1. Detecting MYB and MYBL1 Fusion Genes in Tracheobronchial Adenoid Cystic Carcinoma by Targeted RNA-Sequencing
- Author
-
Jianming Pei, Arthur S. Patchefsky, Harry S. Cooper, Douglas B. Flieder, Jacqueline Talarchek, Joseph R. Testa, and Shuanzeng Wei
- Subjects
0301 basic medicine ,Male ,Pathology ,medicine.medical_specialty ,Oncogene Proteins, Fusion ,Adenoid cystic carcinoma ,MYB-NFIB ,Chromosomal translocation ,Tracheobronchial Adenoid Cystic Carcinoma ,Biology ,Adenoid ,Article ,Pathology and Forensic Medicine ,lung ,Fusion gene ,03 medical and health sciences ,Proto-Oncogene Proteins c-myb ,0302 clinical medicine ,Proto-Oncogene Proteins ,medicine ,Carcinoma ,Humans ,MYB ,MYBL1-RAD51B ,Aged ,Aged, 80 and over ,Lung ,Salivary gland ,Bronchial Neoplasms ,High-Throughput Nucleotide Sequencing ,RNA sequencing ,Middle Aged ,medicine.disease ,MYBL1-NFIB ,Carcinoma, Adenoid Cystic ,3. Good health ,stomatognathic diseases ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Trans-Activators ,Female ,Tracheal Neoplasms - Abstract
Primary tracheobronchial adenoid cystic carcinoma is rare, accounting for less than 1% of all lung tumors. Many adenoid cystic carcinomas have been reported to have a specific chromosome translocation t(6;9)/MYB-NFIB. More recently, t(8;9)/MYBL1-NFIB gene fusion was reported in salivary gland adenoid cystic carcinomas which lacked a t(6;9)/MYB-NFIB. Two prior studies showed t(6;9)/MYB-NFIB in tracheobronchial adenoid cystic carcinoma; however, only rare cases of MYBL1 rearrangement have been reported in this carcinoma. In this study, we used targeted RNA sequencing to investigate fusion genes in tracheobronchial adenoid cystic carcinoma at our institution. Fusions of either MYB or MYBL1 genes were detected in 7 of 7 carcinomas. Three cases had MYB-NFIB, and 3 had MYBL1-NFIB. The remaining case showed a rare MYBL1-RAD51B fusion. These findings suggest that rearrangement involving MYB or MYBL1 is a hallmark of tracheobronchial adenoid cystic carcinoma.
- Published
- 2019