1. Pathfinding of Corticothalamic Axons Relies on a Rendezvous with Thalamic Projections
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Sophie Chauvet, Marie Deck, Michio Yoshida, Caroline Mailhes, Fanny Mann, Elizabeth A. Grove, Mathieu Niquille, Yutaka Yoshida, Cécile Lebrand, Maryama Keita, Ludmilla Lokmane, Sonia Garel, Institut de biologie de l'ENS Paris (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Department of Cellular Biology and Morphology, Université de Lausanne = University of Lausanne (UNIL), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Lausanne (UNIL), Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Département de Biologie - ENS Paris
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Nervous system ,Thyroid Nuclear Factor 1 ,MESH: Membrane Glycoproteins ,Semaphorins ,Mice ,0302 clinical medicine ,Molecular level ,Thalamus ,Neural Pathways ,MESH: Gene Expression Regulation, Developmental ,MESH: Animals ,MESH: Nerve Tissue Proteins ,Cerebral Cortex ,MESH: Thalamus ,0303 health sciences ,Neocortex ,Membrane Glycoproteins ,General Neuroscience ,Age Factors ,Intracellular Signaling Peptides and Proteins ,Gene Expression Regulation, Developmental ,Nuclear Proteins ,MESH: Transcription Factors ,Animals ,Axons/physiology ,Body Patterning/genetics ,Calcium-Binding Protein, Vitamin D-Dependent/metabolism ,Cerebral Cortex/cytology ,Cerebral Cortex/physiology ,Contactin 2/metabolism ,DNA-Binding Proteins/metabolism ,Embryo, Mammalian ,Gene Expression Regulation, Developmental/genetics ,Glycoproteins/genetics ,Homeodomain Proteins/genetics ,Leukocyte L1 Antigen Complex/metabolism ,Luminescent Proteins/genetics ,Luminescent Proteins/metabolism ,Membrane Glycoproteins/genetics ,Membrane Proteins/genetics ,Mice, Inbred C57BL ,Mice, Transgenic ,Nerve Tissue Proteins/genetics ,Nerve Tissue Proteins/metabolism ,Neural Pathways/physiology ,Nuclear Proteins/metabolism ,POU Domain Factors/genetics ,Repressor Proteins/metabolism ,Thalamus/cytology ,Thalamus/physiology ,Transcription Factors/genetics ,Transcription Factors/metabolism ,Tumor Suppressor Proteins/metabolism ,Wnt3A Protein/genetics ,tau Proteins/genetics ,MESH: tau Proteins ,DNA-Binding Proteins ,medicine.anatomical_structure ,Cerebral cortex ,MESH: Repressor Proteins ,Calbindin 2 ,MESH: Contactin 2 ,MESH: Luminescent Proteins ,MESH: Membrane Proteins ,Pathfinding ,Psychology ,MESH: Body Patterning ,MESH: Axons ,Cortical neuron ,MESH: Mice, Transgenic ,Neuroscience(all) ,MESH: Glycoproteins ,Nerve Tissue Proteins ,tau Proteins ,MESH: POU Domain Factors ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Waiting period ,Article ,03 medical and health sciences ,S100 Calcium Binding Protein G ,MESH: Leukocyte L1 Antigen Complex ,MESH: Mice, Inbred C57BL ,Wnt3A Protein ,MESH: Homeodomain Proteins ,medicine ,Contactin 2 ,MESH: Tumor Suppressor Proteins ,MESH: Wnt3A Protein ,MESH: Mice ,030304 developmental biology ,Body Patterning ,Glycoproteins ,Homeodomain Proteins ,MESH: Age Factors ,Tumor Suppressor Proteins ,MESH: Neural Pathways ,MESH: Calcium-Binding Protein, Vitamin D-Dependent ,MESH: Embryo, Mammalian ,Membrane Proteins ,Axons ,MESH: Cerebral Cortex ,Repressor Proteins ,Cytoskeletal Proteins ,Luminescent Proteins ,nervous system ,POU Domain Factors ,T-Box Domain Proteins ,Neuroscience ,Leukocyte L1 Antigen Complex ,MESH: Nuclear Proteins ,030217 neurology & neurosurgery ,MESH: DNA-Binding Proteins ,Transcription Factors - Abstract
International audience; Major outputs of the neocortex are conveyed by corticothalamic axons (CTAs), which form reciprocal connections with thalamocortical axons, and corticosubcerebral axons (CSAs) headed to more caudal parts of the nervous system. Previous findings establish that transcriptional programs define cortical neuron identity and suggest that CTAs and thalamic axons may guide each other, but the mechanisms governing CTA versus CSA pathfinding remain elusive. Here, we show that thalamocortical axons are required to guide pioneer CTAs away from a default CSA-like trajectory. This process relies on a hold in the progression of cortical axons, or waiting period, during which thalamic projections navigate toward cortical axons. At the molecular level, Sema3E/PlexinD1 signaling in pioneer cortical neurons mediates a "waiting signal" required to orchestrate the mandatory meeting with reciprocal thalamic axons. Our study reveals that temporal control of axonal progression contributes to spatial pathfinding of cortical projections and opens perspectives on brain wiring.
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