Descriptor: "MESH : Image Processing, Computer-Assisted" - Searchworks@Jio Institute Digital Library Search Results

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27 results on '"MESH : Image Processing, Computer-Assisted"'

1. Genetic and Environmental Influences on the Visual Word Form and Fusiform Face Areas

Author: Stanislas Dehaene, Ghislaine Dehaene-Lambertz, Fabien Fauchereau, Thomas Bourgeron, Eric Artiges, Cyril Poupon, Denis Le Bihan, Philippe Pinel, Christophe Lalanne, Neuroimagerie cognitive - Psychologie cognitive expérimentale (UNICOG-U992), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Saclay (COmUE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Chaire Psychologie cognitive expérimentale, Collège de France (CdF (institution)), Recherche Clinique ville-hôpital, Méthodologies et Société (REMES), Université Paris Diderot - Paris 7 (UPD7), Trouble du comportement alimentaire de l'adolescent, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Université Paris-Sud - Paris 11 (UP11), Gènes, Synapses et Cognition (CNRS - UMR3571 ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Fondation FondaMental [Créteil], Génétique humaine et fonctions cognitives - Human Genetics and Cognitive Functions (GHFC (UMR_3571 / U-Pasteur_1)), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Service NEUROSPIN (NEUROSPIN), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Neuroimagerie en psychiatrie (U1000), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Sud - Paris 11 (UP11), This work was supported by Institut National de la Santé et de la Recherche Médicale (INSERM), Commissariat à l'Energie Atomique (CEA) and the James S. McDonnell Foundation., Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Collège de France - Chaire Psychologie cognitive expérimentale, Université Paris-Sud - Paris 11 (UP11)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neuroimagerie cognitive ( UNICOG-U992 ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris-Sud - Paris 11 ( UP11 ), Collège de France ( CDF ), Collège de France ( CdF ), Recherche Clinique ville-hôpital, Méthodologies et Société ( REMES ), Université Paris Diderot - Paris 7 ( UPD7 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Université Paris-Sud - Paris 11 ( UP11 ), Gènes, Synapses et Cognition, Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 ( UPD7 ) -Centre National de la Recherche Scientifique ( CNRS ), Génétique humaine et Fonctions cognitives - Human Genetics and Cognitive Functions, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Service NEUROSPIN ( NEUROSPIN ), Direction de Recherche Fondamentale (CEA) ( DRF (CEA) ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay, Neuroimagerie en psychiatrie ( U1000 ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ), Neuroimagerie cognitive (UNICOG-U992), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Sud - Paris 11 (UP11), Collège de France (CDF), Collège de France (CdF), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut Pasteur [Paris], and Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)
Subjects: Male, MESH : Oxygen, MESH : Brain Mapping, [ SDV.MHEP.PSM ] Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, [SDV]Life Sciences [q-bio], Statistics as Topic, MESH : Statistics as Topic, temporal, MESH : Photic Stimulation, Functional Laterality, MESH: Magnetic Resonance Imaging, [SCCO]Cognitive science, 0302 clinical medicine, Face perception, Stress (linguistics), Image Processing, Computer-Assisted, Twins, Dizygotic, Visual word form area, 10. No inequality, MESH: Brain Mapping, Language, Brain Mapping, medicine.diagnostic_test, 05 social sciences, MESH: Gene-Environment Interaction, MESH : Adult, Magnetic Resonance Imaging, MESH: Image Processing, Computer-Assisted, Temporal Lobe, MESH : Twins, Monozygotic, MESH: Photic Stimulation, Pattern Recognition, Visual, MESH: Young Adult, MESH: Language, Visual Perception, [ SCCO ] Cognitive science, MESH: Twins, Monozygotic, Psychology, MESH : Visual Perception, MESH : Image Processing, Computer-Assisted, MESH: Oxygen, VWFA, Cognitive psychology, Adult, MESH : Twins, Dizygotic, MESH : Male, Cognitive Neuroscience, MESH : Young Adult, MESH: Pattern Recognition, Visual, 050105 experimental psychology, Lateralization of brain function, Temporal lobe, Young Adult, 03 medical and health sciences, Cellular and Molecular Neuroscience, MESH : Language, reading, MESH : Magnetic Resonance Imaging, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, medicine, Humans, MESH: Temporal Lobe, 0501 psychology and cognitive sciences, MESH: Functional Laterality, MESH : Functional Laterality, MESH : Pattern Recognition, Visual, MESH : Temporal Lobe, MESH: Statistics as Topic, MESH: Humans, [ SDV ] Life Sciences [q-bio], MESH: Visual Perception, MESH: Twins, Dizygotic, MESH : Humans, MESH: Adult, Twins, Monozygotic, Fusiform face area, MESH: Male, MESH : Gene-Environment Interaction, Oxygen, [SDV.MHEP.PSM]Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, Gene-Environment Interaction, genetic, FFA, Functional magnetic resonance imaging, Photic Stimulation, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030217 neurology & neurosurgery, Spoken language
Abstract: International audience; Two areas of the occipitotemporal cortex show a remarkable hemispheric lateralization: written words activate the visual word form area (VWFA) in the left fusiform gyrus and faces activate a symmetrical site in the right hemisphere, the fusiform face area (FFA). While the lateralization of the VWFA fits with the leftward asymmetry of the speech processing network, origin of the rightward asymmetry for faces is still unclear. Using fMRI data from 64 subjects (including 16 monozygotic (MZ) and 13 dizygotic (DZ) twin pairs), we investigated how activations evoked by written words, faces, and spoken language are co-lateralized in the temporal lobe, and whether this organization reflects genetic factors or individual reading expertise. We found that the lateralization of the left superior temporal activation for spoken language correlates with the lateralization of occipitotemporal activations for both written words and faces. Behavioral reading scores also modulate the responses to words and faces. Estimation of genetic and environmental contributions shows that activations of the VWFA, the occipital face area, and the temporal speech areas are partially under genetic control whereas activation of the FFA is primarily influenced by individual experience. Our results stress the importance of both genetic factors and acquired expertise in the occipitotemporal organization.
Published: 2014

2. Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells

Author: Aubin Samacoits, Adham Safieddine, Racha Chouaib, Marion Peter, Florian Mueller, Eugenia Basyuk, Xavier Pichon, Amandine Bastide, Edouard Bertrand, Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Imagerie et Modélisation - Imaging and Modeling, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), A. Safieddine is supported by a Ministère de l'Éducation Nationale de la Recherche et de Technologie fellowship. This work was supported by the Site de Recherche Intégrée sur le Cancer (SIRIC) Montpellier Cancer (grant INCa-DGOS-INSERM 6045) and Fondation de la Recherche Medicale and Agence Nationale de la Recherche HI-FISH grants to E. Basyuk., We thank D. Fisher and A. Camas for the gift of a Gateway mouse Ki67 donor vector, R. Vale for the gift of the SunTag plasmids, I. Poser and T. Hyman for the gift of the DYNC1H1 BAC-GFP cell line, and the Montpellier RIO Imaging facility for help with the imaging experiments., Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut de Génétique Moléculaire de Montpellier ( IGMM ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Imagerie et Modélisation, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), and Mueller, Florian
Subjects: Cytoplasmic Dyneins, 0301 basic medicine, Cytoplasm, Technology, Time Factors, Peptide Chain Elongation, Translational, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Ribosome, MESH : Fluorescence Recovery After Photobleaching, MESH : Cytoplasmic Dyneins, Diffusion, 0302 clinical medicine, MESH : Protein Transport, MESH : Peptide Chain Elongation, Translational, Gene expression, Translational regulation, Image Processing, Computer-Assisted, MESH: Microscopy, Confocal, Spotlight, Ribonucleoprotein, Microscopy, Confocal, Translation (biology), MESH: Diffusion, RNA Biology, MESH: Image Processing, Computer-Assisted, Cell biology, Protein Transport, Ribonucleoproteins, RNA Polymerase II, MESH : RNA Polymerase II, MESH : Transfection, MESH : Image Processing, Computer-Assisted, Fluorescence Recovery After Photobleaching, MESH : Time Factors, MESH : Cytoplasm, MESH: Protein Transport, MESH : Recombinant Fusion Proteins, Recombinant Fusion Proteins, Biophysics, Biology, Transfection, 03 medical and health sciences, Polysome, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], MESH: Recombinant Fusion Proteins, Humans, MESH : HeLa Cells, MESH : Microscopy, Confocal, MESH : Diffusion, MESH: Peptide Chain Elongation, Translational, Messenger RNA, MESH: Humans, MESH: Cytoplasm, MESH: Transfection, MESH : Humans, MESH: Time Factors, [ SDV.BC.BC ] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], MESH: Cytoplasmic Dyneins, RNA, Cell Biology, MESH: RNA Polymerase II, MESH: Ribonucleoproteins, 030104 developmental biology, Polyribosomes, MESH: HeLa Cells, Commentary, MESH : Polyribosomes, MESH : Ribonucleoproteins, MESH: Polyribosomes, 030217 neurology & neurosurgery, MESH: Fluorescence Recovery After Photobleaching, HeLa Cells
Abstract: International audience; Translation is an essential step in gene expression. In this study, we used an improved SunTag system to label nascent proteins and image translation of single messenger ribonucleoproteins (mRNPs) in human cells. Using a dedicated reporter RNA, we observe that translation of single mRNPs stochastically turns on and off while they diffuse through the cytoplasm. We further measure a ribosome density of 1.3 per kilobase and an elongation rate of 13-18 amino acids per second. Tagging the endogenous POLR2A gene revealed similar elongation rates and ribosomal densities and that nearly all messenger RNAs (mRNAs) are engaged in translation. Remarkably, tagging of the heavy chain of dynein 1 (DYNC1H1) shows this mRNA accumulates in foci containing three to seven RNA molecules. These foci are translation sites and thus represent specialized translation factories. We also observe that DYNC1H1 polysomes are actively transported by motors, which may deliver the mature protein at appropriate cellular locations. The SunTag should be broadly applicable to study translational regulation in live single cells.
Published: 2016

3. Autobiographical memory in semantic dementia: new insights from two patients using fMRI

Author: Francis Eustache, Armelle Viard, Vanessa Matuszewski, Serge Belliard, Karine Lebreton, Béatrice Desgranges, Pascale Piolino, Vincent de La Sayette, Neuropsychologie cognitive et neuroanatomie fonctionnelles de la mémoire humaine, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Pontchaillou, CHU Pontchaillou [Rennes], Institut de psychiatrie et neurosciences (U894 / UMS 1266), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Mémoire et Cognition, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the Fondation pour la Recherche sur le Cerveau (FRC)., Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -École pratique des hautes études ( EPHE ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre de Psychiatrie et Neurosciences ( CPN - U894 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), and Eustache, Francis
Subjects: Male, MESH : Oxygen, MESH : Aged, Hippocampal formation, MESH: Magnetic Resonance Imaging, Behavioral Neuroscience, 0302 clinical medicine, MESH: Aged, 80 and over, MESH : Frontotemporal Dementia, Image Processing, Computer-Assisted, MESH : Female, Episodic memory, MESH: Aged, Aged, 80 and over, 05 social sciences, Brain, MESH: Memory Disorders, MESH: Image Processing, Computer-Assisted, Magnetic Resonance Imaging, MESH: Memory, Episodic, MESH : Memory, Episodic, Frontotemporal Dementia, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Female, Psychology, MESH : Image Processing, Computer-Assisted, MESH: Oxygen, Mental image, Cognitive Neuroscience, MESH : Male, Memory, Episodic, Semantic dementia, MESH: Frontotemporal Dementia, Experimental and Cognitive Psychology, 050105 experimental psychology, 03 medical and health sciences, MESH: Brain, Atrophy, Neuroimaging, MESH : Magnetic Resonance Imaging, medicine, MESH : Memory Disorders, Humans, 0501 psychology and cognitive sciences, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Aged, 80 and over, Aged, Memory Disorders, MESH: Humans, Recall, Autobiographical memory, MESH : Humans, medicine.disease, MESH: Male, Oxygen, MESH : Brain, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Neuroscience, MESH: Female, 030217 neurology & neurosurgery
Abstract: International audience; Episodic autobiographical memory (EAM) consists of personal events embedded within a specific spatiotemporal context. Patients with semantic dementia (SD) generally show preserved recent EAMs, but a controversy remains concerning their ability to retrieve remote ones. Only one fMRI study examined remote autobiographical memory in SD through a longitudinal case study (Maguire, Kumaran, Hassabis, & Kopelman, 2010). Here, we propose a cross-sectional study to test the hippocampo-neocortical up-regulation hypothesis, through a multimodal approach (gray matter volume, activation, connectivity analyses), directly comparing recent and remote autobiographical memory retrieval and collecting data to asses phenomelogical re-experiencing. EAM retrieval recruits a distributed network of brain regions, notably the hippocampus which is shown to be atrophied in SD, although some studies report no hippocampal atrophy in SD. Using fMRI, we examined recent and remote EAM retrieval in two SD patients with different profiles of hippocampal atrophy, compared to 12 healthy elders (HE). JPL presented severe bilateral hippocampal atrophy, while EP showed sparing of both hippocampi. Behaviourally, JPL was impaired at retrieving EAMs from both life periods and showed poorer use of visual mental imagery than HE, while EP retrieved memories which were as episodic as those of HE for both periods and relied on greater use of visual mental imagery than HE. Neuroimaging results showed that, for JPL, hyperactivations of the residual hippocampal tissue and of frontal, lateral temporal, occipital and parietal cortices did not efficiently compensate his autobiographical memory deficit. EP however presented hyperactivations in similar neocortical regions which appeared to be more efficient in compensating for atrophy elsewhere, since EP's EAM retrieval was preserved. Functional connectivity analyses focusing on the hippocampus showed how the residual hippocampal activity was connected to other brain areas. For JPL, recent autobiographical retrieval was associated with connectivity between the posterior hippocampus and middle occipital gyrus, while for EP, connectivity was detected between the anterior hippocampus and numerous regions (medial temporal, occipital, temporal, frontal, parietal) for both recent and remote periods. These findings suggest that intensification of hippocampal atrophy in SD strongly affects both recent and remote autobiographical recollection. Up-regulation of neocortical regions and functional hippocampal-neocortical connectivity within the autobiographical network may be insufficient to compensate the lifelong episodic memory deficit for patients with extensive hippocampal atrophy.
Published: 2013

4. FISH-quant: automatic counting of transcripts in 3D FISH images

Author: Xavier Darzacq, Adrien Senecal, Edouard Bertrand, Nathalie Ly, Christophe Zimmer, Florian Mueller, Olivier Collin, Eugenia Basyuk, Hervé Marie-Nelly, Katjana Tantale, Imagerie et Modélisation, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie de l'ENS Paris (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique Moléculaire de Montpellier (IGMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle (IGF), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This research was supported by the Agence Nationale de la Recherche (ANR-10-BLAN-1222, ANR-09-PIRI-0024, ANR-10-INTB-1401), Sidaction, the Région Ile-de-France under C'Nano IdF (the Center of Competences in NanoSciences for the Paris region), the Fondation pour la Recherche Médicale en France (FRM) and the Institut Pasteur, We thank J. McNally for editing the manuscript., ANR-09-PIRI-0024,Chromodyn(2009), ANR-10-BLAN-1222,IMAGenEx,Imagerie de l'expression génétique dans la cellule(2010), ANR-10-INTB-1401,RNAtrans,Visualisation de molécules uniques d'ARNm et de leur topologie afin d'étudier la traduction locale dans les neurones(2010), Mueller, Florian, Programme interdisciplinaire sur les systèmes biologiques et d'innovation biomédicale - - Chromodyn2009 - ANR-09-PIRI-0024 - PIRI - VALID, BLANC - Imagerie de l'expression génétique dans la cellule - - IMAGenEx2010 - ANR-10-BLAN-1222 - BLANC - VALID, Programme Blanc International édition 2010 - Visualisation de molécules uniques d'ARNm et de leur topologie afin d'étudier la traduction locale dans les neurones - - RNAtrans2010 - ANR-10-INTB-1401 - Blanc international 2010 - VALID, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Institut de biologie de l'ENS Paris (UMR 8197/1024) ( IBENS ), École normale supérieure - Paris ( ENS Paris ) -École normale supérieure - Paris ( ENS Paris ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Génétique Moléculaire de Montpellier ( IGMM ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Génomique Fonctionnelle ( IGF ), Centre National de la Recherche Scientifique ( CNRS ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Montpellier 1 ( UM1 ) -Université de Montpellier ( UM ), ANR-09-PIRI-0024,Chromodyn,Mapping and modelling of chromosome dynamics ( 2009 ), ANR-10-BLAN-1222,IMAGenEx,Imagerie de l'expression génétique dans la cellule ( 2010 ), ANR-10-INTB-1401,RNAtrans,Visualisation de molécules uniques d'ARNm et de leur topologie afin d'étudier la traduction locale dans les neurones ( 2010 ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), and Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS)
Subjects: MESH : RNA, Messenger, Transcription, Genetic, [SDV]Life Sciences [q-bio], MESH : Protein Subunits, MESH : Actins, MESH : Models, Biological, Biochemistry, 0302 clinical medicine, Image Processing, Computer-Assisted, MESH: In Situ Hybridization, Fluorescence, In Situ Hybridization, Fluorescence, Genetics, 0303 health sciences, medicine.diagnostic_test, Fluorescence in situ hybridization, MESH: Protein Subunits, MESH: Image Processing, Computer-Assisted, MESH : In Situ Hybridization, Fluorescence, [SDV] Life Sciences [q-bio], MESH: Reproducibility of Results, MESH : Computer Simulation, MESH : Imaging, Three-Dimensional, MESH : RNA Polymerase II, RNA Polymerase II, MESH : Image Processing, Computer-Assisted, Biotechnology, Computational biology, MESH: Imaging, Three-Dimensional, Biology, MESH: Actins, Models, Biological, 03 medical and health sciences, Imaging, Three-Dimensional, MESH: Computer Simulation, medicine, Computer Simulation, RNA, Messenger, Molecular Biology, Gene, 030304 developmental biology, MESH: RNA, Messenger, Messenger RNA, [ SDV ] Life Sciences [q-bio], MESH : Reproducibility of Results, MESH: Transcription, Genetic, MESH: Models, Biological, RNA, MESH : Transcription, Genetic, Reproducibility of Results, Cell Biology, Actins, Computational biology and bioinformatics, MESH: RNA Polymerase II, Protein Subunits, 030217 neurology & neurosurgery
Abstract: International audience; Transcription is inherently stochastic even in clonal cell populations 1. Studies at single-cell-single-molecule level enable a quantitative understanding of the underlying regulatory mechanisms 2,3. A widely used technique is single-molecule RNA fluorescence in-situ hybridization (FISH), in which fluorescent probes target the mRNA of interest and individual molecules appear as bright diffraction-limited spots (Fig. 1a,b) 3. Recent experimental progress makes FISH easy to use 4 , but a dedicated image analysis tool is currently missing. Available methods allow counting of isolated mature mRNAs but cannot reliably quantify the dense mRNA aggregates at transcription sites (TS) in three dimensions (3D), particularly of highly transcribing genes 4. We developed FISH-QUANT to close this gap (Supplementary Note 1)
Published: 2013

5. MRI, FDG-TEP and Florbetapir-TEP in AD

Author: La Joie , Renaud, Perrotin , Audrey, Barre , Louisa, Hommet , Caroline, Mézenge , Florence, Ibazizene , Méziane, Camus , Vincent, Landeau , Brigitte, GUILLOTEAU , Denis, De La Sayette , Vincent, Eustache , Francis, Desgranges , Beatrice, Gaël , Chételat, Neuropsychologie cognitive et neuroanatomie fonctionnelles de la mémoire humaine, Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -École pratique des hautes études ( EPHE ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire de Développements Méthodologiques en Tomographie par Emission de Positions ( LDM-TEP ), Imagerie et Stratégies Thérapeutiques des pathologies Cérébrales et Tumorales ( ISTCT ), Normandie Université ( NU ) -Normandie Université ( NU ) -Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), Centre Mémoire Ressources Recherche ( CMRR ), Hôpital Bretonneau-CHRU Tours, Imagerie et cerveau, Université de Tours-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Clinique Psychiatrique Universitaire, CHRU Tours, Laboratoire de Médecine Nucléaire, Unité de Neurologie Générale ( UNG ), CHU Caen-Pôle Neuro-Cardio Sciences et Urgences, This study was funded by Agence Nationale de la Recherche (ANR LONGVIE 2007), Programme Hospitalier de Recherche Clinique (PHRC National 2008), Région Basse Normandie, and Institut National de la Santé et de la Recherche Médicale (Inserm), including the Inserm Liliane Bettencourt School (MD-PhD Program)., Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Développements Méthodologiques en Tomographie par Emission de Positons (LDM-TEP), Service Hospitalier Frédéric Joliot (SHFJ), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Imagerie et Stratégies Thérapeutiques des pathologies Cérébrales et Tumorales (ISTCT), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Centre Mémoire Ressources Recherche (CMRR), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Hôpital Bretonneau, Imagerie et cerveau (iBrain - Inserm U1253 - UNIV Tours ), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Service de Neurologie [CHU Caen], Normandie Université (NU)-Normandie Université (NU)-CHU Caen, and Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)
Subjects: MESH: Hippocampus, MESH : Male, MESH : Aged, MESH : Alzheimer Disease, MESH : Brain Chemistry, MESH: Atrophy, MESH : Hippocampus, MESH : Amyloid beta-Peptides, MESH: Magnetic Resonance Imaging, MESH : Magnetic Resonance Imaging, MESH : Neuroimaging, MESH : Female, MESH: Neuroimaging, MESH: Brain Chemistry, MESH : Atrophy, MESH: Aged, MESH: Humans, MESH : Humans, MESH: Biological Markers, MESH : Positron-Emission Tomography, MESH: Neuropsychological Tests, MESH: Image Processing, Computer-Assisted, MESH: Male, MESH: Positron-Emission Tomography, MESH: Amyloid beta-Peptides, MESH : Neuropsychological Tests, MESH : Biological Markers, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Neurofibrillary Tangles, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Educational Status, MESH: Female, MESH: Alzheimer Disease, MESH : Image Processing, Computer-Assisted, MESH : Educational Status, MESH: Neurofibrillary Tangles
Abstract: International audience; Gray matter atrophy, glucose hypometabolism, and β-amyloid Aβ deposition are well-described hallmarks of Alzheimer's disease, but their relationships are poorly understood. The present study aims to compare the local levels of these three alterations in humans with Alzheimer's disease. Structural magnetic resonance imaging, (18)F-fluorodeoxyglucose positron emission tomography (PET), and (18)F-florbetapir PET data from 34 amyloid-negative healthy controls and 20 demented patients with a high probability of Alzheimer's disease etiology (attested using neuroimaging biomarkers as recently recommended) were analyzed. For each patient and imaging modality, age-adjusted Z-score maps were computed, and direct between-modality voxelwise comparison and correlation analyses were performed. Significant differences in the levels of atrophy, hypometabolism, and Aβ deposition were found in most brain areas, but the hierarchy differed across regions. A cluster analysis revealed distinct subsets of regions: (1) in the hippocampus, atrophy exceeded hypometabolism, whereas Aβ load was minimal; (2) in posterior association areas, Aβ deposition was predominant, together with high hypometabolism and lower but still significant atrophy; and (3) in frontal regions, Aβ deposition was maximal, whereas structural and metabolic alterations were low. Atrophy and hypometabolism significantly correlated in the hippocampus and temporo-parietal cortex, whereas Aβ load was not significantly related to either atrophy or hypometabolism. These findings provide direct evidence for regional variations in the hierarchy and relationships between Aβ load, hypometabolism, and atrophy. Altogether, these variations probably reflect the differential involvement of region-specific pathological or protective mechanisms, such as the presence of neurofibrillary tangles, disconnection, as well as compensation processes.
Published: 2012

6. An adapted optical flow algorithm for robust quantification of cardiac wall motion from standard cine-MR examinations

Author: François Brunotte, Paul Walker, L. Legrand, M. Xavier, Alain Lalande, Laboratoire Electronique, Informatique et Image ( Le2i ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Lalande, Alain, Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire Electronique, Informatique et Image [UMR6306] (Le2i), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Arts et Métiers (ENSAM), Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-Arts et Métiers Sciences et Technologies, and HESAM Université (HESAM)-HESAM Université (HESAM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement
Subjects: Male, Brightness, Physics::Medical Physics, [INFO.INFO-IM] Computer Science [cs]/Medical Imaging, 02 engineering and technology, 030218 nuclear medicine & medical imaging, motion estimation, 0302 clinical medicine, Cine-MRI, 0202 electrical engineering, electronic engineering, information engineering, Image Processing, Computer-Assisted, Computer vision, MESH : Female, Optical filter, MESH : Algorithms, Mathematics, MESH: Middle Aged, [ INFO.INFO-IM ] Computer Science [cs]/Medical Imaging, General Medicine, MESH: Magnetic Resonance Imaging, Cine, Middle Aged, MESH : Adult, MESH : Magnetic Resonance Imaging, Cine, MESH: Image Processing, Computer-Assisted, Computer Science Applications, cardiovascular system, 020201 artificial intelligence & image processing, Female, Algorithms, MESH : Image Processing, Computer-Assisted, Biotechnology, Adult, MESH : Male, Optical flow, Magnetic Resonance Imaging, Cine, Image processing, MESH: Algorithms, heart, optical flow, 03 medical and health sciences, MESH : Heart, Robustness (computer science), Motion estimation, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Humans, MESH : Middle Aged, Electrical and Electronic Engineering, Centimeter, MESH: Humans, Pixel, business.industry, MESH : Humans, MESH: Adult, MESH: Male, MESH: Heart, Artificial intelligence, business, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; This paper presents a method for local myocardial motion estimation from a conventional steady-state free precession cine-MRI sequence using a modified phase-based optical flow (OF) technique. Initially, the technique was tested on synthetic images to evaluate its robustness with regards to Rician noise and to brightness variations. The method was then applied to cardiac images acquired on 11 healthy subjects. Myocardial velocity is measured in centimeter per second in each studied pixel and visualized as colored vectors superimposed on MRI images. The estimated phase-based OF results were compared with a reference OF method and gave similar results on synthetic images, i.e., without a significant difference of the mean angular error. Applied on cine-MRI of normal hearts, the calculated velocities from short-axis images concord with values obtained in the literature. The advantage of the presented method is its robustness with respect to Rician noise and to brightness changes often observed in cine-MRI sequences, and especially with the through-plane movement of the heart. Motion assessment using our method on cine-MR images gives promising results on motion estimation on a pixel-by-pixel basis, leading to a regional measurement of the time-velocity course of myocardial displacement in different segments of the heart wall.
Published: 2012

7. Subjective cognition and amyloid deposition imaging: a Pittsburgh Compound B positron emission tomography study in normal elderly individuals

Author: Audrey Perrotin, Cindee Madison, Amynta O. Hayenga, Elizabeth C. Mormino, William J. Jagust, Neuropsychologie cognitive et neuroanatomie fonctionnelles de la mémoire humaine, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM), The Helen Wills Neuroscience Institute (HWNI), University of California [Berkeley], University of California-University of California, Life Science Division [LBNL Berkeley], Lawrence Berkeley National Laboratory [Berkeley] (LBNL), This study was supported by grant AG027859 from the National Institute on Aging, grant ZEN-08-87090 from the Alzheimer's Association, the Lavoisier grant from the French Ministry of Foreign and European Affairs, the France-Berkeley Fund, the Thérèse et René Planiol foundation, and the Société de Neuropsychologie de Langue Francaise association., University of California [Berkeley] (UC Berkeley), University of California (UC)-University of California (UC), Eustache, Francis, Institut National de la Santé et de la Recherche Médicale ( INSERM ) -École pratique des hautes études ( EPHE ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), The Helen Wills Neuroscience Institute ( HWNI ), The Life Sciences Division, and Lawrence Berkeley National Laboratory [Berkeley] ( LBNL ) -University of California [Berkeley]
Subjects: Male, Aging, MESH : Aged, MESH: Cognition, Audiology, Neuropsychological Tests, MESH : Prefrontal Cortex, 0302 clinical medicine, Cognition, Reference Values, MESH: Aging, positron emission tomography (PET), MESH: Memory, Episodic memory, Aged, 80 and over, Cerebral Cortex, 0303 health sciences, MESH : Aniline Compounds, MESH: Middle Aged, amyloid-beta (Aβ), MESH: Reference Values, MESH: Image Processing, Computer-Assisted, MESH: Amyloid beta-Peptides, 3. Good health, normal aging, MESH : Cognition, Alzheimer's disease (AD), MESH : Cerebral Cortex, Alzheimer's disease, MESH : Image Processing, Computer-Assisted, medicine.medical_specialty, MESH : Cohort Studies, Article, MESH: Aniline Compounds, 03 medical and health sciences, Memory, MESH : Magnetic Resonance Imaging, Humans, MESH : Middle Aged, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Aged, 80 and over, Aged, MESH: Humans, MESH : Humans, MESH : Positron-Emission Tomography, medicine.disease, MESH : Aging, MESH: Cerebral Cortex, Thiazoles, MESH: Cognition Disorders, MESH : Radiopharmaceuticals, chemistry, Positron-Emission Tomography, Neurology (clinical), Radiopharmaceuticals, Pittsburgh compound B, Cognition Disorders, Neuroscience, MESH: Female, 030217 neurology & neurosurgery, Biomarkers, [11C]-Pittsburgh compound B (PiB), MESH: Magnetic Resonance Imaging, Cohort Studies, chemistry.chemical_compound, MESH: Aged, 80 and over, MESH : Thiazoles, Image Processing, Computer-Assisted, MESH : Female, Prefrontal cortex, MESH: Cohort Studies, MESH: Aged, Aniline Compounds, MESH : Memory, Neuropsychology, MESH: Neuropsychological Tests, Middle Aged, Magnetic Resonance Imaging, MESH: Positron-Emission Tomography, MESH : Neuropsychological Tests, medicine.anatomical_structure, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Psychology, MESH: Radiopharmaceuticals, MESH : Male, Prefrontal Cortex, MESH: Thiazoles, MESH : Reference Values, MESH : Amyloid beta-Peptides, MESH : Cognition Disorders, Arts and Humanities (miscellaneous), medicine, Effects of sleep deprivation on cognitive performance, Anterior cingulate cortex, 030304 developmental biology, Amyloid beta-Peptides, subjective cognition, MESH: Biological Markers, MESH: Male, MESH : Biological Markers, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Posterior cingulate, MESH: Prefrontal Cortex
Abstract: International audience; OBJECTIVE: To study the relationship between subjective cognition and the neuropathological hallmark of Alzheimer disease (AD), amyloid-β (Aβ) deposition, using carbon 11-labeled Pittsburgh Compound B (PiB) positron emission tomography in normal elderly individuals. DESIGN: Cross-sectional analysis. SUBJECTS: Forty-eight cognitively normal elderly subjects (11 with high PiB uptake and 28 with low PiB uptake) were included. All underwent clinical and neuropsychological evaluations, magnetic resonance imaging, and positron emission tomography. SETTING: Berkeley Aging Cohort Study. MAIN OUTCOME MEASURE: Relationship between PiB uptake and subjective cognition measures. RESULTS: Subjects with high PiB uptake showed significantly lower performance than those with low PiB uptake on an episodic memory measure and were less confident about their general memory abilities when required to evaluate themselves relative to other people of the same age. High and low PiB uptake groups did not differ on the accuracy of their cognitive self-reports compared with objective cognitive performance. General memory self-reports from the whole group were significantly correlated with regional PiB uptake in the right medial prefrontal cortex and anterior cingulate cortex and in the right precuneus and posterior cingulate cortex. Reduced confidence about memory abilities was associated with greater PiB uptake in these brain regions. All results were independent of demographic variables and depressive affects. CONCLUSIONS: A decrease of self-confidence about memory abilities in cognitively normal elderly subjects may be related to the neuropathological hallmark of AD measured with PiB-positron emission tomography. Subjective cognitive impairment may represent a very early clinical manifestation of AD.
Published: 2012

8. Sleep contributes to the strengthening of some memories over others, depending on hippocampal activity at learning. : Sleep, the hippocampus and memory consolidation

Author: Christine Bastin, Brigitte Landeau, Pierre Maquet, André Luxen, Fabienne Collette, Dorothée Feyers, Géraldine Rauchs, Cyclotron Research Centre, Université de Liège, Neuropsychologie cognitive et neuroanatomie fonctionnelles de la mémoire humaine, Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Cognitive and Behavioural Neuroscience Centre, This research was supported by the Belgian Fonds National de la Recherche Scientifique (FNRS), the Fondation Médicale Reine Elisabeth (FMRE), the Research Fund of the University of Liège and 'Interuniversity Attraction Poles Programme - Belgian State - Belgian Science Policy'., Université de Caen Normandie ( UNICAEN ), and Normandie Université ( NU ) -Normandie Université ( NU ) -École pratique des hautes études ( EPHE ) -Institut National de la Santé et de la Recherche Médicale ( INSERM )
Subjects: Male, MESH: Decision Making, MESH: Hippocampus, hippocampus, MESH : Aged, MESH : Recognition (Psychology), MESH: Mental Recall, MESH: Cognition, MESH : Hippocampus, Brain mapping, Memorization, MESH: Semantics, 0302 clinical medicine, MESH : Semantics, MESH: Memory, fMRI, MESH: Sleep Deprivation, MESH: Image Processing, Computer-Assisted, MESH: Recognition (Psychology), memory consolidation, MESH: Photic Stimulation, MESH: Young Adult, MESH : Cognition, MESH : Image Processing, Computer-Assisted, MESH: Oxygen, MESH: Sleep, MESH : Sleep, MESH : Young Adult, MESH: Reading, Statistics, Nonparametric, Article, 03 medical and health sciences, MESH : Magnetic Resonance Imaging, Encoding (memory), Reaction Time, Humans, MESH: Humans, MESH : Cues, MESH : Mental Recall, MESH : Humans, Recognition, Psychology, MESH: Adult, sleep deprivation, Oxygen, MESH : Reaction Time, Mental Recall, MESH : Intelligence Tests, MESH: Intelligence Tests, MESH: Female, Photic Stimulation, 030217 neurology & neurosurgery, MESH: Alzheimer Disease, MESH: Cues, MESH : Oxygen, MESH : Brain Mapping, MESH : Alzheimer Disease, MESH : Photic Stimulation, Hippocampus, MESH : Analysis of Variance, MESH: Magnetic Resonance Imaging, Image Processing, Computer-Assisted, MESH : Female, MESH : Frontotemporal Lobar Degeneration, MESH: Brain Mapping, MESH: Aged, Brain Mapping, MESH: Statistics, Nonparametric, General Neuroscience, MESH : Memory, 05 social sciences, Motivated forgetting, MESH: Neuropsychological Tests, MESH : Adult, Verbal Learning, Magnetic Resonance Imaging, MESH : Neuropsychological Tests, MESH : Verbal Learning, Female, Memory consolidation, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Sleep (system call), MESH: Frontotemporal Lobar Degeneration, medicine.symptom, Psychology, Cognitive psychology, Adult, MESH : Male, 050105 experimental psychology, directed forgetting, Young Adult, MESH: Analysis of Variance, medicine, 0501 psychology and cognitive sciences, sleep, MESH : Statistics, Nonparametric, Analysis of Variance, MESH : Sleep Deprivation, MESH : Reading, MESH: Verbal Learning, MESH : Decision Making, MESH: Male, MESH: Reaction Time, Sleep deprivation, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Abstract: International audience; Memory consolidation benefits from sleep. In addition to strengthening some memory traces, another crucial, albeit overlooked, function of memory is to erase irrelevant information. Directed forgetting is an experimental approach consisting in presenting "to be remembered" and "to be forgotten" information that allows selectively decreasing or increasing the strength of individual memory traces according to the instruction provided at learning. This paradigm was used in combination with functional MRI to determine, in humans, what specifically triggers at encoding sleep-dependent compared with time-dependent consolidation. Our data indicate that relevant items that subjects strived to memorize are consolidated during sleep to a greater extent than items that participants did not intend to learn. This process appears to depend on a differential activation of the hippocampus at encoding, which acts as a signal for the offline reprocessing of relevant memories during postlearning sleep episodes.
Published: 2011

9. Sleep contributes to the strengthening of some memories over others, depending on hippocampal activity at learning

Author: Rauchs, Géraldine, Feyers, Dorothée, Landeau, Brigitte, Bastin, Christine, Luxen, André, Maquet, Pierre, Collette, Fabienne, Cyclotron Research Centre, Université de Liège, Neuropsychologie cognitive et neuroanatomie fonctionnelles de la mémoire humaine, Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -École pratique des hautes études ( EPHE ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Cognitive and Behavioural Neuroscience Centre, This research was supported by the Belgian Fonds National de la Recherche Scientifique (FNRS), the Fondation Médicale Reine Elisabeth (FMRE), the Research Fund of the University of Liège and 'Interuniversity Attraction Poles Programme - Belgian State - Belgian Science Policy'., Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Caen Normandie (UNICAEN), and Normandie Université (NU)-Normandie Université (NU)
Subjects: MESH: Decision Making, MESH: Hippocampus, MESH : Oxygen, hippocampus, MESH : Brain Mapping, MESH : Aged, MESH : Recognition (Psychology), MESH: Mental Recall, MESH : Alzheimer Disease, MESH : Photic Stimulation, MESH: Cognition, MESH : Analysis of Variance, MESH : Hippocampus, MESH: Semantics, MESH: Magnetic Resonance Imaging, MESH : Female, MESH: Memory, MESH : Semantics, MESH : Frontotemporal Lobar Degeneration, MESH: Brain Mapping, MESH: Aged, MESH: Statistics, Nonparametric, fMRI, MESH : Memory, MESH: Neuropsychological Tests, MESH: Sleep Deprivation, MESH : Adult, MESH: Image Processing, Computer-Assisted, MESH: Recognition (Psychology), MESH : Neuropsychological Tests, MESH: Photic Stimulation, memory consolidation, MESH: Young Adult, MESH : Cognition, MESH : Verbal Learning, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Frontotemporal Lobar Degeneration, MESH: Oxygen, MESH : Image Processing, Computer-Assisted, MESH: Sleep, MESH : Sleep, MESH : Male, MESH : Young Adult, MESH: Reading, directed forgetting, MESH: Analysis of Variance, MESH : Magnetic Resonance Imaging, sleep, MESH : Statistics, Nonparametric, MESH: Humans, MESH : Cues, MESH : Sleep Deprivation, MESH : Mental Recall, MESH : Humans, MESH: Adult, MESH : Reading, MESH: Verbal Learning, sleep deprivation, MESH: Male, MESH : Decision Making, MESH: Reaction Time, MESH : Reaction Time, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Intelligence Tests, MESH: Intelligence Tests, MESH: Female, MESH: Alzheimer Disease, MESH: Cues
Abstract: International audience; Memory consolidation benefits from sleep. In addition to strengthening some memory traces, another crucial, albeit overlooked, function of memory is to erase irrelevant information. Directed forgetting is an experimental approach consisting in presenting "to be remembered" and "to be forgotten" information that allows selectively decreasing or increasing the strength of individual memory traces according to the instruction provided at learning. This paradigm was used in combination with functional MRI to determine, in humans, what specifically triggers at encoding sleep-dependent compared with time-dependent consolidation. Our data indicate that relevant items that subjects strived to memorize are consolidated during sleep to a greater extent than items that participants did not intend to learn. This process appears to depend on a differential activation of the hippocampus at encoding, which acts as a signal for the offline reprocessing of relevant memories during postlearning sleep episodes.
Published: 2011

10. Mental time travel into the past and the future in healthy aged adults: an fMRI study. : Mental time travel into the past and the future

Author: Viard, Armelle, Chételat, Gaël, Lebreton, Karine, Desgranges, Béatrice, Landeau, Brigitte, De La Sayette, Vincent, Eustache, Francis, Piolino, Pascale, Neuropsychologie cognitive et neuroanatomie fonctionnelles de la mémoire humaine, Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -École pratique des hautes études ( EPHE ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire de Psychologie et Neurosciences Cognitives ( LPNCog ), Université Paris Descartes - Paris 5 ( UPD5 ) -Centre National de la Recherche Scientifique ( CNRS ) -Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Laboratoire de Psychologie et Neurosciences Cognitives (LPNCog / UMR 8189), and Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)
Subjects: MESH: Hippocampus, hippocampus, MESH : Aged, MESH: Mental Recall, fMRI, MESH: Cognition, MESH: Frontal Lobe, MESH : Hippocampus, MESH : Prefrontal Cortex, MESH: Semantics, MESH: Magnetic Resonance Imaging, MESH : Neural Pathways, MESH: Aging, MESH : Female, MESH : Semantics, MESH : Gyrus Cinguli, MESH: Aged, MESH: Thinking, MESH: Middle Aged, autobiographical memory, MESH : Thinking, MESH: Neuropsychological Tests, episodic memory, MESH: Image Processing, Computer-Assisted, MESH : Neuropsychological Tests, MESH : Cognition, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Image Processing, Computer-Assisted, MESH: Occipital Lobe, MESH : Imagination, MESH: Brain, MESH: Gyrus Cinguli, MESH : Magnetic Resonance Imaging, MESH: Temporal Lobe, MESH : Frontal Lobe, MESH : Middle Aged, MESH: Imagination, MESH : Temporal Lobe, future thinking, MESH: Humans, MESH : Occipital Lobe, MESH: Neural Pathways, MESH : Mental Recall, MESH : Humans, MESH : Aging, MESH : Brain, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Prefrontal Cortex, MESH: Female
Abstract: International audience; Remembering the past and envisioning the future rely on episodic memory which enables mental time travel. Studies in young adults indicate that past and future thinking share common cognitive and neural underpinnings. No imaging data is yet available in healthy aged subjects. Using fMRI, we scanned older subjects while they remembered personal events (PP: last 12 months) or envisioned future plans (FP: next 12 months). Behaviorally, both time-periods were comparable in terms of visual search strategy, emotion, frequency of rehearsal and recency of the last evocation. However, PP were more episodic, engaged a higher state of autonoetic consciousness and mental visual images were clearer and more numerous than FP. Neuroimaging results revealed a common network of activation (posterior cingulate cortex, precuneus, prefrontal cortex, hippocampus) reflecting the use of similar cognitive processes. Furthermore, the episodic nature of PP depended on hippocampal and visuo-spatial activations (occipital and angular gyri), while, for FP, it depended on the inferior frontal and lateral temporal gyri, involved in semantic memory retrieval. The common neural network and behavior suggests that healthy aged subjects thought about their future prospects in the past. The contribution of retrospective thinking into the future that engages the same network as the one recruited when remembering the past is discussed. Within this network, differential recruitment of specific areas highlights the episodic distinction between past and future mental time travel.
Published: 2011

11. Mental time travel into the past and the future in healthy aged adults: an fMRI study. : Mental time travel into the past and the future

Author: Viard , Armelle, Chételat , Gaël, Lebreton , Karine, Desgranges , Béatrice, Landeau , Brigitte, De La Sayette , Vincent, Eustache , Francis, Piolino , Pascale, Neuropsychologie cognitive et neuroanatomie fonctionnelles de la mémoire humaine, Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -École pratique des hautes études ( EPHE ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire de Psychologie et Neurosciences Cognitives ( LPNCog ), and Université Paris Descartes - Paris 5 ( UPD5 ) -Centre National de la Recherche Scientifique ( CNRS ) -Centre National de la Recherche Scientifique ( CNRS )
Subjects: MESH : Imagination, hippocampus, MESH : Aged, fMRI, MESH : Hippocampus, MESH : Prefrontal Cortex, MESH : Neural Pathways, MESH : Magnetic Resonance Imaging, MESH : Frontal Lobe, MESH : Middle Aged, MESH : Female, MESH : Semantics, MESH : Gyrus Cinguli, MESH : Temporal Lobe, future thinking, MESH : Occipital Lobe, autobiographical memory, MESH : Mental Recall, MESH : Humans, MESH : Thinking, episodic memory, MESH : Aging, MESH : Neuropsychological Tests, MESH : Brain, MESH : Cognition, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Image Processing, Computer-Assisted
Abstract: International audience; Remembering the past and envisioning the future rely on episodic memory which enables mental time travel. Studies in young adults indicate that past and future thinking share common cognitive and neural underpinnings. No imaging data is yet available in healthy aged subjects. Using fMRI, we scanned older subjects while they remembered personal events (PP: last 12 months) or envisioned future plans (FP: next 12 months). Behaviorally, both time-periods were comparable in terms of visual search strategy, emotion, frequency of rehearsal and recency of the last evocation. However, PP were more episodic, engaged a higher state of autonoetic consciousness and mental visual images were clearer and more numerous than FP. Neuroimaging results revealed a common network of activation (posterior cingulate cortex, precuneus, prefrontal cortex, hippocampus) reflecting the use of similar cognitive processes. Furthermore, the episodic nature of PP depended on hippocampal and visuo-spatial activations (occipital and angular gyri), while, for FP, it depended on the inferior frontal and lateral temporal gyri, involved in semantic memory retrieval. The common neural network and behavior suggests that healthy aged subjects thought about their future prospects in the past. The contribution of retrospective thinking into the future that engages the same network as the one recruited when remembering the past is discussed. Within this network, differential recruitment of specific areas highlights the episodic distinction between past and future mental time travel.
Published: 2011

12. Validating an interlingual metanorm for emotional analysis of texts

Author: Nicolas Leveau, Ba-Linh Nguyen, Guy Denhière, Sandra Jhean-Larose, Cognitions Humaine et ARTificielle (CHART), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Paris Nanterre (UPN)-Université Paris 8 Vincennes-Saint-Denis (UP8)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Tijus, Charles, École pratique des hautes études (EPHE)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Université Paris 8 Vincennes-Saint-Denis (UP8)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Nanterre (UPN)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Education Discours Apprentissages (EDA - EA 4071), Université Paris Descartes - Paris 5 (UPD5), Laboratoire de psychologie cognitive (LPC), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Psychobiologie du Développement, École pratique des hautes études (EPHE), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Université Paris 8 Vincennes-Saint-Denis (UP8)-École Pratique des Hautes Études (EPHE), École Pratique des Hautes Études (EPHE), Cognitions Humaine et ARTificielle ( CHART ), Université Paris 8 Vincennes-Saint-Denis ( UP8 ) -École pratique des hautes études ( EPHE ) -Université Paris Nanterre ( UPN ), Education Discours Apprentissages ( EDA - EA 4071 ), Université Paris Descartes - Paris 5 ( UPD5 ), Laboratoire de psychologie cognitive ( LPC ), Centre National de la Recherche Scientifique ( CNRS ) -Aix Marseille Université ( AMU ), and École pratique des hautes études ( EPHE )
Subjects: Image Processing, Emotions, [ SCCO.PSYC ] Cognitive science/Psychology, 050109 social psychology, Correlation, German, Computer-Assisted, Image Processing, Computer-Assisted, Developmental and Educational Psychology, General Psychology, MESH : Judgment, Language, 05 social sciences, [ SHS.EDU ] Humanities and Social Sciences/Education, MESH: Image Processing, Computer-Assisted, MESH: Internet, MESH: Language, [SCCO.PSYC]Cognitive science/Psychology, language, Psychology (miscellaneous), MESH : Internet, Psychology, Arousal, Social psychology, MESH : Image Processing, Computer-Assisted, Cognitive psychology, MESH: Terminology as Topic, Emotion classification, [SHS.EDU]Humanities and Social Sciences/Education, [SHS.INFO]Humanities and Social Sciences/Library and information sciences, Experimental and Cognitive Psychology, MESH : Emotions, 050105 experimental psychology, [SHS.INFO] Humanities and Social Sciences/Library and information sciences, Judgment, Arts and Humanities (miscellaneous), MESH : Language, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, Terminology as Topic, Humans, 0501 psychology and cognitive sciences, Valence (psychology), MESH : Arousal, MESH: Emotions, MESH: Judgment, Internet, MESH: Humans, MESH: Arousal, MESH : Humans, MESH : Terminology as Topic, language.human_language, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: In this article, we present a set of 12 norms that characterize emotional terms in French, English, German, Spanish, Italian, and Finnish. The high correlation between the norm values in the two emotional dimensions of valence and arousal suggests an interlingual homogeneity of emotional representations and allows a significant metanorm-EMONORM-to be established with 6,383 terms characterized in valence and 4,345 terms characterized in arousal. This metanorm is a resource for creating experimental materials in studies on language and emotions. Furthermore, we perform three tests using EMONORM, with the objectives of (1) identifying basic emotions from their valence and arousal values, (2) determining the orientation of texts referring to positive and negative emotions, and (3) evaluating the intensity of emotions expressed in texts. The results are highly similar to those for human judgments. Finally, we present EMOVAL/SEMOTEX, a Web application for static and dynamic valence and arousal emotional analysis of texts using EMONORM ( http://www.semotex.fr ).
Published: 2011

13. Spatiotemporal motion estimation for respiratory-correlated imaging of the lungs

Author: Jef Vandemeulebroucke, Simon Rit, Jan Kybic, Patrick Clarysse, David Sarrut, Calvat, Pascal, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé ( CREATIS ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'InfoRmatique en Image et Systèmes d'information ( LIRIS ), Université Lumière - Lyon 2 ( UL2 ) -École Centrale de Lyon ( ECL ), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ) -Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ), Center for Machine Perception, Czech Technical University in Prague ( CTU ), 4 - Imagerie Tomographique et Radiothérapie, Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), CCIN2P3, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Imagerie Tomographique et Radiothérapie, Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Czech Technical University in Prague (CTU), and Electronics and Informatics
Subjects: Time Factors, respiratory motion, MESH : Movement, MESH : Four-Dimensional Computed Tomography, Movement, MESH : Lung, Diaphragm, Physics::Medical Physics, MESH: Movement, MESH : Models, Biological, Thoracic Radiotherapy, Models, Biological, MESH : Artifacts, 4D Ct, Deformable Registration, Image Processing, Computer-Assisted, Organ Motion, MESH: Lung, [ SDV.IB ] Life Sciences [q-bio]/Bioengineering, Four-Dimensional Computed Tomography, 4-Dimensional Computed-Tomography, Lung, Ct, Deformations, MESH: Respiration, [SDV.IB] Life Sciences [q-bio]/Bioengineering, MESH: Four-Dimensional Computed Tomography, Respiration, MESH: Time Factors, MESH: Models, Biological, MESH: Image Processing, Computer-Assisted, MESH : Respiration, Mr-Images, MESH: Artifacts, MESH: Diaphragm, Nonrigid Registration, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Reconstruction, MESH : Diaphragm, Artifacts, MESH : Image Processing, Computer-Assisted, Model, MESH : Time Factors
Abstract: International audience; PURPOSE: Four-dimensional computed tomography (4D CT) can provide patient-specific motion information for radiotherapy planning and delivery. Motion estimation in 4D CT is challenging due to the reduced image quality and the presence of artifacts. We aim to improve the robustness of deformable registration applied to respiratory-correlated imaging of the lungs, by using a global problem formulation and pursuing a restrictive parametrization for the spatiotemporal deformation model. METHODS: A spatial transformation based on free-form deformations was extended to the temporal domain, by explicitly modeling the trajectory using a cyclic temporal model based on B-splines. A global registration criterion allowed to consider the entire image sequence simultaneously and enforce the temporal coherence of the deformation throughout the respiratory cycle. To ensure a parametrization capable of capturing the dynamics of respiratory motion, a prestudy was performed on the temporal dimension separately. The temporal parameters were tuned by fitting them to diaphragm motion data acquired for a large patient group. Suitable properties were retained and applied to spatiotemporal registration of 4D CT data. Registration results were validated using large sets of landmarks and compared to consecutive spatial registrations. To illustrate the benefit of the spatiotemporal approach, we also assessed the performance in the presence of motion-induced artifacts. RESULTS: Cubic B-splines gave better or similar fitting results as lower orders and were selected because of their inherently stronger regularization. The fitting and registration errors increased gradually with the temporal control point spacing, representing a trade-off between achievable accuracy and sensitivity to noise and artifacts. A piecewise smooth trajectory model, allowing for a discontinuous change of speed at end-inhale, was found most suitable to account for the sudden changes of motion at this breathing phase. The spatiotemporal modeling allowed a reduction of the number of parameters of 45%, while maintaining registration accuracy within 0.1 mm. The approach reduced the sensitivity to artifacts. CONCLUSIONS: Spatiotemporal registration can provide accurate motion estimation for 4D CT and improves the robustness to artifacts.
Published: 2011

14. Decrease of prefrontal metabolism after subthalamic stimulation in obsessive-compulsive disorder: a positron emission tomography study

Author: Julie Anne Peron, Mircea Polosan, Florence Le Jeune, Denys Fontaine, Sophie Tezenas du Montcel, Marie-Odile Krebs, Antoine Pelissolo, Aurélie Bourguignon, Dominique Drapier, Nicolas Baup, Bertrand Devaux, Paul Sauleau, Bruno Millet, Sylvie Raoul, Nematollah Jaafari, Isabelle Chereau, Etienne Garin, Marc Vérin, Emmanuelle Leray, Luc Mallet, K. N’Diaye, Jérôme Yelnik, Département de médecine nucléaire [Rennes], CRLCC Eugène Marquis (CRLCC), Comportement et noyaux gris centraux = Behavior and Basal Ganglia [Rennes], Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université européenne de Bretagne - European University of Brittany (UEB)-CHU Pontchaillou [Rennes]-Institut des Neurosciences Cliniques de Rennes (INCR), Service de Neurologie [Rennes] = Neurology [Rennes], CHU Pontchaillou [Rennes], Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de psychiatrie, CHU Pontchaillou [Rennes]-Hôpital Guillaume Régnier, Service de santé publique et d'épidémiologie, Hôpital Pontchaillou-CHU Pontchaillou [Rennes], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Hôpital Sainte-Anne, CIC AP-HP (pitie-Salpetriere)/inserm, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), ANTE-INSERM U836, équipe 11, Fonctions cérébrales et neuromodulation, CHU Grenoble-CHU Grenoble, Service de Neurochirurgie, Centre Hospitalier Universitaire de Nice (CHU Nice), CHU Clermont-Ferrand, Service de physiologie, Service de neurochirurgie, CIC - Poitiers, Université de Poitiers-Centre hospitalier universitaire de Poitiers (CHU Poitiers)-Direction Générale de l'Organisation des Soins (DGOS)-Institut National de la Santé et de la Recherche Médicale (INSERM), This study was supported by a grant from Medtronic to Dr. Millet., French Stimulation dans le trouble obsessionnel compulsif (STOC) study group, Pollak, Pierre, David, Olivier, Université de Rennes (UR)-Université européenne de Bretagne - European University of Brittany (UEB)-CHU Pontchaillou [Rennes]-Institut des Neurosciences Cliniques de Rennes (INCR), Laboratoire de psychologie cognitive et sociale (LPCS), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), The French Stimulation dans le trouble obsessionnel compulsif (STOC) study group, CHU Pontchaillou [Rennes]-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université européenne de Bretagne - European University of Brittany (UEB)-Institut des Neurosciences Cliniques de Rennes (INCR), Recherche en Pharmaco-épidémiologie et Recours aux Soins (REPERES), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP), EA Management des Organisations de Santé (EA MOS), École des Hautes Études en Santé Publique [EHESP] (EHESP)-PRES Sorbonne Paris Cité, École des Hautes Études en Santé Publique [EHESP] (EHESP), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de psychiatrie adulte [CHU Pitié-Salpêtière], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de Psychiatrie, CHU Grenoble, Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5), Service de neurochirurgie [Rennes] = Neurosurgery [Rennes], Microenvironnement et remodelage, Service de médecine nucléaire [Rennes], CRLCC Eugène Marquis (CRLCC)-CRLCC Eugène Marquis (CRLCC)-Foie, métabolismes et cancer, Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre hospitalier universitaire de Nantes (CHU Nantes), Centre Hospitalier Guillaume Régnier (CHGR), CH Régnier, Université de Rennes (UR)-Université européenne de Bretagne - European University of Brittany (UEB)-CHU Pontchaillou [Rennes]-Institut des Neurosciences Cliniques de Rennes = Institute of Clinical Neurosciences of Rennes (INCR), CRLCC Eugène Marquis ( CRLCC ), Comportement et noyaux gris centraux [Rennes], Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Université européenne de Bretagne ( UEB ) -CHU Pontchaillou [Rennes]-Institut des Neurosciences Cliniques de Rennes (INCR), Service de Neurologie [Rennes], Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière ( CRICM ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Service d'informatique médicale, Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Pitié-Salpêtrière [APHP], Assistance publique - Hôpitaux de Paris (AP-HP)-Université Paris Descartes - Paris 5 ( UPD5 ) -Hôpital Sainte-Anne, Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), CHU Nice, Université de Poitiers-Centre hospitalier universitaire de Poitiers ( CHU Poitiers ) -Direction Générale de l'Organisation des Soins (DGOS)-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut des Neurosciences Cliniques de Rennes (INCR)-CHU Pontchaillou [Rennes]-Université européenne de Bretagne - European University of Brittany (UEB)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Pitié-Salpêtrière [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Descartes - Paris 5 (UPD5)-Hôpital Sainte-Anne, Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Pitié-Salpêtrière [APHP], Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-CHU Pitié-Salpêtrière [APHP], Service de neurochirurgie [Rennes], Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Subjects: Male, Obsessive-Compulsive Disorder, Deep Brain Stimulation, medicine.medical_treatment, MESH : Prefrontal Cortex, Severity of Illness Index, 0302 clinical medicine, obsessive compulsive disorder, MESH : Obsessive-Compulsive Disorder, MESH : Gyrus Cinguli, MESH: Treatment Outcome, subthalamic nucleus, MESH: Middle Aged, MESH: Image Processing, Computer-Assisted, 3. Good health, MESH: Adult Brain Mapping Cross-Over Studies Deep Brain Stimulation Double-Blind Method Female Gyrus Cinguli/radionuclide imaging Humans Image Processing, Computer-Assisted Male Middle Aged Obsessive-Compulsive Disorder/radionuclide imaging* Obsessive-Compulsive Disorder/therapy* Positron-Emission Tomography Prefrontal Cortex/radionuclide imaging* Severity of Illness Index Statistics, Nonparametric Subthalamic Nucleus/surgery* Treatment Outcome, MESH : Image Processing, Computer-Assisted, medicine.medical_specialty, Deep brain stimulation, behavioral disciplines and activities, Statistics, Nonparametric, 03 medical and health sciences, MESH: Severity of Illness Index, MESH: Gyrus Cinguli, MESH : Double-Blind Method, Humans, MESH : Middle Aged, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Biological Psychiatry, MESH: Subthalamic Nucleus, MESH: Obsessive-Compulsive Disorder, MESH: Humans, MESH : Deep Brain Stimulation, MESH : Humans, MESH : Positron-Emission Tomography, MESH: Adult, nervous system diseases, 030227 psychiatry, Subthalamic Nucleus/surgery, nervous system, Positron-Emission Tomography, MESH: Deep Brain Stimulation, orbitofrontal cortex, Gyrus Cinguli/radionuclide imaging, Neuroscience, MESH: Female, 030217 neurology & neurosurgery, Cingulate cortex, 18FDG-PET, MESH : Brain Mapping, Gyrus, Image Processing, Computer-Assisted, MESH : Female, MESH: Double-Blind Method, Prefrontal cortex, MESH: Brain Mapping, Brain Mapping, MESH: Statistics, Nonparametric, Cross-Over Studies, Putamen, MESH : Adult, Middle Aged, MESH: Positron-Emission Tomography, deep brain stimulation, Subthalamic nucleus, surgical procedures, operative, Treatment Outcome, medicine.anatomical_structure, Cingulate gyrus, Cardiology, MESH : Severity of Illness Index, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Internal capsule, Psychology, therapeutics, Adult, MESH : Male, MESH : Subthalamic Nucleus, Prefrontal Cortex, MESH : Treatment Outcome, Gyrus Cinguli, MESH: Cross-Over Studies, Double-Blind Method, Internal medicine, medicine, MESH : Statistics, Nonparametric, Prefrontal Cortex/radionuclide imaging, Postcentral gyrus, [SCCO.NEUR]Cognitive science/Neuroscience, MESH : Cross-Over Studies, MESH: Male, ddc:616.8, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Orbitofrontal cortex, MESH: Prefrontal Cortex, Obsessive-Compulsive Disorder/radionuclide imaging/therapy
Abstract: International audience; Background:High-frequency bilateral subthalamic nucleus (STN) deep brain stimulation (DBS) is a promising treatment in refractory obsessive-compulsive disorder (OCD). Method Using the crossover, randomized, and double-blind procedure adopted by the STOC study, 10 patients treated with high-frequency bilateral STN DBS underwent am 18-fluorodeoxyglucose positron emission tomography (PET) investigation to highlight the neural substratum of this therapeutic approach. Results The median YaleBrown Obsessive Compulsive Scale (Y-BOCS) scores for all 10 patients were 31 (minimum = 18, maximum = 36) with "Off-Stimulation" status and 19 (minimum = 0, maximum = 30) with "On-Stimulation" status (p = .05). The OCD patients in Off-Stimulation status showed a hypermetabolism in the right frontal middle and superior gyri, right parietal lobe, postcentral gyrus, and bilateral putamen compared with healthy control subjects. A significant decrease in cerebral metabolism was observed in the left cingulate gyrus and the left frontal medial gyrus in On-Stimulation conditions compared with Off-Stimulation conditions. In addition, the improvement assessed by Y-BOCS scores during the On-Stimulation conditions was positively correlated with PET signal changes at the boundary of the orbitofrontal cortex and the medial prefrontal cortex, between PET signal changes and the Y-BOCS scores modifications in On-Stimulation status. Conclusion This study suggests that the therapeutic effect of STN DBS is related to a decrease in prefrontal cortex metabolism. © 2010 Society of Biological Psychiatry.
Published: 2010

15. In situ protein expression in tumour spheres: development of an immunostaining protocol for confocal microscopy

Author: Bruno Saubaméa, Dominique Bellet, Virginie Dangles-Marie, Louis-Bastien Weiswald, Sophie Richon, Jean-Marc Guinebretière, Institut des sciences du Médicament -Toxicologie - Chimie - Environnement ( IFR71 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL ( ENSCP ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université Paris Descartes - Paris 5 ( UPD5 ), Service de Pathologie, Hôpital René HUGUENIN (Saint-Cloud)-INSTITUT CURIE, Service de Médecine Nucléaire, INSTITUT CURIE-Hôpital René HUGUENIN (Saint-Cloud), Neuropsychopharmacologie des addictions. Vulnérabilité et variabilité expérimentale et clinique ( NAVVEC (UM 81) ), Université Paris Diderot - Paris 7 ( UPD7 ) -Institut des sciences du Médicament -Toxicologie - Chimie - Environnement ( IFR71 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL ( ENSCP ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL ( ENSCP ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), This work was supported by Ligue Contre le Cancer, Comité Ile-de-France (Grant RS 06/75-71, RS 07/75-45), Institut National du Cancer et Cancérôpole Ile de France ('COLOMETASTEM' and 'CSC cross platform network' projects), Philippe and Denis Bloch Cancer Research Grant, Patrick Roy Translational Medicine Grant, Sally Paget-Brown Translational Research Grant, Geneviève and Jean-Paul Driot Transformative Research grant. LBW was supported by a predoctoral fellowship from the Association d'Aide à la Recherche Cancérologique de Saint-Cloud and from The Association pour la Recherche sur le Cance, Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Curie [Paris]-Hôpital René HUGUENIN (Saint-Cloud), Neuropsychopharmacologie des addictions. Vulnérabilité et variabilité expérimentale et clinique (NAVVEC - UM 81 (UMR 8206/ U705)), Université Paris Diderot - Paris 7 (UPD7)-Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), BMC, Ed., Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5), Hôpital René HUGUENIN (Saint-Cloud)-Institut Curie [Paris], Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Université Paris Diderot - Paris 7 (UPD7)
Subjects: Cancer Research, MESH : Immunohistochemistry, Cell, MESH: Flow Cytometry, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, law.invention, 0302 clinical medicine, law, Neoplasms, Image Processing, Computer-Assisted, MESH: Microscopy, Confocal, MESH: Neoplasms, AC133 Antigen, MESH: Antigens, CD, 0303 health sciences, Microscopy, Confocal, medicine.diagnostic_test, biology, MESH: Peptides, MESH : Peptides, MESH: Gene Expression Regulation, Neoplastic, Flow Cytometry, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Immunohistochemistry, MESH: Image Processing, Computer-Assisted, 3. Good health, Cell biology, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Technical Advance, Oncology, 030220 oncology & carcinogenesis, Neoplastic Stem Cells, MESH : Image Processing, Computer-Assisted, MESH: Cell Line, Tumor, MESH : Flow Cytometry, MESH : Gene Expression Regulation, Neoplastic, Confocal, MESH: Glycoproteins, [SDV.CAN]Life Sciences [q-bio]/Cancer, lcsh:RC254-282, Flow cytometry, MESH: Gene Expression Profiling, 03 medical and health sciences, MESH : Neoplastic Stem Cells, [SDV.CAN] Life Sciences [q-bio]/Cancer, Antigens, CD, Cancer stem cell, Confocal microscopy, Cell Line, Tumor, MESH : Antigens, CD, Genetics, medicine, Humans, MESH : Microscopy, Confocal, Glycoproteins, 030304 developmental biology, MESH: Humans, MESH : Cell Line, Tumor, Gene Expression Profiling, MESH : Gene Expression Profiling, MESH : Humans, CD44, MESH: Immunohistochemistry, MESH: Neoplastic Stem Cells, MESH : Neoplasms, MESH : Glycoproteins, Molecular biology, Cell culture, biology.protein, Peptides, Immunostaining
Abstract: Background Multicellular tumour sphere models have been shown to closely mimic phenotype characteristics of in vivo solid tumours, or to allow in vitro propagation of cancer stem cells (CSCs). CSCs are usually characterized by the expression of specific membrane markers using flow cytometry (FC) after enzymatic dissociation. Consequently, the spatial location of positive cells within spheres is not documented. Confocal microscopy is the best technique for the imaging of thick biological specimens after multi-labelling but suffers from poor antibody penetration. Thus, we describe here a new protocol for in situ confocal imaging of protein expression in intact spheroids. Methods Protein expression in whole spheroids (150 μm in diameter) from two human colon cancer cell lines, HT29 and CT320X6, has been investigated with confocal immunostaining, then compared with profiles obtained through paraffin immunohistochemistry (pIHC) and FC. Target antigens, relevant for colon cancer and with different expression patterns, have been studied. Results We first demonstrate that our procedure overcomes the well-known problem of antibody penetration in compact structures by performing immunostaining of EpCAM, a membrane protein expressed by all cells within our spheroids. EpCAM expression is detected in all cells, even the deepest ones. Likewise, antibody access is confirmed with CK20 and CD44 immunostaining. Confocal imaging shows that 100% of cells express β-catenin, mainly present in the plasma membrane with also cytoplasmic and nuclear staining, in agreement with FC and pIHC data. pIHC and confocal imaging show similar CA 19-9 cytoplasmic and membranar expression profile in a cell subpopulation. CA 19-9+ cell count confirms confocal imaging as a highly sensitive method (75%, 62% and 51%, for FC, confocal imaging and pIHC, respectively). Finally, confocal imaging reveals that the weak expression of CD133, a putative colon CSC marker, is restricted to the luminal cell surface of colorectal cancer acini, with CD133+ cellular debris into glandular lumina. Conclusion The present protocol enables in situ visualization of protein expression in compact three-dimensional models by whole mount confocal imaging, allowing the accurate localization and quantification of cells expressing specific markers. It should prove useful to study rare events like CSCs within tumour spheres.
Published: 2010

16. Vestibular asymmetry as the cause of idiopathic scoliosis: a possible answer from Xenopus

Author: Lambert, François, Malinvaud, David, Glaunès, Joan, Bergot, Catherine, Straka, Hans, Vidal, Pierre-Paul, Glaunes, J., Laboratoire de Neurobiologie des Réseaux Sensorimoteurs ( LNRS ), Université Paris Diderot - Paris 7 ( UPD7 ) -Université Paris Descartes - Paris 5 ( UPD5 ), Mathématiques Appliquées à Paris 5 ( MAP5 - UMR 8145 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National des Sciences Mathématiques et de leurs Interactions-Centre National de la Recherche Scientifique ( CNRS ), Institut de Neurosciences cognitives et intégratives d'Aquitaine (INCIA), Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1-SFR Bordeaux Neurosciences-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Neurobiologie des Réseaux Sensorimoteurs (LNRS), Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5), Mathématiques Appliquées Paris 5 (MAP5 - UMR 8145), Université Paris Descartes - Paris 5 (UPD5)-Institut National des Sciences Mathématiques et de leurs Interactions (INSMI)-Centre National de la Recherche Scientifique (CNRS), Bioingénierie et Bioimagerie Ostéo-articulaires, Biomécanique et Biomatériaux Ostéo-Articulaires (B2OA (UMR_7052)), and Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)
Subjects: [SDV]Life Sciences [q-bio], [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Xenopus, Functional Laterality, Xenopus laevis, 0302 clinical medicine, MESH : Scoliosis, Image Processing, Computer-Assisted, MESH : Xenopus laevis, MESH: Animals, MESH: Radiography, Vestibular system, 030222 orthopedics, General Neuroscience, MESH: Scoliosis, MESH: Posture, Anatomy, Articles, Skeleton (computer programming), MESH: Image Processing, Computer-Assisted, medicine.anatomical_structure, Scoliosis, Vestibular Diseases, Larva, [ SDV.NEU.NB ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, MESH: Vestibular Diseases, MESH : Image Processing, Computer-Assisted, Posture, Biology, Cartilaginous skeleton, 03 medical and health sciences, MESH: Xenopus laevis, medicine, Animals, MESH: Functional Laterality, MESH : Functional Laterality, Proprioception, MESH : Larva, MESH : Posture, MESH : Ear, Inner, medicine.disease, biology.organism_classification, Gaze, MESH : Disease Models, Animal, Sagittal plane, Radiography, Disease Models, Animal, MESH : Vestibular Diseases, Ear, Inner, MESH : Animals, MESH: Disease Models, Animal, Neuroscience, MESH: Larva, 030217 neurology & neurosurgery, MESH: Ear, Inner
Abstract: Human idiopathic scoliosis is characterized by severe deformations of the spine and skeleton. The occurrence of vestibular-related deficits in these patients is well established but it is unclear whether a vestibular pathology is the common cause for the scoliotic syndrome and the gaze/posture deficits or if the latter behavioral deficits are a consequence of the scoliotic deformations. A possible vestibular origin was tested in the frogXenopus laevisby unilateral removal of the labyrinthine endorgans at larval stages. After metamorphosis into young adult frogs, X-ray images and three-dimensional reconstructed micro-computer tomographic scans of the skeleton showed deformations similar to those of scoliotic patients. The skeletal distortions consisted of a curvature of the spine in the frontal and sagittal plane, a transverse rotation along the body axis and substantial deformations of all vertebrae. In terrestrial vertebrates, the initial postural syndrome after unilateral labyrinthectomy recovers over time and requires body weight-supporting limb proprioceptive information. In an aquatic environment, however, this information is absent. Hence, the lesion-induced asymmetric activity in descending spinal pathways and the resulting asymmetric muscular tonus persists. As a consequence the mostly cartilaginous skeleton of the frog tadpoles progressively deforms. Lack of limb proprioceptive signals in an aquatic environment is thus the element, which links theXenopusmodel with human scoliosis because a comparable situation occurs during gestationin utero. A permanently imbalanced activity in descending locomotor/posture control pathways might be the common origin for the observed structural and behavioral deficits in humans as in the different animal models of scoliosis.
Published: 2009

17. Quantification of liver fat content: comparison of triple-echo chemical shift gradient-echo imaging and in vivo proton MR spectroscopy

Author: David Masson, Denis Krausé, Jean-Michel Petit, Romaric Loffroy, Patrick Hillon, Douraied Ben Salem, Jean-Pierre Cercueil, Boris Guiu, Serge Aho, Service de radiologie et d'Imagerie médicale diagnostique et thérapeutique (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Unité de Génétique Moléculaire Animale ( UGMA ), Université de Limoges ( UNILIM ) -Institut National de la Recherche Agronomique ( INRA ), Groupe d'Etude de la Thrombose de Bretagne Occidentale ( GETBO ), Université de Brest ( UBO ), Département de radiologie [Brest] ( DR - Brest ), Centre Hospitalier Régional Universitaire de Brest ( CHRU Brest ), Service d'Ophtalmologie (CHU de Dijon), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Service d'Hépato-Gastroentérologie, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ) -Hôpital du Bocage, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Unité de Génétique Moléculaire Animale (UGMA), Université de Limoges (UNILIM)-Institut National de la Recherche Agronomique (INRA), Groupe d'Etude de la Thrombose de Bretagne Occidentale (GETBO), Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), Département de radiologie [Brest] (DR - Brest), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Hôpital du Bocage, Unité de Génétique Moléculaire Animale (UMR GMA), Université de Brest (UBO)-Université de Brest (UBO), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Service d'hépato-gastroentérologie et cancérologie digestive (CHU de Dijon)
Subjects: Male, Magnetic Resonance Spectroscopy, MESH: Echo-Planar Imaging, MESH : Fatty Liver, [SDV]Life Sciences [q-bio], MESH : Aged, MESH : Prospective Studies, MESH : Mathematical Computing, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, 0302 clinical medicine, Nuclear magnetic resonance, MESH: Aged, 80 and over, MESH : Diabetes Mellitus, Type 2, Image Processing, Computer-Assisted, Medicine, MESH : Female, Prospective Studies, MESH: Mathematical Computing, MESH: Fatty Liver, Mathematical Computing, MESH: Aged, Aged, 80 and over, MESH: Middle Aged, medicine.diagnostic_test, Echo-Planar Imaging, Echo (computing), Middle Aged, MESH: Image Processing, Computer-Assisted, Magnetic Resonance Imaging, Liver, Content (measure theory), 030211 gastroenterology & hepatology, Female, MESH : Software, MESH: Image Enhancement, MESH : Sensitivity and Specificity, MESH : Image Processing, Computer-Assisted, MESH: Diabetes Mellitus, Type 2, MESH : Male, Sensitivity and Specificity, 03 medical and health sciences, MESH: Software, Flip angle, In vivo, MESH : Magnetic Resonance Imaging, Liver fat, Humans, Radiology, Nuclear Medicine and imaging, MESH : Middle Aged, Spectroscopy, MESH : Aged, 80 and over, Aged, MESH: Humans, MESH : Echo-Planar Imaging, [ SDV ] Life Sciences [q-bio], business.industry, MESH: Magnetic Resonance Spectroscopy, MESH : Humans, MESH : Liver, Magnetic resonance imaging, Image Enhancement, Proton mr spectroscopy, MESH: Male, MESH: Prospective Studies, MESH: Sensitivity and Specificity, Fatty Liver, Diabetes Mellitus, Type 2, MESH : Magnetic Resonance Spectroscopy, MESH : Image Enhancement, business, Nuclear medicine, MESH: Female, Software, MESH: Liver
Abstract: International audience; PURPOSE: To validate a triple-echo gradient-echo sequence for measuring the fat content of the liver, by using hydrogen 1((1)H) magnetic resonance (MR) spectroscopy as the reference standard. MATERIALS AND METHODS: This prospective study was approved by the appropriate ethics committee, and written informed consent was obtained from all patients. In 37 patients with type 2 diabetes (31 men, six women; mean age, 56 years), 3.0-T single-voxel point-resolved (1)H MR spectroscopy of the liver (Couinaud segment VII) was performed to calculate the liver fat fraction from the water (4.7 ppm) and methylene (1.3 ppm) peaks, corrected for T1 and T2 decay. Liver fat fraction was also computed from triple-echo (consecutive in-phase, opposed-phase, and in-phase echo times) breath-hold spoiled gradient-echo sequence (flip angle, 20 degrees), by estimating T2* and relative signal intensity loss between in- and opposed-phase values, corrected for T2* decay. Pearson correlation coefficient, Bland-Altman 95% limit of agreement, and Lin concordance coefficient were calculated. RESULTS: Mean fat fractions calculated from the triple-echo sequence and (1)H MR spectroscopy were 10% (range, 0.7%-35.6%) and 9.7% (range, 0.2%-34.1%), respectively. Mean T2* time was 14.7 msec (range, 5.4-25.4 msec). Pearson correlation coefficient was 0.989 (P < .0001) and Lin concordance coefficient was 0.988 (P < .0001). With the Bland-Altman method, all data points were within the limits of agreement. CONCLUSION: A breath-hold triple-echo gradient-echo sequence with a low flip angle and correction for T2* decay is accurate for quantifying fat in segment VII of the liver. Given its excellent correlation and concordance with (1)H MR spectroscopy, this triple-echo sequence could replace (1)H MR spectroscopy in longitudinal studies.
Published: 2009

18. [Radiochemotherapy for oesophageal cancer: a locoregional failure history]

Author: Créhange, G., Maingon, P., Bosset, Jean-François, Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC ( CEF2P / CARCINO ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ), Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC (EA 3181) (CEF2P / CARCINO), Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)
Subjects: MESH: Combined Modality Therapy, Esophageal Neoplasms, MESH: Survival Rate, MESH: Treatment Failure, MESH : Survivors, [SDV.CAN]Life Sciences [q-bio]/Cancer, Adenocarcinoma, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, MESH : Adenocarcinoma, MESH : Treatment Failure, Image Processing, Computer-Assisted, Humans, Survivors, Treatment Failure, MESH : Carcinoma, Squamous Cell, MESH: Radiotherapy Dosage, MESH: Survivors, MESH: Humans, MESH : Radiotherapy Dosage, MESH: Adenocarcinoma, MESH : Humans, Radiotherapy Dosage, MESH: Carcinoma, Squamous Cell, MESH : Survival Rate, Combined Modality Therapy, MESH: Image Processing, Computer-Assisted, Survival Rate, MESH: Esophageal Neoplasms, Carcinoma, Squamous Cell, MESH : Esophageal Neoplasms, MESH : Combined Modality Therapy, MESH : Image Processing, Computer-Assisted
Abstract: International audience; Esophageal cancer is characterized by various degrees of lymph node invasion and metastasis, both of which are associated with a poor prognosis. Exclusive concomitant radiochemotherapy (RCT) at a dose of 50 Gy delivered over 25 sessions, according to the RTOG 85-01 protocol, has led to improved five-year survival in 25% of patients, whereas no patients survive for five years using radiotherapy alone. Surgery, even when combined with preoperative RCT, also gives disappointing results for locally advanced tumors, which casts serious doubts on the usefulness of preoperative radiotherapy. By varying the fractionation schedule, the length of treatment or the radiotherapy volumes, it has become possible to obtain levels of locoregional relapse of around 35 to 45%. The increasing incidence of adenocarcinoma, which differs from epidermoid cancer with regard to the degree of lymph node invasion, has revived discussion on radiotherapy volumes. Given this difference between these two histological forms, we propose here a number of recommendations concerning radiotherapy volumes for patients presenting with cancer of the esophagus. Finally, analysis of the results for locoregional relapse according to the dose of radiation and the recommended radiotherapy volumes, has led us to investigate why increasing the dose of radiation has no impact in esophageal cancers.
Published: 2008

19. Listeria monocytogenes EGD-e biofilms: no mushrooms but a network of knitted chains

Author: Aurélie Rieu, Romain Briandet, Olivier Habimana, Jean Guzzo, Dominique Garmyn, Pascal Piveteau, Laboratoire de Recherche en Vigne et Vin (REVV), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Bioadhésion et Hygiène des Matériaux (BHM), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Microbiologie du Sol et de l'Environnement (MSE), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB), Laboratoire de Recherche en Vigne et Vin ( REVV ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Bioadhésion et Hygiène des Matériaux ( BHM ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Microbiologie du Sol et de l'Environnement ( MSE ), and Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB )
Subjects: Image Processing, MESH : Analysis of Variance, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH : Green Fluorescent Proteins, medicine.disease_cause, MESH: Listeria monocytogenes, Applied Microbiology and Biotechnology, Bacterial Adhesion, law.invention, Green fluorescent protein, Plasmid, Computer-Assisted, law, Genes, Reporter, Image Processing, Computer-Assisted, MESH : Bacterial Proteins, MESH: Microscopy, Confocal, Pathogen, MESH: Bacterial Proteins, 2. Zero hunger, 0303 health sciences, Microscopy, Microscopy, Confocal, Photobleaching, Ecology, biology, MESH: Kinetics, MESH : Genes, Reporter, MESH: Image Processing, Computer-Assisted, MESH : Biofilms, Confocal, MESH : Kinetics, MESH: Photobleaching, MESH : Image Processing, Computer-Assisted, Biotechnology, Plasmids, MESH : Bacterial Adhesion, Green Fluorescent Proteins, MESH: Biofilms, MESH : Photobleaching, Microbiology, 03 medical and health sciences, MESH: Gene Expression Profiling, MESH: Green Fluorescent Proteins, Listeria monocytogenes, Bacterial Proteins, Confocal microscopy, MESH: Plasmids, MESH: Analysis of Variance, medicine, MESH: Bacterial Adhesion, MESH : Microscopy, Confocal, Reporter, 030304 developmental biology, Analysis of Variance, 030306 microbiology, MESH : Gene Expression Profiling, Gene Expression Profiling, MESH: Genes, Reporter, Biofilm, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Culture Media, Kinetics, Genes, MESH : Plasmids, Biofilms, MESH: Culture Media, Food Microbiology, MESH : Culture Media, MESH : Listeria monocytogenes, Bacteria, Food Science
Abstract: Listeria monocytogenes is a food pathogen that can attach on most of the surfaces encountered in the food industry. Biofilms are three-dimensional microbial structures that facilitate the persistence of pathogens on surfaces, their resistance toward antimicrobials, and the final contamination of processed goods. So far, little is known about the structural dynamics of L. monocytogenes biofilm formation and its regulation. The aims of this study were, by combining genetics and time-lapse laser-scanning confocal microscopy (LSCM), (i) to characterize the structural dynamics of L. monocytogenes EGD-e sessile growth in two nutritional environments (with or without a nutrient flow), and (ii) to evaluate the possible role of the L. monocytogenes agr system during biofilm formation by tracking the spatiotemporal fluorescence expression of a green fluorescent protein (GFP) reporter system. In the absence of nutrient flow (static conditions), unstructured biofilms composed of a few layers of cells that covered the substratum were observed. In contrast, when grown under dynamic conditions, L. monocytogenes EGD-e biofilms were highly organized. Indeed, ball-shaped microcolonies were surrounded by a network of knitted chains. The spatiotemporal tracking of fluorescence emitted by the GFP reporter system revealed that agr expression was barely detectable under static conditions, but it progressively increased during 40 h under dynamic conditions. Moreover, spatial analysis revealed that agr was expressed preferentially in cells located outside the microcolonies. Finally, the in-frame deletion of agrA , which encodes a transcriptional regulator, resulted in a decrease in initial adherence without affecting the subsequent biofilm development.
Published: 2008

20. Uncinate fasciculus fiber tracking in mesial temporal lobe epilepsy. Initial findings

Author: D. Le Bihan, Catherine Oppenheim, J.-F. Mangin, Y. Cointepas, F. Semah, Sebastian Rodrigo, C. Poupon, Narly Golestani, Francine Chassoux, Jean-François Meder, service de neuroradiologie [Paris], Hôpital Sainte-Anne, Commissariat à l'Energie Atomique, Direction des Sciences du Vivant, Département de Recherche Médicale, Service Hospitalier Frédéric Joliot, Service de Neurochirurgie, Hôpital Saint-Anne, Institut d'imagerie neurofonctionnelle ( IIN ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -ENST-Assistance publique - Hôpitaux de Paris (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -École des hautes études en sciences sociales ( EHESS ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris-Sud - Paris 11 ( UP11 ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Traitement et Communication de l'Information ( LTCI ), Télécom ParisTech-Institut Mines-Télécom [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'Imagerie et de Spectroscopie ( LRMN ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), Laboratoire de Neuroimagerie Assistée par Ordinateur ( LNAO ), Service Hospitalier Frédéric Joliot ( SHFJ ), Direction de Recherche Fondamentale (CEA) ( DRF (CEA) ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay, Center for Neuroscience and Behavioral Medicine, Children's National Medical Center, Human Brain Research Center [Kyoto] ( HBRC ), Kyoto University [Kyoto], Service NEUROSPIN ( NEUROSPIN ), National Institutes of Health [Bethesda] ( NIH ), IFR de Neuroimagerie Fonctionnelle ( IFR 49 ), DIMF, Université Paris Descartes - Paris 5 ( UPD5 ) -Centre Hospitalier Sainte-Anne, Service de neuroradiologie [Paris], Service Hospitalier Frédéric Joliot (SHFJ), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut d'imagerie neurofonctionnelle (IIN), Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École des hautes études en sciences sociales (EHESS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Ecole Nationale Supérieure des Télécommunications (ENST)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Traitement et Communication de l'Information (LTCI), Télécom ParisTech-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Imagerie et de Spectroscopie (LRMN), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire de Neuroimagerie Assistée par Ordinateur (LNAO), Human Brain Research Center [Kyoto] (HBRC), Kyoto University, Service NEUROSPIN (NEUROSPIN), National Institutes of Health [Bethesda] (NIH), IFR de Neuroimagerie Fonctionnelle (IFR 49), Université Paris Descartes - Paris 5 (UPD5)-Centre Hospitalier Sainte-Anne, Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure des Télécommunications (ENST)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École des hautes études en sciences sociales (EHESS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Santé et de la Recherche Médicale (INSERM)-ENST-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École des hautes études en sciences sociales (EHESS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Male, MESH: Hippocampus, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, MESH: Frontal Lobe, MESH : Hippocampus, Hippocampus, Nerve Fibers, Myelinated, 030218 nuclear medicine & medical imaging, 0302 clinical medicine, MESH : Neural Pathways, Reference Values, Neural Pathways, Image Processing, Computer-Assisted, MESH : Anisotropy, MESH : Female, [ SDV.IB.IMA ] Life Sciences [q-bio]/Bioengineering/Imaging, MESH: Energy Metabolism, MESH: Reference Values, General Medicine, Human brain, Anatomy, MESH : Adult, MESH : Diffusion Magnetic Resonance Imaging, MESH: Image Processing, Computer-Assisted, Temporal Lobe, Frontal Lobe, MESH: Nerve Fibers, Myelinated, medicine.anatomical_structure, MESH: Epilepsy, Temporal Lobe, MESH : Imaging, Three-Dimensional, Female, Radiology, MESH : Image Processing, Computer-Assisted, Adult, medicine.medical_specialty, Adolescent, MESH : Male, Uncinate fasciculus, MESH: Imaging, Three-Dimensional, MESH: Diffusion Magnetic Resonance Imaging, MESH : Dominance, Cerebral, Insular cortex, MESH : Reference Values, behavioral disciplines and activities, Temporal lobe, White matter, 03 medical and health sciences, Imaging, Three-Dimensional, MESH : Adolescent, MESH : Nerve Fibers, Myelinated, Fractional anisotropy, medicine, Humans, MESH: Temporal Lobe, MESH : Frontal Lobe, Radiology, Nuclear Medicine and imaging, Ictal, Dominance, Cerebral, MESH : Temporal Lobe, MESH: Adolescent, Hippocampal sclerosis, Sclerosis, MESH: Humans, business.industry, MESH: Neural Pathways, MESH : Humans, MESH: Adult, MESH: Dominance, Cerebral, medicine.disease, MESH: Male, MESH : Sclerosis, MESH : Energy Metabolism, MESH : Epilepsy, Temporal Lobe, Diffusion Magnetic Resonance Imaging, Epilepsy, Temporal Lobe, nervous system, MESH: Anisotropy, Anisotropy, MESH: Sclerosis, business, Energy Metabolism, MESH: Female, 030217 neurology & neurosurgery
Abstract: International audience; In temporal lobe epilepsy (TLE) due to hippocampal sclerosis (HS), ictal discharge spread to the frontal and insulo-perisylvian cortex is commonly observed. The implication of white matter pathways in this propagation has not been investigated. We compared diffusion tensor imaging (DTI) measurements along the uncinate fasciculus (UF), a major tract connecting the frontal and temporal lobes, in patients and controls. Ten right-handed patients referred for intractable TLE due to a right HS were investigated on a 1.5-T MR scanner including a DTI sequence. All patients had interictal fluorodeoxyglucose PET showing an ipsilateral temporal hypometabolism associated with insular and frontal or perisylvian hypometabolism. The controls consisted of ten right-handed healthy subjects. UF fiber tracking was performed, and its fractional anisotropy (FA) values were compared between patients and controls, separately for the right and left UF. The left-minus-right FA UF asymmetry index was computed to test for intergroup differences. Asymmetries were found in the control group with right-greater-than-left FA. This asymmetrical pattern was lost in the patient group. Right FA values were lower in patients with right HS versus controls. Although preliminary, these findings may be related to the preferential pathway of seizure spread from the mesial temporal lobe to frontal and insulo-perisylvian areas.
Published: 2007

21. An 11-year-old boy with drug-resistant schizophrenia treated with temporo-parietal rTMS

Author: Jean-Louis Goeb, Yvonne Delevoye-Turrell, Christine Delmaire, Bernard Lucas, Renaud Jardri, Pierre Thomas, Pierre Delion, Laboratoire de Neurosciences Fonctionnelles et Pathologies (LNFP), Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Institut Cochin (UMR_S567 / UMR 8104), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Groupe d'étude de l'atmosphère météorologique (CNRM-GAME), Institut national des sciences de l'Univers (INSU - CNRS)-Météo France-Centre National de la Recherche Scientifique (CNRS), Laboratoire des collisions atomiques et moléculaires (LCAM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Neuro-anatomie fonctionnelle du comportement et de ses troubles, Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR70-Université Pierre et Marie Curie - Paris 6 (UPMC), Laboratoire de Neurosciences Fonctionnelles et Pathologies ( LNFP ), Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique ( CNRS ), Institut Cochin ( UMR_S567 / UMR 8104 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Groupe d'étude de l'atmosphère météorologique ( CNRM-GAME ), Institut national des sciences de l'Univers ( INSU - CNRS ) -Météo France-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire des collisions atomiques et moléculaires ( LCAM ), Université Paris-Sud - Paris 11 ( UP11 ) -Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IFR70-Université Pierre et Marie Curie - Paris 6 ( UPMC ), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre national de recherches météorologiques (CNRM), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), and Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR70-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: medicine.medical_specialty, MESH : Oxygen, medicine.medical_treatment, MESH : Male, Audiology, Auditory cortex, behavioral disciplines and activities, MESH: Magnetic Resonance Imaging, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Delusion, MESH : Child, Rating scale, MESH : Schizophrenia, MESH : Magnetic Resonance Imaging, MESH: Child, mental disorders, medicine, Pervasive developmental disorder, MESH: Temporal Lobe, Antipsychotic, Psychiatry, Molecular Biology, MESH : Temporal Lobe, ComputingMilieux_MISCELLANEOUS, Auditory hallucination, MESH: Parietal Lobe, MESH: Humans, MESH : Humans, medicine.disease, MESH: Image Processing, Computer-Assisted, MESH : Transcranial Magnetic Stimulation, MESH: Male, MESH: Schizophrenia, 3. Good health, 030227 psychiatry, Transcranial magnetic stimulation, Psychiatry and Mental health, Schizophrenia, MESH: Transcranial Magnetic Stimulation, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Parietal Lobe, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], medicine.symptom, Psychology, 030217 neurology & neurosurgery, MESH : Image Processing, Computer-Assisted, MESH: Oxygen
Abstract: Very early onset schizophrenia is a psychiatric syndrome in children under 13 years of age that is characterized by a very poor prognosis, due to frequent resistance to treatment. We report here the case of a hospitalized 11-year-old child (YP), with a positive diagnosis of very early onset schizophrenia for the past 2 years (positive K-SADS-DSM-IV), without any previous sign of pervasive developmental disorder. YP experienced verbal auditory hallucinations, a delusion of alien control and heteroaggressive behaviour. Antipsychotic drugs were unsuccessful and furthermore, provoked severe acute dystonia. A 72-h EEG recording, an anatomical MRI brain scan and a complete set of metabolic analyses, were all negative. However, using data-driven analysis, we observed that YP’s functional MRI scan revealed bilateral neural activity in the auditory cortex during auditory verbal hallucinations (Figure 1). Ten sessions of fMRI-guided, low frequency (1 Hz), repeated trans-cranial magnetic stimulation (rTMS) over the left temporo-parietal cortex, were successful in stopping the verbal auditory hallucinations (Auditory Hallucination Rating Scale 47%). The improvement obtained with rTMS was maintained by repeating the sessions every 5 weeks. The clinical improvement was confirmed by a significant improvement of adaptative functions (Children’s Global Assessment Scale þ40%), and the fact that YP was able to go back home and attend school. Thereby, YP was able to receive education about his illness and to follow regular psychotherapeutic sessions. rTMS has been used for hallucinations in adults, and in children with attentional deficits and hyperactivity. To our knowledge, however, this is the first published case demonstrating the efficacy of fMRI-guided rTMS in the treatment of verbal auditory hallucinations in a child with schizophrenia. These results require replication.
Published: 2007

22. Measuring trunk orientation with a CMOS camera: feasibility and accuracy

Author: Anne-Sophie Gissot, Michel Paindavoine, G. Barbieri, Dominic Pérennou, M. Iacobelis, Laboratoire d'Etude de l'Apprentissage et du Développement [Dijon] (LEAD), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), CHU Grenoble, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Laboratoire d'Etude de l'Apprentissage et du Développement [Dijon] ( LEAD ), Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), and Université Joseph Fourier - Grenoble 1 ( UJF ) -CHU Grenoble
Subjects: 030506 rehabilitation, MESH : Stroke, Computer science, MESH: Videotape Recording, MESH : Thorax, Signal, [ SDV.NEU.SC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, 0302 clinical medicine, Image Processing, Computer-Assisted, Orthopedics and Sports Medicine, Computer vision, MESH : Algorithms, CMOS sensor, Orientation (computer vision), Noise (signal processing), Rehabilitation, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, MESH: Posture, Thorax, MESH: Image Processing, Computer-Assisted, MESH: Reproducibility of Results, Stroke, MESH : Software, 0305 other medical science, MESH : Image Processing, Computer-Assisted, Algorithms, MESH : Feasibility Studies, Posture, Biophysics, ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION, Image processing, MESH: Algorithms, MESH: Stroke, MESH: Thorax, 03 medical and health sciences, MESH: Software, Distortion, Humans, Simulation, MESH : Videotape Recording, MESH: Humans, business.industry, MESH : Reproducibility of Results, MESH : Humans, MESH : Posture, Reproducibility of Results, Videotape Recording, Trunk, Feasibility Studies, Artificial intelligence, business, Parallax, MESH: Feasibility Studies, 030217 neurology & neurosurgery, Software
Abstract: International audience; The purpose of this study was to develop and validate a new tool to objectively quantify trunk orientation at the bedside, especially dedicated to the measurement of the lateropulsion in acute and subacute stroke patients. We developed software to analyze 2D movement with a CMOS camera (Logitech Quickcam Pro 4000) and to calculate the orientation of a segment defined by two color markers. First, the accuracy, reproducibility and noise when measuring segment orientations were evaluated with the CMOS camera placed in different positions, and second trunk orientation was measured in static and in dynamic conditions both with a CMOS camera and with a gold standard 3D video system (BTS SMART-e). Results showed that the measurement was accurate (mean error=0.05+/-0.12 degrees), reproducible (S.D. over five measurements=0.005 degrees ) and steady (noise signal=0.02 degrees ). The data obtained with the CMOS camera were highly correlated with those obtained with the 3D video system both in static and in dynamic conditions. However, the CMOS camera must be relatively well centered on the measured segment to avoid error due to image distortion. The parallax error was negligible. In conclusion, this could be an important step in the postural assessment of acute and subacute stroke patients. The CMOS camera, a simple, portable, compact, low-cost, commercially available apparatus is the first tool to objectively quantify lateropulsion at the bedside. This method could also support the development of a rehabilitation program for trunk orientation based on biofeedback using the real-time signal provided by the device.
Published: 2006

23. fMRI study of problem-solving after severe traumatic brain injury

Author: Mélanie Pélégrini-Issac, Antoine Feydy, Romain Valabregue, Laurent Pierot, Fabienne Cazalis, Philippe Azouvi, Plasticité cérébrale et adaptations des fonctions visuelles et motrices, Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire d'Imagerie Fonctionnelle ( LIF ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut d'imagerie neurofonctionnelle ( IIN ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -ENST-Assistance publique - Hôpitaux de Paris (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -École des hautes études en sciences sociales ( EHESS ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris-Sud - Paris 11 ( UP11 ) -Centre National de la Recherche Scientifique ( CNRS ), Université de Reims Champagne-Ardenne ( URCA ), Service de réeducation fonctionnelle [Raymond Poincaré], Assistance publique - Hôpitaux de Paris (AP-HP)-AP-HP Hôpital Raymond Poincaré [Garches], Physiologie et physiopathologie de la motricité chez l'homme, Université Pierre et Marie Curie - Paris 6 ( UPMC ) -IFR70-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Imagerie Fonctionnelle (LIF), Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut d'imagerie neurofonctionnelle (IIN), Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure des Télécommunications (ENST)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École des hautes études en sciences sociales (EHESS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Raymond Poincaré [AP-HP], Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR70-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École des hautes études en sciences sociales (EHESS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Ecole Nationale Supérieure des Télécommunications (ENST)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Male, MESH : Brain Mapping, Neuropsychological Tests, Audiology, MESH : Prefrontal Cortex, MESH: Magnetic Resonance Imaging, 0302 clinical medicine, Cortex (anatomy), Image Processing, Computer-Assisted, Developmental and Educational Psychology, MESH : Female, MESH : Gyrus Cinguli, Problem Solving, MESH: Brain Mapping, Dysexecutive syndrome, Brain Mapping, MESH : Brain Injuries, 05 social sciences, Healthy subjects, MESH: Neuropsychological Tests, MESH : Adult, Magnetic Resonance Imaging, MESH: Case-Control Studies, MESH: Image Processing, Computer-Assisted, MESH : Neuropsychological Tests, medicine.anatomical_structure, Female, Psychology, psychological phenomena and processes, MESH : Image Processing, Computer-Assisted, MESH: Problem Solving, Adult, Brain activation, medicine.medical_specialty, MESH : Case-Control Studies, Adolescent, Traumatic brain injury, MESH : Male, Neuroscience (miscellaneous), Prefrontal Cortex, Gyrus Cinguli, behavioral disciplines and activities, 050105 experimental psychology, 03 medical and health sciences, MESH : Cognition Disorders, MESH: Gyrus Cinguli, MESH : Adolescent, MESH : Magnetic Resonance Imaging, MESH : Problem Solving, Reaction Time, medicine, Humans, 0501 psychology and cognitive sciences, Anterior cingulate cortex, MESH: Adolescent, MESH: Humans, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, MESH : Humans, MESH: Adult, medicine.disease, MESH: Male, MESH: Reaction Time, MESH: Cognition Disorders, nervous system, Brain Injuries, Case-Control Studies, MESH : Reaction Time, MESH: Brain Injuries, MESH: Prefrontal Cortex, Neurology (clinical), [ SDV.BA.MVSA ] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Cognition Disorders, Neuroscience, MESH: Female, 030217 neurology & neurosurgery
Abstract: International audience; OBJECTIVE: To assess the cerebral correlates of the dysexecutive syndrome after diffuse severe traumatic brain injury (TBI). METHODS: Ten patients with sub-acute/chronic severe TBI without detectable focal cortical contusion and 11 matched healthy subjects were included in a parametric fMRI study using a planning task, the Tower of London. RESULTS: Brain activation in the left Dorsolateral Pre-frontal Cortex (DLPFC) and the Anterior Cingulate Cortex (ACC) was closely related to performance. Patients with TBI who performed the task efficiently showed, like healthy controls who obtained a similar pattern of performance, a large activation in the left DLPFC and a small activation in the ACC. In contrast, poor performance was associated with a reduced activation in these both regions. CONCLUSION: Problem-solving deficits after severe diffuse TBI could be related to an impaired activation of the DLPFC and of the ACC.
Published: 2006

24. Swallowing, arterial pulsation, and breathing induce motion artifacts in carotid artery MRI

Author: Loic Boussel, Philippe Douek, Jean Michel Serfaty, Gwenael Herigault, Michel Nonent, Alejandro de la Vega, Laboratoire Creatis, Compte Général, Hôpital Cardiovasculaire Louis Pradel ( HCLP ), Hospices Civils de Lyon ( HCL ), Philips Medical Systems ( PMS ), Philips Medical Systems, Département de radiologie [Brest] ( DR - Brest ), Centre Hospitalier Régional Universitaire de Brest ( CHRU Brest ), Groupe d'Etude de la Thrombose de Bretagne Occidentale ( GETBO ), Université de Brest ( UBO ), Département de Radiologie, Université Xavier Bichat, Hôpital Cardiovasculaire Louis Pradel (HCLP), Hospices Civils de Lyon (HCL), Philips Medical Systems (PMS), Département de radiologie [Brest] (DR - Brest), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Groupe d'Etude de la Thrombose de Bretagne Occidentale (GETBO), Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO), Centre de Recherche et d'Application en Traitement de l'Image et du Signal (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Carotid Artery Diseases, Male, MESH : Motion, MESH : Atherosclerosis, MESH : Deglutition, Pulsatile flow, MESH : Aged, MESH : Carotid Arteries, [INFO.INFO-IM] Computer Science [cs]/Medical Imaging, Magnetic resonance angiography, MESH : Artifacts, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, MESH: Atherosclerosis, 0302 clinical medicine, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, Image Processing, Computer-Assisted, MESH : Female, MESH: Aged, MESH: Middle Aged, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, medicine.diagnostic_test, Respiration, Isocenter, Middle Aged, MESH : Adult, Magnetic Resonance Imaging, MESH: Image Processing, Computer-Assisted, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Amplitude, Carotid Arteries, MESH: Artifacts, [INFO.INFO-TI] Computer Science [cs]/Image Processing [eess.IV], Pulsatile Flow, [INFO.INFO-TI]Computer Science [cs]/Image Processing [eess.IV], MESH: Pulsatile Flow, Cardiology, Breathing, Female, [INFO.INFO-MO] Computer Science [cs]/Modeling and Simulation, Artifacts, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, MESH : Image Processing, Computer-Assisted, Adult, medicine.medical_specialty, [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing, MESH: Motion, MESH : Male, Diastole, Lumen (anatomy), MESH : Pulsatile Flow, 03 medical and health sciences, Motion, Swallowing, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Internal medicine, MESH : Magnetic Resonance Imaging, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, medicine, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Humans, Radiology, Nuclear Medicine and imaging, MESH : Middle Aged, MESH : Carotid Artery Diseases, Aged, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, MESH: Respiration, MESH: Carotid Arteries, MESH: Humans, business.industry, MESH: Carotid Artery Diseases, MESH : Humans, MESH: Adult, Atherosclerosis, [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation, MESH: Male, Deglutition, MESH : Respiration, MESH: Deglutition, business, MESH: Female, 030217 neurology & neurosurgery, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Purpose To identify and quantify the potential sources of motion in carotid artery imaging. Materials and Methods Two healthy volunteers and 12 patients (20–75 years old) with atherosclerotic disease were scanned on a Philips Intera 1.5T system. A single-shot balanced-fast field echo (bFFE) sequence was used to acquire real-time axial views of the carotid artery wall (three images per second). A three-step acquisition protocol was performed to analyze the three types of motion (arterial pulsation, breathing, and swallowing) separately. The isocenter carotid artery motion amplitude in either the x or y direction was measured. Radial variation in the carotid lumen between the systolic and diastolic phases was analyzed. Motion frequency was reported for each patient. Results Significant motion related to arterial pulsation (amplitude = 0.27–0.93 mm, mean = 0.6, SD = 0.19), breathing (amplitude = 0.5–3.6 mm, mean = 1.56, SD = 0.99)), and swallowing (amplitude = 1.4–9.2 mm, mean = 4.7, SD = 2.4) were visualized. Conclusion Pulsation, breathing, and swallowing are sources of significant motion in the carotid artery wall. Such motion should be considered in the future to improve carotid artery image quality. J. Magn. Reson. Imaging 2006. © 2006 Wiley-Liss, Inc.
Published: 2006

25. A non-rigid registration approach for quantifying myocardial contraction in tagged MRI using generalized information measures

Author: Francoise Preteux, Nicolas Rougon, Philippe Grenier, Caroline Petitjean, Philippe Cluzel, Département Advanced Research And Techniques For Multidimensional Imaging Systems ( ARTEMIS ), Institut Mines-Télécom [Paris]-Télécom SudParis ( TSP ), Mathématiques Appliquées à Paris 5 ( MAP5 - UMR 8145 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National des Sciences Mathématiques et de leurs Interactions-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes ( LITIS ), Université Le Havre Normandie ( ULH ), Normandie Université ( NU ) -Normandie Université ( NU ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Institut national des sciences appliquées Rouen Normandie ( INSA Rouen Normandie ), Normandie Université ( NU ), École des Mines de Paris, Service de radiologie, Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Pitié-Salpêtrière [APHP], Service de Radiologie Polyvalente Diagnostique et Interventionnelle, Département Advanced Research And Techniques For Multidimensional Imaging Systems (ARTEMIS), Institut Mines-Télécom [Paris] (IMT)-Télécom SudParis (TSP), CHU Pitié-Salpêtrière [AP-HP], and Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)
Subjects: Male, MESH: Myocardial Contraction, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Myocardial Ischemia, MESH : Myocardial Ischemia, [ SPI.SIGNAL ] Engineering Sciences [physics]/Signal and Image processing, 030218 nuclear medicine & medical imaging, Mr tagging, 0302 clinical medicine, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, Image Processing, Computer-Assisted, Computer vision, MESH : Female, MR tagging, MESH : Algorithms, MESH : Myocardial Contraction, [ SDV.IB.IMA ] Life Sciences [q-bio]/Bioengineering/Imaging, Mathematics, Radiological and Ultrasound Technology, MESH: Magnetic Resonance Imaging, Cine, MESH : Adult, MESH : Magnetic Resonance Imaging, Cine, MESH: Image Processing, Computer-Assisted, Computer Graphics and Computer-Aided Design, MESH: Myocardial Ischemia, Female, Computer Vision and Pattern Recognition, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, Algorithms, MESH : Image Processing, Computer-Assisted, Adult, [ INFO.INFO-TS ] Computer Science [cs]/Signal and Image Processing, Computation, MESH : Male, Magnetic Resonance Imaging, Cine, MESH: Algorithms, Health Informatics, Non-rigid registration, 03 medical and health sciences, Motion estimation, Medical imaging, Humans, Radiology, Nuclear Medicine and imaging, MESH: Humans, business.industry, MESH : Humans, Cardiac motion analysis, Generalized information measures, MESH: Adult, Myocardial function, Myocardial Contraction, MESH: Male, Artificial intelligence, business, MESH: Female, 030217 neurology & neurosurgery
Abstract: International audience; We address the problem of quantitatively assessing myocardial function from tagged MRI sequences. We develop a two-step method comprising (i) a motion estimation step using a novel variational non-rigid registration technique based on generalized information measures, and (ii) a measurement step, yielding local and segmental deformation parameters over the whole myocardium. Experiments on healthy and pathological data demonstrate that this method delivers, within a reasonable computation time and in a fully unsupervised way, reliable measurements for normal subjects and quantitative pathology-specific information. Beyond cardiac MRI, this work redefines the foundations of variational non-rigid registration for information-theoretic similarity criteria with potential interest in multimodal medical imaging.
Published: 2005

26. 7-Ketocholesterol favors lipid accumulation and colocalizes with Nile Red positive cytoplasmic structures formed during 7-ketocholesterol-induced apoptosis: analysis by flow cytometry, FRET biphoton spectral imaging microscopy, and subcellular fractionation

Author: Ginette Bessède, Gérard Lizard, Edmond Kahn, Anne Vejux, Jean-Marc Riedinger, Andrew Todd-Pokropek, Anne Athias, Franck Ménétrier, Frédérique Frouin, Eric Ogier-Denis, Jean-François Stoltz, Dominique Dumas, Laboratoire de Biochimie Moléculaire et Cellulaire ( LBMC ), Université de Bourgogne ( UB ), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Laboratoire d'Imagerie Fonctionnelle ( LIF ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire d'Energétique et de Mécanique Théorique Appliquée ( LEMTA ), Université de Lorraine ( UL ) -Centre National de la Recherche Scientifique ( CNRS ), Plate-forme Imagerie et Biophysique Cellulaire et Tissulaire ( PTIBC-IBISA Nancy ), Université Henri Poincaré - Nancy 1 ( UHP ), Plateforme Lipidomique [Dijon] ( LAP ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ) -Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ) -IFR100 - Structure fédérative de recherche Santé-STIC-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Laboratoire de Biologie Médicale [Centre Georges-François leclerc], Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc ( CRLCC - CGFL ), Faculté de Médecine Xavier Bichat, Laboratoire de Biochimie Moléculaire et Cellulaire (LBMC), Université de Bourgogne (UB), Lipides - Nutrition - Cancer (U866) (LNC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire d'Imagerie Fonctionnelle (LIF), Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Énergies et Mécanique Théorique et Appliquée (LEMTA ), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Plate-forme Imagerie et Biophysique Cellulaire et Tissulaire (PTIBC-IBISA Nancy), Université Henri Poincaré - Nancy 1 (UHP), Plateforme Lipidomique [Dijon] (LAP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-IFR100 - Structure fédérative de recherche Santé-STIC-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER, Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), and Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-IFR100 - Structure fédérative de recherche Santé-STIC-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)
Subjects: MESH: Flow Cytometry, Apoptosis, 7. Clean energy, law.invention, MESH : Phosphatidylcholines, 0302 clinical medicine, Fluorescence Resonance Energy Transfer, MESH : Fluorescent Dyes, Enzyme Inhibitors, MESH: Lipid Metabolism, 0303 health sciences, MESH : Cell Nucleus, Flow Cytometry, MESH: Image Processing, Computer-Assisted, Cholesterol, MESH : Propidium, 030220 oncology & carcinogenesis, MESH : U937 Cells, MESH: Microscopy, Electron, Transmission, MESH: Cell Fractionation, MESH : Image Processing, Computer-Assisted, MESH: Propidium, Propidium, MESH: Cell Nucleus, MESH : Sphingomyelins, MESH: Mitochondria, Cell Fractionation, Filipin, Permeability, Pathology and Forensic Medicine, 03 medical and health sciences, Microscopy, Electron, Transmission, Oxazines, MESH: Membrane Potentials, Humans, Propidium iodide, MESH: Humans, MESH : Oxazines, MESH : Humans, Nile red, MESH: Phosphatidylcholines, Lipid Metabolism, Förster resonance energy transfer, chemistry, MESH: Subcellular Fractions, Cytoplasmic Structures, Benzimidazoles, MESH : Apoptosis, MESH: Fluorescence Resonance Energy Transfer, MESH: Sphingomyelins, Membrane Potentials, chemistry.chemical_compound, MESH: Cholesterol, [ SDV.BBM.BC ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], law, MESH : Lipid Metabolism, Image Processing, Computer-Assisted, Fluorescence microscope, MESH : Membrane Potentials, Ketocholesterols, MESH : Cholesterol, MESH: Ketocholesterols, U937 Cells, MESH: Fluorescent Dyes, Mitochondria, Sphingomyelins, MESH: Intracellular Membranes, MESH : Microscopy, Electron, Transmission, Biochemistry, MESH : Benzimidazoles, MESH: Enzyme Inhibitors, MESH: Permeability, MESH : Cell Fractionation, Phosphatidylcholines, lipids (amino acids, peptides, and proteins), MESH : Mitochondria, Cell fractionation, Subcellular Fractions, Histology, MESH : Flow Cytometry, MESH: U937 Cells, MESH : Subcellular Fractions, Confocal microscopy, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Fluorescent Dyes, 030304 developmental biology, MESH : Ketocholesterols, Cell Nucleus, MESH : Enzyme Inhibitors, MESH: Apoptosis, Lipid metabolism, Intracellular Membranes, Cell Biology, MESH : Cytoplasmic Structures, MESH: Cytoplasmic Structures, MESH : Intracellular Membranes, MESH : Permeability, MESH : Fluorescence Resonance Energy Transfer, MESH: Benzimidazoles, MESH: Oxazines
Abstract: Background Oxidized low-density lipoproteins play key roles in atherosclerosis. Their toxicity is at least in part due to 7-ketocholesterol (7KC), which is a potent inducer of apoptosis. In this study on human promonocytic U937 cells, we determined the effects and the interactions of 7KC with cellular lipids during 7KC-induced apoptosis. Methods Morphologic and functional changes were investigated by microscopic and flow cytometric methods after staining with propidium iodide, 3,3′-dihexyloxacarbocyanine iodide, and Hoechst 33342. Cellular lipid content was identified by using filipin to quantify free cholesterol and Nile Red (NR), which emit a yellow or orange-red fluorescence in the presence of neutral and polar lipids, respectively. After staining with NR, interactions of 7KC with cellular lipids were identified by fluorescence resonance energy transfer biphoton spectral imaging confocal microscopy and by subcellular fractionation, gas chromatography, and mass spectrometry. Results During 7KC-induced apoptosis the fluorescence from filipin and the ratio of measured (orange-red vs. yellow) fluorescence of NR were enhanced. Spectral analysis of images obtained in biphoton mode and resulting factor images demonstrated the occurrence of fluorescence resonance energy transfer between 7KC and NR and the subsequent colocalization of 7KC and NR. These data were in agreement with biochemical characterization and demonstrated that 7KC and neutral and polar lipids accumulate in NR-stained cytoplasmic structures. Conclusions During 7KC-induced apoptosis, 7KC modifies the cellular content of neutral and polar lipids, favors free cholesterol accumulation, and colocalizes with neutral and polar lipids that are inside NR-stained cytoplasmic structures. © 2005 Wiley-Liss, Inc.
Published: 2005

27. FRET multiphoton spectral imaging microscopy of 7-ketocholesterol and Nile Red in U937 monocytic cells loaded with 7-ketocholesterol

Author: Kahn, E., Vejux, A., Dumas, D., Montange, T., Frédérique Frouin, Robert, V., Riedinger, J. M., Stoltz, J. F., Gambert, P., Todd-Pokropek, A., Lizard, G., Laboratoire d'Imagerie Fonctionnelle ( LIF ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire de Biochimie Moléculaire et Cellulaire ( LBMC ), Université de Bourgogne ( UB ), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Laboratoire d'Energétique et de Mécanique Théorique Appliquée ( LEMTA ), Université de Lorraine ( UL ) -Centre National de la Recherche Scientifique ( CNRS ), Plate-forme Imagerie et Biophysique Cellulaire et Tissulaire ( PTIBC-IBISA Nancy ), Université Henri Poincaré - Nancy 1 ( UHP ), Analysis, perception and recognition of speech ( PAROLE ), INRIA Lorraine, Institut National de Recherche en Informatique et en Automatique ( Inria ) -Institut National de Recherche en Informatique et en Automatique ( Inria ) -Laboratoire Lorrain de Recherche en Informatique et ses Applications ( LORIA ), Institut National de Recherche en Informatique et en Automatique ( Inria ) -Université Henri Poincaré - Nancy 1 ( UHP ) -Université Nancy 2-Institut National Polytechnique de Lorraine ( INPL ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Henri Poincaré - Nancy 1 ( UHP ) -Université Nancy 2-Institut National Polytechnique de Lorraine ( INPL ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Biologie Médicale [Centre Georges-François leclerc], Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc ( CRLCC - CGFL ), Plateforme Lipidomique [Dijon] ( LAP ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ) -Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ) -IFR100 - Structure fédérative de recherche Santé-STIC-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Laboratoire d'Imagerie Fonctionnelle (LIF), Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biochimie Moléculaire et Cellulaire (LBMC), Université de Bourgogne (UB), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Laboratoire Énergies et Mécanique Théorique et Appliquée (LEMTA ), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Plate-forme Imagerie et Biophysique Cellulaire et Tissulaire (PTIBC-IBISA Nancy), Université Henri Poincaré - Nancy 1 (UHP), Analysis, perception and recognition of speech (PAROLE), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire Lorrain de Recherche en Informatique et ses Applications (LORIA), Institut National de Recherche en Informatique et en Automatique (Inria)-Université Henri Poincaré - Nancy 1 (UHP)-Université Nancy 2-Institut National Polytechnique de Lorraine (INPL)-Centre National de la Recherche Scientifique (CNRS)-Université Henri Poincaré - Nancy 1 (UHP)-Université Nancy 2-Institut National Polytechnique de Lorraine (INPL)-Centre National de la Recherche Scientifique (CNRS), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER, Plateforme Lipidomique [Dijon] (LAP), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-IFR100 - Structure fédérative de recherche Santé-STIC-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Dumas, Dominique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique de Lorraine (INPL)-Université Nancy 2-Université Henri Poincaré - Nancy 1 (UHP)-Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique de Lorraine (INPL)-Université Nancy 2-Université Henri Poincaré - Nancy 1 (UHP), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-IFR100 - Structure fédérative de recherche Santé-STIC-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)
Subjects: MESH: Cell Death, MESH: Fluorescence Resonance Energy Transfer, MESH: Mitochondria, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, MESH : Flow Cytometry, MESH: Flow Cytometry, MESH: U937 Cells, MESH: Monocytes, Monocytes, Membrane Potentials, MESH : Staining and Labeling, MESH : Microscopy, Fluorescence, Multiphoton, Oxazines, Fluorescence Resonance Energy Transfer, Image Processing, Computer-Assisted, Humans, MESH: Membrane Potentials, MESH: Microscopy, Confocal, MESH : Membrane Potentials, MESH : Fluorescent Dyes, MESH : Microscopy, Confocal, Ketocholesterols, [ SDV.IB.IMA ] Life Sciences [q-bio]/Bioengineering/Imaging, Fluorescent Dyes, MESH : Ketocholesterols, Microscopy, Confocal, MESH: Humans, MESH : Oxazines, Cell Death, Staining and Labeling, MESH : Humans, MESH: Ketocholesterols, U937 Cells, Flow Cytometry, MESH: Fluorescent Dyes, MESH: Image Processing, Computer-Assisted, Mitochondria, MESH: Staining and Labeling, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, Microscopy, Fluorescence, Multiphoton, MESH : Monocytes, MESH : Fluorescence Resonance Energy Transfer, MESH : Cell Death, MESH : U937 Cells, MESH: Microscopy, Fluorescence, Multiphoton, MESH : Mitochondria, MESH: Oxazines, MESH : Image Processing, Computer-Assisted
Abstract: To show the effect of 7-ketocholesterol (7KC) on cellular lipid content by means of flow cytometry and the interaction of 7KC with Nile Red (NR) via ultraviolet fluorescence resonance energy transfer (FRET) excitation of NR on U937 monocytic cells by means of 2-photon excitation confocal laser scanning microscopy (CLSM).Untreated and 7KC-treated U937 cells were stained with NR and analyzed by flow cytometry and CLSM. 3D sequences of images were obtained by spectral analysis in a 2-photon excitation CLSM and analyzed by the factor analysis of medical image sequences (FAMIS) algorithm, which provides factor curves and images. Factor images are the result of the FAMIS image processing method, which handles emission spectra. In FRET analysis, preparations are screened at selected UV wavelengths to avoid emission of NR in the absence of 7KC.During 7KC-induced cell death,flow cytometry and CLSM revealed a modification of the cellular lipid content. Factor images show FRET occurrence and subsequent colocalization of 7KC and NR.This investigation established the utility of 2-photon excitation CLSM to assess colocalization of 7KC with NR by FRET and to identify and distinguish polar and neutral lipids stained by NR that accumulate from the effect of 7KC.
Published: 2004

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27 results on '"MESH : Image Processing, Computer-Assisted"'

1. Genetic and Environmental Influences on the Visual Word Form and Fusiform Face Areas

2. Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells

3. Autobiographical memory in semantic dementia: new insights from two patients using fMRI

4. FISH-quant: automatic counting of transcripts in 3D FISH images

5. MRI, FDG-TEP and Florbetapir-TEP in AD

6. An adapted optical flow algorithm for robust quantification of cardiac wall motion from standard cine-MR examinations

7. Subjective cognition and amyloid deposition imaging: a Pittsburgh Compound B positron emission tomography study in normal elderly individuals

8. Sleep contributes to the strengthening of some memories over others, depending on hippocampal activity at learning. : Sleep, the hippocampus and memory consolidation

9. Sleep contributes to the strengthening of some memories over others, depending on hippocampal activity at learning

10. Mental time travel into the past and the future in healthy aged adults: an fMRI study. : Mental time travel into the past and the future

11. Mental time travel into the past and the future in healthy aged adults: an fMRI study. : Mental time travel into the past and the future

12. Validating an interlingual metanorm for emotional analysis of texts

13. Spatiotemporal motion estimation for respiratory-correlated imaging of the lungs

14. Decrease of prefrontal metabolism after subthalamic stimulation in obsessive-compulsive disorder: a positron emission tomography study

15. In situ protein expression in tumour spheres: development of an immunostaining protocol for confocal microscopy

16. Vestibular asymmetry as the cause of idiopathic scoliosis: a possible answer from Xenopus

17. Quantification of liver fat content: comparison of triple-echo chemical shift gradient-echo imaging and in vivo proton MR spectroscopy

18. [Radiochemotherapy for oesophageal cancer: a locoregional failure history]

19. Listeria monocytogenes EGD-e biofilms: no mushrooms but a network of knitted chains

20. Uncinate fasciculus fiber tracking in mesial temporal lobe epilepsy. Initial findings

21. An 11-year-old boy with drug-resistant schizophrenia treated with temporo-parietal rTMS

22. Measuring trunk orientation with a CMOS camera: feasibility and accuracy

23. fMRI study of problem-solving after severe traumatic brain injury

24. Swallowing, arterial pulsation, and breathing induce motion artifacts in carotid artery MRI

25. A non-rigid registration approach for quantifying myocardial contraction in tagged MRI using generalized information measures

26. 7-Ketocholesterol favors lipid accumulation and colocalizes with Nile Red positive cytoplasmic structures formed during 7-ketocholesterol-induced apoptosis: analysis by flow cytometry, FRET biphoton spectral imaging microscopy, and subcellular fractionation

27. FRET multiphoton spectral imaging microscopy of 7-ketocholesterol and Nile Red in U937 monocytic cells loaded with 7-ketocholesterol

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27 results on '"MESH : Image Processing, Computer-Assisted"'

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