1. A theranostic agent to enhance osteogenic and magnetic resonance imaging properties of calcium phosphate cements
- Author
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X. Frank Walboomers, Paul J. A. Borm, Sjef Cremers, Arend Heerschap, John A. Jansen, Peter M. van der Kraan, Yi Sun, Manuela Ventura, Zeinab Tahmasebi Birgani, Pamela Habibovic, Division Instructive Biomaterials Eng, RS: MERLN - Instructive Biomaterials Engineering (IBE), and Faculty of Science and Technology
- Subjects
METIS-299570 ,Calcium Phosphates ,Materials science ,Biophysics ,chemistry.chemical_element ,Bioengineering ,Bone healing ,Calcium ,Bone tissue engineering ,Biomaterials ,chemistry.chemical_compound ,In vivo ,Osteogenesis ,Urological cancers Radboud Institute for Molecular Life Sciences [Radboudumc 15] ,Animals ,BMP (bone morphogenetic protein) ,Cement ,Bone Cements ,MRI (magnetic resonance imaging) ,IR-88211 ,X-Ray Microtomography ,Mesoporous silica ,Silane ,Magnetic Resonance Imaging ,In vitro ,Rats ,Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] ,chemistry ,Mechanics of Materials ,Drug delivery ,Ceramics and Composites ,Microscopy, Electron, Scanning ,Calcium phosphate cement ,Inflammatory diseases Radboud Institute for Molecular Life Sciences [Radboudumc 5] ,Biomedical engineering - Abstract
Item does not contain fulltext With biomimetic biomaterials, like calcium phosphate cements (CPCs), non-invasive assessment of tissue regeneration is challenging. This study describes a theranostic agent (TA) to simultaneously enhance both imaging and osteogenic properties of such a bone substitute material. For this purpose, mesoporous silica beads were produced containing an iron oxide core to enhance bone magnetic resonance (MR) contrast. The same beads were functionalized with silane linkers to immobilize the osteoinductive protein BMP-2, and finally received a calcium phosphate coating, before being embedded in the CPC. Both in vitro and in vivo tests were performed. In vitro testing showed that the TA beads did not interfere with essential material properties like cement setting. Furthermore, bioactive BMP-2 could be efficiently released from the carrier-beads. In vivo testing in a femoral condyle defect rat model showed long-term MR contrast enhancement, as well as improved osteogenic capacity. Moreover, the TA was released during CPC degradation and was not incorporated into the newly formed bone. In conclusion, the described TA was shown to be suitable for longitudinal material degradation and bone healing studies.
- Published
- 2014