9 results on '"Mackenzie A. Catron"'
Search Results
2. Subclinical β‐amyloid peptides impair homeostatic synaptic potentiation in hippocampal neurons in 5XFAD mice
- Author
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Mackenzie A. Catron, Rachel K. Howe, Emily K. St. John, Cobie Victoria Potesta, Alena Shostak, Matthew Schrag, and Chengwen Zhou
- Subjects
Psychiatry and Mental health ,Cellular and Molecular Neuroscience ,Developmental Neuroscience ,Epidemiology ,Health Policy ,Neurology (clinical) ,Geriatrics and Gerontology - Published
- 2022
3. Sleep slow-wave oscillations trigger seizures in a genetic epilepsy model of Dravet syndrome
- Author
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Mackenzie A Catron, Rachel K Howe, Gai-Linn K Besing, Emily K St. John, Cobie Victoria Potesta, Martin J Gallagher, Robert L Macdonald, and Chengwen Zhou
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Cellular and Molecular Neuroscience ,Psychiatry and Mental health ,Neurology ,Biological Psychiatry - Abstract
Sleep is the preferential period when epileptic spike–wave discharges appear in human epileptic patients, including genetic epileptic seizures such as Dravet syndrome with multiple mutations including SCN1A mutation and GABAA receptor γ2 subunit Gabrg2Q390X mutation in patients, which presents more severe epileptic symptoms in female patients than male patients. However, the seizure onset mechanism during sleep still remains unknown. Our previous work has shown that the sleep-like state-dependent homeostatic synaptic potentiation can trigger epileptic spike–wave discharges in one transgenic heterozygous Gabrg2+/Q390X knock-in mouse model.1 Here, using this heterozygous knock-in mouse model, we hypothesized that slow-wave oscillations themselves in vivo could trigger epileptic seizures. We found that epileptic spike–wave discharges in heterozygous Gabrg2+/Q390X knock-in mice exhibited preferential incidence during non-rapid eye movement sleep period, accompanied by motor immobility/facial myoclonus/vibrissal twitching and more frequent spike–wave discharge incidence appeared in female heterozygous knock-in mice than male heterozygous knock-in mice. Optogenetically induced slow-wave oscillations in vivo significantly increased epileptic spike–wave discharge incidence in heterozygous Gabrg2+/Q390X knock-in mice with longer duration of non-rapid eye movement sleep or quiet–wakeful states. Furthermore, suppression of slow-wave oscillation-related homeostatic synaptic potentiation by 4-(diethylamino)-benzaldehyde injection (i.p.) greatly attenuated spike–wave discharge incidence in heterozygous knock-in mice, suggesting that slow-wave oscillations in vivo did trigger seizure activity in heterozygous knock-in mice. Meanwhile, sleep spindle generation in wild-type littermates and heterozygous Gabrg2+/Q390X knock-in mice involved the slow-wave oscillation-related homeostatic synaptic potentiation that also contributed to epileptic spike–wave discharge generation in heterozygous Gabrg2+/Q390X knock-in mice. In addition, EEG spectral power of delta frequency (0.1–4 Hz) during non-rapid eye movement sleep was significantly larger in female heterozygous Gabrg2+/Q390X knock-in mice than that in male heterozygous Gabrg2+/Q390X knock-in mice, which likely contributes to the gender difference in seizure incidence during non-rapid eye movement sleep/quiet–wake states of human patients. Overall, all these results indicate that slow-wave oscillations in vivo trigger the seizure onset in heterozygous Gabrg2+/Q390X knock-in mice, preferentially during non-rapid eye movement sleep period and likely generate the sex difference in seizure incidence between male and female heterozygous Gabrg2+/Q390X knock-in mice.
- Published
- 2022
4. Dravet syndrome-associated mutations in GABRA1, GABRB2 and GABRG2 define the genetic landscape of defects of GABAA receptors
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Yuwu Jiang, Wangzhen Shen, Jiaoyang Chen, Robert L. Macdonald, Ningning Hu, Yuehua Zhang, Xiaojuan Tian, Ying Yang, Ciria C. Hernandez, and Mackenzie A. Catron
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0301 basic medicine ,Protein subunit ,GABRG2 ,Pathogenesis ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,GABRB2 ,0302 clinical medicine ,Dravet syndrome ,PIP2 ,medicine ,Receptor ,Gene ,Dravet syndrome-associated mutations ,Biological Psychiatry ,Genetics ,biology ,GABAA receptor ,AcademicSubjects/SCI01870 ,medicine.disease ,Phenotype ,Psychiatry and Mental health ,GABRA1 ,030104 developmental biology ,Neurology ,biology.protein ,Original Article ,AcademicSubjects/MED00310 ,030217 neurology & neurosurgery - Abstract
Dravet syndrome is a rare, catastrophic epileptic encephalopathy that begins in the first year of life, usually with febrile or afebrile hemiclonic or generalized tonic–clonic seizures followed by status epilepticus. De novo variants in genes that mediate synaptic transmission such as SCN1A and PCDH19 are often associated with Dravet syndrome. Recently, GABAA receptor subunit genes (GABRs) encoding α1 (GABRA1), β3 (GABRB3) and γ2 (GABRG2), but not β2 (GABRB2) or β1 (GABRB1), subunits are frequently associated with Dravet syndrome or Dravet syndrome-like phenotype. We performed next generation sequencing on 870 patients with Dravet syndrome and identified nine variants in three different GABRs. Interestingly, the variants were all in genes encoding the most common GABAA receptor, the α1β2γ2 receptor. Mutations in GABRA1 (c.644T>C, p. L215P; c.640C>T, p. R214C; c.859G>A; V287I; c.641G>A, p. R214H) and GABRG2 (c.269C>G, p. T90R; c.1025C>T, p. P342L) presented as de novo cases, while in GABRB2 two variants were de novo (c.992T>C, p. F331S; c.542A>T, p. Y181F) and one was autosomal dominant and inherited from the maternal side (c.990_992del, p.330_331del). We characterized the effects of these GABR variants on GABAA receptor biogenesis and channel function. We found that defects in receptor gating were the common deficiency of GABRA1 and GABRB2 Dravet syndrome variants, while mainly trafficking defects were found with the GABRG2 (c.269C>G, p. T90R) variant. It seems that variants in α1 and β2 subunits are less tolerated than in γ2 subunits, since variant α1 and β2 subunits express well but were functionally deficient. This suggests that all of these GABR variants are all targeting GABR genes that encode the assembled α1β2γ2 receptor, and regardless of which of the three subunits are mutated, variants in genes coding for α1, β2 and γ2 receptor subunits make them candidate causative genes in the pathogenesis of Dravet syndrome., Next-generation sequencing on 870 patients with Dravet syndrome identified nine variants in GABRA1, GABRB2 and GABRG2 genes, which encode the most common α1β2γ2 GABAA receptor in the CNS. Hernandez et al. report that mutations in multiple genes (GABRA1, GABRB2 and GABRG2) have a common target (α1β2γ2) to cause Dravet syndrome., Graphical Abstract Graphical Abstract
- Published
- 2021
5. Impaired State-Dependent Potentiation of GABAergic Synaptic Currents Triggers Seizures in a Genetic Generalized Epilepsy Model
- Author
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Li Ding, Caitlyn M. Hanna, Chun-Qing Zhang, Martin J. Gallagher, Mackenzie A Catron, Chengwen Zhou, and Robert L. Macdonald
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0303 health sciences ,Chemistry ,Cognitive Neuroscience ,Long-term potentiation ,Optogenetics ,Inhibitory postsynaptic potential ,Neuronal action potential ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,Synaptic plasticity ,Epilepsy syndromes ,Excitatory postsynaptic potential ,GABAergic ,Original Article ,Neuroscience ,030217 neurology & neurosurgery ,030304 developmental biology - Abstract
Epileptic activity in genetic generalized epilepsy (GGE) patients preferentially appears during sleep and its mechanism remains unknown. Here, we found that sleep-like slow-wave oscillations (0.5 Hz SWOs) potentiated excitatory and inhibitory synaptic currents in layer V cortical pyramidal neurons from wild-type (wt) mouse brain slices. In contrast, SWOs potentiated excitatory, but not inhibitory, currents in cortical neurons from a heterozygous (het) knock-in (KI) Gabrg2+Q/390X model of Dravet epilepsy syndrome. This created an imbalance between evoked excitatory and inhibitory currents to effectively prompt neuronal action potential firings. Similarly, physiologically similar up-/down-state induction (present during slow-wave sleep) in cortical neurons also potentiated excitatory synaptic currents within brain slices from wt and het KI mice. Moreover, this state-dependent potentiation of excitatory synaptic currents entailed some signaling pathways of homeostatic synaptic plasticity. Consequently, in het KI mice, in vivo SWO induction (using optogenetic methods) triggered generalized epileptic spike-wave discharges (SWDs), being accompanied by sudden immobility, facial myoclonus, and vibrissa twitching. In contrast, in wt littermates, SWO induction did not cause epileptic SWDs and motor behaviors. To our knowledge, this is the first mechanism to explain why epileptic SWDs preferentially happen during non rapid eye-movement sleep and quiet-wakefulness in human GGE patients.
- Published
- 2020
6. Impaired state-dependent potentiation of GABAergic synaptic currents triggers seizures in an idiopathic generalized epilepsy model
- Author
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Chun-Qing Zhang, Caitlyn M. Hanna, Martin J. Gallagher, Mackenzie A. Catron, Li Ding, Robert L. Macdondald, and Chengwen Zhou
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0303 health sciences ,Chemistry ,GABAA receptor ,Long-term potentiation ,Optogenetics ,Inhibitory postsynaptic potential ,medicine.disease ,Idiopathic generalized epilepsy ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Excitatory postsynaptic potential ,GABAergic ,Neuroscience ,030217 neurology & neurosurgery ,Ex vivo ,030304 developmental biology - Abstract
Idiopathic generalized epilepsy(IGE) patients have genetic causes and their seizure onset mechanisms particularly during sleep remain elusive. Here we proposed that sleep-like slow-wave oscillations(0.5 Hz SWOs) potentiated excitatory or inhibitory synaptic currents in layer V cortical pyramidal neurons from wild-type(wt) mouse ex vivo brain slices. In contrast, SWOs potentiated excitatory, not inhibitory, currents in cortical neurons from heterozygous(het) knock-in(KI) IGE mice(GABAA receptor γ2 subunit Gabrg2Q390X mutation), creating an imbalance between evoked excitatory and inhibitory currents to effectively prompt neuronal action potentials. Similarly, more physiologically similar up/down-state(present during slow-wave sleep) induction in cortical neurons could potentiate excitatory synaptic currents within slices from wt/het Gabrg2Q390X KI mice. Consequently, SWOs or up/down-state induction in vivo (using optogenetic method) could trigger epileptic spike-wave discharges(SWDs) in het Gabrg2Q390X KI mice. To our knowledge, this is the first operative mechanism to explain why epileptic SWDs preferentially happen during non-REM sleep or quiet-wakefulness in human IGE patients.
- Published
- 2020
7. GABAA receptor β3 subunit mutation D120N causes Lennox–Gastaut syndrome in knock-in mice
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Robert L. Macdonald, Chengwen Zhou, Shimian Qu, Mackenzie A. Catron, Wangzhen Shen, Vaishali S. Janve, and Rachel K Howe
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0301 basic medicine ,medicine.medical_specialty ,genetic structures ,Atypical absence seizures ,Congenic ,early-onset epileptic encephalopathy ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Gene knockin ,GABRB3 ,medicine ,Receptor ,seizures ,GABAA receptor ,Seizure types ,business.industry ,General Engineering ,medicine.disease ,030104 developmental biology ,Endocrinology ,GABAergic ,Original Article ,genetic epilepsies ,business ,Lennox–Gastaut syndrome ,030217 neurology & neurosurgery - Abstract
The Lennox–Gastaut syndrome is a devastating early-onset epileptic encephalopathy, associated with severe behavioural abnormalities. Its pathophysiology, however, is largely unknown. A de novo mutation (c.G358A, p.D120N) in the human GABA type-A receptor β3 subunit gene (GABRB3) has been identified in a patient with Lennox–Gastaut syndrome. To determine whether the mutation causes Lennox–Gastaut syndrome in vivo in mice and to elucidate its mechanistic effects, we generated the heterozygous Gabrb3+/D120N knock-in mouse and found that it had frequent spontaneous atypical absence seizures, as well as less frequent tonic, myoclonic, atonic and generalized tonic–clonic seizures. Each of these seizure types had a unique and characteristic ictal EEG. In addition, knock-in mice displayed abnormal behaviours seen in patients with Lennox–Gastaut syndrome including impaired learning and memory, hyperactivity, impaired social interactions and increased anxiety. This Gabrb3 mutation did not alter GABA type-A receptor trafficking or expression in knock-in mice. However, cortical neurons in thalamocortical slices from knock-in mice had reduced miniature inhibitory post-synaptic current amplitude and prolonged spontaneous thalamocortical oscillations. Thus, the Gabrb3+/D120N knock-in mouse recapitulated human Lennox–Gastaut syndrome seizure types and behavioural abnormalities and was caused by impaired inhibitory GABAergic signalling in the thalamocortical loop. In addition, treatment with antiepileptic drugs and cannabinoids ameliorated atypical absence seizures in knock-in mice. This congenic knock-in mouse demonstrates that a single-point mutation in a single gene can cause development of multiple types of seizures and multiple behavioural abnormalities. The knock-in mouse will be useful for further investigation of the mechanisms of Lennox–Gastaut syndrome development and for the development of new antiepileptic drugs and treatments., We generated the Gabrb3+/D120N knock-in mouse harbouring a mutation in the GABAAR β3 subunit identified in an epileptic patient with Lennox–Gastaut syndrome. This mouse displays the full triad of features that characterize Lennox–Gastaut syndrome: multiple seizure semiologies, generalized slow spike-and-wave discharge on EEG and cognitive dysfunction and behavioural abnormalities., Graphical Abstract Graphical Abstract
- Published
- 2020
8. Single Quantum Dot Tracking Reveals Serotonin Transporter Diffusion Dynamics are Correlated with Cholesterol-Sensitive Threonine 276 Phosphorylation Status in Primary Midbrain Neurons
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Mackenzie A Catron, Qi Zhang, Robert L. Macdonald, Sandra J. Rosenthal, Danielle M. Bailey, and Oleg Kovtun
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Threonine ,0301 basic medicine ,Physiology ,Cognitive Neuroscience ,Endogeny ,Biochemistry ,Midbrain ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Mesencephalon ,Quantum Dots ,Cyclic GMP-Dependent Protein Kinases ,Animals ,Phosphorylation ,Neurotransmitter ,Serotonin transporter ,Neurons ,biology ,Chemistry ,Cholesterol ,Cell Membrane ,beta-Cyclodextrins ,RNA-Binding Proteins ,Cell Biology ,General Medicine ,Rats ,Cell biology ,030104 developmental biology ,biology.protein ,Serotonin ,Hydroxymethylglutaryl-CoA Reductase Inhibitors ,030217 neurology & neurosurgery - Abstract
Serotonin transporter (SERT) terminates serotonin signaling in the brain by enabling rapid clearance of the neurotransmitter. SERT dysfunction has been associated with a variety of psychiatric disorders, including depression, anxiety, and autism. Visualizing SERT behavior at the single molecule level in endogenous systems remains a challenge. In this study, we utilize quantum dot (QD) single particle tracking (SPT) to capture SERT dynamics in primary rat midbrain neurons. Membrane microenvironment, specifically membrane cholesterol, plays a key role in SERT regulation and has been found to affect SERT conformational state. We sought to determine how reduced cholesterol content affects both lateral mobility and phosphorylation of conformationally sensitive threonine 276 (Thr276) in endogenous SERT using two different methods of cholesterol manipulation, statins and methyl-β-cyclodextrin. Both chronic and acute cholesterol depletion increased SERT lateral diffusion, radial displacement along the membrane, mobile fraction, and Thr276 phosphorylation levels. Overall, this work has provided new insights about endogenous neuronal SERT mobility and its associations with membrane cholesterol and SERT phosphorylation status.
- Published
- 2018
9. The K328M substitution in the human GABAA receptor gamma2 subunit causes GEFS+ and premature sudden death in knock-in mice
- Author
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Shimian Qu, Robert L. Macdonald, Mackenzie A. Catron, Chengwen Zhou, Xuan Huang, Rachel Howe, Wangzhen Shen, and Ningning Hu
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SUDEP ,GABAA receptor ,Protein subunit ,Substitution (logic) ,Biology ,Sudden death ,Generalized epilepsy with febrile seizures ,lcsh:RC321-571 ,Cell biology ,Neurology ,Genetic epilepsies ,Gene knockin ,GABRG2 gene ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry - Published
- 2021
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