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18. Comparison of Lycium barbarum fruits polysaccharide from different regions of China by acidic hydrolysate fingerprinting‐based HILIC‐ELSD‐ESI‐TOF‐MS combined with chemometrics analysis.

Author

Zhao, Hengqiang, Wang, Ling, Yu, Yi, Yang, Jian, Zhang, Xiaobo, Zhao, Zhiguo, Ma, Fangli, Hu, Minghua, and Wang, Xiao

Abstract

Introduction: Lycium barbarum is an edible fruit widely used in herbal medicines and as a functional food. Polysaccharide is one of the most important active ingredients. Only L. barbarum grown in the Ningxia region of China are officially recognised as suitable for use in traditional Chinese medicine, but the systematic comparison of L. barbarum polysaccharide between Ningxia and the other growing regions of China has been rarely reported. Objective: To compare the difference of L. barbarum polysaccharide from different grown regions of China. Methods: A chemical fingerprint of L. barbarum polysaccharide hydrolysates was established based on controlled acidolysis combined with hydrophilic interaction liquid chromatography‐evaporative light scattering detection‐electrospray ionisation‐time‐of‐flight‐mass spectrometry (HILIC‐ELSD‐ESI‐TOF‐MS). Then, it was employed for the comparison of L. barbarum samples from different geographical origins of China combined with chemometrics analysis. Results: Six monosaccharides [rhamnose (Rha), xylose (Xyl), arabinose (Ara), mannose (Man), glucose (Glu), galactose (Gal)] were qualitatively and quantitatively determined and four glycoconjugates were preliminarily identified from the hydrolysates. Content determination for the polysaccharide and monosaccharide indicated obvious geographical features. The HILIC‐ELSD fingerprint combined with partial least squares‐discriminant analysis (PLS‐DA) was able to differentiate L. barbarum samples from Ningxia, Xinjiang, Gansu and Qinghai regions with 89.19% classification accuracy. Orthogonal projection to latent structure discriminant analysis (OPLS‐DA) was able to differentiate between samples from Ningxia and those from the other three growing regions, polysaccharide and Ara were the potential chemical markers. Conclusions: These findings form the basis of a reliable method to trace the region of origin of L. barbarum sample and thereby, improve the quality control of L. barbarum therapeutic polysaccharides. A chemical fingerprint of L. barbarum polysaccharide hydrolysates was established based on controlled acidolysis and HILIC‐ELSD‐ESI‐TOF/MS. Then, it was employed for the comparison of L. barbarum samples from different geographical origins of China combined with chemometrics analysis. PLS‐DA was able to differentiate L. barbarum samples from Ningxia, Xinjiang, Gansu and Qinghai regions with 89.19% classification accuracy. OPLS‐DA classified samples between Ningxia and other regions, polysaccharide and Ara were the potential chemical markers. [ABSTRACT FROM AUTHOR]

Published
2023
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22. Rapid Quantification of Bioactive Lentinan with an Aniline Blue Fluorescent Method

Author

Ma Fangli, Tong Li, Jiangwei Tian, Hu Minghua, Fan Luodi, and Boyang Yu

Subjects
Detection limit, Chromatography, 010405 organic chemistry, Lentinan, ALIZARIN RED, Buffer solution, 01 natural sciences, Fluorescence, 0104 chemical sciences, Immune modulator, 03 medical and health sciences, Fluorescence intensity, chemistry.chemical_compound, 0302 clinical medicine, chemistry, 030220 oncology & carcinogenesis, Selectivity
Abstract

Lentinan is a clinically approved immune modulator and its anticancer and immunomodulatory bioactivity is found to be dependent on its triple helical conformation. Therefore, the development of rapid and convenient method for determination of bioactive lentinan with triple helical conformation holds great promise for the quality control of lentinan healthy products. In this work, an aniline blue fluorescent method was optimized and established to accurately and rapidly detect bioactive lentinan. In the presence of lentinan, the fluorescence intensity of aniline blue with 404 nm excitation and 492 nm emission dramatically enhanced within 15 min in pH 10 glycine-NaOH buffer solution, which allowed the analysis of lentinan in a very simple and fast manner. The method allowed for the sensitive determination of lentinan in the range of 1 to 60 μg/mL with a detection limit of 0.25 μg/mL. Notably, the protocol exhibited excellent selectivity for the determination of triple helical lentinan over other saccharides. The method was successfully applied to the detection of bioactive lentinan in health tonic solution, which demonstrated the method had great potential for quality control of lentinan contained products.

Published
2019

24. Cinnamaldehyde Treatment of Prostate Cancer-Associated Fibroblasts Prevents Their Inhibitory Effect on T Cells Through Toll-Like Receptor 4

Author

Mei,Jie, Ma,Jing, Xu,Yuwei, Wang,Yuanyuan, Hu,Minghua, Ma,Fangli, Qin,Zhihai, Xue,Rui, and Tao,Ning

Subjects
Drug Design, Development and Therapy
Abstract

Jie Mei,1– 3,* Jing Ma,2,4,5,* Yuwei Xu,4 Yuanyuan Wang,6 Minghua Hu,6 Fangli Ma,6 Zhihai Qin,1,2,4 Rui Xue,1 Ning Tao2 1Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People’s Republic of China; 2Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing, People’s Republic of China; 3College of Life Science & Technology, Huazhong University of Science and Technology, Wuhan, People’s Republic of China; 4College of Life Science, University of the Chinese Academy of Sciences, Beijing, People’s Republic of China; 5School of Basic Medical Sciences of Southwest Medical University, Luzhou, People’s Republic of China; 6Infinitus Chinese Herbal Immunity Research Center, Infinitus China Company Ltd, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhihai Qin; Ning TaoKey Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, No. 15 Datun Road, Chaoyang Area, Beijing City, People’s Republic of ChinaEmail zhihai@ibp.ac.cn tao@ibp.ac.cnIntroduction: Cancer-associated fibroblasts (CAFs) promote tumor progression; thus, drugs that can modify CAFs need to be identified.Methods: To test the effect of cinnamaldehyde on prostate CAFs, the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-2H-tetrazolium bromide assay was used to determine their survival. When spleen cells were treated with CAF supernatant, the proliferation of T cells was inhibited as determined by flow cytometry. After cinnamaldehyde treatment, this immunosuppressive function of CAFs was partially reversed. To explore the molecular mechanism, Western blotting and the quantitative real-time polymerase chain reaction were applied, and TLR4-dependent signaling pathway-related protein and mRNA levels were quantified.Results: Cinnamaldehyde acted on the TLR4-dependent signaling pathway, altering the function of CAFs such that its supernatant no longer inhibited the proliferation of T cells.Conclusion: These data indicate that cinnamaldehyde can modify the functions of CAFs, which may be helpful for treating tumors. Cinnamaldehyde can suppress CAF T-cell inhibition.Keywords: cinnamaldehyde, cancer-associated fibroblasts, prostate cancer, TLR4

Published
2020

25. Spectrum‐effect relationship between fingerprints and hemopoietic effects of small molecular fraction of Polygoni Multiflori radix praeparata

Author

Ma Fangli, JunXing Wang, Qi Jin, Hu Minghua, and Fan Luodi

Subjects
Male, Erythrocytes, Clinical Biochemistry, Cell Count, Hematocrit, Pharmacology, 030226 pharmacology & pharmacy, 01 natural sciences, Biochemistry, Analytical Chemistry, Hemoglobins, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Drug Discovery, medicine, Animals, Radix, Aplastic anemia, Molecular Biology, Chromatography, High Pressure Liquid, Mice, Inbred ICR, Hematologic Tests, Chromatography, medicine.diagnostic_test, 010401 analytical chemistry, Anemia, Aplastic, General Medicine, medicine.disease, 0104 chemical sciences, Disease Models, Animal, Red blood cell, Haematopoiesis, medicine.anatomical_structure, chemistry, Hematinics, Gray relational analysis, Hemoglobin, Emodin, Drugs, Chinese Herbal
Abstract

Polygoni multiflori Radix Praeparata (PMRP) is a traditional medicine used for nourishing essence and blood in China. However, it is unclear which PMRP compounds are responsible for its hematopoietic effect. In this study, spectrum-effect relationship was used to discovery potential hematopoietic compounds. The fingerprints of 20 PMRP batches were established by HPLC and the hematopoietic effect was determined using red blood cell, hemoglobin, hematocrit, and platelet indexes in aplastic anemia model mice. The spectrum-effect relationship between common peaks and hematopoietic efficacy values was established using gray relational analysis and partial least squares analysis. Spectrum-effect relationship results showed that peaks 21 (emodin-8-O-(6´-O-acetyl)-β-D-glucoside), 15 (2, 3, 5, 4'-tetrahydroxystilbene-2-O-di-glucoside), 16 (cis-2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glucoside), 11 (unknown), 20(unknown, 12 (epicatechin), 29 (carboxyl emodin), and 31 (emodin) in the fingerprints were closely related to the hematopoietic effect. This work successfully established the spectrum-effect relationship between PMRP hematopoietic effect and its fingerprints, which can be used to explain the material basis for the PMRP hematopoietic effect.

Published
2020

29. Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities

Author

Zou, Zihua, primary, Wei, Ming, additional, Fang, Jie, additional, Dai, Wei, additional, Sun, Tingting, additional, Liu, Qian, additional, Gong, Guiping, additional, Liu, Yuxia, additional, Song, Shuang, additional, Ma, Fangli, additional, Wang, Langhong, additional, Huang, Linjuan, additional, and Wang, Zhongfu, additional

Published
2020
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