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The document "Microalgae Biotechnology: Methods and Applications" discusses the potential of microalgae as sustainable chassis for biotechnology, emphasizing their ability to produce valuable compounds and remediate pollutants. Advances in genetic tools and techniques, such as CRISPR/Cas9, are crucial for enhancing microalgal biotechnology. The document also highlights the use of synthetic biology to produce antimicrobial peptides in microalgae, offering a sustainable solution to combat antibiotic resistance. Additionally, innovations in cost-effective microalgae cultivation techniques, such as optimized culture mediums and photobioreactors, are essential for making microalgal products economically viable. [Extracted from the article]

Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failures in sows and respiratory diseases in pigs of all ages. PRRS virus (PRRSV) is its causative agent and has caused huge economic losses in the swine industry. Porcine sialoadhesin (pSn) is a putative receptor of PRRSV. Previous studies have shown that a pSn V-set Ig-like domain is significant in PRRSV infection. However, its structural details are not fully known, hindering our deep understanding of PRRSV infection. In this study, we successfully cloned, expressed and purified the pSn V-set Ig-like domain in Drosophila S2 cells. Then we tried to crystallize the target protein and predicted its structure. This will establish the foundation for the further structural study of pSn, deepen our understanding of the invasion mechanism of PRRSV, and support the structural information for the development of clinical drugs and vaccines against PRRSV.

Two female-specific AFLP (amplified fragment length polymorphism) markers (named CseF464 and CseF136) were isolated by using one selective primer combination (E-AGC/M-CTG) from the genomic DNA of 20 females and 20 males of the half-smooth tongue sole Cynoglossus semilaevis. Both the markers were re-amplified, recovered from the agarose gels, cloned and sequenced. Bioinformatics analysis indicated that the length of the two markers were 468bp and 134bp, respectively, and the sequences showed no similarity to each other, as well as to the known sequences deposited in the GenBank database using BLASTn. Two pairs of SCAR (sequence characterized amplified regions) primers were designed based on the sequences of the two female-specific markers. Furthermore, PCR-based genetic sex identification method was developed in Cynoglossus semilaevis. A specific fragment was amplified in all females but not in any males by using these SCAR primers on the initial 20 female and 20 male individuals of Cynoglossus semilaevis. The feasibility of the two SCAR primer pairs was confirmed in additional 100 individuals (50 females and 50 males). This allowed for reliable, rapid molecular identification of genetic sex of the species, genetic mapping on the sex chromosomes and better understanding of the sex determination and sex differentiation in the half-smooth tongue sole [Current Zoology 55 (4): 309–314, 2009].

In this paper, we first establish the complete convergence for weighted sums of widely orthant-dependent (WOD, in short) random variables by using the Rosenthal type maximal inequality. Based on the complete convergence, we further study the complete moment convergence for weighted sums of arrays of rowwise WOD random variables which is stochastically dominated by a random variableX. The results obtained in the paper generalize the corresponding ones for some dependent random variables. [ABSTRACT FROM AUTHOR]