10 results on '"Maathuis, Annet J. H."'
Search Results
2. Profiling human gut bacterial metabolism and its kinetics using [U-C-13]glucose and NMR
- Author
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de Graaf, Albert A., Maathuis, Annet J. H., de Waard, Pieter, Deutz, Nicolaas E. P., Dijkema, Cor, de Vos, Willem M., Venema, Koen, Surgery, and RS: NUTRIM School of Nutrition and Translational Research in Metabolism
- Subjects
metabolic flux analysis ,bacterial metabolism ,stable isotopes ,metabolomics ,NMR - Abstract
This study introduces a stable-isotope metabolic approach employing [U-C-13]glucose that, as a novelty, allows selective profiling of the human intestinal microbial metabolic products of carbohydrate food components, as well as the measurement of the kinetics of their formation pathways, in a single experiment. A well-established, validated in vitro model of human intestinal fermentation was inoculated with standardized gastrointestinal microbiota from volunteers. After culture stabilization, [U-C-13]glucose was added as an isotopically labeled metabolic precursor. System lumen and dialysate samples were taken at regular intervals. Metabolite concentrations and isotopic labeling were determined by NMR, GC, and enzymatic methods. The main microbial metabolites were lactate, acetate, butyrate, formate, ethanol, and glycerol. They together accounted for a C-13 recovery rate as high as 91.2%. Using an NMR chemical shift prediction approach, several minor products that showed C-13 incorporation were identified as organic acids, amino acids, and various alcohols. Using computer modeling of the C-12 contents and C-13 labeling kinetics, the metabolic fluxes in the gut microbial pathways for synthesis of lactate, formate, acetate, and butyrate were determined separately for glucose and unlabeled background substrates. This novel approach enables the study of the modulation of human intestinal function by single nutrients, providing a new rational basis for achieving control of the short-chain fatty acids profile by manipulating substrate and microbiota composition in a purposeful manner.
- Published
- 2010
3. Dose-Dependent Prebiotic Effect of Lactulose in a Computer-Controlled In Vitro Model of the Human Large Intestine.
- Author
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Bothe, Melanie K., Maathuis, Annet J. H., Bellmann, Susann, van der Vossen, Jos M. B. M., Berressem, Dirk, Koehler, Annalena, Schwejda-Guettes, Susann, Gaigg, Barbara, Kuchinka-Koch, Angelika, and Stover, John F.
- Abstract
Lactulose, a disaccharide of galactose and fructose, used as a laxative or ammonia-lowering drug and as a functional food ingredient, enhances growth of Bifidobacterium and Lactobacillus at clinically relevant dosages. The prebiotic effect of subclinical dosages of Lactulose, however, remains to be elucidated. This study analyses changes in the microbiota and their metabolites after a 5 days Lactulose treatment using the TIM-2 system, a computer-controlled model of the proximal large intestine representing a complex, high density, metabolically active, anaerobic microbiota of human origin. Subclinical dosages of 2-5 g Lactulose were used. While 2 g Lactulose already increased the short-chain fatty acid levels of the intestinal content, 5 g Lactulose were required daily for 5 days in this study to exert the full beneficial prebiotic effect consisting of higher bacterial counts of Bifidobacterium, Lactobacillus, and Anaerostipes, a rise in acetate, butyrate and lactate, as well as a decrease in branched-chain fatty acids, pH (suggested by an increase in NaOH usage), and ammonia. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
4. Metabolite Production during in Vitro Colonic Fermentation of Dietary Fiber: Analysis and Comparison of Two European Diets
- Author
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Tabernero, Maria, primary, Venema, Koen, additional, Maathuis, Annet J. H., additional, and Saura-Calixto, Fulgencio D., additional
- Published
- 2011
- Full Text
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5. Effect of the Novel Polysaccharide PolyGlycopleX® on Short-Chain Fatty Acid Production in a Computer-Controlled in Vitro Model of the Human Large Intestine.
- Author
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Reimer, Raylene A., Maathuis, Annet J. H., Venema, Koen, Lyon, Michael R., Gahler, Roland J., and Wood, Simon
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- 2014
- Full Text
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6. Galacto-Oligosaccharides Have Prebiotic Activity in a Dynamic in Vitro Colon Model Using a 13C-Labeling Technique.
- Author
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Maathuis, Annet J. H., Van Den Heuvel, Ellen G., Schoterman, Margriet H. C., and Venema, Koen
- Subjects
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OLIGOSACCHARIDES , *CARBON isotopes , *FERMENTATION , *MICROBIAL metabolites , *LIQUID chromatography , *LACTOBACILLUS , *PROBIOTICS - Abstract
Galacto-oligosaccharides (GOS) are considered to be prebiotic, altliough the contribution of specific members of the microbiota to GOS fermentation and the exact microbial metabolites that are produced upon GOS fermentation are largely unknown. We aimed to determine this using uniformly 13C-labeled GOS. The normal (control) medium and unlabeled or 13C-labeled GOS was added to a dynamic, validated, in vitro model of the large-intestine containing an aduit-type microbiota. Liquid-chromatography MS was used to measure the incorporation of C label into metabolites. 16S-rRNA stable isotope probing coupled to a phylogenetic micro-array was used to determine label incorporation in microbial biomass. The priman/ members within the complex microbiota that were directly involved in GOS fermentation were shown to be Bifidobacterium longum, B. bifidum, B. catenulatum, Lactobacillus gasseh, and L. salivarius, in line with the prebiotic effect of GOS, although some other species incorporated label also. GOS fermentation led to an increase in acetate (+49%) and lactate (+23%) compared with the control. Total organic acid production was 8.50 and 7.52 mmol/g of carbohydrate fed for the GOS and control experiments, respectively. At the same time, the cumulative production of putrefactive metabolites (branched-chain fatty acids and ammonia) was reduced by 55%. Cross-feeding of metabolites from primary GOS fermenters to other members of the microbiota was observed. Our findings support a prebiotic role for GOS and its potential to act as a synbiotic in combination with certain probiotic strains. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
7. Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption.
- Author
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Kleinnijenhuis AJ, van Holthoon FL, Maathuis AJH, Vanhoecke B, Prawitt J, Wauquier F, and Wittrant Y
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- Animals, Chromatography, High Pressure Liquid, Collagen administration & dosage, Collagen blood, Collagen chemistry, Humans, Intestinal Absorption, Mass Spectrometry, Protein Hydrolysates administration & dosage, Protein Hydrolysates blood, Protein Hydrolysates chemistry, Proteolysis, Collagen metabolism, Protein Hydrolysates metabolism
- Abstract
Protein hydrolysates are an important part of the human diet. Often, they are prepared from milk, soy, or collagen. In the present study, four different collagen hydrolysates were tested, varying in the average molecular weight and the animal source. Three types of samples, the dissolved start products, in vitro generated dialysates (containing the digested components that are potentially available for small intestinal absorption), and human serum collected after product ingestion, were analyzed using LC-MS to compare the state of the hydrolysates before and after absorption, i.e., uptake into the blood. It was found that the composition of the collagen hydrolysates prior to and after ingestion was highly complex and dynamic, which made it challenging to predefine a strategy for a targeted analysis. Therefore, we implemented a new analytical approach to first map hydrolysate data sets by performing non-targeted LC-MS analysis followed by non-targeted and targeted data analysis. It was shown that the insight gained by following such a top down (data) analytical workflow could be crucial for defining a suitable targeted setup and considering data trends beyond the defined targets. After having defined and performed a limited targeted analysis, it was found that, in our experimental setup, Hyp-Gly and especially Pro-Hyp contributed significantly as carrier to the total Hyp increase in blood after ingestion of collagen hydrolysate. Graphical abstract.
- Published
- 2020
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8. Effect of galactooligosaccharides and Bifidobacterium animalis Bb-12 on growth of Lactobacillus amylovorus DSM 16698, microbial community structure, and metabolite production in an in vitro colonic model set up with human or pig microbiota.
- Author
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Martinez RC, Cardarelli HR, Borst W, Albrecht S, Schols H, Gutiérrez OP, Maathuis AJ, de Melo Franco BD, De Martinis EC, Zoetendal EG, Venema K, Saad SM, and Smidt H
- Subjects
- Animals, Bacteria genetics, Bacteria isolation & purification, Bacteria metabolism, Chromatography, Ion Exchange, Denaturing Gradient Gel Electrophoresis, Fatty Acids, Volatile metabolism, Feces microbiology, Galactose chemistry, Humans, Lactobacillus genetics, Lactobacillus isolation & purification, Metagenome, Oligosaccharides metabolism, Polymerase Chain Reaction, RNA, Ribosomal, 16S genetics, Sus scrofa, Bifidobacterium genetics, Colon microbiology, Lactobacillus acidophilus genetics, Lactobacillus acidophilus growth & development, Oligosaccharides administration & dosage, Prebiotics, Probiotics administration & dosage
- Abstract
A validated in vitro model of the large intestine (TIM-2), set up with human or pig faeces, was used to evaluate the impact of potentially probiotic Lactobacillus amylovorus DSM 16698, administered alone (i), in the presence of prebiotic galactooligosaccharides (GOS) (ii), and co-administered with probiotic Bifidobacterium animalis ssp. lactis Bb-12 (Bb-12) (iii) on GOS degradation, microbial growth (L. amylovorus, lactobacilli, bifidobacteria and total bacteria) and metabolite production. High performance anion exchange chromatography revealed that GOS degradation was more pronounced in TIM-2 inoculated with pig faeces than with human faeces. Denaturing gradient gel electrophoresis profiling of PCR-amplified 16S rRNA genes detected a more complex Lactobacillus spp. community in pig faecal material than in human faecal inoculum. According to 16S rRNA gene-targeted qPCR, GOS stimulated the growth of lactobacilli and bifidobacteria in faecal material from both materials. The cumulative production of short chain fatty acids and ammonia was higher (P < 0.05) for pig than for human faeces. However, lactate accumulation was higher (P < 0.05) in the human model and increased after co-administration with GOS and Bb-12. This study reinforced the notion that differences in microbiota composition between target host organisms need to be considered when animal data are extrapolated to human, as is often done with pre- and probiotic intervention studies., (© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
9. Galacto-oligosaccharides have prebiotic activity in a dynamic in vitro colon model using a (13)C-labeling technique.
- Author
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Maathuis AJ, van den Heuvel EG, Schoterman MH, and Venema K
- Subjects
- Acetic Acid metabolism, Ammonia metabolism, Bifidobacterium metabolism, Carbon Isotopes metabolism, Colon metabolism, Colon microbiology, Fatty Acids biosynthesis, Fermentation, Galactose metabolism, Lactic Acid metabolism, Lactobacillus metabolism, Microarray Analysis, Models, Biological, Oligosaccharides metabolism, Phylogeny, RNA, Ribosomal, 16S, Staining and Labeling, Synbiotics, Bifidobacterium drug effects, Colon drug effects, Galactose pharmacology, Lactobacillus drug effects, Oligosaccharides pharmacology, Prebiotics, Probiotics
- Abstract
Galacto-oligosaccharides (GOS) are considered to be prebiotic, although the contribution of specific members of the microbiota to GOS fermentation and the exact microbial metabolites that are produced upon GOS fermentation are largely unknown. We aimed to determine this using uniformly (13)C-labeled GOS. The normal (control) medium and unlabeled or (13)C-labeled GOS was added to a dynamic, validated, in vitro model of the large-intestine containing an adult-type microbiota. Liquid-chromatography MS was used to measure the incorporation of (13)C label into metabolites. 16S-rRNA stable isotope probing coupled to a phylogenetic micro-array was used to determine label incorporation in microbial biomass. The primary members within the complex microbiota that were directly involved in GOS fermentation were shown to be Bifidobacterium longum, B. bifidum, B. catenulatum, Lactobacillus gasseri, and L. salivarius, in line with the prebiotic effect of GOS, although some other species incorporated (13)C label also. GOS fermentation led to an increase in acetate (+49%) and lactate (+23%) compared with the control. Total organic acid production was 8.50 and 7.52 mmol/g of carbohydrate fed for the GOS and control experiments, respectively. At the same time, the cumulative production of putrefactive metabolites (branched-chain fatty acids and ammonia) was reduced by 55%. Cross-feeding of metabolites from primary GOS fermenters to other members of the microbiota was observed. Our findings support a prebiotic role for GOS and its potential to act as a synbiotic in combination with certain probiotic strains.
- Published
- 2012
- Full Text
- View/download PDF
10. A PCR-based method for identification of bifidobacteria from the human alimentary tract at the species level.
- Author
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Venema K and Maathuis AJ
- Subjects
- Child, DNA, Bacterial analysis, Feces microbiology, Humans, RNA, Ribosomal, 16S analysis, Restriction Mapping, Bifidobacterium genetics, Bifidobacterium isolation & purification, Intestine, Large microbiology, Polymerase Chain Reaction methods
- Abstract
A polymerase chain reaction (PCR)-based method was developed for the identification of isolates of Bifidobacterium at the species level. Using two Bifidobacterium-specific primers directed against the 16S ribosomal gene (Bif164 and Bif662), a PCR product was obtained from the type strains of 12 different bifidobacterial species that have been found in the human alimentary tract. After purification of the PCR products, the DNA was restricted with five different restriction enzymes. The size of the different restriction fragments was used as a fingerprint for the identification of individual bifidobacterial species. The amplified ribosomal DNA restriction analysis method was subsequently used to speciate bifidobacterial isolates from child's feces and from an in vitro model of the large intestine.
- Published
- 2003
- Full Text
- View/download PDF
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