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5 results on '"MESH: Implants, Experimental"'

1. Beta-adrenoceptor blockade delays granulation tissue formation in polyurethane sponge implants

Author

Alexis Desmoulière, Bruna Romana-Souza, Andréa Monte-Alto-Costa, Jeanine Salles dos Santos, Department of histology and Embryology, State University of Rio de Janeiro, Université de Limoges (UNILIM), Homéostasie Cellulaire et Pathologies (HCP), and Université de Limoges (UNILIM)-CHU Limoges-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)

Subjects
Male, Pathology, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Polyurethanes, H&E stain, MESH: Polyurethanes, 030207 dermatology & venereal diseases, chemistry.chemical_compound, 0302 clinical medicine, Implants, Experimental, MESH: Collagen, MESH: Animals, 0303 health sciences, Granulation tissue, MESH: Adrenergic beta-Antagonists, Immunohistochemistry, MESH: Granulation Tissue, 3. Good health, medicine.anatomical_structure, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Collagen, Myofibroblast, medicine.drug, Blood vessel, medicine.medical_specialty, Histology, MESH: Rats, Adrenergic beta-Antagonists, MESH: Receptors, Adrenergic, beta, MESH: Implants, Experimental, Dermatology, Propranolol, Pathology and Forensic Medicine, 03 medical and health sciences, Phentolamine, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Receptors, Adrenergic, beta, medicine, Animals, Rats, Wistar, Sirius Red, Adrenergic alpha-Antagonists, 030304 developmental biology, business.industry, MESH: Receptors, Adrenergic, alpha, MESH: Immunohistochemistry, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, MESH: Rats, Wistar, Receptors, Adrenergic, alpha, Atenolol, MESH: Male, Rats, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, chemistry, Granulation Tissue, MESH: Adrenergic alpha-Antagonists, business, [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology
Abstract

International audience; BACKGROUND: The role of adrenoceptors in granulation tissue formation is not well understood. The aim of this study was to investigate the effects of alpha- and beta-adrenoceptor blockade on granulation tissue development using polyurethane (PU) implants in the rat. METHODS: Animals were treated orally with propranolol (beta1- and beta2-antagonist), atenolol (beta1-antagonist) or phentolamine (alpha1- and alpha2-antagonist) until euthanasia. The control group received only water. All animals received subcutaneous implants of PU sponges. After 14 days, implants were collected, formalin-fixed and paraffin-embedded. Sections were stained with hematoxylin and eosin and Sirius red and immunostained for CD68 and alpha-smooth muscle actin. RESULTS: The number of inflammatory cells and the volume density of myofibroblasts and blood vessels were lower in the control group than in the propranolol- and atenolol-treated groups. The collagen fiber score was greater in the control group than in the propranolol- and atenolol-treated groups. The inflammatory infiltrate, collagen fiber score, blood vessel density or myofibroblast differentiation was not affected by phentolamine. The percentage of fibrovascular invasion was greater in the antagonist-treated groups than in the control group. CONCLUSIONS: Blockade of beta1- and beta2-adrenoceptors, but not alpha-adrenoceptors, impairs granulation tissue development in PU implants due to interference with the inflammatory response.

Published
2009

2. A glucose biofuel cell implanted in rats

Author

Fabien Giroud, Aymeric Pellissier, Philippe Cinquin, Simon Mazabrard, Serge Cosnier, Jean-Pierre Alcaraz, Chantal Gondran, Paolo Porcu, Karine Gorgy, François Lenouvel, Stephane Mathe, François Boucher, Gestes Medico-chirurgicaux Assistés par Ordinateur (TIMC-IMAG-GMCAO), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF), Physiologie cardio-Respiratoire Expérimentale Théorique et Appliquée (TIMC-IMAG-PRETA), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Plastes et Différenciation Cellulaire (PDC), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), ANR (Agence Nationale de la Recherche), EMERGENCE (Rhone-Alpes Region program) and Floralis (UJF's subsidiary), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, MESH: Oxidation-Reduction, MESH: Hydrogen-Ion Concentration, Time Factors, Biochemistry/Membrane Proteins and Energy Transduction, Bioelectric Energy Sources, Ubiquinone, lcsh:Medicine, 02 engineering and technology, 7. Clean energy, 01 natural sciences, Chloride, Biotechnology/Biocatalysis, Implants, Experimental, Urea, Glucose oxidase, MESH: Animals, MESH: Ubiquinone, Enzymatic biofuel cell, lcsh:Science, chemistry.chemical_classification, Chemistry/Physical, Inorganic, and Analytical Chemistry, Multidisciplinary, biology, Hydrogen-Ion Concentration, 021001 nanoscience & nanotechnology, Urease, MESH: Glucose, Biochemistry, Biophysics/Membrane Proteins and Energy Transduction, Physiology/Integrative Physiology, Biotechnology/Bioengineering, 0210 nano-technology, MESH: Bioelectric Energy Sources, Oxidation-Reduction, Catechol Oxidase, Research Article, medicine.drug, MESH: Rats, MESH: Urease, Biochemistry/Biocatalysis, MESH: Implants, Experimental, Chemistry/Applied Chemistry, MESH: Catechol Oxidase, 010402 general chemistry, MESH: Prosthesis Implantation, Redox, Prosthesis Implantation, Glucose Oxidase, medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Animals, Chemistry/Biochemistry, Biophysics/Biocatalysis, Rats, Wistar, Catechol oxidase, Electrodes, MESH: Urea, lcsh:R, MESH: Time Factors, MESH: Electrodes, MESH: Rats, Wistar, MESH: Male, Rats, 0104 chemical sciences, Anode, Glucose, Enzyme, chemistry, biology.protein, Biophysics, MESH: Glucose Oxidase, Surgery, lcsh:Q
Abstract

International audience; Powering future generations of implanted medical devices will require cumbersome transcutaneous energy transfer or harvesting energy from the human body. No functional solution that harvests power from the body is currently available, despite attempts to use the Seebeck thermoelectric effect, vibrations or body movements. Glucose fuel cells appear more promising, since they produce electrical energy from glucose and dioxygen, two substrates present in physiological fluids. The most powerful ones, Glucose BioFuel Cells (GBFCs), are based on enzymes electrically wired by redox mediators. However, GBFCs cannot be implanted in animals, mainly because the enzymes they rely on either require low pH or are inhibited by chloride or urate anions, present in the Extra Cellular Fluid (ECF). Here we present the first functional implantable GBFC, working in the retroperitoneal space of freely moving rats. The breakthrough relies on the design of a new family of GBFCs, characterized by an innovative and simple mechanical confinement of various enzymes and redox mediators: enzymes are no longer covalently bound to the surface of the electron collectors, which enables use of a wide variety of enzymes and redox mediators, augments the quantity of active enzymes, and simplifies GBFC construction. Our most efficient GBFC was based on composite graphite discs containing glucose oxidase and ubiquinone at the anode, polyphenol oxidase (PPO) and quinone at the cathode. PPO reduces dioxygen into water, at pH 7 and in the presence of chloride ions and urates at physiological concentrations. This GBFC, with electrodes of 0.133 mL, produced a peak specific power of 24.4 microW mL(-1), which is better than pacemakers' requirements and paves the way for the development of a new generation of implantable artificial organs, covering a wide range of medical applications.

Published
2010

3. Factorial design optimization and in vivo feasibility of poly(epsilon-caprolactone)-micro- and nanofiber-based small diameter vascular grafts

Author

Jean-Christophe Tille, Benjamin Nottelet, D. Mandracchia, Robert Gurny, Erman Pektok, Beat H. Walpoth, Michael Möller, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Cardiovascular Research, Service of Cardiovascular Surgery, University Hospital of Geneva, Dipartimento Farmaco Chimico, Università degli studi di Bari Aldo Moro (UNIBA), Department of Clinical Pathology, Department of Pharmaceutics and Biopharmaceutics, and School of Pharmaceutical Sciences, University of Geneva, University of Lausanne

Subjects
Polyesters/pharmacology, Pilot Projects, MESH: Solvents, 02 engineering and technology, ddc:616.07, MESH: Tissue Engineering, biodegradable polymers, vascular grafts, electrospinning, chemistry.chemical_compound, MESH: Blood Vessel Prosthesis, Tissue engineering, Blood Vessels/cytology/transplantation/ultrastructure, Implants, Experimental, Tissue Engineering/methods, MESH: Animals, MESH: Angiography, ddc:615, Tensile Strength/drug effects, ddc:617, Metals and Alloys, Angiography, Factorial experiment, 021001 nanoscience & nanotechnology, Electrospinning, MESH: Polyesters, Polycaprolactone, 0210 nano-technology, Nanostructures/chemistry, Materials science, MESH: Rats, Surface Properties, Polyesters, 0206 medical engineering, Biomedical Engineering, MESH: Implants, Experimental, Surface Properties/drug effects, Biomaterials, MESH: Nanostructures, Blood vessel prosthesis, Tensile Strength, Ultimate tensile strength, Animals, MESH: Tensile Strength, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, MESH: Surface Properties, Tissue Engineering, MESH: Blood Vessels, MESH: Pilot Projects, 020601 biomedical engineering, Biodegradable polymer, Nanostructures, Blood Vessel Prosthesis, Rats, chemistry, Nanofiber, Ceramics and Composites, Solvents, Blood Vessels, Feasibility Studies, MESH: Feasibility Studies, Biomedical engineering
Abstract

International audience; Because of the severe increase of mortality by cardiovascular diseases, there has been rising interest among the tissue-engineering community for small-sized blood vessel substitutes. Here we present small diameter vascular grafts made of slow degradable poly(epsilon-caprolactone) nanofibers obtained by electrospinning. The process was optimized by a factorial design approach that led to reproducible grafts with inner diameters of 2 and 4 mm, respectively. Fiber sizes, graft morphology, and the resulting tensile stress and tensile strain values were studied as a function of various parameters in order to obtain optimal vascular grafts for implantation after gamma-sterilization. The influence of polymer concentration, solvent, needle-collector distance, applied voltage, flow rate, and spinning time has been studied. Consequently, an optimized vascular graft was implanted as an abdominal aortic substitute in nine rats for a feasibility study. Results are given following up a 12-week implantation period showing good patency, endothelization, and cell ingrowth.

Published
2009

4. Inflammatory reaction in rats muscle after implantation of biphasic calcium phosphate micro particles

Author

Pierre Layrolle, Nicolas Josselin, Pierre Weiss, Borhane H. Fellah, Daniel Chappard, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Groupe d'Études Remodelage Osseux et bioMatériaux (GEROM), Université d'Angers (UA), and Layrolle, Pierre

Subjects
Calcium Phosphates, MESH: Inflammation, MESH: Materials Testing, Scanning electron microscope, Cell Culture Techniques, Sintering, Biocompatible Materials, 02 engineering and technology, Bone tissue, MESH: Tissue Engineering, chemistry.chemical_compound, MESH: Structure-Activity Relationship, Implants, Experimental, MESH: Biocompatible Materials, Fibrosis, Materials Testing, MESH: Animals, 0303 health sciences, 021001 nanoscience & nanotechnology, Biphasic calcium phosphate, MESH: Calcium Phosphates, medicine.anatomical_structure, 0210 nano-technology, Muscle tissue, Materials science, MESH: Rats, Surface Properties, Biomedical Engineering, Biophysics, Mineralogy, Bioengineering, MESH: Implants, Experimental, Biomaterials, 03 medical and health sciences, Structure-Activity Relationship, Polymethacrylic Acids, medicine, Animals, Rats, Wistar, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, 030304 developmental biology, Inflammation, MESH: Surface Properties, MESH: Cell Culture Techniques, MESH: Polymethacrylic Acids, Tissue Engineering, Histology, MESH: Rats, Wistar, medicine.disease, Phosphate, Rats, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, chemistry
Abstract

Several studies have shown that macro micro porous bioceramics ectopically implanted promote bone tissue formation. This study aims at investigating the inflammatory response towards biphasic calcium phosphate (BCP) ceramic micro particles. BCP composed of hydroxyapatite (HA) and beta-tricalcium phosphate, HA/beta -TCP ratio of 50/50, were prepared by sintering at 1200 degrees C for 5 h. After crushing, 3 fractions of BCP micro particles < 20, 40-80 and 80-200 micro m were sieved. The micro particles were carefully characterized by using X-ray diffraction (XRD), scanning electron microscopy (SEM) and laser scattering. The inflammatory reactions induced by BCP micro particles implanted in quadriceps muscles of rats for 7, 14 and 21 days were studied by histology (n = 8/group). A fibrous tissue encapsulation of the BCP micro particles implanted in muscle tissue was observed and fibrosis was similar for the 3 groups of micro particles. The comparison of the cellular response indicated that the total number of cells was significantly higher for BCP < 20 micro m than for 40-80 and 80-200 micro m (p < 0.0001). The number of macrophages was relatively higher for the smallest than for the intermediate and largest fractions (p < 0.0001). The relative percentage of giant cells was higher for the intermediate and largest size of particles than for the smallest. The number of lymphocytes was comparable for the 3 fractions and after the 3 delays. Therefore, the BCP micro particles < 20 micro m initiated an inflammatory response which might play an important role in osteogenesis.

Published
2007

5. Nacre/bone interface changes in durable nacre endosseous implants in sheep

Author

Olivier Delattre, Sophie Berland, Sandrine Borzeix, Evelyne Lopez, Yves Catonné, Biologie des organismes marins et écosystèmes (BOME), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Muséum national d'Histoire naturelle (MNHN), and Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, MESH: Materials Testing, Biophysics, Implantation Site, MESH: Sheep, Bioengineering, Biocompatible Materials, MESH: Bone Substitutes, MESH: Implants, Experimental, 02 engineering and technology, Bone healing, Bone tissue, Biomaterials, 03 medical and health sciences, Implants, Experimental, MESH: Biocompatible Materials, Osseointegration, MESH: Bone Transplantation, Surface change, Materials Testing, medicine, Animals, Endosseous implants, MESH: Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Femur, 030304 developmental biology, 0303 health sciences, Bone Transplantation, Sheep, MESH: Osseointegration, Anatomy, 021001 nanoscience & nanotechnology, MESH: Mollusca, Biodegradation kinetics, MESH: Femur, medicine.anatomical_structure, Key factors, Mechanics of Materials, Mollusca, Bone Substitutes, Ceramics and Composites, Female, Implant, 0210 nano-technology, MESH: Female, Biomedical engineering
Abstract

International audience; Raw nacre implants persist even after 9 months of implantation into bone tissue in sheep. However the nacre surface undergoes a limited biodegradation process. Smooth-surfaced nacre implants were seen to become microporous after implantation. The results of these long-term, in vivo studies show that the overall process involves bone-resorbing cells, relies on a two-phase mechanism and may correspond to a regulation process. The rate of surface change depends on the bone implantation site and the nacre/bone interaction. The in vivo biodegradability of nacre is a highly variable parameter. The size and shape of the implanted nacre and the cellular environment of the implant are key factors in determining the biodegradation kinetics of the nacre in a living system.

Published
2005

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