12 results on '"Mackova H"'
Search Results
2. Encapsulation of human serum albumin in submicrometer magnetic poly(lactide-co-glycolide) particles as a model system for targeted drug delivery
- Author
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Shubhra Quazi T.H., Macková Hana, Horák Daniel, Fodor-Kardos Andrea, Tóth Judit, Gyenis János, and Feczkó Tivadar
- Subjects
Polymers and polymer manufacture ,TP1080-1185 - Abstract
Two types of iron oxide nanoparticles were synthesized by coprecipitation of Fe(II) and Fe(III) chlorides: water-dispersible γ-Fe2O3 and organic solvent-dispersible oleic acid-coated Fe3O4 particles. The nanoparticles, together with human serum albumin (HSA) serving as a model for a protein-type drug, were then incorporated in poly(lactide-co-glycolide) (PLGA) particles using double emulsion solvent evaporation technique. Morphology, size and particle size distribution of the resulting particles was analyzed by electron microscopy and dynamic light scattering. Iron oxide and HSA encapsulating efficiency was determined by Prussian Blue staining and micro-BCA assay, respectively.
- Published
- 2013
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3. The Nature of Chemical Bonding in Lewis Adducts as Reflected by 27 Al NMR Quadrupolar Coupling Constant: Combined Solid-State NMR and Quantum Chemical Approach.
- Author
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Kobera L, Czernek J, Abbrent S, Mackova H, Pavlovec L, Rohlicek J, and Brus J
- Abstract
Lewis acids and Lewis adducts are widely used in the chemical industry because of their high catalytic activity. Their precise geometrical description and understanding of their electronic structure are a crucial step for targeted synthesis and specific use. Herein, we present an experimental/computational strategy based on a solid-state NMR crystallographic approach allowing for detailed structural characterization of a wide range of organoaluminum compounds considerably differing in their chemical constitution. In particular, we focus on the precise measurement and subsequent quantum-chemical analysis of many different
27 Al NMR resonances in the extremely broad range of quadrupolar coupling constants from 1 to 50 MHz. In this regard, we have optimized an experimental strategy combining a range of static as well as magic angle spinning experiments allowing reliable detection of the entire set of aluminum sites present in trimesitylaluminum (AlMes3 ) reaction products. In this way, we have spectroscopically resolved six different products in the resulting polycrystalline mixture. All27 Al NMR resonances are precisely recorded and comprehensively analyzed by a quantum-chemical approach. Interestingly, in some cases the recorded27 Al solid-state NMR spectra show unexpected quadrupolar coupling constant values reaching up to ca. 30 MHz, which are attributed to tetra-coordinated aluminum species (Lewis adducts with trigonal pyramidal geometry). The cause of this unusual behavior is explored by analyzing the natural bond orbitals and complexation energies. The linear correlation between the quadrupolar coupling constant value and the nature of bonds in the Lewis adducts is revealed. Moreover, the27 Al NMR data are shown to be sensitive to the geometry of the tetra-coordinated organoaluminum species. Our findings thus provide a viable approach for the direct identification of Lewis acids and Lewis adducts, not only in the investigated multicomponent organoaluminum compounds but also in inorganic zeolites featuring catalytically active trigonal (AlIII ) and strongly perturbed AlIV sites.- Published
- 2018
- Full Text
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4. Biopolymer strategy for the treatment of Wilson's disease.
- Author
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Vetrik M, Mattova J, Mackova H, Kucka J, Pouckova P, Kukackova O, Brus J, Eigner-Henke S, Sedlacek O, Sefc L, Stepanek P, and Hruby M
- Subjects
- Animals, Cellulose pharmacokinetics, Chitosan pharmacokinetics, Copper, Copper Radioisotopes administration & dosage, Copper Radioisotopes pharmacokinetics, Gastrointestinal Tract metabolism, Hepatolenticular Degeneration metabolism, Oxyquinoline pharmacokinetics, Rats, Wistar, Cellulose administration & dosage, Chitosan administration & dosage, Hepatolenticular Degeneration drug therapy, Oxyquinoline administration & dosage
- Abstract
Wilson's disease is a genetic disorder that causes excessive accumulation of copper in the body, leading to toxic damage, especially in the liver and nervous system. The current treatment cause burdensome side effects. We describe the use of chemically modified biopolymer carriers based on microcrystalline cellulose and chitosan containing the highly specific copper chelator 8-hydroxyquinoline as a new type of therapy for Wilson's disease. The chelators can scavenges copper ions released from food during digestion and copper ions present in secretions in the gastrointestinal tract. Because the chelator is covalently bound to indigestible biopolymer carriers (crosslinked chitosan or modified cellulose), it is not taken up by the gastrointestinal tract and it can be eliminated through the feces, avoiding unwanted side effects. This concept was tested on Wistar rats, which received a radioactive
64 CuCl2 solution together with the polymers with covalently bound 8-hydroxyquinoline through a gastric probe.64 Copper complex uptake from the gastrointestinal tract was significantly inhibited by both chelating polymers. With the modified polymers, the presence of64 Cu was detected mostly in the gastrointestinal tract, not in the internal organs. These findings indicate modified cellulose and crosslinked chitosan, with covalently bound 8-hydroxyquinoline exhibited the potential to be excellent therapeutics for treating Wilson's disease., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2018
- Full Text
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5. Co-encapsulation of human serum albumin and superparamagnetic iron oxide in PLGA nanoparticles: part II. Effect of process variables on protein model drug encapsulation efficiency.
- Author
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Shubhra QT, Feczkó T, Kardos AF, Tóth J, Mackova H, Horak D, Dósa G, and Gyenis J
- Subjects
- Humans, Immobilized Proteins chemistry, Nanoparticles ultrastructure, Particle Size, Polylactic Acid-Polyglycolic Acid Copolymer, Drug Delivery Systems, Ferric Compounds chemistry, Lactic Acid chemistry, Models, Chemical, Nanoparticles chemistry, Polyglycolic Acid chemistry, Serum Albumin chemistry
- Abstract
This study investigates encapsulation efficiency of model drug, encapsulated by magnetic poly d,l-lactic-co-glycolic acid (PLGA) nanoparticles (NPs). This is the following part of our preceding paper, which is referred in this paper as Part I. Magnetic nanoparticles and model drug human serum albumin (HSA)-loaded PLGA NPs were prepared by the double emulsion solvent evaporation method. Among five important process variables, concentration of PLGA and concentration of HSA in the inner aqueous phase along with their cross-effect had the strongest influence on the encapsulation efficiency. Encapsulation efficiency of nanoparticles ranged from 18% to 97% depending on the process conditions. Higher encapsulation efficiencies can be achieved by using low HSA and high PLGA concentrations. The optimization process, carried out by exact mathematical tools using GAMSTM/MINOS software makes it easier to find out optimum process conditions to achieve comparatively high encapsulation efficiency (e.g. 92.3%) for relatively small-sized PLGA NPs (e.g. 155 nm).
- Published
- 2014
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6. Fluorescent Nanodiamonds with Bioorthogonally Reactive Protein-Resistant Polymeric Coatings.
- Author
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Rehor I, Mackova H, Filippov SK, Kucka J, Proks V, Slegerova J, Turner S, Van Tendeloo G, Ledvina M, Hruby M, and Cigler P
- Abstract
The novel synthesis of a polymeric interface grown from the surface of bright fluorescent nanodiamonds is reported. The polymer enables bioorthogonal attachment of various molecules by click chemistry; the particles are resistant to nonspecific protein adsorption and show outstanding colloidal stability in buffers and biological media. The coating fully preserves the unique optical properties of the nitrogen-vacancy centers that are crucial for bioimaging and sensoric applications., (Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2014
- Full Text
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7. Co-encapsulation of human serum albumin and superparamagnetic iron oxide in PLGA nanoparticles: part I. Effect of process variables on the mean size.
- Author
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Shubhra QT, Kardos AF, Feczkó T, Mackova H, Horák D, Tóth J, Dósa G, and Gyenis J
- Subjects
- Humans, Nanoparticles ultrastructure, Particle Size, Polylactic Acid-Polyglycolic Acid Copolymer, Ferric Compounds chemistry, Lactic Acid chemistry, Nanoparticles chemistry, Polyglycolic Acid chemistry, Serum Albumin chemistry
- Abstract
PLGA (poly d,l-lactic-co-glycolic acid) nanoparticles (NPs) encapsulating magnetite nanoparticles (MNPs) along with a model drug human serum albumin (HSA) were prepared by double emulsion solvent evaporation method. This Part I will focus on size and size distribution of prepared NPs, whereas encapsulation efficiency will be discussed in Part II. It was found that mean hydrodynamic particle size was influenced by five important process variables. To explore their effects, a five-factorial, three-level experimental design and statistical analysis were carried out using STATISTICA® software. Effect of process variables on the mean size of nanoparticles was investigated and finally conditions to minimize size of NPs were proposed. GAMS™/MINOS software was used for optimization. The mean hydrodynamic size of nanoparticles ranged from 115 to 329 nm depending on the process conditions. Smallest possible mean particle size can be achieved by using low polymer concentration and high dispersion energy (enough sonication time) along with small aqueous/organic volume ratio.
- Published
- 2014
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8. Thermoresponsive micelles for radionuclide delivery.
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Hruby M, Filippov SK, Panek J, Novakova M, Mackova H, Kucka J, and Ulbrich K
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- Temperature, Drug Delivery Systems, Micelles, Polymers administration & dosage, Radioisotopes administration & dosage
- Published
- 2010
- Full Text
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9. Polyoxazoline thermoresponsive micelles as radionuclide delivery systems.
- Author
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Hruby M, Filippov SK, Panek J, Novakova M, Mackova H, Kucka J, Vetvicka D, and Ulbrich K
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- Hemolysis, Humans, Magnetic Resonance Spectroscopy, Molecular Weight, Temperature, Micelles, Oxazoles chemistry, Polymers chemistry, Radioisotopes administration & dosage
- Abstract
Thermoresponsive polymer micelles are promising drug and radionuclide carriers with a strong passive targeting effect into solid tumors. We have synthesized ABA triblock copolymers poly[2-methyl-2-oxazoline-block-(2-isopropyl-2-oxazoline-co-2-butyl-2-oxazoline)-block-2-methyl-2-oxazoline]. These polymers are molecularly dissolved in aqueous millieu below the cloud point temperature (CPT) of the thermoresponsive central block and above CPT form polymer micelles at CMC 5-10 × 10(-5) g · mL(-1) with diameter ≈200 nm. The phenolic moiety introduced into the copolymer allowed radionuclide labeling with iodine-125 ongoing in good yield with sufficient in vitro stability under model conditions.
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- 2010
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10. New coupling strategy for radionuclide labeling of synthetic polymers.
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Hruby M, Kucka J, Novakova M, Mackova H, and Vetrik M
- Subjects
- Azo Compounds chemistry, Isotope Labeling methods, Polymers chemistry, Radioisotopes chemistry
- Abstract
We have developed a radiolabeling strategy for synthetic polymers based on the formation of azo dye usable for both covalent and chelating labeling modalities under mild conditions. Poly[N-(2-hydroxypropyl)methacrylamide] and poly(N-isopropyl acrylamide) were used as model polymers. N-methacryloyl tyrosinamide was introduced into the polymers and the phenolic moiety was then reacted with diazotized chelator precursors. The conjugates were radiolabeled with both the covalently bound (iodine-125) and chelated (indium-111) radionuclides in high yields and sufficient in vitro stability of the labels was proven., (Copyright (c) 2009 Elsevier Ltd. All rights reserved.)
- Published
- 2010
- Full Text
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11. Thermoresponsive, hydrolytically degradable polymer micelles intended for radionuclide delivery.
- Author
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Hruby M, Konak C, Kucka J, Vetrik M, Filippov SK, Vetvicka D, Mackova H, Karlsson G, Edwards K, Rihova B, and Ulbrich K
- Subjects
- Acrylamides chemistry, Acrylamides metabolism, Acrylic Resins, Biocompatible Materials chemistry, Biocompatible Materials metabolism, Drug Delivery Systems, Humans, Hydrolysis, Materials Testing, Molecular Structure, Radioisotopes chemistry, Drug Carriers chemical synthesis, Drug Carriers chemistry, Drug Carriers metabolism, Micelles, Polymers chemical synthesis, Polymers chemistry, Polymers metabolism, Radioisotopes metabolism, Temperature
- Abstract
Novel polymer micelles, prepared by self-assembling thermoresponsive poly(N-isopropylacrylamide)-graft-poly[N-(2-hydroxypropyl)methacrylamide] copolymers with hydrolytically degradable N-glycosylamine groups between the polymer blocks are proposed for delivery of diagnostic and therapeutic radionuclides into solid tumors. The micelles are formed by fast heating of an aqueous solution of the copolymer to 37 degrees C. They have a hydrodynamic diameter of 128 nm (measured using dynamic light scattering) and slowly degrade during incubation in aqueous buffer at pH = 7.4. Labeling with both (131)I and (90)Y proceeds with high yields (>85%). The unlabeled polymers are not cytotoxic for any of the tested murine and human cell lines.
- Published
- 2009
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12. New bioerodable thermoresponsive polymers for possible radiotherapeutic applications.
- Author
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Hruby M, Kucka J, Lebeda O, Mackova H, Babic M, Konak C, Studenovsky M, Sikora A, Kozempel J, and Ulbrich K
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- Biotransformation, Body Temperature, Drug Delivery Systems trends, Polymers administration & dosage, Radiotherapy trends, Drug Delivery Systems methods, Polymers chemistry, Polymers metabolism, Radiotherapy methods, Temperature
- Abstract
A new thermoresponsive system designed for local radiotherapy has been developed. In this system a radionuclide complex is entrapped in a thermoresponsive polymer locally precipitated at body temperature after injection of a polymer-complex solution into the tissue where a therapeutic effect is required. The lifetime of the system is controlled by the rate of polymer hydrolysis, its dissolution and elimination from the body. The thermoresponsive polymer with the cloud temperature (CT) below body temperature is based on copolymers of N-isopropylmethacrylamide with a methacrylamide-type comonomer containing hydrophobic n-alkyls of three different sizes (C(3), C(6) and C(12)) bonded by a hydrolytically labile hydrazone bond. Hydrolysis of hydrazone bond results in a copolymer soluble at body temperature. The copolymer containing 27.5 mole% of the comonomer with the C(6) moiety, which was chosen for further study, has the CT 22 degrees C and its phase separation is complete at 34 degrees C. Polymer dissolution is complete within 48 h at both pH 5.0 or 7.4. The model therapeutic radionuclide, (64)Cu, in the form of its hydrophobic chelate bis(quinolin-8-olato-N,O) [(64)Cu]copper, is efficiently kept hydrophobically entrapped in the phase-separated polymer until the dissolution by hydrolytic degradation is completed.
- Published
- 2007
- Full Text
- View/download PDF
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