1. Trm112p Is a 15-kDa Zinc Finger Protein Essential for the Activity of Two tRNA and One Protein Methyltransferases in Yeast
- Author
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Léon Dirick, Bruno Lapeyre, Glenn R. Björk, Marie-Hélène Mazauric, Suresh K. Purushothaman, Centre de recherches de biochimie macromoléculaire ( CRBM ), Université Montpellier 1 ( UM1 ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -IFR122-Centre National de la Recherche Scientifique ( CNRS ), Institut de Génétique Moléculaire de Montpellier ( IGMM ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Institut de Génétique Moléculaire de Montpellier (IGMM), and Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
- Subjects
Proteomics ,MESH : DNA ,MESH: tRNA Methyltransferases ,MESH : Saccharomyces cerevisiae ,MESH : Models, Genetic ,Biochemistry ,MESH: Recombinant Proteins ,MESH : Proteomics ,MESH: Saccharomyces cerevisiae Proteins ,MESH : Anticodon ,RNA, Transfer ,MESH : Catalysis ,MESH: Models, Genetic ,Zinc finger ,tRNA Methyltransferases ,MESH : Cell Nucleus ,0303 health sciences ,MESH : Chromatin ,MESH: Proteomics ,030302 biochemistry & molecular biology ,MESH: DNA ,Zinc Fingers ,MESH : Protein Binding ,Translation (biology) ,MESH: Saccharomyces cerevisiae ,MESH : Mitosis ,Chromatin ,Recombinant Proteins ,MESH : tRNA Methyltransferases ,Transfer RNA ,T arm ,MESH : Mutation ,Protein Binding ,MESH: Cell Nucleus ,TRNA modification ,Saccharomyces cerevisiae Proteins ,MESH: Mutation ,MESH : Recombinant Proteins ,Saccharomyces cerevisiae ,Mitosis ,MESH : RNA, Transfer ,Biology ,Catalysis ,Chromatin remodeling ,MESH: Chromatin ,MESH : Saccharomyces cerevisiae Proteins ,03 medical and health sciences ,Anticodon ,MESH: Anticodon ,MESH: Zinc Fingers ,MESH: Protein Binding ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,MESH : Zinc Fingers ,[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Molecular Biology ,030304 developmental biology ,Cell Nucleus ,Models, Genetic ,TRNA Methyltransferase ,DNA ,Cell Biology ,MESH: Mitosis ,MESH: Catalysis ,MESH: RNA, Transfer ,biology.organism_classification ,Mutation ,RNA - Abstract
International audience; The degenerate base at position 34 of the tRNA anticodon is the target of numerous modification enzymes. In Saccharomyces cerevisiae, five tRNAs exhibit a complex modification of uridine 34 (mcm(5)U(34) and mcm(5)s(2)U(34)), the formation of which requires at least 25 different proteins. The addition of the last methyl group is catalyzed by the methyltransferase Trm9p. Trm9p interacts with Trm112p, a 15-kDa protein with a zinc finger domain. Trm112p is essential for the activity of Trm11p, another tRNA methyltransferase, and for Mtq2p, an enzyme that methylates the translation termination factor eRF1/Sup45. Here, we report that Trm112p is required in vivo for the formation of mcm(5)U(34) and mcm(5)s(2)U(34). When produced in Escherichia coli, Trm112p forms a complex with Trm9p, which renders the latter soluble. This recombinant complex catalyzes the formation of mcm(5)U(34) on tRNA in vitro but not mcm(5)s(2)U(34). An mtq2-0 trm9-0 strain exhibits a synthetic growth defect, thus revealing the existence of an unexpected link between tRNA anticodon modification and termination of translation. Trm112p is associated with other partners involved in ribosome biogenesis and chromatin remodeling, suggesting that it has additional roles in the cell.
- Published
- 2010