1. Rnd family genes are differentially regulated by 3,4-methylenedioxymethamphetamine and cocaine acute treatment in mice brain
- Author
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Alexandre David, Cynthia Marie-Claire, Cindie Courtin, Florence Noble, Corinne Canestrelli, Julie Salzmann, Neuropsychopharmacologie des addictions. Vulnérabilité et variabilité expérimentale et clinique (NAVVEC - UM 81 (UMR 8206/ U705)), Université Paris Diderot - Paris 7 (UPD7)-Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Neuropsychopharmacologie des addictions. Vulnérabilité et variabilité expérimentale et clinique ( NAVVEC (UM 81) ), Université Paris Diderot - Paris 7 ( UPD7 ) -Institut des sciences du Médicament -Toxicologie - Chimie - Environnement ( IFR71 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL ( ENSCP ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL ( ENSCP ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Université Paris Diderot - Paris 7 (UPD7), and Marie-Claire, Cynthia
- Subjects
Male ,rho GTP-Binding Proteins ,MESH : RNA, Messenger ,MESH : Cocaine ,Time Factors ,MESH: Dopamine Uptake Inhibitors ,MESH : Brain Chemistry ,Hippocampus ,Striatum ,Pharmacology ,Mice ,0302 clinical medicine ,Cocaine ,Dopamine Uptake Inhibitors ,MESH: Up-Regulation ,MESH: Animals ,MESH: Brain Chemistry ,Enzyme Inhibitors ,Extracellular Signal-Regulated MAP Kinases ,Prefrontal cortex ,MESH : Up-Regulation ,MESH: Extracellular Signal-Regulated MAP Kinases ,MESH: Adrenergic Uptake Inhibitors ,MESH : Adrenergic Uptake Inhibitors ,0303 health sciences ,MESH : Gene Expression Regulation ,Adrenergic Uptake Inhibitors ,General Neuroscience ,Brain ,MDMA ,MESH : Extracellular Signal-Regulated MAP Kinases ,MESH: Cocaine-Related Disorders ,MESH: Gene Expression Regulation ,Up-Regulation ,3. Good health ,medicine.anatomical_structure ,[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,MESH: Enzyme Inhibitors ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,medicine.symptom ,MESH : Time Factors ,medicine.drug ,MESH : Male ,N-Methyl-3,4-methylenedioxyamphetamine ,Central nervous system ,Biology ,MESH : Cocaine-Related Disorders ,MESH: Brain ,Cocaine-Related Disorders ,03 medical and health sciences ,MESH: Cocaine ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,MESH : Mice ,MESH : rho GTP-Binding Proteins ,medicine ,Animals ,RNA, Messenger ,MESH : N-Methyl-3,4-methylenedioxyamphetamine ,MESH: Mice ,Molecular Biology ,MESH: RNA, Messenger ,030304 developmental biology ,Brain Chemistry ,MESH : Enzyme Inhibitors ,Rnd3 ,MESH: Time Factors ,MESH: rho GTP-Binding Proteins ,Actin cytoskeleton ,MESH: Male ,MESH : Brain ,Gene Expression Regulation ,Mechanism of action ,MESH: N-Methyl-3,4-methylenedioxyamphetamine ,MESH : Animals ,Neurology (clinical) ,MESH : Dopamine Uptake Inhibitors ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
International audience; Drugs of abuse induce alterations in cytoskeletal and cytoskeleton associated genes in several brain areas. We have previously shown that acute MDMA regulates the mRNA level of Rnd3, a Rho GTPase involved in actin cytoskeleton regulation, in mice striatum. In this study we investigated the effects of single administration of cocaine, another psychostimulant with a slightly different mechanism of action, on the mRNA levels of the three members of the Rnd genes family (Rnd1, Rnd2 and Rnd3). Mice were treated with either MDMA (9 mg/kg) or cocaine (20 mg/jg) and brain samples (i.e. hippocampus, striatum and prefrontal cortex) were processed for quantitative real-time PCR assay 1, 2, 4 and 6 h after the injections. The expression level of Rnd2 was differentially affected depending on the drug, brain area and time point after injection. Interestingly the two drugs up-regulate Rnd3 gene expression in the three structures tested with some differences in the timing. The effects of MDMA on Rnd3 appear earlier in the hippocampus as compared to cocaine, while it is the opposite in the prefrontal cortex. However, in the dorsal striatum, the two drugs induce an early and significant up-regulation of Rnd3 expression that is longer-lasting in the case of MDMA. In the case of cocaine contrarily to what was observed with MDMA, this modulation could not be blocked with the ERK activation inhibitor SL327 suggesting that the two drugs lead to the same effect on Rnd3 by two distinct pathways.
- Published
- 2007
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