BY 4.0) licence. Anyone may reproduce, distribute, translate and create derivative works of this article (for both commercial & non-commercial purposes), subject to full attribution to the original publication and authors. The full terms of this licence may be seen at http://creativecommons.org/licences/by/4.0/legalcode Purpose: The purpose of this paper is to provide an example of best practice towards enhancing employability in the cross-sectoral labour market for doctorate-holders. This was achieved through an Erasmus+ KA2 (Strategic Partnership) skills development project which created a training programme (TRANSPEER) involving a multi-disciplinary cohort of researchers at a range of career stages, drawn from universities in Norway, Portugal, Sweden and the UK. Design/methodology/approach: Research support staff designed and delivered four transnational training events for the cohort, with the overarching theme of enhancing researcher employability. An initial skills awareness survey of the researcher cohort was undertaken prior to the start of the programme; this survey was repeated after each event. An additional aim of the project was the development of the consortium’s research support staff through exposure to the facilitation techniques and methodologies of their international colleagues. Findings: The findings indicate that transnational collaboration in researcher development enhances the learning environment for participating researchers and provides significant professional development opportunities for both researchers and researcher developers. The findings further suggest the benefits of mixing cohorts across career stages and engaging researchers with novel and interactive approaches on themes not typically addressed in academic competence development offerings. Originality/value: Transversal skills development cooperation between universities – especially transnational cooperation – is rare. Even more so is the professional development of research support staff in a transnational context. This paper outlines the benefits of such collaborative activities.

The demand for products based on natural ingredients is increasing among cosmetic and textile consumers. Cork extracts contain components of interest with special properties, including antioxidant, anti-inflammatory, and antibacterial activities, that might improve the effectiveness of cosmetic formulations currently on the market and may impart new characteristics to textiles. The main goal of this work was to investigate the effect of the incorporation of three cork extracts into two commercial cosmetic formulations (formulation A and B) and evaluate their role as textile dyeing agents. The extracts (E1, E2, and E3) were obtained from cork powder using natural deep eutectic solvents (NADES) (E1-NADES 1: lactic acid:glycerol, E2-NADES 2: lactic acid:glycine, and E3-NADES 3: lactic acid:sodium citrate) and applied in combination with the solvent. The impact of the extracts on the cosmetic formulations’ properties was evaluated in terms of pH, viscosity, antioxidant activity, transdermal permeation capacity, cytotoxicity, and organoleptic characteristics (odor, color, and appearance). The results demonstrated that the cork extracts improved the antioxidant performance of the formulations (90% reduction in DPPH (1,1-difenil-2-picril-hidrazil)). Moreover, low concentrations (5 mg/mL and 10 mg/mL) of extract did not present a cytotoxic effect on keratinocytes. Cotton fabrics were efficiently dyed with the NADES-based cork extracts which conferred to these substrates antioxidant (78% in DPPH reduction) and antibacterial abilities (inhibition halos: 12–15 mm). The application of cork extracts as ingredients in cosmetics or as dyeing/coloration agents for textile coloration is revealed to be a promising and green route to replace harmful ingredients normally used in industry., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. D.S.F. also thanks the Portuguese Foundation for Science and Technology (FCT) for funding (SFRH/BD/147190/2019). A.R. thanks to Portuguese Foundation for Science and Technology (FCT) for is contract under the CEEC-Individual—4th Edition with the reference 2021.02803.CEECIND., info:eu-repo/semantics/publishedVersion

A presente pesquisa busca analisar de que modo uma determinada experiência de formação continuada de professores sobre relações de gênero e sexualidade pode contribuir para novas práticas docentes. Dentro de uma abordagem materialista histórica e dialética e a partir de um roteiro semiestruturado, foram entrevistados nove profissionais que participaram de uma formação continuada sobre a temática em uma escola de educação básica na cidade de Tubarão/SC no ano de 2014. Os resultados indicam a incorporação de práticas pedagógicas críticas em relação aos binarismos de gênero, mas com pouca repercussão em atividades relacionadas à sexualidade.

The shape of wool yarns was changed by laccase-assisted grafting of tyrosine. Prior to tyrosine grafting a cysteine pre-treatment was optimized aiming to increase the amount of thiol reaction groups available. The best operational conditions for laccase-assisted tyrosine grafting were: i) pre-treatment with cysteine (2.2 mM) in a solution of 20 % ethanol, 15 % propylene glycol and 0.5 % benzyl alcohol, pH=10, 40 °C; ii) tyrosine grafting with 3.0 mM tyrosine, 18 U/mL laccase, pH=5, 40 °C. The shape modification was evaluated by number of curly twists determination on the grafted yarn samples. The thermal and mechanical properties of the grafted wool yarns was evaluated by TGA, DSC and breaking strength determination. The amount of free thiols and weight gain were assessed aiming to infer the role of the cysteine pre-treatment on the final tyrosine grafting and shape modification. The laccase-assisted grafting of tyrosine onto wool yarns have influenced the thermal and mechanical properties of the yarns however without compromising their structural integrity for the final application purposes. The developed methodology to impart new shape to wool yarns is presented herein as an environmentally friendly alternative to chemical methods. The new findings revealed great potentialities for application in similar fibers like hair., This study was supported by Chinese Government Scholarship under China Scholar Council [NO. 201906790043] and “the National Nature Science Foundation of China [Grant NO. 52003108]. This study was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation [Norte-01-0145-FEDER-000004] funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Jennifer Noro thanks to FCT for the funding [grand number SFRH/BD/121673]., info:eu-repo/semantics/publishedVersion

O coronavírus (SARS Cov- 2) é um vírus altamente contagioso podendo resultar em casos de síndrome respiratória aguda (SDRA), comprometendo o pulmão de forma importante levando a internação hospitalar na unidade de terapia intensiva. Além do comprometimento pulmonar o SARS Cov-2 pode resultar em complicações cardiovasculares, neurológicas, psicológicas e musculoesqueléticas, contribuindo consequentemente no tempo de internação hospitalar. Dada a essas condições clínicas a mobilização precoceé uma prática segura e viável que pode ser utilizada por meio do fisioterapeuta para reestabelecer a força muscular e a recuperação funcional dos pacientes internados na unidade de terapia intensiva, e consequentemente melhorar a qualidade de vida, reduzir os efeitos nocivos da doença, particularmente na função muscular e cardiorrespiratória, na mobilidade e funcionalidade. O Objetivo desse estudo foi descrever a importância da realização da mobilização precoce em pacientes com Covid-19 internados na Unidade de Terapia Intensiva.Trata-sede uma revisãobibliográficarealizada por meio de pesquisas nas seguintes bases de dadosPubMed, LILACS eSciELOcom os descritores“COVID-19”, “mobilização precoce”, “unidade de terapia intensiva”, “internação hospitalar” e “atuação fisioterapêutica na UTI”, emportuguês e inglês, nos últimos seteanos. Os artigos foram avaliados por três pesquisadoras por meio da leitura dos resumos e dos critérios de inclusão e exclusão.Estudos tem demostrado que pacientes com COVID-19 internados na unidade de terapia intensiva desenvolvem grandes perdas funcionais tanto pela patologia da doença, como pelos dias de internação hospitalar na UTI, desta forma a fisioterapia por meio da mobilização precoce pode contribuir por meio de suas competências na manutenção da capacidade funcional, na prevenção e redução dos efeitos deletérios da imobilismo, nas disfunções neuromusculares, reduzindo o tempo da ventilação mecânica invasiva e da internação hospitalar desses pacientes, além de diminuir consideravelmente as taxas de reinternação hospitalar.

A transmissão intergeracional da parentalidade refere-se a um padrão de continuidade das práticas parentais entre as gerações. A literatura tem descrito o papel das práticas parentais na infância na saúde mental na idade adulta, contudo, o papel mediador da saúde mental nesta relação ainda não foi explorado de forma sistemática. Assim, neste estudo, pretendemos testar o papel mediador da ansiedade e depressão na relação entre a parentalidade na infância e as práticas atuais. Neste estudo participaram um total de 300 pais e mães, com idades compreendidas entre os 26 e os 61 anos (M=41; DP=5.5), e maioritariamente do sexo feminino (86%). Os participantes preencheram um conjunto de três questionários de autorrelato: EMBU-P, o EMBU-MI e o BSI. Os resultados revelaram efeitos totais significativos e positivos na relação entre a rejeição na infância e adolescência (da mãe e do pai) e práticas atuais de suporte emocional, e na relação entre memórias de sobreproteção pelo pai e práticas atuais de rejeição e de tentativa de controlo. Além disso, foi encontrado um efeito de mediação parcial da depressão na relação entre suporte emocional (da mãe e do pai) e práticas atuais de suporte emocional e um efeito indireto significativo da depressão na relação entre as experiências de suporte emocional pelo pai e pela mãe e as práticas parentais atuais de rejeição e tentativa de controlo. Estes resultados sugerem que é essencial promover práticas parentais de suporte emocional enquanto fator protetor da sintomatologia na idade adulta e promotor da transmissão intergeracional destas práticas. The intergenerational transmission of parenting refers to a pattern of continuity of parenting practices between generations. The literature has described the role of parenting later in adults’ mental health, however, the mediating role of mental health in this relationship has not yet been systematically explored. Thus, in this study, we intend to test the mediating role of anxiety and depression in the relationship between childhood parenting and current parental practices. A total of 300 fathers and mothers participated in this study, aged between 26 and 61 years (M=41; SD=5.5), mostly female (86%). Participants completed a set of three self-report questionnaires: EMBU-P, EMBU-MI, and BSI. The results revealed significant and positive overall effects on the relationship between rejection (from both mother and father) and current emotional support practices, and on the relationship between overprotective memories with the father and current rejection and control-attempt practices. In addition, a partial mediating effect of depression was found on the relationship between emotional support (from the mother and father) and current emotional support practices and a significant indirect effect of depression on the relationship between father and mother's emotional support experiences and current parental practices of rejection and attempt to control. These results suggest that it is essential to promote parenting practices of emotional support as a protective factor for symptoms in adulthood and the intergenerational transmission of these practices.

Skin cancer happens when skin cells grow and multiply in an uncontrolled and disordered way. Normally, new skin cells form when the cells age and die or when they are damaged. When this process does not work as it should, cells (some of which may be abnormal cells) grow rapidly. Aiming to review in the literature the dangers of ultraviolet rays for skin cancer. This is an integrative literature review carried out through the SciELO, LILACS, BDENF and MEDLINE databases, through the following DeCS: “Neoplasms”, “Skin Neoplasms” and “Ultraviolet Rays”. As inclusion criteria: articles available online, in full, in Portuguese, English and Spanish, that addressed the topic in the last ten years. As exclusion criteria: articles that did not cover the topic and repeated studies between databases. The main cause of skin cancer is excessive exposure to sunlight, especially when it results in sunburn and blisters. The sun's ultraviolet (UV) rays damage the skin's DNA, causing abnormal cells to form. These abnormal cells divide rapidly in a disorganized way, forming a mass of cancer cells. The skin is the largest organ in your body, so it needs as much attention as any other health problem. What may seem like an innocent cosmetic imperfection may not be. Performing regular skin checks is important for everyone and is especially important if the person already has a family history of a person at increased risk of skin cancer. El cáncer de piel ocurre cuando las células de la piel crecen y se multiplican de forma descontrolada y desordenada. Normalmente, las nuevas células de la piel se forman cuando las células envejecen y mueren o cuando se dañan. Cuando este proceso no funciona como debería, las células (algunas de las cuales pueden ser células anormales) crecen rápidamente. Con el objetivo de revisar en la literatura los peligros de los rayos ultravioleta para el cáncer de piel. Se trata de una revisión integrativa de la literatura realizada a través de las bases de datos SciELO, LILACS, BDENF y MEDLINE, a través de los siguientes DeCS: “Neoplasias”, “Neoplasias de la Piel” y “Rayos Ultravioleta”. Como criterios de inclusión: artículos disponibles en línea, completos, en portugués, inglés y español, que abordaran el tema en los últimos diez años Como criterios de exclusión: artículos que no abordaran el tema y estudios repetidos entre bases de datos. La principal causa del cáncer de piel es la exposición excesiva a la luz solar, especialmente cuando provoca quemaduras solares y ampollas. Los rayos ultravioleta (UV) del sol dañan el ADN de la piel y provocan la formación de células anormales. Estas células anormales se dividen rápidamente de manera desorganizada, formando una masa de células cancerosas. La piel es el órgano más grande de tu cuerpo, por lo que necesita tanta atención como cualquier otro problema de salud. Lo que puede parecer una imperfección cosmética inocente puede no serlo. Realizar controles regulares de la piel es importante para todos y es especialmente importante si la persona ya tiene antecedentes familiares de una persona con mayor riesgo de cáncer de piel. O câncer de pele acontece quando as células da pele crescem e se multiplicam de forma descontrolada e desordenada. Normalmente, novas células da pele se formam quando as células envelhecem e morrem ou quando são danificadas. Quando esse processo não funciona como deveria, ocorre um rápido crescimento de células (algumas das quais podem ser células anormais). Tendo como objetivo revisar na literatura os perigos dos raios ultravioleta para o câncer de pele. Trata-se de uma revisão integrativa de literatura realizada através das bases de dados SciELO, LILACS, BDENF e MEDLINE, através dos seguintes DeCS: “Neoplasias”, “Neoplasias Cutâneas” e “Raios Ultravioleta”. Como critérios de inclusão: artigos disponíveis online, na íntegra, nos idiomas português, inglês e espanhol, que abordassem a temática nos últimos dez anos.omo critérios de exclusão: artigos que não contemplavam o tema e estudos repetidos entre bases de dados. A principal causa do câncer de pele é a exposição excessiva à luz solar, especialmente quando resulta em queimaduras solares e bolhas. Os raios ultravioleta (UV) do sol danificam o DNA da pele, causando a formação de células anormais. Essas células anormais se dividem rapidamente de maneira desorganizada, formando uma massa de células cancerígenas. A pele é o maior órgão do seu corpo, sendo assim precisa de tanta atenção quanto qualquer outro problema de saúde. O que pode parecer uma imperfeição cosmética inocente, pode não ser. Realizar verificações regulares da pele é importante para todos e é especialmente importante se a pessoa já tem histórico na família por uma pessoa com risco aumentado de câncer de pele.

O presente artigo, apresenta resultados de uma pesquisa que teve como problemática investigar a configuração curricular do Curso de Pedagogia da Unisul referente a incorporação de conhecimentos para a docência na Educação Infantil, tendo em conta a implementação das Diretrizes Curriculares Nacionais para o Curso de Graduação em Pedagogia de 2006. Para a realização da pesquisa utilizou-se como procedimentos metodológicos a análise documental do currículo do Curso de Pedagogia da Unisul, bem como, dados de uma entrevista com a coordenação. Para a organização e análises dos dados optou-se pelo Técnica de Análise de Conteúdo. Os dados revelaram a presença de unidades de aprendizagem específicas relativas à infância e às crianças de forma a indicar uma articulação teórico-prática para a docência na Educação Infantil e anos iniciais do Ensino Fundamental, bem como, a existência de uma disparidade entre carga horária destinada à formação para a docência na Educação Infantil e Ensino Fundamental.

Eutectic-based formulations were developed aiming to modify the shape and improve the mechanical properties of Asian hair. These formulations comprised a eutectic mixture (choline chloride and urea) as a solvent, a natural reducing agent (l-cysteine), and a decapeptide. The treatment of Asian hair with the eutectic-based formulations increased the resistance of hair to mechanical wear by about 10\\% when compared with hair treated with a styling commercial formulation (chemical treatment). For these samples, the change in hair shape is associated with the presence of cysteine and the decapeptide, which participates in the disruption and in the rearrangement of new disulfide bonds with the keratin fibers. The eutectic-based formulations here presented show great potential to be used as a new class of hair styling agents in haircare research. These new formulations will avoid/reduce the application of harsh chemicals, which induce irreversible damage in hair structure and have a negative impact on the environment., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of the UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020., info:eu-repo/semantics/publishedVersion

O estágio foi realizado na Herdade da Courela do Paço, em Elvas, onde a empresa AgroElvas cultivou 4,5 ha de milho para grão, durante o ano de 2020. O estágio teve como objetivo acompanhar o itinerário técnico da cultura e posteriormente efetuar a determinação dos custos de produção (unitário e total). O itinerário técnico iniciou-se a 16 de maio de 2020 com a preparação do solo, tendo a sementeira em linhas ocorrido a 18 de maio. Semeou-se a variedade de ciclo médio (FAO 500) designada P0937, da firma Pionner. A densidade de sementeira foi de 91.000 sementes/ha, do que se estima ter resultado uma população de 85.000 plantas/ha, suficiente para uma boa produção. A fertilização seguiu um esquema fixo definido pelo agricultor. A rega, por gravidade, consumiu 6.200 m3/ha. Os tratamentos fitossanitários foram todos preventivos, maioritariamente a doses reduzidas. Durante os meses de junho a outubro fez-se um acompanhamento semanal da cultura, no qual se registou a evolução dos estados fenológicos, tendose observado que a cultura se desenvolveu sem problemas. A colheita, realizada a 17 de outubro de 2020, permitiu obter uma produção unitária de 12.450 kg/ha. Como o agricultor armazena, em instalações próprias, e vende o milho diretamente ao consumidor final, mais tarde que os restantes produtores, consegue obter um preço superior ao de mercado, atingindo 230 €/ton. Apesar de alguma tecnologia de produção ser passível de melhoria, nomeadamente a fertilização, a rega e o controlo fitossanitário, o agricultor consegue obter uma margem bruta (1.193 €/ha) muito superior ao verificado noutros estudos. No final do trabalho são efetuadas algumas propostas de melhoria e feita a recomendação de efetuar anualmente o cálculo da conta de cultura (custos e receitas) para aferir da rentabilidade da cultura de milho-grão nestas condições de produção. The internship was held at Herdade da Courela do Paço, in Elvas, where the company AgroElvas cultivated 4.5 ha of corn for grain, during 2020. The internship aimed to monitor the technical itinerary of the crop and subsequently make the determination production costs (unit and total). The technical itinerary began on May 16, 2020 with soil preparation, with sowing in rows taking place on May 18. The medium cycle variety (FAO 500) designated P0937, from Pioneer, was sown. The sowing density was 91,000 seeds/ha, which is estimated to have resulted in a population of 85,000 plants/ha, sufficient for good production. Fertilization followed a fixed schedule defined by the farmer. Irrigation, by gravity, consumed 6,200 m3/ha. Phytosanitary treatments were all preventive, mostly at reduced doses. During the months of June to October, a weekly monitoring of the crop was carried out, in which the evolution of the phenological states was registered, and it was observed that the crop developed without any problems. The harvest, carried out on October 17, 2020, allowed to obtain a unit production of 12,450 kg/ha. As the farmer stores, in his own facilities, and sells corn directly to the final consumer, later than the other producers, he manages to obtain a price higher than the market price, reaching €230/ton. Although some production technology can be improved, namely fertilization, irrigation and phytosanitary control, the farmer manages to obtain a gross margin (1,193 €/ha) much higher than that verified in other studies. At the end of the work, some improvement proposals are made and a recommendation is made to annually calculate the crop account (costs and revenues) to assess the profitability of the corn-grain crop under these production conditions.

The methylimidazolium chloride ionic liquid (IL) was successfully used for the dissolution of cotton cellulose. IL and IL-cellulose were investigated by X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy analyses. The obtained IL-cellulose was applied to wool and hair keratin fiber surfaces, forming a fine coating layer. From FT-IR and XRD analyses, the results suggested the reduction of hydrogen bonds and crystallinity in IL-cellulose-coated wool fibers. This effect was caused by covering the wool with the IL-cellulose coating, and good intrinsic mechanical properties were observed. Moreover, the dimensional performance of coated keratin fibers presented length variations of around 8 and 12\% under wet and dry conditions for wool and hair fibers, respectively. Coated keratin fibers could be of great value for a cleaner process in the cosmetic area associated with their ability to stretch and curl when triggered by humidity., Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020, info:eu-repo/semantics/publishedVersion

Cosmetics procedures and products combined with environmental insults and daily routines induce irreversible changes in hair. As result of damage, the hair loses some of its properties like strength, elasticity, and smoothness. Recent studies revealed the positive effects of protein-based cosmetics in providing protection to hair. Additionally, these cosmetic products have also shown a great ability to modify hair fibers. We review the effect of protein-based cosmetic formulations on hair properties like color, scent, strength, shape, and volume, highlighting the potential of keratin-based particles and keratin-fusion proteins. In the future, incorporating multifunctional proteins and peptides in the development of alternative hair formulations will result in advanced, sustainable, ecofriendly cosmetic products with a great impact on the cosmetic industry., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of the UIDB/04469/2020 unit. A.T. and M.M. have work contracts supported by the European Social Fund under the scope of Norte2020 – Programa Operacional Regional do Norte (NORTE-06-3559-FSE-000141)., info:eu-repo/semantics/publishedVersion

O câncer de pele acontece quando as células da pele crescem e se multiplicam de forma descontrolada e desordenada. Normalmente, novas células da pele se formam quando as células envelhecem e morrem ou quando são danificadas. Quando esse processo não funciona como deveria, ocorre um rápido crescimento de células (algumas das quais podem ser células anormais). Tendo como objetivo revisar na literatura os perigos dos raios ultravioleta para o câncer de pele. Trata-se de uma revisão integrativa de literatura realizada através das bases de dados SciELO, LILACS, BDENF e MEDLINE, através dos seguintes DeCS: “Neoplasias”, “Neoplasias Cutâneas” e “Raios Ultravioleta”. Como critérios de inclusão: artigos disponíveis online, na íntegra, nos idiomas português, inglês e espanhol, que abordassem a temática nos últimos dez anos.omo critérios de exclusão: artigos que não contemplavam o tema e estudos repetidos entre bases de dados. A principal causa do câncer de pele é a exposição excessiva à luz solar, especialmente quando resulta em queimaduras solares e bolhas. Os raios ultravioleta (UV) do sol danificam o DNA da pele, causando a formação de células anormais. Essas células anormais se dividem rapidamente de maneira desorganizada, formando uma massa de células cancerígenas. A pele é o maior órgão do seu corpo, sendo assim precisa de tanta atenção quanto qualquer outro problema de saúde. O que pode parecer uma imperfeição cosmética inocente, pode não ser. Realizar verificações regulares da pele é importante para todos e é especialmente importante se a pessoa já tem histórico na família por uma pessoa com risco aumentado de câncer de pele.

Discussion about the information science as a knowledge field, its concept, scope and evolution, paying attention to its interdisciplinary side, especially in relation to the cognitive sciences. The main cognitive approaches, both traditional and contemporary, as well as their respective origins and influences on information science are dealt with. Studies developed by researchers of the Cognitive Studies in Information Science Group (GECCI) from the Federal University of Minas Gerais, Brazil, are presented.

The perming of hair is a common styling procedure with negative impact on the overall properties of the hair fibers. Usually, this process uses harsh chemicals to promote the disruption of disulfide bonds and the formation of new bonds to change the shape of hair. Here, we explored bovine serum albumin (BSA), silk fibroin (SF), keratin and two fusion recombinant proteins (KP-UM and KP-Cryst) as new perming agents. A phosphate buffer prepared at different pH values (5, 7 and 9) was used to apply the proteins to virgin Asian hair, and a hot BaByliss was used to curl the hair fibers. To assess the potential of the protein formulations for hair styling, the perming efficiency and the perming resistance to wash were measured. Furthermore, the fiber water content was evaluated to assess if the proteins protected the hair during the styling process. Despite all of the proteins being able to assist in the curling of Asian hair, the best perming efficiency and perming resistance to wash results were observed for BSA and keratin. These proteins showed perming efficiency values close to that measured for a commercial perming product (chemical method), particularly at pH 5 and 9. The increase in the hairs internal and external water contents revealed a protective effect provided by the proteins during the application of heat in the styling procedure. This study shows the potential of proteins to be used in the development of new eco-friendly hair styling products., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit., info:eu-repo/semantics/publishedVersion

Projeto "Characterization of the T. gondii TBCB interactome" (UID/CVT/00276/2019) Mestrado em Tecnologias Moleculares em Saúde Submitted by Maria da Luz Antunes (mluz.antunes@estesl.ipl.pt) on 2021-12-23T19:48:19Z No. of bitstreams: 1 Production of T. gondii transgenic strains to characterize the tubulin cofactor B interactome.pdf: 1875000 bytes, checksum: dfde6d25e038639dee586cf1826e3914 (MD5) Made available in DSpace on 2021-12-23T19:48:19Z (GMT). No. of bitstreams: 1 Production of T. gondii transgenic strains to characterize the tubulin cofactor B interactome.pdf: 1875000 bytes, checksum: dfde6d25e038639dee586cf1826e3914 (MD5) Previous issue date: 2021 N/A

The follicular route is an important drug penetration pathway in any topical application, either concerning dermatological and cosmetic skin treatments or any transdermal administration regimen. Efficient transport into follicles will depend on drug inherent properties but also on the chosen vehicle. The main study goal was to compare several systems for the delivery to the hair bulb of two fluorescent molecules of different water affinities: the hydrophobic Nile Red and the quite similar but hydrophilic Nile Blue. Three common nanoparticle types were compared in terms of encapsulation efficiency and stability: liposomes, ethosomes and polymeric nanoparticles. A liquid serum-like formulation was also developed, adjusting the final ethanol amount to the type of dye to be solubilized. Then, this formulation and the nanoparticle systems that successfully passed characterization and stability stages were further studied on their ability to reach the bulb. The serum formulation was able to deliver, both drug models, to deeper follicular regions than nanoparticles. Attending to the envisioned zone target of the follicle, the simplest approach proved to be the best choice from all the systems tested in this work. Nonetheless, nanocarriers and the inherent complexity of their manufacturing processes may be justified under very specific requirements., The author Cristiana Costa would like to acknowledge his PhD scholarship funded by Portuguese Foundation for Science and Technology (FCT) (SFRH/BD/139522/2018). The author Bruno Fernandes would like to acknowledge his PhD scholarship funded by FCT (SFRH/BD/131824/2017). The author Diana Guimarães would like to acknowledge his PhD scholarship funded by FCT (SFRH/BD/140321/2018). This study was supported by the FCT under the scope of BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte., info:eu-repo/semantics/publishedVersion

Objective The capacity of hair to absorb water causes changes in its physical and cosmetic properties under different environmental conditions. Hence, the control of hair volume in variable relative humidity settings is an important topic in cosmetics. The behaviour of two types of hair, Caucasian and Asian, was studied regarding their volume change in different relative humidity conditions. The ability of a peptide as a hair volume treatment was evaluated in two climate control formulations. Methods Tresses of the two types of hair were tested in two relative humidity (RH) conditions: (A) variable relative humidity (2 hours 40% RH, followed by 2 hours 90% RH and 2 hours of 40% RH), and (B) continuous high relative humidity (90% RH for 6 hours). Changes in the hair tress volume were assessed throughout time. Hair treated with two climate control formulations, with and without a peptide (KP peptide), were tested under the two relative humidity conditions. Results Caucasian hair had a higher change in volume compared to the Asian hair in variable and high relative humidity conditions. The hair volume increase when subject to high air humidity, and it was lower with the incorporation of a peptide into climate control formulations. Conclusion Caucasian hair showed higher volume than Asian hair when submitted to both relative humidity conditions. The incorporation of the peptide into the climate control formulations, a base (mostly composed of water 94%) and an ethanolic, was found to reduce the volume of Caucasian hair tresses. The presence of the peptide improved the hair volume change more than 60% in high relative humidity conditions., We thank the Industry and Processes Laboratory (LIP) from Centre of Biological Engineering (CEB) for allowing the use of the climate chamber Binder KBF 115. This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI01-0145-FEDER-006684) and under the Project RECI/BBB-EBI/ 0179/2012 (FCOMP-01-0124-FEDER-027462). This study was also supported by BioTecNorte operation (NORTE-01-0145-FEDER000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. C elia F. Cruz and Artur Ribeiro thank FCT for SFRH/BD/ 100927/2014 and SFRH/BPD/98388/2013 grants, respectively., info:eu-repo/semantics/publishedVersion

Dispersions in transcutol/isopropyl myristate make C60 fullerene molecules suitable for transdermal delivery. We found that C60 can successfully permeate the skin using pig skin in Franz diffusion cells. Molecular dynamics simulations and transmission electron microscopy confirmed these observations. Basic cosmetic formulations with transcutol/ isopropyl myristate without harsh organic solvents show a high potential for delivery of C60 for biopharmaceutical and cosmetics applications., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI01-0145-FEDER-006684).). Artur Ribeiro thanks FCT for the SFRH\BPD\98388\2013 grant., info:eu-repo/semantics/publishedVersion

Chemical straightening of curly human hair fibres involves the use of strong reducing agents at alkaline pH. Human hair is made of keratin, and the fixation of fibre shape involves the reduction and reformation of new disulphide bonds between keratin molecules. Here, we propose an alternative and green methodology using keratin peptide sequences (10-13 residues) derived from the human genome. In a previous study, we analysed 1235 cysteine-containing peptides encoded by all human genes of hair keratin and keratin-associated proteins. These peptide fragments have been designed by nature to interact with keratin. Here we tested eight peptides, which were select based on their affinity for human hair keratin solution as shown by Matrix-Assisted Laser-Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF/TOF) and by molecular dynamics simulation. The peptides were characterized in detail regarding their ability to act as hair straightening modulators and to improve the tensile strength and elasticity of hair. Of the eight tested peptides, PepE, PepG and KP showed the highest ability to interact with a keratin peptide model, and to improve hair mechanical properties and straightening efficiency. The proposed solutions presented here will replace harsh reducing agents at alkaline pH by peptide formulations acting at neutral pH to change hair shape through the re-conformation of disulphide bonds. Here, we provide experimental evidence which explains at a molecular level how keratin decapeptides can interact with large keratin molecules in human hair, opening an innovative green approach to changing the shape of hair fibre., This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of the UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and under the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). This study was also supported by BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Célia F. Cruz and Artur Ribeiro thank FCT for SFRH/BD/100927/2014 and SFRH/BPD/98388/2013 grants, respectively., info:eu-repo/semantics/publishedVersion

The present research relates to a fusion protein comprising a chromogenic blue ultramarine protein (UM) bound to a keratin-based peptide (KP). The KP-UM fusion protein explores UM chromogenic nature together with KP affinity towards hair. For the first time a fusion protein with a chromogenic nature is explored as a hair coloring agent. The KP-UM protein colored overbleached hair, being the color dependent on the formulation polarity. The protein was able to bind to the hair cuticle and even to penetrate throughout the hair fibre. Molecular dynamics studies demonstrated that the interaction between the KP-UM protein and the hair was mediated by the KP sequence. All the formulations recovered the mechanical properties of overbleached hair and KP-UM proved to be safe when tested in human keratinocytes. Although based on a chromogenic non-fluorescent protein, the KP-UM protein presented a photoswitch phenomenon, changing from chromogenic to fluorescent depending on the wavelength selected for excitation. KP-UM protein shows the potential to be incorporated in new eco-friendly cosmetic formulations for hair coloration, decreasing the use of traditional dyes and reducing its environmental impact., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. We also acknowledge the strategic program UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) funded by national funds through Fundação para a Ciência e a Tecnologia (FCT) and by the ERDF through the COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI). Ana Tinoco, Filipa Gonçalves and Carla Silva thanks FCT for funding the scholarships with the references SFRH/BD/114035/2015, FRH/BD/114684/2016 and IF/00186/2015, respectively., info:eu-repo/semantics/publishedVersion

In Information Science context, Subject Analysis is considered one of the most important stages in Indexing and the indexer is the responsible for this activity. An interdisciplinarity with Linguistics, Cognitive Science and Logics is observed during the development of this activity by the indexer. The study discusses factors of these areas that interfere in Subject Analysis. Linguistic factors act in concert with logical and cognitive factors in the intellectual activities that comprise Subject Analysis. So close is their interaction that it is not possible to determine where the influence of one begins or finishes. This reference base is required in order to understand what the text in question is about and in order to assimilate new knowledge. When analyzing a text, the indexer searches the reference base for stored information about the theme of the text and if found comprehension occurs; however, if not found, comprehension is thwarted

O Relatório Final de Mestrado, denominado “O Tangram, veículo de integração de aprendizagens com a Matemática: abordagem no 1.º ano do Ensino Básico”, pretende descrever e refletir de forma sucinta sobre o Estágio em 1.º Ciclo do Ensino Básico (CEB), no âmbito do Mestrado em Educação Pré-Escolar e Ensino do 1.º Ciclo do Ensino Básico. O Relatório Final também apresenta uma proposta de investigação de natureza qualitativa com cunho descritivo e interpretativo, cujas questões de pesquiza são: Que oportunidades de aprendizagem o “Cenário Tangram” proporciona a crianças do 1.º ano? e Qual a reflexão da investigadora sobre a sua orquestração das atividades matemáticas das crianças, em tal contexto? Os resultados deste estudo apontam que o “Cenário Tangram”, é um ambiente integrador de diferentes domínios: Matemática, Tecnologia da Informação e Comunicação, Educação Financeira, Estudo do Meio, Português, Expressão e Educação Plástica, num contexto lúdico onde se pretendeu oferecer oportunidades aos alunos de estabelecerem uma relação positiva e ativa com aquisição de conhecimento. O papel da Investigadora foi fundamentalmente mediadora da aprendizagem dos alunos, onde colocou questões de sugerir ação e de resolver problemas. Para finalizar este Relatório Final são feitas algumas Considerações Finais sobre as aprendizagens desenvolvidas pela Investigadora durante o estudo, as suas vivências ao longo do Estágio em 1.º CEB e na elaboração deste Relatório Final.

Conventional chemical treatments imply the use of harsh chemicals which can cause serious adverse effects on consumers health, hair fibre and environment. In this work was reported for the first time the modelling shape of Asian hair by oligopeptide coating synthesized by a protease. ?-Chymotrypsin from Bovine pancreas catalysed the oligomerization of the tri-peptides, KCL (Lysine-Cysteine-Leucine) ethyl ester and KSL (Lysine-Serine-Leucine) ethyl ester in aqueous media. The synthesis reaction gave rise to oligopeptides with DP?=?6, which were characterized by mass spectra (MALDI-TOF). The in situ synthesis reaction onto the hair surface, under controlled pH (8.59) and temperatures between 25?°C and 55?°C, formed a coating layer inducing changes in the hair shape from straight to curly. Qualitative and quantitative characterization of coated hair was performed by image visualization and by perming efficiency assessment. The developed strategy revealed to be a safer and cleaner alternative to the conventional chemical treatments generally used for hair shape modification. Low cost associated to the catalyst and lower side effects to the hair fibre, health consumer and environment might lead to the development of a cost-effective and sustainable approach in the hair care industry., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Carla Silva acknowledges Portuguese Foundation for Science and Technology (FCT) for the funding (SFRH/IF/00186/2015)., info:eu-repo/semantics/publishedVersion

The Textile Institute Book Series, This chapter summarizes the essential parameters for enzyme stabilization during industrial applications providing an overview of enzyme's chemistry basics related with their structure and function. The strategies for enzyme immobilization and incorporation into diverse formulations imply the maintenance of their catalytic efficiency. Thus, we outlined in this chapter the more recent studies toward the enzyme stability/activity underlying these approaches. Different applications of stabilized enzymes have been updated with special focus on the textile field., (undefined), info:eu-repo/semantics/publishedVersion

Hair mechanical properties are primary indicators of hair integrity. Here we describe a methodology based on hair's resistance to mechanical wear to evaluate cosmetic treatments. Using a vibrational vortex cycle, we developed a protocol that relies on the plastic deformation and degradation of hair, due to frictional forces between hardened steel balls and hair fragments. We demonstrate that the balls when in contact with the hair fragments promoted extended hair degradation. The degree of degradation was related to hair's resistance to mechanical wear. Lower degradation rates reflect high resistance to mechanical wear. We found differences according to hair ethnicity and type of treatment. This methodology shows great potential for the screening and evaluation of the effect of cosmetic treatments on hair., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER000004) funded by the European Regional Development Fund under the scope of Norte 2020 - Programa Operacional Regional do Norte. AT thanks FTC for funding the scholarship with the reference SFRH/BD/114035/2015., info:eu-repo/semantics/publishedVersion

Plants are subjected to adverse conditions being outer protective tissues fundamental to their survival. Tree stems are enveloped by a periderm made of cork cells, resulting from the activity of the meristem phellogen. DNA methylation and histone modifications have important roles in the regulation of plant cell differentiation. However, studies on its involvement in cork differentiation are scarce despite periderm importance. Cork oak periderm development was used as a model to study the formation and differentiation of secondary protective tissues, and their behavior after traumatic wounding (traumatic periderm). Nuclei structural changes, dynamics of DNA methylation, and posttranslational histone modifications were assessed in young and traumatic periderms, after cork harvesting. Lenticular phellogen producing atypical non-suberized cells that disaggregate and form pores was also studied, due to high impact for cork industrial uses. Immunolocalization of active and repressive marks, transcription analysis of the corresponding genes, and correlations between gene expression and cork porosity were investigated. During young periderm development, a reduction in nuclei area along with high levels of DNA methylation occurred throughout epidermis disruption. As cork cells became more differentiated, whole nuclei progressive chromatin condensation with accumulation in the nuclear periphery and increasing DNA methylation was observed. Lenticular cells nuclei were highly fragmented with faint 5-mC labeling. Phellogen nuclei were less methylated than in cork cells, and in lenticular phellogen were even lower. No significant differences were detected in H3K4me3 and H3K18ac signals between cork cells layers, although an increase in H3K4me3 signals was found from the phellogen to cork cells. Distinct gene expression patterns in young and traumatic periderms suggest that cork differentiation might be under specific silencing regulatory pathways. Significant correlations were found between QsMET1, QsMET2, and QsSUVH4 gene expression and cork porosity. This work evidences that DNA methylation and histone modifications play a role in cork differentiation and epidermis induced tension-stress. It also provides the first insights into chromatin dynamics during cork and lenticular cells differentiation pointing to a distinct type of remodeling associated with cell death.