1. Transforming growth factor beta controls the directional migration of hepatocyte cohorts by modulating their adhesion to fibronectin
- Author
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Urszula Hibner, Fabien Binamé, Patrice Lassus, Institut de Génétique Moléculaire de Montpellier ( IGMM ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Génétique Moléculaire de Montpellier (IGMM), and Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
- Subjects
rac1 GTP-Binding Protein ,MESH : Cell Line ,MESH : Protein Subunits ,Apoptosis ,Cell Communication ,Integrin alpha5 ,MESH : Hepatocytes ,Epithelium ,Mesoderm ,MESH: Hepatocytes ,Mice ,0302 clinical medicine ,Cell Movement ,Transforming Growth Factor beta ,MESH: Fibronectins ,MESH : Cell Movement ,MESH : Fibronectins ,MESH: Animals ,MESH: Cell Movement ,MESH: Mesoderm ,0303 health sciences ,MESH : Gene Expression Regulation ,MESH: Cell Surface Extensions ,MESH : Mesoderm ,Articles ,Adhesion ,MESH: Protein Subunits ,MESH: Gene Expression Regulation ,Cell biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Hepatocyte ,MESH: Integrin alpha5 ,MESH : Cell Communication ,MESH : Cell Membrane ,MESH : Cell Surface Extensions ,Biology ,Cell Line ,MESH: Cell Adhesion ,MESH: Gene Expression Profiling ,03 medical and health sciences ,MESH : rac1 GTP-Binding Protein ,MESH : Integrin alpha5 ,MESH: Cell Communication ,MESH : Mice ,Cell Adhesion ,medicine ,Animals ,Humans ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,MESH: Mice ,Molecular Biology ,[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,MESH: Transforming Growth Factor beta ,030304 developmental biology ,MESH: Humans ,MESH: rac1 GTP-Binding Protein ,Gene Expression Profiling ,MESH: Apoptosis ,MESH : Gene Expression Profiling ,Cell Membrane ,MESH : Humans ,Cell Biology ,MESH : Epithelium ,Fibronectins ,MESH: Cell Line ,Fibronectin ,Protein Subunits ,MESH: Epithelium ,MESH : Cell Adhesion ,MESH : Transforming Growth Factor beta ,Gene Expression Regulation ,Hepatocytes ,biology.protein ,Cell Surface Extensions ,MESH : Animals ,MESH : Apoptosis ,MESH: Cell Membrane ,Transforming growth factor - Abstract
International audience; Transforming growth factor beta (TGF-beta) has a strong impact on liver development and physiopathology, exercised through its pleiotropic effects on growth, differentiation, survival, and migration. When exposed to TGF-beta, the mhAT3F cells, immortalized, highly differentiated hepatocytes, maintained their epithelial morphology and underwent dramatic alterations of adhesion, leading to partial or complete detachment from a culture plate, followed by readhesion and spreading. These alterations of adhesive behavior were caused by sequential changes in expression of the alpha5beta1 integrin and of its ligand, the fibronectin. The altered specificity of anchorage to the extracellular matrix gave rise to changes in cells' collective motility: cohorts adhering to fibronectin maintained a persistent, directional motility, with ezrin-rich pathfinder cells protruding from the tips of the cohorts. The absence of adhesion to fibronectin prevented the appearance of polarized pathfinders and lead to random, oscillatory motility. Our data suggest a novel role for TGF-beta in the control of collective migration of epithelial cohorts.
- Published
- 2008
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