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20. Human immune response against outer membrane proteins of Moraxella (Branhamella) catarrhalis determined by immunoblotting and enzyme immunoassay.

Author

Helminen, M E, Beach, R, Maciver, I, Jarosik, G, Hansen, E J, and Leinonen, M

Abstract

The role of Moraxella (Branhamella) catarrhalis as a respiratory tract pathogen is increasingly recognized. We looked at the human immune response against individual outer membrane proteins of M. catarrhalis and against the 81-kDa CopB protein, which has previously been shown to be a target for protective antibodies. Paired serum samples from six elderly patients with pneumonia were tested by Western blot (immunoblot) analysis by using outer membrane vesicles of M. catarrhalis 035E as antigen. All of the six convalescent-phase serum samples reacted with a protein which migrated at the position of the CopB protein and with a high-molecular-weight protein of M. catarrhalis; three serum samples also reacted with a 34-kDa outer membrane protein. Paired serum samples from 18 patients, 10 of which had M. catarrhalis infection on the basis of previous serology results, were tested by enzyme immunoassay (EIA) with the CopB protein and whole cells of M. catarrhalis 035E as antigens. Nine patients showed a significant rise in EIA titer between acute- and convalescent-phase sera when whole bacterial cells were used as antigens. Six (67%) patient samples that were positive by the EIA with the whole-cell antigen were also positive by the EIA with the CopB antigen, and six of nine patient samples negative by the EIA with the whole-cell antigen were also negative by the EIA with the CopB antigen. These results suggest that both the CopB and a high-molecular-weight protein are major targets of the immune response against M. catarrhalis, and further studies with greater amounts of patient materials are needed to elucidate the usefulness of CopB as an antigen in etiologic studies.

Published
1995

25. The influence of hydration and heating on visualisation of nailfold capillaries in patients with systemic sclerosis.

Author

Sheikh ASF, Wilkinson J, MacIver I, Manning J, Murray A, Dinsdale G, and Herrick AL

Subjects
Adult, Aged, Drinking, Female, Humans, Male, Middle Aged, Predictive Value of Tests, Reproducibility of Results, Young Adult, Capillaries diagnostic imaging, Hyperthermia, Induced, Microscopic Angioscopy, Nails blood supply, Organism Hydration Status, Scleroderma, Systemic diagnostic imaging
Published
2021
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26. Human immune response against outer membrane proteins of Moraxella (Branhamella) catarrhalis determined by immunoblotting and enzyme immunoassay.

Author

Helminen ME, Beach R, Maciver I, Jarosik G, Hansen EJ, and Leinonen M

Subjects
Aged, Antibodies, Bacterial blood, Antibodies, Bacterial immunology, Blotting, Western, Humans, Immunodominant Epitopes immunology, Immunoenzyme Techniques, Moraxella catarrhalis isolation & purification, Neisseriaceae Infections blood, Neisseriaceae Infections microbiology, Pneumonia, Bacterial blood, Pneumonia, Bacterial microbiology, Sputum microbiology, Antibodies, Bacterial biosynthesis, Antigens, Bacterial immunology, Bacterial Outer Membrane Proteins immunology, Moraxella catarrhalis immunology, Neisseriaceae Infections immunology, Pneumonia, Bacterial immunology
Abstract

The role of Moraxella (Branhamella) catarrhalis as a respiratory tract pathogen is increasingly recognized. We looked at the human immune response against individual outer membrane proteins of M. catarrhalis and against the 81-kDa CopB protein, which has previously been shown to be a target for protective antibodies. Paired serum samples from six elderly patients with pneumonia were tested by Western blot (immunoblot) analysis by using outer membrane vesicles of M. catarrhalis 035E as antigen. All of the six convalescent-phase serum samples reacted with a protein which migrated at the position of the CopB protein and with a high-molecular-weight protein of M. catarrhalis; three serum samples also reacted with a 34-kDa outer membrane protein. Paired serum samples from 18 patients, 10 of which had M. catarrhalis infection on the basis of previous serology results, were tested by enzyme immunoassay (EIA) with the CopB protein and whole cells of M. catarrhalis 035E as antigens. Nine patients showed a significant rise in EIA titer between acute- and convalescent-phase sera when whole bacterial cells were used as antigens. Six (67%) patient samples that were positive by the EIA with the whole-cell antigen were also positive by the EIA with the CopB antigen, and six of nine patient samples negative by the EIA with the whole-cell antigen were also negative by the EIA with the CopB antigen. These results suggest that both the CopB and a high-molecular-weight protein are major targets of the immune response against M. catarrhalis, and further studies with greater amounts of patient materials are needed to elucidate the usefulness of CopB as an antigen in etiologic studies.

Published
1995
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27. Rat model of chronic lung infections caused by non-typable Haemophilus influenzae.

Author

Maciver I, Silverman SH, Brown MR, and O'Reilly T

Subjects
Animals, Antibodies, Bacterial biosynthesis, Antibody Specificity, Bacterial Outer Membrane Proteins immunology, Chronic Disease, Female, Haemophilus Infections drug therapy, Haemophilus Infections immunology, Lipopolysaccharides immunology, Microbial Sensitivity Tests, Pneumonia drug therapy, Pneumonia immunology, Rats, Rats, Inbred Strains, Weight Gain, Disease Models, Animal, Haemophilus Infections microbiology, Pneumonia microbiology
Abstract

Patients with chronic obstructive pulmonary disease (COPD) often have chronic or recurrent pulmonary infections with non-typable Haemophilus influenzae. A model of these infections exploited agar bead vehicles to protect the inoculum from rapid clearance, and a chronic lung infection of at least 42 days duration was established in rats. This infection induced increases in serum IgG titres to outer-membrane (OM) and lipo-oligosaccharide (LOS) antigens; immunoblotting demonstrated that this humoral response was directed partly against the outer-membrane proteins (OMPs). Lung lavage fluid also contained an increased titre of IgG antibodies to OM and LOS 42 days after infection. Antibodies produced during infection with one strain of H. influenzae cross-reacted with OMPs from another, non-typable H. influenzae strain. Despite their encasement in agar beads, pulmonary H. influenzae remained susceptible to amoxycillin. This model of chronic pulmonary infections due to non-typable H. influenzae appears to resemble the situation in COPD patients and may be useful for experimental therapeutic studies.

Published
1991
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