1. jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths
- Author
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Mohr, Manuel Alexander, Bushey, Daniel, Aggarwal, Abhi, Marvin, Jonathan S, Kim, Jeong Jun, Marquez, Emiliano Jimenez, Liang, Yajie, Patel, Ronak, Macklin, John J, Lee, Chi-Yu, Tsang, Arthur, Tsegaye, Getahun, Ahrens, Allison M, Chen, Jerry L, Kim, Douglas S, Wong, Allan M, Looger, Loren L, Schreiter, Eric R, and Podgorski, Kaspar
- Subjects
Biological Sciences ,Biotechnology ,Generic health relevance ,Animals ,Calcium ,Drosophila ,Female ,Fluorescent Dyes ,Lasers ,Male ,Mice ,Mice ,Inbred C57BL ,Microscopy ,Fluorescence ,Multiphoton ,Molecular Imaging ,Somatosensory Cortex ,Technology ,Medical and Health Sciences ,Developmental Biology ,Biological sciences - Abstract
Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.
- Published
- 2020