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ABSTRACT Many bacteria possess proteasomes and a tagging system that is functionally analogous to the ubiquitin system. In this system, Pup, the tagging protein, marks protein targets for proteasomal degradation. Despite the analogy to the ubiquitin system, where the ubiquitin tag is recycled, it remained unclear whether Pup is similarly recycled, given how the bacterial proteasome does not include a depupylase. We previously showed in vitro that as Pup lacks effective proteasome degradation sites, it is released from the proteasome following target degradation, remaining conjugated to a degradation fragment that can be later depupylated. Here, we tested this model in Mycobacterium smegmatis, using a Pup mutant that is effectively degraded by the proteasome. Our findings indicate that Pup recycling not only occurs in vivo but is also essential to maintain normal pupylome levels and to support bacterial survival under starvation conditions. Accordingly, Pup recycling is an essential process in the mycobacterial Pup‐proteasome system. [ABSTRACT FROM AUTHOR]

In practical engineering applications, factors like dust adhesion and environmental changes can cause photovoltaic arrays to exhibit multiple peaks in output power. An optimization algorithm with global optimization capability is needed to track its maximum power. In this regard, this paper proposes an improved marine predator algorithm (IMPA) to extract the maximum power point of photovoltaic system under complex solar irradiation conditions. To overcome the issues in the traditional marine predator algorithm (MPA), the opposition-based learning(OBL) strategy is introduced in IMPA, and the sine cosine algorithm (SCA) is integrated into the iteration stage to enhance the search ability of the algorithm. Furthermore, the low-order converter in the traditional MPPT control system is replaced by the Zeta converter, which increases the operating voltage range. Ultimately, simulation results demonstrate that the MPPT based on IMPA has higher tracking efficiency and shorter response time.The experimental results also indicate the practical feasibility of this method, as well as its high level of stability and robustness. [ABSTRACT FROM AUTHOR]

Marine protected areas (MPAs) are widely promoted as effective tools for conserving biodiversity and safeguarding ecosystem services. However, MPA success can be hindered by a lack of legitimacy and low social support because of perceived negative effects on ecosystem services and human well-being. Despite these social challenges, the social dimensions of marine conservation, such as the effects of MPAs on coastal livelihoods and local communities' perceptions of these effects, are often overlooked in conservation initiatives. In this study, we use a mixed methods approach, combining qualitative thematic analysis and quantitative network analysis derived from interviews and focus group discussions, to examine the perceptions of key stakeholder groups about the Litoral Norte MPA in Portugal. Our findings reveal that most stakeholder groups hold more negative than positive views about the governance and management of the MPA. Key concerns include unsatisfactory participation in MPA decisions and perceptions that the MPA fails to deliver positive social and ecological outcomes, such as increased community involvement, fair income distribution, and enhanced fish abundance. Policy makers and managers need to address these negative perceptions to improve conservation governance and management. By considering the stakeholder feedback presented in this study, such as fostering better engagement with the local community and transforming conflicts into opportunities for co-developing new conservation actions with local resource users, policy makers and managers can increase support for Litoral Norte and enhance the social and ecological outcomes of the MPA. [ABSTRACT FROM AUTHOR]

Effective seabird conservation requires understanding their marine spatial ecology. Tracking can reveal details of their foraging ecology and habitat use, as well as the suitability of marine protected areas for at-sea conservation, but results are often regionally specific. Here we characterised the foraging behaviour of tropical breeding brown boobies Sula leucogaster in the Chagos Archipelago, Western Indian Ocean, and tested habitat requirements. GPS tracking of thirteen individuals from two colonies, located 142 km apart on the same atoll (Great Chagos Bank), showed similar foraging effort and habitat preferences despite differences in season and breeding stage. Brown boobies from both tracked populations foraged close to the colony along the atoll shelf edge, avoiding deep oceanic areas and shallow waters of the Great Chagos Bank atoll, but within the Chagos Archipelago Marine Protected Area. Sea-level height anomaly and sea surface temperature were important foraging predictors at both sites, although birds experienced distinct environmental conditions between colonies. These results suggest that while brown boobies have colony-specific at-sea foraging areas, similarities in habitat drivers of distribution and foraging behaviour can inform predictions of distributions at other colonies within the archipelago, with important benefits for at-sea conservation efforts. [ABSTRACT FROM AUTHOR]

Networks of marine protected areas (MPAs) are being increasingly implemented worldwide as conservation management tools. We report here on MPA effectiveness using data from a 17‐year long remotely operated vehicle (ROV) monitoring program spanning mesophotic (~ 20–130 m) depths in 26 MPAs across California's MPA network. We utilize a spatial modeling approach that includes important environmental covariates as well as spatial dependence in the data, and allows the separation of statewide and regional trends in the abundance of focal species from additional trends specific to MPAs. We demonstrate that there have been statewide and/or regional recoveries in abundance for the majority of our 10 focal demersal fish species, with all four statewide species assessments and 18 out of 22 species‐region combinations assessed displaying positive trends. We also demonstrate that MPA protection has had an additional positive effect on the abundance of the majority of these focal species inside MPAs compared with reference areas, with positive effects for all four statewide species, and 18 out of 22 statewide/regions assessed showing positive effects, four showing no statistically detectable differences, and no negative MPA effects found. Comparisons with theoretical expectations of MPA recovery for our focal species showed that 2 out of 4 statewide, and 11 out of 17 species‐region combinations assessed displayed higher mean MPA effects than expected. Our results highlight strong trajectories of increasing abundance and additional MPA effects for many of our focal species, demonstrating that MPAs are having positive effects in mesophotic depths across the network as well as at previously reported shallower depths, and that image‐based platforms such as ROVs provide an important tool to support timely reporting on the effectiveness of MPA networks. [ABSTRACT FROM AUTHOR]

Coral reef ecosystems are facing severe degradation due to anthropogenic activities at both local and global scales. In response, extensive restoration efforts are underway, aiming to bolster coral cover and enhance reef fish communities to foster facilitation between fish and corals. This reciprocal relationship is anticipated to improve overall restoration efficacy and enhance coral reef resilience in the face of global warming. Here, we investigate the impact of coral restoration using out-planted Acropora cervicornis colonies attached to raised domes on the associated fish community on the isolated, well-protected reef of Little Cayman Island in the Central Caribbean. Surveys were conducted immediately preceding out-planting, five days later, and 85 days later to capture temporal changes in the fish community. After 85 days of out-planting, there were no changes in fish biomass, abundance, or species richness for the entire fish community. This pattern was consistent for selected fish functional groups. Additionally, no significant differences were observed in the fish community before outplanting, five days after out-planting, or 85 days after out-planting of restoration domes. Our results underscore the limited impact of coral restoration for influencing fish communities in the isolated and highly protected reef of Little Cayman over an 85-day period. Consequently, our findings have implications for using coral restoration as a mechanism to enhance fish populations, particularly in marginally disturbed regions where structural complexity has not been lost. Future restoration programs should therefore incorporate local knowledge of environmental history and restoration needs along with an increased data-driven understanding of the intricate interaction between fish and coral populations to be successful. [ABSTRACT FROM AUTHOR]

Objective To evaluate the ACR/EULAR 2022 criteria for ANCA-associated vasculitides (AAV) classification and compare them with the European Medicines Agency (EMA) algorithm and with classification based only on ANCA serology. Methods In the analysis, 374 cases (47% female) were classified according to the EMA algorithm, ANCA serology and ACR/EULAR criteria. The agreement rate was calculated using the kappa (κ) statistic. Results Under EMA, 192 patients were classified as granulomatosis with polyangiitis (GPA), 159 as microscopic polyangiitis (MPA) and 23 as eosinophilic granulomatosis with polyangiitis (EGPA). The ACR/EULAR criteria classified 199 patients as GPA, 136 as MPA and 22 as EGPA. Four patients (1.1%) met criteria of two disease categories, and 13 (3.5%) were unclassifiable. The observed agreement between EMA and ACR/EULAR was 85% for GPA, 75% for MPA and 96% for EGPA. The unweighted κ statistic was 0.66 (95% CI: 0.60, 0.74). Of the 188 PR3-ANCA positive patients, 186 (98.9%) were classified as GPA using ACR/EULAR criteria, and 135 of 161 (83.9%) MPO-ANCA positive patients were classified as MPA. With a classification solely based on ANCA specificity, agreement with ACR/EULAR was 99% for GPA and 88% for MPA. Conclusions EMA and ACR/EULAR classification give similar results. A small proportion of patients cannot be classified or fall into two categories. Some patients exhibiting granuloma, a key feature of GPA, are nevertheless classified as MPA, conflicting with the current view of histopathology of AAV. There is high agreement of ANCA-based classification with that of ACR/EULAR, reflected in the considerable weight granted to ANCA in the new criteria. These crucial elements within the new criteria necessitate a consensus discussion among field experts. [ABSTRACT FROM AUTHOR]

Micro-pyramid copper molds are critical components in the preparation of high-precision optical elements, such as light-trapping films and reflective films. Their surfaces are composed of micro-pyramid arrays (MPAs). The surface roughness and edge burrs of MPAs seriously affect the optical properties of optical elements. To reduce the surface roughness, as well as the sizes of the edge burrs, the longitudinal ultrasonic vibration-assisted planing (LUVP) method for processing MPAs was developed during this study. In addition, an experiment was conducted to compare the precision planing and LUVP methods of MPA generation. The results show that the tool nose amplitude of the LUVP experimental platform constructed during this study was 3.3 μm, and that the operating frequency was 19.85 kHz. An MPA processed by LUVP had a smaller surface roughness than that of an MPA produced by precision planing; it also had fewer and smaller edge burrs, and there was slightly less diamond tool wear. The MPA cut using the LUVP method had no corrugation on its surface. This research lays a foundation for developing higher-precision micro-pyramid plastic films. [ABSTRACT FROM AUTHOR]

Abstract In practical engineering applications, factors like dust adhesion and environmental changes can cause photovoltaic arrays to exhibit multiple peaks in output power. An optimization algorithm with global optimization capability is needed to track its maximum power. In this regard, this paper proposes an improved marine predator algorithm (IMPA) to extract the maximum power point of photovoltaic system under complex solar irradiation conditions. To overcome the issues in the traditional marine predator algorithm (MPA), the opposition-based learning(OBL) strategy is introduced in IMPA, and the sine cosine algorithm (SCA) is integrated into the iteration stage to enhance the search ability of the algorithm. Furthermore, the low-order converter in the traditional MPPT control system is replaced by the Zeta converter, which increases the operating voltage range. Ultimately, simulation results demonstrate that the MPPT based on IMPA has higher tracking efficiency and shorter response time.The experimental results also indicate the practical feasibility of this method, as well as its high level of stability and robustness.

Music performance anxiety (MPA) is recognized as a distinct emotional behavior rather than merely a motor control disorder and is influenced by specific conditioning experiences. This study investigates the interrelationships between MPA, self-efficacy, and future career expectations among music students within the Chinese context. The participants of this study were 340 high school students majoring in music education and performance, drawn from three music schools in China. Data were collected using several questionnaires: the MPA Inventory for Adolescents (MPAI-A), the Self-Efficacy Formative Questionnaire, and the Career Futures Inventory (CFI). The findings indicate that MPA is negatively associated with self-efficacy and future career expectations. Additionally, self-efficacy acts as a partial moderator between MPA and career expectations, suggesting that enhancing the self-efficacy of music students can boost their future career aspirations and mitigate the adverse effects of MPA. This research explores the complex relationships among MPA, self-efficacy, and future career expectations, emphasizing the importance of curriculum and pedagogical strategies in music schools. Music students with high levels of self-efficacy may exhibit more confidence and stable performances before audiences. According to the panel regression analysis, self-efficacy significantly positively influences career expectations. An appropriate educational environment and supportive pedagogical approaches to MPA can foster the early career development of musicians. [ABSTRACT FROM AUTHOR]

The harbour porpoise (Phocoena phocoena) is considered part of the ‘Outstanding Universal Value’ characterising the Wadden Sea World Heritage Site (WS WHS). The Trilateral Wadden Sea Plan aims to preserve the conservation status of the Trilateral Wadden Sea Cooperation Area, encompassing the WS WHS. The plan has specified two conservation targets for the harbour porpoise: (1) viable stocks and a natural reproduction capacity and (2) conservation of habitat quality for its conservation. To assess the current occurrence of the harbour porpoise in the Wadden Sea area, we collated and analysed data from regional and national research projects using telemetry, aerial surveys, strandings and passive acoustic monitoring, obtained over the years 1990–2020. The results illustrate that porpoises occur in both offshore and intertidal waters, showing seasonal movements and changes in local occurrence over time. Some porpoises displayed limited home ranges throughout the year, suggesting a possible residency for some of the animals using the Wadden Sea area. We also showed that methods, frequency and spatial coverage of monitoring activities vary among the countries Denmark, Germany and the Netherlands. We discuss the suitability of the different methods both regarding the challenges of monitoring in the complex Wadden Sea habitat as well as their ability to target the conservation aims of the WHS. We give several recommendations to assess the status of the species to meet the identified conservation aims. [ABSTRACT FROM AUTHOR]

Misty Groupers (Hyporthodus mystacinus) are one of the largest and most geographically widespread grouper species and one of the few grouper species known to occur at depths greater than 200 m. However, aspects of their basic biology, behavior, and ecology remain poorly understood, leaving significant gaps in our ability to evaluate their functional role throughout the vertical water column, as well as our understanding of their conservation needs in a changing ocean. Through in-situ video observation obtained using deep-sea landers in both The Bahamas and Cayman Islands over multiple years, we documented Misty Grouper occurrence up to 470 m depth in the mesopelagic zone. These observations provide a new depth range extension for the species and illuminate the potential importance of deep-water habitats for large grouper species in the wider Caribbean. [ABSTRACT FROM AUTHOR]

Overgrazing may lead to management intervention (e.g. culling, animal relocation) to try and prevent habitat destruction. Overgrazing leading to seagrass meadow collapse has been recorded for green turtles (Chelonia mydas) at several sites around the world, although the generality of this phenomenon and the need for intervention to prevent widespread seagrass destruction is unknown.Where turtles have degraded seagrass meadows, home‐ranges are expected to be large and turtles will relocate as meadows are destroyed. We used high resolution Fastloc GPS tracking (n = 32 individuals, mean = 171 days per individual, SD = 99) to record the home‐range of adult green turtles at foraging sites spanning 4523 km of longitude across the Western Indian Ocean. Contrary to predictions if overgrazing was occurring, we recorded small home‐ranges and turtles rarely relocated their daytime foraging areas.Based on all locations received, the mean 50% and 95% utilisation distributions (UD50 and UD95) were 2.4 km2 (SD = 2.7) and 15.4 km2 (SD = 17.7). Space use was often particularly small at night, when turtles rest, averaging 11% of the overall space use with the mean night‐time UD50 and UD95 being 0.15 km2 (SD = 0.1) and 1.1 km2 (SD = 0.8), respectively. Variation in home‐range across individuals was not influenced by the data volume (number of locations per day, duration of tracking) or animal size (carapace length) but increased significantly as the distance between the centre of day and night areas increased, that is individuals that had a larger daily commute had the larger home‐ranges.Synthesis and applications. Comparisons with home‐range estimates from 16 previous studies, showed that those we recorded are among the smallest for adult green turtles globally. These results suggest that despite population size increases at several major nesting sites in the Western Indian Ocean, green turtles are generally not destroying the seagrass meadows on which they forage and so management intervention to prevent overgrazing is not needed. In this way, our work illustrates how movement data may inform management decisions for green turtles. Further targeted work on the seagrass ecosystem health could help confirm this suggestion. [ABSTRACT FROM AUTHOR]

This study aimed to determine the effectiveness of a self-help application to reduce performance-related excitement in students of solo vocals in higher education institutions. The study participants (n = 219) used the mobile application during 6 weeks. Statistically significant effect of the intervention was achieved by Negative cognitions, Psychological vulnerability, and Anxiety perception constructs. The study also examines the influence of sociodemographic and personal characteristics on anxiety. Gender, graduate status, and self-efficacy were statistically significant variables when using the psychological self-help application. The investigation failed to disclose any significant impact of performance experience. Psychological self-help applications can be used in vocal/music education as a low-threshold intervention to reduce anxiety symptoms. The findings of the study introduce new data into approaches to the treatment of anxiety and expand the understanding of the characteristic features of singer training.

Marine fish communities suffer from anthropogenic pressures and climate change, which influence their spatio-temporal dynamics. Marine Protected Areas (MPAs) have been established worldwide to preserve these communities, while mesophotic ecosystems could provide natural refugia. Assessing the extent to which MPAs and deeper ecosystems can mitigate human and climate change impacts requires regular monitoring of temporal community dynamics. Environmental DNA (eDNA) surveys – being time- and cost-effective – can provide valuable insights on biodiversity change. Here, we initiated a long-term study based on eDNA monitoring in an MPA in the north-western Mediterranean Sea that includes areas with various protection levels. Specifically, from June 2021 to January 2023, we collected eDNA samples during the summer, fall, and winter seasons from shallow water (20 m depth), at 40 m depth, and from the mesophotic zone (80 m depth) in a Fully Protected Area (FPA) and in a nearby Lightly Protected Area (LPA) in the Riou archipelago (France). In this short period and relatively small area, we detected a total of 113 actinopterygian and chondrichthyan taxa. Species with high fishing vulnerability had higher detection rates in the FPA than in the LPA, suggesting a positive impact of FPAs on the conservation of these threatened species. A marked seasonal signal in species detections, including significantly lower detections of several species in winter, indicated a combined effect of species biological changes and migration behavior. The seasonality trend was stronger in the FPA than in the LPA, indicating that such areas may modify sub-yearly patterns in communities and ecosystem processes. Fish composition was associated with water depth, with marked species dissimilarities between shallow waters and the mesophotic zone, implying that multiple depths should be considered in MPA monitoring to fully capture the response of biodiversity to management. Our results point to the importance of temporal information combined with extensive sampling across depths and protection levels to fully understand the ecological dynamics and structure of coastal fish communities.

Artificial reefs have been suggested as alternative dive sites to mitigate human pressure on natural reefs. Despite the conceptual appeal of artificial reefs, there is a paucity of empirical evidence regarding their effectiveness in achieving this objective. Here, we report that a small artificial reef deployed adjacent to a local coral marine protected area caused a shift in the routes taken by introductory dives and nearly eliminated their visitations to the natural fringing reef within the MPA. This behavioral shift among divers persisted for more than a decade following the AR deployment. These findings underscore the efficacy of well-designed and appropriately located artificial reefs as valuable instruments in the conservation of coral reefs.

A novel acquisition scheme of pseudo-noise (PN) codes is proposed for spreading satellite communication systems relying on the proposed multi-voter model of this paper. Based on the proposed model, the acquisition of PN codes can be attributed to a voting and selecting mechanism to pick out the erroneous chips. Although message passing algorithm (MPA) is a feasible algorithm for decoding PN codes, MPA does not get good detection performance due to the limited number of parity check equations in the acquisition scheme. To overcome the negative impact of the limited number of parity check equations, this paper proposes single-voter and multi-voter models, which combine chip and sequence estimation on the basis of the chip-flipping (CF). It is known that the bit-flipping (BF), weighted-bit-flipping (WBF) and other algorithms based on BF are credible for low density parity check (LDPC) codes. Because of the lack of research, these algorithms have not been extended to the detection of PN codes. As the same as BF, the inputs of the proposed CF algorithm are the hard-decision samples. For the proposed CF, there exists an optimal flipping-threshold which is similar to the random weight of the WBF. Owing to the low computation complexity of CF, the unlimited number of parity check equations can be enabled in the voting model. The experimental results show that the detection performance of the proposed method of $N > $ 15 is improved by 2 dB compared with MAP-based method and 4 dB compared with LEAP-based method at the detection probability 99%.

Atlantic cod (Gadus morhua Linnaeus, 1758) can experience mortality at sub-zero (<0 °C) temperatures in ice-infested waters, and many populations migrate to deeper and warmer areas during winter. An exception is the resident population in Gilbert Bay, Labrador, which annually experiences 6 months of sub-zero temperatures. We used the VEMCO Positioning System to estimate fish locations to determine depth utilization and seasonal movements of tagged Gilbert Bay cod ranging in size from 43 to 75 cm. This is the first study to directly monitor the movements of adult Atlantic cod for extended periods (months) in sub-zero temperatures. Gilbert Bay cod remained active under an ice-covered surface during the 6 months of sub-zero temperatures. During both warm (>0 °C) and cold (≤0 °C) periods, fish spent similar amounts of time not moving, moving slowly, and moving at medium and fast rates. They tended to utilize shallow depths, <10 m, beneath surface ice during winter, while utilizing deeper depths as well during other times of the year. Surprisingly, fish that resided within our tracking array year-round had greater cumulative swimming distances during sub-zero periods compared to warmer periods. No tracked fish died during winter, further highlighting the cold adaptiveness of this population and its importance to biodiversity conservation. [ABSTRACT FROM AUTHOR]

Artificial reefs have been suggested as alternative dive sites to mitigate human pressure on natural reefs. Despite the conceptual appeal of artificial reefs, there is a paucity of empirical evidence regarding their effectiveness in achieving this objective. Here, we report that a small artificial reef deployed adjacent to a local coral marine protected area caused a shift in the routes taken by introductory dives and nearly eliminated their visitations to the natural fringing reef within the MPA. This behavioral shift among divers persisted for more than a decade following the AR deployment. These findings underscore the efficacy of well-designed and appropriately located artificial reefs as valuable instruments in the conservation of coral reefs. [ABSTRACT FROM AUTHOR]

Predicting and measuring changes resulting from marine protected areas (MPAs) has posed a challenge for practitioners, partly because ecosystems are complex and can change in unanticipated ways, but also due to MPA characteristics such as design factors, conservation objectives (COs), and monitoring programs, that can leave little chance of meeting stated goals. We consider these design factors for the Laurentian Channel MPA, a large offshore Canadian protected area established to protect against fishing impacts. Specifically, in this study we evaluated (1) whether it is realistic to expect improvements in the MPA for four previously established taxa‐specific COs, and (2) whether existing scientific surveys are capable of detecting changes in these CO taxa even if they occurred. Three CO species were sampled in scientific multispecies research vessel trawl surveys (Black Dogfish, Smooth Skate, and Northern Wolffish) and a fourth CO, sea pen taxa, were enumerated using seafloor imagery. Simulations indicate that trawl surveys have very little chance of detecting change in the abundance of the three fish species examined, while seafloor imagery data had higher statistical power for sea pen taxa. Moreover, we show that expecting change related to the removal of fishing is unrealistic due to the fact that the MPA was established in an area of minimal fishing pressure. While positive change is unlikely to be induced by the MPA, or be detected if they occurred, this MPA could provide conservation benefits if COs and monitoring approaches were realigned to match the unique features of this area that represents largely unimpacted sensitive benthic habitats. [ABSTRACT FROM AUTHOR]

The topic of this paper is the usage of the algorithm of marine predator (MPA) in the optimization of the suspension of rail vehicles with coil springs. The aim is to reduce the mass of set of coil springs as the main parts of rail vehicles suspension. The optimized set of coil springs must satisfy the appropriate conditions related to the suspension characteristics, with the aim of achieving the required operation quality and running security of the observed rail vehicle. Starting from the bi-linear characteristic of rail vehicles suspension and the analytical description of its parameters, an optimization problem is formulated. It is composed of six optimization parameters, an objective function and 16 constraints (eight for each coil spring in the set). The developed approach is applied in two specific examples of suspension optimization of four-axled freight wagons, the first with axle load of 200 kN and the second with axle load of 225 kN. The optimization problem is resolved using MPA. The acquired results showed that the given optimization approach with MPA provide a significant mass decrease compared to conventional design method of rail vehicles suspension with coil springs. The mass decrease of one set of coil springs in both examples is about 15.5%. Given that each considered four-axled freight wagon has 16 sets of coil springs, the decrease of the total coil springs mass per wagon in both examples is more than 60 kg. Since certified rail vehicles are commonly produced in large series, the proposed approach can be very significant for increasing profitability in the rail vehicles industry. [ABSTRACT FROM AUTHOR]

Marine invaders, including the green crab (Carcinus maenas), cause widespread disruption in their invaded ranges. In a Marine Protected Area in Atlantic Canada, the concurrent collapse of a protected strain of Irish moss (Chondrus crispus) and establishment of green crabs prompted a removal program in 2016–2018. We evaluated the outcome of that program using surveys within and outside (control) the MPA that preceded (2015 and 2016) and ran parallel to the removal program. We used generalized linear models to assess effects on catch rates and demographics and then compared those results to the outcome of other removal programs using the framework of recruitment and growth overfishing. Green crab abundance increased between 2015 and 2017 despite removal efforts, but the increase of both males and females was suppressed compared to control sites, to 29% and 36% of expected numbers, respectively. Between 2017 and 2018, males became significantly smaller relative to control sites. Although green crab eradication is not achievable, benefits would accrue if recruitment overfishing could suppress populations to levels that could be maintained by modest harvesting. Comparing among studies showed that removal did not reduce populations or that supressed populations could rebound quickly. However, within-year population reductions and shifts to smaller sizes, which are consistent with growth overfishing, show that removal programs may achieve short-term/local benefits but seem unable to sustainably suppress populations. At larger scales, the green crab's status as one of the most resilient invaders is likely to remain. [ABSTRACT FROM AUTHOR]

Aiming at the problem of delays of multiple users arriving at the base station in the uplink sparse code multiple access (SCMA) macro-cell system, asynchronous detection algorithms were proposed with users grouped according to different delays and the same information between symbols was utilized and iterated, forming a message passing algorithm based on serial propagation ( SPMPA). Besides, aiming at the problem of worse bit error rate caused by partial marginalization algorithm used in MIMO-SCMA system, dynamic threshold partial marginalization algorithm (DTPM-MPA) was proposed. The simulation results show that the high bit error rate caused by symbol interference of asynchronous transmission is effectively reduced with SP-MPA and a flexible balance between computing complexity and bit error rate is formed with DTPM-MPA. [ABSTRACT FROM AUTHOR]

Groupers are marine fishes particularly vulnerable to overexploitation owing to their reproduction‐related traits. The dusky grouper Epinephelus marginatus is classified as 'Endangered' in the Mediterranean Sea, where protection measures have proven critical for allowing population recovery and persistence of the species. However, knowledge gaps remain regarding its reproductive behaviours and spawning sites. As with other grouper species, the dusky grouper is known to produce courtship‐associated sounds, which were validated in captivity, but no study has ever established a link between sound production and observed behaviours in the wild.This study aims to characterize, both visually and acoustically, the behavioural patterns of wild dusky grouper, thus specifically linking visual courtship behaviours to the associated sound production. Visual as well as passive acoustic methods were employed at two presumptive spawning sites within a Mediterranean Marine Protected Area (MPA) in the north‐western Mediterranean Sea over two consecutive summer seasons (2017 and 2018).The temporal patterns of sound production showed site specificity over the month in which reproductive activity was most intense. Courtship calls were mostly recorded around sunset at both study sites and were significantly associated with visual reproductive behaviours. Results also indicated a temporal partitioning in the acoustic activity of the species, with different sound types being recorded at distinct times of the day, likely corresponding to the occurrence of various behaviours during those periods.These findings have important implications for identifying and monitoring dusky grouper spawning sites using passive acoustic methods, therefore providing valuable information for the development, implementation, and/or enforcement of effective conservation measures therein. [ABSTRACT FROM AUTHOR]

Hormone replacement therapy (HRT), also known as menopausal hormone therapy (MHT), was looked upon as a fountain of youth that kept women young and reduced cardiovascular disease. This led to a large-scale study called the Women's Health Initiative (WHI) that was conducted to show the cardiovascular benefits of HRT. This study was suspended early because of adverse side effects. The USFDA responded by slapping a “black box” warning on all HRT products. USFDA-approved bioidentical HRT formulations are safe and effective. We propose that these formulations have the “black box” warning removed so that doctors feel more confident in prescribing these products for symptoms of menopause and chronic conditions such as cardiovascular health. We propose eliminating the sale of products containing medroxyprogesterone acetate (MPA) because of the increased risk of heart attacks and breast cancers associated with this medication.

This paper presents the design of a microstrip patch antenna based on a Square Split Ring Resonator (SSRR). Wireless technology is switching from 4G to 5G due to the need to overcome limitations, such as low throughput, high latency and path loss. To increase data transfer speeds, the next generation of wireless networks uses 5G terahertz technology. The use of microstrip patch antennas in wireless technologies has increased significantly due to their low cost and simplicity of design as well as the ease of printed circuit board fabrication. However, in some cases their use is limited by low bandwidth, low gain and low throughput. To solve these problems, the Fennec Fox optimization algorithm is used. The algorithm allows you to optimize the length of the microstrip patch antenna resulting in increased gain and reduced return loss. Bakelite is used as a substrate. The width of the patch antenna is set according to the most suitable length selected. To increase the bandwidth and Voltage Standing Wave Ratio (VSWR), a square split ring resonator (SSRR) is used as a metamaterial. An evaluation of the designed microstrip patch antenna model with existing patch antennas was performed. The estimated values of the parameters of the proposed model were the following values: return loss –72.54 dB, resonant frequency 1.11 THz, achieved gain 15.25 dB, VSWR value 1.5646. The estimated values of the developed model exceed those of existing samples. Thus, the developed microstrip patch antenna using Fennec Fox optimization and square split ring resonator metamaterial shows better results in the terahertz range.

Abstract Background Bruton’s tyrosine kinase (Btk) is an enzyme expressed in leukocytes other than T lymphocytes and plasma cells and involved in B-cell receptor- and Fcγ receptor (FcγR)-mediated signal transduction. Btk inhibitors potentially suppress autoantibody production due to the expected inhibitory ability of B lymphocyte differentiation into antibody-producing plasma cells and reduce FcγR-mediated neutrophil activation, including the release of neutrophil extracellular traps (NETs). Microscopic polyangiitis (MPA) is a systemic small-vessel vasculitis characterized by the pathogenic autoantibody, antineutrophil cytoplasmic antibody (ANCA) that reacts with myeloperoxidase (MPO). MPO and MPO-ANCA immune complex (IC)-induced FcγR-mediated NETs are critically involved in MPA pathogenesis. This study aimed to demonstrate the therapeutic efficacy of the Btk inhibitor tirabrutinib on MPA. Methods Various doses of tirabrutinib or vehicle were orally administered to Sprague–Dawley rats daily. Four weeks later, the number of peripheral B lymphocytes was counted, and Btk phosphorylation in B lymphocytes was evaluated by flow cytometry. Human peripheral blood neutrophils were stimulated by MPO and anti-MPO antibody ICs (MPO and anti-MPO-ICs), and Btk and its downstream Vav phosphorylation were assessed by western blotting. The effects of tirabrutinib on MPO and anti-MPO-IC-induced NET formation were examined in vitro. Wistar Kyoto rats were immunized with human MPO to induce experimental MPA and given drug-free or tirabrutinib-containing feed (0.0037% or 0.012%) from day 0 or 28. All rats were euthanized on day 42 for serological and histological evaluation. Results Tirabrutinib inhibited Btk phosphorylation without decreasing B lymphocytes in vivo. Neutrophil Btk and Vav were phosphorylated when stimulated with MPO and anti-MPO-ICs. Tirabrutinib suppressed MPO and anti-MPO-IC-induced NET formation in vitro and ameliorated experimental MPA in a dose-dependent manner in vivo. Although MPO-ANCA production was not affected, NET-forming neutrophils in the blood were significantly reduced by tirabrutinib. Conclusions The Btk inhibitor tirabrutinib suppressed MPO and anti-MPO-IC-induced NET formation in vitro and ameliorated experimental MPA by reducing NET-forming neutrophils but not decreasing MPO-ANCA titer in vivo. This study suggests that Btk is a possible therapeutic target in MPA.

Small no-take marine protected areas (MPAs) in urban settings can fail to adequately protect biodiversity due to the combined effects of illegal fishing and species emigrating outside the protected area boundaries. Further assessment of the effectiveness of these areas is needed to provide insights into how they can best be managed to generate conservation benefits. The Fly Point no-take MPA in Port Stephens, New South Wales, Australia, was used as a case study, with the objective of examining whether a small urban no-take MPA can produce conservation benefits, despite the handicaps imposed by its size and location. Diver-based underwater visual census data, recording fish species and abundances, was obtained from 434 surveys conducted in Port Stephens (2009–2022) at three sites within the Fly Point no-take MPA and at three surrounding sites open to fishing. These data were analysed using permutational multivariate analysis of variance to determine whether no-take protection significantly benefited fish species richness and diversity. We found significantly higher species richness for sites in the no-take MPA than in surrounding areas and significant differences in assemblages between no-take and fished areas, driven in part by greater abundances of two fishery-targeted species in the no-take MPA (Acanthopagrus australis and Scorpis lineolata). Generally, fish diversity was also significantly higher for sites within the no-take MPA, although diversity was also high in fished sites adjacent to the no-take MPA. Study results demonstrate that small urban no-take MPAs can provide conservation benefits, especially when these areas have been protected for more than a decade and where high visibility and local stewardship enable adequate enforcement of no-take restrictions. Consequently, planning for MPAs in urban areas should endeavour to ensure high levels of public support and, ideally, should situate MPAs in highly visible locations, in order to maximise their conservation outcomes.

Micro-pyramid copper molds are critical components in the preparation of high-precision optical elements, such as light-trapping films and reflective films. Their surfaces are composed of micro-pyramid arrays (MPAs). The surface roughness and edge burrs of MPAs seriously affect the optical properties of optical elements. To reduce the surface roughness, as well as the sizes of the edge burrs, the longitudinal ultrasonic vibration-assisted planing (LUVP) method for processing MPAs was developed during this study. In addition, an experiment was conducted to compare the precision planing and LUVP methods of MPA generation. The results show that the tool nose amplitude of the LUVP experimental platform constructed during this study was 3.3 μm, and that the operating frequency was 19.85 kHz. An MPA processed by LUVP had a smaller surface roughness than that of an MPA produced by precision planing; it also had fewer and smaller edge burrs, and there was slightly less diamond tool wear. The MPA cut using the LUVP method had no corrugation on its surface. This research lays a foundation for developing higher-precision micro-pyramid plastic films.

Background. Cleaning symbiotic interactions are an important component of coral reef biodiversity and the study of the characteristics of these interacting species networks allows to assess the health of communities. The coral reefs of Jardines de la Reina National Park (JRNP) are subject to a protection gradient and there is a lack of knowledge about the effect of different levels of protection on the cleaning mutualistic networks in the area. The present study aims to characterize the mutualistic cleaning networks in the reefs of JRNP and to assess the potential effect of the protection gradient on their characteristics. Methods. We visited 26 reef sites distributed along the National Park and performed 96 band transects (50 m × 4 m). Low, medium and highly protected regions were compared according to the number of cleaning stations and the abundance and number of species of clients and cleaners associated with them. Additionally, we built interaction networks for the three regions and the entire archipelago based on a total of 150 minutes' video records of active cleaning stations. Weassessed ecological networks characteristics (specialization, nestedness) using network topological metrics. Results. We found a high diversity and complex cleaning interaction network with 6 cleaner species and 39 client species, among them, the threatened grouper Epinephelus striatus was one of the most common clients. Noclear effect of the protection level on the density, abundance or diversity of cleaners and clients was detected during this study. However, we found that the network structure varied among regions, with the highly protected region being more specialized and less nested than the other regions. Our research reveals some patterns that suggest the effect of fishing pressure on cleaning symbiosis, as fishing may reduce the abundance and composition of client species, especially those that are targeted by fishers. However, fishing pressure may not be the main factor influencing cleaning symbiosis inside of the National Park, as other factors, such as habitat quality or environmental conditions may have stronger effects on the demand for cleaning services and the interactions between cleaners and clients. Our research provides insights into the factors that influence cleaning symbiosis and its implications for coral reef conservation and management. [ABSTRACT FROM AUTHOR]

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Mobile marine protected areas have been proposed for the conservation of highly seasonal or mobile marine megafauna. However, seasonal data on the distribution of marine wildlife to inform protected areas are generally scarce worldwide, especially for cetaceans, which makes dynamic solutions difficult to implement.Furthermore, conservation objectives are often set at the level of individual species rather than at the community level, despite many species having similar or overlapping habitat requirements, and a comparison of the effectiveness of mobile vs. static Marine Protected Areas options has rarely been done.Systematic conservation planning was used to identify priority areas of cetacean biodiversity in the north‐east Atlantic accounting for seasonal changes in distribution. Consistent hotspots across seasons at a community level, in particular along the shelf edge, suggest that fixed priority areas for cetacean biodiversity may be appropriate.The area required for protection to meet conservation targets (i.e. 20% of a population occurring within a protected area) is minimized when considering populations at basin scale rather than national level. Highly mobile megafauna normally exploit persistent and predictable oceanographic features, so a habitat suitability rather than a jurisdiction‐based approach is more appropriate. [ABSTRACT FROM AUTHOR]

Small no-take marine protected areas (MPAs) in urban settings can fail to adequately protect biodiversity due to the combined effects of illegal fishing and species emigrating outside the protected area boundaries. Further assessment of the effectiveness of these areas is needed to provide insights into how they can best be managed to generate conservation benefits. The Fly Point no-take MPA in Port Stephens, New South Wales, Australia, was used as a case study, with the objective of examining whether a small urban no-take MPA can produce conservation benefits, despite the handicaps imposed by its size and location. Diver-based underwater visual census data, recording fish species and abundances, was obtained from 434 surveys conducted in Port Stephens (2009–2022) at three sites within the Fly Point no-take MPA and at three surrounding sites open to fishing. These data were analysed using permutational multivariate analysis of variance to determine whether no-take protection significantly benefited fish species richness and diversity. We found significantly higher species richness for sites in the no-take MPA than in surrounding areas and significant differences in assemblages between no-take and fished areas, driven in part by greater abundances of two fishery-targeted species in the no-take MPA (Acanthopagrus australis and Scorpis lineolata). Generally, fish diversity was also significantly higher for sites within the no-take MPA, although diversity was also high in fished sites adjacent to the no-take MPA. Study results demonstrate that small urban no-take MPAs can provide conservation benefits, especially when these areas have been protected for more than a decade and where high visibility and local stewardship enable adequate enforcement of no-take restrictions. Consequently, planning for MPAs in urban areas should endeavour to ensure high levels of public support and, ideally, should situate MPAs in highly visible locations, in order to maximise their conservation outcomes. [ABSTRACT FROM AUTHOR]

Music Performance Anxiety (MPA) is one of the most widespread and debilitating challenges facing musicians, affecting significant numbers of performers in terms of both their personal and professional functioning. Although numerous interventions exist to target MPA, its prevalence remains unchanged since the first large-scale studies of the 1980s, indicating that available interventions are having limited impact. This review synthesizes and critiques existing literature in order to investigate possible reasons for the limited efficacy of current approaches to managing MPA. Key concepts discussed include conceptual and methodological challenges surrounding defining MPA, theoretical perspectives on MPA's etiology and manifestation, and the coping strategies and interventions used to manage MPA. MPA has predominantly been investigated pathologically and defined as a negative construct manifesting in unwanted symptoms. Based on this conceptualization, interventions largely seek to manage MPA through ameliorating symptoms. This review discusses possible reasons why this approach has broadly not proved successful, including the issue of relaxation being both unrealistic and counterproductive for peak performance, issues associated with intentionally changing one's state creating resistance thus exacerbating anxiety, and focusing on the presence of, rather than response to, symptoms. Despite 50 years of research, MPA remains an unsolved enigma and continues to adversely impact musicians both on and off the stage. Reconceptualizing MPA as a normal and adaptive response to the pressures of performance may offer a new perspective on it, in terms of its definition, assessment and management, with practical as well as theoretical implications. [ABSTRACT FROM AUTHOR]

Mentoring is important for students in a Master of Public Administration (MPA) program. This can be even more complicated when the academic program is fully online. Effective mentoring is about building relationships and guiding academically, professionally and emotionally. This can be challenging in the online environment due to a lack of social cues and technology barriers, but is possible through e-mentoring. This contribution is focused on both the quality of mentorship for MPA students and the complexities of providing mentoring in a fully online environment. Additionally, this involves the centering of mentoring in program delivery and how this is integrated into program goals including the Network of Schools of Public Policy, Affairs, and Administration (NASPAA) competencies. A survey was sent to current and former MPA students in a fully online MPA program to assess student perceptions of the ideal mentor and their achievement of the NASPAA competencies. It was found that there is considerable overlap between some mentorship qualities and perceptions of NASPAA competencies. [ABSTRACT FROM AUTHOR]

Abstract Predicting and measuring changes resulting from marine protected areas (MPAs) has posed a challenge for practitioners, partly because ecosystems are complex and can change in unanticipated ways, but also due to MPA characteristics such as design factors, conservation objectives (COs), and monitoring programs, that can leave little chance of meeting stated goals. We consider these design factors for the Laurentian Channel MPA, a large offshore Canadian protected area established to protect against fishing impacts. Specifically, in this study we evaluated (1) whether it is realistic to expect improvements in the MPA for four previously established taxa‐specific COs, and (2) whether existing scientific surveys are capable of detecting changes in these CO taxa even if they occurred. Three CO species were sampled in scientific multispecies research vessel trawl surveys (Black Dogfish, Smooth Skate, and Northern Wolffish) and a fourth CO, sea pen taxa, were enumerated using seafloor imagery. Simulations indicate that trawl surveys have very little chance of detecting change in the abundance of the three fish species examined, while seafloor imagery data had higher statistical power for sea pen taxa. Moreover, we show that expecting change related to the removal of fishing is unrealistic due to the fact that the MPA was established in an area of minimal fishing pressure. While positive change is unlikely to be induced by the MPA, or be detected if they occurred, this MPA could provide conservation benefits if COs and monitoring approaches were realigned to match the unique features of this area that represents largely unimpacted sensitive benthic habitats.

Background: Sickle cell disease (SCD) is the most common hereditary hemoglobinopathy, which delays growth leading to an altered skeleton and craniofacial pattern. Palatal rugae patterning has been considered the regulator of the development of the palate. The purpose of the research work was to study the morphology of the palate, rugae pattern, and its dimensions in SCD children and compare them with healthy normal children, and to evaluate its role as minor physical anomalies (MPAs). Methods: A cross-sectional case-control study was designed as per STROBE guidelines. The sample comprised 50 children diagnosed with sickle cell disease (Group SCD) and 50 normal healthy children as control (Group C) belonging to the same age group (10–18 years). Dental impressions were made, followed by the pouring of dental casts. The length of the palatal rugae was measured and categorized into primary (>5 mm), secondary (3 mm–5 mm), and fragmentary rugae (

The development of indicators as tools for ecosystem monitoring is a key step in the management of marine protected areas (MPAs). This study uses previously developed sex-specific body condition indices, blubber thickness, and girth, to assess temporal changes in body condition from 2000 to 2015 in harvested Eastern Beaufort Sea (EBS) beluga whales (Delphinapterus leucas). Specifically, the goals were to (1) examine seasonal and inter-annual trends of beluga body condition indicators over the harvest season; (2) evaluate associations of body condition indicators across sexes; and (3) test annual means of each body condition index for correlations to regional scale environmental drivers (i.e., the Pacific decadal oscillation (PDO) and sea-ice minimum (SIM) in the Beaufort Sea). Significant seasonal changes in male blubber thickness and female girth indices demonstrated the importance of short-term seasonal drivers. Whilst inter-annual changes in girth and blubber thickness indices revealed longer-term changes, that were correlated between males and females. Only the male girth index had significant relationships with environmental drivers: a negative relationship with the PDO at a zero-year lag, and a negative relationship with the SIM at a two-year lag. The PDO is believed to capture broader environmental changes occurring at wintering habitats and migration routes of the EBS beluga that reveals connectivity between the EBS beluga home range and body condition metrics. While body condition metrics for harvested EBS belugas are regularly collected under a long-running beluga monitoring program, the interpretation and application to MPA monitoring remains difficult. We recommend the continued collection of both girth and blubber thickness measurements along with the inclusion of Inuvialuit Knowledge and observations to strengthen interpretations and inform monitoring of beluga health.

Background Cleaning symbiotic interactions are an important component of coral reef biodiversity and the study of the characteristics of these interacting species networks allows to assess the health of communities. The coral reefs of Jardines de la Reina National Park (JRNP) are subject to a protection gradient and there is a lack of knowledge about the effect of different levels of protection on the cleaning mutualistic networks in the area. The present study aims to characterize the mutualistic cleaning networks in the reefs of JRNP and to assess the potential effect of the protection gradient on their characteristics. Methods We visited 26 reef sites distributed along the National Park and performed 96 band transects (50 m × 4 m). Low, medium and highly protected regions were compared according to the number of cleaning stations and the abundance and number of species of clients and cleaners associated with them. Additionally, we built interaction networks for the three regions and the entire archipelago based on a total of 150 minutes’ video records of active cleaning stations. We assessed ecological networks characteristics (specialization, nestedness) using network topological metrics. Results We found a high diversity and complex cleaning interaction network with 6 cleaner species and 39 client species, among them, the threatened grouper Epinephelus striatus was one of the most common clients. No clear effect of the protection level on the density, abundance or diversity of cleaners and clients was detected during this study. However, we found that the network structure varied among regions, with the highly protected region being more specialized and less nested than the other regions. Our research reveals some patterns that suggest the effect of fishing pressure on cleaning symbiosis, as fishing may reduce the abundance and composition of client species, especially those that are targeted by fishers. However, fishing pressure may not be the main factor influencing cleaning symbiosis inside of the National Park, as other factors, such as habitat quality or environmental conditions may have stronger effects on the demand for cleaning services and the interactions between cleaners and clients. Our research provides insights into the factors that influence cleaning symbiosis and its implications for coral reef conservation and management.

Music Performance Anxiety (MPA) is one of the most widespread and debilitating challenges facing musicians, affecting significant numbers of performers in terms of both their personal and professional functioning. Although numerous interventions exist to target MPA, its prevalence remains unchanged since the first large-scale studies of the 1980s, indicating that available interventions are having limited impact. This review synthesizes and critiques existing literature in order to investigate possible reasons for the limited efficacy of current approaches to managing MPA. Key concepts discussed include conceptual and methodological challenges surrounding defining MPA, theoretical perspectives on MPA’s etiology and manifestation, and the coping strategies and interventions used to manage MPA. MPA has predominantly been investigated pathologically and defined as a negative construct manifesting in unwanted symptoms. Based on this conceptualization, interventions largely seek to manage MPA through ameliorating symptoms. This review discusses possible reasons why this approach has broadly not proved successful, including the issue of relaxation being both unrealistic and counterproductive for peak performance, issues associated with intentionally changing one’s state creating resistance thus exacerbating anxiety, and focusing on the presence of, rather than response to, symptoms. Despite 50 years of research, MPA remains an unsolved enigma and continues to adversely impact musicians both on and off the stage. Reconceptualizing MPA as a normal and adaptive response to the pressures of performance may offer a new perspective on it, in terms of its definition, assessment and management, with practical as well as theoretical implications.

Seagrass meadows are an important component of coastal ecosystems globally, and they capture and store organic carbon in living biomass and sediments. Geographical estimates of blue carbon in seagrass habitats are regionally biased, with limited information from the Indo-Pacific region, including Indonesia. Seagrass extent in Indonesia is declining rapidly, and it has been suggested that marine protected areas (MPAs) are an important instrument to support protection of seagrass ecosystems and their services. Thus, this study is aimed at quantifying and comparing sedimentary carbon stocks and sources of organic carbon from seagrass meadows located in undisturbed areas outside MPA, disturbed areas outside MPA, and within MPA in three small islands in Indonesia. The sediment carbon stocks from this study ranged from 19.81 to 117.49 Mg C ha−1, with the highest stock measured inside MPA (77.15 ± 1.38 Mg C ha−1), followed by undisturbed outside MPA (36.08 Mg C ha−1), and the lowest stock at disturbed outside MPA (21.86 ± 0.31 Mg C ha−1). The predominant source of organic carbon in disturbed meadows was from coastal POM (particulate organic matter, ~ 36%), while in MPA and undisturbed sites, the main source was from seagrass, with ~ 38% and ~ 60% contributions, respectively. The results of this study add more data and information on seagrass blue carbon potential from three different islands with different degrees of disturbance in Indonesia. [ABSTRACT FROM AUTHOR]

This study provided a preliminary comparative analysis of meiobenthic assemblages in different habitats of the Snake Island Marine Protected Area (MPA). Meiobenthos was studied in three habitats: Exposed Black Sea upper infralittoral rock with Corallinales turf (MB142), Mytilid dominated exposed Black Sea upper infralittoral rock with foliose algae (no Fucales) (MB143) and Black Sea infralittoral sand and muddy sand without macroalgae (MB542). A total of 10 higher meiobenthos taxa were recorded in the different habitats: 5 permanent groups (Platyhelminthes, Nematoda, Harpacticoida (Copepoda), Ostracoda, Halacaridae) and 5 temporary groups (Oligochaeta, Polychaeta, Bivalvia, Gastropoda, Cyrripedia). The preliminary study of meiobenthos in the habitats of the Snake Island MPA showed that habitat MB143 was more favourable for meiobenthos, with the highest mean density and biomass (190 655 ± 23 004 ind.-m-2 and 3170.90 ± 412.15 mg-m-2, respectively). The comparative nMDS and cluster analyses based on the density of meiobenthos taxa in the Snake Island MPA showed that the meiofauna of both MB142 and MB143 differed significantly from the MB542 habitat, with Bivalvia and Ostracoda making the largest percentage contribution to these differences. The meiobenthos assemblages in the different habitats of the Snake Island MPA were similar to the other regions of the Black Sea with some unique features (e. g. relatively lower total meiobenthos density, high percentage of ostracodes on the algal substrate, etc.). Further studies on the characteristics of meiobenthos taxa assemblages in other Black Sea MPAs could lead to the estimation of meiobenthos assemblage patterns and their possible use in biomonitoring. [ABSTRACT FROM AUTHOR]

Elasmobranchs are threatened and eDNA metabarcoding is a powerful tool that can help efforts to better understand and conserve them. Nevertheless, the inter‐calibration between optimal methodological practices and its implementation in resource‐limited situations is still an issue. Based on promising results from recent studies, the authors applied a cost‐effective protocol with parameters that could be easily replicated by any conservationist. Nonetheless, the results with fewer elasmobranchs detected than expected reveal that endorsed primers and sampling strategies still require further optimization, especially for applications in resource‐limited conservation programmes. [ABSTRACT FROM AUTHOR]