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3 results on '"MESH: Listeriosis/microbiology"'

1. Listeria monocytogenes-associated respiratory infections: a study of 38 consecutive cases

Author

M. Morgand, Mylène M. Maury, Caroline Charlier, Alexandre Leclercq, Pierre Thouvenot, Guillaume Vales, Hélène Bracq-Dieye, Marc Lecuit, Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Biologie des Infections - Biology of Infection, Université Paris Descartes - Paris 5 (UPD5), Funding was received from the Institut Pasteur, Santé Publique, France., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), and DIAKITE, andrée

Subjects
Lung Diseases, Male, 0301 basic medicine, MESH: Respiratory Tract Infections/epidemiology, MESH: Listeria monocytogenes/isolation & purification, MESH: Empyema, Pleural/microbiology, Pleural effusion, MESH: Empyema, Pleural/etiology, medicine.medical_treatment, [SDV]Life Sciences [q-bio], MESH: Empyema, Pleural/drug therapy, MESH: Listeriosis/epidemiology, MESH: Listeriosis/drug therapy, MESH: Aged, 80 and over, listeriosis, pleural effusion, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Sepsis/etiology, MESH: Anti-Bacterial Agents/therapeutic use, Respiratory Tract Infections, MESH: Respiratory Tract Infections/etiology, Aged, 80 and over, MESH: Aged, Pleuropneumonia, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, MESH: Middle Aged, Respiratory infection, Immunosuppression, General Medicine, Middle Aged, MESH: Pneumonia, Bacterial/drug therapy, MESH: Pneumonia, Bacterial/etiology, MESH: Sepsis/epidemiology, Anti-Bacterial Agents, 3. Good health, [SDV] Life Sciences [q-bio], Infectious Diseases, medicine.anatomical_structure, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Lung Diseases/microbiology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Pneumonia, Bacterial/microbiology, Female, MESH: Empyema, Pleural/epidemiology, medicine.drug, Adult, Microbiology (medical), MESH: Pleuropneumonia/microbiology, MESH: Pneumonia, Bacterial/epidemiology, MESH: Lung Diseases/epidemiology, medicine.medical_specialty, 030106 microbiology, MESH: Pleuropneumonia/drug therapy, 03 medical and health sciences, MESH: Listeriosis/complications, Sepsis, Internal medicine, Pneumonia, Bacterial, medicine, Humans, pneumonia, MESH: Sepsis/drug therapy, MESH: Lung Diseases/drug therapy, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Respiratory Tract Infections/drug therapy, Empyema, Pleural, Aged, Retrospective Studies, MESH: Pleuropneumonia/etiology, MESH: Sepsis/microbiology, Lung, MESH: Humans, business.industry, MESH: Respiratory Tract Infections/microbiology, MESH: Adult, MESH: Retrospective Studies, Amoxicillin, medicine.disease, Listeria monocytogenes, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Empyema, MESH: Male, Pneumonia, MESH: Lung Diseases/etiology, Concomitant, MESH: Listeriosis/microbiology, MESH: Listeria monocytogenes/drug effects, MESH: Pleuropneumonia/epidemiology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, business, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

Listeria monocytogenes (Lm) is a foodborne human pathogen responsible for severe infections, including septicaemia, neurolisteriosis, and maternal-foetal and focal infections. Little is known about Lm-associated respiratory tract or lung infections.We conducted a retrospective study of culture-proven cases of Lm pleural infections and pneumonia reported to the French National Reference Centre for Listeria from January 1993 to August 2016.Thirty-eight consecutive patients with pleural infection (n = 32), pneumonia (n = 5), or both (n = 1) were studied; 71% of these were men. Median age was 72 (range 29-90). Two patients presented with concomitant neurolisteriosis. All patients but one reported at least one immunosuppressive condition (97%), with a median number of 2 (range 0-5), including 29% (8/28) with current exposure to immunosuppressive therapy and 50% (17/34) with ongoing neoplasia; 75% (21/28) reported previous pleural or pulmonary disease. Antibiotic therapy mostly consisted in amoxicillin (72%) associated with aminoglycoside in 32%. Chest-tube drainage was performed in 7/19 patients with empyema (37%); 25% of the patients (7/30) required intensive care management. In-hospital mortality reached 35% and occurred after a median time interval of 4 days (range 1-33 days). Three patients had recurrence of empyema (time interval of 1 week to 4 months after treatment completion). Altogether, only 13/31 patients (42%) diagnosed with Lm respiratory infection experienced an uneventful outcome at 2-year follow-up.Lm is a rare but severe cause of pneumonia and pleural infection in older immunocompromised patients, requiring prompt diagnosis and adequate management and follow-up.

Published
2018
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2. Comparison of widely used Listeria monocytogenes strains EGD, 10403S, and EGD-e highlights genomic variations underlying differences in pathogenicity

Author

Bécavin, Christophe, Bouchier, Christiane, Lechat, Pierre, Archambaud, Cristel, Creno, Sophie, Gouin, Edith, Wu, Zongfu, Kühbacher, Andreas, Brisse, Sylvain, Pucciarelli, M. Graciela, García-del Portillo, Francisco, Hain, Torsten, Portnoy, Daniel A., Chakraborty, Trinad, Lecuit, Marc, Pizarro-Cerdá, Javier, Moszer, Ivan, Bierne, Hélène, Cossart, Pascale, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Génomique (Plate-Forme) - Genomics Platform, Institut Pasteur [Paris], Bioanalyse génomique (Plate-Forme), Génomique évolutive des Microbes / Microbial Evolutionary Genomics, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centro de Biología Molecular Severo Ochoa [Madrid] (CBMSO), Universidad Autonoma de Madrid (UAM)-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Centro Nacional de Biotecnología (CSIC), Institut für Medizinische Mikrobiologie - Institute of Medical Microbiology, Justus-Liebig-Universität Gießen (JLU), Department of Molecular and Cell Biology and School of Public Health, Biologie des Infections - Biology of Infection, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Université Paris Descartes - Paris 5 (UPD5), Centre d'infectiologie Necker-Pasteur [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut Pasteur [Paris], ANR-2010-PATH ERA-NET, This work received financial support from the European Research Council(advanced grant 233348), the French Agence Nationale de la Recherche(grants BACNET 10-BINF-02-01, IBEID ANR-10-LABX-62-01, andERA-NET ANR-2010-PATH), the Institut Pasteur, the Institut Nationalde la Santé et de la Recherche Médicale, and the Institut National de laRecherche Agronomique, ANR-10-BINF-0002,BACNET,Exploring composition and dynamics of bacterial regulatory networks(2010), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 233348,MODELIST, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Universidad Autónoma de Madrid (UAM)-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Centro Nacional de Biotecnología [Madrid] (CNB-CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Justus-Liebig-Universität Gießen = Justus Liebig University (JLU), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Casadevall, Arturo, vicente, marie-therese, Exploring composition and dynamics of bacterial regulatory networks - - BACNET2010 - ANR-10-BINF-0002 - BINF - VALID, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, ERC Advanced grant - MODELIST - 233348 - INCOMING, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Génomique (Plate-Forme), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Centro de Biología Molecular Severo Ochoa (CBMSO), Universidad Autonoma de Madrid (UAM)-Consejo Superior de Investigaciones Científicas [Spain] (CSIC), Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), ANR: 10-BINF-02-01,BACNET,ANR, ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), and ANR: ERA-NET,ANR-2010-PATH

Subjects
MESH: Liver/microbiology, MESH: Sequence Analysis, DNA, MESH: Spleen/microbiology, internalin, model, genes, in-vivo, t-cells, e-cadherin, surface-proteins, intestinal barrier, controls expression, virulence regulator prfa, MESH: Virulence, MESH: Genome, Bacterial, Mice, 2.2 Factors relating to the physical environment, 2.1 Biological and endogenous factors, Listeriosis, MESH: Animals, MESH: Genetic Variation, Aetiology, Inbred BALB C, MESH: Listeriosis/pathology, Genome, Virulence, Bacterial, Foodborne Illness, Blood, Infectious Diseases, Liver, MESH: Listeria monocytogenes/genetics, MESH: Blood/microbiology, Infection, Sequence Analysis, Molecular Sequence Data, MESH: Mice, Inbred BALB C, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Microbiology, Vaccine Related, MESH: DNA, Bacterial/chemistry, Biodefense, Genetics, Animals, Humans, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, MESH: Mice, MESH: Molecular Sequence Data, MESH: Humans, Animal, Prevention, Human Genome, MESH: Listeria monocytogenes/pathogenicity, Genetic Variation, DNA, Listeria monocytogenes, MESH: DNA, Bacterial/genetics, Emerging Infectious Diseases, Disease Models, MESH: Listeriosis/microbiology, MESH: Disease Models, Animal, Digestive Diseases, Spleen
Abstract

International audience; For nearly 3 decades, listeriologists and immunologists have used mainly three strains of the same serovar (1/2a) to analyze the virulence of the bacterial pathogen Listeria monocytogenes. The genomes of two of these strains, EGD-e and 10403S, were released in 2001 and 2008, respectively. Here we report the genome sequence of the third reference strain, EGD, and extensive genomic and phenotypic comparisons of the three strains. Strikingly, EGD-e is genetically highly distinct from EGD (29,016 single nucleotide polymorphisms [SNPs]) and 10403S (30,296 SNPs), and is more related to serovar 1/2c than 1/2a strains. We also found that while EGD and 10403S strains are genetically very close (317 SNPs), EGD has a point mutation in the transcriptional regulator PrfA (PrfA*), leading to constitutive expression of several major virulence genes. We generated an EGD-e PrfA* mutant and showed that EGD behaves like this strain in vitro, with slower growth in broth and higher invasiveness in human cells than those of EGD-e and 10403S. In contrast, bacterial counts in blood, liver, and spleen during infection in mice revealed that EGD and 10403S are less virulent than EGD-e, which is itself less virulent than EGD-e PrfA*. Thus, constitutive expression of PrfA-regulated virulence genes does not appear to provide a significant advantage to the EGD strain during infection in vivo, highlighting the fact that in vitro invasion assays are not sufficient for evaluating the pathogenic potential of L. monocytogenes strains. Together, our results pave the way for deciphering unexplained differences or discrepancies in experiments using different L. monocytogenes strains. IMPORTANCE Over the past 3 decades, Listeria has become a model organism for host-pathogen interactions, leading to critical discoveries in a broad range of fields, including bacterial gene regulation, cell biology, and bacterial pathophysiology. Scientists studying Listeria use primarily three pathogenic strains: EGD, EGD-e, and 10403S. Despite many studies on EGD, it is the only one of the three strains whose genome has not been sequenced. Here we report the sequence of its genome and a series of important genomic and phenotypic differences between the three strains, in particular, a critical mutation in EGD's PrfA, the main regulator of Listeria virulence. Our results show that the three strains display differences which may play an important role in the virulence differences observed between the strains. Our findings will be of critical relevance to listeriologists and immunologists who have used or may use Listeria as a tool to study the pathophysiology of listeriosis and immune responses.

Published
2014
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3. Complete genome sequence of the animal pathogen Listeria ivanovii, which provides insights into host specificities and evolution of the genus Listeria

Author

Carmen Buchrieser, Torsten Hain, Uwe Kärst, Werner Goebel, Christophe Rusniok, José A. Vázquez-Boland, Robert Lampidis, Juergen Kreft, Mariela Scortti, Pascale Cossart, P. Garrido, Trinad Chakraborty, Philippe Glaser, Biologie des bactéries intracellulaires, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Evolution et Génomique Bactériennes, Grupo de Patogénesis Molecular Bacteriana [Madrid], Facultad de Veterinaria, Universidad Complutense, Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM)-Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Institut für Medizinische Mikrobiologie - Institute of Medical Microbiology, Justus-Liebig-Universität Gießen = Justus Liebig University (JLU), Microbial Pathogenesis Unit., University of Edinburgh, Biocenter University of Würzburg = Biozentrum der Universität Würzburg, Julius-Maximilians-Universität Würzburg (JMU), Helmholtz-Zentrum für Infektionsforschung GmbH (HZI), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Ludwig-Maximilians-Universität München (LMU), This work was supported by the Institut Pasteur, the Centre National de Recherche Scientifique (CNRS), the German Federal Ministry for Education and Research (BMBF) through the Competence Network PathoGenoMik (031U213B), the European Union (FP6), and the Spanish Ministry for Science and Innovation (GEN2006-27774). Work in the laboratory of J.A.V.-B. is supported by The Wellcome Trust., Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Justus-Liebig-Universität Gießen (JLU), Julius-Maximilians-Universität Würzburg [Wurtzbourg, Allemagne] (JMU), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Ludwig-Maximilians University [Munich] (LMU), HOFFMANN, Isabelle, Universidad Complutense de Madrid [Madrid] (UCM)-Universidad Complutense de Madrid [Madrid] (UCM), Biocenter University of Würzburg, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA)

Subjects
Serotype, OUTBREAK, genome sequence, Genome browser, MESH: Base Sequence, Genome, MESH: Listeria/isolation & purification, MESH: Host Specificity, Listeriosis, MESH: Animals, Pathogen, 0303 health sciences, biology, GASTROENTERITIS, Strain (biology), listeria ivanovii, Ruminants, Genome Announcements, animal pathogen, MESH: Listeria/genetics, Listeria ivanovii, genus listeria, Listeria, Molecular Sequence Data, MONOCYTOGENES, MESH: Listeria/classification, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Microbiology, complex mixtures, Host Specificity, MESH: Listeriosis/veterinary, Evolution, Molecular, 03 medical and health sciences, parasitic diseases, MESH: Evolution, Molecular, Animals, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Molecular Biology, 030304 developmental biology, Whole genome sequencing, MESH: Molecular Sequence Data, Base Sequence, MESH: Listeria/physiology, 030306 microbiology, microbiology, MESH: Genome, Bacterial, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Ruminants/microbiology, MESH: Listeriosis/microbiology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Genome, Bacterial
Abstract

International audience; We report the complete and annotated genome sequence of the animal pathogen Listeria ivanovii subsp. ivanovii strain PAM 55 (serotype 5), isolated in 1997 in Spain from an outbreak of abortion in sheep. The sequence and its analysis are available at an interactive genome browser at the Institut Pasteur (http://genolist.pasteur.fr/LivaList/).

Published
2011
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