Descriptor: "MESH: Listeriosis" - Searchworks@Jio Institute Digital Library Search Results

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52 results on '"MESH: Listeriosis"'

1. Listeria monocytogenes: Investigation of Fitness in Soil Does Not Support the Relevance of Ecotypes

Author: Yann Sévellec, Eliette Ascencio, Pierre-Emmanuel Douarre, Benjamin Félix, Laurent Gal, Dominique Garmyn, Laurent Guillier, Pascal Piveteau, Sophie Roussel, Laboratoire de sécurité des aliments de Maisons-Alfort (LSAl), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Agroécologie [Dijon], Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Direction de l'Evaluation des Risques (DER), Optimisation des procédés en Agriculture, Agroalimentaire et Environnement (UR OPAALE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and European Project: 773830, H2020-SFS-2017-1 ,One Health EJP(2018)
Subjects: Microbiology (medical), clonal complex, ecotype, strain origin, [SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study, MESH: Listeria monocytogenes, Listeria monocytogenes, genome-wide-association study, survival, Microbiology, fitness, soil, MESH: Soil, MESH: Listeriosis, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Foodborne Diseases
Abstract: Listeria monocytogenes (Lm) is a ubiquitous bacterium that causes the serious foodborne illness listeriosis. Although soil is a primary reservoir and a central habitat for Lm, little information is available on the genetic features underlying the fitness of Lm strains in this complex habitat. The aim of this study was to identify (i) correlations between the strains fitness in soil, their origin and their phylogenetic position (ii) identify genetic markers allowing Lm to survive in the soil. To this end, we assembled a balanced panel of 216 Lm strains isolated from three major ecological compartments (outdoor environment, animal hosts, and food) and from 33 clonal complexes occurring worldwide. The ability of the 216 strains to survive in soil was tested phenotypically. Hierarchical clustering identified three phenotypic groups according to the survival rate (SR): phenotype 1 “poor survivors” (SR < 2%), phenotype 2 “moderate survivors” (2% < SR < 5%) and phenotype 3 “good survivors” (SR > 5%). Survival in soil depended neither on strains’ origin nor on their phylogenetic position. Genome-wide-association studies demonstrated that a greater number of genes specifically associated with a good survival in soil was found in lineage II strains (57 genes) than in lineage I strains (28 genes). Soil fitness was mainly associated with variations in genes (i) coding membrane proteins, transcription regulators, and stress resistance genes in both lineages (ii) coding proteins related to motility and (iii) of the category “phage-related genes.” The cumulative effect of these small genomic variations resulted in significant increase of soil fitness.
Published: 2022
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2. A European-wide dataset to uncover adaptive traits of Listeria monocytogenes to diverse ecological niches

Author: Korkealä, Hannu J., Karpíšková, Renáta, Gareis, Manfred, Ruppitsch, W., Hurtado, Ana, Lindström, Miia K., Guillier, Laurent, Cammà, Cesare, Hébraud, Michel, Blanchard, Yannick, Pomilio, Francesco, Gelbíčová, Tereza, Roussel, Sophie, Piveteau, Pascal, Bridier, Arnaud, Pietzka, Ariane Tatjana, Soumet, Christophe, Félix, Benjamin, Torresi, Marina, Radomski, Nicolas, Seyfarth, Anne Mette, Skjerdal, Taran, Wullings, Bart A., Di Pasquale, Adriano, Papić, Bojan, Douarre, Pierre Emmanuel, Felten, Arnaud, Mallet, Ludovic V., Palma, Federica, Szymczak, Barbara, Kramarenko, Toomas, Sévellec, Yann, Šteingolde, Žanete, Leroux, Aurélie, Castro, Hanna, Bulawová, Hana, Oswaldi, Verena, Cabanová, Lenka, Ascensio, Eliette, Canelhas, Monica Ricão, Leblanc, Jean Charles, Marti, Elisabet, Food Hygiene and Environmental Health, Departments of Faculty of Veterinary Medicine, University of Helsinki, Helsinki One Health (HOH), Laboratoire de sécurité des aliments de Maisons-Alfort (LSAl), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Laboratoire de Fougères - ANSES, Optimisation des procédés en Agriculture, Agroalimentaire et Environnement (UR OPAALE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Microbiologie Environnement Digestif Santé (MEDIS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Veterinary Research Institute [Brno] (VRI), Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise Guiseppe Caporale (IZSAM), Partenaires INRAE, Norwegian Veterinary Institute [Oslo], Austrian Agency for Health and Food Safety (AGES), Swedish Food Agency, University of Ljubljana, Neiker-Instituto Vasco de Investigación y Desarrollo Agrario (SPAIN), Instituto Vasco de Investigación y Desarrollo Agrario [Derio] (NEIKER), Basque Research and Technology Alliance (BRTA), Wageningen Food Safety Research, Wageningen (WFSR), State Veterinary Institute (SVU), Department of Food Hygiene and Environmental Health [Helsinki], Faculty of Veterinary Medicine [Helsinki], Helsingin yliopisto = Helsingfors universitet = University of Helsinki-Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Institute of Food Safety, Animal Health and Environment (BIOR), Veterinary and Food Laboratory, 51006 Tartu, Estonia, Department of Molecular Biology and Epizootiology, Veterinary and Food Institute in Dolny Kubin, State Veterinary and Food Institute, NRL for Honeybee Health, 026 01 Dolny Kubin, Slovakia, West Pomeranian University of Technology Szczecin, Ludwig Maximilian University [Munich] (LMU), Freie Universität Berlin, Agroscope, National Food Institute [Lyngby] (Forside), and European Project: 773830, H2020-SFS-2017-1 ,One Health EJP(2018)
Subjects: ecological niches, 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche, Moleculaire Biologie & AMR, MESH: Listeria monocytogenes, 413 Veterinary science, Molecular Biology & AMR, génétique, Foodborne Diseases, Bacterial genetics, dataset, Listeriosis, bacteria, bactérie, transmission, Computer Science Applications, CONTAMINATION, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, épidémiologie, epidemiology, Statistics, Probability and Uncertainty, niches écologiques, Information Systems, Statistics and Probability, food-borne pathogen, Library and Information Sciences, écosystème, Education, Micro 3: Knowledge and Expertise, SDG 3 - Good Health and Well-being, Team Bacteriology, données de la recherche, Life Science, Animals, Team Bacteriologie, human, microbiologie, SDG 2 - Zero Hunger, sécurité des aliments, MESH: Foodborne Diseases, Ecosystem, Team Bacteriologie, Moleculaire Biologie & AMR, [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], Team Bacteriology, Molecular Biology & AMR, STRAINS, microbiology, fungi, Bacteriology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Listeria monocytogenes, SOIL, MESH: Listeriosis, PORK, genetic, humain, Micro 3: Kennis en Expertise, pathogène alimentaire, CLONES
Abstract: Listeria monocytogenes (Lm) is a ubiquitous bacterium that causes listeriosis, a serious foodborne illness. In the nature-to-human transmission route, Lm can prosper in various ecological niches. Soil and decaying organic matter are its primary reservoirs. Certain clonal complexes (CCs) are over-represented in food production and represent a challenge to food safety. To gain new understanding of Lm adaptation mechanisms in food, the genetic background of strains found in animals and environment should be investigated in comparison to that of food strains. Twenty-one partners, including food, environment, veterinary and public health laboratories, constructed a dataset of 1484 genomes originating from Lm strains collected in 19 European countries. This dataset encompasses a large number of CCs occurring worldwide, covers many diverse habitats and is balanced between ecological compartments and geographic regions. The dataset presented here will contribute to improve our understanding of Lm ecology and should aid in the surveillance of Lm. This dataset provides a basis for the discovery of the genetic traits underlying Lm adaptation to different ecological niches.
Published: 2022
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3. Listeria spp. Isolated from Tonsils of Wild Deer and Boars: Genomic Characterization

Author: Carla Palacios-Gorba, Juan J. Quereda, Marc Lecuit, Jesús Gomis, María L. Mocé, Ángel Gómez-Martín, Alexandre Leclercq, E. Jiménez-Trigos, Alexandra Moura, Universidad Cardenal Herrera-CEU (CEU-UCH), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), This work was supported by Generalitat Valenciana (project reference GV/2018/A/183) and by the Spanish Ministry of Science and Innovation (project reference PID2019-110764RA-I00) (J.J.Q.), Institut Pasteur, Inserm, and Santé Publique France. J. J. Quereda is supported by a 'Ramón y Cajal' contract of the Spanish Ministry of Science, Innovation and Universities (RYC-2018-024985-I). C. Palacios-Gorba is supported by a predoctoral contract from the Universidad Cardenal Herrera-CEU., We thank Hélène Bracq-Dieye, Nathalie Tessaud-Rita, Pierre Thouvenot, and Guillaume Vales (Institut Pasteur, Paris, France) for DNA extraction and the P2M platform (Institut Pasteur, Paris, France) for genome sequencing., DIAKITE, andrée, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)
Subjects: [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV]Life Sciences [q-bio], medicine.disease_cause, MESH: Genome, Bacterial, Applied Microbiology and Biotechnology, MESH: Listeria, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Animals, MESH: Phylogeny, Pathogen, 2. Zero hunger, 0303 health sciences, education.field_of_study, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Ecology, Public and Environmental Health Microbiology, [SDV.BA]Life Sciences [q-bio]/Animal biology, MESH: Feces, 3. Good health, wild animal, [SDV] Life Sciences [q-bio], MESH: Multilocus Sequence Typing, MESH: Palatine Tonsil, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Listeria ivanovii, MESH: Whole Genome Sequencing, Biotechnology, Population, MESH: Deer, Biology, Microbiology, 03 medical and health sciences, Listeria monocytogenes, medicine, education, Feces, 030304 developmental biology, 030306 microbiology, biology.organism_classification, Pathogenicity island, MESH: Sus scrofa, virulence, MESH: Listeriosis, tonsil, Listeria, Multilocus sequence typing, cgMLST, WGS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Food Science
Abstract: Listeria monocytogenes is a major human and animal foodborne pathogen. However, data from environmental reservoirs remain scarce. Here, we used whole-genome sequencing to characterize Listeria species isolates recovered over 1 year from wild animals in their natural habitats in Spain. Three different Listeria spp. (L. monocytogenes [n = 19], Listeria ivanovii subsp. londoniensis [n = 4], and Listeria innocua [n = 3]) were detected in 23 animal tonsils (9 deer, 14 wild boars) and 2 feeding troughs. No Listeria species was detected in feces. L. monocytogenes was detected in tonsils of 44.4% (8 out of 18) of deer and 40.7% (11 out of 27) of wild boars. L. monocytogenes isolates belonged to 3 different core genome multilocus sequence typing (cgMLST) types (CTs) of 3 distinct sublineages (SL1, SL387, and SL155) from lineages I and II. While cgMLST type L1-SL1-ST1-CT5279 (IVb; clonal complex 1 [CC1]) occurred only in one animal, types L1-SL387-ST388-CT5239 (IVb; CC388) and L2-SL155-ST155-CT1170 (IIa; CC155) were retrieved from multiple animals. In addition, L1-SL387-ST388-CT5239 (IVb; CC388) isolates were collected 1 year apart, revealing their long-term occurrence within the animal population and/or environmental reservoir. The presence of identical L. monocytogenes strains in deer and wild boars suggests contamination from a common food or environmental source, although interhost transmission cannot be excluded. Pathogenicity islands LIPI-1, LIPI-3, and LIPI-4 were present in 100%, 5%, and 79% of the L. monocytogenes isolates, respectively, and all L. monocytogenes lineage II isolates (n = 3) carried SSI-1 stress islands. This study highlights the need for monitoring L. monocytogenes environmental contamination and the importance of tonsils as a possible L. monocytogenes intrahost reservoir. IMPORTANCEListeria monocytogenes is a foodborne bacterial pathogen responsible for listeriosis. Whole-genome sequencing has been extensively used in public health and food industries to characterize circulating Listeria isolates, but genomic data on isolates occurring in natural environments and wild animals are still scarce. Here, we show that wild animals carry pathogenic Listeria and that the same genotypes can be found at different time points in different host species. This work highlights the need of Listeria species monitoring of environmental contamination and the importance of tonsils as a possible L. monocytogenes intrahost reservoir.
Published: 2021
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4. Emerging Evasion Mechanisms of Macrophage Defenses by Pathogenic Bacteria

Author: Clarisse Leseigneur, Pierre Lê-Bury, Javier Pizarro-Cerdá, Olivier Dussurget, Yersinia, Institut Pasteur [Paris] (IP), Université Paris Cité (UPCité), Centre National de Référence de la Peste et autres Yersinioses - National Reference Center Plague and Yersinioses (CNR), Centre collaborateur de l'OMS Yersinia - WHO Collaborating Center Yersinia (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Work on this topic was supported by Institut Pasteur, Université de Paris, Direction Générale de l'Armement, Agence Innovation Défense and Agence Nationale de la Recherche (ANR-17-CE18-0011-02). The Yersinia Research Unit is a member of the Laboratory of Excellence Integrative Biology of Emerging Infectious Diseases (ANR LBX-62-IBEID)., ANR-17-CE18-0011,NADKiller,NADK : une nouvelle cible bactérienne pour le développement d'antibiotiques(2017), and ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
Subjects: 0301 basic medicine, Microbiology (medical), Staphylococcus aureus, Mini Review, 030106 microbiology, Immunology, lcsh:QR1-502, Yersinia, MESH: Listeria monocytogenes, medicine.disease_cause, Microbiology, lcsh:Microbiology, 03 medical and health sciences, Cellular and Infection Microbiology, Immune system, listeriosis, Listeria monocytogenes, yersiniosis, MESH: Staphylococcus aureus, medicine, Humans, Macrophage, MESH: Immune Evasion, Immune Evasion, staphylococcal infection, MESH: Humans, biology, Macrophages, phagocyte, immune escape, MESH: Macrophages, Yersiniosis, Pathogenic bacteria, medicine.disease, biology.organism_classification, Evasion (ethics), [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, plague, virulence, 030104 developmental biology, Infectious Diseases, MESH: Listeriosis
Abstract: International audience; Macrophages participate to the first line of defense against infectious agents. Microbial pathogens evolved sophisticated mechanisms to escape macrophage killing. Here, we review recent discoveries and emerging concepts on bacterial molecular strategies to subvert macrophage immune responses. We focus on the expanding number of fascinating subversive tools developed by Listeria monocytogenes, Staphylococcus aureus, and pathogenic Yersinia spp., illustrating diversity and commonality in mechanisms used by microorganisms with different pathogenic lifestyles.
Published: 2020
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5. Pathogenic Biohacking: Induction, Modulation and Subversion of Host Transcriptional Responses by Listeria monocytogenes

Author: Matthew J. G. Eldridge, Melanie Hamon, Pascale Cossart, Chromatine et Infection - Chromatin and Infection, Institut Pasteur [Paris] (IP), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), M.J.G.E. is supported by a fellowship from the French Government’s Investissement d’Avenir program, the Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (ANR-10-LABX-62-IBEID). P.C. is funded by the Fondation le Roch les Mousquetaires and the Fondation Balzan. Work in the Chromatin and Infection Group is supported by the Pasteur Institute and the Agence National de la Recherche (ANR-EpiBActIn)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-17-CE12-0007,EpiBactIn,Modifications epigenomiques induites par l'interaction hote-bacteries(2017), Institut Pasteur [Paris], Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Martin, Marie, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, and Modifications epigenomiques induites par l'interaction hote-bacteries - - EpiBactIn2017 - ANR-17-CE12-0007 - AAPG2017 - VALID
Subjects: MESH: Signal Transduction, Listeria, Health, Toxicology and Mutagenesis, [SDV]Life Sciences [q-bio], MESH: Inflammation Mediators, Virulence, lcsh:Medicine, MESH: Virulence, Toxicology, medicine.disease_cause, MESH: Cellular Reprogramming, MESH: Listeria monocytogenes, virulence factor, Virulence factor, Microbiology, 03 medical and health sciences, Listeria monocytogenes, Gene expression, medicine, MESH: Animals, Epigenetics, MESH: Epigenesis, Genetic, Pathogen, Gene, transcription factor, 030304 developmental biology, MESH: Virulence Factors, 0303 health sciences, MESH: Humans, biology, epigenetics, 030306 microbiology, MESH: Transcription, Genetic, lcsh:R, MESH: Host-Pathogen Interactions, MESH: Transcription Factors, biology.organism_classification, [SDV] Life Sciences [q-bio], MESH: Listeriosis, gene expression
Abstract: International audience; During infection, the foodborne bacterial pathogen Listeria monocytogenes dynamically influences the gene expression profile of host cells. Infection-induced transcriptional changes are a typical feature of the host-response to bacteria and contribute to the activation of protective genes such as inflammatory cytokines. However, by using specialized virulence factors, bacterial pathogens can target signaling pathways, transcription factors, and epigenetic mechanisms to alter host gene expression, thereby reprogramming the response to infection. Therefore, the transcriptional profile that is established in the host is delicately balanced between antibacterial responses and pathogenesis, where any change in host gene expression might significantly influence the outcome of infection. In this review, we discuss the known transcriptional and epigenetic processes that are engaged during Listeria monocytogenes infection, the virulence factors that can remodel them, and the impact these processes have on the outcome of infection.
Published: 2020
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6. Listeria monocytogenes, a model in infection biology

Author: Marc Lecuit, Biologie des Infections - Biology of Infection, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Université de Paris (UP), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Agence Nationale de la RechercheH2020 European Research CouncilInsermInstitut Pasteur, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Université Paris Cité (UPCité), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)
Subjects: Listeria, [SDV]Life Sciences [q-bio], Immunology, Cellular microbiology, Context (language use), Biology, medicine.disease_cause, MESH: Listeria monocytogenes, Microbiology, Mice, 03 medical and health sciences, Infection biology, Listeria monocytogenes, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Sepsis, Virology, cell biology, medicine, Animals, Humans, Listeriosis, MESH: Animals, MESH: Food Microbiology, MESH: Mice, MESH: Sepsis, 030304 developmental biology, 0303 health sciences, MESH: Humans, 030306 microbiology, biology, animal model, pathogenesis, MESH: Host-Pathogen Interactions, Outbreak, Meningoencephalitis, medicine.disease, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, infection, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Listeriosis, Host-Pathogen Interactions, Food Microbiology, Identification (biology), [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Listeria monocytogenes causes listeriosis, a systemic infection which manifests as bacteremia, often complicated by meningoencephalitis in immunocompromised individuals and the elderly, and fetal-placental infection in pregnant women. It has emerged over the past decades as a major foodborne pathogen, responsible for numerous outbreaks in Western countries, and more recently in Africa. L. monocytogenes' pathogenic properties have been studied in detail, thanks to concomitant advances in biological sciences, in particular molecular biology, cell biology and immunology. L. monocytogenes has also been instrumental to basic advances in life sciences. L. monocytogenes therefore stands both a tool to understand biology and a model in infection biology. This review briefly summarises the clinical and some of the pathophysiological features of listeriosis. In the context of this special issue, it highlights some of the major discoveries made by Pascale Cossart in the fields of molecular and cellular microbiology since the mid-eighties regarding the identification and characterisation of multiple bacterial and host factors critical to L. monocytogenes pathogenicity. It also briefly summarises some of the key findings from our laboratory on this topic over the past years.
Published: 2020
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7. Atypical Hemolytic Listeria innocua Isolates Are Virulent, albeit Less than Listeria monocytogenes

Author: Alexandra Moura, Alexandre Leclercq, Morgane Lavina, Olivier Disson, Athmanya K. Eshwar, Marc Lecuit, Maria Fredriksson-Ahomaa, Pierre Thouvenot, Lei Huang, Roger Stephan, Centre National de Référence Listeria - Biologie des Infections (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), Biologie des Infections - Biology of Infection, Université Paris Diderot, Sorbonne Paris Cité, Paris, France, Université Paris Diderot - Paris 7 (UPD7), University of Helsinki, Institute for Food Safety and Hygiene, University of Zürich [Zürich] (UZH), Service des Maladies infectieuses et tropicales [CHU Necker], Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris], Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Institut des Maladies Génétiques Imagine [Paris], Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Universität Zürich [Zürich] = University of Zurich (UZH), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), University of Zurich, Freitag, Nancy E, and Lecuit, Marc
Subjects: inlA, L innocua, [SDV]Life Sciences [q-bio], 2405 Parasitology, LIPI, MESH: Virulence, medicine.disease_cause, MESH: Genome, Bacterial, MESH: Listeria monocytogenes, MESH: Bird Diseases, Feces, listeriosis, MESH: Listeria, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Ducks, MESH: Genomic Islands, MESH: Animals, Galliformes, MESH: Phylogeny, MESH: Bacterial Proteins, Phylogeny, 2. Zero hunger, 0303 health sciences, biology, Strain (chemistry), 2404 Microbiology, MESH: Feces, MESH: Galliformes, 3. Good health, Infectious Diseases, medicine.anatomical_structure, Ducks, MESH: Whole Genome Sequencing, Genomic Islands, Listeria, Immunology, Virulence, 610 Medicine & health, Spleen, Microbiology, 03 medical and health sciences, Listeria monocytogenes, Bacterial Proteins, medicine, Animals, Humans, Internalin, Serotyping, Gene, 10082 Institute of Food Safety and Hygiene, 030304 developmental biology, 2403 Immunology, MESH: Humans, Whole Genome Sequencing, 030306 microbiology, Bird Diseases, MESH: Serotyping, 2725 Infectious Diseases, biology.organism_classification, Pathogenicity island, Molecular Pathogenesis, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, virulence, L. innocua, MESH: Listeriosis, 570 Life sciences, Parasitology, Genome, Bacterial, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Listeria innocua is considered a nonpathogenic Listeria species. Natural atypical hemolytic L. innocua isolates have been reported but have not been characterized in detail. Here, we report the genomic and functional characterization of representative isolates from the two known natural hemolytic L. innocua clades. Whole-genome sequencing confirmed the presence of Listeria pathogenicity islands (LIPI) characteristic of Listeria monocytogenes species. Functional assays showed that LIPI-1 and inlA genes are transcribed, and the corresponding gene products are expressed and functional. Using in vitro and in vivo assays, we show that atypical hemolytic L. innocua is virulent, can actively cross the intestinal epithelium, and spreads systemi-cally to the liver and spleen, albeit to a lesser degree than the reference L. monocy-togenes EGDe strain. Although human exposure to hemolytic L. innocua is likely rare, these findings are important for food safety and public health. The presence of viru-lence traits in some L. innocua clades supports the existence of a common virulent ancestor of L. monocytogenes and L. innocua.
Published: 2019
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8. Virulence potential of Listeria monocytogenes strains recovered from pigs in Spain

Author: Jaime Gómez-Laguna, Javier Pizarro-Cerdá, Juan J. Quereda, Jazmín Meza-Torres, Fernando Cardoso-Toset, Universidad de Córdoba = University of Córdoba [Córdoba], Centro de investigación agroaliementaria - Food Research Center [Córdoba, Spain] (CICAP), Yersinia, Institut Pasteur [Paris] (IP), Centre National de Référence de la Peste et autres Yersinioses - National Reference Center Plague and Yersinioses (CNR), Universidad Cardenal Herrera-CEU (CEU-UCH), This study was supported by the Agency for Innovation and Development of Andalucía (IDEA, and project reference 351504/550859), by the University of Córdoba Research Program (Spain), by the Andalusian FEDER Operational Funding Program, by Generalitat Valenciana (project reference GV/2018/A/183) and by the Spanish Ministry of Science and Innovation (project reference PID2019- 110764RA- I00). JG- L is supported by a ’Ramón y Cajal’ contract of the Spanish Ministry of Economy and Competitiveness (RYC-2014-16735). JJQ is supported by a ’Ramón y Cajal’ contract of the Spanish Ministry of Science, Innovation and Universities (RYC-2018-024985- I). The funding bodies had no role in the design of the study, in the collection, analysis and interpretation of data, and in writing the manuscript.
Subjects: Serotype, replication, MESH: Swine Diseases, 040301 veterinary sciences, Virulence, MESH: Virulence, Biology, MESH: Listeria monocytogenes, medicine.disease_cause, virulence factor, Virulence factor, Microbiology, 0403 veterinary science, meat, Listeria monocytogenes, MESH: Spain, medicine, MESH: Animals, Internalin, MESH: Food Microbiology, MESH: Swine, Pathogen, MESH: Humans, General Veterinary, MESH: Pork Meat, 0402 animal and dairy science, Listeriolysin O, Outbreak, 04 agricultural and veterinary sciences, General Medicine, invasion, 040201 dairy & animal science, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, isolates, MESH: Listeriosis, tonsil
Abstract: International audience; Background: Listeria monocytogenes is a foodborne bacterial pathogen that causes listeriosis, an infectious disease of animals and humans, with pigs acting as asymptomatic reservoirs. In August 2019 a listeriosis outbreak associated with the consumption of pork meat caused 222 human cases in Spain. Determining the diversity as well as the virulence potential of strains from pig origin is important for public health concerns. Methods: The behavior of twenty-three L. monocytogenes strains recovered from pig tonsils, meat and skin was compared by studying: (1) Internalin A (InlA), Internalin B (InlB), Listeriolysin O (LLO), Actin assembly-inducing protein (ActA) and PrfA expression levels, and (2) invasion and intracellular growth in eukaryotic cells. Results: Marked differences were found in the expression of the selected virulence factors and the invasion and intracellular replication phenotypes of the L. monocytogenes strains. Strains obtained from meat samples and belonging to ST 1/2a did not have InlA anchored to the peptidoglycan. Some strains expressed higher levels of the studied virulence factors and invaded and replicated intracellularly more efficiently than an epidemic L. monocytogenes reference strain (F2365). Conclusion: This study demonstrates the presence of virulent L. monocytogenes strains in pigs, with valuable implications in veterinary medicine and food safety.
Published: 2020
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9. Cross-border outbreak of listeriosis caused by cold-smoked salmon, revealed by integrated surveillance and whole genome sequencing (WGS), Denmark and France, 2015 to 2017

Author: Sofie Gillesberg Lassen, Alexandre Leclercq, Alexandra Moura, Eva Møller Nielsen, Steen Ethelberg, Stine Thielke, Luise Müller, Marc Lecuit, Jette Kjeldgaard, T. Jensen, Mylène M. Maury, Mathieu Tourdjman, Marie-Pierre Donguy, Susanne Schjørring, Statens Serum Institut [Copenhagen], Danish Veterinary and Food Administration, Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de Référence Listeria - Biologie des Infections (CNRL), Technical University of Denmark [Lyngby] (DTU), Santé publique France, Direction Générale de l'Alimentation (DGAL), Ministère de l'agriculture, de l'agroalimentaire et de la forêt, Biologie des Infections - Biology of Infection, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Institut des Maladies Génétiques Imagine [Paris], Part of this work was supported by the European Union’s Horizon 2020 research and innovation program through COMPARE under grant agreement No. 643476., We thank the Danish Clinical Microbiological Laboratories for rapid referral of isolates and support of the surveillance system. In addition, we thank the physicians and other health personnel for supplying clinical information, Marie Walther, Charlotte Munkstrup, Louise La Barbera Kastberg, for their assistance in interviewing patients. Furthermore, we thank Christian Vråby Pedersen and Anette Holm (SSI), Anne Mette Seyfarth (DFVA) the French National reference center technicians, and the PIBnet platform (Institut Pasteur) for excellent technical expertise in performing whole-genome sequencing. We also thank the staff at the DFVA Laboratory in Ringsted, Denmark and the regional food inspection units for collecting the environment and product samples, European Project: 643476,H2020,H2020-PHC-2014-single-stage,COMPARE(2014), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Danmarks Tekniske Universitet = Technical University of Denmark (DTU), Santé publique France - French National Public Health Agency [Saint-Maurice, France], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)
Subjects: Male, 0301 basic medicine, food-borne infections, MESH: Salmon, MESH: Sequence Analysis, DNA, Epidemiology, Denmark, MESH: Emigration and Immigration, [SDV]Life Sciences [q-bio], MESH: Genome, Bacterial, MESH: Listeria monocytogenes, Genome, Disease Outbreaks, Foodborne Diseases, MESH: Aged, 80 and over, listeriosis, Salmon, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Animals, MESH: Disease Outbreaks, media_common, cold-smoked salmon, Aged, 80 and over, MESH: Aged, Molecular Epidemiology, whole genome sequencing, MESH: Middle Aged, Cross-border outbreak, Emigration and Immigration, Middle Aged, 3. Good health, Smoked salmon, Geography, MESH: Multilocus Sequence Typing, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, surveillance, Female, France, MESH: Whole Genome Sequencing, Rapid Communication, Adult, 030106 microbiology, Zoology, Disease cluster, 03 medical and health sciences, food, Virology, Animals, Humans, media_common.cataloged_instance, MESH: Molecular Epidemiology, European union, MESH: Foodborne Diseases, Aged, Whole genome sequencing, MESH: Denmark, MESH: Humans, Molecular epidemiology, Public Health, Environmental and Occupational Health, Outbreak, MESH: Adult, Sequence Analysis, DNA, Listeria monocytogenes, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, food.food, MESH: Male, MESH: France, Smoked fish, 030104 developmental biology, MESH: Listeriosis, MESH: Female, Genome, Bacterial, WGS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Multilocus Sequence Typing
Abstract: International audience; In August 2017, an outbreak of six listeriosis cases in Denmark was traced to cold-smoked salmon, using epidemiological investigations and whole-genome sequencing (WGS) analyses. Exchange of genome sequences allowed identification in France of a food isolate from a salmon-derived product and a human isolate from 2016 within the same cgMLST cluster as the Danish isolates (L2-SL8-ST8-CT771). The salmon product came from a third European Union country. WGS can rapidly link human cases and food isolates across Europe.
Published: 2017
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10. Listeria monocytogenes switches from dissemination to persistence by adopting a vacuolar lifestyle in epithelial cells

Author: Christine Péchoux, Gabriel Mitchell, Eliane Milohanic, Marie-Christine Prévost, Pascale Cossart, Hélène Bierne, Mounia Kortebi, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Lawrence Berkeley National Laboratory [Berkeley] (LBNL), Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Microscopie électronique (Plate-forme), Institut Pasteur [Paris], Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), The work of HB is supported by the French National Research Agency (ANR 11 BSV3 003 01, EPILIS), INRA (dept. MICA grant AO Blanc 2010), the French Ligue Nationale Contre le Cancer (comité régional d’Ile–de-France, LNCC 131/12) and the iXcore Foundation for Research. The work of MK is supported by a grant from the Lidex ALIAS of Université Paris- Saclay and by INRA (dept. MICA). GM is a postdoctoral scholar in the laboratory of Daniel A. Portnoy. Daniel A. Portnoy is supported by National Institutes of Health grants 1P01 AI063302 and 1R01 AI027655. GM is also supported by grants from the Fonds de Recherche du Québec - Santé (FRQS) and the Natural Sciences and Engineering Research Council of Canada (NSERC). PC acknowledges support from INFECT-ERA (PROANTILIS) and Labex IBEID., We are very grateful to Dan Portnoy for supporting this work and for helpful conversations. We thank L. Radoshevich for the critical reading of this manuscript, G. Lakisic for assistance, S. Aymerich for material support, E. Gouin for the generation of Listeria and ActA antibodies, T. Yoshimori for the gift of plasmid pEGFP-LC3, J. Swanson for the gift of pCBD-YFP, J.D. Sauer for the gift of mCherry-10403S, L. Travier, T. Couderc and M. Lecuit for the gift of EGDe-ΔactA+actA and ATG7 and BECN1 siRNAs, V. Libri for help in FACS procedures, and ImagGif and M. Metheule for help in TEM., ANR-11-BSV3-0003,EPILIS,Reprogrammation épigénétique par la bactérie pathogène Listeria monocytogenes(2011), ANR-13-IFEC-0004,PROANTILIS,Subversive pro- and anti-inflammation trade-offs promote infection by Listeria monocytogenes(2013), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 321529,EC:FP7:HEALTH,FP7-ERANET-2012-RTD,INFECT-ERA(2013), Institut Pasteur [Paris] (IP), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Kortebi, Mounia, Milohanic, Eliane, Bierne, Hélène, vicente, marie-therese, BLANC - Reprogrammation épigénétique par la bactérie pathogène Listeria monocytogenes - - EPILIS2011 - ANR-11-BSV3-0003 - BLANC - VALID, ERA-NET Infect-ERA - Subversive pro- and anti-inflammation trade-offs promote infection by Listeria monocytogenes - - PROANTILIS2013 - ANR-13-IFEC-0004 - IFEC - VALID, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Coordination of European funding for infectious diseases research - INFECT-ERA - - EC:FP7:HEALTH2013-01-01 - 2016-12-31 - 321529 - VALID, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), and ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010)
Subjects: 0301 basic medicine, Cytoplasm, Host cells, Vacuole, MESH: Heat-Shock Proteins, medicine.disease_cause, Pathology and Laboratory Medicine, MESH: Listeria monocytogenes, Hemolysin Proteins, Cytosol, Animal Cells, Medicine and Health Sciences, Listeriosis, Pathogen, lcsh:QH301-705.5, MESH: Bacterial Proteins, Heat-Shock Proteins, Staining, biology, Microbiology and Parasitology, Cell staining, Microbiologie et Parasitologie, Bacterial Pathogens, Liver, MESH: Hemolysin Proteins, Medical Microbiology, MESH: Epithelial Cells, MESH: Membrane Proteins, Pathogens, Cellular Structures and Organelles, Cellular Types, Anatomy, Research Article, lcsh:Immunologic diseases. Allergy, Listeria, Immunology, Motility, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Research and Analysis Methods, Microbiology, MESH: Vacuoles, Incubation period, Cell Line, 03 medical and health sciences, Listeria monocytogenes, Bacterial Proteins, Virology, Genetics, medicine, Humans, Molecular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Microbial Pathogens, Intracellular pathogens, MESH: Humans, Bacteria, Intracellular parasite, MESH: Cytoplasm, Organisms, Biology and Life Sciences, Membrane Proteins, Epithelial Cells, Cell Biology, biology.organism_classification, MESH: Cell Line, 030104 developmental biology, lcsh:Biology (General), Specimen Preparation and Treatment, MESH: Listeriosis, Vacuoles, Hepatocytes, Parasitology, lcsh:RC581-607, Viral Transmission and Infection
Abstract: Listeria monocytogenes causes listeriosis, a foodborne disease that poses serious risks to fetuses, newborns and immunocompromised adults. This intracellular bacterial pathogen proliferates in the host cytosol and exploits the host actin polymerization machinery to spread from cell-to-cell and disseminate in the host. Here, we report that during several days of infection in human hepatocytes or trophoblast cells, L. monocytogenes switches from this active motile lifestyle to a stage of persistence in vacuoles. Upon intercellular spread, bacteria gradually stopped producing the actin-nucleating protein ActA and became trapped in lysosome-like vacuoles termed Listeria-Containing Vacuoles (LisCVs). Subpopulations of bacteria resisted degradation in LisCVs and entered a slow/non-replicative state. During the subculture of host cells harboring LisCVs, bacteria showed a capacity to cycle between the vacuolar and the actin-based motility stages. When ActA was absent, such as in ΔactA mutants, vacuolar bacteria parasitized host cells in the so-called “viable but non-culturable” state (VBNC), preventing their detection by conventional colony counting methods. The exposure of infected cells to high doses of gentamicin did not trigger the formation of LisCVs, but selected for vacuolar and VBNC bacteria. Together, these results reveal the ability of L. monocytogenes to enter a persistent state in a subset of epithelial cells, which may favor the asymptomatic carriage of this pathogen, lengthen the incubation period of listeriosis, and promote bacterial survival during antibiotic therapy., Author summary L. monocytogenes is a model intracellular pathogen that replicates in the cytoplasm of mammalian cells and disseminate in the host using actin-based motility. Here, we reveal that L. monocytogenes changes its lifestyle and persists in lysosomal vacuoles during long-term infection of human hepatocytes and trophoblast cells. When the virulence factor ActA is not expressed, subpopulations of vacuolar bacteria enter a dormant viable but non-culturable (VBNC) state. This novel facet of the L. monocytogenes intracellular life could contribute to the asymptomatic carriage of this pathogen in epithelial tissues and render it tolerant to antibiotic therapy and undetectable by routine culture techniques.
Published: 2017
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11. Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis

Author: Lilliana Radoshevich, Pascale Cossart, Interactions Bactéries-Cellules (UIBC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), The authors gratefully acknowledge financial support from the European Research Area Network (ERA-NET) Infect-ERA BACVIRISG15 and PROANTILIS, the European Research Council (ERC) Advanced Grant BacCellEpi (670823), Agence Nationale de la Recherche (ANR) BACNET (10-BINF-02-01), ANR Investissement d'Avenir Programme (10-LABX-62-IBEID), Human Frontier Science Program (HFSP, RGP001/2013), the Balzan Foundation, the Pasteur-Weizmann Council and the Fondation le Roch les Mousquetaires. P.C. is a senior international research scholar of the Howard Hughes Medical Institute, USA., The authors thank D. Ribet, J.J. Quereda, S. Brisse and M. Lecuit for allowing us to adapt their figures. The authors thank H. Bierne, J. Pizarro-Cérda and O. Dussurget for helpful discussions., ANR-13-IFEC-0004,PROANTILIS,Subversive pro- and anti-inflammation trade-offs promote infection by Listeria monocytogenes(2013), ANR-10-BINF-02-01/10-BINF-0002,BACNET,BACNET(2010), ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), European Project: 670823,H2020,ERC-2014-ADG,BacCellEpi(2015), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), ANR-10-BINF-0002,BACNET,Exploring composition and dynamics of bacterial regulatory networks(2010), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: 0301 basic medicine, Bacterial immune evasion, 030106 microbiology, Physiology, Virulence, Bacterial adhesion, Disease, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, MESH: Virulence, medicine.disease_cause, MESH: Listeria monocytogenes, Microbiology, Bacterial evolution, 03 medical and health sciences, Antibiotic resistance, Listeria monocytogenes, Immunity, Bacterial genetics, medicine, Bacterial transcription, MESH: Animals, Cellular microbiology, Pathogen, MESH: Virulence Factors, MESH: Humans, General Immunology and Microbiology, Effector, Host (biology), MESH: Host-Pathogen Interactions, Antimicrobial responses, Bacterial pathogenesis, Bacterial host response, 3. Good health, Infectious Diseases, MESH: Listeriosis, Pathogens
Abstract: International audience; Listeria monocytogenes is a food-borne pathogen responsible for a disease called listeriosis, which is potentially lethal in immunocompromised individuals. This bacterium, first used as a model to study cell-mediated immunity, has emerged over the past 20 years as a paradigm in infection biology, cell biology and fundamental microbiology. In this Review, we highlight recent advances in the understanding of human listeriosis and L. monocytogenes biology. We describe unsuspected modes of hijacking host cell biology, ranging from changes in organelle morphology to direct effects on host transcription via a new class of bacterial effectors called nucleomodulins. We then discuss advances in understanding infection in vivo, including the discovery of tissue-specific virulence factors and the 'arms race' among bacteria competing for a niche in the microbiota. Finally, we describe the complexity of bacterial regulation and physiology, incorporating new insights into the mechanisms of action of a series of riboregulators that are critical for efficient metabolic regulation, antibiotic resistance and interspecies competition.
Published: 2017
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12. Spontaneous Loss of Virulence in Natural Populations of Listeria monocytogenes

Author: Mariela Scortti, Alexandra Moura, Lei Han, Guillaume Vales, Alexandre Leclercq, Olivier Disson, Marc Lecuit, Hélène Bracq-Dieye, Mylène M. Maury, Edith Gouin, José A. Vázquez-Boland, Viviane Chenal-Francisque, Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Edinburgh, Biologie des Infections - Biology of Infection, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Work in M.L.'s laboratory was supported by Institut Pasteur, Inserm, LabEx IBEID, the European Research Council, and Santé Publique France. Work in J.A.V.-B.'s laboratory was supported by the Wellcome Trust (program grant WT074020MA) and partially by core Roslin Institute funding from BBSRC (BB/J004227/1), We acknowledge Anne Morvan, Thomas Cantinelli, Nathalie Tessaud-Rita and Laetitia Bellon for their help in strain collection and isolation at the National Reference Centre for Listeria. We thank Pascale Cossart for providing antibodies against LLO and the Institut Pasteur P2M platform for genome sequencing., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), DIAKITE, andrée, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), Centre National de Référence Listeria - Biologie des Infections (CNRL), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Institut des Maladies Génétiques Imagine [Paris], and ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010)
Subjects: 0301 basic medicine, Erythrocytes, MESH: Selection, Genetic, Mutant, MESH: Heat-Shock Proteins, MESH: Virulence, medicine.disease_cause, MESH: Listeria monocytogenes, Severity of Illness Index, MESH: Recombinant Proteins, Hemolysin Proteins, Mice, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Listeriosis, MESH: Animals, Cloning, Molecular, MESH: Phylogeny, Pathogen, MESH: Bacterial Proteins, Heat-Shock Proteins, Phylogeny, Mice, Inbred BALB C, MESH: Gene Expression Regulation, Bacterial, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, MESH: Erythrocytes, Listeriolysin O, Biological Evolution, spontaneous mutations, MESH: Amino Acid Substitution, MESH: Hemolysis, Recombinant Proteins, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Hemolysin Proteins, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Peptide Termination Factors, Peptide Termination Factors, Transposable element, MESH: Mutation, Bacterial Toxins, 030106 microbiology, Immunology, MESH: Mice, Inbred BALB C, Virulence, Molecular Genomics, MESH: Biological Evolution, Biology, Microbiology, Frameshift mutation, 03 medical and health sciences, Bacterial Proteins, Listeria monocytogenes, MESH: Severity of Illness Index, medicine, genomics, Animals, Humans, MESH: Cloning, Molecular, Selection, Genetic, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Gene, MESH: Mice, MESH: Humans, Gene Expression Regulation, Bacterial, biochemical phenomena, metabolism, and nutrition, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, virulence, Amino Acid Substitution, MESH: Bacterial Toxins, MESH: Listeriosis, Mutation, Parasitology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, hemolysis, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: The pathogenesis of Listeria monocytogenes depends on the ability of this bacterium to escape from the phagosome of the host cells via the action of the pore-forming toxin listeriolysin O (LLO). Expression of the LLO-encoding gene ( hly ) requires the transcriptional activator PrfA, and both hly and prfA genes are essential for L. monocytogenes virulence. Here, we used the hemolytic activity of LLO as a phenotypic marker to screen for spontaneous virulence-attenuating mutations in L. monocytogenes . Sixty nonhemolytic isolates were identified among a collection of 57,820 confirmed L. monocytogenes strains isolated from a variety of sources (0.1%). In most cases (56/60; 93.3%), the nonhemolytic phenotype resulted from nonsense, missense, or frameshift mutations in prfA . Five strains carried hly mutations leading to a single amino acid substitution (G299V) or a premature stop codon causing strong virulence attenuation in mice. In one strain, both hly and gshF (encoding a glutathione synthase required for full PrfA activity) were missing due to genomic rearrangements likely caused by a transposable element. The PrfA/LLO loss-of-function (PrfA − /LLO − ) mutants belonged to phylogenetically diverse clades of L. monocytogenes , and most were identified among nonclinical strains (57/60). Consistent with the rare occurrence of loss-of-virulence mutations, we show that prfA and hly are under purifying selection. Although occurring at a low frequency, PrfA − /LLO − mutational events in L. monocytogenes lead to niche restriction and open an evolutionary path for obligate saprophytism in this facultative intracellular pathogen.
Published: 2017
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13. Alteration of host lysosomes by bacterial toxins

Author: Malet, Julien Karim, Cossart, Pascale, Ribet, David, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur, INSERM, INRA, National Research Agency, Grant/Award Number: (ANR, ERANET Infect‐ERA PROANTILIS ANR‐13‐IFEC‐0004‐02). French Government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases', Grant/Award Number: (ANR‐10‐LABX‐62‐IBEID). European Research Council (ERC), Grant/Award Number: (Advanced Grant #233348 MODELIST, H2020‐ERC‐2014‐ADG 670823‐BacCellEpi). Fondation Louis‐Jeantet, Fondation le Roch les Mousquetaires., We thank J. Gruenberg and R. Schekman for the helpful discussion, M. Hamon for the help with flow cytometry experiments, J. J. Quereda for the construction of the Listeria EGD Δhly strain and F. Stavru for the construction of the GFP-tagged Listeria EGD Δhly strain., ANR-13-IFEC-0004,PROANTILIS,Subversive pro- and anti-inflammation trade-offs promote infection by Listeria monocytogenes(2013), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 233348,EC:FP7:ERC,ERC-2008-AdG,MODELIST(2009), European Project: 670823,H2020,ERC-2014-ADG,BacCellEpi(2015), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), vicente, marie-therese, ERA-NET Infect-ERA - Subversive pro- and anti-inflammation trade-offs promote infection by Listeria monocytogenes - - PROANTILIS2013 - ANR-13-IFEC-0004 - IFEC - VALID, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Understanding the infection by the bacterium Listeria monocytogenes as a way to address key issues in biology - MODELIST - - EC:FP7:ERC2009-06-01 - 2015-05-31 - 233348 - VALID, and Bacterial, cellular and epigenetic factors that control enteropathogenicity - BacCellEpi - - H20202015-10-01 - 2018-09-30 - 670823 - VALID
Subjects: Cell Membrane Permeability, [SDV]Life Sciences [q-bio], Bacterial Toxins, MESH: Proteolysis, MESH: Hep G2 Cells, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, MESH: Heat-Shock Proteins, MESH: Listeria monocytogenes, Hemolysin Proteins, Mice, Animals, Humans, MESH: RAW 264.7 Cells, Listeriosis, MESH: Animals, MESH: Cell Membrane Permeability, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, MESH: Mice, Research Articles, Heat-Shock Proteins, MESH: Humans, Epithelial Cells, Hep G2 Cells, Listeria monocytogenes, [SDV] Life Sciences [q-bio], RAW 264.7 Cells, MESH: Bacterial Toxins, MESH: Hemolysin Proteins, MESH: Listeriosis, MESH: Epithelial Cells, Proteolysis, MESH: HeLa Cells, MESH: Caco-2 Cells, Caco-2 Cells, Lysosomes, Research Article, HeLa Cells, MESH: Lysosomes
Abstract: International audience; Bacterial pathogens can interfere during infection with host cell organelles, such as mitochondria, the endoplasmic reticulum-Golgi system or nuclei. As important cellular functions are often compartmentalized in these organelles, their targeting allows pathogens to manipulate key host functions during infection. Here, we identify lysosomes as a new class of organelles targeted by the pathogenic bacterium Listeria monocytogenes. We demonstrate that extracellular Listeria, via secretion of the pore-forming toxin listeriolysin O, alters lysosomal integrity in epithelial cells but not in macrophages. Listeriolysin O induces lysosomal membrane permeabilization and release of lysosomal content, such as cathepsins proteases, which remain transiently active in the host cytosol. We furthermore show that other bacterial pore-forming toxins, such as perfringolysin O and pneumolysin, also induce lysosomes alteration. Together, our data unveil a novel activity of bacterial cholesterol-dependent cytolysins.
Published: 2017
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14. 'Epidemic Clones' of Listeria monocytogenes Are Widespread and Ancient Clonal Groups

Author: Lise Frézal, Thierry Wirth, Laure Diancourt, Viviane Chenal-Francisque, Alexandre Leclercq, Marc Lecuit, Sylvain Brisse, Thomas Cantinelli, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Biologie des Infections - Biology of Infection, Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Institut Pasteur [Paris], Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL), Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Génomique évolutive des Microbes / Microbial Evolutionary Genomics, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris] (IP), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École Pratique des Hautes Études (EPHE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), DIAKITE, andrée, and Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS)
Subjects: Microbiology (medical), Genotype, [SDV]Life Sciences [q-bio], Biology, MESH: Listeria monocytogenes, MESH: Genotype, 03 medical and health sciences, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Phylogenetics, Genetic variation, Pulsed-field gel electrophoresis, Cluster Analysis, Humans, MESH: Molecular Epidemiology, Listeriosis, MESH: Genetic Variation, Typing, Epidemics, MESH: Phylogeny, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Phylogeny, MESH: Epidemics, 030304 developmental biology, Genetics, Molecular Epidemiology, 0303 health sciences, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, MESH: Humans, Molecular epidemiology, Phylogenetic tree, 030306 microbiology, Genetic heterogeneity, Genetic Variation, Bacteriology, Listeria monocytogenes, MESH: Cluster Analysis, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Electrophoresis, Gel, Pulsed-Field, [SDV] Life Sciences [q-bio], MESH: Electrophoresis, Gel, Pulsed-Field, MESH: Multilocus Sequence Typing, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Listeriosis, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Multilocus sequence typing, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Multilocus Sequence Typing
Abstract: The food-borne pathogen Listeria monocytogenes is genetically heterogeneous. Although some clonal groups have been implicated in multiple outbreaks, there is currently no consensus on how “epidemic clones” should be defined. The objectives of this work were to compare the patterns of sequence diversity on two sets of genes that have been widely used to define L. monocytogenes clonal groups: multilocus sequence typing (MLST) and multi-virulence-locus sequence typing (MvLST). Further, we evaluated the diversity within clonal groups by pulsed-field gel electrophoresis (PFGE). Based on 125 isolates of diverse temporal, geographical, and source origins, MLST and MvLST genes (i) had similar patterns of sequence polymorphisms, recombination, and selection, (ii) provided concordant phylogenetic clustering, and (iii) had similar discriminatory power, which was not improved when we combined both data sets. Inclusion of representative strains of previous outbreaks demonstrated the correspondence of epidemic clones with previously recognized MLST clonal complexes. PFGE analysis demonstrated heterogeneity within major clones, most of which were isolated decades before their involvement in outbreaks. We conclude that the “epidemic clone” denominations represent a redundant but largely incomplete nomenclature system for MLST-defined clones, which must be regarded as successful genetic groups that are widely distributed across time and space.
Published: 2013
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15. Splenic CD8α+ dendritic cells undergo rapid programming by cytosolic bacteria and inflammation to induce protective CD8+ T-cell memory

Author: Saidi M.Homa Soudja, Grégoire Lauvau, Laura Campisi, Nicolas Glaichenhaus, Frédéric Brau, Anne Lazzari, Frederic Geissmann, Emilie Narni-Mancinelli, Julie Cazareth, Delphine Bassand, Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Centre for Cellular and Molecular Biology of Inflammation, King‘s College London, Institut de pharmacologie moléculaire et cellulaire (IPMC), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Department of Microbiology and Immunology, and Albert Einstein College of Medicine [New York]
Subjects: MESH: Inflammation, Adoptive cell transfer, MESH: Spleen, Priming (immunology), Cell Count, MESH: T-Lymphocyte Subsets, MESH: Virulence, CD8-Positive T-Lymphocytes, MESH: Listeria monocytogenes, Lymphocyte Activation, MESH: Membrane Transport Proteins, Mice, Cytosol, 0302 clinical medicine, MESH: Cytosol, T-Lymphocyte Subsets, Immunology and Allergy, Cytotoxic T cell, MESH: Animals, Listeriosis, MESH: Bacterial Proteins, Cells, Cultured, Sequence Deletion, Adenosine Triphosphatases, Mice, Inbred BALB C, 0303 health sciences, MESH: Dendritic Cells, Virulence, biology, MESH: Sequence Deletion, Acquired immune system, MESH: CD8-Positive T-Lymphocytes, Adoptive Transfer, 3. Good health, MESH: Immunologic Memory, [SDV.IMM]Life Sciences [q-bio]/Immunology, medicine.symptom, MESH: Cells, Cultured, CD8 Antigens, Immunology, MESH: Mice, Inbred BALB C, Inflammation, 03 medical and health sciences, Bacterial Proteins, Antigen, MHC class I, MESH: Adenosine Triphosphatases, medicine, Animals, MESH: Lymphocyte Activation, MESH: Mice, 030304 developmental biology, SecA Proteins, MESH: Cell Count, Membrane Transport Proteins, Dendritic Cells, Listeria monocytogenes, MESH: Adoptive Transfer, MESH: Listeriosis, biology.protein, MESH: Antigens, CD8, Immunologic Memory, SEC Translocation Channels, Spleen, CD8, 030215 immunology
Abstract: International audience; Memory CD8(+) T lymphocytes are critical effector cells of the adaptive immune system mediating long-lived pathogen-specific protective immunity. Three signals - antigen, costimulation and inflammation - orchestrate optimal CD8(+) T-cell priming and differentiation into effector and memory cells and shape T-cell functional fate and ability to protect against challenge infections. While among the conventional spleen DCs (cDCs), the CD8α(+) but not the CD8α(-) cDCs most efficiently mediate CD8(+) T-cell priming, it is unclear which subset, irrespective of their capacity to process MHC class I-associated antigens, is most efficient at inducing naïve CD8(+) T-cell differentiation into pathogen-specific protective memory cells in vivo. Moreover, the origin of the required signals is still unclear. Using mice infected with the intracellular bacterium Listeria monocytogenes, we show that splenic CD8α(+) cDCs become endowed with all functional features to optimally prime protective memory CD8(+) T cells in vivo within only a few hours post-immunization. Such programming requires both cytosolic signals resulting from bacterial invasion of the host cells and extracellular inflammatory mediators. Thus, these data designate these cells as the best candidates to facilitate the development of cell-based vaccine therapy.
Published: 2011
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16. Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

Author: Sylvain Brisse, Marie Touchon, Morgane Lavina, Mylène M. Maury, Marc Lecuit, Viviane Chenal-Francisque, Alexandre Leclercq, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Cellule Pasteur, Université Paris Diderot - Paris 7 (UPD7)-PRES Sorbonne Paris Cité, Génomique évolutive des Microbes / Microbial Evolutionary Genomics, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Biologie des Infections - Biology of Infection, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), This work was supported financially by the Institut Pasteur, INSERM, French Government Investissement d'Avenir grant ANR-10-LABX-62-IBEID, and Institut de Veille Sanitair, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Centre National de Référence Listeria - Biologie des Infections (CNRL), Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), PRES Sorbonne Paris Cité-Université Paris Diderot - Paris 7 (UPD7), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris], Institut des Maladies Génétiques Imagine [Paris], and ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010)
Subjects: Microbiology (medical), Time Factors, Genotype, Epidemiology, Biology, medicine.disease_cause, MESH: Listeria monocytogenes, Microbiology, MESH: Genotype, Listeria monocytogenes, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Multiplex polymerase chain reaction, medicine, Humans, Listeriosis, MESH: Food Microbiology, MESH: Humans, MESH: Molecular Typing, MESH: Multiplex Polymerase Chain Reaction, MESH: Time Factors, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 3. Good health, Molecular Typing, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Listeriosis, Food Microbiology, Multilocus sequence typing, Identification (biology), Multiplex Polymerase Chain Reaction, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones.
Published: 2015
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17. Targeting and crossing of the human maternofetal barrier byListeria monocytogenes: Role of internalin interaction with trophoblast E-cadherin

Author: Michel Huerre, Marc Lecuit, Marie-Cécile Vacher-Lavenu, Jeffrey I. Gordon, D. Michael Nelson, Pascale Cossart, Huot Khun, Steven D. Smith, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Washington University School of Medicine in St. Louis, Washington University in Saint Louis (WUSTL), Histotechnologie et Pathologie, Institut Pasteur [Paris] (IP), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was supported in part by grants from the Pasteur Institute (to P.C. and M.H.) and National Institutes of Health Grants HD29190 (to D.M.N.) and DK30292 (to J.I.G.). M.L. was the recipient of a European Molecular Biology Organization postdoctoral fellowship. P.C. is an International Research Scholar of the Howard Hughes Medical Institute., Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris], Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Washington University in St Louis, CHU Cochin [AP-HP], and DIAKITE, andrée
Subjects: listeria, MESH: Trophoblasts, Placenta, [SDV]Life Sciences [q-bio], MESH: Maternal-Fetal Exchange, Syncytiotrophoblasts, MESH: Listeria monocytogenes, medicine.disease_cause, MESH: Cadherins, MESH: Pregnancy, Pregnancy, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Listeriosis, Pregnancy Complications, Infectious, Maternal-Fetal Exchange, MESH: Bacterial Proteins, bactérie, 0303 health sciences, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Multidisciplinary, LISTERIA MONOCYTOGENES, FETOPLACENTAL LISTERIOSIS, Biological Sciences, Cadherins, MESH: Placenta, Trophoblasts, 3. Good health, Cell biology, [SDV] Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, medicine.anatomical_structure, embryonic structures, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Female, bactérie pathogène, Protein Binding, Secondary infection, Biology, 03 medical and health sciences, Syncytiotrophoblast, Bacterial Proteins, Listeria monocytogenes, E-CADHERIN, medicine, Humans, MESH: Protein Binding, MESH: Pregnancy Complications, Infectious, intéraction, Internalin, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, 030304 developmental biology, MESH: Humans, 030306 microbiology, INTERNALIN, Trophoblast, Apical membrane, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Virology, MESH: Listeriosis, MICROBIAL INFECTION STRATEGY, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Listeria monocytogenesproduces severe fetoplacental infections in humans. How it targets and crosses the maternofetal barrier is unknown. We used immunohistochemistry to examine the location ofL. monocytogenesin placental and amniotic tissue samples obtained from women with fetoplacental listeriosis. The results raised the possibility thatL. monocytogenescrosses the maternofetal barrier through the villous syncytiotrophoblast, with secondary infection occurring via the amniotic epithelium. Because epidemiological studies indicate that the bacterial surface protein, internalin (InlA), may play a role in human fetoplacental listeriosis, we investigated the cellular patterns of expression of its host receptor, E-cadherin, at the maternofetal interface. E-cadherin was found on the basal and apical plasma membranes of syncytiotrophoblasts and in villous cytotrophoblasts. Established trophoblastic cell lines, primary trophoblast cultures, and placental villous explants were each exposed to isogenic InlA+ or InlA- strains ofL. monocytogenes,and toL. innocuaexpressing or not InlA. Quantitative assays of cellular invasion demonstrated that bacterial entry into syncytiotrophoblasts occurs via the apical membrane in an InlA–E-cadherin dependent manner. In human placental villous explants, bacterial invasion of the syncytiotrophoblast barrier and underlying villous tissue and subsequent replication produces histopathological lesions that mimic those seen in placentas of women with listeriosis. Thus, the InlA–E-cadherin interaction that plays a key role in the crossing of the intestinal barrier in humans is also exploited byL. monocytogenesto target and cross the placental barrier. Such a ligand–receptor interaction allowing a pathogen to specifically cross the placental villous trophoblast barrier has not been reported previously.
Published: 2004
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18. GATA-3 promotes maturation, IFN-y production, and liver-specific homing of nk cells

Author: Sandrine I. Samson, Thomas Ranson, Jan Buer, Odile Richard, Frank Grosveld, Christian A. J. Vosshenrich, Isabelle Godin, Francesco Colucci, Manuela Tavian, James P. Di Santo, Cytokines et Développement Lymphoïde, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Ontogenese de l'Hematopoiese, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Gesellschaft für Biotechnologische Forschung (GBF), Erasmus University Rotterdam, Hematopoïèse et Cellules Souches (U362), Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Supported by grants from the Pasteur Institute, Institut National de la Santé et la Recherche Medicale (Inserm), the Association pour le Recherche contre le Cancer (ARC), and the ACI Biologie du Développement. S.I.S. was supported by a fellowship from the MENRT, the Ligue National Contre le Cancer, and the ARC. C.A.J.V. was supported by a Poste Vert fellowship from the Inserm., Cell biology, and Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: MESH: Cell Differentiation, MESH: Killer Cells, Natural, MESH: Trans-Activators, MESH: Interferon-gamma, T cell, [SDV]Life Sciences [q-bio], Immunology, GATA3 Transcription Factor, Biology, CD49b, 03 medical and health sciences, Interleukin 21, Interferon-gamma, Mice, 0302 clinical medicine, Cell Movement, MESH: GATA3 Transcription Factor, medicine, Animals, Immunology and Allergy, Listeriosis, MESH: Animals, Antigen-presenting cell, MESH: Mice, MESH: Cell Movement, MESH: Chimera, 030304 developmental biology, 0303 health sciences, Lymphokine-activated killer cell, Chimera, Janus kinase 3, Cell Differentiation, Cell biology, DNA-Binding Proteins, Killer Cells, Natural, medicine.anatomical_structure, Infectious Diseases, Liver, MESH: Listeriosis, embryonic structures, Interleukin 12, Myeloid-derived Suppressor Cell, Trans-Activators, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: DNA-Binding Proteins, 030215 immunology, MESH: Liver
Abstract: International audience; The GATA-3 transcription factor has a determinant role in T cell specification and is an essential mediator of T helper 2-type polarized immune responses. While both committed NK precursors and mature NK cells express GATA-3, a role of this transcription factor in murine NK cell differentiation is not known. We found that NK cells, in contrast to T cells, can be generated in the absence of GATA-3. However, while GATA-3 antagonizes IFN-gamma production in differentiating T cells, GATA-3-deficient NK cells paradoxically produced less IFN-gamma compared to control NK cells and failed to provide early protection in vivo against infection with Listeria monocytogenes. Surprisingly, GATA-3 was essential for NK cell homing to the liver. Our results suggest that GATA-3 promotes NK cell maturation and acts in this lineage to specify distinct effector phenotypes.
Published: 2003
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19. Liver-Resident Macrophage Necroptosis Orchestrates Type 1 Microbicidal Inflammation and Type-2-Mediated Tissue Repair during Bacterial Infection

Author: Grégory Jouvion, Camille Blériot, Olivier Disson, Théo Dupuis, Marc Lecuit, Gérard Eberl, Biologie des Infections - Biology of Infection, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Histopathologie humaine et Modèles animaux, Institut Pasteur [Paris], Développement des Tissus Lymphoïdes, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), This work was financed by Institut Pasteur, Institut National de la Santé et de la Recherche Médicale, Institut Carnot, Fondation pour la Recherche Médicale, Ville de Paris, Fondation BNP Paribas, LabEx IBEID, the European Research Council, and Listress EU program., We thank the members of the Biology of Infection Unit, and in particular Y.H. Tsai. We thank G. Milon and S. Berrè for helpful discussions and critical reading of the manuscript. We thank H. Bierne for her contribution in the initial phase of the project and P. Cossart for providing the Lm Δhly mutant. We thank T. Walzer at ENS Lyon for Ccr2−/− mice, Ph. Bousso and F. Montalvao for MaFIA-GFP mice and helpful discussions, M. Leite de Moraes for Il4−/− mice, B. Ryffel for Il1rl1−/− mice, V. Dixit for Rip3−/− mice, T. Taniguchi for Irf3−/− mice, R. Menard for Flk1-gfp mice, and M. Albert and N. Yatim for Casp1−/− and Ifnar−/− mice and helpful discussions. We thank S. Novault of the PFC at Institut Pasteur and N. Sérafini for their help in the FACS experiments., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 116472,FCT::,ERA-PTG/2010,ERA-PTG/0003/2010(2011), Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris], Institut des Maladies Génétiques Imagine [Paris], ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)
Subjects: MESH: Inflammation, Necrosis, Cellular differentiation, [SDV]Life Sciences [q-bio], Complement Pathway, Alternative, MESH: Listeria monocytogenes, MESH: Monocytes, Monocytes, Mice, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Macrophage, Immunology and Allergy, Homeostasis, Listeriosis, MESH: Animals, MESH: Phagocytosis, Cells, Cultured, 0303 health sciences, Kupffer cell, Cell Differentiation, medicine.anatomical_structure, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Liver, MESH: Homeostasis, [SDV.IMM]Life Sciences [q-bio]/Immunology, medicine.symptom, MESH: Cells, Cultured, MESH: Cell Differentiation, MESH: Interleukins, MESH: Interleukin-33, Kupffer Cells, Necroptosis, Immunology, Inflammation, Mice, Inbred Strains, Biology, MESH: Mice, Inbred Strains, 03 medical and health sciences, Immune system, Phagocytosis, medicine, Animals, MESH: Mice, 030304 developmental biology, MESH: Necrosis, Wound Healing, Monocyte, Interleukins, MESH: Interleukin-4, Interleukin-33, Listeria monocytogenes, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Wound Healing, MESH: Kupffer Cells, MESH: Listeriosis, Interleukin-4, MESH: Complement Pathway, Alternative, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030215 immunology, MESH: Liver
Abstract: International audience; Kupffer cells, the phagocytes of fetal origin that line the liver sinusoids, are key contributors of host defense against enteroinvasive bacteria. Here, we found that infection by Listeria monocytogenes induced the early necroptotic death of Kupffer cells, which was followed by monocyte recruitment and an anti-bacterial type 1 inflammatory response. Kupffer cell death also triggered a type 2 response that involved the hepatocyte-derived alarmin interleukin-33 (IL-33) and basophil-derived interleukin-4 (IL-4). This led to the alternative activation of the monocyte-derived macrophages recruited to the liver, which thereby replaced ablated Kupffer cells and restored liver homeostasis. Kupffer cell death is therefore a key signal orchestrating type 1 microbicidal inflammation and type-2-mediated liver repair upon infection. This indicates that beyond the classical dichotomy of type 1 and type 2 responses, these responses can develop sequentially in the context of a bacterial infection and act interdependently, orchestrating liver immune responses and return to homeostasis, respectively.
Published: 2015
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20. Worldwide Distribution of Major Clones of Listeria monocytogenes

Author: Thomas Cantinelli, Coralie Tran, Viviane Chenal-Francisque, Marc Lecuit, Sylvain Brisse, Jodie Lopez, Alexandre Leclercq, Valérie Caro, Institut Pasteur [Paris], Laboratoire des Listeria, Centre National de Référence des Listeria, et Centre collaborateur de l’OMS pour la listériose d’origine alimentaire (CNR - CCOMS), and Institut Pasteur [Paris] (IP)
Subjects: Serotype, [SDV]Life Sciences [q-bio], lcsh:Medicine, MESH: Genome, Bacterial, MESH: Listeria monocytogenes, medicine.disease_cause, phylogeny, Listeriosis, MESH: Animals, bacteria, MESH: Phylogeny, Genetics, 0303 health sciences, MESH: Molecular Typing, Dispatch, genetic diversity, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, epidemiology, Public Health, MESH: Public Health, Microbiology (medical), Virulence, Biology, Microbiology, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, Listeria monocytogenes, geographic distribution, medicine, Animals, Humans, lcsh:RC109-216, Typing, Serotyping, Genotyping, 030304 developmental biology, MESH: Humans, 030306 microbiology, Genetic heterogeneity, MESH: Clone Cells, lcsh:R, typing, MESH: Serotyping, Outbreak, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Clone Cells, Molecular Typing, MESH: Listeriosis, Multilocus sequence typing, Genome, Bacterial, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Listeria monocytogenes is a foodborne pathogen that can cause listeriosis, a severe invasive infection in humans with a particularly high case-fatality rate. Listeriosis is a major public health concern in all world regions, with an increasing incidence in Europe, especially among elderly persons (1,2). L. monocytogenes is genetically heterogeneous (3–5). To help epidemiologic investigation and to define clones, i.e., groups of genetically similar isolates descending from a common ancestor, a variety of typing methods have been used, including pulsed-field gel electrophoresis (5,6), single nucleotide polymorphism typing (7), and multiple housekeeping and virulence gene sequencing (8,9). Some clones implicated in multiple outbreaks have been defined as epidemic clones (EC) (3,5,9–11). ECI and ECIV have been described in several countries (3,5), but because of the lack of standardization of genotyping, a definition of clones is not widely accepted, and current knowledge on the global distribution of L. monocytogenes clones is virtually absent. Multilocus sequence typing (MLST) is a reference method for global epidemiology and population biology of bacteria, and its application to L. monocytogenes (12) effectively allows isolate comparisons across laboratories (www.pasteur.fr/mlst). The aim of this study was to investigate the global distribution of L. monocytogenes MLST-defined clones.
Published: 2011
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21. Global burden of listeriosis: the tip of the iceberg

Author: Caroline Charlier, Alexandre Leclercq, Marc Lecuit, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Biologie des Infections - Biology of Infection, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Centre National de Référence Listeria - Biologie des Infections (CNRL), Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris], and Institut des Maladies Génétiques Imagine [Paris]
Subjects: Male, MESH: Humans, [SDV]Life Sciences [q-bio], Biology, MESH: Listeria monocytogenes, Listeria monocytogenes, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Article, Iceberg, MESH: Male, 3. Good health, Infectious Diseases, Oceanography, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Listeriosis, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Humans, Female, Listeriosis, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Comment on The global burden of listeriosis: a systematic review and meta-analysis. [Lancet Infect Dis. 2014]; International audience; Comment
Published: 2014
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22. A trip in the 'New Microbiology' with the bacterial pathogen Listeria monocytogenes

Author: Alice Lebreton, Pascale Cossart, Interactions Bactéries-Cellules (UIBC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris)-École normale supérieure - Paris (ENS Paris)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), financial support from Pasteur Institute, INSERM and INRA, from the European Research Council (ERC) advanced Grant 233348, from Agence Nationale de la Recherche (ANR BacNet 10-BINF-0201 and EpiLis 11-BSV3-003-01), from Laboratoires d’Excellence (IBEID ANR-10-LABX-62-01), from Fondation Jeantet and Fondation le Roch Les Mousquetaires., ANR: 10-BINF-02-01,BACNET,ANR, ANR-11-BSV3-0003,EPILIS,Reprogrammation épigénétique par la bactérie pathogène Listeria monocytogenes(2011), ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de biologie de l'ENS Paris (IBENS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANR-10-BINF-0002,BACNET,Exploring composition and dynamics of bacterial regulatory networks(2010), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 233348,EC:FP7:ERC,ERC-2008-AdG,MODELIST(2009), Institut National de la Recherche Agronomique (INRA) - Institut Pasteur [Paris] - Institut National de la Santé et de la Recherche Médicale (INSERM), ANR : 10-BINF-02-01, BACNET, ANR, ANR-11-BSV3-0003, EPILIS, Reprogrammation épigénétique par la bactérie pathogène Listeria monocytogenes(2011), ANR-10-LABX-62-IBEID, IBEID, Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), European Project : 233348, MODELIST, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Lebreton, Alice, Exploring composition and dynamics of bacterial regulatory networks - - BACNET2010 - ANR-10-BINF-0002 - BINF - VALID, BLANC - Reprogrammation épigénétique par la bactérie pathogène Listeria monocytogenes - - EPILIS2011 - ANR-11-BSV3-0003 - BLANC - VALID, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Understanding the infection by the bacterium Listeria monocytogenes as a way to address key issues in biology - MODELIST - - EC:FP7:ERC2009-06-01 - 2015-05-31 - 233348 - VALID, and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris
Subjects: Transcription, Genetic, Cellular microbiology, medicine.disease_cause, MESH: Listeria monocytogenes, Biochemistry, Epigenesis, Genetic, Structural Biology, Listeriosis, MESH: Animals, MESH: Epigenesis, Genetic, Pathogen, 0303 health sciences, MESH: Gene Expression Regulation, Bacterial, biology, Non-coding RNA, Endocytosis, Chromatin, Host-Pathogen Interactions, Intracellular, Biophysics, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Clathrin, Microbiology, 03 medical and health sciences, Listeria monocytogenes, Genetics, medicine, Animals, Humans, Molecular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, 030304 developmental biology, MESH: Humans, 030306 microbiology, MESH: Transcription, Genetic, MESH: Host-Pathogen Interactions, MESH: Chromatin Assembly and Disassembly, Cell Biology, Gene Expression Regulation, Bacterial, biology.organism_classification, Chromatin Assembly and Disassembly, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Listeriosis, biology.protein, Listeria, Mitochondrial dynamics, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Abstract: International audience; Listeria monocytogenes is a food-borne pathogen causing an opportunistic disease called listeriosis. This bacterium invades and replicates in most cell types, due to its multiple strategies to exploit host molecular mechanisms. Research aiming at unravelling Listeria invasion and intracellular lifestyle has led to a number of key discoveries in infection biology, cell biology and also microbiology. In this review, we report on our most recent advances in understanding the intimate crosstalk between the bacterium and its host, resulting from in-depth studies performed over the past five years. We specifically highlight new concepts in RNA-based regulation in bacteria and discuss important findings in cell biology, including a new role for clathrin and an atypical mitochondrial fragmentation mechanism. We also illustrate the notion that bacterial infection regulates host gene expression at the chromatin level, contributing to an emerging field called patho-epigenetics. This review corresponds to the lecture given by one of us (P.C.) on the occasion of the 2014 FEBS|EMBO Woman in Science Award.
Published: 2014
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23. Infections et grossesse : un danger pour la mère et l'enfant

Author: Charlier, Caroline, Lecuit, Marc, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Centre d'infectiologie Necker-Pasteur [CHU Necker], Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP], Biologie des Infections - Biology of Infection, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris], and Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS)
Subjects: MESH: Humans, [SDV]Life Sciences [q-bio], MESH: Rubella, MESH: Rubella Syndrome, Congenital, MESH: Vaccination, MESH: Cytomegalovirus Infections, MESH: HIV Infections, MESH: Streptococcus agalactiae, MESH: Male, MESH: HIV-1, MESH: Infectious Disease Transmission, Vertical, MESH: Pregnancy, MESH: Streptococcal Infections, MESH: Listeriosis, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Chickenpox, MESH: Pregnancy Complications, Infectious, MESH: Contraindications, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Chaque année, on dénombre plus de 800 000 grossesses en France. Il n'est pas rare au cours de cette période qu'une femme enceinte soit confrontée à un événement évoquant une infection : fièvre, contage infectieux, découverte d'anomalies sérologiques lors de dépistages systématiques, prescription de traitements antibiotiques, administration d'un vaccin sont autant de situations fréquentes, dont le clinicien doit avoir la maîtrise chez la femme enceinte. Des enjeux multiples Les enjeux d'une infection maternelle sont multiples et souvent chevauchants. Le premier est maternel, car certaines infections sont démontrées plus sévères chez la femme enceinte.
Published: 2014
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24. Highly Rifampin-Resistant Listeria monocytogenes Isolated from a Patient with Prosthetic Bone Infection

Author: Patrice Courvalin, Marc Lecuit, Hélène Dieye, Viviane Chenal-Francisque, Saleem Mehvish, Caroline Charlier, Alexandre Leclercq, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Biologie des Infections - Biology of Infection, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Agents antibactériens, Institut Pasteur [Paris] (IP), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris], and DIAKITE, andrée
Subjects: [SDV]Life Sciences [q-bio], Antibiotics, medicine.disease_cause, MESH: Listeria monocytogenes, chemistry.chemical_compound, Moxifloxacin, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, polycyclic compounds, Medicine, heterocyclic compounds, Pharmacology (medical), Prosthesis-Related Infection, ComputingMilieux_MISCELLANEOUS, MESH: Aged, 0303 health sciences, MESH: Microbial Sensitivity Tests, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Glycopeptide, 3. Good health, [SDV] Life Sciences [q-bio], Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, medicine.drug, medicine.drug_class, Microbiology, Bone Infection, 03 medical and health sciences, Listeria monocytogenes, MESH: Anti-Bacterial Agents, MESH: Drug Resistance, Bacterial, Letters to the Editor, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, 030304 developmental biology, Pharmacology, MESH: Humans, 030306 microbiology, business.industry, MESH: Prosthesis-Related Infections, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Virology, MESH: Rifampin, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, chemistry, MESH: Listeriosis, Linezolid, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Listeriosis is a rare but severe food-borne infection due to Listeria monocytogenes. L. monocytogenes is naturally susceptible to a wide range of antibiotics, including penicillins, aminoglycosides, tetracyclines, macrolides, co-trimoxazole, linezolid, moxifloxacin, glycopeptides, and rifampin, but
Published: 2014
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25. Listeria monocytogenes-Associated Biliary Tract Infections

Author: Charlier, Caroline, Fevre, Cindy, Travier, Laetitia, Cazenave, Benoît, Bracq-Dieye, Hélène, Podevin, Juliette, Assomany, Daher, Guilbert, Lydie, Bossard, Céline, Carpentier, Françoise, Cales, Valérie, Leclercq, Alexandre, Lecuit, Marc, Centre National de Référence Listeria - Biologie des Infections (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie des Infections - Biology of Infection, Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), Centre d'infectiologie Necker-Pasteur [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Centre hospitalier universitaire de Nantes (CHU Nantes), Centre Hospitalier de Roubaix, Centre hospitalier de Pau, Université Paris Descartes - Paris 5 (UPD5), This study received financial support form Institut Pasteur, Inserm, Fondation BNP Paribas, Ville de Paris, Fondation pour la Recherche Médicale and Institut de Veille Sanitaire., Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), and Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP]
Subjects: MESH: Cholecystectomy, [SDV]Life Sciences [q-bio], MESH: Comorbidity, MESH: Medication Errors, MESH: Listeria monocytogenes, MESH: Population Surveillance, MESH: Aged, 80 and over, MESH: Penicillins, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Anti-Bacterial Agents, MESH: Drug Resistance, Bacterial, MESH: Fever, MESH: Cholangitis, MESH: Bacteremia, MESH: Cholecystitis, MESH: Aged, MESH: Necrosis, MESH: Humans, MESH: Middle Aged, MESH: Biliary Tract Diseases, MESH: Adult, MESH: Retrospective Studies, MESH: Gallbladder Diseases, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, MESH: Abdominal Pain, MESH: France, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Listeriosis, MESH: Immunosuppressive Agents, MESH: Cholecystolithiasis, MESH: Cysts, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; At present, little is known regarding Listeria mono-cytogenes-associated biliary tract infection, a rare form of listeriosis. In this article, we will study 12 culture-proven cases reported to the French National Reference Center for Listeria from 1996 to 2013 and review the 8 previously published cases. Twenty cases were studied: 17 cholecystitis, 2 cholangitis, and 1 biliary cyst infection. Half were men with a median age of 69 years (32-85). Comorbidities were present in 80%, including cirrhosis, rheumatoid arthritis, and diabetes. Five patients received immunosuppressive therapy, including cortico-steroids and anti-tumor necrosis factor biotherapies. Half were afebrile. Blood cultures were positive in 60% (3/5). Gallbladder histological lesions were analyzed in 3 patients and evidenced acute, chronic, or necrotic exacerbation of chronic infection. Genoserogroup of the 12 available strains were IVb (n ¼ 6), IIb (n ¼ 5), and IIa (n ¼ 1). Their survival in the bile was not enhanced when compared with isolates from other listeriosis cases. Adverse outcome was reported in 33% (5/15): 3 deaths, 1 recurrence; 75% of the patients with adverse outcome received inadequate antimicrobial therapy (P ¼ 0.033). Biliary tract listeriosis is a severe infection associated with high mortality in patients not treated with appropriate therapy. This study provides medical relevance to in vitro and animal studies that had shown Listeria monocytogenes ability to survive in bile and induce overt biliary infections. (Medicine 93(18):e105) Abbreviations: BHI = brain heart infusion, CNS = central nervous system, Lm = Listeria monocytogenes, MIC = minimal inhibitory concentration, MLST = multilocus sequence typing, MN = maternal-neonatal, NRCL = National Reference Center for Listeria, S = septicemia.
Published: 2014
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26. Internalin of Listeria monocytogenes with an intact leucine-rich repeat region is sufficient to promote internalization

Author: Hélène Ohayon, J Mengaud, Pascale Cossart, Laurence Braun, Marc Lecuit, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Cell type, [SDV]Life Sciences [q-bio], media_common.quotation_subject, Immunology, Leucine-rich repeat, Biology, MESH: Listeria monocytogenes, Microbiology, Bacterial Adhesion, 03 medical and health sciences, Bacterial Proteins, Leucine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Humans, Listeriosis, Internalin, MESH: Bacterial Adhesion, Internalization, MESH: Bacterial Proteins, Repetitive Sequences, Nucleic Acid, 030304 developmental biology, media_common, 0303 health sciences, MESH: Humans, MESH: Repetitive Sequences, Nucleic Acid, 030306 microbiology, Cell adhesion molecule, Transfection, Listeria monocytogenes, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, In vitro, Cell biology, MESH: Leucine, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, MESH: Listeriosis, Cell culture, Parasitology, MESH: Caco-2 Cells, Caco-2 Cells, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Research Article
Abstract: International audience; Listeria monocytogenes can use two different surface proteins, internalin (InlA) and InlB, to invade mammalian cells. The exact role of these invasiveness factors in vivo remains to be determined. In cultured cells, InlA is necessary to promote Listeria entry into human epithelial cells, such as Caco-2 cells, whereas InlB is necessary to promote Listeria internalization in several other cell types, including hepatocytes, fibroblasts, and epithelioid cells, such as Vero, HeLa, CHO, or Hep-2 cells. We have recently reported that the InlA receptor on Caco-2 cells is the cell adhesion molecule E-cadherin and demonstrated that nonpermissive fibroblasts become permissive for internalin-mediated entry when transfected with the gene coding for LCAM, the chicken homolog of the human E-cadherin gene. In this study, we demonstrate for the first time that the internalin protein alone is sufficient to promote internalization into cells expressing its receptor. Indeed, internalin confers invasiveness to both Enterococcus faecalis and internalin-coated latex beads. As shown by transmission electron microscopy, these beads were phagocytosed via a "zipper" mechanism similar to that observed during the internalin-E-cadherin-mediated entry of Listeria. Moreover, a functional analysis of internalin demonstrates that its amino-terminal region, encompassing the leucine-rich repeat (LRR) region and the inter-repeat (IR) region, is necessary and sufficient to promote bacterial entry into cells expressing its receptor. Several lines of evidence suggest that the LRR region would interact directly with E-cadherin, whereas the IR region would be required for a proper folding of the LRR region.
Published: 1997
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27. A role for SIRT2-dependent histone H3K18 deacetylation in bacterial infection

Author: Guillaume Soubigou, Haig A. Eskandarian, Francis Impens, Pascale Cossart, Melanie Hamon, Marie-Anne Nahori, Jean-Yves Coppée, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Medical Protein Research (VIB), Vlaams Instituut voor Biotechnologie, Department of Biochemistry, Universiteit Gent = Ghent University (UGENT), Transcriptome et Epigénome (PF2), Institut Pasteur [Paris] (IP), Work in the Cossart laboratory is funded by the Pasteur Institute, Inserm (U604), Institut National de la Recherche Agronomique (USC2020), the Fondation Louis Jeantet, the European Research Council (advanced grant 233348 MODELIST), the Fondation Le Roch Les Mousquetaires, and the Agence Nationale pour la Recherche grants (ERANET 'LISTRESS' and EPILIS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Unité Sous Contrat 2020, Institut National de la Recherche Agronomique (INRA), Ghent University [Belgium] (UGENT), Institut Pasteur [Paris], Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Universiteit Gent = Ghent University [Belgium] (UGENT)
Subjects: NF-KAPPA-B, MESH: Proto-Oncogene Proteins c-met, medicine.disease_cause, MESH: Listeria monocytogenes, MESH: Mice, Knockout, Histones, Mice, 0302 clinical medicine, Cytosol, Sirtuin 2, MESH: Cytosol, Transcription (biology), MESH: Sirtuin 2, Listeriosis, MESH: Animals, LISTERIA-MONOCYTOGENES, PHOSPHORYLATION, MESH: Bacterial Proteins, GENE-EXPRESSION, Mice, Knockout, MESH: Histones, 0303 health sciences, Mice, Inbred BALB C, Multidisciplinary, IFN-GAMMA, biology, IMMUNE-RESPONSES, Proto-Oncogene Proteins c-met, Chromatin, 3. Good health, Histone, Sirtuin, Host-Pathogen Interactions, MESH: Transcription Initiation Site, MESH: Membrane Proteins, Transcription Initiation Site, MESH: Acetylation, Transcriptional Activation, MESH: Cell Nucleus, ACETYLATION, MESH: Mice, Inbred BALB C, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, SIRT2, Microbiology, MESH: Chromatin, 03 medical and health sciences, Histone H3, Listeria monocytogenes, Bacterial Proteins, medicine, Animals, Humans, MESH: Lysine, MESH: Mice, 030304 developmental biology, Cell Nucleus, MESH: Humans, Lysine, MESH: Host-Pathogen Interactions, Membrane Proteins, Acetylation, SIRTUINS, MESH: Listeriosis, MESH: Protein Processing, Post-Translational, MESH: HeLa Cells, biology.protein, PATTERNS, MESH: Transcriptional Activation, Protein Processing, Post-Translational, 030217 neurology & neurosurgery, HeLa Cells
Abstract: Bacterial Subversion Tactics Intracellular bacterial pathogens such as Listeria monocytogenes can change host cell transcription programs to promote infection. Eskandarian et al. ( 1238858 ) found that during infection, the Listeria effector protein InlB promoted the movement of a host protein deacetylase, SIRT2, from its normal location in the cytosol to the nucleus. In the nucleus, SIRT2 helped to repress a number of host cell genes by deacetylating one of their associated histones. In mice, reduced levels of SIRT2 impaired bacterial infection.
Published: 2013
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28. Atypical mitochondrial fission upon bacterial infection

Author: Richard J. Youle, Amy E. Palmer, Fabrizia Stavru, Pascale Cossart, Chunxin Wang, Institut National de la Recherche Agronomique (INRA), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Colorado [Boulder], National Institutes of Health [Bethesda] (NIH), Institut Pasteur Institut National de la Sant [604], Institut National de la Recherche Agronomique Unit, Nationale pour la Recherche, postdoctoral fellowships from European Molecular Biology Organization, recherche Medicale, Intramural Research Program of the National Institutes of Neurological Disorders and Stroke, National Institutes of Health, ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), National Institute of Neurological Disorders and Stroke, and echerche Medicale
Subjects: [SDV]Life Sciences [q-bio], Fluorescent Antibody Technique, Mitochondrion, MESH: Heat-Shock Proteins, Endoplasmic Reticulum, MESH: Listeria monocytogenes, GTP Phosphohydrolases, Hemolysin Proteins, 0302 clinical medicine, FUSION, MAMMALIAN-CELLS, Staurosporine, Listeriosis, LISTERIA-MONOCYTOGENES, MESH: Fluorescent Antibody Technique, Heat-Shock Proteins, 0303 health sciences, Multidisciplinary, MESH: Real-Time Polymerase Chain Reaction, Listeriolysin O, PROTEIN HFIS1, MESH: Mitochondrial Proteins, Biological Sciences, Cell biology, APOPTOSIS, [SDV] Life Sciences [q-bio], MESH: Hemolysin Proteins, Mitochondrial fission, Microtubule-Associated Proteins, actin, medicine.drug, Dynamins, endocrine system, MESH: GTP Phosphohydrolases, Bacterial Toxins, Blotting, Western, ENDOPLASMIC-RETICULUM, DRP1, Biology, Real-Time Polymerase Chain Reaction, Mitochondrial Proteins, 03 medical and health sciences, MESH: Endoplasmic Reticulum, medicine, Humans, MESH: Blotting, Western, Fragmentation (cell biology), PROTEOLYTIC CLEAVAGE, 030304 developmental biology, MESH: Humans, Endoplasmic reticulum, Listeria monocytogenes, Molecular biology, Fusion protein, mitochondrial dynamics, MESH: Mitochondrial Dynamics, MESH: Dynamins, live cell imaging, MESH: Microtubule-Associated Proteins, MESH: Bacterial Toxins, MESH: Listeriosis, MESH: HeLa Cells, DNAJA3, MORPHOLOGY, OUTER-MEMBRANE, 030217 neurology & neurosurgery, HeLa Cells
Abstract: International audience; We recently showed that infection by Listeria monocytogenes causes mitochondrial network fragmentation through the secreted pore-forming toxin listeriolysin O (LLO). Here, we examine factors involved in canonical fusion and fission. Strikingly, LLOinduced mitochondrial fragmentation does not require the traditional fission machinery, as Drp1 oligomers are absent from fragmented mitochondria following Listeria infection or LLO treatment, as the dynamin-like protein 1 (Drp1) receptor Mff is rapidly degraded, and as fragmentation proceeds efficiently in cells with impaired Drp1 function. LLO does not cause processing of the fusion protein optic atrophy protein 1 (Opa1), despite inducing a decrease in the mitochondrial membrane potential, suggesting a unique Drp1-and Opa1-independent fission mechanism distinct from that triggered by uncouplers or the apoptosis inducer staurosporine. We show that the ER marks LLO-induced mitochondrial fragmentation sites even in the absence of functional Drp1, demonstrating that the ER activity in regulating mitochondrial fission can be induced by exogenous agents and that the ER appears to regulate fission by a mechanism independent of the canonical mitochondrial fission machinery.
Published: 2013
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29. IL-22 modulates IL-17A production and controls inflammation and tissue damage in experimental dengue infection

Author: Guabiraba, Rodrigo, Besnard, Anne-Gaëlle, Marques, Rafael E, Maillet, Isabelle, Fagundes, Caio T, Conceição, Thais M, Rust, Naiara M, Charreau, Sandrine, Paris, Isabelle, Lecron, Jean-Claude, Renauld, Jean-Christophe, Quesniaux, Valérie, Da Poian, Andrea T, Arruda, Luciana B, Souza, Danielle G, Ryffel, Bernhard, Teixeira, Mauro M, Instituto de Ciências Biologicas (ICB), Universidade Federal de Minas Gerais, Immunologie et Neurogénétique Expérimentales et Moléculaires (INEM), Centre National de la Recherche Scientifique (CNRS)-Université d'Orléans (UO), Instituto de Bioquimica M'edica (IBqM), Universidade Federal do Rio de Janeiro (UFRJ), Instituto de Microbiologia Paulo de Góes (IMPG), Laboratoire Inflammation, Tissus épithéliaux et Cytokines (LITEC), Université de Poitiers, Ludwig Institute for Cancer Research, French National Research Agency (ANR), Fondation pour la Recherche Médicale (FRM), Fond Européen de Développement Régional (FEDER), Centre Nationale de la Recherche Scientifique (CNRS/France), le Studium (CNRS/France), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq/Brazil), and Fundaçao de Amparo a Pesquisa de Minas Gerais (FAPEMIG/Brazil)
Subjects: MESH: Inflammation, Male, MESH: CASP8 and FADD-Like Apoptosis Regulating Protein, Neutrophils, MESH: Interleukin-17, Apoptosis, MESH: Dengue, MESH: Protein Isoforms, MESH: Neutrophils, MESH: Dengue Virus, MESH: Receptors, Interleukin-17, MESH: Listeria monocytogenes, MESH: Mice, Knockout, Dengue, Mice, IL-17A, IL-22, MESH: Animals, Mice, Knockout, Receptors, Interleukin-17, Interleukin-17, Hep G2 Cells, Viral Load, MESH: Gene Expression Regulation, MESH: Antigens, CD95, Liver, MESH: HEK293 Cells, [SDV.IMM]Life Sciences [q-bio]/Immunology, Inflammation Mediators, MESH: Viral Load, MESH: Interleukins, MESH: Mice, Transgenic, MESH: Inflammation Mediators, MESH: Hep G2 Cells, MESH: Mice, Inbred C57BL, MESH: B-Lymphocytes, Animals, Humans, MESH: Lymphocyte Activation, MESH: Mice, MESH: Necrosis, Inflammation, MESH: Humans, Interleukin-6, MESH: Apoptosis, Interleukins, Dengue Virus, MESH: Interleukin-6, infection, MESH: Male, Mice, Inbred C57BL, Disease Models, Animal, MESH: T-Lymphocytes, Gene Expression Regulation, MESH: Listeriosis, MESH: Disease Models, Animal, MESH: Liver
Abstract: Guabiraba, Rodrigo Besnard, Anne-Gaelle Marques, Rafael E Maillet, Isabelle Fagundes, Caio T Conceicao, Thais M Rust, Naiara M Charreau, Sandrine Paris, Isabelle Lecron, Jean-Claude Renauld, Jean-Christophe Quesniaux, Valerie Da Poian, Andrea T Arruda, Luciana B Souza, Danielle G Ryffel, Bernhard Teixeira, Mauro M Germany Eur J Immunol. 2013 Jun;43(6):1529-44. doi: 10.1002/eji.201243229. Epub 2013 Apr 17.; International audience; Dengue virus (DENV), a mosquito-borne flavivirus, is a public health problem in many tropical countries. IL-22 and IL-17A are key cytokines in several infectious and inflammatory diseases. We have assessed the contribution of IL-22 and IL-17A in the pathogenesis of experimental dengue infection using a mouse-adapted DENV serotype 2 strain (P23085) that causes a disease that resembles severe dengue in humans. We show that IL-22 and IL-17A are produced upon DENV-2 infection in immune-competent mice. Infected IL-22(-/-) mice had increased lethality, neutrophil accumulation and pro-inflammatory cytokines in tissues, notably IL-17A. Viral load was increased in spleen and liver of infected IL-22(-/-) mice. There was also more severe liver injury, as seen by increased transaminases levels and tissue histopathology. γδ T cells and NK cells are sources of IL-17A and IL-22, respectively, in liver and spleen. We also show that DENV-infected HepG2 cells treated with rhIL-22 had reduced cell death and decreased IL-6 production. IL-17RA(-/-) mice were protected upon infection and IL-17A-neutralizing-Ab-treatment partially reversed the phenotype observed in IL-22(-/-) -infected mice. We suggest that disrupting the balance between IL-22 and IL-17A levels may represent an important strategy to reduce inflammation and tissue injury associated with severe dengue infection.
Published: 2012
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30. The intestinal microbiota interferes with the microRNA response upon oral Listeria infection

Author: Pierre Lechat, Odile Sismeiro, Alice Lebreton, Constance Ciaudo, Joern Toedling, Christophe Bécavin, Guillaume Soubigou, Cristel Archambaud, Pascale Cossart, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Transcriptome et Epigénome (PF2), Institut Pasteur [Paris] (IP), Mines Paris - PSL (École nationale supérieure des mines de Paris), Université Paris sciences et lettres (PSL), Génétique et Biologie du Développement, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Cancer et génome: Bioinformatique, biostatistiques et épidémiologie d'un système complexe, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Génopole, European Research Council (E.R.C.) Advanced grant [233348], ANR Investissement d'Avenir Programme [10-LABX-62], Institut Pasteur, Institut National de la Sante et de la Recherche Medicale, Fondation Jeantet and Fondation le Roch, Federation of European Biochemical Societies, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 233348,EC:FP7:ERC,ERC-2008-AdG,MODELIST(2009), Lebreton, Alice, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Understanding the infection by the bacterium Listeria monocytogenes as a way to address key issues in biology - MODELIST - - EC:FP7:ERC2009-06-01 - 2015-05-31 - 233348 - VALID, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Institut Pasteur [Paris], MINES ParisTech - École nationale supérieure des mines de Paris, Centre National de la Recherche Scientifique (CNRS)-Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Cancer et génôme: Bioinformatique, biostatistiques et épidémiologie d'un système complexe, MINES ParisTech - École nationale supérieure des mines de Paris-Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], ANR-10-LABX-0062/10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Subjects: Transcription, Genetic, Human pathogen, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], medicine.disease_cause, MOUSE, MESH: Listeria monocytogenes, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, IMMUNE-SYSTEM, MEDIATED REGULATION, MONOCYTOGENES, RECOGNITION, EXPRESSION, VIRULENCE, ESCAPE, CANCER, GUT, Transcriptome, Mice, 0302 clinical medicine, Listeriosis, MESH: Animals, Pathogen, 0303 health sciences, [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], biology, Microbiota, Life sciences, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], QR1-502, 3. Good health, 030220 oncology & carcinogenesis, Host-Pathogen Interactions, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Female, Research Article, Listeria infection, Microbiology, 03 medical and health sciences, MESH: Gene Expression Profiling, Immune system, Listeria monocytogenes, Ileum, MESH: Mice, Inbred C57BL, ddc:570, Virology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], microRNA, medicine, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Animals, MESH: Microbiota, MESH: Mice, 030304 developmental biology, Gene Expression Profiling, MESH: Transcription, Genetic, MESH: Host-Pathogen Interactions, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, biology.organism_classification, medicine.disease, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Mice, Inbred C57BL, MicroRNAs, MESH: Listeriosis, MESH: Ileum, Immunology, Listeria, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Female, MESH: MicroRNAs
Abstract: The intestinal tract is the largest reservoir of microbes in the human body. The intestinal microbiota is thought to be able to modulate alterations of the gut induced by enteropathogens, thereby maintaining homeostasis. Listeria monocytogenes is the agent of listeriosis, an infection transmitted to humans upon ingestion of contaminated food. Crossing of the intestinal barrier is a critical step of the infection before dissemination into deeper organs. Here, we investigated the role of the intestinal microbiota in the regulation of host protein-coding genes and microRNA (miRNA or miR) expression during Listeria infection. We first established the intestinal miRNA signatures corresponding to the 10 most highly expressed miRNAs in the murine ileum of conventional and germfree mice, noninfected and infected with Listeria. Next, we identified 6 miRNAs whose expression decreased upon Listeria infection in conventional mice. Strikingly, five of these miRNA expression variations (in miR-143, miR-148a, miR-200b, miR-200c, and miR-378) were dependent on the presence of the microbiota. In addition, as is already known, protein-coding genes were highly affected by infection in both conventional and germfree mice. By crossing bioinformatically the predicted targets of the miRNAs to our whole-genome transcriptomic data, we revealed an miRNA-mRNA network that suggested miRNA-mediated global regulation during intestinal infection. Other recent studies have revealed an miRNA response to either bacterial pathogens or commensal bacteria. In contrast, our work provides an unprecedented insight into the impact of the intestinal microbiota on host transcriptional reprogramming during infection by a human pathogen., IMPORTANCE While the crucial role of miRNAs in regulating the host response to bacterial infection is increasingly recognized, the involvement of the intestinal microbiota in the regulation of miRNA expression has not been explored in detail. Here, we investigated the impact of the intestinal microbiota on the regulation of protein-coding genes and miRNA expression in a host infected by L. monocytogenes, a food-borne pathogen. We show that the microbiota interferes with the microRNA response upon oral Listeria infection and identify several protein-coding target genes whose expression correlates inversely with that of the miRNA. Further investigations of the regulatory networks involving miR-143, miR-148a, miR-200b, miR-200c, and miR-378 will provide new insights into the impact of the intestinal microbiota on the host upon bacterial infection.
Published: 2012
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31. Bacteria tune interferon responses by playing with chromatin

Author: Hélène Bierne, Alice Lebreton, Pascale Cossart, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Ligue Nationale Contre le Cancer (LNCC RS10/75-76 Bierne), ANR-11-BSV3-0003,EPILIS,Reprogrammation épigénétique par la bactérie pathogène Listeria monocytogenes(2011), European Project: 233348,MODELIST, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Lebreton, Alice, BLANC - Reprogrammation épigénétique par la bactérie pathogène Listeria monocytogenes - - EPILIS2011 - ANR-11-BSV3-0003 - BLANC - VALID, ERC Advanced grant - MODELIST - 233348 - INCOMING, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA)
Subjects: Chromosomal Proteins, Non-Histone, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, medicine.disease_cause, MESH: Listeria monocytogenes, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Mice, Interferon, MESH: Animals, MESH: Interferons, Listeriosis, Pathogen, Chromatin, 3. Good health, Infectious Diseases, Host-Pathogen Interactions, medicine.drug, Microbiology (medical), BAHD1, Listeria, Immunology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, Microbiology, Mycobacterium, MESH: Chromatin, Listeria monocytogenes, MESH: Chromosomal Proteins, Non-Histone, medicine, Animals, Humans, Secretion, Epigenetics, chromatin complex, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, Gene, MESH: Mice, Innate immune system, MESH: Humans, epigenetics, MESH: Host-Pathogen Interactions, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Listeriosis, interferon-lambda, Parasitology, Interferons, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Abstract: International audience; Bacterial infections, like their viral counterparts, trigger the onset of innate immune defense mechanisms through the release of cytokines, including interferons (IFNs). While type I and II IFN responses to bacteria have long been explored, type III IFN response remains poorly addressed. We have recently reported that the pathogen Listeria monocytogenes triggers the expression of type I and III IFN genes in epithelial cells, and is able to fine-tune downstream signaling at the chromatin level. This bacterium can negatively or positively modulate the expression of interferon-stimulated genes (ISGs) by manipulating the function of BAHD1, a component of a host chromatin-silencing complex. To this end, L. monocytogenes tightly controls the secretion of a BAHD1 inhibitory factor, LntA. Here, we further document the current knowledge about chromatin mechanisms modulating interferon responses during host-bacteria interplay, and discuss their physiological consequences.
Published: 2012
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32. Tuning of natural killer cell reactivity by NKp46 and Helios calibrates T cell responses

Author: Bruce Beutler, Marta Gut, Sam Kung, Bernard Malissen, Claire Bernat, Simon Heath, Marie Malissen, Eric Vivier, Jordi Estellé, Frédéric Vély, Aude De Gassart, Louis Noël Gastinel, Emilie Narni-Mancinelli, Aurore Fenis, Sophie Ugolini, Baptiste N. Jaeger, Elodie Bertosio, Sajid Mahmood, Ivo Gut, Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Gustave Roussy (IGR), Vectorologie et transfert de gènes (VTG / UMR8121), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11), University of Manitoba [Winnipeg], Centre National de Génotypage (CNG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centro Nacional de Análisi Genómico (CNAG), Centro Nacional de Análisis Genómico, Service de Chirurgie, Assistance Publique - Hôpitaux de Marseille (APHM)-Hospices Civiles de Marseille-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Pharmacologie des Immunosuppresseurs et de la Transplantation (PIST), Université de Limoges (UNILIM)-CHU Limoges-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Immunology and Microbial Science, The Scripps Research Institute [La Jolla, San Diego], Centre Nacional D'Anàlisi Genómica, Parc Cientific de Barcelona, Apotex Center - University of Manitoba, Haon, Marie Laure, Institut National de la Santé et de la Recherche Médicale (INSERM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Université de Limoges (UNILIM)-CHU Limoges, Scripps Research Institute, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)
Subjects: Muromegalovirus, Transcription, Genetic, T-Lymphocytes, Cell, Mutant, MESH: Herpesviridae Infections, Adaptive Immunity, CD8-Positive T-Lymphocytes, medicine.disease_cause, MESH: Down-Regulation, MESH: Antibodies, Monoclonal, Mice, 0302 clinical medicine, Antigens, Ly, MESH: Animals, Listeriosis, MESH: Mutagenesis, 0303 health sciences, Mutation, MESH: Genetic Complementation Test, Multidisciplinary, Antibodies, Monoclonal, MESH: Antigens, Ly, MESH: Transcription Factors, Herpesviridae Infections, Viral Load, MESH: Amino Acid Substitution, MESH: CD8-Positive T-Lymphocytes, 3. Good health, Cell biology, DNA-Binding Proteins, Killer Cells, Natural, medicine.anatomical_structure, MESH: Immunologic Memory, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Viral Load, MESH: Killer Cells, Natural, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH: Mice, Transgenic, T cell, MESH: Natural Cytotoxicity Triggering Receptor 1, MESH: Muromegalovirus, Down-Regulation, Mice, Transgenic, Biology, Natural killer cell, Cell Line, 03 medical and health sciences, Immune system, MESH: Mice, Inbred C57BL, MESH: Antibodies, Blocking, medicine, Gene silencing, Animals, Antibodies, Blocking, Transcription factor, MESH: Mice, 030304 developmental biology, Natural Cytotoxicity Triggering Receptor 1, MESH: Transcription, Genetic, Genetic Complementation Test, MESH: Cell Line, Mice, Inbred C57BL, MESH: T-Lymphocytes, Amino Acid Substitution, MESH: Listeriosis, Mutagenesis, Immunology, Immunologic Memory, MESH: Adaptive Immunity, MESH: DNA-Binding Proteins, 030215 immunology, Transcription Factors
Abstract: Natural Killer Controls Cytolytic natural killer (NK) cells participate in both antimicrobial and antitumor immunity. Their responsiveness is tuned through signals received through a variety of inhibitory and activating receptors expressed on their cell surface. Narni-Mancinelli et al. (p. 344 ) now show that signaling through the activating receptor NKp46 paradoxically keeps NK cell responses in check. NK cells from mice with disrupted NKp46 expression were hyperresponsive to stimulation and better protected against viral infection. NK cell responses, which are part of the early response to infection, may thus need to be carefully tuned to ensure optimal initiation of adaptive immunity and formation of protective long-lived memory cells.
Published: 2012
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33. Listeria monocytogenes encephalitis in France

Author: Benoit Guery, Jean-Louis Herrmann, Alexandra Mailles, Cédric Bretonnière, Celine Bressollette-Bodin, Jean Paul Stahl, Daniel Terral, Marc Lecuit, Frederic Laurent, Cecile Bebear, Bruno POZZETTO, Alexandre Leclercq, Virginie Prendki, Institut de Veille Sanitaire (INVS), Laboratoire des Listeria, Centre National de Référence des Listeria, et Centre collaborateur de l’OMS pour la listériose d’origine alimentaire (CNR - CCOMS), Institut Pasteur [Paris], Service de maladies infectieuses et tropicales, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Institut de recherche en santé, environnement et travail (Irset), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Santé et de la Recherche Médicale (INSERM)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université d'Angers (UA), National Study on Listeriosis Encephalitis Steering Committee, Institut Pasteur [Paris] (IP), and Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Subjects: Male, Rhombencephalitis, MESH: Comorbidity, Comorbidity, medicine.disease_cause, MESH: Listeria monocytogenes, MESH: Genotype, 0302 clinical medicine, MESH: Aged, 80 and over, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Case fatality rate, MESH: Immunocompromised Host, Listeriosis, Prospective Studies, Prospective cohort study, Aged, 80 and over, MESH: Aged, 0303 health sciences, MESH: Middle Aged, Serogénotype, Cerebrospinal Fluid Proteins, Middle Aged, 3. Good health, Infectious Diseases, Population Surveillance, National study, Encephalitis, Female, France, medicine.medical_specialty, Genotype, Corticosteroid treatment, MESH: Population Surveillance, 03 medical and health sciences, Immunocompromised Host, Listeria monocytogenes, Internal medicine, medicine, Humans, Serotyping, Aged, MESH: Humans, 030306 microbiology, business.industry, Cancer, MESH: Serotyping, MESH: Cerebrospinal Fluid Proteins, medicine.disease, MESH: Male, MESH: Prospective Studies, MESH: France, MESH: Listeriosis, Immunology, Etiology, MESH: Encephalitis, business, MESH: Female, 030217 neurology & neurosurgery
Abstract: Purpose The authors had for aim to describe the characteristics of listeriosis encephalitis patients enrolled in a national study. Methods Listeria monocytogenes was identified as the forth etiology of encephalitis, in a prospective study in France, in 2007. We analyzed 12 cases with confirmed listeriosis (seven with rhombencephalitis, five with encephalitis) and compared them with encephalitis of other etiologies and with listeriosis encephalitis, mandatorily notified during the same year. Results L . monocytogenes strains were genoserotyped as IVb ( n = 6), IIa ( n = 3), and IIb ( n = 3). Cerebrospinal fluid (CSF) median protein level was 2.5 g/L and the median WBC counts was 367 cells/mm 3 . Among twelve patients, seven patients had ongoing comorbidities. The case fatality rate during hospitalization was 50%. Patients presenting with listeriosis enrolled in the study did not differ from those reported to the mandatory surveillance system according to their demographical characteristics and comorbidities. They were older, presented more frequently with cancer, or were more often under corticosteroid treatment than patients presenting with encephalitis due to other etiologies. Conclusions Neurolisteriosis has a poor prognosis, thus it should be considered early in patients presenting with encephalitis to improve its management.
Published: 2011
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34. TLR2 and RIP2 Pathways Mediate Autophagy of Listeria monocytogenes via Extracellular Signal-regulated Kinase (ERK) Activation*

Author: Amal O. Amer, Thirumala-Devi Kanneganti, Mohamed Lamkanfi, Douglas R. Green, Jacques Pouysségur, Stephen W.G. Tait, Maureen A. McGargill, Paras K. Anand, Gabriel Núñez, Gilles Pagès, School of Biochemistry and Immunology, Institute of Developmental Biology and Cancer (IBDC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Department of Immunology, and St Jude Children's Research Hospital
Subjects: MESH: Signal Transduction, MAPK/ERK pathway, MESH: Mice, Mutant Strains, Nod2 Signaling Adaptor Protein, MESH: Listeria monocytogenes, medicine.disease_cause, Biochemistry, Mice, 0302 clinical medicine, INFECTION, MESH: Nod2 Signaling Adaptor Protein, ADAPTIVE IMMUNITY, MESH: Animals, Listeriosis, Receptor, Extracellular Signal-Regulated MAP Kinases, MESH: Extracellular Signal-Regulated MAP Kinases, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Cells, Cultured, 0303 health sciences, EARLY PHASE, CRYOPYRIN INFLAMMASOME, MESH: Toll-Like Receptor 2, EPITHELIAL-CELLS, CROHN-DISEASE, Acquired immune system, 3. Good health, Cell biology, 030220 oncology & carcinogenesis, Receptor-Interacting Protein Serine-Threonine Kinases, MESH: Microscopy, Electron, Transmission, Signal transduction, MESH: Cells, Cultured, Signal Transduction, Immunology, Blotting, Western, MESH: Receptor-Interacting Protein Serine-Threonine Kinases, Biology, INTRACELLULAR PATHOGENS, Cell Line, 03 medical and health sciences, HOST-DEFENSE, Listeria monocytogenes, Microscopy, Electron, Transmission, Receptor-Interacting Protein Serine-Threonine Kinase 2, medicine, Autophagy, MESH: Autophagy, MESH: Blotting, Western, Animals, GENOME-WIDE ASSOCIATION, MESH: Mice, Molecular Biology, 030304 developmental biology, Innate immune system, Macrophages, MESH: Macrophages, Biology and Life Sciences, Cell Biology, digestive system diseases, Mice, Mutant Strains, Toll-Like Receptor 2, MESH: Cell Line, TLR2, MESH: Listeriosis, INNATE IMMUNITY
Abstract: International audience; Listeria monocytogenes is a facultative intracellular pathogen that invades both phagocytic and non-phagocytic cells. Recent studies have shown that L. monocytogenes infection activates the autophagy pathway. However, the innate immune receptors involved and the downstream signaling pathways remain unknown. Here, we show that macrophages deficient in the TLR2 and NOD/RIP2 pathway display defective autophagy induction in response to L. monocytogenes. Inefficient autophagy in Tlr2(-/-) and Nod2(-/-) macrophages led to a defect in bacteria colocalization with the autophagosomal marker GFP-LC3. Consequently, macrophages lacking TLR2 and NOD2 were found to be more susceptible to L. monocytogenes infection, as were the Rip2(-/-) mice. Tlr2(-/-) and Nod2(-/-) cells showed perturbed NF-κB and ERK signaling. However, autophagy against L. monocytogenes was dependent selectively on the ERK pathway. In agreement, wild-type cells treated with a pharmacological inhibitor of ERK or ERK-deficient cells displayed inefficient autophagy activation in response to L. monocytogenes. Accordingly, fewer bacteria were targeted to the autophagosomes and, consequently, higher bacterial growth was observed in cells deficient in the ERK signaling pathway. These findings thus demonstrate that TLR2 and NOD proteins, acting via the downstream ERK pathway, are crucial to autophagy activation and provide a mechanistic link between innate immune receptors and induction of autophagy against cytoplasm-invading microbes, such as L. monocytogenes.
Published: 2011
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35. Transcytosis of Listeria monocytogenes across the intestinal barrier upon specific targeting of goblet cell accessible E-cadherin

Author: Pascale Cossart, Théodora Niault, Marc Lecuit, Olivier Disson, Georgios Nikitas, Chantal Deschamps, Microorganismes et Barrières de l'Hôte (Equipe avenir), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'infectiologie Necker-Pasteur [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut Pasteur [Paris], Centre National de Référence des Listeria-G5 Microorganismes et Barrières de l'Hôte (CNR-CCOMS), Institut Pasteur [Paris], Georgios Nikitas received a Marie Curie Early Stage Research Training fellowship from the Intrapath program, EU FP6. We thank Institut Pasteur, Institut National de la Santé et de la Recherche Médicale, Fondation pour la Recherche Médicale, Ville de Paris, Fondation BNP Paribas, and the European Research Council for financial support., We thank the members of the Microbes and Host Barriers Group for their support and Matteo Bonazzi and the personnel of the Imagopole Facility for their help and support. We thank Gerard Eberl for helpful discussion and advice., European Project: 35418,INTRAPATH, Equipe avenir Microorganismes et Barrières de l'Hôte, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and Institut Pasteur [Paris] (IP)
Subjects: MESH: Transcytosis, INVASION, MESH: Listeria monocytogenes, CAMPYLOBACTER-JEJUNI, MESH: Cadherins, Mice, Intestinal mucosa, Immunology and Allergy, Listeriosis, MESH: Animals, Intestinal Mucosa, IN-VIVO, 0303 health sciences, MESH: Exocytosis, Tight junction, SURFACE PROTEIN, Cell Polarity, respiratory system, Cadherins, Intestinal epithelium, 3. Good health, Cell biology, TRANSLOCATION, medicine.anatomical_structure, Transcytosis, MESH: Intestinal Mucosa, Goblet Cells, NEISSERIA-GONORRHOEAE, MESH: Cell Polarity, EPITHELIAL TIGHT JUNCTION, MESH: Mice, Transgenic, Immunology, Mice, Transgenic, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, digestive system, Exocytosis, Article, Microbiology, Adherens junction, 03 medical and health sciences, MESH: Mice, Inbred C57BL, medicine, Animals, Humans, ESCHERICHIA-COLI K1, MESH: Mice, 030304 developmental biology, Goblet cell, Mucous Membrane, MESH: Humans, 030306 microbiology, Cadherin, MESH: Mucous Membrane, Listeria monocytogenes, Epithelium, Mice, Inbred C57BL, MESH: Listeriosis, MESH: Biomarkers, CACO-2 CELLS, MESH: Goblet Cells, Biomarkers, RAT SMALL-INTESTINE
Abstract: Listeria monocytogenes targets accessible E-cadherin expressed on mucus-producing goblet cells to invade the intestinal tissue., Listeria monocytogenes (Lm) is a foodborne pathogen that crosses the intestinal barrier upon interaction between its surface protein InlA and its species-specific host receptor E-cadherin (Ecad). Ecad, the key constituent of adherens junctions, is typically situated below tight junctions and therefore considered inaccessible from the intestinal lumen. In this study, we investigated how Lm specifically targets its receptor on intestinal villi and crosses the intestinal epithelium to disseminate systemically. We demonstrate that Ecad is luminally accessible around mucus-expelling goblet cells (GCs), around extruding enterocytes at the tip and lateral sides of villi, and in villus epithelial folds. We show that upon preferential adherence to accessible Ecad on GCs, Lm is internalized, rapidly transcytosed across the intestinal epithelium, and released in the lamina propria by exocytosis from where it disseminates systemically. Together, these results show that Lm exploits intrinsic tissue heterogeneity to access its receptor and reveal transcytosis as a novel and unanticipated pathway that is hijacked by Lm to breach the intestinal epithelium and cause systemic infection.
Published: 2011
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36. Single-cell techniques using chromosomally tagged fluorescent bacteria to study Listeria monocytogenes infection processes

Author: Olivier Dussurget, Alejandro Toledo-Arana, Marie-Anne Nahori, Diego Alignani, Javier Pizarro-Cerdá, Melanie Hamon, Pascale Cossart, Laurent Dortet, Damien Balestrino, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Régulation Immunitaire et Vaccinologie, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by Institut Pasteur, INSERM, INRA, and ERC (advanced grant 233348). A.T.-A. was an EMBO long-term fellow. D.B. was supported by INRA. L.D. was supported by Fondation pour la Recherche Médicale (FRM). P.C. is an international research scholar of the Howard Hughes Medical Institute., European Project: 233348,EC:FP7:ERC,ERC-2008-AdG,MODELIST(2009), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Vacuole, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, MESH: Bacteriological Techniques, medicine.disease_cause, MESH: Listeria monocytogenes, Applied Microbiology and Biotechnology, Microbiology, Flow cytometry, MESH: Recombinant Proteins, Mice, 03 medical and health sciences, Plasmid, Listeria monocytogenes, In vivo, MESH: Plasmids, Methods, medicine, Animals, Listeriosis, MESH: Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, MESH: Mice, 030304 developmental biology, Bacteriological Techniques, 0303 health sciences, Staining and Labeling, Ecology, medicine.diagnostic_test, 030306 microbiology, Intracellular parasite, MESH: Molecular Biology, Cell cycle, Recombinant Proteins, 3. Good health, Luminescent Proteins, MESH: Staining and Labeling, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Listeriosis, MESH: Luminescent Proteins, Intracellular, Plasmids, Food Science, Biotechnology
Abstract: 12 p., 2 tables, 5 figures and bibliography, Listeria monocytogenes is a Gram-positive facultative intracellular pathogen which invades different cell types, including nonphagocytic cells, where it is able to replicate and survive. The different steps of the cellular infectious process have been well described and consist of bacterial entry, lysis of the endocytic vacuole, intracellular replication, and spreading to neighboring cells. To study the listerial infectious process, gentamicin survival assays, plaque formation, and direct microscopy observations are typically used; however, there are some caveats with each of these techniques. In this study we describe new single-cell techniques based on use of an array of integrative fluorescent plasmids (green, cyan, and yellow fluorescent proteins) to easily, rapidly, and quantitatively detect L. monocytogenes in vitro and in vivo. We describe construction of 13 integrative and multicopy plasmids which can be used for detecting intracellular bacteria, for measuring invasion, cell-to-cell spreading, and intracellular replication, for monitoring in vivo infections, and for generating transcriptional or translational reporters. Furthermore, we tested these plasmids in a variety of epifluorescence- and flow cytometry-based assays. We showed that we could (i) determine the expression of a particular promoter during the cell cycle, (ii) establish in one rapid experiment at which step in the cell cycle a particular mutant is defective, and (iii) easily measure the number of infected cells in vitro and in mouse organs. The plasmids that are described and the methods to detect them are new powerful tools to study host-Listeria interactions in a fast, robust, and high-throughput manner., This work was supported by Institut Pasteur, INSERM, INRA, and ERC (advanced grant 233348). A.T.-A. was an EMBO long-term fellow. D.B. was supported by INRA. L.D. was supported by Fondation pour la Recherche Médicale (FRM).
Published: 2010
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37. Listeria monocytogenes impairs SUMOylation for efficient infection

Author: Melanie Hamon, Kris Gevaert, Edith Gouin, Joël Vandekerckhove, Anne Dejean, Hélène Neyret-Kahn, Marie-Anne Nahori, Pascale Cossart, Francis Impens, David Ribet, USC2020, Inst Natl Rech Agron, Institut Pasteur [Paris], Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Pharmacologie Cellulaire, Institut Pasteur [Paris] (IP), Organisation Nucléaire et Oncogenèse, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Universiteit Gent = Ghent University (UGENT)
Subjects: listeria, [SDV]Life Sciences [q-bio], SUMO protein, MESH: Heat-Shock Proteins, MESH: Listeria monocytogenes, medicine.disease_cause, ACTIVATION, Hemolysin Proteins, Mice, Listeriosis, MESH: Animals, Heat-Shock Proteins, POSTTRANSLATIONAL MODIFICATIONS, 0303 health sciences, Multidisciplinary, biology, 030302 biochemistry & molecular biology, Listeriolysin O, 3. Good health, Cell biology, [SDV] Life Sciences [q-bio], Histone, MESH: Hemolysin Proteins, Small Ubiquitin-Related Modifier Proteins, LLO, PROTEASE, Virulence Factors, PROTEINS, Bacterial Toxins, EFFECTOR, ubiquitin-like protein, Article, Cell Line, Microbiology, 03 medical and health sciences, Listeria monocytogenes, Heat shock protein, medicine, Animals, Humans, MESH: Mice, MESH: Virulence Factors, 030304 developmental biology, MESH: Humans, YOPJ, Pneumolysin, Pathogenic bacteria, biology.organism_classification, MESH: Ubiquitin-Conjugating Enzymes, MESH: Cell Line, MESH: Bacterial Toxins, MESH: Small Ubiquitin-Related Modifier Proteins, post-translational modification, MESH: Listeriosis, SUMO, MESH: Protein Processing, Post-Translational, Ubiquitin-Conjugating Enzymes, MESH: HeLa Cells, CELLS, biology.protein, Listeria, Protein Processing, Post-Translational, HeLa Cells
Abstract: During infection, pathogenic bacteria manipulate the host cell in various ways to allow their own replication, propagation and escape from host immune responses. Post-translational modifications are unique mechanisms that allow cells to rapidly, locally and specifically modify activity or interactions of key proteins. Some of these modifications, including phosphorylation and ubiquitylation, can be induced by pathogens. However, the effects of pathogenic bacteria on SUMOylation, an essential post-translational modification in eukaryotic cells, remain largely unknown. Here we show that infection with Listeria monocytogenes leads to a decrease in the levels of cellular SUMO-conjugated proteins. This event is triggered by the bacterial virulence factor listeriolysin O (LLO), which induces a proteasome-independent degradation of Ubc9, an essential enzyme of the SUMOylation machinery, and a proteasome-dependent degradation of some SUMOylated proteins. The effect of LLO on Ubc9 is dependent on the pore-forming capacity of the toxin and is shared by other bacterial pore-forming toxins like perfringolysin O (PFO) and pneumolysin (PLY). Ubc9 degradation was also observed in vivo in infected mice. Furthermore, we show that SUMO overexpression impairs bacterial infection. Together, our results reveal that Listeria, and probably other pathogens, dampen the host response by decreasing the SUMOylation level of proteins critical for infection.
Published: 2010
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38. Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in France

Author: Sylvie Brémont, A. Morvan, Alexandre Leclercq, Carole Ayoub Moubareck, Marc Lecuit, A. Le Monnier, M. Hervé-Bazin, Patrice Courvalin, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre national de Référence Résistance aux Antibiotiques (CNR), Institut Pasteur [Paris], Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Microorganismes et Barrières de l'Hôte (Equipe avenir), Centre Hospitalier de Versailles André Mignot (CHV), This study was supported by the Institut Pasteur (Paris, France) and the Institut de Veille Sanitaire (Saint Maurice, France)., We thank Yves Pechine for statistical analysis., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut Pasteur [Paris] (IP)
Subjects: Time Factors, [SDV]Life Sciences [q-bio], Antibiotics, Drug resistance, MESH: Base Sequence, medicine.disease_cause, MESH: Listeria monocytogenes, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Pharmacology (medical), Listeriosis, MESH: Evolution, Molecular, 0303 health sciences, MESH: Microbial Sensitivity Tests, Molecular Epidemiology, 3. Good health, Anti-Bacterial Agents, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: History, 20th Century, France, MESH: History, 21st Century, medicine.drug, MESH: DNA Primers, Gene Transfer, Horizontal, medicine.drug_class, Tetracycline, Microbial Sensitivity Tests, Biology, In Vitro Techniques, History, 21st Century, Microbiology, Evolution, Molecular, 03 medical and health sciences, Antibiotic resistance, Listeria monocytogenes, Mechanisms of Resistance, MESH: Anti-Bacterial Agents, MESH: Drug Resistance, Bacterial, Drug Resistance, Bacterial, medicine, MESH: Molecular Epidemiology, Humans, 030304 developmental biology, DNA Primers, Pharmacology, MESH: In Vitro Techniques, MESH: Humans, Molecular epidemiology, Base Sequence, 030306 microbiology, MESH: Time Factors, History, 20th Century, Virology, Trimethoprim, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Gene Transfer, Horizontal, Penicillin, MESH: France, MESH: Listeriosis, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Susceptibility to antibiotics of 4,816 clinical L. monocytogenes strains isolated since 1926 was studied, and the temporal evolution of susceptibility to antibiotics was analyzed through several decades. The mechanisms of resistance in each resistant strain were studied. The prevalence of resistant strains was estimated at 1.27% among isolates from humans. Resistance to tetracyclines+ and fluoroquinolones was more common and has recently emerged. Although acquired resistance in clinical L. monocytogenes did not implicate clinically relevant antibiotics, the possibility of resistance gene transfers, the description of the first clinical isolate with high-level resistance to trimethoprim, and the recent increase in penicillin MICs up to 2 μg/ml reinforce the need for microbiological surveillance.
Published: 2010
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39. Visualizing early splenic memory CD8+ T cells reactivation against intracellular bacteria in the mouse

Author: Grégoire Lauvau, Marc Bajénoff, Frédéric Brau, Emilie Narni-Mancinelli, Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Immunologie des maladies infectieuses allergiques et autoimmunes, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM : AVENIR, ANR-08-MIEN-0008,EMICIF,Caractérisation in vivo des lymphocytes T CD8 effecteurs et mémoires antimicrobiens au cours de réponses immunitaires primaires et secondaires: mécanismes d'induction, fonctions protectrices, et interactions avec les cellules de l'immunité innée(2008), ANR-08-JCJC-0134,RCS,Rôle des cellules stromales dans l'organisation et la fonction des organes lymphoides secondaires(2008), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Université Nice Sophia Antipolis (... - 2019) (UNS), Centre d'Immunologie de Marseille - Luminy ( CIML ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Aix Marseille Université ( AMU ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de pharmacologie moléculaire et cellulaire ( IPMC ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), ANR-08-MIEN-0008,EMICIF,Caractérisation in vivo des lymphocytes T CD8 effecteurs et mémoires antimicrobiens au cours de réponses immunitaires primaires et secondaires: mécanismes d'induction, fonctions protectrices, et interactions avec les cellules de l'immunité innée ( 2008 ), and ANR-08-JCJC-0134,RCS,Rôle des cellules stromales dans l'organisation et la fonction des organes lymphoides secondaires ( 2008 )
Subjects: MESH: Spleen, lcsh:Medicine, MESH: Flow Cytometry, CD8-Positive T-Lymphocytes, MESH: Listeria monocytogenes, MESH: Receptors, CCR7, Interleukin 21, Mice, 0302 clinical medicine, MESH : Chemokine CCL3, MESH : Receptors, CCR7, Cytotoxic T cell, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH: L-Selectin, Listeriosis, MESH: Animals, IL-2 receptor, L-Selectin, lcsh:Science, Chemokine CCL3, 0303 health sciences, Mice, Inbred BALB C, Multidisciplinary, ZAP70, MESH : CD8-Positive T-Lymphocytes, Acquired immune system, Flow Cytometry, MESH: CD8-Positive T-Lymphocytes, Cell biology, Infectious Diseases, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH : L-Selectin, Research Article, MESH : Spleen, Receptors, CCR7, MESH : Flow Cytometry, MESH: Interferon-gamma, Immunology, MESH: Mice, Inbred BALB C, MESH : Mice, Inbred C57BL, Biology, 03 medical and health sciences, Interferon-gamma, Immune system, MESH: Mice, Inbred C57BL, Immunology/Immunity to Infections, MESH : Mice, Animals, MESH: Chemokine CCL3, MESH: Mice, MESH : Mice, Inbred BALB C, MESH : Interferon-gamma, 030304 developmental biology, Interleukin 3, lcsh:R, Listeria monocytogenes, Mice, Inbred C57BL, MESH: Listeriosis, lcsh:Q, MESH : Animals, MESH : Listeria monocytogenes, MESH : Listeriosis, CD8, Spleen, 030215 immunology
Abstract: International audience; Memory CD8(+) T cells represent an important effector arm of the immune response in maintaining long-lived protective immunity against viruses and some intracellular bacteria such as Listeria monocytogenes (L.m). Memory CD8(+) T cells are endowed with enhanced antimicrobial effector functions that perfectly tail them to rapidly eradicate invading pathogens. It is largely accepted that these functions are sufficient to explain how memory CD8(+) T cells can mediate rapid protection. However, it is important to point out that such improved functional features would be useless if memory cells were unable to rapidly find the pathogen loaded/infected cells within the infected organ. Growing evidences suggest that the anatomy of secondary lymphoid organs (SLOs) fosters the cellular interactions required to initiate naive adaptive immune responses. However, very little is known on how the SLOs structures regulate memory immune responses. Using Listeria monocytogenes (L.m) as a murine infection model and imaging techniques, we have investigated if and how the architecture of the spleen plays a role in the reactivation of memory CD8(+) T cells and the subsequent control of L.m growth. We observed that in the mouse, memory CD8(+) T cells start to control L.m burden 6 hours after the challenge infection. At this very early time point, L.m-specific and non-specific memory CD8(+) T cells localize in the splenic red pulp and form clusters around L.m infected cells while naïve CD8(+) T cells remain in the white pulp. Within these clusters that only last few hours, memory CD8(+) T produce inflammatory cytokines such as IFN-gamma and CCL3 nearby infected myeloid cells known to be crucial for L.m killing. Altogether, we describe how memory CD8(+) T cells trafficking properties and the splenic micro-anatomy conjugate to create a spatio-temporal window during which memory CD8(+) T cells provide a local response by secreting effector molecules around infected cells.
Published: 2010
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40. Human Listeriosis Caused by Listeria ivanovii

Author: Olivier Disson, Alban Le Monnier, Magdalena K. Bielecka, Olivier Join-Lambert, Christelle Guillet, Marie-France Mamzer-Bruneel, Patrick Berche, Mariela Scortti, Marc Lecuit, Alexandre Leclercq, Frédéric Méchaï, Olivier Lortholary, José A. Vázquez-Boland, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris], University of Edinburgh, Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Institut National de la Santé et de la Recherche Médicale (INSERM), Universidad de León [León], M.L. received financial support from Assistance Publique–Hôpitaux de Paris, Université Paris Descartes, Institut Pasteur, Institut de Veille Sanitaire, and Institut National de la Santé et de la Recherche Médicale. J.V.-B. received Wellcome Trust program grant 074020., We thank Véronique Goulet for helpful discussions., and Institut Pasteur [Paris] (IP)
Subjects: Male, Epidemiology, [SDV]Life Sciences [q-bio], lcsh:Medicine, Human pathogen, MESH: Listeria, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Immunocompromised Host, Listeriosis, MESH: Animals, bacteria, MESH: Bacteremia, 0303 health sciences, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, MESH: Middle Aged, biology, Goats, MESH: Paris, dispatch, Middle Aged, 3. Good health, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Listeria ivanovii, gastroenteritis, Microbiology (medical), Paris, Listeria, kidney transplantation, Opportunistic Infections, MESH: Goats, Microbiology, lcsh:Infectious and parasitic diseases, Immunocompromised Host, 03 medical and health sciences, medicine, Animals, Humans, lcsh:RC109-216, bacteremia, 030304 developmental biology, MESH: Humans, 030306 microbiology, lcsh:R, MESH: Opportunistic Infections, biology.organism_classification, medicine.disease, Virology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Gastroenteritis, MESH: Male, MESH: Listeriosis, Bacteremia, Bacteria, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Two species of Listeria are pathogenic; L. monocytogenes infects humans and animals, and L. ivanovii has been considered to infect ruminants only. We report L. ivanovii–associated gastroenteritis and bacteremia in a man. This isolate was indistinguishable from prototypic ruminant strains. L. ivanovii is thus an enteric opportunistic human pathogen.The genus Listeria contains 2 pathogenic species, L. monocytogenes and L. ivanovii (1). They both invade host cells, replicate in the cytosol after phagosomal escape, and spread from cell to cell by polymerizing actin. These mechanisms correlate with the presence in each species of genetic determinants called the inlAB internalization locus, the LIPI-1 intracellular survival pathogenicity island, and the hpt intracellular growth locus (2). However, each species appears to infect different hosts: L. monocytogenes infects humans and ruminants, whereas L. ivanovii is thought to infect ruminants only (2). L. ivanovii have been previously isolated, although rarely, from infected humans, indicating pathogenic potential for humans (Table). We report a case of L. ivanovii infection in a man with a kidney transplant. The ecology of L. ivanovii suggests that the rarity of human listeriosis due to this species reflects not only host tropism factors but also the rare occurrence of this species in the environment, compared with L. monocytogenes.
Published: 2010
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41. Investigation of specific substitutions in virulence genes characterizing phenotypic groups of low-virulence field strains of Listeria monocytogenes

Author: Christine Jacquet, Isabelle Albert, S. Témoin, A. Kerouanton, Eliane Milohanic, Sylvie M. Roche, P. Gracieux, Pascale Cossart, Philippe Velge, Olivier Grépinet, Isabelle Virlogeux-Payant, Unité de Pathologie Infectieuse et Immunologie [Nouzilly] (PII), Institut National de la Recherche Agronomique (INRA), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Génomique des Microorganismes Pathogènes, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Unité de biométrie et intelligence artificielle de Jouy (MIA-JOUY), Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire des Listeria, Centre National de Référence des Listeria, et Centre collaborateur de l’OMS pour la listériose d’origine alimentaire (CNR - CCOMS), Institut Pasteur [Paris] (IP), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Unité de biométrie et intelligence artificielle de jouy, and Institut Pasteur [Paris]
Subjects: MESH: Sequence Analysis, DNA, Phospholipase, MESH: Virulence, medicine.disease_cause, MESH: Listeria monocytogenes, Applied Microbiology and Biotechnology, Mice, Genotype, Listeriosis, MESH: Animals, MESH: Bacterial Proteins, MESH: Evolution, Molecular, Genetics, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, 0303 health sciences, Ecology, biology, LISTERIA MONOCYTOGENES, SEQUENCE ANALYSIS, MESH: Amino Acid Substitution, Phenotype, Female, MESH: Type C Phospholipases, MESH: Peptide Termination Factors, Peptide Termination Factors, Biotechnology, Phosphatidylinositol phospholipase C activity, Phosphatidylcholine phospholipase C activity, Virulence, MESH: Phenotype, Cell Line, Microbiology, Evolution, Molecular, 03 medical and health sciences, Bacterial Proteins, Listeria monocytogenes, medicine, Animals, Humans, MOLECULAR SEQUENCE DATA, Gene, TYPE C PHOSPHOLIPASE, MESH: Mice, 030304 developmental biology, MESH: Molecular Sequence Data, MESH: Humans, 030306 microbiology, Sequence Analysis, DNA, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, EVOLUTION, MESH: Cell Line, Amino Acid Substitution, MESH: Listeriosis, Type C Phospholipases, Food Microbiology, VIRULENCE, MESH: Female, Bacteria, Food Science
Abstract: Several models have shown that virulence varies from one strain of Listeria monocytogenes to another, but little is known about the cause of low virulence. Twenty-six field L. monocytogenes strains were shown to be of low virulence in a plaque-forming assay and in a subcutaneous inoculation test in mice. Using the results of cell infection assays and phospholipase activities, the low-virulence strains were assigned to one of four groups by cluster analysis and then virulence-related genes were sequenced. Group I included 11 strains that did not enter cells and had no phospholipase activity. These strains exhibited a mutated PrfA; eight strains had a single amino acid substitution, PrfAK220T, and the other three had a truncated PrfA, PrfAΔ174-237. These genetic modifications could explain the low virulence of group I strains, since mutated PrfA proteins were inactive. Group II and III strains entered cells but did not form plaques. Group II strains had low phosphatidylcholine phospholipase C activity, whereas group III strains had low phosphatidylinositol phospholipase C activity. Several substitutions were observed for five out of six group III strains in the plcA gene and for one out of three group II strains in the plcB gene. Group IV strains poorly colonized spleens of mice and were practically indistinguishable from fully virulent strains on the basis of the above-mentioned in vitro criteria. These results demonstrate a relationship between the phenotypic classification and the genotypic modifications for at least group I and III strains and suggest a common evolution of these strains within a group.
Published: 2005
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42. Auto, a surface associated autolysin of Listeria monocytogenes required for entry into eukaryotic cells and virulence

Author: Didier, Cabanes, Olivier, Dussurget, Pierre, Dehoux, Pascale, Cossart, Interactions Bactéries-Cellules, Institut Pasteur [Paris] (IP), Intégration et Analyse Génomique (Plate-Forme 4) (PF4), Work in the P.C. laboratory received financial support from the Pasteur Institute, the Ministère de l’Education Nationale, de la Recherche et de la Technologie (Programme de Recherche Fondamentale en Microbiologie et Maladies Infectieuses et Parasitaires), by the ACI Microbiology 2003 and by the European Commission (contract QLG2-CT 1999–00932). P.C. is an international scholar from the Howard Hughes Medical Institute., Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut Pasteur [Paris]
Subjects: MESH: Spleen, Guinea Pigs, MESH: Mice, Inbred BALB C, MESH: Lymph Nodes, MESH: Amino Acid Sequence, MESH: Virulence, MESH: Listeria monocytogenes, MESH: Eukaryotic Cells, Bacterial Adhesion, Cell Line, MESH: Guinea Pigs, Mice, MESH: Brain, Bacterial Proteins, Animals, Humans, Listeriosis, AUTOLYSIN, MESH: Animals, Amino Acid Sequence, MESH: Bacterial Adhesion, MESH: Mice, MESH: Bacterial Proteins, Mice, Inbred BALB C, MESH: Humans, SURFACE PROTEIN, LISTERIA MONOCYTOGENES, Brain, Membrane Proteins, N-Acetylmuramoyl-L-alanine Amidase, CELL WALL ANCHORING, MESH: Cell Line, Intestines, Eukaryotic Cells, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Liver, MESH: Listeriosis, MICROBIAL INFECTION STRATEGY, MESH: N-Acetylmuramoyl-L-alanine Amidase, VIRULENCE, Female, Lymph Nodes, MESH: Membrane Proteins, MESH: Female, Spleen, MESH: Intestines, MESH: Liver
Abstract: International audience; Listeria monocytogenes is an opportunistic food-borne human and animal pathogen. Several surface proteins expressed by this intracellular pathogen are critical for the infectious process. By in silico analysis we compared the surface protein repertories of L. monocytogenes and of the non-pathogenic species Listeria innocua and identified a gene encoding a surface protein of L. monocytogenes absent in L. innocua. This gene that we named aut encodes a protein (Auto) of 572 amino acids containing a signal sequence, a N-terminal autolysin domain and a C-terminal cell wall-anchoring domain made up of four GW modules. We show here that the aut gene is expressed independently of the virulence gene regulator PrfA and encodes a surface protein with an autolytic activity. We provide evidence that Auto is required for entry of L. monocytogenes into cultured non-phagocytic eukaryotic cells. The low invasiveness of an aut deletion mutant correlates with its reduced virulence following intravenous inoculation of mice and oral infection of guinea pigs. During infection, the autolytic activity of Auto may also be critical. Auto appears thus as a novel type of L. monocytogenes virulence factor.
Published: 2004
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43. Listeria monocytogenes bile salt hydrolase is a PrfA-regulated virulence factor involved in the intestinal and hepatic phases of listeriosis

Author: Dussurget, O., Cabanes, D., Dehoux, P., Marc Lecuit, Buchrieser, C., Glaser, P., Cossart, P., European Listeria Genome Consortium, Interactions Bactéries-Cellules, Institut Pasteur [Paris], Génomique des Microorganismes Pathogènes, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), The European Listeria Genome Consortium (P. Glaser, L. Frangeul, C. Buchrieser, C. Rusniok, A. Amend, F. Baquero, P. Berche, H. Bloecker, P. Brandt, T. Chakraborty, A. Charbit, F. Chetouani, E. Couve, A. de Daruvar, P. Dehoux, E. Domann, G. Dominguez-Bernal, E. Duchaud, L. Durant, O. Dussurget, K.-D. Entian, H. Fsihi, F. Garcia-Del Portillo, P. Garrido, L. Gautier, W. Goebel, N. Gomez-Lopez, T. Hain, J. Hauf, D. Jackson, L.-M. Jones, U. Kaerst, J. Kreft, M. Kuhn, F. Kunst, G. Kurapkat, E. Madueno, A. Maitournam, J. Mata Vicente, E. Ng, H. Nedjari, G. Nordsiek, S. Novella, B. de Pablos, J.-C. Perez-Diaz, R. Purcell, B. Remmel, M. Rose, T. Schlueter, N. Simoes, A. Tierrez, J.-A. Vazquez-Boland, H. Voss, J. Wehland and P. Cossart), Institut Pasteur [Paris] (IP), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Subjects: MESH: Amidohydrolases, [SDV]Life Sciences [q-bio], MESH: Virulence, MESH: Base Sequence, medicine.disease_cause, MESH: Listeria monocytogenes, Virulence factor, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Putative gene, Listeriosis, Choloylglycine hydrolase, Pathogen, MESH: Bacterial Proteins, MESH: Mutagenesis, 0303 health sciences, MESH: Gene Expression Regulation, Bacterial, Virulence, MESH: Gene Expression Regulation, Enzymologic, Liver Diseases, 3. Good health, Gastroenteritis, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Genes, Bacterial, MESH: Oxygen, MESH: Peptide Termination Factors, Peptide Termination Factors, DNA, Bacterial, MESH: Liver Diseases, MESH: Trans-Activators, Molecular Sequence Data, Biology, Microbiology, MESH: Sequence Homology, Nucleic Acid, Gene Expression Regulation, Enzymologic, Amidohydrolases, 03 medical and health sciences, Listeria monocytogenes, Bacterial Proteins, Sequence Homology, Nucleic Acid, medicine, Molecular Biology, Gene, 030304 developmental biology, MESH: Molecular Sequence Data, Base Sequence, 030306 microbiology, Gene Expression Regulation, Bacterial, biology.organism_classification, MESH: DNA, Bacterial, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Gastroenteritis, Oxygen, MESH: Listeriosis, Genes, Bacterial, Mutagenesis, Listeria, Trans-Activators, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Listeria monocytogenes is a bacterial pathogen causing severe food-borne infections in humans and animals. It can sense and adapt to a variety of harsh microenvironments outside as well as inside the host. Once ingested by a mammalian host, the bacterial pathogen reaches the intestinal lumen, where it encounters bile salts which, in addition to their role in digestion, have antimicrobial activity. Comparison of the L. monocytogenes and Listeria innocua genomes has revealed the presence of an L. monocytogenes-specific putative gene encoding a bile salt hydrolase (BSH). Here, we show that the bsh gene encodes a functional intracellular enzyme in all pathogenic Listeria species. The bsh gene is positively regulated by PrfA, the transcriptional activator of known L. monocytogenes virulence genes. Moreover, BSH activity increases at low oxygen concentration. Deletion of bsh results in decreased resistance to bile in vitro, reduced bacterial faecal carriage after oral infection of the guinea-pigs, reduced virulence and liver colonization after intravenous inoculation of mice. Taken together, these results demonstrate that BSH is a novel PrfA-regulated L. monocytogenes virulence factor involved in the intestinal and hepatic phases of listeriosis.
Published: 2002
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44. A Transgenic Model for Listeriosis: Role of Internalin in Crossing the Intestinal Barrier

Author: Michel Huerre, Jean Lefort, Sandrine Vandormael-Pournin, Pierre Gounon, Charles Babinet, Marc Lecuit, Catherine Dupuy, Pascale Cossart, Interactions Bactéries-Cellules (UIBC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Institut Pasteur [Paris], Pharmacologie Cellulaire, Histopathologie, Microscopie électronique, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), and Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Male, MESH: Neoplasm Proteins, MESH: Intestine, Small, MESH: Spleen, [SDV]Life Sciences [q-bio], Colony Count, Microbial, MESH: Lymph Nodes, MESH: Virulence, medicine.disease_cause, MESH: Listeria monocytogenes, MESH: Cadherins, Transgenic Model, Mice, MESH: Enterocytes, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Fatty Acid-Binding Protein 7, Intestine, Small, Listeriosis, MESH: Animals, Transgenes, MESH: Nerve Tissue Proteins, Intestinal Mucosa, Receptor, Promoter Regions, Genetic, MESH: Bacterial Proteins, 0303 health sciences, Multidisciplinary, Virulence, Cadherins, 3. Good health, Neoplasm Proteins, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Liver, MESH: Intestinal Mucosa, Fatty Acid-Binding Protein 7, MESH: Bacterial Translocation, Genetically modified mouse, MESH: Mice, Transgenic, Transgene, Guinea Pigs, Mice, Transgenic, Nerve Tissue Proteins, MESH: Transgenes, MESH: Carrier Proteins, Biology, Fatty Acid-Binding Proteins, MESH: Fatty Acid-Binding Proteins, Microbiology, MESH: Guinea Pigs, Guinea pig, 03 medical and health sciences, Listeria monocytogenes, Bacterial Proteins, MESH: Promoter Regions, Genetic, medicine, Animals, Humans, Internalin, MESH: Tumor Suppressor Proteins, MESH: Mice, MESH: Colony Count, Microbial, 030304 developmental biology, MESH: Humans, 030306 microbiology, Tumor Suppressor Proteins, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, Disease Models, Animal, Enterocytes, MESH: Listeriosis, Bacterial Translocation, Immunology, Lymph Nodes, MESH: Disease Models, Animal, Carrier Proteins, Spleen, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, MESH: Liver
Abstract: Listeria monocytogenes is responsible for severe food-borne infections, but the mechanisms by which bacteria cross the intestinal barrier are unknown. Listeria monocytogenes expresses a surface protein, internalin, that interacts with a host receptor, E-cadherin, to promote entry into human epithelial cells. Murine E-cadherin, in contrast to guinea pig E-cadherin, does not interact with internalin, excluding the mouse as a model for addressing internalin function in vivo. In guinea pigs and transgenic mice expressing human E-cadherin, internalin was found to mediate invasion of enterocytes and crossing of the intestinal barrier. These results illustrate how relevant animal models for human infections can be generated.
Published: 2001
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45. A single amino acid in E-cadherin responsible for host specificity towards the human pathogen Listeria monocytogenes

Author: Barry M. Gumbiner, Pascale Cossart, Shaynoor Dramsi, Cara J. Gottardi, Mary Fedor-Chaiken, Marc Lecuit, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Memorial Sloane Kettering Cancer Center [New York], University of Cincinnati (UC), This work received financial support from ARC (No. 9976), the Ministère de l'Education Nationale, de la Recherche et de la Technologie (Programme Microbiologie No. 61885), the Ministère de la Défense (CR97069), EEC (BMH4 CT 96–0659) and the Pasteur Institute., We thank L.Shapiro for Figure 3D, for helpful comments and critical reading of the manuscript, R.Brackenbury and P.Stragier for critical reading of the manuscript, W.Gellin for helpful comments, M.Mareel for the gift of LoVo, HCT‐8 and NMe cells, M.Takeichi for the gift of the HECD1 hybridoma cell line and Elβ1 cells, P.J.Kilshaw for the gift of L KB cells and mEcad cDNA, D.Rimm for the gift of partial hEcad cDNA, K.Wary for the gift of A431 cells, B.Geiger for the gift of CHO mEcad‐transfected cells, A.M.Valles and J.P.Thiery for the gift of NBT2 cells, R.Hurme for purification of the HECD1 antibody, M.Bracke for the gift of MB2 antibody, and R.M.Mège for the gift of anti‐LCAM antibody. We thank G.Milon and M.Lebastard for help in mouse in vivo studies. We also thank J.Mengaud for his help and support in preliminary experiments, H.Kiefer for help with the fluorescent cell sorting system, L.Frangeul for help with the NJplot program, and R.Hellio for help with confocal microscopy (the confocal microscope was purchased with a donation from Marcel and Liliane Pollack)., Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Models, Molecular, [SDV]Life Sciences [q-bio], Human pathogen, Plasma protein binding, MESH: Amino Acid Sequence, medicine.disease_cause, MESH: Listeria monocytogenes, MESH: Cadherins, Mice, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Animals, Listeriosis, MESH: Phylogeny, Pathogen, MESH: Bacterial Proteins, Phylogeny, 0303 health sciences, Mice, Inbred BALB C, General Neuroscience, Cadherins, MESH: Amino Acid Substitution, Endocytosis, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Endocytosis, Female, MESH: Models, Molecular, Protein Binding, Research Article, MESH: Rats, Proline, Recombinant Fusion Proteins, Guinea Pigs, Molecular Sequence Data, MESH: Mice, Inbred BALB C, Mutagenesis (molecular biology technique), Biology, General Biochemistry, Genetics and Molecular Biology, Microbiology, MESH: Guinea Pigs, Cell Line, 03 medical and health sciences, Listeria monocytogenes, Bacterial Proteins, Species Specificity, medicine, MESH: Recombinant Fusion Proteins, MESH: Protein Binding, MESH: Species Specificity, Animals, Humans, Internalin, Amino Acid Sequence, Molecular Biology, MESH: Mice, 030304 developmental biology, MESH: Humans, MESH: Molecular Sequence Data, MESH: Proline, General Immunology and Microbiology, 030306 microbiology, Cadherin, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Cell Line, Rats, Disease Models, Animal, Amino Acid Substitution, MESH: Listeriosis, Listeria, MESH: Disease Models, Animal, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Human E-cadherin promotes entry of the bacterial pathogen Listeria monocytogenes into mammalian cells by interacting with internalin (InlA), a bacterial surface protein. Here we show that mouse E-cadherin, although very similar to human E-cadherin (85% identity), is not a receptor for internalin. By a series of domain-swapping and mutagenesis experiments, we identify Pro16 of E-cadherin as a residue critical for specificity: a Pro→Glu substitution in human E-cadherin totally abrogates interaction, whereas a Glu→Pro substitution in mouse E-cadherin results in a complete gain of function. A correlation between cell permissivity and the nature of residue 16 in E-cadherins from several species is established. The location of this key specificity residue in a region of E-cadherin not involved in cell-cell adhesion and the stringency of the interaction demonstrated here have important consequences not only for the understanding of internalin function but also for the choice of the animal model to be used to study human listeriosis: mouse, albeit previously widely used, and rat appear as inappropriate animal models to study all aspects of human listeriosis, as opposed to guinea-pig, which now stands as a small animal of choice for future in vivo studies.
Published: 1999
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46. Identification of four new members of the internalin multigene family of Listeria monocytogenes EGD

Author: Shaynoor Dramsi, Pierre L. Goossens, Pierre Dehoux, Maryse Lebrun, Pascale Cossart, Interactions Bactéries-Cellules, Institut Pasteur [Paris], Immunophysiologie cellulaire, This study received ﬁnancial support from the Ministere de la Recherche (ACC-SV6), the EEC (BMH4 CT 96-0659), and the Pasteur Institute., and Institut Pasteur [Paris] (IP)
Subjects: MESH: Sequence Analysis, DNA, Transcription, Genetic, MESH: Sequence Homology, Amino Acid, MESH: Spleen, [SDV]Life Sciences [q-bio], Colony Count, Microbial, MESH: Amino Acid Sequence, MESH: Virulence, MESH: Listeria monocytogenes, MESH: Nucleic Acid Hybridization, Mice, Plasmid, Cell Wall, MESH: Blotting, Southern, MESH: Animals, Listeriosis, Cloning, Molecular, Peptide sequence, MESH: Bacterial Proteins, Sequence Deletion, Genetics, Recombination, Genetic, 0303 health sciences, MESH: Gene Expression Regulation, Bacterial, Mice, Inbred BALB C, Virulence, Chromosome Mapping, Nucleic Acid Hybridization, MESH: Sequence Deletion, Blotting, Southern, Infectious Diseases, Liver, Chromosomal region, MESH: Recombination, Genetic, Female, MESH: Protein Sorting Signals, Plasmids, Research Article, Signal peptide, Sequence analysis, Immunology, Molecular Sequence Data, MESH: Sequence Alignment, MESH: Mice, Inbred BALB C, Sequence alignment, Biology, Protein Sorting Signals, Microbiology, Chromosomes, 03 medical and health sciences, MESH: Cell Wall, Bacterial Proteins, MESH: Plasmids, Animals, Humans, Internalin, MESH: Cloning, Molecular, Amino Acid Sequence, Gene, MESH: Colony Count, Microbial, MESH: Mice, 030304 developmental biology, Repetitive Sequences, Nucleic Acid, MESH: Repetitive Sequences, Nucleic Acid, MESH: Humans, MESH: Molecular Sequence Data, Sequence Homology, Amino Acid, 030306 microbiology, MESH: Transcription, Genetic, Gene Expression Regulation, Bacterial, Sequence Analysis, DNA, Molecular biology, Listeria monocytogenes, MESH: Listeriosis, Parasitology, MESH: Chromosomes, MESH: Chromosome Mapping, MESH: Female, Sequence Alignment, Spleen, MESH: Liver
Abstract: International audience; Listeria monocytogenes is a bacterial pathogen that is able to invade nonphagocytic cells. Two surface proteins, internalin, the inlA gene product, and InlB, play important roles in the entry into cultured mammalian cells. These proteins also have extensive sequence similarities. Previously, Southern hybridization predicted the existence of an internalin multigene family. Recently, InlC, a secreted protein of 30 kDa homologous to InlA and InlB, was identified. In this work, we identified and characterized four new members of the internalin multigene family, inlC2, inlD, inlE, and inlF which encode proteins of 548, 567, 499, and 821 amino acids respectively. inlC2, inlD, and inlE are contiguous on the chromosome of L. monocytogenes EGD, whereas inlF is located in a different chromosomal region. These four inl gene products display the principal features of internalin, namely, a signal sequence, two regions of repeats (or LRR and B repeats), and a putative cell wall anchor sequence containing the sorting motif LPXTG. The four inl genes were maximally expressed albeit at a low level during early exponential growth in bacterial medium at 37 degrees C. The role of these inl genes in L. monocytogenes invasion was assessed by constructing isogenic chromosomal deletion mutants and testing them for entry into various nonphagocytic cells. Unexpectedly, the inlC2, inlD, inlE, and inlF null mutants were not affected for entry into any of the cell lines tested, raising the possibility that these genes are needed for an aspect of pathogenicity other than invasion. The identity of such an aspect remains to be determined.
Published: 1997
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47. Antibodies to the leucine-rich repeat region of internalin block entry of Listeria monocytogenes into cells expressing E-cadherin

Author: J Mengaud, Jean-Claude Mazie, Marc Lecuit, Pascale Cossart, Farida Nato, Maryse Lebrun, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: [SDV]Life Sciences [q-bio], MESH: Listeria monocytogenes, medicine.disease_cause, MESH: Cadherins, Epitope, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Antibodies, Bacterial, MESH: Animals, Listeriosis, MESH: Bacterial Proteins, 0303 health sciences, biology, Cadherins, Antibodies, Bacterial, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, Antibody, Research Article, DNA, Bacterial, medicine.drug_class, Immunology, Leucine-rich repeat, Monoclonal antibody, Microbiology, Cell Line, 03 medical and health sciences, Antigen, Listeria monocytogenes, Bacterial Proteins, Leucine, medicine, Animals, Humans, Internalin, 030304 developmental biology, Repetitive Sequences, Nucleic Acid, MESH: Epitope Mapping, MESH: Repetitive Sequences, Nucleic Acid, MESH: Humans, 030306 microbiology, MESH: DNA, Bacterial, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Cell Line, MESH: Leucine, Epitope mapping, MESH: Listeriosis, biology.protein, Parasitology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Epitope Mapping
Abstract: International audience; Internalin, a surface protein essential for entry of Listeria monocytogenes EGD into epithelial cells, was used as an antigen to raise nine monoclonal antibodies. These monoclonal antibodies recognized seven distinct epitopes which were located in three different regions of the protein. Three of them inhibited internalinmediated entry and recognized the amino-terminal leucine-rich repeat region of the protein, suggesting that this region is essential for entry.
Published: 1996
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48. Molecular and Genetic Determinants Involved in Invasion of Mammalian Cells by Listeria monocytogenes

Author: Shaynoor Dramsi, Pascale Cossart, Maryse Lebrun, Interactions Bactéries-Cellules, Institut Pasteur [Paris] (IP), Miller, V.L., and Institut Pasteur [Paris]
Subjects: Phagocyte, Yersinia Pestis, Phagocytosis, media_common.quotation_subject, [SDV]Life Sciences [q-bio], MESH: Amino Acid Sequence, Biology, medicine.disease_cause, MESH: Listeria monocytogenes, MESH: Mammals, Microbiology, 03 medical and health sciences, Listeria monocytogenes, medicine, MESH: Animals, Internalization, MESH: Phagocytosis, Pathogen, MESH: Mice, 030304 developmental biology, media_common, 0303 health sciences, MESH: Humans, MESH: Molecular Sequence Data, 030306 microbiology, Intracellular parasite, MESH: Macrophages, Listeria Monocytogenes, medicine.anatomical_structure, MESH: Listeriosis, Shigella Flexneri, biology.protein, Antibody, Signal transduction, Complement Receptor Type, Nonphagocytic Cell
Abstract: International audience; Intracellular pathogens can be artificially divided into two groups, those which are present only in phagocytes and those which also enter and replicate in nonprofessional phagocytic cells. The process of entry into nonphagocytic cells is often referred to as “parasite-directed endocytosis”or “induced phagocytosis”, since it involves the participation of both the pathogen and the host eukaryotic cell. In those cases where it has been carefully studied, interaction between a bacterial ligand and the host cell receptor results in stimulation of host signaling pathways and the subsequent cytoskeletal rearrangements necessary for uptake (BLISKA et al. 1993). Phagocytosis by professional phagocytes is generally different from “induced phagocytosis”in at least two aspects: (a) Internalization is mainly a “hostdirected” event resulting from deposition on the bacterium of antibodies or other compounds recognized by phagocyte receptors, (b) Professional phagocytes can produce potent microbicidal radicals or compounds that intracellular bacteria must circumvent, alter, or destroy in order to survive.
Published: 1996
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49. Molecular and Genetic Determinants of the Listeria monocytogenes Infectious Process

Author: Christine Kocks, Edith Gouin, Pascale Cossart, Shaynoor Dramsi, J Mengaud, B. Sheehan, André Klarsfeld, Interactions bactéries-cellules, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Dangl, J.L., and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Subjects: medicine.drug_class, [SDV]Life Sciences [q-bio], Antibiotics, MESH: Movement, Biology, MESH: Base Sequence, MESH: Heat-Shock Proteins, MESH: Virulence, medicine.disease_cause, Listeria infection, MESH: Listeria monocytogenes, Microbiology, 03 medical and health sciences, Listeria monocytogenes, Ampicillin, medicine, 030304 developmental biology, 0303 health sciences, MESH: Molecular Sequence Data, 030306 microbiology, Intercellular transport, Outbreak, medicine.disease, Listeria Monocytogenes, 3. Good health, Spotted Fever Group, Penicillin, MESH: Bacterial Toxins, MESH: Hemolysin Proteins, MESH: Listeriosis, Shigella Flexneri, Actin Assembly, MESH: Chromosome Mapping, Meningitis, Hemolytic Activity, medicine.drug
Abstract: International audience; Listeria monocytogenes was first characterized in 1926 following an outbreak of listeriosis in laboratory animals (MURRAY et al. 1926). However, it was not until the 1980s that an unambiguous link was established between the human disease and the consumption of Listeria-contaminated foodstuffs (SCHLECH et al. 1983). Immunosuppressed individuals, pregnant women, foetuses and neonates are most susceptible to Listeria infection. Human listeriosis is characterized by a high mortality rate, with clinical features including meningitis or meningo-encephalitis, septicemia, abortion, and perinatal infections (GRAY and KILLINGER 1966). If diagnosed early, listeriosis can be successfully treated by the administration of high doses of antibiotics, most frequently ampicillin or penicillin, either alone or in combination with aminoglycosides.
Published: 1994
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50. Understanding how Listeria monocytogenes targets and crosses host barriers

Author: Marc Lecuit, Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Placenta, [SDV]Life Sciences [q-bio], medicine.disease_cause, MESH: Listeria monocytogenes, MESH: Cadherins, MESH: Pregnancy, Pregnancy, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, pathogenicity, Listeriosis, MESH: Animals, Intestinal Mucosa, Pregnancy Complications, Infectious, Receptor, Pathogen, MESH: Bacterial Proteins, 0303 health sciences, General Medicine, MESH: Glutamic Acid, Cadherins, MESH: Placenta, 3. Good health, medicine.anatomical_structure, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Intestinal Mucosa, Female, MESH: Membrane Proteins, Protein Binding, Genetically modified mouse, Microbiology (medical), review, Glutamic Acid, Biology, Blood–brain barrier, Microbiology, 03 medical and health sciences, Syncytiotrophoblast, Listeria monocytogenes, Bacterial Proteins, Species Specificity, medicine, Animals, Humans, MESH: Protein Binding, MESH: Species Specificity, Internalin, MESH: Pregnancy Complications, Infectious, Animal model, 030304 developmental biology, MESH: Humans, 030306 microbiology, Trophoblast, Membrane Proteins, host barriers, Virology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, cadherin, MESH: Listeriosis, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Human listeriosis is caused by the Gram-positive bacterium Listeria monocytogenes. In humans, this pathogen has the ability to cross the intestinal, placental and blood-brain barriers, leading to gastroenteritis, maternofetal infections and meningoencephalitis, respectively. The entry of L. monocytogenes into cultured human epithelial cells is mediated by the interaction of an L. monocytogenes surface protein, internalin, with its human receptor, E-cadherin. The internalin-E-cadherin interaction is species-specific, and relies on the nature of a single amino-acid in the E-cadherin molecule, which is proline in permissive species such as humans, and glutamic acid in non-permissive species such as the mouse. In a transgenic mouse model that expresses human E-cadherin in enterocytes, internalin allows L. monocytogenes to cross the intestinal barrier. Epidemiological evidence also supports a role for internalin in human listeriosis, not only for crossing the intestinal barrier, but also for targeting and crossing the placental and blood-brain barriers. Consistent with these epidemiological data, infection with L. monocytogenes of trophoblastic cell lines, primary trophoblast cultures and human placental villous explants demonstrates that bacterial invasion of the syncytiotrophoblast barrier is mediated by the internalin-E-cadherin interaction, leading to histopathological lesions that mimic those seen in the placentas of women with listeriosis. Thus, the internalin-E-cadherin interaction that plays a key role in the crossing of the intestinal barrier in humans is also exploited by L. monocytogenes to target and cross the placental barrier. Further investigations are currently focusing on the molecular mechanisms by which L. monocytogenes targets and crosses the blood-brain barrier.
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