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Aluminum toxicity is a major abiotic stress on acidic soils, leading to restricted root growth and reduced plant yield. Long non-coding RNAs are crucial signaling molecules regulating the expression of downstream genes, particularly under abiotic stress conditions. However, the extent to which lncRNAs participate in the response to aluminum (Al) stress in barley remains largely unknown. Here, we conducted RNA sequencing of root samples under aluminum stress and compared the lncRNA transcriptomes of two Tibetan wild barley genotypes, XZ16 (Al-tolerant) and XZ61 (Al-sensitive), as well as the aluminum-tolerant cultivar Dayton. In total, 268 lncRNAs were identified as aluminum-responsive genes on the basis of their differential expression profiles under aluminum treatment. Through target gene prediction analysis, we identified 938 candidate lncRNA-messenger RNA (mRNA) pairs that function in a cis-acting manner. Subsequently, enrichment analysis showed that the genes targeted by aluminum-responsive lncRNAs were involved in diterpenoid biosynthesis, peroxisome function, and starch/sucrose metabolism. Further analysis of genotype differences in the transcriptome led to the identification of 15 aluminum-responsive lncRNAs specifically altered by aluminum stress in XZ16. The RNA sequencing data were further validated by RT-qPCR. The functional roles of lncRNA-mRNA interactions demonstrated that these lncRNAs are involved in the signal transduction of secondary messengers, and a disease resistance protein, such as RPP13-like protein 4, is probably involved in aluminum tolerance in XZ16. The current findings significantly contribute to our understanding of the regulatory roles of lncRNAs in aluminum tolerance and extend our knowledge of their importance in plant responses to aluminum stress. [ABSTRACT FROM AUTHOR]

The current study focuses on identifying the candidate genes of a grain yield QTL from a double haploid population, Westonia × Kauz. The QTL region spans 20 Mbp on the IWGSC whole-genome sequence flank with 90K SNP markers. The IWGSC gene annotation revealed 16 high-confidence genes and 41 low-confidence genes. Bioinformatic approaches, including functional gene annotation, ontology investigation, pathway exploration, and gene network study using publicly available gene expression data, enabled the short-listing of four genes for further confirmation. Complete sequencing of those four genes demonstrated that only two genes are polymorphic between the parental cultivars, which are the ferredoxin-like protein gene and the tetratricopeptide-repeat (TPR) protein gene. The two genes were selected for downstream investigation. Two SNP variations were observed in the exon for both genes, with one SNP resulting in changes in amino acid sequence. qPCR-based gene expression showed that both genes were highly expressed in the high-yielding double haploid lines along with the parental cultivar Westonia. In contrast, their expression was significantly lower in the low-yielding lines in the other parent. It can be concluded that these two genes are the contributing genes to the grain yield QTL. [ABSTRACT FROM AUTHOR]

Phenotypic plasticity is the ability of an individual genotype to express phenotype variably in different environments. This study investigated the plasticity of yield-related traits of bread wheat by utilising 225 doubled haploid (DH) lines developed from cv. Westonia and cv. Kauz, through two field trials in Western Australia. Plasticity was quantified via two previously published methods: responsiveness to varying ecological conditions and slopes of reaction norms. The spikelets/spike was the most plastic trait, with an overall plasticity of 1.62. The least plastic trait was grain protein content, with an overall plasticity of 0.79. The trait hierarchy based on phenotypic plasticity was spikelets/spike > thousand kernel weight > seed number > seed length > grain yield > grain protein content. An increase in yield plasticity of 0.1 was associated with an increase in maximum yield of 4.45 kg ha−1. The plasticity of seed number and grain protein content were significantly associated with yield plasticity. The maximal yield was positively associated with spikelets/spike and grain yield, whereas it negatively associated with grain protein content. In contrast, the minimal yield was found to be negatively related to the plasticity of spikelets/spike and the plasticity of grain yield, whereas it was not related to grain protein content plasticity. Seed number and seed length exhibited plastic responses at the higher fertilisation state while remaining relatively stable at the lower fertilisation state for the wheat DH population. The finding of the current study will play a key role in wheat improvement under the changing climate. Seed length and seed number should be the breeding target for achieving stable yield in adverse environmental conditions. [ABSTRACT FROM AUTHOR]

Glutaredoxins (GRXs) are small-molecular-weight proteins present in a wide range of organisms, and they play a key role in maintaining the redox homeostasis of cells. Most studies on GRXs are conducted in animals and humans, and those conducted on plants are scarce. The number and types of GRX genes vary in different plants. According to the active sites, the GRX family can be further divided into the CPYC, CGFS, and CC subfamilies. The CPYC and CGFS subfamilies are present in eukaryotes. The CC subfamily is exclusively present in higher plants and has the highest number of genes. In this study, 85 GRX genes were identified in common wheat (Triticum aestivum L.) using a bioinformatic method, wherein 12, 9, and 64 belonged to the CPYC, CGFS, and CC subfamilies, respectively. All TaGRX genes were homogeneously distributed in the three subgenomes of wheat. The gene structure analysis revealed that TaGRX members had 1–7 introns. The conserved motif analysis revealed that members of the same TaGRX subfamily had similar motifs. An analysis of cis-regulatory elements of promoters demonstrated that most TaGRX members had auxin-responsive elements; cis-regulatory elements, such as methyl jasmonate (MeJA), MYB, and abscisic acid (ABA), were distributed in all subfamilies, and the cell-cycle regulation element was only observed in the CC and CPYC subfamily members. Additionally, the synteny of the GRX genes in wheat, in wheat and Arabidopsis, and in wheat and barley was analyzed to clarify the evolutionary correlation of TaGRXs. The expression characteristics of TaGRXs were investigated, and TaGRX expression in various tissues and its responses to different abiotic stresses were preliminarily determined. This study provides a reference for the functional analysis of TaGRXs and understanding their role in molecular breeding of wheat. [ABSTRACT FROM AUTHOR]