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Jun Xiao, Haibo Yu, Zhongwu MaDepartment of Hepatobiliary Surgery, Wenzhou Central Hospital, Wenzhou 325000, People’s Republic of ChinaBackground: Extensive research has shown that long noncoding RNA (lncRNA) is involved in tumorigenesis, including hepatocellular carcinoma (HCC). The lncRNA LINC00339 was reported to regulate the development of lung cancer or breast cancer. However, whether LINC00339 participates in HCC progression remains unclear. Here, our results showed that LINC00339 was upregulated in HCC.Methods: qRT-PCR and in situ hybridization (ISH) was used to analyze LINC00339 expression in tumor tissues and cell lines. CCK8 and colony formation assays were used to analyze cell proliferation. Transwell assay was used to analyze cell migration and invasion. Xenograft experiment was used to test tumor growth in vivo.Results: LINC00339 overexpression was correlated with an advanced stage, metastasis, and bad prognosis in HCC patients. Functional investigation showed that LINC00339 knockdown significantly suppressed HCC cell proliferation, migration, and invasion. Moreover, decreased LINC00339 expression inhibited HCC growth in vivo. Mechanistically, LINC00339 could interact with miR-1182 to promote SKA1 expression. We also demonstrated that SKA1 acted as an oncogene and SKA1 upregulation reversed the effect of LINC00339 silencing.Conclusion: Our results illustrated that the LINC00339/miR-1182/SKA1 axis plays an essential role in HCC progression.Keywords: LINC00339, miR-1182, SKA1, hepatocellular carcinoma, progression &nbsp

The marine primary producers assimilate the atmospheric CO 2 to form the organic carbon in surface water. The organic carbon then settles down through the water column and is removed from the oceans by final preservation in sediments in the form of petroleum or nature gases. The reconstruction of paleoproductivity will thus improve our understanding of the biological processes in the formation of fossil energy resource and help to locate new sites for future exploration. In this study, biorelated elements P, Cd, Al, Ba, as well as redox sensitive element Mo, were analyzed in the 448 rock samples collected from Permian strata at the Shangsi Section, Guangyuan, Northeast Sichuan in China. On the basis of the Ti content, the nondetrital contents of P, Ca, and Al, denoted as P xs , Cd xs , and Al xs , were calculated and found to coincide with the TOC content throughout the whole section, with some enrichment intervals being found in the middle part of Chihsia Formation, topmost Maokou Formation, and Dalong Formation. This suggests that the biorelated elements could be used as proxies for the paleoproductivity here in this section. Ba xs , a paleoproductivity indicator widely used in the paleoceanography, shows insignificant correlation with TOC, P xs , Cd xs , and Al xs , probably arising from the loss of biological barium in anoxic conditions. Compiled with the data of TOC content and P xs , Cd xs , and Al xs , three episodes of enhanced paleoproductivity were identified in Permian strata including the middle part of Chihsia Formation, topmost Maokou Formation, and Dalong Formation.