1. Evaluation of the pro-angiogenic effect of nanoscale extracellular vesicles derived from human umbilical cord mesenchymal stem cells
- Author
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Gabriel Dostert, Sébastien Hupont, Patrick Menu, Véronique Decot, Emilie Velot, Huguette Louis, Anne-Sophie Willemin, Pierre Gillet, Valérie Jouan-Hureaux, Vanessa Moby, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Centre de Recherche en Automatique de Nancy (CRAN), Bioingénierie Moléculaire, Cellulaire et Thérapeutique (BMCT), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Défaillance Cardiovasculaire Aiguë et Chronique (DCAC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Faculté de Pharmacie [Nancy], and Université de Lorraine (UL)
- Subjects
0301 basic medicine ,Angiogenesis ,Cell Culture Techniques ,Regenerative medicine ,Umbilical cord ,Umbilical vein ,law.invention ,Umbilical Cord ,angiogenesis ,0302 clinical medicine ,law ,MESH: Angiogenesis Inducing Agents ,MESH: Human Umbilical Vein Endothelial Cells ,Cells, Cultured ,HUVECs ,medicine.diagnostic_test ,Chemistry ,General Medicine ,Cord lining ,3. Good health ,Cell biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,cardiovascular system ,MESH: Neovascularization, Physiologic ,MESH: Cells, Cultured ,Biomedical Engineering ,Neovascularization, Physiologic ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Flow cytometry ,Biomaterials ,03 medical and health sciences ,Extracellular Vesicles ,Confocal microscopy ,Nanoscale extracellular vesicles ,medicine ,Human Umbilical Vein Endothelial Cells ,Humans ,MESH: Particle Size ,Particle Size ,MESH: Extracellular Vesicles ,MESH: Cell Culture Techniques ,MESH: Humans ,MESH: Umbilical Cord ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,human umbilical cord MSCs ,030104 developmental biology ,MESH: Mesenchymal Stromal Cells ,Angiogenesis Inducing Agents - Abstract
Selected paper from the 6th China–France International Symposium “Stem Cells and Regenerative Medicine” and the first meeting of the France–China International CNRS Network (GDRI) CeSMeR, Nancy, 10–13 July 2016; International audience; Mesenchymal stem cells (MSCs) are a common tool in regenerative medicine. The nanoscale extracellular vesicles (nEVs) secreted by these cells were recently brought up to light thanks to their therapeutic potential. In this study, we assessed the in vitro behaviour of human umbilical vein endothelial cells (HUVECs) exposed to nEVs derived from human umbilical cord mesenchymal stem cells (hUC-MSCs). Nanoscale extracellular vesicles were isolated and characterized by NanoSight® and flow cytometry. HUVECs were stimulated with various concentrations of nEVs. To assess nEV interactions with HUVECs, confocal microscopy and angiogenesis assay were performed. The use of nEVs derived from hUC-MSCs was able to produce positive outcomes on HUVECs by acting on their angiogenic potential.
- Published
- 2017