1. A Transgenic Model for Listeriosis: Role of Internalin in Crossing the Intestinal Barrier
- Author
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Michel Huerre, Jean Lefort, Sandrine Vandormael-Pournin, Pierre Gounon, Charles Babinet, Marc Lecuit, Catherine Dupuy, Pascale Cossart, Interactions Bactéries-Cellules (UIBC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Institut Pasteur [Paris], Pharmacologie Cellulaire, Histopathologie, Microscopie électronique, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), and Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
Male ,MESH: Neoplasm Proteins ,MESH: Intestine, Small ,MESH: Spleen ,[SDV]Life Sciences [q-bio] ,Colony Count, Microbial ,MESH: Lymph Nodes ,MESH: Virulence ,medicine.disease_cause ,MESH: Listeria monocytogenes ,MESH: Cadherins ,Transgenic Model ,Mice ,MESH: Enterocytes ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,MESH: Fatty Acid-Binding Protein 7 ,Intestine, Small ,Listeriosis ,MESH: Animals ,Transgenes ,MESH: Nerve Tissue Proteins ,Intestinal Mucosa ,Receptor ,Promoter Regions, Genetic ,MESH: Bacterial Proteins ,0303 health sciences ,Multidisciplinary ,Virulence ,Cadherins ,3. Good health ,Neoplasm Proteins ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Liver ,MESH: Intestinal Mucosa ,Fatty Acid-Binding Protein 7 ,MESH: Bacterial Translocation ,Genetically modified mouse ,MESH: Mice, Transgenic ,Transgene ,Guinea Pigs ,Mice, Transgenic ,Nerve Tissue Proteins ,MESH: Transgenes ,MESH: Carrier Proteins ,Biology ,Fatty Acid-Binding Proteins ,MESH: Fatty Acid-Binding Proteins ,Microbiology ,MESH: Guinea Pigs ,Guinea pig ,03 medical and health sciences ,Listeria monocytogenes ,Bacterial Proteins ,MESH: Promoter Regions, Genetic ,medicine ,Animals ,Humans ,Internalin ,MESH: Tumor Suppressor Proteins ,MESH: Mice ,MESH: Colony Count, Microbial ,030304 developmental biology ,MESH: Humans ,030306 microbiology ,Tumor Suppressor Proteins ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,MESH: Male ,Disease Models, Animal ,Enterocytes ,MESH: Listeriosis ,Bacterial Translocation ,Immunology ,Lymph Nodes ,MESH: Disease Models, Animal ,Carrier Proteins ,Spleen ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,MESH: Liver - Abstract
Listeria monocytogenes is responsible for severe food-borne infections, but the mechanisms by which bacteria cross the intestinal barrier are unknown. Listeria monocytogenes expresses a surface protein, internalin, that interacts with a host receptor, E-cadherin, to promote entry into human epithelial cells. Murine E-cadherin, in contrast to guinea pig E-cadherin, does not interact with internalin, excluding the mouse as a model for addressing internalin function in vivo. In guinea pigs and transgenic mice expressing human E-cadherin, internalin was found to mediate invasion of enterocytes and crossing of the intestinal barrier. These results illustrate how relevant animal models for human infections can be generated.
- Published
- 2001
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