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Using the hanging drop technique with nematode growth gelrite medium, life history traits of Steinernema yirgalemense (strain Sy 157-C) were investigated at a bacterial density of 10 × 109 cells ml-1 of Xenorhabdus indica at 25°C. With the same technique, the exit of dauer juveniles (DJ) from the arrested stage (recovery) was assessed at 5 × 109, 10 × 109 and 20 × 109 cells ml-1 of X. indica. Additionally, S. yirgalemense was incubated in nematode liquid medium at 25, 27 and 30°C. At each culture temperature, DJ recovery, sex ratio at 3 days post DJ inoculation and DJ yield and DJ as a percentage of non-DJ stages at 15 days post DJ inoculation were assessed. DJ survival in Ringer's solution stored at 4, 15 and 25°C was assessed for 66 days. Steinernema yirgalemense has a total fertility rate and net reproductive rate of 487 and 314 offspring per female, respectively. The intrinsic rate of natural increase rm was 0.98 day-1, population doubling time PDT = 0.71 days and mean generation time T = 5.72 days. The average lifespan of S. yirgalemense females starting from first-stage juveniles was 6.55 days. In liquid culture, DJ recovery ranged from 63- 75% at 72 h post DJ inoculation and was not significantly different between the incubation temperatures. Parental male to female ratio was not influenced by incubation temperature and usually was at a ratio of 1:2. The percentage of females that entered into endotokia matricida at 72 h post DJ inoculation was 61% at 25°C, whereas at 27 and 30°C it was 24% and 0.5%, respectively. The highest DJ yield was recorded at 25°C (284 114 DJ ml-1) followed by 27°C (176 932 DJ ml-1) and the lowest at 30°C with 26 298 DJ ml-1. At a storage temperature of 4°C, DJ survival did not exceed 42 days, whereas at 15 and 25°C more than 95% of the DJ survived 66 days. Although S. yirgalemense DJ survived for long periods at both 15 and 25°C in liquid storage, their survival in formulated product and virulence after storage needs further investigation. [ABSTRACT FROM AUTHOR]