1. Ultra-structural cell distribution of the melanoma marker iodobenzamide: improved potentiality of SIMS imaging in life sciences
- Author
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Jean-Luc Guerquin-Kern, Alain Croisy, Janine Papon, Jean-Claude Madelmont, Francois Hillion, Pierre Labarre, Maylin, Françoise, Biophysique moléculaire, Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), CAMECA France, Etude Métabolique des Molécules Marquées, Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Santé et de la Recherche Médicale (INSERM), and The NanoSims 50 was purchased with financial support from INSERM, the Research division of the Curie Institute, the 'Ile de France' district, the EDF radioprotection agency and ARC (contract n° 7401).
- Subjects
Pathology ,Microprobe ,Lung Neoplasms ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Melanoma, Experimental ,Spectrometry, Mass, Secondary Ion ,Lateral resolution ,MESH: Research Support, Non-U.S. Gov't ,030218 nuclear medicine & medical imaging ,chemistry.chemical_compound ,Mice ,MESH: Benzamides ,MESH: Cell Compartmentation ,0302 clinical medicine ,MESH: Melanins ,MESH: Animals ,0303 health sciences ,MESH: Melanosomes ,Melanosomes ,Radiological and Ultrasound Technology ,Melanoma ,MESH: Spectrometry, Mass, Secondary Ion ,General Medicine ,Secondary ion mass spectrometry ,MESH: Melanoma, Experimental ,lcsh:R855-855.5 ,Benzamides ,medicine.medical_specialty ,lcsh:Medical technology ,Materials science ,Biomedical Engineering ,Nanotechnology ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Models, Biological ,Biomaterials ,03 medical and health sciences ,Iodobenzamide ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,MESH: Mice, Inbred C57BL ,medicine ,Animals ,Radiology, Nuclear Medicine and imaging ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology ,MESH: Mice ,030304 developmental biology ,Melanins ,Research ,MESH: Models, Biological ,medicine.disease ,Cell Compartmentation ,[SDV.BIO] Life Sciences [q-bio]/Biotechnology ,MESH: Lung Neoplasms ,Mice, Inbred C57BL ,chemistry ,SIMS Microscopy ,Neoplasm Transplantation ,MESH: Neoplasm Transplantation - Abstract
Background Analytical imaging by secondary ion mass spectrometry (SIMS) provides images representative of the distribution of a specific ion within a sample surface. For the last fifteen years, concerted collaborative research to design a new ion microprobe with high technical standards in both mass and lateral resolution as well as in sensitivity has led to the CAMECA NanoSims 50, recently introduced onto the market. This instrument has decisive capabilities, which allow biological applications of SIMS microscopy at a level previously inaccessible. Its potential is illustrated here by the demonstration of the specific affinity of a melanoma marker for melanin. This finding is of great importance for the diagnosis and/or treatment of malignant melanoma, a tumour whose worldwide incidence is continuously growing. Methods The characteristics of the instrument are briefly described and an example of application is given. This example deals with the intracellular localization of an iodo-benzamide used as a diagnostic tool for the scintigraphic detection of melanic cells (e.g. metastasis of malignant melanoma). B16 melanoma cells were injected intravenously to C57BL6/J1/co mice. Multiple B16 melanoma colonies developed in the lungs of treated animals within three weeks. Iodobenzamide was injected intravenously in tumour bearing mice six hours before sacrifice. Small pieces of lung were prepared for SIMS analysis. Results Mouse lung B16 melanoma colonies were observed with high lateral resolution. Cyanide ions gave "histological" images of the cell, representative of the distribution of C and N containing molecules (e.g. proteins, nucleic acids, melanin, etc.) while phosphorus ions are mainly produced by nucleic acids. Iodine was detected only in melanosomes, confirming the specific affinity of the drug for melanin. No drug was found in normal lung tissue. Conclusion This study demonstrates the potential of SIMS microscopy, which allows the study of ultra structural distribution of a drug within a cell. On the basis of our observations, drug internalization via membrane sigma receptors can be excluded.
- Published
- 2004