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1. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Author

Joshua A. Welsh, Deborah C. I. Goberdhan, Lorraine O'Driscoll, Edit I. Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A. P. Driedonks, Uta Erdbrügger, Juan M. Falcon‐Perez, Qing‐Ling Fu, Andrew F. Hill, Metka Lenassi, Sai Kiang Lim, Mỹ G. Mahoney, Sujata Mohanty, Andreas Möller, Rienk Nieuwland, Takahiro Ochiya, Susmita Sahoo, Ana C. Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich, Reem Bagabas, Paolo Bergese, Esther M. Bridges, Marco Brucale, Dylan Burger, Randy P. Carney, Emanuele Cocucci, Rossella Crescitelli, Edveena Hanser, Adrian L. Harris, Norman J. Haughey, An Hendrix, Alexander R. Ivanov, Tijana Jovanovic‐Talisman, Nicole A. Kruh‐Garcia, Vroniqa Ku'ulei‐Lyn Faustino, Diego Kyburz, Cecilia Lässer, Kathleen M. Lennon, Jan Lötvall, Adam L. Maddox, Elena S. Martens‐Uzunova, Rachel R. Mizenko, Lauren A. Newman, Andrea Ridolfi, Eva Rohde, Tatu Rojalin, Andrew Rowland, Andras Saftics, Ursula S. Sandau, Julie A. Saugstad, Faezeh Shekari, Simon Swift, Dmitry Ter‐Ovanesyan, Juan P. Tosar, Zivile Useckaite, Francesco Valle, Zoltan Varga, Edwin van derPol, Martijn J. C. vanHerwijnen, Marca H. M. Wauben, Ann M. Wehman, Sarah Williams, Andrea Zendrini, Alan J. Zimmerman, MISEV Consortium, Clotilde Théry, and Kenneth W. Witwer

Subjects
ectosomes, exosomes, extracellular vesicles, extracellular particles, guidelines, microparticles, Cytology, QH573-671
Abstract

Abstract Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non‐vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Published
2024
Full Text
View/download PDF

2. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Author

Welsh, Joshua A., Goberdhan, Deborah C. I., O’Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A. P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing-Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, M˜y, G. Mahoney, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Hendrix, An, Ivanov, Alexander R., Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A., Faustino, Vroniqa Ku’ulei-Lyn, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M., Lötvall, Jan, Maddox, Adam L., Martens-Uzunova, Elena S., Mizenko, Rachel R., Newman, Lauren A., Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S., Saugstad, Julie A., Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P., Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J. C., Wauben, Marca H. M., Wehman, Ann M., Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J., MISEV Consortium, Thery, Clotilde, Witwer, Kenneth W., Welsh, Joshua A., Goberdhan, Deborah C. I., O’Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A. P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing-Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, M˜y, G. Mahoney, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Hendrix, An, Ivanov, Alexander R., Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A., Faustino, Vroniqa Ku’ulei-Lyn, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M., Lötvall, Jan, Maddox, Adam L., Martens-Uzunova, Elena S., Mizenko, Rachel R., Newman, Lauren A., Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S., Saugstad, Julie A., Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P., Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J. C., Wauben, Marca H. M., Wehman, Ann M., Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J., MISEV Consortium, Thery, Clotilde, and Witwer, Kenneth W.

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly., Godkänd;2024;Nivå 0;2024-04-05 (marisr);Full text license: CC BY

Published
2024
Full Text
View/download PDF

3. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Author

Welsh, Joshua A, Goberdhan, Deborah C I, O'Driscoll, Lorraine, Buzas, Edit I, Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A P, Erdbrügger, Uta, Falcon-Perez, Juan M, Fu, Qing-Ling, Hill, Andrew F, Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C, Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M, Brucale, Marco, Burger, Dylan, Carney, Randy P, Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L, Haughey, Norman J, Hendrix, An, Ivanov, Alexander R, Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A, Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M, Lötvall, Jan, Maddox, Adam L, Martens-Uzunova, Elena S, Mizenko, Rachel R, Newman, Lauren A, Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S, Saugstad, Julie A, Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P, Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J C, Wauben, Marca H M, Wehman, Ann M, Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, null, Théry, Clotilde, Witwer, Kenneth W, Stoorvogel, Willem, Welsh, Joshua A, Goberdhan, Deborah C I, O'Driscoll, Lorraine, Buzas, Edit I, Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A P, Erdbrügger, Uta, Falcon-Perez, Juan M, Fu, Qing-Ling, Hill, Andrew F, Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C, Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M, Brucale, Marco, Burger, Dylan, Carney, Randy P, Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L, Haughey, Norman J, Hendrix, An, Ivanov, Alexander R, Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A, Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M, Lötvall, Jan, Maddox, Adam L, Martens-Uzunova, Elena S, Mizenko, Rachel R, Newman, Lauren A, Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S, Saugstad, Julie A, Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P, Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J C, Wauben, Marca H M, Wehman, Ann M, Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, null, Théry, Clotilde, Witwer, Kenneth W, and Stoorvogel, Willem

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Published
2024

4. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Author

CDL extracellular vesicles, MS Nefrologie, Circulatory Health, CDL Nanomedicine, CDL Staf Research, CMM Groep Coffer, Cancer, Regenerative Medicine and Stem Cells, Zorgeenheid MKA/BT Medisch, MKA/BT Onderzoek, Experimentele Afd. Cardiologie 2, Centraal Diagnostisch Laboratorium, Experimental Cardiology Laboratory, Onderzoek Cardiovasculair Reg. Med., CDL Cluster Onderzoek en Onderwijs, Welsh, Joshua A., Goberdhan, Deborah C.I., O’Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A.P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, M˜y, G. Mahoney, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Hendrix, An, Ivanov, Alexander R., Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A., MISEV Consortium, CDL extracellular vesicles, MS Nefrologie, Circulatory Health, CDL Nanomedicine, CDL Staf Research, CMM Groep Coffer, Cancer, Regenerative Medicine and Stem Cells, Zorgeenheid MKA/BT Medisch, MKA/BT Onderzoek, Experimentele Afd. Cardiologie 2, Centraal Diagnostisch Laboratorium, Experimental Cardiology Laboratory, Onderzoek Cardiovasculair Reg. Med., CDL Cluster Onderzoek en Onderwijs, Welsh, Joshua A., Goberdhan, Deborah C.I., O’Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A.P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, M˜y, G. Mahoney, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Hendrix, An, Ivanov, Alexander R., Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A., and MISEV Consortium

Published
2024

5. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Author

Celbiologie, Cell Biology, Metabolism & Cancer - Cellbiology, Welsh, Joshua A, Goberdhan, Deborah C I, O'Driscoll, Lorraine, Buzas, Edit I, Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A P, Erdbrügger, Uta, Falcon-Perez, Juan M, Fu, Qing-Ling, Hill, Andrew F, Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C, Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M, Brucale, Marco, Burger, Dylan, Carney, Randy P, Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L, Haughey, Norman J, Hendrix, An, Ivanov, Alexander R, Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A, Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M, Lötvall, Jan, Maddox, Adam L, Martens-Uzunova, Elena S, Mizenko, Rachel R, Newman, Lauren A, Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S, Saugstad, Julie A, Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P, Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J C, Wauben, Marca H M, Wehman, Ann M, Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, Théry, Clotilde, Witwer, Kenneth W, Stoorvogel, Willem, Celbiologie, Cell Biology, Metabolism & Cancer - Cellbiology, Welsh, Joshua A, Goberdhan, Deborah C I, O'Driscoll, Lorraine, Buzas, Edit I, Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A P, Erdbrügger, Uta, Falcon-Perez, Juan M, Fu, Qing-Ling, Hill, Andrew F, Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C, Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M, Brucale, Marco, Burger, Dylan, Carney, Randy P, Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L, Haughey, Norman J, Hendrix, An, Ivanov, Alexander R, Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A, Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M, Lötvall, Jan, Maddox, Adam L, Martens-Uzunova, Elena S, Mizenko, Rachel R, Newman, Lauren A, Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S, Saugstad, Julie A, Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P, Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J C, Wauben, Marca H M, Wehman, Ann M, Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, Théry, Clotilde, Witwer, Kenneth W, and Stoorvogel, Willem

Published
2024

6. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches.

Author

Welsh, JA, Goberdhan, DCI, O'Driscoll, L, Buzas, EI, Blenkiron, C, Bussolati, B, Cai, H, Di Vizio, D, Driedonks, TAP, Erdbrügger, U, Falcon-Perez, JM, Fu, Q-L, Hill, AF, Lenassi, M, Lim, SK, Mahoney, MG, Mohanty, S, Möller, A, Nieuwland, R, Ochiya, T, Sahoo, S, Torrecilhas, AC, Zheng, L, Zijlstra, A, Abuelreich, S, Bagabas, R, Bergese, P, Bridges, EM, Brucale, M, Burger, D, Carney, RP, Cocucci, E, Crescitelli, R, Hanser, E, Harris, AL, Haughey, NJ, Hendrix, A, Ivanov, AR, Jovanovic-Talisman, T, Kruh-Garcia, NA, Ku'ulei-Lyn Faustino, V, Kyburz, D, Lässer, C, Lennon, KM, Lötvall, J, Maddox, AL, Martens-Uzunova, ES, Mizenko, RR, Newman, LA, Ridolfi, A, Rohde, E, Rojalin, T, Rowland, A, Saftics, A, Sandau, US, Saugstad, JA, Shekari, F, Swift, S, Ter-Ovanesyan, D, Tosar, JP, Useckaite, Z, Valle, F, Varga, Z, van der Pol, E, van Herwijnen, MJC, Wauben, MHM, Wehman, AM, Williams, S, Zendrini, A, Zimmerman, AJ, MISEV Consortium, Théry, C, Witwer, KW, Welsh, JA, Goberdhan, DCI, O'Driscoll, L, Buzas, EI, Blenkiron, C, Bussolati, B, Cai, H, Di Vizio, D, Driedonks, TAP, Erdbrügger, U, Falcon-Perez, JM, Fu, Q-L, Hill, AF, Lenassi, M, Lim, SK, Mahoney, MG, Mohanty, S, Möller, A, Nieuwland, R, Ochiya, T, Sahoo, S, Torrecilhas, AC, Zheng, L, Zijlstra, A, Abuelreich, S, Bagabas, R, Bergese, P, Bridges, EM, Brucale, M, Burger, D, Carney, RP, Cocucci, E, Crescitelli, R, Hanser, E, Harris, AL, Haughey, NJ, Hendrix, A, Ivanov, AR, Jovanovic-Talisman, T, Kruh-Garcia, NA, Ku'ulei-Lyn Faustino, V, Kyburz, D, Lässer, C, Lennon, KM, Lötvall, J, Maddox, AL, Martens-Uzunova, ES, Mizenko, RR, Newman, LA, Ridolfi, A, Rohde, E, Rojalin, T, Rowland, A, Saftics, A, Sandau, US, Saugstad, JA, Shekari, F, Swift, S, Ter-Ovanesyan, D, Tosar, JP, Useckaite, Z, Valle, F, Varga, Z, van der Pol, E, van Herwijnen, MJC, Wauben, MHM, Wehman, AM, Williams, S, Zendrini, A, Zimmerman, AJ, MISEV Consortium, Théry, C, and Witwer, KW

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Published
2024

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